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P2x7 Receptor Activation Mediates Organic Cation Uptake Into Human Myeloid Leukaemic Kg-1 Cells, Safina Gadeock, Aleta Pupovac, Vanessa Sluyter, Mari Spildrejorde, Ronald Sluyter Jan 2012

P2x7 Receptor Activation Mediates Organic Cation Uptake Into Human Myeloid Leukaemic Kg-1 Cells, Safina Gadeock, Aleta Pupovac, Vanessa Sluyter, Mari Spildrejorde, Ronald Sluyter

Faculty of Science - Papers (Archive)

The P2X7 purinergic receptor is an ATP-gated cation channel with an emerging role in neoplasia. In this study we demonstrate that the human KG-1 cell line, a model of acute myelogenous leukaemia, expresses functional P2X7. RT-PCR and immunochemical techniques demonstrated the presence of P2X7 mRNA and protein respectively in KG-l cells, as well as in positive control multiple myeloma RPMI 8226 cells. Flow cytometric measurements demonstrated that ATP induced ethidium(+) uptake into KG-l cells suspended in sucrose medium (EC(50) of ∼3 μM), but not into cells in NaCl medium. In contrast, ATP induced ethidium(+) uptake into RPMI 8226 cells suspended …


Terrestrial Hermit Crabs (Anomura: Coenobitidae) As Taphonomic Agents In Circum-Tropical Coastal Sites, Katherine Szabo Jan 2012

Terrestrial Hermit Crabs (Anomura: Coenobitidae) As Taphonomic Agents In Circum-Tropical Coastal Sites, Katherine Szabo

Faculty of Science - Papers (Archive)

Hermit crabs are ever alert for more suitable shells to inhabit, but what this may mean for coastal shell middens has rarely been considered. Here, the impact of the most landward-based of hermit crab families, the tropical Coenobitidae, upon archaeological shell-bearing deposits is assessed using a case study: the Neolithic Ugaga site from Fiji. At Ugaga, hermit crabs were found to have removed the majority of shells from the midden and had deposited their old, worn shells in return. The behavioural ecology of genus Coenobita suggests a mutualistic interaction whereby humans make available shell and food resources to hermit crabs, …


Slip Rate Of The Aksay Segment Of Altyn Tagh Fault Revealed By Osl Dating Of River Terraces, Yiwei Chen, Sheng-Hua Li, Bo Li Jan 2012

Slip Rate Of The Aksay Segment Of Altyn Tagh Fault Revealed By Osl Dating Of River Terraces, Yiwei Chen, Sheng-Hua Li, Bo Li

Faculty of Science - Papers (Archive)

The slip rate of Altyn Tagh Fault (ATF) was studied near the Aksay segment (39°24.572´N, 94°16.012´E), China, based on dating the terraces of a river passing through the ATF. Two river terrace risers were offset by the ATF and the fault displacements were recorded. Average slip rate of the Aksay segment of the ATF was estimated using the offset of terrace risers divided by the corresponding ages. The ages of the terraces were determined by optical dating of the loess deposited on the river terrace. Our results demonstrated that: (1) The optically stimulated luminescence (OSL) ages of loess can be …


Testing A Multi-Step Post-Ir Irsl Dating Method Using Polymineral Fine Grains From Chinese Loess, Xiao Fu, Bo Li, Sheng-Hua Li Jan 2012

Testing A Multi-Step Post-Ir Irsl Dating Method Using Polymineral Fine Grains From Chinese Loess, Xiao Fu, Bo Li, Sheng-Hua Li

Faculty of Science - Papers (Archive)

