Agriculture Commons^™

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such …

Simulating Long-Term Impacts Of Cover Crops And Climate Change On Crop Production And Environmental Outcomes In The Midwestern United States, Andrea D. Basche, Sotririos V. Archontoulis, Thomas C. Kaspar, Dan B. Jaynes, Timothy B. Parkin, Fernando E. Miguez

Department of Agronomy and Horticulture: Faculty Publications

It is critical to evaluate conservation practices that protect soil and water resources from climate change in the Midwestern United States, a region that produces one-quarter of the world’s soybeans and one-third of the world’s maize. An over-winter cover crop in a maize–soybean rotation offers multiple potential benefits that can reduce the impacts of higher temperatures and more variable rainfall; some of the anticipated changes for the Midwest. In this experiment we used the Agricultural Production Systems sIMulator (APSIM) to understand how winter rye cover crops impact crop production and environmental outcomes, given future climate change. We first tested APSIM …

Decipher The Molecular Response Of Plant Single Cell Types To Environmental Stresses, Mehrnoush Nourbakhsh-Rey, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

The analysis of the molecular response of entire plants or organs to environmental stresses suffers from the cellular complexity of the samples used. Specifically, this cellular complexity masks cell-specific responses to environmental stresses and logically leads to the dilution of the molecular changes occurring in each cell type composing the tissue/organ/plant in response to the stress.Therefore, to generate a more accurate picture of these responses, scientists are focusing on plant single cell type approaches. Several cell types are now considered as models such as the pollen, the trichomes, the cotton fiber, various root cell types including the root hair cell, …

Draft Genome Sequence Of Erwinia Tracheiphila, An Economically Important Bacterial Pathogen Of Cucurbits, Lori R. Shapiro, Erin D. Scully, Dana Roberts, Timothy J. Straub, Scott M. Geib, Jihye Park, Andrew G. Stephenson, Erika Salaau Rojas, Quin Liu, Gwyn Beattie, Mark Gleason, Consuelo M. De Moraes, Mark C. Mescher, Shelby G. Fleischer, Roberto Kolter, Naomi Pierce, Olga Zhaxybayeva

Department of Agronomy and Horticulture: Faculty Publications

Erwinia tracheiphila is one of the most economically important pathogens of cucumbers, melons, squashes, pumpkins, and gourds in the northeastern and midwestern United States, yet its molecular pathology remains uninvestigated. Here, we report the first draft genome sequence of an E. tracheiphila strain isolated from an infected wild gourd (Cucurbita pepo subsp. texana) plant. The genome assembly consists of 7 contigs and includes a putative plasmid and at least 20 phage and prophage elements.

Nebraska Farm Real Estate Market Highlights 2015-2016, Jim Jansen, Roger Wilson

Nebraska Farm Real Estate Reports

The Nebraska Farm Real Estate Market Highlights 2015-2016 report represents the 38th edition to the annual series. These reports provide an important insight on agricultural land market dynamics for stakeholders across Nebraska. In today's market, where market transactions exceeding a million dollars are the norm, objective market information and analysis is more critical than ever. The focus of the report continues to provide unbiased information on agricultural land values and rental rates so industry participants can make educated and informed decisions.

This year, the February 2016 survey of over 100 expert-panel members from across the state provided current information and …

Muts Homolog1 Silencing Mediates Orf220 Substoichiometric Shifting And Causes Male Sterility In Brassica Juncea, Na Zhao, Xinyue Xu, Yashitola Wamboldt, Sally Ann Mackenzie, Xiaodong Yang, Zhongyuan Hu, Jinghua Yang, Mingfang Zhang

Department of Agronomy and Horticulture: Faculty Publications

Cytoplasmic male sterility (CMS) has consistently been associated with the expression of mitochondrial open reading frames (ORFs) that arise from genomic rearrangements. Spontaneous fertility reversion in CMS has been observed in several cases, but a clear understanding of fertility reversion controlled by nuclear genetic influences has been lacking. Here, we identified spontaneous fertile revertant lines for Brassica juncea CMS cytoplasm in which the mitochondrial genome has undergone substoichiometric shifting (SSS) to suppress ORF220 copy number. We placed ORF220, with or without a mitochondrial targeting presequence, under the control of the CaMV35S and AP3 promoters in Arabidopsis to confirm that ORF220 …

Western Australian Biosecurity Strategy 2016 - 2025, Department Of Agriculture And Food, Western Australia

Biosecurity published reports

Vision

The vision of WA’s Biosecurity Strategy is ‘working together to minimise the risks to the state’s economic development, environmental assets and social amenity from terrestrial and aquatic pests and diseases’.

Purpose

The purpose of the Strategy is to set the overall direction for the management of emerging and ongoing biosecurity issues within WA from 2016 to 2025.

