Isolation And Partial Characterization Of Bioactive Fucoxanthin From Himanthalia Elongata Brown Seaweed: A Tlc-Based Approach,
2013
Technological University Dublin
Isolation And Partial Characterization Of Bioactive Fucoxanthin From Himanthalia Elongata Brown Seaweed: A Tlc-Based Approach, Gaurav Rajauria, Nissreen Abu-Ghannam
Articles
Seaweeds are important sources of carotenoids, and numerous studies have shown the beneficial effects of these pigments on human health. In the present study, Himanthalia elongata brown seaweed was extracted with a mixture of low polarity solvents, and the crude extract was separated using analytical thin-layer chromatography (TLC).The separated compounds were tested for their potential antioxidant capacity and antimicrobial activity against Listeria monocytogenes bacteria using TLC bioautography approach. For bio-autography, the coloured band on TLC chromatogram was visualized after spraying with DPPH and triphenyltetrazolium chloride reagents which screen antioxidant and antimicrobial compounds, respectively, and only one active compound was screened …
Food Defense Among Meat Processing And Food Service Establishments In Kentucky,
2013
Western Kentucky University
Food Defense Among Meat Processing And Food Service Establishments In Kentucky, Morgan Webb-Yeates
Masters Theses & Specialist Projects
Agroterrorism is the deliberate introduction of a plant or animal disease with the
goal of causing fear, economic instability, illness, or death. After the 2002 terrorist
attacks on the World Trade Center, the security of the food supply is of increasing
concern to the United States. A major incidence of agroterrorism or food tampering would have far reaching impacts on the economy and public health. The first objective of this project was to determine knowledge and concern of agroterrorism in meat processing facilities in Kentucky, and to determine knowledge and concern of food tampering and food defense in food service …
Physio-Chemical And Sensory Properties Of A Nutrient-Fortified Extruded Product,
2013
University of Arkansas, Fayetteville
Physio-Chemical And Sensory Properties Of A Nutrient-Fortified Extruded Product, Tajudini Lassissi Akande
Graduate Theses and Dissertations
Protein malnutrition is responsible for half the deaths of children under the age of five each year in developing countries. More than 4%, 26%, and 70% of children with protein malnutrition live in Latin America/the Caribbean, Africa, and Asia, respectively. The objective of this research was to develop a novel snack made with millet, black eye bean (from Niger Republic), and rice flour fortified with soybean meal protein using extrusion technology. Proximate analysis of the four flours was carried out to determine their chemical composition. Central composite design (CCD) was used to obtain best extrusion conditions to develop a protein-enriched …
Development Of A Predictive Modeling System For Validation Of The Cumulative Mirobial Inactivation Of The Salmonellae In Pepperoni Utilizing A Non-Pathogenic Surrogate Microorganism (Enterococcus Faecalis),
2013
University of Arkansas, Fayetteville
Development Of A Predictive Modeling System For Validation Of The Cumulative Mirobial Inactivation Of The Salmonellae In Pepperoni Utilizing A Non-Pathogenic Surrogate Microorganism (Enterococcus Faecalis), Scott Donald Stillwell
Graduate Theses and Dissertations
Salmonellosis is the most frequently occurring bacterial foodborne illness in the United States and the human case rate has not improved for the past two decades. The federal agency responsible for oversight of meat and poultry processors has announced the intent to extend existing pathogen reduction performance standards for Salmonella spp. to cover all classes of products including fermented sausages that are currently produced under HACCP plans that are validated for control of Escherichia coli O157:H7. The proposed regulatory modifications will require processors to revalidate HACCP plan controls to achieve either a 6.5 or a 7.0 log10 inactivation of the …
A Single Point Mutation In The Listerial Betl Sigma(A)-Dependent Promoter Leads To Improved Osmo- And Chill-Tolerance And A Morphological Shift At Elevated Osmolarity,
2013
Alimentary Pharmabiotic Centre; University College Cork; Cork, Ireland; Department of Biological Sciences; Cork Institute of Technology; Cork, Ireland.
