Directional Immobilization Of Mutant Β-Agarase Aga 50d Onto Magnetic Nanoparticles And Its Thermal Stability,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Directional Immobilization Of Mutant Β-Agarase Aga 50d Onto Magnetic Nanoparticles And Its Thermal Stability, Qiaoling Xie, Jie Long, Long Chen, Chao Qiu, Xing Zhou, Zhengyu Jin
Journal of Food Science and Biotechnology
[Objective ] This study aims to achieve site-directed immobilization of β-agarase at targeted residues,thereby enhancing its thermal stability and operational performance.[Method ] Computer-aided design was employed to selectively immobilize the mutant β-agarase Aga 50D via sulfhydryl groups introduced at specific cysteine residues,enabling binding to the carrier.[Result ] The immobilization conditions were optimized as follows:reaction duration of 12 h,an enzyme loading level (enzyme-to-carrier mass ratio ) of 1∶50,and a crosslinker-to-carrier mass ratio of 10∶50.The immobilization efficiency ranged from 40% to 75%,with most mutants retaining the relative enzyme activity > 80%.Compared with the wild-type free enzyme,the site-directed immobilized mutant β-agarase Aga 50D exhibited …
Ultrasonic-Microwave Assisted Extraction Of Flavonoids From Citri Reticulatae Pericarpium And High-Purity Purification Of Hesperidin,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Ultrasonic-Microwave Assisted Extraction Of Flavonoids From Citri Reticulatae Pericarpium And High-Purity Purification Of Hesperidin, Weiben Ge, Yiheng Li, Hongxin Wang
Journal of Food Science and Biotechnology
[Objective ] To explore the application prospects of Citri Reticulatae Pericarpium in the food industry and efficiently extract the active component hesperidin,while developing an effective purification process to enhance the purity and functional value of the active ingredients.[Method ] Ultrasonic-microwave assisted extraction (U-MAE ) was employed to extract the hesperidin from Citri Reticulatae Pericarpium,followed by a three-stage purification system guided by dissolution characteristics involving alcohol removal by concentration,gradient ethanol washing,and pH adjustment to purify hesperidin.Single factor experiments and response surface methodology were used to optimize the extraction parameters.The antioxidant activity and α-glucosidase inhibitory activity of the final product were also …
Establishment Of A Hplc Method For Simultaneous Determination Of 11 Phenolic Acids In Germinated Black Wheat,
2025
Life Sciences Department , Yuncheng University , Yuncheng 044099 , China;College of Food Science and Engineering , Shanxi Agricultural University , Taigu 030801 , China;Shanxi Technology Innovation Center of High Value-Added Echelon Utilization of Premium Agro-Products , Yuncheng 044099 , China
Establishment Of A Hplc Method For Simultaneous Determination Of 11 Phenolic Acids In Germinated Black Wheat, Qian Li, Xiaodong Li, Ziyi Yang, Yueyao Zhang, Rui Liu, Zhanglong Yu, Yuanlin Sun
Journal of Food Science and Biotechnology
[Objective ] In order to study the effects of germination on the mass fractions of phenolic acids in black wheat,a method was established for simultaneous determination of 11 phenolic acids (gallic acid,protocatechuic acid,4-hydroxybenzoic acid,chlorogenic acid,vanillic acid,caffeic acid,syringic acid,p-coumaric acid,ferulic acid,sinapic acid,and salicylic acid ) in germinated black wheat based on high performance liquid chromatography (HPLC ).[Method ] The mobile phase composed of methanol (containing acetic acid at a volume fraction of 1%) and water (containing acetic acid at a volume fraction of 1%) was used for gradient elution at a flow rate of 1 mL/min,a detection wavelength of 280 nm,and …
