Development Of A Targeted Mass Spectrometry Method For The Detection And Quantification Of Peanut Protein In Incurred Food Matrices,
2022
University of Nebraska-Lincoln
Development Of A Targeted Mass Spectrometry Method For The Detection And Quantification Of Peanut Protein In Incurred Food Matrices, Sara Schlange
Department of Food Science and Technology: Dissertations, Theses, and Student Research
The unintentional presence of peanut in food products through allergen cross-contact is a considerable safety concern for peanut-allergic individuals. The food industry monitors for this contamination using immunoassays; however, these detection methods demonstrate issues with recovery and accurate quantification of allergenic protein when analyzing processed, complex food matrices. Of particular concern is the deficit in immunoassay-based detection and quantification of peanut in cookie and dark chocolate matrices, as the unintentional presence of peanut has been observed in these food products. A liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for the detection and quantification of peanut protein in cookie and …
Safety Assessment Of Novel Foods And Food Proteins,
2022
University of Nebraska-Lincoln
Safety Assessment Of Novel Foods And Food Proteins, Niloofar Moghadam Maragheh
Department of Food Science and Technology: Dissertations, Theses, and Student Research
The global food market needs to grow and supply food demand to feed the growing world population. Alternative food proteins, including novel sources of safe foods and ingredients, are the candidates that could provide more environmentally sustainable choices, animal welfare, and consumers health. Novel foods and food proteins must undergo premarket safety evaluations including allergenicity assessment to reduce the risk of cross-reactivity with known allergens and uncharacterized risk to food allergic individuals. This research addressed the safety assessment of some novel foods and food ingredients using the study of stability of proteins in pepsin and sequence identity analysis in the …
Identification Of Gut Microbiome Composition Responsible For Gas Production,
2022
University of Nebraska-Lincoln
Identification Of Gut Microbiome Composition Responsible For Gas Production, Erasme Mutuyemungu
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Gas production remains a significant barrier to increasing intake of dietary fiber-containing foods for many consumers. Therefore, this thesis is comprised of two studies focusing on the role of the gut microbiome in contributing to gas production during fermentation of dietary components. Previous studies have reported significant correlations between Megasphaera elsdenii and gas production during fermentation of pulses. Therefore, the objective of the first study was to determine the role of M. elsdenii in gas production by the microbiome during fermentation of foods commonly associated with gas production. Human fecal microbiomes were separated based on the presence (Me+) or absence …
Evaluation Of Wheat-Specific Peptide Targets For Use In The Development Of Elisa And Mass Spectrometry-Based Detection Methods,
2022
University of Nebraska-Lincoln
Evaluation Of Wheat-Specific Peptide Targets For Use In The Development Of Elisa And Mass Spectrometry-Based Detection Methods, Jessica Humphrey
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Individuals with IgE-mediated wheat allergy and celiac disease must avoid the consumption of wheat. Those with celiac disease must also avoid rye and barley in addition to wheat as these make up the gluten-containing grains. To ensure products are accurately labeled, ELISA methods may be used to monitor compliance with food allergen and gluten-free regulations. Currently, available ELISA methods for wheat detection utilize monoclonal antibodies that target peptides from the gluten fraction of proteins, and therefore, also detect rye and barley. Limitations such as differing antibody reactivity and unequal gluten content among wheat, rye, and barley have led to inconsistent …
Occurrence Of Hydroxyproline In Proteomes Of Higher Plants,
2022
University of Nebraska-Lincoln
Occurrence Of Hydroxyproline In Proteomes Of Higher Plants, Olivia Huffman
