Agriculture Commons^™

Phenotypes Of Macrophages Present In The Intestine Are Impacted By Stage Of Disease In Cattle Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Caitlin J. Jenvey, Adrienne L. Shircliff, J. P. Bannantine, Judith R. Stabel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Macrophages play an important role in the host immune response to Mycobacterium avium subsp. paratuberculosis (MAP) infection, however, MAP is able to disrupt normal macro- phage functions to avoid destruction. It is unclear whether the phenotypes of macrophages present in the target tissue play a role in the inability to clear MAP infection. The aim of this study was to identify macrophage phenotypes (host defense or resolution and repair) present within the bovine ileum of naturally infected cattle, as well as to ascertain abundance of each macrophage phenotype present during different stages of MAP infection. Immunofluo- rescent (IF) labeling was …

Predicting Spatial Risk Of Wolf-Cattle Encounters On Rugged And Extensive Grazing Lands, Patrick R. Clark, Joe Chigbrow, Douglas E. Johnson, John Williams, Larry L. Larson, Tyanne Roland

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cattle grazing lands in the mountainous western United States are rugged, complex, and extensive. Terrain, vegetation, and other landscape features vary greatly across space. Risk of wolf-cattle encounters and potential for depredation loss certainly differ spatially as consequence of this variability. Yet, our understanding of this spatial risk is quite poor and this knowledge gap severely hampers our abilities to manage wolf-livestock interactions and mitigate conflicts. During 2009-2011, a research study was conducted at four study areas (USFS cattle grazing allotments) in western Idaho to evaluate and predict risk of wolf-cattle encounters. Each year, a random sample of 10 lactating …

Characterization Of Ethanol Extracted Cell Wall Components Of Mycobacterium Avium Subsp. Paratuberculosis, John P. Bannantinea, Ashutosh Wadhwa, Judith R. Stabel, Shigetoshi Eda

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Antigens extracted using ethanol (EtOH) and incorporated in the EtOH vortex ELISA (EVELISA) test have previously shown high specificity and sensitivity for detecting Mycobacterium avium subspecies paratuberculosis (Map) and M. bovis infections in cattle. The objective of this study is to define the components present in the EtOH extract. We show that this extract is composed of lipid, carbohydrate, and proteins on the surface of the bacilli, and that EtOH removes the outer layer structure of Map which comprise these elements. To identify proteins, polyclonal antibodies to the EtOH prep were produced and used to screen a Map genomic expression …

Casaicinoid Yield And The Effect Of Growth Hormones In Peppers, Jordan Szabat

Summer Community of Scholars Posters (RCEU and HCR Combined Programs)

No abstract provided.

Comparison Of Maize Lines And Their Test Crosses According To Grain Yield And Somephysiological Properties, Eli̇f Özdemi̇r, Bayram Sade

Turkish Journal of Agriculture and Forestry

This study evaluated 7 inbred lines of maize (Zea mays L.), 3 testers, and 21 hybrids produced using the line × tester mating design. The study was conducted in Konya, in The Central Anatolia Region of Turkey. CD (coefficient of determination) values were calculated to detect the effect of inbred lines on grain yield and other physiological properties of the test crosses. The mean values of all the parameters of the genotypes were evaluated using the Duncan?s multiple range test for grouping progenies and parent groups. The CD values between parent groups and progenies were statistically significant for photosynthetic efficiency …

Design, Development, And Evaluation Of A Target Oriented Weed Control System Usingmachine Vision, Ömer Bariş Özlüoymak, Ali̇ Bolat, Ali̇ Bayat, Emi̇n Güzel

Turkish Journal of Agriculture and Forestry

The objective of the study was to develop and test an automatic machine vision-based spraying robot for the detection, tracking, and spraying of artificial weeds by using LabVIEW programming language. The greenness method was used to distinguish green objects in the image. A time-controlled spray nozzle was run according to the presence of an artificial weed and its coordinates. A mobile test bench was built and the spraying system with a webcam was operated at speeds of 0.42, 0.54, 0.66, 0.78, and 0.90 km h-1, so as to be able to see the performance of the system. The amount of …