A Single Point Mutation In The Listerial Betl Sigma(A)-Dependent Promoter Leads To Improved Osmo- And Chill-Tolerance And A Morphological Shift At Elevated Osmolarity, Ronald F. Hoffman, Susan Mclernon, Audrey Feeney, Colin Hill, Roy D. Sleator
Department of Biological Sciences Publications
Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported “twisted” cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy …
Aqueous Extract Of Nigella Sativa Seeds Suppresses Testicular Steroidogenesis In Mice Leydig Cells In Vitro.,
2013
Aga Khan University
Aqueous Extract Of Nigella Sativa Seeds Suppresses Testicular Steroidogenesis In Mice Leydig Cells In Vitro., 5sheikh A. Saeed, Naheed Anwar, Qaiser Jabeen, Anwar Hassan Gilani
Department of Biological & Biomedical Sciences
10ABSTRACT 11Nigella sativa (black seed) is an important medicinal herb with folkloric use in wide range of diseases. It is 12well studied for its biological activities. However, there is limited information regarding its effect on the 13male reproductive system. This study describes the effect of the aqueous extract of N. sativa (NSE) on 14 testicular steroidogenesis from mice Leydig cells in vitro. Mice testicular cells were incubated in a media 15 containing either no treatment or NSE or LH alone or combination of LH and NSE. Incubations were 16 carried out for three hours in a shaking water bath at …
Utilisation Of Spent Dairy Yeast Kluyveromyces Marxianus For The Production Of Flavoured Yeast Extracts.,
2013
Department of Biological and Pharmaceutical Sciences, Institute of Technology, Tralee, Kerry, Ireland
Utilisation Of Spent Dairy Yeast Kluyveromyces Marxianus For The Production Of Flavoured Yeast Extracts., Brendan Mccarra
Theses
Spent dairy yeast Kluyveromyces marxianus (SDYK), a by-product of whey to ethanol fermentations, was investigated as a natural biomass source for the production of alternative flavoured yeast extracts. SDYK which was characterised by a high protein content (55.3% (w/w) ± 0.8) and comparable levels of flavor enhancing amino acids to that of S. cerevisiae was prepared for induced autolysis at different pH values in the presence of inductors sodium chloride and the proteolytic enzyme papain. An induced autolysis process was developed for the production of flavoured yeast extracts in which pH and autolytic treatment applied were found to have a …
Antilisterial Characteristics Of Volatile Essential Oils,
2013
University of Kentucky
Antilisterial Characteristics Of Volatile Essential Oils, Leeann L. Slaughter
Theses and Dissertations--Animal and Food Sciences
This study explored the in vitro and in situ antilisterial inhibitory activity of 16 essential oils during indirect exposure: Spanish Basil oil (Ocimum basilicum), Bay oil (Pimenta racemosa), Italian Bergamot oil (Citrus bergamia), Roman Chamomile oil (Anthemis nobilis), Sir Lanka Cinnamon oil (Cinnamomum zeylanicum), Citral, Clove Bud oil (Syzygium aromaticum), Cumin Seed oil (cuminum cyminum), Eucalyptus oil (Eucalyptus globulus), Eugenol, Geranium extract (Pelargonium graveolens), Marjoram oil (Origanum majorana), Neroli extract (Citrus aurantium), Peppermint oil (Mentha piperita L.), …
Innovative Food Product Development Using Molecular Gastronomy: A Focus On Flavour And Sensory Evaluation,
2013
Technological University Dublin
Innovative Food Product Development Using Molecular Gastronomy: A Focus On Flavour And Sensory Evaluation, Mark Traynor
Doctoral
The primary goal of this research was to develop novel ice cream products using the principles of molecular gastronomy. An ice cream model system (emulsion) was developed, in which the effects of ingredient levels on stability and formation were investigated and optimised using Response Surface Methodology (RSM). Two characteristic volatiles of banana (isoamyl acetate and furfuryl acetate) were added to the optimised emulsion, and their headspace emission was quantified using Solid Phase Microextraction with Gas Chromatography Mass Spectrometry. RSM was used to investigate the main and interactive effects of ingredient, salt and pH levels on the headspace emission of these …
Optimization Of Fermentation Conditions For The Utilization Of Brewing Waste To Develop A Nutraceutical Rich Liquid Product,
2013
Technological University Dublin
Optimization Of Fermentation Conditions For The Utilization Of Brewing Waste To Develop A Nutraceutical Rich Liquid Product, Shilpi Gupta, Amit Jaiswal, Nissreen Abu-Ghannam
Articles
Utilization of brewers’ spent grain (BSG), for the development of a fermented liquid product rich in value-added phenolic compounds was investigated. Changes in and liberation of phenolic compounds and antioxidant activity during fermentation of BSG was studied. The effect of various particle size (PS), solid liquid (SL) ratio, fermentation time and rotation speed was optimized using response surface methodology (RSM) for the purpose of improving bacterial growth and the enhancement in the release of polyphenolic compounds. Contour maps generated using the response surface equation showed that the experimental variables significantly affected the response. A production of 10.4 log cfu/ml, 2.95 …