Scope

The Strategy covers animal and plant pests, diseases and weeds, and zoonotic diseases that can be transmitted between animals and humans. These have the potential to negatively affect WA’s economy, terrestrial and aquatic environments, biodiversity, agricultural resources, human health and social …

Investigating Ichneumonidae: Insights Into Species Identification And Venom Composition, Victoria G. Pook

Theses and Dissertations--Entomology

Parasitoid wasps are hyperdiverse, with current estimates suggesting that they may account for up to 20% of all insect species. Though their ecological significance and their importance in integrated pest management cannot be denied, these taxa remain understudied and, due to their small size, are often overlooked. However, recent advances in molecular techniques are helping to reverse this trend by providing tools which scientists can use to better understand species limits and host interactions.

Parasitoid wasps are often morphologically cryptic and their accurate delimitation requires the analysis of DNA sequence data from fast-evolving genes in addition to morphological characters. The …

Soil Health Within Indianapolis Urban Gardens, Blake Moskal

Undergraduate Honors Thesis Collection

The growing trend of healthier diets and localized food systems has led to the emergence of many urban farms throughout Indianapolis. Communities now have access to arable land where they can grow high quality produce for families and communities. However, many of the farms are built on land with past industrial or commercial legacies. These postindustrial soils could contain contaminants, like heavy metals, some of which are potentially harmful to humans. These sites also have poor soil quality, so farmers often have to import soil and use large amounts of fertilizer or compost to ensure viable growing conditions. To isolate …

Modeling The Compressibility Behavior Of Hard Red Wheat Varieties, Aaron P. Turner, Michael D. Montross, Samuel G. Mcneill, Michael P. Sama, Mark E. Casada, Josephine M. Boac, Rumela Bhadra, Ronaldo G. Maghirang, Sidney A. Thompson

Biosystems and Agricultural Engineering Faculty Publications

The bulk density of grain in a storage structure varies vertically and horizontally due to the overburden pressure created by the cumulative weight of the overlying material. As the overburden pressure increases, the stored material compacts. This compaction is believed to be caused by rearrangement of kernels along with higher intergranular stress between particles, leading to kernel deformation. This compaction is of primary concern when estimating the amount of grain in a storage structure. In this comprehensive study, confined uniaxial compression tests were conducted on 27 different samples of hard red winter wheat, at three moisture levels, over the range …

Error Analysis Of Stored Grain Inventory Determination, Aaron P. Turner, Michael D. Montross, Joshua J. Jackson, Samuel G. Mcneill, Mark E. Casada, Josephine M. Boac, Rumela Bhadra, Ronaldo G. Maghirang, Sidney A. Thompson

Biosystems and Agricultural Engineering Faculty Publications

Estimation of the quantity of stored grain is important for crop insurance, financial statements, and inventory control. Traditionally, the height of grain has been measured using weighted tape measures, and the volume is subsequently computed using standard geometric shapes (cylinders and cones) along with visual correction of the grain surface. Field measurements by four trained USDA Farm Service Agency and crop insurance agents on older farm-sized bins (8.2 to 11.0 m, or 27 to 36 ft, in diameter) resulted in standard deviations between 0.02 and 0.30 m for the equivalent height when the grain surface was not level. The largest …

Correlating Bulk Density (With Dockage) And Test Weight (Without Dockage) For Wheat Samples, Rumela Bhadra, Mark E. Casada, Josephine M. Boac, Aaron P. Turner, Sidney A. Thompson, Michael D. Montross, Ronaldo G. Maghirang, Samuel G. Mcneill

Biosystems and Agricultural Engineering Faculty Publications

In grain bins, the compaction of stored grain is caused by the overbearing pressure of the bulk material in the bin. To predict the amount of grain in the bin, compaction values must be determined based on the average bulk density (BD) of the stored material. However, BD is determined following the Federal Grain Inspection Service (FGIS) guidelines for measuring test weight (TW), which require that dockage be removed prior to measuring wheat TW. Thus, this creates a problem for predicting grain compaction and conducting inventory studies, because the average BD of the grain in a bin for these calculations …

As-Applied Estimation Of Volumetric Flow Rate From A Single Sprayer Nozzle Series Using Water-Sensitive Spray Cards, Michael P. Sama, John T. Evans, Aaron P. Turner, Surya Saket Dasika

Biosystems and Agricultural Engineering Faculty Publications

The objective of this study was to test the feasibility of using coverage measurements from water-sensitive spray cards to estimate the volumetric flow rate at an individual sprayer nozzle. TeeJet VisiFlow Even Flat Spray Tips were selected due to their uniform distribution of coverage. Spray distribution for each nozzle was validated using a spray patternator table with 2.5 cm sampling widths. A rotary test fixture translated water-sensitive spray cards through the spray dispersion (water at ambient conditions) at a constant angular velocity and a radius of 1.2 m. The test fixture measured volumetric flow and pressure at the nozzle and …

