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Articles 61 - 90 of 892
Full-Text Articles in Optics
Study Of Single-Photon Wave-Packets With Atomically Thin Nonlinear Mirrors, Christopher Klenke
Study Of Single-Photon Wave-Packets With Atomically Thin Nonlinear Mirrors, Christopher Klenke
Graduate Theses and Dissertations
A novel controlled phase gate for photonic quantum computing is proposed by exploiting the powerful nonlinear optical responses of atomically thin transition metal dichalcogenides (TMDs) and it is shown that such a gate could elicit a π-rad phase shift in the outgoing electric field only in the case of two incident photons and no other cases. Firstly, the motivation for such a gate is developed and then the implementation of monolayer TMDs is presented as a solution to previous realization challenges. The single-mode case of incident photons upon a TMD is derived and is then used to constrain the more …
Subwavelength Engineering Of Silicon Photonic Waveguides, Farhan Bin Tarik
Subwavelength Engineering Of Silicon Photonic Waveguides, Farhan Bin Tarik
All Dissertations
The dissertation demonstrates subwavelength engineering of silicon photonic waveguides in the form of two different structures or avenues: (i) a novel ultra-low mode area v-groove waveguide to enhance light-matter interaction; and (ii) a nanoscale sidewall crystalline grating performed as physical unclonable function to achieve hardware and information security. With the advancement of modern technology and modern supply chain throughout the globe, silicon photonics is set to lead the global semiconductor foundries, thanks to its abundance in nature and a mature and well-established industry. Since, the silicon waveguide is the heart of silicon photonics, it can be considered as the core …
Noncontact Liquid Crystalline Broadband Optoacoustic Sensors, Hengky Chandrahalim, Michael T. Dela Cruz
Noncontact Liquid Crystalline Broadband Optoacoustic Sensors, Hengky Chandrahalim, Michael T. Dela Cruz
AFIT Patents
An optoacoustic sensor includes a liquid crystal (LC) cell formed between top and bottom plates of transparent material. A transverse grating formed across the LC cell that forms an optical transmission bandgap. A CL is aligned to form a spring-like, tunable Bragg grating that is naturally responsive to external agitations providing a spectral transition regime, or edge, in the optical transmission bandgap of the transverse grating that respond to broadband acoustic waves. The optoacoustic sensor includes a narrowband light source that is oriented to transmit light through the top plate, the LC cell, and the bottom plate. The optoacoustic sensor …
Imnets: Deep Learning Using An Incremental Modular Network Synthesis Approach For Medical Imaging Applications, Redha A. Ali, Russell C. Hardie, Barath Narayanan Narayanan, Temesguen Messay
Imnets: Deep Learning Using An Incremental Modular Network Synthesis Approach For Medical Imaging Applications, Redha A. Ali, Russell C. Hardie, Barath Narayanan Narayanan, Temesguen Messay
Electrical and Computer Engineering Faculty Publications
Deep learning approaches play a crucial role in computer-aided diagnosis systems to support clinical decision-making. However, developing such automated solutions is challenging due to the limited availability of annotated medical data. In this study, we proposed a novel and computationally efficient deep learning approach to leverage small data for learning generalizable and domain invariant representations in different medical imaging applications such as malaria, diabetic retinopathy, and tuberculosis. We refer to our approach as Incremental Modular Network Synthesis (IMNS), and the resulting CNNs as Incremental Modular Networks (IMNets). Our IMNS approach is to use small network modules that we call SubNets …
Maximum Trapping Focal Length In Photophoretic Trap For 3d Imaging Systems, Jason M. Childers
Maximum Trapping Focal Length In Photophoretic Trap For 3d Imaging Systems, Jason M. Childers
Electrical Engineering
This product is a photophoretic trapping system which allows varying focal lengths to test which focal lengths are possible for trapping toner particles. This system establishes that there exists a maximum trapping distance limitation and is the first time the effect of focal length is studied in a photophoretic trapping system. Increasing photophoretic trapping focal length is necessary for improving this technology as a 3D display. The 3D imaging technology is realized by dragging a microscopic (micrometer-scale) particles with a laser beam to trace an image. This technology can display fully colored and high-resolution 3D images visible from almost any …
Methods For Focal Plane Array Resolution Estimation Using Random Laser Speckle In Non-Paraxial Geometries, Phillip J. Plummer