Highly Polymorphic Novel Simple Sequence Repeat Markers From Class I Repeats Inwalnut (Juglans Regia L.), Emre Eser, Hayat Topçu, Sina Kefayati, Mehmet Sütyemez, Md Rashedul Islam, Sali̇h Kafkas

Turkish Journal of Agriculture and Forestry

Walnut (Juglans regia L.) is the most important species in the genus Juglans due to the high commercial value of its nuts and timber. Simple sequence repeats (SSRs) are considered as the markers of choice owing to their codominant nature. Since very few SSRs have been developed in walnut, this study aimed to develop numerous polymorphic SSRs from Class I repeats by using DNA sequences of the Chandler cultivar. In all, 800 SSRs were designed and tested in 8 Turkish, 3 French, and 5 US walnut cultivars, of which 88 (11%) did not produce bands, 161 (20.1%) were monomorphic, and …

Improvement Of Rebaudioside A Diterpene Glycoside Content In Stevia Rebaudianabertoni Using Clone Selection, Begüm Kaplan, Kenan Turgut

Turkish Journal of Agriculture and Forestry

This study, conducted in Antalya, Turkey, between 2014 and 2016, aimed to determine genotypes with a high amount of rebaudioside A (reb A) and high reb A/stevioside (stv) ratio in Stevia rebaudiana Bertoni, and reveal superior candidate varieties via the clone selection method. The study started with 200 genotypes in the first year, followed by the stem cuttings of 40 genotypes selected in the second year, in accordance with the obtained data, which were grown in rows (A clones), and the 10 genotypes (B clones) selected from the clones in the third year, which were grown in 3 replications according …

Effects Of Moisture Content, Internode Region, And Oblique Angle On The Mechanicalproperties Of Sainfoin Stem, Mustafa Gökalp Boydaş, Mustafa Çomakli, Bahadir Sayinci, Mazhar Kara

Turkish Journal of Agriculture and Forestry

The effects of moisture content, internode region, and oblique angle on several mechanical properties of sainfoin stem were determined. The selected mechanical properties used were the shearing stress, specific shearing energy, bending stress, and modulus of elasticity when bending. The experiments were carried out with wet basis (WB) moisture contents of 71.76%, 45.57%, and 25.57%, at the third and second internode regions from the bottom up to the top, and 2 oblique angles (0° and 28°). The research results showed that the shearing stress of sainfoin stem increased as the moisture content increased, but decreased towards the upper internode of …

Role Of Endogenous Polyamines In The Alternate Bearing Phenomenon In Pistachio, Muhammet Ali̇ Gündeşli̇, Sali̇h Kafkas, Mouzghan Zarifikhosroshahi, Nesi̇be Ebru Kafkas

Turkish Journal of Agriculture and Forestry

Alternate bearing is a significant economic problem adversely affecting the producers, consumers, and economy of a country. In the present study, we aimed to investigate the physiological role of free polyamines (PAs) in fruit bud abscission of the Uzun pistachio variety, which has a strong alternate bearing tendency. The identification and quantification of PAs, such as putrescine (Put), spermidine (Spd), and spermine (Spm), at different physiological stages and in different organs, were carried out by high-performance liquid chromatography. Putrescine was the major PA in all the organs of pistachio trees. Furthermore, the concentrations of Put and Spd in the leaves …

Effects Of Rootstocks On Storage Performance Of Nova Mandarins, Ahmet Erhan Özdemi̇r, Öznur Di̇di̇n, Eli̇f Çandir, Mustafa Kaplankiran, Ercan Yildiz

Turkish Journal of Agriculture and Forestry

In this research, the effects of rootstocks on the storage performance of Nova mandarins grafted on Carrizo citrange, Troyer citrange, or sour orange grown in the ecological conditions of Dörtyol were studied. Fruits were harvested at optimum maturity and kept at 4 °C and 6 °C for 120 days. Changes in weight loss, incidence of fungal decay and physiological disorders, juice content, total soluble solids (TSS), percent fruit with green button, titratable acidity (TA), juice pH, vitamin C content, and rind color (L*, C*, h°) were monitored at 15-day intervals during storage to determine the effects of rootstock on postharvest …