Dual-Nozzle 3d Printing And Drying Characteristics Of A Hawthorn Snack Based On Dual-Material Combination Gel,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Dual-Nozzle 3d Printing And Drying Characteristics Of A Hawthorn Snack Based On Dual-Material Combination Gel, Aimiao Wang, Bo Wang, Yuchuan Wang, Jiguang Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims at solving the problems such as long drying time,single product shape,and poor 3D printing capacity (easy blockage and weak bracing ability ) in conventional processing of hawthorn snack products.[Method ] The low-sugar hawthorn jam and high-sugar banana jam were used as gel components.The 3D printing was carried out with the optimization of dual-material combination gel formula and the optimization of dual-nozzle 3D printing process.Furthermore,the drying characteristics of 3D printing were analyzed and compared with those of conventional dried hawthorn products.[Result ] The combination of hawthorn jam with the sugar mass fraction of 26% and banana …
Respiratory Characteristics Of Late-Maturing Sweet Cherry Cultivars And Their Influence By Temperature And Packaging Methods,
2025
Academy of Agriculture and Forestry Sciences , Qinghai University , Xining 810016 , China;Laboratory for Research and Utilization of Qinghai-Tibet Plateau Germplasm Resources , Xining 810016 , China;Key Laboratory of Qinghai-Tibetan Plateau Biotechnology (Qinghai University ), Ministry of Education , Xining 810016 , China;Key Laboratory of Agricultural Integrated Pest Management , Xining 810016 , China
Respiratory Characteristics Of Late-Maturing Sweet Cherry Cultivars And Their Influence By Temperature And Packaging Methods, Huanhuan Zhi, Yu Dong
Journal of Food Science and Biotechnology
[Objective ] This study aims to develop suitable packaging strategies for sweet cherry varieties with different respiratory characteristics.[Method ] The sweet cherry cultivars ‘Lapins’ and ‘Regina’ were put in closed systems with different O 2 volume fractions at 0,5,and 10 ℃.The changes in O 2 consumption rate,CO 2 consumption rate,and respiratory quotient were monitored.Additionally,the changes of gas components,quality,and physiological disorders were assessed for the two cultivars treated with polyethylene (PE) and modified atmosphere packaging (MAP ) after 4 weeks of storage at 0,5,and 10 ℃.[Result ] ‘Lapins’ had lower O 2 consumption rate and CO 2 consumption rate than ‘Regina’ …
Effects Of Ginger Protease On Beef Quality And Protein Degradation,
2025
School of Liquor and Food Engineering , Guizhou University , Guiyang 550025 , China
Effects Of Ginger Protease On Beef Quality And Protein Degradation, Xinghua Chen, Shuhong Liu, Huaisheng Zheng, Zhaobin Huang, Ping Hu
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of ginger protease on beef tenderness.[Method ] Ginger protease was extracted from mature ginger via ammonium sulfate precipitation.The fundamental properties of the ginger protease,including enzyme activity,protein mass fraction,optimal temperature and pH,and relative molecular mass,were evaluated.Furthermore,the effects of ginger protease on the physicochemical properties,sensory quality,microstructure,and protein degradation characteristics of beef were examined.[Result ] Ginger protease was mainly composed of two proteins with relative molecular masses of 15 293 and 34 191,with a total protein mass fraction of (574.60±0.25) mg/g,an enzyme activity of (31 787±307) U/g,an optimum temperature of 60 ℃,and an optimum …
Recombinant Pichia Pastoris Fermentation For Biosynthesis Of The Uv Resistant Compound Gadusol,
2025
Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Jiaxing Institute of Future Food , Jiaxing 314001 , China
Recombinant Pichia Pastoris Fermentation For Biosynthesis Of The Uv Resistant Compound Gadusol, Wenjie Zuo, Ping Zhang, Ruirui Xu, Leyi Zhang, Zhen Kang, Jianghua Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to employ synthetic biology and metabolic engineering techniques to engineer Pichia pastoris (P.pastoris ) for the efficient heterologous synthesis of the UV resistant compound gadusol.[Method ] On the basis of a bioinformatic analysis and codon optimization of the genes involved in the gadusol biosynthetic pathway in Danio rerio,the gadusol fermentation pathway was constructed in P.pastoris via strategies such as promoter engineering,metabolic engineering,and fermentation optimization.[Result ] A high-purity gadusol standard was prepared through optimized extraction and chromatographic separation.The gadusol yield in the recombinant strain P.pastoris HME reached 105 mg/L.By screening of gene expression promoters and optimization …