Methods For Focal Plane Array Resolution Estimation Using Random Laser Speckle In Non-Paraxial Geometries, Phillip J. Plummer
Theses and Dissertations
The infrared (IR) imaging community has a need for direct IR detector evaluation due to the continued demand for small pixel pitch detectors, the emergence of strained-layer-super-lattice devices, and the associated lateral carrier diffusion issues. Conventional laser speckle-based modulation transfer function (MTF) estimation is dependent on Fresnel propagation and a wide-sense-stationary input random process, limiting the use of this approach for lambda (wavelength)-scale IR devices. This dissertation develops two alternative methodologies for speckle-based resolution evaluation of IR focal plane arrays (FPAs). Both techniques are formulated using Rayleigh-Sommerfield electric field propagation, making them valid in the non-paraxial geometries dictated for resolution …
Hinged Temperature-Immune Self-Referencing Fabry–Pérot Cavity Sensors, Jeremiah C. Williams, Hengky Chandrahalim
Hinged Temperature-Immune Self-Referencing Fabry–Pérot Cavity Sensors, Jeremiah C. Williams, Hengky Chandrahalim
AFIT Patents
A passive microscopic Fabry-Pérot Interferometer (FPI) sensor includes a three-dimensional microscopic optical structure formed on a cleaved tip of the optical fighter using a two-photon polymerization process on a photosensitive polymer by a three-dimensional micromachining device. The three-dimensional microscopic optical structure having a hinged optical layer pivotally connected to a distal portion of a suspended structure. A reflective layer is deposited on a mirror surface of the hinged optical layer while in an open position. The hinged optical layer is subsequently positioned in the closed position to align the mirror surface to at least partially reflect a light signal back …
Porous Silicon Photonics For Label-Free Interferometric Biosensing And Flat Optics, Tahmid Hassan Talukdar
Porous Silicon Photonics For Label-Free Interferometric Biosensing And Flat Optics, Tahmid Hassan Talukdar
All Dissertations
This dissertation uses porous silicon as a material platform to explore novel optical effects in three domains: (i) It studies dispersion engineering in integrated waveguides to achieve high performance group index sensing. With proper design parameters, the sensor waveguides can theoretically achieve 6 times larger group index shift compared to the actual bulk effective refractive index shift. We demonstrate the guided mode confinement factor to be a key parameter in design and implementation of these waveguides. (ii) It explores multicolor laser illumination to experimentally demonstrate perceptually enhanced colorimetric sensing, overcoming the limitations faced by many contemporary colorimetric sensors. Our technique …
Microscopic Nuclei Classification, Segmentation, And Detection With Improved Deep Convolutional Neural Networks (Dcnn), Md Zahangir Alom, Vijayan K. Asari, Anil Parwani, Tarek M. Taha
Microscopic Nuclei Classification, Segmentation, And Detection With Improved Deep Convolutional Neural Networks (Dcnn), Md Zahangir Alom, Vijayan K. Asari, Anil Parwani, Tarek M. Taha
Electrical and Computer Engineering Faculty Publications
Background Nuclei classification, segmentation, and detection from pathological images are challenging tasks due to cellular heterogeneity in the Whole Slide Images (WSI). Methods In this work, we propose advanced DCNN models for nuclei classification, segmentation, and detection tasks. The Densely Connected Neural Network (DCNN) and Densely Connected Recurrent Convolutional Network (DCRN) models are applied for the nuclei classification tasks. The Recurrent Residual U-Net (R2U-Net) and the R2UNet-based regression model named the University of Dayton Net (UD-Net) are applied for nuclei segmentation and detection tasks respectively. The experiments are conducted on publicly available datasets, including Routine Colon Cancer (RCC) classification and …
Towards Improved Inertial Navigation By Reducing Errors Using Deep Learning Methodology, Hua Chen, Tarek M. Taha, Vamsy P. Chodavarapu
Towards Improved Inertial Navigation By Reducing Errors Using Deep Learning Methodology, Hua Chen, Tarek M. Taha, Vamsy P. Chodavarapu
Electrical and Computer Engineering Faculty Publications
Autonomous vehicles make use of an Inertial Navigation System (INS) as part of vehicular sensor fusion in many situations including GPS-denied environments such as dense urban places, multi-level parking structures, and areas with thick tree-coverage. The INS unit incorporates an Inertial Measurement Unit (IMU) to process the linear acceleration and angular velocity data to obtain orientation, position, and velocity information using mechanization equations. In this work, we describe a novel deep-learning-based methodology, using Convolutional Neural Networks (CNN), to reduce errors from MEMS IMU sensors. We develop a CNN-based approach that can learn from the responses of a particular inertial sensor …
