Integrative Biology Commons^™

Induced Mutagenesis In West Nile Virus Non-Structural Protein 3 With A Discussion Of The Mutation's Effects On Viral Properties, Caden Connealy

Honors Program: Senior Projects (Public)

West Nile Virus (WNV), a member of the Flaviviridae family and introduced to the Western hemisphere in 1999, presents a unique and emerging threat to human health. Infection in humans is often mild but can occasionally result in severe neurological impairment and even death. West Nile Virus non-structural protein 3 (NS3) plays an important role in the process of viral genome amplification. More specifically, the C-terminal domain of the protein folds into a helicase enzyme, which is responsible for the “unwinding” of double-stranded RNA intermediates and RNA secondary structure on the WNV genome. This is a vitally important step in …

Trpv1 And Its Co‑Expressed Genes Are Associated With The Progression Of Hepatocellular Carcinoma, Bhuvnesh P. Sharma, Himanshu N. Singh, Sanjay Kumar, Deepak Verma, Deepak Parashar, Murali M. Yallapu, Everardo Cobos, Subhash C. Chauhan, Vivek K. Kashyap

Research Symposium

Background: Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Transient receptor potential vanilloid 1 (TRPV1) is a nonselective Ca²⁺ channel protein widely expressed and plays a significant role in cancer initiation and progression. However, the biological significance of TRPV1 in HCC has not been systematically and comprehensively investigated. This study used deep data mining and transcriptomics analyses to describe the significance of TRPV1 expression and its association with HCC prognosis.

Methods: Download the RNA expression of TRPV1 from the Cancer Genome Atlas (TCGA), Genotype Tissue Expression Atlas (GTEx), Tumor Immune Estimation Resource (TIMER), and the UALCAN …

Ms Environmental Biology Capstone: Evaluating Environmental Risks And Conflicts In Golf Course Landscapes, Meredith Boos

Regis University Student Publications (comprehensive collection)

1) This review investigates the relationship between pesticide use on golf courses and in agriculture to determine whether the adverse health effects documented in agricultural settings also apply to golf course environments. While the negative health effects from agricultural pesticide use are well established, golf courses use pesticides with greater frequency and intensity, suggesting a potentially higher exposure risk for both workers and players.

2) This study proposes methods to quantify pesticide bioaccumulation on golf courses and adjacent lands. We hypothesize that areas surrounding golf courses will exhibit a spatial gradient of pesticide accumulation, increasing with proximity to treated sites …

Genetic Influences On Individual Variability In Western Lowland Gorilla (Gorilla Gorilla Gorilla) Social Behavior, Hadley Mueller-Hill

Master's Theses

While primates are considered highly social animals, the evolutionary origins and mechanisms underlying their social behaviors remain unclear. Not only do they exhibit an extensive range of behaviors, but individuals within the same species can also vary widely in their social interactions. Genetics play a significant role in shaping these individual variations in behavior; multiple genetic variants have been associated with differences in social behavior in mammals. Examples of these include MAOA and OXTR genes, which influence the expression of behaviors associated with antisocial and prosocial temperaments. Genetic polymorphisms affect primate interactions, impacting an individual’s ability to maintain its status …

Diversification Of Avifauna In The Darién Highland Region: Ecological Niche Modeling, Phylogeography And Morphology To Explore Speciation In The Montane Species Myadestes Coloratus, Chlorospingus Tacarcunae And Margarornis Bellulus., Jorge L. Garzon Vasquez Mrs

Theses and Dissertations

Montane regions in the Neotropics are hotspots of endemism and biodiversity, yet their species are especially vulnerable to the impacts of climate change and geographic isolation. This thesis investigates the evolutionary and ecological dynamics of three bird species endemic to the Darién Highlands of eastern Panama: Myadestes coloratus, Chlorospingus tacarcunae, and Margarornis bellulus. Through an integrative approach combining ecological niche modeling, morphometric analysis, and genomic data from ultraconserved elements (UCEs) and mitochondrial DNA, the study explores the historical biogeography, current distribution patterns, population structure, and phenotypic differentiation of these montane taxa. Ecological niche models revealed that suitable habitat …

Assessing The Role Of Daily Thermal Fluctuations In The Performance Of Mitochondrial Energy Transduction Of The Centrarchid, Lepomis Cyanellus, Grayson Hofmann

Masters Theses

Freshwater systems are affected by current climate extremes, forcing species found in these systems to withstand drastic changes in temperature. Temperature changes affect these organisms by altering their metabolic rate and energy balance. Increases in temperature can lead to an increase in oxygen consumption, which ultimately increases the need for more energy production. Mitochondrial energy transduction of green sunfish (Lepomis cyanellus) and many other fish species after a thermal insult, has hardly been explored. This study complements the limited research available on mitochondrial function in similar species, as they ignore the possibility of consecutive thermal events, which are …

Florida Statewide Coastal Restoration Guide, Melinda J. Donnelly, Matthew Martin, Christen Fleming, Kelly Kibler, Linda Walters, Patrick Shearer, Lauren Jennison, Erika Lin, George Zaragoza