The Development Of A Low Cost Uav-Based Image Acquisition System And The Procedure For Capturing Data In Precision Agriculture, Ari̇f Behi̇ç Teki̇n, Marco Fornale

Turkish Journal of Agriculture and Forestry

Remote sensing is a method of monitoring the natural heterogeneity of vegetation. Although satellite based remote sensing has been a popular method for monitoring the earth's surface, it has several drawbacks, such as the orbital period, unattended capture, and investment cost. On the other hand, an unmanned air vehicle (UAV) is more flexible in terms of deployment, monitoring a small area, and being easy to obtain at a low cost. From this point of view, the goal of this research was to develop a low cost and easy to implement technical solution for mapping spatial heterogeneity and research its relationship …

Effects Of Methyl Jasmonate And Salicylic Acid On The Production Of Camphor And Phenolic Compounds In Cell Suspension Culture Of Endemic Turkish Yarrow(Achillea Gypsicola) Species, Muhammed Aki̇f Açikgöz, Şevket Meti̇n Kara, Ahmet Aygün, Mehmet Muharrem Özcan, Ebru Bati Ay

Turkish Journal of Agriculture and Forestry

Elicitors have been widely used as biotic and abiotic stimulants in triggering the production of secondary metabolites in plant cell culture. The present study aimed to enhance the production of camphor and phenolic compounds and cell growth using methyl jasmonate (MeJA) and salicylic acid (SA) in the cell suspension culture of Turkish endemic species Achillea gypsicola. Various concentrations (0, 10, 50, and 100 μM) of MeJA and SA were applied to 8-day-old cell cultures. The camphor and phenolic compound contents were determined using a headspace gas chromatographic-mass spectrometer device and spectrophotometer. Increasing doses of MeJA and SA significantly enhanced the …

Effects Of Tillage Method And Fertilizer Type On The Yield Of Sudan Grass(Sorghum Bicolor L.), Bojan Stipesevic, Bojana Brozovic, Danijel Jug, Irena Jug, Vesna Vukadinovic, Boris Djurdjevic

Turkish Journal of Agriculture and Forestry

The Sudan grass plant (Sorghum bicolor (L.) Moench subsp. drummondii) density and yield were determined in this 2-year research with different tillage systems and fertilization at 3 locations. After harvesting the main crops, 3 tillage treatments were applied: conventional (CT) mouldboard ploughing (25-30 cm deep), followed by disk harrowing and sowing: reduced tillage 1 (HDH) with 2 passes by a disk harrow (15-20 cm deep), followed by seedbed preparation, and reduced tillage 2 (LDH) with a single pass by a disk harrow. Soil penetration resistance was measured at each tillage treatment. A total of 5 side dressing fertilizer treatments were …

Determination Of Essential Oil Components, Mineral Matter, And Heavy Metal Content Ofsalvia Virgata Jacq. Grown In Culture Conditions, Belgi̇n Coşge Şenkal, Tansu Uskutoğlu, Cüneyt Cesur, Volkan Özavci, Hülya Doğan

Turkish Journal of Agriculture and Forestry

Most members of the family Lamiaceae are of considerable importance in areas such as medicine, food, cosmetics, and perfumery, as they are rich in essential oils, aromatic oils, and secondary metabolites. Sage, a member of this family, is the generic name for species of the genus Salvia. The genus Salvia has approximately 900 species worldwide. There are 99 species of the genus Salvia L. in Turkey; 51 of these species are endemic. The local name of S. virgata is "yılancık" or "fatmanaotu" in Turkey, and it is used for the treatment of wounds and various skin diseases. In addition, a …

Effect Of Cultivar And Fertilization On Garlic Yield And Allicin Content In Bulbs At Harvestand During Storage, Nina Kacjan Marsic, Marijan Necemer, Robert Veberic, Natasa Poklar Ulrih, Mihaela Skrt

