Food Microbiology Commons^™

Feed Supplementation With Natural Extracts Of Cranberry And Its Efficacy On Campylobacter Colonization In Poultry, Ann Woo-Ming

Graduate Theses and Dissertations

Campylobacter spp. has been identified as one of the leading causative agents of food borne diarrheal illness. Epidemiological evidence has shown that poultry is the main source for human infection. Poultry are asymptomatic carriers of Campylobacter within their gastrointestinal tract, with colonization levels reaching 106-108 cfu/g cecal content. Surveys of domestic poultry flocks have estimated that approximately 90% of flocks are positive for Campylobacter colonization. Risk assessment studies have determined that by reducing levels of Campylobacter colonization during rearing, incidences of human infection will be significantly reduced. Currently there are no consistently effective treatments to eliminate Campylobacter from poultry flocks. …

Role Of Lactic Acid Bacteria As A Bio-Sanitizer To Prevent Attachment Of Listeria Monocytogenes On Deli Slicer Contact Surfaces, And The Influence Of Listeria Innocua On The Attachment Of L. Monocytogenes On Selected Materials Located In The Food-Processing Environment, Jean Baptiste Ndahetuye

Graduate Theses and Dissertations

Listeria monocytogenes an important foodborne pathogen that continues to be a serious problem for the food industry. This pathogen contaminates food primarily during post-harvest in the food-processing environment. Its ecology in the food-processing environment is not well understood but previous research has demonstrated the ability of L. monocytogenes to survive on food contact surfaces after cleaning and disinfection. The current study explored the attachment of three lactic acid bacteria (LAB) strains, Lactobacillus animalis , Lb. amylovorus and Pediococcus acidilactici , their combination in a cocktail, and their influence to prevent the attachment of L. monocytogenes at room temperature by examining …

Sanitation Assessment Of Food Contact Surfaces And Lethality Of Moist Heat And A Disinfectant Against Listeria Strains Inoculated On Deli Slicer Components, Sabelo Muzikayise Masuku

Graduate Theses and Dissertations

The overall objectives of this study were to: evaluate the efficacy of different cleaning cloth types and cloth-disinfectant combinations in reducing food contact surface contamination to acceptable levels; determine the optimum moist heat and moist heat + sanitizer treatments that can significantly reduce the number of Listeria strains on deli slicer components; and investigate if the moist heat treatment used in this study induced the viable-but-non-culturable (VBNC) state in Listeria cells. The efficacy of wiping cloths was measured using ATP-bioluminescence and total plate count methods using four different wiping cloths and silver dihydrogen citrate sanitizer on food contact surfaces. The …

Validation Of Thermal Processing To Control Salmonella Spp. And Clostridium Perfringens During Prime Rib Preparation From Intact And Non-Intact Meat, Maria A. Calle

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Beef prime rib is a delicacy cooked at low temperatures for long period and extended hot holding prior to consumption. Due to the potential survival and outgrowth of foodborne pathogens, this process may pose a public health risk. Considering an increase in the availability of non-intact beef, the microbial safety of the preparation of prime rib from intact and mechanically tenderized beef was evaluated.

In the first study, lethality of the prime rib (from intact and non-intact, blade tenderized beef) cooking process to Salmonella spp. was evaluated. Boneless beef rib eye was surface inoculated with a five-strain cocktail of Salmonella …

Response Surface Methodology Approach For The Optimization Of Lab Fermentation Using Vegetable Based Substrate, Amit Jaiswal, Nissreen Abu-Ghannam, Juan Valverde

Conference papers

In the present studies, an attempt was made to optimize the conditions for the lactic acid bacteria (LAB) fermentation using Irish York cabbage (Brassica oleracea var. capitata alba subvar. conica) as a substrate. Prior to fermentation with Lactobacillus plantarum, York cabbage was blanched at 95°C for 12 min to inactivate surface microflora. To achieve an optimal fermentation condition which would result in higher release of phytochemicals and antioxidant capacity in the broth, Box-Behnken design integrating a desirability approach was used. The optimized factors (fermentation time 36h, solid/liquid ratio 0.25 g/ml and agitation rate 100 rpm) were used for …

Delicious-Ology: The Science Of Delicious Food, Sasha Yan

Undergraduate Theses—Unrestricted

You can read this book two ways. If you want to “just cook,” flip to the recipe index, pick a recipe, and skip straight to that page. The surrounding text will explain some aspects of the science behind the recipe. While the recipes in this book are chosen to complement and provide examples of the science, they’re also recipes that are fantastic in and of themselves.