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Food allergies affect millions of individuals across the United States and worldwide. Peanut allergies are among the most severe food allergies because of their potentially life-threatening symptoms and lifelong persistence. Potent peanut allergen, Ara h 2, is known to contain an amino acid motif containing the posttranslational modification, hydroxyproline (HyP). HyP is associated with immunogenic response when present both in Ara h 2 and in timothy grass pollen allergen, Phl p 1. To further explore the presence of HyP in higher plants and specifically to investigate its potential presence in commonly allergenic plants, a study of 26 plant seeds was …
Lactic Acid Bacteria Isolated From Fresh Vegetable Products: Potential Probiotic And Postbiotic Characteristics Including Immunomodulatory Effects,
2022
United Arab Emirates University
Lactic Acid Bacteria Isolated From Fresh Vegetable Products: Potential Probiotic And Postbiotic Characteristics Including Immunomodulatory Effects, Fatima Khaled Alameri
Theses
The ability to perform effectively in the gastrointestinal system (GIT) is one of the most significant criteria for selecting the best probiotic bacteria. Thus, the present study aimed to investigate the potential probiotic characteristics of some selected lactic acid bacteria (LAB) isolated from vegetable products. Probiotic characteristics included tolerance to acid and bile, cholesterol removing ability, bile salt hydrolysis, resistance against lysozyme and antibiotics, production of exopolysaccharides (EPS), antimicrobial and hemolytic activities, and cell surface characteristics (auto-aggregation, co-aggregation, and hydrophobicity). Out of 46 isolates, 17 isolates that exhibited remarkable survivability under gastrointestinal conditions were selected for further analysis. Almost all …
Spectrophotometric Methods For Measurement Of Antioxidant Activity In Food And Pharmaceuticals,
2022
Edith Cowan University
Spectrophotometric Methods For Measurement Of Antioxidant Activity In Food And Pharmaceuticals, Marios C. Christodoulou, Jose C. Orellana Palacios, Golnaz Hesami, Shima Jafarzadeh, José M. Lorenzo, Rubén Domínguez, Andres Moreno, Milad Hadidi
Research outputs 2022 to 2026
In recent years, there has been a growing interest in the application of antioxidants in food and pharmaceuticals due to their association with beneficial health effects against numerous oxidative-related human diseases. The antioxidant potential can be measured by various assays with specific mechanisms of action, including hydrogen atom transfer, single electron transfer, and targeted scavenging activities. Understanding the chemistry of mechanisms, advantages, and limitations of the methods is critical for the proper selection of techniques for the valid assessment of antioxidant activity in specific samples or conditions. There are various analytical techniques available for determining the antioxidant activity of biological …
Acrylamide Mitigation By Novel Lactic Acid Bacteria And Bifidobacteria Isolated From Various Food Products: Optimization Of Mitigation, Effect Of Simulated Gastrointestinal Conditions, And Potential Mechanism,
2022
United Arab Emirates University
Acrylamide Mitigation By Novel Lactic Acid Bacteria And Bifidobacteria Isolated From Various Food Products: Optimization Of Mitigation, Effect Of Simulated Gastrointestinal Conditions, And Potential Mechanism, Amal Salem Albedwawi
Dissertations
Acrylamide is a toxic compound that is formed in heated carbohydrate-rich food. Scientists have analyzed acrylamide levels before, during, and after processing in order to study the mitigation strategies and, due to the lack of knowledge, this study aimed to: 1) screen 120 newly isolated LAB for their acrylamide removal; 2) optimize the conditions for 6 selected strains of pH, temperature, time and salt, using the Box-Behnken design (BBD), and analyze their acrylamide removal levels; 3) investigate acrylamide removal abilities of the selected LAB isolates under in-vitro digestion conditions, using the INFO-GEST 2.0 model; and 4) examine the mechanism of …
Influence Of Hesperidin On The Physico-Chemical, Microbiological, And Sensory Characteristics Of Frozen Yogurt,
2022
Louisiana State University and Agricultural and Mechanical College
