Chemistry Commons^™

The Isolation And Identification Of Polyphenolics From Grape Seeds And Their Activity Towards Specific Cell Lines, Joseph A. Reddy

Chemistry & Biochemistry Theses & Dissertations

Grape seed extract is being marketed in France for cardiovascular use and skin care. The active constituents of both these drugs are certain polyphenols called procyanidins. A similar seed extract has now been obtained from Virginia Chardonnay grape seeds. Ten of the major components in this extract have been isolated in their pure form by the semi-preparative HPLC separation on a YMC ODS-AQ (Octadecylsilane-AQ) column. The ODS-Aq column has been shown to have a better resolving capacity compared to the Spherisorb ODS (Octadecylsilane) column previously described in the literature. This has been attributed to the ODSAQ support which has both …

The Cytotoxic Effect Of Tropical Plants On Human And Rat Hepatoma Cells, Mark Tafazoli

Honors Capstone Projects and Theses

No abstract provided.

Investigation Of The Structural Heterogeneity Of Lipooligosaccharides From Pathogenic Haemophilus And Neisseria Species And Of R-Type Lipopolysaccharides From Salmonella Typhimurium By Electrospray Mass Spectrometry, B W. Gibson, William Melaugh, Nancy J. Phillips, M A. Apicella, A A. Campagnari, J M. Griffiss

Chemistry Faculty Publications

Heterogeneity in the lipooligosaccharides (LOS) of pathogenic Haemophilus and Neisseria species is evident from the multiplicity of components observed with electrophoretic analyses. Knowledge of the precise structures that make up these diverse LOS molecules is clearly the key to reaching an understanding of pathogenic processes such as phase variation and molecular mimicry. Except for a few cases, little is known about the specific structural features of LOS that underlie phase variation and molecular mimicry, partly because of the inherent difficulties in the structural elucidation of these complex glycolipids. In the lipopolysaccharides (LPS) from Salmonella typhimurium and Escherichia coli, rough, or …

A Review Of The Biology Of Giant Salvinia, J. Douglas Oliver

Faculty Publications and Presentations

Giant salvinia (Salviniaceae) is a potentially serious aquatic weed that is native to Brazil. It has been reported in more than 20 countries, but is not established in the U.S. at this time. Mitchell and Tur (1975) reported that three years after the formation of the Kariba Reservoir in Africa, giant salvinia blanketed 21.5% or 1003 km2 of the reservoir surface area. Creagh (199111992) wrote, "A single small plant may grow to form a thick mat covering more than 100 sq. km. in just three months - choking lakes and waterways, reducing populations of aquatic plants and animals and in …

A Comparative 2-Dimensional Gel Protein Database Of The Intact And Regenerating Newt Limbs, Panagiotis A. Tsonis

Biology Faculty Publications

In this paper we describe a two-dimensional gel database of the regenerating newt limb. Protein synthesis was compared in the intact limb, in the 1-week regenerating limb, representing the dedifferentiation stage, and in the 2-week regenerating limb, representing the formation of the blastema. This comparative database provided data on differential expression of about 800 proteins during the process of limb regeneration. In addition, a map has been generated for these proteins for future guidance in characterizing further new, unknown proteins. The overall expression patterns of the proteins indicated that the dedifferentiation stage was marked by down-regulation of most proteins, while …

Identification Of The Nd Δ And Σ States And The 1,3Φ←←X  3Σ− G Transition Of O2 By Resonant Multiphoton Ionization, Robert J. Yokelson, R. J. Lipert, W. A. Chupka

Chemistry and Biochemistry Faculty Publications

Spectra of the 3dRydberg state region of O₂ have been obtained by two‐photon resonant ionization of the ground electronic state. By varying the rotational distribution and radiation polarization, all observed bands were identified and attributed to excitation of Σ, Δ, and Φ states. Earlier assignments were corrected. The Δ and Φ assignments are complete while the Σ assignments are so far incomplete.