Effects Of Critical Environmental Factors On The Antimicrobial Activity Of Ε-Poly- L-Lysine,
2025
Institute of Microbiology , Guangdong Academy of Sciences , Guangzhou 510070 , China;Guangdong Detection Center of Microbiology , Guangzhou 510070 , China;State Key Laboratory of Applied Microbiology Southern China , Guangzhou 510070 , China;Guangdong Provincial Key Laboratory of Microbial Safety and Health , Guangzhou 510070 , China;National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food , Guangzhou 510070 , China
Effects Of Critical Environmental Factors On The Antimicrobial Activity Of Ε-Poly- L-Lysine, Zhenjie Liu, Peihao Huang, Shu Chen, Shun Rong, Feiting Peng, Ling Chen, Weipeng Guo
Journal of Food Science and Biotechnology
[Objective ] This study explored the effects of critical environmental factors [water activity (aw),pH,and temperature ] on the antimicrobial activity of ε-poly- L-lysine (ε-PL),aiming to provide a basis for the application of ε-PL.[Method ] With Staphylococcus aureus as an indicator strain,the micro-broth dilution and agar dilution methods as well as antimicrobial growth kinetics were utilized to determine the antimicrobial activity of ε-PL.The protein leakage assay was employed to evaluate the bacterial membrane disruption activity of ε-PL.Live/Dead fluorescence staining was performed to observe the bacterial membrane permeability.[Result ] The minimum inhibitory mass concentration (MIC ) of ε-PL against Staphylococcus aureus was …
Probiotic Properties Of Three Strains Of Lactobacillus And Their Application In Corn Husk Fermentation,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Probiotic Properties Of Three Strains Of Lactobacillus And Their Application In Corn Husk Fermentation, Bo Zhai, Quanxian Shu, Xiaoyan Yang, Yin Mao, Yu Deng, Yunying Zhao
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the utilization of maize resources and address the shortage of feed resources.[Method ] The intestinal colonization capacity,tolerance,and antibacterial properties of three Lactobacillus strains,Lactobacillus paracasei ZB- 1 (ZB-1),L.paracasei DY- 2 (DY-2),and L.rhamnosus DY- 4 (DY-4) were evaluated.The most suitable strain for fermenting cornhusks was identified,and its fermentation process was optimized.[Result ] ZB-1 exhibited higher intestinal colonization ability and antibacterial properties than DY- 2 and DY- 4.The antibacterial mechanism of ZB- 1 was likely driven by the action of organic acids in an acidic environment and certain protein-based antimicrobial substances.The fermentation conditions for ZB- 1 …
Diversity Of Fungi On The Surfaces Of Grapes And Raisins In Turpan , Xinjiang,
2025
College of Chemistry and Chemical Engineering , Xinjiang Normal University , Urumqi 830054 , China;Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Research Institute , Urumqi 830011 , China
Diversity Of Fungi On The Surfaces Of Grapes And Raisins In Turpan , Xinjiang, Lei Sun, Laifang Qi, Qiuyan Liang, Jinxuan Han, Tianli Fan, Yuhua Ma
Journal of Food Science and Biotechnology
[Objective ] This study aims to understand the potential harmful fungi that may affect the quality of raisins during the drying process of grapes from Turpan,Xinjiang and to explore the relationships between varieties and the fungal community structure and diversity on the surfaces of grapes and raisins.[Method ] Four popular grape varieties (‘Thompson Seedles s’,‘Boert e’,‘Xiangfe i’,and ‘Seedless Blac k’) in a grapery in Turpan were selected as research objects.The fungal diversity on the surfaces of grapes and raisins was analyzed via high-throughput sequencing.[Result ] The dominant fungal phyla on the surfaces of grapes and raisins were Ascomycota and Basidiomycota.The …