Influence Of Hesperidin On The Physico-Chemical, Microbiological, And Sensory Characteristics Of Frozen Yogurt, Roberto Antonio Cedillos Hernández
LSU Master's Theses
Frozen yogurts contain yogurt culture bacteria which might impart health benefits to its consumers. Global frozen yogurt market sales are expected to grow 4.8% by 2028 which represents an important opportunity for the industry, consumers, and researchers. Polyphenols are metabolites found in plants which have antioxidant and anti-inflammatory properties and might prevent chronical diseases such as cancer, diabetes, and cardiovascular diseases. The objective of this study was to elucidate the effect of the polyphenol hesperidin on the physico-chemical, microbiological, and sensory characteristics of frozen yogurts. Hesperidin was incorporated in frozen yogurt at three concentrations (500, 250 and 125 mg/90g of …
An Active Biodegradable Layer-By-Layer Film Based On Chitosan-Alginate-Tio2 For The Enhanced Shelf Life Of Tomatoes,
2022
Technological University Dublin
An Active Biodegradable Layer-By-Layer Film Based On Chitosan-Alginate-Tio2 For The Enhanced Shelf Life Of Tomatoes, Kalpani Y. Perera, Shubham Sharma, Brendan Duffy, Shiviani Pathania, Amit K. Jaiswal, Swarma Jaiswal
Articles
This work aims at developing biodegradable active chitosan-alginate layer-by-layer bio-nanocomposite film with TiO2NPs using the solvent casting method followed by CaCl2 crosslinking for food packaging applications. The developed films enhanced the tensile strength and elongation at break by 14.76 and 2 folds (p < 0.05) respectively. The UV barrier properties of CH-SA-0.3%TiO2 film increased by 88.6%, while the film transparency decreased by 87.23%. All films showed antimicrobial activity against foodborne pathogens E. coli, S. aureus, S. typhi, and L. monocytogene. The film with 0.1%TiO2 showed the complete killing of gram-positive bacteria. The CH-SA-0.1%TiO2 film was completely biodegraded during the …
Patterns Of Population Structure And Introgression Among
Recently Differentiated Drosophila Melanogaster
Populations,
2022
University of North Carolina, Yale University
Patterns Of Population Structure And Introgression Among Recently Differentiated Drosophila Melanogaster Populations, Jenn M. Coughlan, Andrius J. Dagilis, Antonio Serrato-Capuchina, Hope Elias, David Peede, Kristin Isbell, Dean M. Castillo, Brandon S. Cooper, Daniel R. Matute
Nebraska Cooperative Extension: Faculty and Staff Publications
Despite a century of genetic analysis, the evolutionary processes that have generated the patterns of exceptional genetic and phenotypic variation in the model organism Drosophila melanogaster remains poorly understood. In particular, how genetic variation is partitioned within its putative ancestral range in Southern Africa remains unresolved. Here, we study patterns of population genetic structure, admixture, and the spatial structuring of candidate incompatibility alleles across a global sample, including 223 new accessions, predominantly from remote regions in Southern Africa. We identify nine major ancestries, six that primarily occur in Africa and one that has not been previously described. We find evidence …
Workspace Simulation And Trajectory Planning Of Food Handling Robot,
2022
Industrial Manipulator Control and Reliability Technology Innovation Center of Hebei,Cangzhou, Hebei 061001 , China ；Industrial Manipulator Control and Reliability TechnologyInnovation Center of Cangzhou, Cangzhou, Hebei 061001 , China ；School of Electrical Engineering,Hebei University of Water Resources and Electric Engineering, Cangzhou, Hebei 061001 , China
Workspace Simulation And Trajectory Planning Of Food Handling Robot, Cai Guo-Qing, Hao Rui-Lin, Zhou Li-Jie, Shi Yan
Food and Machinery
Objective: Analyzed the workspace of 6-DOF food handling robot and planed the motion trajectory, so as to provide basis for the layout of food handling platform in workstation and the motion control of robot. Methods: Composition and structure of the robot were analyzed, the kinematics model was established, and the kinematics model was verified by MATLAB. Based on this, the workspace of the robot was modeled and solved, the working area of the robot was obtained, and the reasonable layout position of the food handling table was given; combined with the requirements of food handling task, a three-stage …
