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Full-Text Articles in Social and Behavioral Sciences
Molecular Characterization Of A 21.4 Kilobase Antibiotic Resistance Plasmid From An Hemolytic Escherichia Coli O108:H-Human Clinical Isolate, Fay E. Dawes, Dieter M. Bulach, Alexander Kuzevski, Karl A. Bettelheim, Carola Venturini, Steven P. Djordjevic, Mark J. Walker
Molecular Characterization Of A 21.4 Kilobase Antibiotic Resistance Plasmid From An Hemolytic Escherichia Coli O108:H-Human Clinical Isolate, Fay E. Dawes, Dieter M. Bulach, Alexander Kuzevski, Karl A. Bettelheim, Carola Venturini, Steven P. Djordjevic, Mark J. Walker
Faculty of Science - Papers (Archive)
This study characterizes the 21.4 kilobase plasmid pECTm80 isolated from Escherichia coli strain 80, an α hemolytic human clinical diarrhoeal isolate (serotype O108:H-). DNA sequence analysis of pECTm80 revealed it belonged to incompatibility group X1, and contained plasmid partition and toxin-antitoxin systems, an R6K-like triple origin (ori) replication system, genes required for replication regulation, insertion sequences IS1R, ISEc37 and a truncated transposase gene (Tn3-like ΔtnpA) of the Tn3 family, and carried a class 2 integron. The class 2 integron of pECTm80 contains an intact cassette array dfrA1-sat2, encoding resistance to trimethoprim and streptothricin, …
Molecular Characterization Of Escherichia Coli Strains That Cause Symptomatic And Asymptomatic Urinary Tract Infections, Sam Abraham, Toni A. Chapman, Ren Zhang, James Chin, Amanda N. Mabbett, Makrina Totsika, Mark A. Schembri
Molecular Characterization Of Escherichia Coli Strains That Cause Symptomatic And Asymptomatic Urinary Tract Infections, Sam Abraham, Toni A. Chapman, Ren Zhang, James Chin, Amanda N. Mabbett, Makrina Totsika, Mark A. Schembri
Faculty of Science - Papers (Archive)
The differences between Escherichia coli strains associated with symptomatic and asymptomatic urinary tract infections (UTIs) remain to be properly determined. Here we examined the prevalence of plasmid types and bacteriocins, as well as genetic relatedness, in a defined collection of E. coli strains that cause UTIs. Comparative analysis identified a subgroup of strains with a high number of virulence genes (VGs) and microcins M/H47. We also identified associations between microcin genes, VGs, and specific plasmid types.
Molecular Characterization Of Commensal Escherichia Coli Adapted To Different Compartments Of The Porcine Gastrointestinal Tract, Sam Abraham, David M. Gordon, James Chin, Huub J. M Brouwers, Peter Njuguna, Mitchell D. Groves, Ren Zhang, Toni A. Chapman
Molecular Characterization Of Commensal Escherichia Coli Adapted To Different Compartments Of The Porcine Gastrointestinal Tract, Sam Abraham, David M. Gordon, James Chin, Huub J. M Brouwers, Peter Njuguna, Mitchell D. Groves, Ren Zhang, Toni A. Chapman
Faculty of Science - Papers (Archive)
The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n=146) isolated from different regions of the intestinal tracts of eight pigs (dueodenum, ileum, colon, and feces). The isolates were typed using the method of random amplified polymorphic DNA (RAPD) and screened for the presence of bacteriocin genes and plasmid replicon types. Molecular analysis of variance using the RAPD data showed that E. …
Characterization Of Gellan Gum By Capillary Electrophoresis, Danielle L. Taylor, Cameron J. Ferris, Alison R. Maniego, Patrice Castignolles, Marc In Het Panhuis, Marianne Gaborieau
Characterization Of Gellan Gum By Capillary Electrophoresis, Danielle L. Taylor, Cameron J. Ferris, Alison R. Maniego, Patrice Castignolles, Marc In Het Panhuis, Marianne Gaborieau
Faculty of Science - Papers (Archive)
Gellan gums were characterised for the first time using free-solution capillary electrophoresis (CE) or CE under critical conditions (CE-CC). CE-CC is a fast method that separates the polysaccharide. Gellan gums are shown to be heterogeneous in terms of their electrophoretic mobility at 55°C revealing: oligomer peak(s), broad peaks of polymers with a random coil conformation with different degrees of acylation (composition), aggregates, and polymers with double-helix conformation. CE-CC is complementary with the rheological analysis also performed in this work. Sonication of gellan gums is shown to decrease the viscosity of gellan gum mainly by breaking up aggregates. The effect of …
The Acos Co2 Retrieval Algorithm - Part Ii: Global Xco2 Data Characterization, D Crisp, B Fisher, C O'Dell, C Frankenberg, R Basilio, H Bosch, L R. Brown, R Castano, B Connor, N M. Deutscher, A Eldering, D Griffith, M Gunson, A Kuze, L Mandrake, J Mcduffie, J Messerschmidt, C E. Miller, I Morino, V Natraj, J Notholt, D M. O'Brien, F Oyafuso, I Polonsky, J Robinson, R Salawitch, V Sherlock, M Smyth, H Suto, T E. Taylor, D R. Thompson, P O. Wennberg, D Wunch, Y L. Yung
The Acos Co2 Retrieval Algorithm - Part Ii: Global Xco2 Data Characterization, D Crisp, B Fisher, C O'Dell, C Frankenberg, R Basilio, H Bosch, L R. Brown, R Castano, B Connor, N M. Deutscher, A Eldering, D Griffith, M Gunson, A Kuze, L Mandrake, J Mcduffie, J Messerschmidt, C E. Miller, I Morino, V Natraj, J Notholt, D M. O'Brien, F Oyafuso, I Polonsky, J Robinson, R Salawitch, V Sherlock, M Smyth, H Suto, T E. Taylor, D R. Thompson, P O. Wennberg, D Wunch, Y L. Yung
Faculty of Science - Papers (Archive)
Here, we report preliminary estimates of the column averaged carbon dioxide (CO2) dry air mole fraction, XCO2, retrieved from spectra recorded over land by the Greenhouse gases Observing Satellite, GOSAT (nicknamed "Ibuki"), using retrieval methods originally developed for the NASA Orbiting Carbon Observatory (OCO) mission. After screening for clouds and other known error sources, these retrievals reproduce much of the expected structure in the global XCO2 field, including its variation with latitude and season. However, low yields of retrieved XCO2 over persistently cloudy areas and ice covered surfaces at high latitudes limit the coverage of some geographic regions, even on …