Finding Signal In The Noise: High-Fidelity, Quantitative, Optical Blood Perfusion Imaging With Interference, Abdul Mohaimen Safi
Finding Signal In The Noise: High-Fidelity, Quantitative, Optical Blood Perfusion Imaging With Interference, Abdul Mohaimen Safi
USF Tampa Graduate Theses and Dissertations
For label-free, non-invasive, wide field-of-view (FOV) imaging/monitoring of blood flow, speckle-based approaches are gaining popularity. However, to obtain quantitative flow information, speckle techniques rely on the multi-exposure scheme which requires complex, bulky, and expensive instrumentation, limiting its application to preclinical studies. This dissertation directly addresses these issues. In the first part of this dissertation, we report a novel single shot synthetic multi-exposure speckle imaging (syMESI) method to synthetically produce multi-exposure images from one short single exposure speckle image using spatial binning/averaging. We demonstrate that syMESI can reimagine conventional hardware based MESI, with low-cost single exposure laser speckle imaging (LSCI) instrumentation. …
Method Of Making Hinged Self-Referencing Fabry–Pérot Cavity Sensors, Jeremiah C. Williams, Hengky Chandrahalim
Method Of Making Hinged Self-Referencing Fabry–Pérot Cavity Sensors, Jeremiah C. Williams, Hengky Chandrahalim
AFIT Patents
A method is provided for fabricating a passive optical sensor on a tip of an optical fiber. The method includes perpendicularly cleaving a tip of an optical fiber and mounting the tip of the optical fiber in a specimen holder of a photosensitive polymer three-dimensional micromachining machine. The method includes forming a three-dimensional microscopic optical structure within the photosensitive polymer that comprises a two cavity Fabry-Perot Interferometer (FPI) having a hinged optical layer that is pivotally coupled to a suspended structure. The method includes removing an uncured portion of the photosensitive polymer using a solvent. The method includes depositing a …
Three Wave Mixing In Epsilon-Near-Zero Plasmonic Waveguides For Signal Regeneration, Nicholas Mirchandani, Mark C. Harrison
Three Wave Mixing In Epsilon-Near-Zero Plasmonic Waveguides For Signal Regeneration, Nicholas Mirchandani, Mark C. Harrison
Engineering Faculty Articles and Research
Vast improvements in communications technology are possible if the conversion of digital information from optical to electric and back can be removed. Plasmonic devices offer one solution due to optical computing’s potential for increased bandwidth, which would enable increased throughput and enhanced security. Plasmonic devices have small footprints and interface with electronics easily, but these potential improvements are offset by the large device footprints of conventional signal regeneration schemes, since surface plasmon polaritons (SPPs) are incredibly lossy. As such, there is a need for novel regeneration schemes. The continuous, uniform, and unambiguous digital information encoding method is phase-shift-keying (PSK), so …
Resolving The Three-Dimensional Rotational And Translational Dynamics Of Single Molecules Using Radially And Azimuthally Polarized Fluorescence, Oumeng Zhang, Weiyan Zhou, Jin Lu, Tingting Wu, Matthew D. Lew
Resolving The Three-Dimensional Rotational And Translational Dynamics Of Single Molecules Using Radially And Azimuthally Polarized Fluorescence, Oumeng Zhang, Weiyan Zhou, Jin Lu, Tingting Wu, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
We report a radially and azimuthally polarized (raPol) microscope for high detection and estimation performance in single-molecule orientation-localization microscopy (SMOLM). With 5000 photons detected from Nile red (NR) transiently bound within supported lipid bilayers (SLBs), raPol SMOLM achieves 2.9 nm localization precision, 1.5° orientation precision, and 0.17 sr precision in estimating rotational wobble. Within DPPC SLBs, SMOLM imaging reveals the existence of randomly oriented binding pockets that prevent NR from freely exploring all orientations. Treating the SLBs with cholesterol-loaded methyl-β-cyclodextrin (MβCD-chol) causes NR’s orientational diffusion to be dramatically reduced, but curiously NR’s median lateral displacements drastically increase from 20.8 to …
A Deep Neural Network For Early Detection And Prediction Of Chronic Kidney Disease, Vijendra Singh, Vijayan K. Asari, Rajkumar Rajasekaran
A Deep Neural Network For Early Detection And Prediction Of Chronic Kidney Disease, Vijendra Singh, Vijayan K. Asari, Rajkumar Rajasekaran
Electrical and Computer Engineering Faculty Publications