The Effect Of Non-Thermal Processing Technologies On Microbial Inactivation: An Investigation Into Sub-Lethal Injury Of Escherichia Coli And Pseudomonas Fluorescens,
2013
Technological University Dublin
The Effect Of Non-Thermal Processing Technologies On Microbial Inactivation: An Investigation Into Sub-Lethal Injury Of Escherichia Coli And Pseudomonas Fluorescens, Rachel Halpin, L. Duffy, O. Cregenzán-Alberti,, J.G Lyng, F. Noci
Articles
In recent years, there has been an increased interest in food processing technologies that could lessen the thermal impact on food products. In the present study, thermosonication (TS) and pulsed electric fields (PEF), applied individually or in combination (TS/PEF), were investigated to determine their effects on inactivation and sub-lethal injury of Pseudomonas fluorescens and Escherichia coli. TS was applied at a low (L) and high (H) wave amplitude (18.6 μm and 27.9 μm, respectively), while PEF was applied at a low and high electrical field strength (29 kV cm−1 and 32 kV cm−1, respectively). In addition, …
Cloning, Expression, And Characterization Of Ara H 3, A Major Peanut Allergen,
2012
University of New Orleans
Cloning, Expression, And Characterization Of Ara H 3, A Major Peanut Allergen, Cathryn E. Garvey
LSU New Orleans Theses and Dissertations
Abstract There are eight foods that contribute to food allergies in the western world and peanut is the most common. Currently, there are no medical treatments that can cure an individual of food allergy, so avoidance of the allergic food is the only option. In the United States, there are three immunodominant allergic proteins accountable for patient sensitization to peanut, Arachis hypogea 1, 2, and 3 (Ara h 1, Ara h 2, Ara h 3). Therefore, research into why peanuts are more allergic than other foods that have homologous proteins is critical and may be obtained by studying the structural …
Evidence Of Transmission Of Escherichia Coli O157:H7 To The Tissues Or Phyllo-Plane Of Wheat, From Contaminated Soil, Seeds Or Water,
2012
University of Nebraska-Lincoln
Evidence Of Transmission Of Escherichia Coli O157:H7 To The Tissues Or Phyllo-Plane Of Wheat, From Contaminated Soil, Seeds Or Water, Bismarck Antonio Martinez
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Escherichia coli O157:H7 is a human pathogen that can cause a wide spectrum of disease symptoms, such as bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). Escherichia coli O157:H7 illness are mainly associated with undercooked beef; however, in recent years outbreaks have been linked to fresh produce such as spinach, lettuce, and sprouts. In 2009, flour was implicated as a contamination source in the consumption of raw cookie dough resulting in 77 illness-cases. The objective of this research was to determine the possible route of transmission of E. coli O157:H7 into the phyllo-plane of wheat using contaminated seed, soil …
Analysis Of Microbial Diversity By Amplicon Pyrosequencing,
2012
University of Nebraska-Lincoln
Analysis Of Microbial Diversity By Amplicon Pyrosequencing, Ryan Legge
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Microorganisms numerically dominate terrestrial biodiversity, and play important biochemical and geochemical roles in the environments they inhabit. To understand structure and function of complex ecosystems, it is essential to identify primary drivers of microbial diversity and community structure. Historically, the study of microbial ecology was reductive, limited to microbes able to be cultured and enumerated. Microbes meeting this criterion were thought to comprise the dominating members of the environments they were isolated from, however, estimates suggesting up to 99% of the endogenous species are uncultivable with existing methodologies; a concept that reflects experimental failure, rather than a verifiable conclusion. Therefore …
The Trade And Welfare Impacts Of Australian Quarantine Policies: The Case Of Pigmeat,
2012
Iowa State University
The Trade And Welfare Impacts Of Australian Quarantine Policies: The Case Of Pigmeat, John C. Beghin, Mark Melatos
Department of Agricultural Economics: Faculty Publications
We analyze the trade and welfare impact of quarantine measures imposed by Australia on imports of pigmeat. In particular, we account for changes to Australia’s pigmeat quarantine policy over time, including those changes related to the recent resolution of a WTO dispute between Australia and the European Union. Using a random utility model and applying it to corner solutions in import decisions, tariff equivalents (by major trading partner) are estimated for the different pigmeat quarantine regimes implemented by Australia during the period 1988-2009. The welfare impact on consumers, producers, and foreign exporters is computed using a partial equilibrium model calibrated …