Detection And Identification Of Salmonella Enterica, Escherichia Coli, And Shigella Spp. Via Pcr-Esi-Ms: Isolate Testing And Analysis Of Food Samples, Sarah E. Pierce, Rosalee S. Hellberg, Chorng-Ming Cheng, Kai-Shun Chen, Donna M. Williams-Hill, William B. Martin, Marc W. Allard

Food Science Faculty Articles and Research

An assay to identify the common food-borne pathogens Salmonella, Escherichia coli, Shigella, and Listeria monocytogenes was developed in collaboration with Ibis Biosciences (a division of Abbott Molecular) for the Plex-ID biosensor system, a platform that uses electrospray ionization mass spectroscopy (ESI-MS) to detect the base composition of short PCR amplicons. The new food-borne pathogen (FBP) plate has been experimentally designed using four gene segments for a total of eight amplicon targets. Initial work built a DNA base count database that contains more than 140 Salmonella enterica, 139 E. coli, 11 Shigella, and 36 Listeria patterns and 18 other Enterobacteriaceae organisms. …

Antimicrobial Efficacy Of Edible Soy Protein Isolate Films And Coatings Incorporated With Hop Ethanol Extract And The Influence On Shelf-Life And Sensory Attributes Of Bologna, Jamie R. G. Skudlarek

Theses and Dissertations--Animal and Food Sciences

There is demand for improved security of refrigerated ready-to-eat meats. Antimicrobial edible films and coatings could function as an added barrier against post-processing contamination. Hops and hop extracts are known for their antimicrobial efficacy which is attributed to key antimicrobial components including humulones, lupulones, xanthohumol and various terpenoids. Yet, hop ethanol extract has not been studied as an antimicrobial to incorporate into edible protein films and/or coatings. The overall objective of this research was to evaluate hop ethanol extract as an antimicrobial agent incorporated into edible soy protein isolate (SPI) films and coatings, and the influence on the shelf-life and …

Recent Advances In The Application Of Non Thermal Methods For The Prevention Of Salmonella In Foods, Shilpi Gupta, Nissreen Abu-Ghannam

Books/Book Chapters/ Proceedings

Food-borne illness as a result of consumption of foods contaminated with pathogenic bacteria is a world-wide concern. The presence and subsequent growth of micro-organisms in food in addition to improper storage not only results in spoilage but also in a reduction of food quality. The microbiological safety in ready to eat products is a cause of big concern not only for the consumers and food industries but also for the regulatory agencies. The number of documented outbreaks of foodborne diseases has increased in the last decade with Salmonella spp., Listeria monocytogenes and Escherichia coli being responsible for the largest number …

Antimicrobial, Antioxidant And Free Radical-Scavenging Capacity Of Brown Seaweed Himanthalia Elongata From Western Coast Of Ireland, Gaurav Rajauria, Amit Jaiswal, Nissreen Abu-Ghannam, Shilpi Gupta

Articles

Water,methanol and mixtures (20–80%) thereof have been used for the extraction of phenolic compounds from Irish brown seaweed Himanthalia elongata for studying its antimicrobial and antioxidant properties.The 60%methanolic extract exhibited significantly (P < 0.05) the highest value of yield (6.8 6 0.24%), total phenol (286.0 6 4.61 mg gallic acid equivalents/g), flavonoid (109.8 6 2.68 mg quercetin equivalents/g) and condensed tannin content (35.6 6 1.03 mgcatechin equivalents/ g). Antimicrobial activity of 60% methanolic extract tested from disc diffusion and broth dilution methods was effective against various food spoilage and pathogenic bacteria studied.The same extract exhibited statistically highest reducing power and antioxidant capacity againstDPPHradical,metal ions, lipid peroxides and hydrogen peroxide radicals. The UV-visible spectroscopy showed absorption maxima at 205 and 260 nm and the presence of hydroxyl group (3,431 cm-1) and an aromatic ring (around 1,465, 1,505 and 1,624 cm-1) in Fourier Transform Infrared spectroscopy, suggested the presence of phenolic compounds in the extract.