The potential of multi-elevated-temperature post-IR IRSL (MET-pIRIR) dating [Li, B., Li, S.H., 2011. Luminescence dating of K-feldspar from sediments: a protocol without anomalous fading correction. Quaternary Geochronology 6, 468-479] using polymineral fine grains (FG) (4-11 μm) is tested using loess samples from the Luochuan section in the Chinese Loess Plateau. Nine FG samples with ages within the last glacial-interglacial period are tested using the MET-pIRIR protocol. The MET-pIRIR results for FG are compared with both the coarse grain (63-90 μm) K-feldspar MET-pIRIR dating results and the coarse grain quartz OSL dating results. The stratigraphic age of the profile also provides …


The Synthesis, Structures And Reactions Of Zinc And Cobalt Metal-Organic Frameworks Incorporating An Alkyne-Based Dicarboxylate Linker, Andrew D. Burrows, L C. Fisher, David M. Hodgson, Mary F. Mahon, Naomi Cessford, Tina Duren, Christopher Richardson, S P. Rigby Jan 2012

The Synthesis, Structures And Reactions Of Zinc And Cobalt Metal-Organic Frameworks Incorporating An Alkyne-Based Dicarboxylate Linker, Andrew D. Burrows, L C. Fisher, David M. Hodgson, Mary F. Mahon, Naomi Cessford, Tina Duren, Christopher Richardson, S P. Rigby

Faculty of Science - Papers (Archive)

The reaction of zinc(II) nitrate and 4,4′-ethynylenedibenzoic acid (H2edb) in DMF at 80 °C gave the metal–organic framework material [Zn4O(edb)3(H2O)2]·6DMF 1 in which edb ligands connect Zn4O centres into a doubly-interpenetrated cubic network with a similar topology to observed with other linear dicarboxylates in the IRMOF series. Analysis of the nitrogen isotherm revealed the material to have a BET surface area of 1088 m2 g−1, which is approximately one-third of the value calculated from GCMC simulations, suggesting incomplete activation or pore blocking in the activated …


Using Remotely-Sensed Fuel Connectivity Patterns As A Tool For Fire Danger Monitoring, Gabriele Caccamo, Laurie A. Chisholm, Ross A. Bradstock, Marjetta L. Puotinen Jan 2012

Using Remotely-Sensed Fuel Connectivity Patterns As A Tool For Fire Danger Monitoring, Gabriele Caccamo, Laurie A. Chisholm, Ross A. Bradstock, Marjetta L. Puotinen

Faculty of Science - Papers (Archive)

Spatial connectivity of areas of dry fuels is considered a significant influence on the incidence of large fires. Precipitation patterns can dynamically affect fuel connectivity through controls on the distribution of dry fuels. Spatio-temporal monitoring of precipitation-driven variations in dry fuel connectivity patterns could therefore offer the potential to monitor fire danger. In this paper we present an innovative graph theoretic-based approach to monitor fire danger using remotely sensed patterns of dry fuel connectivity. We analysed the temporal evolution of dry fuel connectivity in south-eastern Australia during recent fire seasons. The analysis showed that rapid changes in the connectivity of …


Validation Of Iasi Forli Carbon Monoxide Retrievals Using Ftir Data From Ndacc, T Kerzenmacher, B Dils, N Kumps, T Blumenstock, C Clerbaux, P F. Coheur, P Demoulin, Omaira Garcia, M George, David W. Griffith, F Hase, J Hadji-Lazaro, D Hurtmans, Nicholas B. Jones, E Mahieu, Justus Notholt, Clare Paton-Walsh, U Raffalski, T Ridder, M Schneider, C Servais, M De Maziere Jan 2012

Validation Of Iasi Forli Carbon Monoxide Retrievals Using Ftir Data From Ndacc, T Kerzenmacher, B Dils, N Kumps, T Blumenstock, C Clerbaux, P F. Coheur, P Demoulin, Omaira Garcia, M George, David W. Griffith, F Hase, J Hadji-Lazaro, D Hurtmans, Nicholas B. Jones, E Mahieu, Justus Notholt, Clare Paton-Walsh, U Raffalski, T Ridder, M Schneider, C Servais, M De Maziere