Turkish Journal of Agriculture and Forestry

Allicin, one of the most important flavor compounds in garlic bulbs, is affected by genotypic characteristics modified by ontogenesis, environmental conditions, fertilization practices, and storage duration. Therefore, the effects of fertilization practices and cultivar on garlic yield and allicin content in garlic bulbs were evaluated at harvest and during storage. One autochthonous (Ptujski spomladanski) and three commercial cultivars (Garcua, Gardacho, and Gardos) were used, and three fertilization treatments (N90S0 - 90 kg N ha-1, N90S60 - 90 kg N ha-1 +60 kg S ha-1, and N0S0 - control) were performed during two growing seasons. After the harvest, 32 bulbs per …

Utilization Of Sucrose During Cocultivation Positively Affects Agrobacterium-Mediatedtransformation Efficiency In Sugar Beet (Beta Vulgaris L.), Songül Gürel, Muhammet Çağri Oğuz, Ferzat Turan, Kemal Kazan, Canan Yüksel Özmen, Ekrem Gürel, Ali̇ Ergül

Turkish Journal of Agriculture and Forestry

Sugar beet (Beta vulgaris L.) is one of the most important industrial crops throughout world. With the availability of suitable genetic transformation technologies, the yield, quality, and stress tolerance of sugar beet could be improved significantly. However, low transformation efficiencies seriously limit the application of molecular technologies to the genetic improvement of sugar beet. With the aim of improving gene transfer techniques for sugar beet, the effect of different sucrose concentrations during cocultivation on the initial Agrobacterium-mediated transformation efficiencies in sugar beet was tested. To develop an efficient experimental system through which the effect of sucrose could be tested, first, …

New Technology (In Situ Grafting) For Faster Production Of Walnut (Juglans Regia L.), Borivoje Bogdanovic, Sandra Bijelic, Branislava Golosin, Dijana Kulacanin

Turkish Journal of Agriculture and Forestry

Technology regarding grafted walnut production is very complex and expensive because it depends on a number of factors that directly influence grafting success. Due to a long production period and a smaller number of first class plants compared to other fruit species, young walnut trees are among the most expensive. New in situ production technology of young walnut trees has led to quicker production, shorter by 1 year, and increased the success of grafting, allowing for large-scale production of grafted walnut. In order to increase the production of quality planting material for walnut varieties, the possibility of walnut grafting in …

Combined Effects Of Ethephon And Mepiquat Chloride On Late Blooming, Fruit Set, Andphytochemical Characteristics Of Black Diamond Plum, Kerem Mertoğlu, Yasemi̇n Evrenosoğlu, Mehmet Polat

Turkish Journal of Agriculture and Forestry

This study was carried out in 2015 and 2016 to investigate the use of ethephon and mepiquat chloride for reduction of damage to flowering and fruit set caused by late spring frosts in Black Diamond plum. The single treatment of mepiquat chloride delayed full blooming by 5 days, while the ethephon treatment delayed blooming by 7 days. The interactive effect of the chemicals yielded more positive results in the delay of blooming, causing a delay of up to 11 days. An increase in fruit set was observed along with blooming. The fruit set percentage of the control was 4.18%, while …

Nebline, January 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

2018 Extension highlights

Benefit to people

Teaching, growing and making a difference

36,520 youth engaged in 4-H programs

Extension’s volunteers worth nearly $700,000

New Year, new you: Nutrition tips to a healthier you

RECIPE OF THE MONTH

Pesticide applicator 2019 training dates

Ag Estate and Succession Planning workshop, Jan. 23 & March 5

Successful Farmer Series

Upcoming green industry conferences

Extension’s 2019 Master Gardener trainings, two sites available

Garden Guide Things to Do This Month

Sticky situation: Using glue boards to monitor pests

HEART OF 4-H Volunteer Award: Susan Bulling

IT’S TIME TO RE-ENROLL IN 4-H!

Meet 2018–2019 4-H Council …