Diabetes and high blood pressure are the primary causes of Chronic Kidney Disease (CKD). Glomerular Filtration Rate (GFR) and kidney damage markers are used by researchers around the world to identify CKD as a condition that leads to reduced renal function over time. A person with CKD has a higher chance of dying young. Doctors face a difficult task in diagnosing the different diseases linked to CKD at an early stage in order to prevent the disease. This research presents a novel deep learning model for the early detection and prediction of CKD. This research objectives to create a deep …
Enhanced Study Of Complex Systems By Unveiling Hidden Symmetries With Dynamical Visibility, Nhat Vu Minh Nguyen
Enhanced Study Of Complex Systems By Unveiling Hidden Symmetries With Dynamical Visibility, Nhat Vu Minh Nguyen
2022 Symposium
One of the great challenges in complex and chaotic dynamics is to reveal its deterministic structures. These temporal dynamical structures are sometimes a consequence of hidden symmetries. Detecting and understanding them can allow the study of complex systems even without knowing the full underlying mathematical description of the system. Here we introduce a new technique, called Dynamical Visibility, that quantifies temporal correlations of the dynamics based upon some symmetry conditions. This visibility measures the departure of the dynamics from internal symmetries. We apply this technique to well-known chaotic systems, such as the logistic map and the circle map, as well …
A Progressive Learning Strategy For Large-Scale Glacier Mapping, Zhiyuan Xie, Umesh K. Haritashya, Vijayan K. Asari
A Progressive Learning Strategy For Large-Scale Glacier Mapping, Zhiyuan Xie, Umesh K. Haritashya, Vijayan K. Asari
Electrical and Computer Engineering Faculty Publications
In recent years, the worldwide temperature increase has resulted in rapid deglaciation and a higher risk of glacier-related natural hazards such as flooding and debris flow. Due to the severity of these hazards, continuous observation and detailed analysis of glacier fluctuations are crucial. Many such analyses require an accurately delineated glacier boundary. However, the complexity and heterogeneity of glaciers, particularly debris-covered glaciers (DCGs), poses a challenge for glacier mapping when using conventional remote sensing or machine-learning techniques. Some examples exist about small-scale automated glacier mapping, but large or regional-scale mapping is challenging. Previously, a deep-learning-based approach named GlacierNet2 had been …
Patterned Liquid Crystal Devices For Near-Eye Displays, Kun Yin
Patterned Liquid Crystal Devices For Near-Eye Displays, Kun Yin
Electronic Theses and Dissertations, 2020-2023
As a promising next-generation display, augmented reality (AR) and virtual reality (VR) have shown attractive features and attracted broad interests from both academia and industry. Currently, these near-eye displays (NEDs) have enabled numerous applications, ranging from education, medical, entertainment, to engineering, with the help of compact and functional patterned liquid crystal (LC) devices. The interplay between LC patterns and NEDs stimulates the development of novel LC devices with unique surface alignments and volume structures, which in turn feedback to achieve more compact and versatile NEDs. This dissertation will focus on the patterned LC with applications in NEDs. Firstly, we propose …
Diffractive Liquid Crystal Optical Elements For Near-Eye Displays, Jianghao Xiong
Diffractive Liquid Crystal Optical Elements For Near-Eye Displays, Jianghao Xiong
Electronic Theses and Dissertations, 2020-2023
Liquid crystal planar optics (LCPO) with versatile functionalities is emerging as a promising candidate for overcoming various challenges in near-eye displays, like augmented reality (AR) and virtual reality (VR), while maintaining a small form factor. This type of novel optical element exhibits unique properties, such as high efficiency, large angular/spectral bandwidths, polarization selectivity, and dynamic modulation. The basic molecular configuration of these novel reflective LCPO is analyzed, based on the simulation of molecular dynamics. In contrast to previously assumed planar-twist structure, our analysis predicts a slanted helix structure, which agrees with the measured results. The optical simulation model is established …
Development Of Holographic Phase Masks For Wavefront Shaping, Nafiseh Mohammadian
Development Of Holographic Phase Masks For Wavefront Shaping, Nafiseh Mohammadian
Electronic Theses and Dissertations, 2020-2023
This dissertation explores a new method for creating holographic phase masks (HPMs), which are phase transforming optical elements holographically recorded in photosensitive glass. This novel hologram recording method allows for the fast production of HPMs of any complexity, as opposed to the traditional multistep process, which includes the design and fabrication of a master phase mask operating in the UV region before the holographic recording step. We holographically recorded transmissive HPMs that are physically robust (they are recorded in a silicate glass volume), can handle tens of kilowatts of continuous wave (CW) laser power, are un-erasable, user defined, require no …