Faculty Scholarship and Creative Works

The Statewide Coastal Restoration Guide (SCRG) is a comprehensive planning resource developed through a collaboration between Florida Department of Environmental Protection (FDEP), University of Central Florida (UCF), and Resource Environmental Solutions (RES) to strengthen and unify shoreline restoration efforts across Florida. With over 4,500 miles of estuarine shoreline, Florida’s coasts are vital to the state’s ecological health, economic prosperity, and community resilience. However, they face increasing threats from sea level rise, stronger storms, erosion, habitat degradation, and development. The SCRG responds to these challenges by providing science-based, non-regulatory guidance for implementing resilient, nature-based restoration strategies that protect both ecosystems and …

Physiological Responses And Plant Trait Interactions With Coastal Dune And Swale Development, Emily C. Riffe

Theses and Dissertations

Vegetation is crucial to coastal ecosystems experiencing climate change and storm-induced disturbance. Plant species interact with the physical environment and are considered “ecosystem engineers.” Sand dunes and barrier islands are at the forefront of climate change impacts as they are barriers that protect landward habitat and communities. Nature based solutions are utilized to protect infrastructure on developed coastlines. Understanding how dominant plant species interact with and alter the physical environment enhances our understanding of the mechanisms of change and consequences to the system. The goal of this research was to quantify physiological and functional trait differences in dominant species found …

Florida Scrub Is Nuts (And Drupes): Fruit Production And Consumption In Florida’S Fire-Prone, Xeric Uplands, Warren G. Abrahamson Ii

Faculty Journal Articles

This is a story about how plant size, fire, and climate affect fruit and nut production (i.e., mast) in Florida’s fire-prone, xeric uplands and the importance of mast as food for wildlife. To set the stage, let’s first explore the primary mast-producing plants of Florida’s xeric uplands: oaks, hickory, and palmettos.

Elongate Locomotors Show Consistent Torque Patterns Across Substrates, Spencer Bradford, Henry Astley

Williams Honors College, Honors Research Projects

The four main movements found in snakes are understood mainly due to external study; however, muscular forces and resulting torques are poorly known. The main research objective is to study the torque production on different substrates using a snakebot. The study will give a rudimentary model for torque production on different substrates. It can then be compared against biological systems to understand snake movement at a muscular level. This study used a snakebot, allowing direct assessment of the current being run through each of its motors (proportional to torque). Data was then examined using a Principal Component Analysis test for …

Molecular Characterization Of Fungus-Arthropod Associations, Angie M. Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Arthropods, or phylum Arthropoda, are the largest group of animals, accounting for over three quarters of all animal records on NCBI Taxonomy. And yet, only 40% of all deposited sequences on NCBI Nucleotide come from arthropods. Clearly, arthropods are an understudied group of animals: they are small, often drab, and lead lives very alien to our own. Arthropods have chitinous exoskeletons, segmented bodies, and jointed appendages, and include crabs, barnacles, insects, spiders, and millipedes. Since the era of DNA based studies took off in the 1980s, DNA and other small molecules have been integral to our understanding of the Earth …

Assessing Genetic Diversity And Population Structure In The Imperiled Aconitum Reclinatum (White Monkshood), An Endemic Wildflower Of Central Appalachia, Diamanda A. Zizis

Master’s Theses

Aconitum reclinatum, white monkshood (Ranunculaceae), is a Pennsylvania critically imperiled plant species found in five states of the Appalachian region of the United States: Pennsylvania, West Virginia, Virginia, North Carolina, and Tennessee. This plant is adapted to cool, moist environments but faces threats from increasing habitat loss, deer browse, logging, and changes in rainfall and temperature associated with climate change. Comprehensive knowledge of A. reclinatum genetic population structure remains limited. To address this, we collaborated with Natural Heritage programs within the range of A. reclinatum to sample populations across the species distribution and generate a genotyping-by-sequencing data set of …

The Impacts Of Permafrost Thaw Slumps On Arctic Stream Ecology, Maria Dolan

Theses and Dissertations (Comprehensive)

Climate change is intensifying permafrost thaw across terrain of the circumpolar Arctic. Increased temperatures and rainfall at northern latitudes have resulted in the expansion of thermokarst features such as retrogressive thaw slumps, which make previously frozen debris available for transport into nearby river systems. In the Peel Plateau, Northwest Territories, the mobilization of these materials exposes rivers to a large quantity of sediment and solutes that may act as a chronic, press disturbance, potentially altering stream food webs. Here, I examine if: 1) thaw slumps have driven changes to the physical and biological properties of slump impacted streams since an …

Anoxia And Re-Oxygenation At Colder Temperatures In Goldfish (Carassius Auratus): Effects On Oxidative Stress And Metabolites, Matthew E. Trzcinski

Theses and Dissertations (Comprehensive)