Effect Of The Electric Field Treatment On The Pullulanase-Catalyzed Hydrolysis Of Waxy Rice Starch,
2022
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095 , China
Effect Of The Electric Field Treatment On The Pullulanase-Catalyzed Hydrolysis Of Waxy Rice Starch, Chen Cai-Wen, Tian Jia-Ning, Yu Kun-Zheng, Li Dan-Dan
Food and Machinery
Objective:This study aimed to classify the mechanism of pullulanase-catalyzed hydrolysis of waxy rice starch under electric field treatment, by monitoring the changes in hydrolysis efficiency, pullulanase activity, and the structure and properties of waxy rice starch. Methods:The hydrolysis efficiency was determined, and the structure and thermal properties of hydrolysates were characterized, by using scanning electron microscope, X-ray diffractometer, differential scanning calorimeterand rapid viscosity analyzer. Results:Under the control of the following conditions, electric field with intensities ≤2.5 V/cm could increase pullulanase activity, accelerate the hydrolysis, induce the pores and cracks on the granule surface, increase the relative crystallinity, …
Effects Of Microwave Aging On The Content Of Phenols,Organic Acids And Esters In Black Rice Wine,
2022
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023 , China
Effects Of Microwave Aging On The Content Of Phenols,Organic Acids And Esters In Black Rice Wine, Tang Lin-Yue, Yuan Jiang-Feng, Lai Yu-Ting, Gong Ming-Gui, Sun Jian-Rui
Food and Machinery
Objective:This study aimed to solve the problems of time consuming and high cost in the natural aging process. Methods: Black rice wine was treated with different microwave power, time and temperature. The contents of main phenols, organic acids, and esters in black rice wine treated by microwave technology were analyzed. Results: Microwave treatment could increase the contents of total esters, protocatechuic acid, gallic acid, syringic acid, acetic acid, ethyl valerate and ethyl acetate, reduce the contents of total phenols, total acids, caffeic acid, citric acid, tartaric acid, lactic acid and ethyl lactate, and have little effect on the contents …
Prediction Of Apple Sugar Content Based On Correlation Of Characteristic Gray Series In Darkroom System,
2022
College of Electronic Engineering & Artificial Intelligence, South China Agricultural University, Guangzhou, Guangdong 510642 , China
Prediction Of Apple Sugar Content Based On Correlation Of Characteristic Gray Series In Darkroom System, Ma Cheng-Tong, Li Jun-Wen, Ouyang Hao-Yi, Tan Sui-Yan, Yang Chu-Ping
Food and Machinery
Objective:In order to realize the detection of apple sugar content, a nondestructive prediction method of apple sugar content based on the characteristic gray series of apple reflection spot image in darkroom system is proposed. Methods:The laser with the peak wavelength of 670 nm absorbed by the apple was used as the illumination light source, which was incident from the illumination port of the integrating sphere. The apple sample was placed at the sample port of the integrating sphere, and the reflection spot of the apple sample was obtained at the measuring port of the integrating sphere. Through the …
Effect Of Microbial Nitrosation Inhibitors On The Formation Of Flavor Substances In Air-Dried Sausage,
2022
College of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384 , China
Effect Of Microbial Nitrosation Inhibitors On The Formation Of Flavor Substances In Air-Dried Sausage, Chen Yuan-Yuan, Ma Kai-Hua, Zhang Yu-Ting, Zheng Xiang-Fei, Zhang Xin-Yu, Ma Li-Zhen
Food and Machinery
Objective: To study the effects of microbial nitrification inhibitors on the formation and changes of flavors in air-dried sausages. Methods: Four groups of air-dried sausages were designed: MNI group: 0.05% of microbial nitrosation inhibitors (MNI) were added; MNIP group: 0.05% MNI and an inoculation of PRO-MIX5 commercial starter (Staphylococcus xylose, Lactobacillus sake,and L. plantarum) were added; FBFAP group: fermented beef flavorings and compound antioxidants were added, and the sausages were inoculated with the PRO-MIX5 commercial starter; and CK group: blank control (CK). The flavor of air-dried sausages was determined by an amino acid analyzer, Heracles iii ultra-fast gas phase electronic …
Analysis Of Volatile Components In Different Parts Of Lavender Based On Hs-Spme-Gc-Ms,