A Review Of Non-Destructive Methods For Detection Of Insect Infestation In Fruits And Vegetables, Nader Ekramirad, Akinbode A. Adedeji, Reza Alimardani

Biosystems and Agricultural Engineering Faculty Publications

Insect damage in fruits and vegetables cause major production and economic losses in the agriculture and food industry worldwide. Monitoring of internal quality and detection of insect infestation in fruits and vegetables is critical for sustainable agriculture. Early detection of an infestation in fruits can facilitate the control of insects and the quarantine operations through proper post-harvest management strategies and can improve productivity. The present review recognizes the need for developing a rapid, cost-effective, and reliable insect infestation monitoring system that would lead to advancements in agriculture and food industry. In this paper, an overview of non-destructive detection insect damages …

Home Vegetable Gardening In Kentucky [2016], Richard Durham, Shubin Saha, John Strang, Mark A. Williams, Shawn Wright, Ricardo Bessin, Brad Lee, Emily Pfeufer

Agriculture and Natural Resources Publications

Every aspiring gardener should follow seven steps to have a successful gardening season:

1. Plan your garden on paper before you begin.
2. Select a good gardening site that is:
   a. in full sun for at least eight hours each day,
   b. relatively level,
   c. well‑drained,
   d. close to a water source,
   e. dries quickly from morning dew.
3. Prepare the soil properly, conduct a soil test, and add fertilizer and lime according to U.K. test result recommendations.
4. Plan only as large a garden as you can easily maintain. Beginning gardeners often overplant, and then they fail because they cannot keep up with …

2016 – 2017 Kentucky Agricultural Economic Situation And Outlook, Kenneth H. Burdine, Todd D. Davis, Timothy A. Woods, William M. Snell, Lee Meyer, Jeffrey W. Stringer, Bobby Ammerman

Agricultural Situation and Outlook

Topics:

• U.S. Agricultural Economy;
• Kentucky’s Agricultural Economy.

An Investigation Of The Use Of Natural Agents To Control Mycobacteria, Olga Lesniak

Theses

Economic losses that are caused by Mycobacterium avium paratuberculosis (MAP) infections represent a major obstacle for farmers and also for the dairy industry. In order to control the population of MAP, alternative control agents must be investigated. One of the studies described in this thesis focused on using a bacteriophage therapy based approach to assess its efficacy on simulated industrial environments and food models. Another study described in this thesis investigated the antimicrobial effects of parsley and garlic on a range of mycobacteria species. Both studies used conventional microbiological methods such as plaque assays, AlamarBlue assay (MABA) and growth curves, …

Temporal And Spatial Influences On South Dakota Farm Economies Of Size, Henry Brown

Electronic Theses and Dissertations

Census of Agriculture data are evaluated to test for economies of size of South Dakota farms. Economies of size occur when per-unit costs decrease as the size of a firm increases. This research measures technical economies of size, which assume that all firms pay identical input prices and receive identical output prices. The dependent variable of the model is expenses per dollar of sales. This is a typical measure of efficiency in agricultural statistics. The data come from the four prior Census of Agriculture years, 1997, 2002, 2007, and 2012. A state-wide model and a county-wide model were developed to …

Long-Term Impacts Of Annual Cattle Manure And Fertilizer On Soil Quality Under Corn-Soybean Rotation In Eastern South Dakota, Ekrem Ozlu

Electronic Theses and Dissertations

Dairy and beef manure have been used to enhance soil quality; however, their impacts under long-term application in corn-soybean rotation need to be evaluated. Nutrient based recommended rates of manure applications on soils are important and also need to be monitored. This study, therefore, was conducted at two long-term sites to assess the impacts of manure and inorganic fertilizer application rates on some of the soil quality indicators and greenhouse gas emissions (GHGs) in a corn (Zea mays L.) - soybean (Glycine max L.) rotation system located at Beresford and Brookings in Eastern South Dakota. Study treatments included: …

Demonstrating Short-Term Impacts Of Grazing Cover Crops On Soil Health In South Dakota, Colin Tobin

Electronic Theses and Dissertations

Grasslands have been rapidly converted to croplands over the last decade in the northern Great Plains. This conversion can reduce soil health and increase the region’s ability to pollute the Missouri and Mississippi rivers. Therefore, the need for integrated crop livestock (ICL) practices that can protect the region’s native prairies are strongly encouraged. Introducing livestock into arable cropping systems can improve nutrient cycling, soil health, and provide economic benefits. However, the detailed information about the impacts of ICL system on soil health is still lacking in the Northern Great Plains region. Therefore, the present study was conducted under a corn …