Identification Of The Ns And Nd Rydberg States Of O2 For N=3–5, Robert J. Yokelson, R. J. Lipert, W. A. Chupka

Chemistry and Biochemistry Faculty Publications

The 4s‐3d and 5s‐4dRydberg complexes of diatomic oxygen have been studied by (2+1) resonance‐enhanced multiphoton ionization of the X  ³∑ _g ⁻ ground state of O₂. We have located and identified at least two vibrational levels of each of the following states: Three of four expected 4sσ Π states; all four expected 5sσ Π states; 18 of 22 expected 3d states (with only the states of the 3dσ orbital remaining unobserved); and 5 of the 10 predicted 4dπ states. State assignments …

Evidence For Helical Structures In Poly(1-Olefin Sulfones) By Transmission Electron Microscopy, George C. Ruben, W H. Stockmayer

Dartmouth Scholarship

Transmission electron microscope images were obtained of fractions of poly(1-tetradecene sulfone) and poly(cyclohexene sulfone) cast from very dilute solutions (0.007%, wt/vol) and rapidly freeze-dried on a mica surface. The samples were then vertically platinum-carbon (Pt-C) replicated with 9 +/- 0.3-A Pt-C and held together with 128 A of electron-transparent evaporated carbon. The Pt-C coating enlarges the molecular chain diameters by approximately 5 A, so that a single polysulfone chain has an apparent diameter of 9-12 A in the transmission electron microscope. Poly(1-tetradecene sulfone) forms short helical regions that show irregular helical turns of pitch 7-18 A, two to eight turns …

Mobilization Of Iron Enhances The Iron-Dependent Biochemical Reactivity Of Asbestos In Vitro And Contributes To The Cytotoxicity Of Asbestos In Cultured Cells, Loren Glen Lund

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Asbestos related research began approximately 60 years ago, yet, the mechanism(s) by which asbestos exerts its biological effects is not well understood. The hypothesis upon which this dissertation is based is that mobilization of iron from asbestos enhances the iron-dependent biochemical reactivity of asbestos in vitro and contributes to asbestos-dependent cytotoxicity. The specific aims for this hypothesis were, 1) to determine whether iron was responsible for the biochemical reactivity of asbestos in vitro and asbestos-induced cytotoxicity in cultured cells, and 2) to determine whether mobilization of iron from asbestos enhanced the reactions catalyzed by asbestos in vitro and contributes to …

Thermal Properties Of Some Lipid Components Of Cell Membranes, Lihua Wei

Masters Theses & Specialist Projects

Phospholipids are the major structural components of the lipid portion of biological membranes. The study of the thermal properties of these phospholipid provides a systematic understanding of the relationships between the chemical structures and functional properties of phospholipid molecules in artificial and biological membranes. This study examined the thermal behavior of phospholipids in the solid state using DSC and TG coupled with FITR. The phospholipids were found to have phase transitions below the melting point. Egg-yolk lecithin had four transitions below its melting point; sphingomyelin had two phase transitions below its melting point. Phosphatidylethanolamine and phosphatidylserine had complex phase transitions. …

Biodegradation Of Crude Oil By Marine Higher Fungi, Brian J. Dyer

Biological Sciences Theses & Dissertations

Four experiments were performed to determine the ability of marine higher fungi to grow in and degrade crude oil. Eight species, previously determined to be hydrocarbonoclastic, were grown in artificial sea water medium (ASM) and southern Louisiana crude oil. The dry mycelial weights were measured and consumption of crude oil was measured gravimetrically. The two species with the best growth, Corollospora maritima and Zalerion maritimum, were grown in ASM containing crude oil spiked with three known hydrocarbons. To this was added glucose (0.15%) and incubated 49 days to test for possible cometabolism of hydrocarbons. Hydrocarbons remaining in the growth …

Partial Characterization Of The Major Lipooligosaccharide From A Strain Of Haemophilus Ducreyi, The Causative Agent Of Chancroid, A Genital Ulcer Disease, William Melaugh, Nancy J. Phillips, A A. Campagnari, R Karalus, B W. Gibson

Chemistry Faculty Publications

The first preliminary structure of a surface lipooligosaccharide from Haemophilus ducreyi has been determined. The major oligosaccharide was released by mild acid hydrolysis and analyzed by liquid secondary ion and tandem mass spectrometry. The mass spectral data combined with composition and methylation analysis yielded the most probable structure; Gal1----4GlcNAc1----3Gal1----4Hep1----6Glc1----( Hep1----2Hep1----)3,4Hep1---- KDO, where the reducing terminal 3-deoxy-D-manno-octulosonic acid (or KDO) exists in an anhydro form. This anhydro species results from the elimination of a phosphate from C-4 of KDO during mild acid hydrolysis. The core heptose trisaccharide consists of L-glycero-D-manno-heptose, but analysis of the peracetylated sugars indicated that the 1,4-linked heptose …

How Arsenic Residues Get In Wool, Tony Martin, Robin Jacob, Marion Davies, Peter Rutherford

Journal of the Department of Agriculture, Western Australia, Series 4

Wool can become contaminated with arsenic in various ways, and several different sources may each contribute to any individual arsenic residue problem.