Regulatory Effects Of Hesperetin And Eriocitrin On Gut Microbiota In An In Vitro Simulation Model,
2025
School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Regulatory Effects Of Hesperetin And Eriocitrin On Gut Microbiota In An In Vitro Simulation Model, Zeren Cheng, Xia Meng, Kejing Tang, Ling Shen, Qing Gu, Ping Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to compare the effects of hesperetin and eriocitrin on the gut microbiota in different enterotypes.[Method ] An in vitro gut fermentation model was established,which was combined with 16S rRNA high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS ) technique to evaluate the regulatory effects of the two flavonoids on the gut microbiota in different enterotypes.[Result ] After fermentation,both flavonoids significantly reduced the Firmicutes-to-Bacteroidota ratio (F/B ratio ) in terms of relative abundance at the phylum level in enterotype Bacteroides and enterotype Prevotella.At the genus level,pro-inflammatory bacteria including Paeniclostridium and Sutterella showed decreased relative abundance in treatment …
Effects Of Ultrasound-Assisted Enzymatic Extraction On The Physical And Chemical Properties Of Tortoise Shell Collagen,
2025
School of Food and Biological Engineering , Hefei University of Technology , Hefei 230601 , China
Effects Of Ultrasound-Assisted Enzymatic Extraction On The Physical And Chemical Properties Of Tortoise Shell Collagen, Xiong He, Rongxing Li, Shaotong Jiang, Jianfeng Lu, Lin Lin
Journal of Food Science and Biotechnology
[Objective ] The effects of different extraction methods on the yield,purity,structural characteristics,and physicochemical properties of tortoise shell collagen were investigated.[Method ] The ultrasound-assisted enzymatic method,acid method,and enzymatic method were used to extract collagen from tortoise shell powder.[Result ] The yield and purity of tortoise shell collagen extracted by ultrasound-assisted enzymatic method were 2.29% and 85.65%,respectively,significantly higher than those extracted by acid method (0.78% and 73.15%) and enzymatic method (1.77% and 75.63%).Ultraviolet absorption spectroscopy,infrared absorption spectroscopy,circular dichroism spectroscopy,and electrophoresis profiles showed that collagens extracted from tortoise shell by three methods were all type I collagen and retained intact triple helix structures.X-ray …
Natural Pigment-Mediated Photodynamic Antimicrobial Technology : Mechanisms And Food Applications,
2025
School of Food and Bioengineering , Zhengzhou University of Light Industry , Zhengzhou 450001 , China;Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation , Nanyang Institute of Technology , Nanyang 473004 , China
Natural Pigment-Mediated Photodynamic Antimicrobial Technology : Mechanisms And Food Applications, Lei Zhao, Wenli Jiang, Qingshan Shen, Jingxuan Ke, Yanli Ma, Lifang Zhang
Journal of Food Science and Biotechnology
Photodynamic antimicrobial technology (PDT ),as a non-thermal physical bactericidal method,has received increasing attention due to its high efficiency,low residue,and lack of resistance induction.Natural pigments,as food-grade photosensitizers exhibit excellent optical responsiveness and biocompatibility.Studies have shown that curcumin,chlorophylls,and porphyrin-based pigments exhibit favorable photodynamic antimicrobial efficacy in various food matrices including fruits,vegetables,meat,and drinking water.The author summarizes the photodynamic antimicrobial mechanisms of representative natural pigments,and analyzes the effects of light parameters,photosensitizer concentrations,and food matrices on photodynamic antimicrobial efficacy.Furthermore,the recent progress of PDT applications in fruit and vegetable surface disinfection,drinking water sterilization,and smart packaging is reviewed,with a focus on the role of nanocarriers and composite …
Research Progress In Aflatoxin Degradation Technologies For Edible Oils,
2025