Evaluation Of Novel Low Molecular Weight Antimicrobial Compounds Produced By Lactic Acid Bacteria., Jiahui Leona Guo

Theses

Fungal food spoilage plays a pivotal role in the deterioration of food and feed systems and some fungi are also able to produce compounds toxic for humans and animals. The mycotoxins produced by fungi can cause serious health hazards and include toxins which are cancerogenic, immunotoxic, teratogenic, neurotoxic, nephrotoxic or hepatotoxic effects and Kashin-Beck disease. In addition to this, fungal spoilage/pathogens cause losses of marketable quality and hygiene of foodstuffs, resulting in major economic problem throughout the world. Nowadays, food spoilage can be prevented using physical and chemical methods, but no efficient strategy has been proposed so far to reduce …

Optimization Of Lactic Acid Fermentation Of York Cabbage For The Development Of Potential Probiotic Products, Amit Jaiswal, Shilpi Gupta, Nissreen Abu-Ghannam

Articles

In the present study, York cabbage was used as the sole substrate for the lactic acid (LA) fermentation with Lactobacillus plantarum. York cabbage was blanched at 95°C for 12min to inactivate surface microflora prior to fermentation. To achieve an optimal fermentation condition which would result in higher release of phytochemicals and antioxidant (AO) capacity in the broth, Box-Behnken design integrating a desirability approach was used. A second-order polynomial equation was developed indicating the effect of solute/liquid ratio, agitation rate and fermentation time on desired parameters. The optimized factors were used for fermenting York cabbage to obtain maximum bacterial growth, …

Studies On The Adherence Properties Of Plant Lectins And Bacterial Adhesins And Their Inhibition By Prebiotic Oligosaccharides And Bovine Colostrum Fractions, Maria X. Maldonado-Gomez

Department of Food Science and Technology: Dissertations, Theses, and Student Research

To initiate colonization and infection, most microbial pathogens must first recognize and adhere to cells in the host tissues. Adherence inhibition, therefore, can be used as a strategy for preventing infectious disease. In this research, we developed a simplified model using plant lectins and HEp-2 tissue culture cells to simulate bacterial adhesins-receptors interactions. The effect of lectin ligands and two prebiotic carbohydrates, galactooligosaccharide (GOS) and polydextrose (PDX) was measured with this approach. Lectins that were able to bind to the target cells were inhibited in the presence of the cognate ligands and at least one of the prebiotic carbohydrates. The …

Effect Of Sodium Nitrite, Sodium Erythorbate And Organic Acid Salts On Germination And Outgrowth Of Clostridium Perfringens Spores In Ham During Abusive Cooling, Mauricio A. Redondo

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The effect of sodium nitrite (NaNO₂), sodium erythorbate, sodium chloride (NaCl) and organic acid salts (potassium lactate and sodium diacetate) on C. perfringens spore germination and outgrowth in ham was evaluated. The research was divided in two parts. The first experiment consisted of potential C. perfringens spore germination and outgrowth in ham containing combinations of NaNO₂ (0, 50, 100, 150 or 200 ppm) and sodium erythorbate (0 or 557 ppm) during cooling. This experiment included the evaluation of residual nitrite levels as affected by temperature (heat shock at 75°C for 20 min and subsequent cooling from 54.4°C …

Investigation Of In-Package Ozonation: The Effectiveness Of Ozone To Inactive Salmonella Enteritidis On Raw, Shell Eggs, Austin Donner

The Journal of Purdue Undergraduate Research

Food production, handling, and distribution practices pose a constant threat to the quality and safety of food products. The objective of this research is to evaluate an innovative in-package ozonation process to reduce Salmonella enteritidis on raw, shell eggs. Previous research has shown that in-package ozonation eliminates contaminants from outside sources, reduces pathogens, and extends shelf life. In this study, raw, shell eggs were inoculated with Salmonella enteritidis and exposed to ozonation treatment. Microbial recoveries were then tested to determine bacterial reductions. Measurements included: relative humidity (34 percent at 5^oC), surface temperatures (^oC), ozone concentrations, bacterial …