2022
College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, Xinjiang 830054 , China ；Xinjiang Institute of Analysis and Testing, Urumqi, Xinjiang 830011 , China
Analysis Of Volatile Components In Different Parts Of Lavender Based On Hs-Spme-Gc-Ms, Chen Guo-Tong, Lu Yun, Ma Ping-Ping, Fan Rui, Yang Zhong, Cao Xu
Food and Machinery
Objective: This study aimed to obtain the volatile components of different parts of lavender in natural condition and provide theoretical basis for quality evaluation and rational utilization of lavender varieties.Methods: Orthogonal experiment was used to optimize the headspace solid phase microextraction (HS-SPME-GC-MS) conditions, and the volatile components of the root, stem, leaf and flower of the representative lavender species France blue were analyzed under the optimal conditions.Results: The optimal conditions of HS-SPME were extraction time 60 min, equilibrium time 20 min and desorption time 5 min. Under the control of these conditions, a total of 36 chemical components …
Effect Of Tobacco Moisture Content On Releases Of Main Components In Heated Cigarette Aerosol,
2022
R&D Center of China Tobacco Yunnan Industrial Co., Ltd., Kunming, Yunnan 650231 , China ；Xiangtan University, Department of Environment and Resources, Xiangtan, Hunan 411105 , China
Effect Of Tobacco Moisture Content On Releases Of Main Components In Heated Cigarette Aerosol, Zhang Hu, Duan Yuan-Xing, Zhao Yang, Li Shi-Wei, Yang Liu
Food and Machinery
Objective: To investigate the effect of moisture content of heated cigarette on releases of aerosol main components. Methods: Three different types of heated cigarettes were used to study the effects of moisture content of cigarette sticks on the release of main aerosol components by gas chromatography. Results: ① The equilibrium humidity was a positive correlation between the moisture contents of tobacco materials and releases of total aerosol particulate matters. ② The moisture contents of heated cigarettes stored at different equilibrium humilities had a great effect on releases of glycerol and nicotine in aerosol, but a small effect on release of …
Effects Of Nacl Stress And Cacl2 Supplemental On Phenolic Accumulation And Antioxidant Activity In Common Vetch Sprouts,
2022
Beijing Key Laboratory of Functional Food from Plant Resources, College of Science and Nutritional Engineering, China Agricultural University, Beijing 100083 , China
Effects Of Nacl Stress And Cacl2 Supplemental On Phenolic Accumulation And Antioxidant Activity In Common Vetch Sprouts, Zhao Qi-Yan, Tang Ning, Zhao De-Chen, Zhang Bo-Ya, Cheng Yong-Qiang
Food and Machinery
Objective: The study aimed to develop the vegetable value of common vetch (CV) and explore the effects of salt solution on phenolic accumulation and antioxidant activity in CV sprouts.Methods: The effects of NaCl stress and supplemental CaCl2 on free and combined phenolic accumulation in CV sprouts were analysed by UPLC in comparison with pea sprouts, soybean sprouts and mung bean sprouts. The changes of key enzyme activities and gene relative expression levels of PAL, C4H and 4CL, antioxidant enzyme activities and antioxidant activities were investigated under NaCl and CaCl2 treatments.Results: The total phenolics increased from (10.63±0.40) mg GAE/g …
Rapid Determination Of Matrine And Oxymatrine In Honey,
2022
Hunan Provincial Institute of Product and Goods Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117 , China
Rapid Determination Of Matrine And Oxymatrine In Honey, Jing Hui-Hua, Xiang Jun, Jiang Deng-Hui, Feng Yan-Ying, Wang Yun-Hao, Wang Liang-Liang
Food and Machinery
Objective:This study aimed to establish a new method for the determination of matrine and oxymatrine in honey by UPLC-MS/MS. Methods: The honey was dissolved in water, and the liquid-liquid extraction pretreatment technology was adopted. The extraction was achieved using ammonia-acetonitrile, with the addition of sodium sulfate to induce a salting out effect. Chromatographic separation was achieved on Inspire HILIC column in the mobile phase composition of acetonitrile and 0.1% formic acid5 mmol/L ammonium acetate buffer. Mass Spectrometry was carried out under the multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI+) and external standard method for quantitative analysis. …