Faculty of Science - Papers (Archive)

Carbon monoxide (CO) is retrieved daily and globally from space-borne IASI radiance spectra using the Fast Optimal Retrievals on Layers for IASI (FORLI) software developed at the Universit´e Libre de Bruxelles (ULB). The IASI CO total column product for 2008 from the most recent FORLI retrieval version (20100815) is evaluated using correlative CO profile products retrieved from groundbased solar absorption Fourier transform infrared (FTIR) observations at the following FTIR spectrometer sites from the Network for the Detection of Atmospheric Composition Change (NDACC): Ny-A° lesund, Kiruna, Bremen, Jungfraujoch, Iza˜na and Wollongong. In order to have good statistics for the comparisons, we …


Comparison Of Chemical Constituents And Antibacterial Activities And Antioxidant Activities Of The Essential Oil From Leaves And Fruits Of Bridelia Retusa (L.) A. Juss, D Sanseera, W Niwatananun, Saisunee Liawruangrath, Apiwat Baramee, Banyong Khantawa, Stephen G. Pyne, Boonsom Liawruangrath Jan 2012

Comparison Of Chemical Constituents And Antibacterial Activities And Antioxidant Activities Of The Essential Oil From Leaves And Fruits Of Bridelia Retusa (L.) A. Juss, D Sanseera, W Niwatananun, Saisunee Liawruangrath, Apiwat Baramee, Banyong Khantawa, Stephen G. Pyne, Boonsom Liawruangrath

Faculty of Science - Papers (Archive)

The essential oils from the leaves and fruits of Bridelia retusa (L.) A.Juss. were isolated by hydrodistillation. The essential oils were obtained in 0.0013% yield as a pale yellow liquid and 0.0026% yield as a violet-light brown liquid for the leaf oil and fruit oil respectively. The composition of each essential oil was analysed by means of GC-(FID) and GC-MS. Eleven constituents accounting for 48.77% of total leaves oil were identified. The most abundant compound was phytol (33.4%), followed by phthalic acid (5.2%), 6, 13-dimethoxy-2, 3, 9, 10-tetramethylpentacene-1, 4, 8, 11-tetrone (3.4%), heptacosane (2.3%) and nonacosane (1.2%). Sixteen constituents accounting …


Retention Of Different-Sized Particles And Derived Gut Fill Estimate In Tammar Wallabies (Macropus Eugenii): Physiological And Methodological Considerations, Adam J. Munn, Susie Tomlinson, Tom Savage, Marcus Clauss Jan 2012

Retention Of Different-Sized Particles And Derived Gut Fill Estimate In Tammar Wallabies (Macropus Eugenii): Physiological And Methodological Considerations, Adam J. Munn, Susie Tomlinson, Tom Savage, Marcus Clauss

Faculty of Science - Papers (Archive)

The capacity of the digestive tract is an important parameter in understanding digestive adaptations, particularly in herbivores. Measures of this capacity (‘gut fill’) are commonly performed in killed animals, which has ethical and logistical implications. Alternatively, dry matter gut contents (DMC) can be estimated in live animals from food intake, digesta retention and digestibility, based on physical principles (Holleman and White, Can. J. Zool. 67, 488–494, 1989). Although this method has been used to some extent, it still awaits thorough validation. Here we estimated DMC in seven tammar wallabies during 5-day feeding trials and compared the results to those gained …


The First Mitochondrial Genome For The Wasp Superfamily Platygastroidea: The Egg Parasitoid Trissolcus Basalis, Meng Mao, Alejandro Valerio, Andrew D. Austin, Mark Dowton, Norman F. Johnson Jan 2012

The First Mitochondrial Genome For The Wasp Superfamily Platygastroidea: The Egg Parasitoid Trissolcus Basalis, Meng Mao, Alejandro Valerio, Andrew D. Austin, Mark Dowton, Norman F. Johnson

Faculty of Science - Papers (Archive)