Meltpondnet: A Swin Transformer U-Net For Detection Of Melt Ponds On Arctic Sea Ice, Ivan Sudakow, Vijayan K. Asari, Ruixu Liu, Denis Demchev
Meltpondnet: A Swin Transformer U-Net For Detection Of Melt Ponds On Arctic Sea Ice, Ivan Sudakow, Vijayan K. Asari, Ruixu Liu, Denis Demchev
Electrical and Computer Engineering Faculty Publications
High-resolution aerial photographs of Arctic region are a great source for different sea ice feature recognition, which are crucial to validate, tune, and improve climate models. Melt ponds on the surface of melting Arctic sea ice are of particular interest as they are sensitive and valuable indicators and are proxy to the processes in the Arctic climate system. Manual analysis of this remote sensing data is extremely difficult and time-consuming due to the complex shapes and unpredictable boundaries of the melt ponds, and that leads to the necessity for automatizing the processes. In this study, we propose a robust and …
Circuit Optimization Techniques For Efficient Ex-Situ Training Of Robust Memristor Based Liquid State Machine, Alex Henderson, Christopher Yakopcic, Cory Merkel, Steven Harbour, Tarek M. Taha, Hananel Hazan
Circuit Optimization Techniques For Efficient Ex-Situ Training Of Robust Memristor Based Liquid State Machine, Alex Henderson, Christopher Yakopcic, Cory Merkel, Steven Harbour, Tarek M. Taha, Hananel Hazan
Electrical and Computer Engineering Faculty Publications
Spiking neural network hardware offers a high performance, power-efficient and robust platform for the processing of complex data. Many of these systems require supervised learning, which poses a challenge when using gradient-based algorithms due to the discontinuous properties of SNNs. Memristor based hardware can offer gains in portability, power reduction, and throughput efficiency when compared to pure CMOS. This paper proposes a memristor-based spiking liquid state machine (LSM). The inherent dynamics of the LSM permit the use of supervised learning without backpropagation for weight updates. To carry out the design space evaluation of the LSM for optimal hardware performance, several …
Optical Signal Processing With Discrete-Space Metamaterials, Mohammad Moein Moeini
Optical Signal Processing With Discrete-Space Metamaterials, Mohammad Moein Moeini
Wayne State University Dissertations
As digital circuits are approaching the limits of Moore’s law, a great deal of efforthas been directed to alternative computing approaches. Among them, the old concept of optical signal processing (OSP) has attracted attention, revisited in the light of metamaterials and nano-photonics. This approach has been successful in realizing basic mathematical operations, such as derivatives and integrals, but it is difficult to be applied to more complex ones. Inspired by digital filters, we propose a radically new OSP approach, able to realize arbitrary mathematical operations over a nano-photonic platform. We demonstrate this concept for the case of spatial differentiation, image …
Light Trapping Transparent Electrodes, Mengdi Sun
Light Trapping Transparent Electrodes, Mengdi Sun
Electronic Theses and Dissertations, 2020-2023
Transparent electrodes represent a critical component in a wide range of optoelectronic devices such as high-speed photodetectors and solar cells. Fundamentally, the presence of any conductive structures in the optical path leads to dissipation and reflection, which adversely affects device performance. Many different approaches have been attempted to minimize such shadowing losses, including the use of transparent conductive oxides (TCOs), metallic nanowire mesh grids, graphene-based contacts, and high-aspect ratio metallic wire arrays. In this dissertation I discuss a conceptually different approach to achieve transparent electrodes, which involves recapturing photons initially reflected by highly conductive electrode lines. To achieve this, light-redirecting …
Temperature-Immune Self-Referencing Fabry–Pérot Cavity Sensors, Hengky Chandrahalim, Jonathan W. Smith
Temperature-Immune Self-Referencing Fabry–Pérot Cavity Sensors, Hengky Chandrahalim, Jonathan W. Smith
AFIT Patents
A passive microscopic Fabry-Pérot Interferometer (FPI) sensor an optical fiber a three-dimensional microscopic optical structure formed on a cleaved tip of an optical fighter that reflects a light signal back through the optical fiber. The reflected light is altered by refractive index changes in the three-dimensional structure that is subject to at least one of: (i) thermal radiation; and (ii) volatile organic compounds.