Evaluation Of Prebiotic Fibers And Pectin On Probiotic Strains And Effect Of Bacteriocin-Like Substance On The Survival Of Listeria Monocytogenes,
2012
University of Arkansas, Fayetteville
Evaluation Of Prebiotic Fibers And Pectin On Probiotic Strains And Effect Of Bacteriocin-Like Substance On The Survival Of Listeria Monocytogenes, Dolar Pak
Graduate Theses and Dissertations
Probiotics and prebiotics have been shown to provide beneficial effects on the host health and there are numerous research efforts being conducted on a variety of specific applications. However, most prebiotic carbohydrates are lower molecular weight oligosaccharides; and the health benefits of higher molecular weight pectins have not been fully evaluated. According to reviews, pectins are fermented in the large intestine due to the cooperative activities of colonic bacteria with complementary enzymatic activities. There are very few studies that demonstrate the fermentability of high methoxy pectin and low methoxy pectin by pure cultures of human isolates or probiotic bacteria. Thus, …
Lactic Acid Production Using Sweet Potato Processing Waste,
2012
University of Arkansas, Fayetteville
Lactic Acid Production Using Sweet Potato Processing Waste, Irene Pagana
Graduate Theses and Dissertations
Organic waste generated from industrial sweet potato canning is estimated to be 30% of incoming raw material. This waste contains carbohydrates (sugars and starch) that could be used as substrates for the production of useful compounds via fermentation (e.g. lactic acid), resulting in the production of value-added products. The goal of this research project is to produce a substrate from the sweet potato processing waste material that supports the growth of lactic acid bacteria, which results in the production of lactic acid. The sweet potato waste product was characterized, and found to contain 16.5% solids. The solids components were 18.5% …
The Effect Of Chlorine And Ozone In Controlling Microbes On Organically Grown Cilantro And Carrots,
2012
California Polytechnic State University, San Luis Obispo
The Effect Of Chlorine And Ozone In Controlling Microbes On Organically Grown Cilantro And Carrots, Adam Holtz
BioResource and Agricultural Engineering
This senior project investigates the relative efficacy of ozonated water and chlorinated water for the purpose of sanitizing freshly-harvested organic produce. The aim of this study was to assist the Cal Poly Organic Farm in deciding whether or not to invest in an ozone generator. The crops used for testing were grown organically in the same field. Carrots and cilantro were harvested and promptly treated with 5 ppm ozone solution or 25 ppm sodium hypochlorite solution. Carrots were sampled at 0, 7, and 14 days storage to determine microbial populations. Cilantro was similarly tested at 0, 6, and 12 days …
Effect Of Enzymatic Treatments On The Physiochemical Properties Of Different Corn Starches,
2012
University of Arkansas, Fayetteville
Effect Of Enzymatic Treatments On The Physiochemical Properties Of Different Corn Starches, Curtis Robert Luckett
Graduate Theses and Dissertations
Amylose readily reassociates to form films and crystalline structures that are resistant to digestion by amylolytic enzymes and known as resistant starch type III (RS3). This study investigated the RS3 formation and cereal coating properties from enzyme-modified corn starches with varying amylose contents, including Hylon VII (70% amylose), Hylon V (50% amylose), and common corn (25% amylose). For RS3 formation, corn starches were first gelatinized and then hydrolyzed using β-amylase to varying degrees. The resultant hydrolyzed starch was debranched with isoamylase and then exposed to 3 times of temperature cycling at 135/133/133°C for 30 min and 95°C for 24 hr …
Biological Strategies And Mathematical Approaches For Limiting Bacterial Contaminants And Chemical Pollutants In Bioethanol Fermentations,
2012
University of Arkansas, Fayetteville
Biological Strategies And Mathematical Approaches For Limiting Bacterial Contaminants And Chemical Pollutants In Bioethanol Fermentations, Alia Chahed Ep Limayem
Graduate Theses and Dissertations
During the most recent decades increased interest in fuel from biomass in the United States and worldwide has emerged each time petroleum derived-gasoline registered well publicized spikes in price. The willingness of the U.S. government to face the issues of more heavily high-priced foreign oil and climate change has led to more investment on plant-derived sustainable biofuel sources. Biomass derived from corn has become one of the primary feedstocks for bioethanol production for the past several years in the U.S. However, the argument of whether to use food as biofuel has led to a search for alternative non-food sources. Consequently, …