The nearly complete mitochondrial (mt) genome of an egg parasitoid, Trissolcus basalis (Wollaston), was sequenced using both 454 and Illumina next-generation sequencing technologies. A portion of the noncoding region remained unsequenced, possibly owing to the presence of repeats. The sequenced portion of the genome is 15 768 bp and has a high A+T content (84.2%), as is typical for hymenopteran mt genomes. A total of 36 of the 37 genes normally present in animal mt genomes were located. The one exception was trnR; a truncated version of this gene is present between trnS1 and nd5, but it is unclear whether …


Synchrotron Radiation Spectroscopic Techniques As Tools For The Medicinal Chemist: Microprobe X-Ray Fluorescence Imaging, X-Ray Absorption Spectroscopy, And Infrared Microspectroscopy, Carolyn Therese Dillon Jan 2012

Synchrotron Radiation Spectroscopic Techniques As Tools For The Medicinal Chemist: Microprobe X-Ray Fluorescence Imaging, X-Ray Absorption Spectroscopy, And Infrared Microspectroscopy, Carolyn Therese Dillon

Faculty of Science - Papers (Archive)

This review updates the recent advances and applications of three prominent synchrotron radiation techniques, microprobe X-ray fluorescence spectroscopy/imaging, X-ray absorption spectroscopy, and infrared microspectroscopy, and highlights how these tools are useful to the medicinal chemist. A brief description of the principles of the techniques is given with emphasis on the advantages of using synchrotron radiation-based instrumentation rather than instruments using typical laboratory radiation sources. This review focuses on several recent applications of these techniques to solve inorganic medicinal chemistry problems, focusing on studies of cellular uptake, distribution, and biotransformation of established and potential therapeutic agents. The importance of using these …


The Acos Co2 Retrieval Algorithm - Part Ii: Global Xco2 Data Characterization, D Crisp, B Fisher, C O'Dell, C Frankenberg, R Basilio, H Bosch, L R. Brown, R Castano, B Connor, N M. Deutscher, A Eldering, D Griffith, M Gunson, A Kuze, L Mandrake, J Mcduffie, J Messerschmidt, C E. Miller, I Morino, V Natraj, J Notholt, D M. O'Brien, F Oyafuso, I Polonsky, J Robinson, R Salawitch, V Sherlock, M Smyth, H Suto, T E. Taylor, D R. Thompson, P O. Wennberg, D Wunch, Y L. Yung Jan 2012

The Acos Co2 Retrieval Algorithm - Part Ii: Global Xco2 Data Characterization, D Crisp, B Fisher, C O'Dell, C Frankenberg, R Basilio, H Bosch, L R. Brown, R Castano, B Connor, N M. Deutscher, A Eldering, D Griffith, M Gunson, A Kuze, L Mandrake, J Mcduffie, J Messerschmidt, C E. Miller, I Morino, V Natraj, J Notholt, D M. O'Brien, F Oyafuso, I Polonsky, J Robinson, R Salawitch, V Sherlock, M Smyth, H Suto, T E. Taylor, D R. Thompson, P O. Wennberg, D Wunch, Y L. Yung

Faculty of Science - Papers (Archive)

Here, we report preliminary estimates of the column averaged carbon dioxide (CO2) dry air mole fraction, XCO2, retrieved from spectra recorded over land by the Greenhouse gases Observing Satellite, GOSAT (nicknamed "Ibuki"), using retrieval methods originally developed for the NASA Orbiting Carbon Observatory (OCO) mission. After screening for clouds and other known error sources, these retrievals reproduce much of the expected structure in the global XCO2 field, including its variation with latitude and season. However, low yields of retrieved XCO2 over persistently cloudy areas and ice covered surfaces at high latitudes limit the coverage of some geographic regions, even on …