Emerging Dynamics Of Human Campylobacteriosis In Southern Ireland, Susan Bullman, Daniel Corcoran, James O'Leary, Derry O'Hare, Brigid Lucey, Roy D. Sleator

Department of Biological Sciences Publications

Infections with Campylobacter spp. pose a significant health burden worldwide. The significance of Campylobacter jejuni/Campylobacter coli infection is well appreciated but the contribution of non-C. jejuni/C. coli spp. to human gastroenteritis is largely unknown. In this study, we employed a two-tiered molecular study on 7194 patient faecal samples received by the Microbiology Department in Cork University Hospital during 2009. The first step, using EntericBio® (Serosep), a multiplex PCR system, detected Campylobacter to the genus level. The second step, utilizing Campylobacter species-specific PCR identified to the species level. A total of 340 samples were confirmed as Campylobacter genus positive, 329 of …

The Antimicrobial Activity Of Cold Pressed Terpeneless Valencia Orange Oil At Cold Temperatures, Sean Jeffrey Pendleton

Graduate Theses and Dissertations

This research was undertaken to identify an antimicrobial for use during chilling in beef processing, including periods of temperature abuse, as well as to evaluate membrane filtration as means to remove essential oils from bacteria to prevent further antibacterial action. Cold pressed terpeneless Valencia orange oil was examined in combination with various temperatures (37°C, 10°C, and 4°C) to determine its antimicrobial activity against various strains of bacteria. The strains were tested using a ninety-six well microtiter plate method, with or without membrane filtration. The compound 2, 3, 5-triphenyl tetrazolium chloride was used as a growth indicator. Serial, two fold dilutions …

Optimisation Of Accelerated Solvent Extraction Of Antioxidant Compounds From Rosemary (Rosmarinus Officinalis L.), Marjoram (Origanum Majorana L.) And Oregano (Origanum Vulgare L.) Using Response Surface Methodology, M. Hossain, Catherine Barry-Ryan, Ana Belen Martin-Diana, N. Brunton

Articles

The present study optimised the accelerated solvent extraction (ASE) conditions (Dionex ASE® 200, USA) to maximise the antioxidant capacity of the extracts from three spices of Lamiaceae family; rosemary, oregano and marjoram. Optimised conditions with regard to extraction temperature (66–129 °C) and solvent concentration (32–88% methanol) were identified using response surface methodology (RSM). For all three spices results showed that 129 °C was the optimum temperature in order to obtain extracts with high antioxidant activity. Optimal methanol concentrations with respect to the antioxidant activity of rosemary and marjoram extracts were 56% and 57% respectively. Oregano showed a different response to …

Hyperspectral Imaging For The Detection Of Microbial Spoilage Of Mushrooms, Edurne Gaston, Jesus Maria Frias, Patrick Cullen, Colm O’Donnell, Aoife Gowen

Conference papers

Brown blotch, caused by pathogenic Pseudomonas tolaasii, is the most problematic bacterial disease in Agaricus bisporus mushrooms; it reduces their consumer appeal in the market place, thus generating important economical losses worldwide. The mushroom industry is in need of fast and accurate evaluation methodologies to ensure that only high quality produce reaches the market. Hyperspectral imaging (HSI) is a non-destructive technique that combines imaging and spectroscopy to obtain spatial and spectral information from an object. The aim of this study was to investigate the potential of Vis-NIR HSI to identify microbiological damage in mushrooms and to discriminate it from …

Analysis Of Rice Blast Resistance Genes In The National Small Grains Collection (Nsgc), Moytri Roychowdhury

Graduate Theses and Dissertations

Identification of blast resistance genes in rice germplasm is one of the most important activities of rice breeding programs in worldwide. The objective of this research was to characterize two major blast resistance genes, Pi-z and Pi-b, in selected rice germplasm. A simple sequence repeat DNA marker, AP5659-1 linked to the Pi-z gene, and a Pib dominant marker derived from the Pi-b gene were first used to screen a rice core collection consisting of 1700 accessions estimated to represent 70% of the genetic diversity of rice in the US National Small Grains Collection. There were 131 rice germplasm accessions with …