• dipping sheep in an arsenical dip ( now illegal),

• dipping sheep in a non-arsenical dip in a contaminated dipping facility

• penning sheep on soil with high levels of arsenic before shearing.

Other possible sources include running sheep on land contaminated by gold mine tailings or exploration sites, and allowing sheep access to sites on the farm where arsenical compounds have been dumped, for example, rubbish dumps or sites where dip/sump sludge has been dumped.

Farmers …

A Proposal For United States/Polish Collaboration On Energy And The Environment, Carole B. Szpunar

Chemistry: Faculty Publications and Other Works

Argonne National Laboratory, a U.S. Department of Energy facility that is operated by the University of Chicago and located southwest of Chicago, Illinois, has proposed a collaborative effort by the United States and Poland to conduct applied research on energy and environmental topics of common interest. Through this collaboration, evaluative studies on energy and environmental assessment issues could be developed and implemented by Argonne and one or more sister institutions in Poland, which could lead to active research, development, and demonstration joint activities. The objective of this initiative is to produce strategies and options for review by Poland's decision makers …

Improved Recovery Of A Radlolabeled Peptide With An Albumin-Treated Reversed-Phase Hplc Column, David S. Hage, Robert L. Taylor, Pai C. Kao

David Hage Publications

Reversed-phase high-performance liquid chromatography (RP-HPLC) is an important tool in the purification of radiolabeled peptides and proteins for immunoassay. However, for some proteins and peptides it is difficult to achieve reproducible behavior in RP-HPLC because of the low recovery of these compounds. Factors that can be varied to improve recovery include the strength or pH of the mobile phase, the chain length and spacing of groups on the reversed-phase support, and the flow rate or steepness of the elution gradient (1-5). ... In summary, we obtained better recovery and more reproducible chromatographic behavior for labeled 1-34 PTHrP with an albumin-pretreated …

Intact Parathyroid Hormone: Performance And Clinical Utility Of An Automated Assay Based On High-Performance Immunoaffinity Chromatography And Chemiluminescence Detection, David S. Hage, Bob Taylor, Pai C. Kao

David Hage Publications

The performance and clinical utility of an automated assay of intact parathyroid hormone (parathyrin, PTH) are evaluated. The method is based on the extraction of PTH from plasma by an HPLC column containing immobilized anti-(44-68 PTH) antibodies. The PTH retained is detected with a postcolumn reactor and use of anti-(1--34 PTH) chemiluminescent-labeled antibodies. The total cycle time of the assay is 6.5 mm per injection after a 1-h incubation.The lower limit of detection for PTH in a 66-pL plasma sample was 0.5 pmol/L based on peak heights and 0.2 pmol/L based on peak areas. Mean analytical recovery for PTH added …

Consumption Rates, Evacuation Rates And Diets Of Pygmy Killifish, Leptolucania Ommata, And Mosquitofish, Gambusia Affinis In The Okefenokee Swamp, J. Douglas Oliver

Faculty Publications and Presentations

I studied feeding dynamics of Leptolucania ommata and Gambusia affinis in the Okefenokee Swamp. Both fishes mainly ate insect larvae (such as Chironomidae) and Cladocera. Evacuation rates ranged from 0.143 (L. ommala in winter) to 0.279/hour (L. ommala in summer). Daily food consumption (dry weight) ranged from 24.2 (L. ommata in winter) to 148.3 mg/g/day (G. affinis in summer). Maximum consumption by both species was estimated at 26.31 mg/t m2 / day, in summer. These values are consistent with other observations supporting a hypothesis that invertebrate prey production is substantial in these blackwater wetlands.