Trends And Variations In Co, C2h6, And Hcn In The Southern Hemisphere Point To The Declining Anthropogenic Emissions Of Co And C2h6, G Zeng, S W. Wood, O Morgenstern, N B. Jones, J Robinson, D Smale Jan 2012

Trends And Variations In Co, C2h6, And Hcn In The Southern Hemisphere Point To The Declining Anthropogenic Emissions Of Co And C2h6, G Zeng, S W. Wood, O Morgenstern, N B. Jones, J Robinson, D Smale

Faculty of Science - Papers (Archive)

We analyse the carbon monoxide (CO), ethane (C2H6) and hydrogen cyanide (HCN) partial columns (from the ground to 12 km) derived from measurements by ground-based solar Fourier Transform Spectroscopy at Lauder, New Zealand (45° S, 170° E), and at Arrival Heights, Antarctica (78° S, 167° E), from 1997 to 2009. Significant negative trends are calculated for all species at both locations, based on the daily-mean observed time series, namely CO (−0.94 ± 0.47% yr−1), C2H6 (−2.37 ± 1.18% yr−1) and HCN (−0.93 ± 0.47% yr−1) at Lauder and CO (−0.92 ± 0.46% yr−1), C2H6 (−2.82 ± 1.37% yr−1) and HCN …


The First Climate Refugees? Contesting Global Narratives Of Climate Change In Tuvalu, Carol Farbotko, Heather Lazrus Jan 2012

The First Climate Refugees? Contesting Global Narratives Of Climate Change In Tuvalu, Carol Farbotko, Heather Lazrus

Faculty of Science - Papers (Archive)

Climate change effects such as sea-level rise are almost certain. What these outcomes mean for different populations, however, is far less certain. Climate change is both a narrative and material phenomenon. In so being, understanding climate change requires broad conceptualisations that incorporate multiple voices and recognise the agency of vulnerable populations. In climate change discourse, climate mobility is often characterised as the production of 'refugees', with a tendency to discount long histories of ordinary mobility among affected populations. The case of Tuvalu in the Pacific juxtaposes migration as everyday practice with climate refugee narratives. This climate-exposed population is being problematically …


Conservation Planning In A Cross-Cultural Context: The Wunambal Gaambera Healthy Country Project In The Kimberley, Western Australia, Heather Moorcroft, Emma Ignjic, Stuart Cowell, John Goonack, Sylvester Mangolomara, Janet Oobagooma, Regina Karadada, Dianna Williams, Neil Waina Jan 2012

Conservation Planning In A Cross-Cultural Context: The Wunambal Gaambera Healthy Country Project In The Kimberley, Western Australia, Heather Moorcroft, Emma Ignjic, Stuart Cowell, John Goonack, Sylvester Mangolomara, Janet Oobagooma, Regina Karadada, Dianna Williams, Neil Waina

Faculty of Science - Papers (Archive)

This article illustrates how a conservation planning approach combined Indigenous knowledge and Western science to support Indigenous Traditional Owners to make decisions about managing their ancestral lands and seas, and communicate more strategically with external stakeholders.


Architecture And Conservation Of The Bacterial Dna Replication Machinery, An Underexploited Drug Target, Andrew Robinson, Rebecca J. Causer, Nicholas E. Dixon Jan 2012

Architecture And Conservation Of The Bacterial Dna Replication Machinery, An Underexploited Drug Target, Andrew Robinson, Rebecca J. Causer, Nicholas E. Dixon

Faculty of Science - Papers (Archive)

"New antibiotics with novel modes of action are required to combat the growing threat posed by multi-drug resistant bacteria. Over the last decade, genome sequencing and other high-throughput techniques have provided tremendous insight into the molecular processes underlying cellular functions in a wide range of bacterial species. We can now use these data to assess the degree of conservation of certain aspects of bacterial physiology, to help choose the best cellular targets for development of new broad-spectrum antibacterials. DNA replication is a conserved and essential process, and the large number of proteins that interact to replicate DNA in bacteria are …