Intracavity Phase Interferometry Based Fiber Sensors, Luke Jameson Horstman
Intracavity Phase Interferometry Based Fiber Sensors, Luke Jameson Horstman
Optical Science and Engineering ETDs
Intracavity Phase Interferometry (IPI) is a detection technique that exploits the inherent sensitivity of a laser's frequency to the parameters of its cavity. Intracavity interferometry is orders of magnitude more sensitive than its extracavity alternatives. This dissertation improves on previous free-space proof-of-concept designs. By implementing the technique in fiber optics, using optical parametric oscillation, and investigating non-Hermitian quantum mechanics and dispersion tailoring enhancement techniques, IPI has become more applicable and sensitive. Ring and linear IPI configurations were realized in this work, both operating as bidirectional fiber optical parametric oscillators. The benefit of using externally pumped synchronous optical parametric oscillation is …
Spectral Dependence Of Deep Subwavelength Metallic Apertures In The Mid-Wave Infrared, Heath Gemar
Spectral Dependence Of Deep Subwavelength Metallic Apertures In The Mid-Wave Infrared, Heath Gemar
Electronic Theses and Dissertations, 2020-2023
For two decades, extraordinary optical transmission (EOT) has amplified exploration into subwavelength systems. Researchers have previously suggested exploiting the spectrally selective electromagnetic field confinement of subwavelength cavities for multispectral detectors. Utilizing the finite-difference frequency domain (FDFD) method, we examine electromagnetic field confinement in both 2-dimensional and 3-dimensional scenarios from 2.5 to 6 microns (i.e., mid-wave infrared or MWIR). We explore the trade space of deep subwavelength cavities and its impact on resonant enhancement of the electromagnetic field. The studies provide fundamental understanding of the coupling mechanisms allowing for prediction of resonant spectral behavior based on cavity geometry and material properties. …
Evaluating Deep-Learning Models For Debris-Covered Glacier Mapping, Zhiyuan Xie, Vijayan K. Asari, Umesh K. Haritashya
Evaluating Deep-Learning Models For Debris-Covered Glacier Mapping, Zhiyuan Xie, Vijayan K. Asari, Umesh K. Haritashya
Electrical and Computer Engineering Faculty Publications
In recent decades, mountain glaciers have experienced the impact of climate change in the form of accelerated glacier retreat and other glacier-related hazards such as mass wasting and glacier lake outburst floods. Since there are wide-ranging societal consequences of glacier retreat and hazards, monitoring these glaciers as accurately and repeatedly as possible is important. However, the accurate glacier boundary, especially the debriscovered glacier (DCG) boundary, which is one of the primary inputs in many glacier analyses, remains a challenge even after many years of research using conventional remote sensing methods or machine-learning methods. The GlacierNet, a deep-learning-based approach, utilized the …
Fabricating Nanophotonic Devices Using Nanofabrication Techniques, Scott Cummings
Fabricating Nanophotonic Devices Using Nanofabrication Techniques, Scott Cummings
Student Scholar Symposium Abstracts and Posters
Nanofabrication processes are widely used to make the integrated circuits and computer chips that are ubiquitous in today’s technology. These fabrication processes can also be applied to the creation of nanophotonic devices. The ways in which we apply these fabrication techniques in the field of photonics is often constrained by the technologies used for electronics manufacturing which presents an interesting engineering challenge. These limitations include availability and cost of certain fabrication equipment and techniques required to create state-of-the-art nanophotonic devices. Through work with the University of California Irvine nano-fabrication cleanroom, we designed and fabricated various integrated photonic components including grating …
Single-Molecule Localization Microscopy Of 3d Orientation And Anisotropic Wobble Using A Polarized Vortex Point Spread Function, Tianben Ding, Matthew D. Lew
Single-Molecule Localization Microscopy Of 3d Orientation And Anisotropic Wobble Using A Polarized Vortex Point Spread Function, Tianben Ding, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
Within condensed matter, single fluorophores are sensitive probes of their chemical environments, but it is difficult to use their limited photon budget to image precisely their positions, 3D orientations, and rotational diffusion simultaneously. We demonstrate the polarized vortex point spread function (PSF) for measuring these parameters, including characterizing the anisotropy of a molecule’s wobble, simultaneously from a single image. Even when imaging dim emitters (∼500 photons detected), the polarized vortex PSF can obtain 12 nm localization precision, 4°–8° orientation precision, and 26° wobble precision. We use the vortex PSF to measure the emission anisotropy of fluorescent beads, the wobble dynamics …