School of Public Health , Guangxi Medical University , Nanning 530021 , China
Research Progress In Aflatoxin Degradation Technologies For Edible Oils, Huishuai Li, Zhenning Li, Yu Xie, Yunfeng Zou, Shaojun Li
Journal of Food Science and Biotechnology
Aflatoxin (AF),a notorious contaminant,has been a persistent challenge to the safety of edible oils.Prolonged exposure to low doses or acute consumption of high amounts of AF may induce serious health issues.Currently,no method can completely eliminate AF from edible oils.Therefore,it is crucial to explore strategies for reducing AF levels in edible oils.The measures to degrade AF from edible oils were reviewed,mainly including physical methods (such as high-temperature treatment,ray irradiation,and adsorption ),chemical methods (such as ammonia fumigation and ozone oxidation ),and biological methods (such as microbial degradation ).In addition,this paper analyzed and discussed the principles,advantages,shortcomings,and safety of various degradation methods.Furthermore,it made an …
Optimization Of Fermentation Conditions For The Removal Of Zearalenone From Corn Gluten Feed By Aspergillus Niger Fs10,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China ;Institute of Future Food Technology , JITRI , Wuxi 214200 , China
Optimization Of Fermentation Conditions For The Removal Of Zearalenone From Corn Gluten Feed By Aspergillus Niger Fs10, Mengying Wu, Yang Yang, Jinyao Wang, Yongli Ye, Li’Na Sheng, Jian Ji, Xiulan Sun
Journal of Food Science and Biotechnology
[Objective ] This study aims to determine the optimal solid-state fermentation conditions for the removal of zearalenone (ZEN ) from corn gluten feed by Aspergillus niger (A.niger ) FS10.[Method ] With the removal rate of ZEN as the indicator,single-factor experiments and response surface method were employed to optimize the key fermentation parameters (inoculation volume-to-mass ratio,fermentation temperature,fermentation duration,and solid-to-liquid ratio ).Furthermore,changes in mass fractions of key nutritional components in corn gluten feed before and after solid-state fermentation under the optimal conditions were analyzed.[Result ] The optimal fermentation conditions were established as follows:an inoculation volume-to-mass ratio of 120 μL∶1 g,a fermentation temperature …
Fermentation Characteristics Of Biofilm-Forming Lactic Acid Bacteria In Sausages,
2025
College of Food Science and Technology , Nanjing Agricultural University , Nanjing 211800 , China
Fermentation Characteristics Of Biofilm-Forming Lactic Acid Bacteria In Sausages, Jie Shi, Yue Sun, Ruidian Zhang, Huifen He, Tenglong Miao, Qiuqin Zhang
Journal of Food Science and Biotechnology
[Objective ] To address the challenge of controlling harmful bacteria during the fermentation process of sausages,this study optimized the biofilm formation conditions of Lactiplantibacillus plantarum (L.plantarum ) JB1 to enhance its competitive advantage in the low-temperature fermentation environment of sausages.Furthermore,this study systematically evaluated the regulatory role of the biofilm-forming L.plantarum JB1 in the quality and safety of sausages,aiming to provide process parameters and a theoretical basis for the development of high-stability probiotic formulations.[Method ] Single-factor and orthogonal experiments were conducted to quantify the effects of the interactions between temperature,pH,carbon source,nitrogen source,metal ions,and buffer salts on the biofilm-forming L.plantarum JB1.A sausage …
Effect Of Caffeine Energy Blending Formula On Mood,
2025
College of Food Science and Engineering , Northwest A&F University , Yangling 712100 , China