Under the ice of ponds and lakes, goldfish (Carassius auratus) can survive weeks of anoxia at low temperatures by conserving ATP and by reducing their metabolic rate and relying on liver glycogen stores to meet their ATP demands using glycolysis. The following period of re-oxygenation is also known to be damaging in most vertebrates because it can lead to the generation of reactive oxygen species (ROS) that can cause oxidative damage to lipids, proteins and nucleic acids. Anoxia tolerant vertebrates such as the goldfish and crucian carp (Carassius carassius) accumulate reduced molecules with anoxia, overwhelming the …

Exploring The Salinity Tolerance Of Zooplankton Communities On The Great Plains, Abbeneet Binning Bath, Derek Gray

Theses and Dissertations (Comprehensive)

Palaeoecological data shows fluctuating salinity levels in lakes across the Great Plains due to alternating wet and dry periods over the last two millennia. Droughts caused by this natural variation may be exacerbated by anthropogenic climate change, as the Great Plains are expected to become drier over the coming decades. As conditions become drier, the evaporation of water will increase the salinity of lakes across the region. Increases in salinity may significantly impact biodiversity, including important members of lake food webs such as zooplankton. Due to the region's history of alternating wet and dry periods, zooplankton may have adapted to …

Lichen It: Optimizing Post-Fire Caribou Lichen Transplantation And Assessment, Elise Brown-Dussault

Theses and Dissertations (Comprehensive)

Caribou lichen species are common in mid- to late-seral boreal forests, serving as an important winter diet staple for boreal woodland caribou. However, as climate change causes boreal wildfires to increase in size, severity, and frequency, available tracts of mature forest are decreasing, diminishing the extent of mature caribou lichen stands. Caribou lichens reproduce vegetatively, so bigger and more severe fires could exacerbate their dispersal limitations, potentially extending the timeline for caribou lichen stand recovery beyond 80 years. Trials have shown that “transplanting” caribou lichen fragments into disturbed areas can lead to the establishment of caribou lichen mats; as such, …

Characterizing The Targeting Of Toc159 Receptors To The Chloroplast Outer Envelope Membrane And Their Role In The Assembly Of Distinct Toc Complexes, Michael Fish

Theses and Dissertations (Comprehensive)

Global agriculture and food security face challenges from climate change, population growth, and other human-driven pressures. Enhancing our understanding of plant biology, particularly chloroplast function, is critical for improving crop yield and stress resilience. As the sites of photosynthesis and key regulators of plant responses to environmental cues, chloroplasts depend on the precise import of thousands of nuclear-encoded proteins to maintain their biogenesis and function. While much is known about the core translocon components in the chloroplast outer envelope membrane that form the TOC complex, the mechanisms underlying their targeting, assembly, and regulation remain incompletely understood.

Plastids, including chloroplasts, evolved …

Macroinvertebrate Communities And Food Web Structure In Tundra Streams Are Shaped By Substrate Size And Beaver Impoundments, Mathew K. Mervyn

Theses and Dissertations (Comprehensive)

North American beavers (Castor Canadensis) are expanding their range into the Arctic tundra as climate change drives earlier ice and snowmelt, and increased shrub cover. As ecosystem engineers, beavers build dams that alter water chemistry by creating impoundments within streams, trapping sediment and organic matter, and modifying nutrient cycling. This thesis evaluates how beaver impoundments interact with natural geomorphic variation in tundra streams and how impoundments affect tundra freshwater ecosystems in the western Canadian Arctic.

In Chapter 2, I compared water chemistry and benthic macroinvertebrate (BMI) community composition across 16 stream reaches, including those with and without beaver dams, with …

Feeling Blue: The Effects Of Blue Light Exposure On Reproduction And Physiology In Drosophila Melanogaster, Laura Hampel

Theses and Dissertations (Comprehensive)

Blue light, the wavelengths ranging from ~400-500 nm, is emitted from the sun and more recently, by artificial lights including those in technology. In humans, prolonged exposure to blue light has been consistently linked to circadian rhythm disruption and retinal damage, and has also been associated with changes in neurodegeneration, aging, mortality, and oxidative stress in model organisms. This thesis explores the multifaceted effects of blue light exposure through two independent but complementary approaches. An initial rapid review of the current state of knowledge on blue light induced oxidative stress in humans was conducted. This revealed that the most studied …

The Prize Biter Of Modernity? Investigating Killer Whale Bite Mechanics, Daniel Hong

West Chester University Graduate Theses, Dissertations, and Final Projects

Although the killer whale (Orcinus orca) is a cosmopolitan apex predator, very little work has been done on its feeding biomechanics. Here I use finite element analysis to investigate the mandibular stiffness of a juvenile male Bigg’s killer whale, and compare it to that of the false killer whale (Pseudorca crassidens) and the saltwater crocodile (Crocodylus porosus). Full mandibles and left and right halves of mandibles are simulated under FEA. I also estimate the total jaw muscle force produced by the Bigg’s killer whale using the dry skull method. The killer whale lacks an …