Bulletin No 4222 - Management Of Properties With Pesticide Residues - Information For Farmers With Properties Affected By Organochlorine Pesticide Residues, Farran Dixon

Bulletins 4000 -

This publication is produced by the Department of Agriculture to help farmers who have been affected by the pesticide residue problem. Some material from previous issues has been included, so that as much information as possible is contained in a single publication. We suggest you keep it as a convenient reference on any aspect of the residue problem.

All owners of affected land are urged to read this publication carefully. This applies to the owners of any land which has a soil organochlorine residue level greater than 0.1 parts per million (ppm).

A management notice will be issued for each …

Expression Of Biologically Active Human Corticosteroid Binding Globulin By Insect Cells: Acquisition Of Function Requires Glycosylation And Transport, Jayasri Ghose-Dastidar, J. B. Alexander Ross, Reza Green

Chemistry and Biochemistry Faculty Publications

Human corticosteroid binding globulin (hCBG) is a 50- to 55-kDa serum glycoprotein that binds cortisol and progesterone with high affinity. To map the steroid-binding domain and to investigate the folding pathways of hCBG, we have established an expression system based on infection of insect cells with a recombinant baculovirus encoding hCBG. Infected Spodoptera frugiperda (Sf9) cells secrete immunoreactive hCBG at high levels (16-24 pmol per 10⁶ cells per 40 h), and the recombinant protein binds cortisol with an affinity and specificity equivalent to that of human serum-derived hCBG. Thus, this system has the potential to provide large amounts of …

Ua3/8/1 John Robinson Professorship Report, Wku Ogden College Of Science & Technology

WKU Administration Documents

Report by Dr. John Riley, John Robinson Professor of activities for 1990-1991 funded by Ogden Foundation.

Bulletin No 4219 - Chemical Control Of Insect Pests In Field Crops And Pastures, T. P. Quinn, G. P. Mangano, P. J. Michael

Bulletins 4000 -

Chemicals currently registered in Western Australia for use against pests of field crops and pasture are given in the following tables. Trade names are listed alongside each chemical formulation.

Department of Agriculture Bulletin 4185 "Insect and allied pests of extensive farming" will help you identify the pest(s) through coloured photographs, descriptions and keys. Information on the type of damage, life cycle and control is also provided. Further information is available in Farmnotes on specific pests and on pests of a particular crop.

The Poison Schedule for each product is indicated in the table. Extremely toxic chemicals are labelled as "dangerous …

Use Of Affinity Chromatography In Developing Acridinium Ester-Labeled Antibodies For An Immunometric Assay Of Parathyrin, David S. Hage, Bob Taylor, Pat Schryver, Pai C. Kao

David Hage Publications

In developing an immunometric assay of intact parathyrin (parathyroid hormone, PTH), we found that affinity chromatography is a useful tool in purifying and optimizing the labeling conditions for acridinium ester-labeled antibodies. ... In summary, affinity chromatography was found to be useful in the purification of acridinium ester-labeled antibodies, particularly for removing denatured antibodies from the prepared label and for monitoring the amount of active labeled antibodies produced.

The Effect Of Nitrogen On Pastures And Subsequent Sheep Production., L. Cransberg

Experimental Summaries - Plant Research

Trial 89MT1

Location: Mt Barker Research Station

To measure the effect of autumn/winter application on:

1. Pasture growth rates and composition

2. Sheep production using pregnant/lacting ewes.

Trial 89KA6

Management systems for sheep.

Location: Kojonup

To measure the effects of set stocking and strip (rotation) grazing on:

1. Pasture production, composition and quality.

2. Sheep production (liveweight, condition score, wool growth rate, fibre diameter strength and vegetable matter contamination).

Seed Phosphorus In Wheat And Lupins, Blakely Paynter, K. Adcock, R. W. Bell, Tim Pope

Experimental Summaries - Plant Research

Trial 90NO119

Seed P in wheat.

Location: Greenhills.

Design: 2 applied P x 3 seed P x 4 replicates, sowing wheat (cv. Aroona) seed from 89N33 at 70 kg/ha on May 15, 1990.

Trial 90NO120

Seed P in wheat.

Location: York

Design: 2 applied from P x 3 seed P x 4 replicates, 89N33 at 70 kg/ha on May 25, 1990.