Effect Of Caffeine Energy Blending Formula On Mood, Xiaorui Wang, Mengzhen Jia, Tian Yuan, Zhigang Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effect of the energy blending formula with caffeine as the major component on mood improvement.[Method ] A total of 240 participants were assigned into an experimental group and a control group,with 120 participants in each group.The experimental group consumed the drink containing energy blending formula with caffeine as the major component,and the control group consumed the same flavor drink without this energy blending formula.The Profile of Mood States (POMS ) scale was used to assess mood states.Participants completed the scale at four time points:before consumption and at 30,60,and 90 min post-consumption.[Result ] …
Effects Of Cooking Processes On Texture And Umami Retention Of Ruditapes Philippinarum,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China;Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province , Wuxi 214122 , China
Effects Of Cooking Processes On Texture And Umami Retention Of Ruditapes Philippinarum, Peipei Yu, Shuhan Yang, Qixing Jiang, Pei Gao, Xiaojuan Yu, Pengyi Li, Yi Sun, Chunye Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the quality and umami changes of Ruditapes philippinarum during cooking.[Method ] The cooking loss rate,texture,amino acid nitrogen,free amino acids,flavor nucleotides,glycogen,and sensory quality of Ruditapes philippinarum were measured after cooking (at 85,90,95,and 100 ℃ for 1,2,3,4,and 5 min ).[Result ] At the same cooking temperature,the cooking loss rate and hardness of Ruditapes philippinarum increased with the increase in cooking time.At the cooking temperatures of 90 and 95 ℃,the mass fractions of amino acid nitrogen in Ruditapes philippinarum generally presented a decreasing trend as the cooking time increased within 1 to 5 min.At the same …
Effects Of Cutting Size And Steaming/Boiling Time On The Quality Of Colored Mashed Potatoes,
2025
Academy of Agriculture and Forestry Sciences , Qinghai University , Xining 810016 , China
Effects Of Cutting Size And Steaming/Boiling Time On The Quality Of Colored Mashed Potatoes, Yuqing Che, Chi Chi, Yu Dong, Tiancang Na
Journal of Food Science and Biotechnology
[Objective ] This study explored the effects of steaming/boiling time and cutting size on the color,texture,starch structure,and anthocyanin mass fraction of red and purple potatoes before and after cooking,aiming to provide a theoretical basis for the processing of colored mashed potatoes.[Method ] Red and purple potatoes were taken as the research objects.They were cut into three different sizes (30 mm× 30 mm× 10 mm,20 mm× 20 mm× 10 mm,and 16 mm× 16 mm× 10 mm ) and steamed or boiled for five time periods (0,3,5,7,and 10 min ).The color parameters (including surface brightness L*,red-green value a*,and yellow-blue value b*),texture properties …
Advances In The Mechanisms Of The Effects Of Pseudomonas Spp. Proteases On Gelatinization Of Dairy Products And Regulation Strategies,
2025
Food Science College , Shenyang Agricultural University , Shenyang 110866 , China;Liaoning Food Fermentation Technology Engineering Research Center , Shenyang 110866 , China;Shenyang Key Laboratory of Microbial Fermentation Technology Innovation , Shenyang 110866 , China
Advances In The Mechanisms Of The Effects Of Pseudomonas Spp. Proteases On Gelatinization Of Dairy Products And Regulation Strategies, Shengjia Liu, Kairu He, Hui Li, Yongqiang Li, Xinyi Li, Rina Wu, Junrui Wu, Sanjun Zhao
Journal of Food Science and Biotechnology
Gelatinization of dairy products has long been a common problem caused by heat-resistant protease contamination.Despite the high-temperature sterilization process,heat-resistant proteases secreted by Pseudomonas spp.can survive under extreme conditions.These enzymes can break down the peptide bonds of proteins in milk,resulting in the bitter taste and gelatinization of milk,which seriously affects the quality and shelf-life of dairy products.In view of the complex gelatinization mechanisms of dairy products and the lack of in-depth investigation,the author systematically outlined the characteristics,enzyme activity detection methods,and influencing factors of Pseudomonas spp.proteases,delved into the mechanisms underpinning the protease-induced gelatinization of dairy products,and summarized the control measures and novel …