Trial 90NO117

Seed P in lupins

Location: Greenhills.

Design: 2 applied P x 3 seed P x 4 replicates, sowing lupins (cv. Gungurru) from 85BA35 at 100 kg/ha on May 15, 1990.

Trial 90NO118

Seed P in lupins.

Location: …

Development Of Procedures To Determine The Fertilizer Requirements Of Annual Pasture Legumes Grown In Cereal Cropping Systems., Blakely H. Paynter

Experimental Summaries - Plant Research

Trials 88ME67, 88SC17, 90ME1 and 90ME2

Location: Merredin

To determine the phosphate (P) fertilizer requirements of burr medic on marginally acidic, medium textured soils and yellow serradella on acidic, light textured soils.

Trials 89ME52, 89ME55 and 86ME61

Location: Merredin

Determine the response of yellow serradella and burr medic to residual phosphate and freshly applied phosphate.

Trials 89ME50, 89ME54, 88ME67, 88SC17, 89ME60

Location: Merredin.

To measure the influence of different levels of pasture productivity (generated by different levels of P supply) and the different pasture legumes on subsequent wheat crops.

Drop-Box Technique For Quantitative Sampling Of Macrofauna And Aquatic Plants At Restored And Natural Wetlands, J. Douglas Oliver, Andrew J. Leslie, William M. Bartodziej

Faculty Publications and Presentations

No abstract provided.

Activator-Inhibitor Control Of Tissue Growth, John A. Adam

Mathematics & Statistics Faculty Publications

This note develops a simple model for the competition between activator and inhibitor control mechanisms in one-dimensional tissue growth. The pedagogic usefulness of such a model is that it is easily accessible to undergraduate applied mathematicians and is suggestive of behavior known to occur in more realistic biological systems (e.g., some types of cancer). The limitations of the model are obvious and can provide a basis for discussion of the applicability of complementary levels of description in mathematical modeling.

The Role Of The Voltage Gradient In The Agarose Gel Electrophoresis Of Dna, David Wheeler

Theses and Dissertations in Biomedical Sciences

In Part I of this dissertation, empirical equations for predicting DNA mobility during agarose gel electrophoresis (AGE) from voltage gradient are developed from the data of McDonnel (36) for electrophoresis in a 1.6% agarose gel. These equations represented the data well for DNA between 2 and 10 kilobase pairs (KBp) in length. A computer program, called GELSIM, which incorporates these equations is described in Part II. GELSIM was designed to allow researchers to analyze electrophoresis data by predicting the effect on DNA migration of altering the voltage of electrophoresis. In this way, electrophoretic banding patterns produced using different voltages could …

Pea And Faba Bean Symbiosis With Rhizobium., J. Evans, C. Wallace, N. Dobrowolski

Experimental Summaries - Plant Research

Trial 89WH55, 89KA57, 89M26, 90A12, 90A13, 90M59, 90M60

Location: Merredin, Konnongorring, Dalwallinu, Wongan Hills, Kojonup, Avondale Research Station.

To undertake relevant field studies to establish whether there are constraints to pea and bean symbiosis, arising from management, environment or genetics, and affecting crop N accumulation and productivity.

Trial 90BA60

Using Le-mat (omethoate) insecticide and legume inoculant on seed.

Location: Badgingarra Research Station,

To measure the effect of Lemat on the nodulation and growth of medics and luceme.

Rhizoctonia Bare Patch Of Cereals And Lupins. Sterile Red Fungus (Srf) And Control Of Take-All Disease In Wheat., G. C. Macnish

Experimental Summaries - Plant Research

Trial 86E31

Rhizoctonia strains and paddock history.

Location: Esperance Downs Research Station ( Paddock C5)

To determine the effects of pasture, cultivation and cropping on Rhizoctonia root rot, Rhizoctonia patch and distribution of patch and non-patch strains of R. spp

Trial 88E39

Rhizoctonia patch mini-plot establishment area.

Location: Esperance Downs Research Station ( Paddock N1B)

To examine the effects of cultivation and minimum tillage in patches in lupins and to provide sites for mini-plot experiment.

Trial 88KO15

Rhizoctonia bare patch establishment area.

Location: Kojaneerup annex.

To establish an area (with a known history) for future research.

Trial 89E12

Rhizoctonia bare …