Veterinary Medicine Commons^™

The Effect Of Late Gestation Supplementation Strategy On Cow-Calf Performance In March-Calving Mature Cows, J. A. Musgrave, Kacie L. Mccathy, J. Travis Mulliniks

Nebraska Beef Cattle Reports

Implementation of supplemental protein strategies during late gestation has been shown to positively affect postweaning progeny performance. A 2-yr study was conducted to evaluate the effects of late gestation supplementation strategies on reproduction, cow body weight, and calf performance in March-calving mature range cows grazing dormant upland range. Supplementation was individually fed and provided daily with treatments being: 1) no supplementation, 2) 2 lb per day of a 30% CP distillers-based supplement, 3) 2 lb per day of a 30% CP distillers-based supplement with the inclusion of 160 mg/cow daily of monensin, 4) 2 lb per day of a 30% …

Effect Of Glucogenic Feed Additive On Reproductive Performance In Young Postpartum Range Cows, Tasha M. King, J. A. Musgrave, Nicole M. Woita, Shelby L. Boerman, J. C. Macdonald, J. Travis Mulliniks

Nebraska Beef Cattle Reports

Performance of young March-Calving range cows receiving a protein supplement with the addition of either monensin or propionate salt were compared to evaluate the effect of feed additive on overall production in the postpartum stage. cow body weight and body condition were not impacted by postpartum supplementation throughout the study. Calf body weights were not impacted by type of feed additive at birth, weaning, or 205-d. Twenty-four-hour milk production was not impacted by the type of feed additive. Conception rates for cows receiving postpartum supplementation containing propionate salt were greater than cows receiving monsensin. This implies that the addition of …

Effect Of Heifer Percent Mature Body Weight At Breeding On Heifer Performance, Calf Production, And Subsequent Pregnancy Rates, Josie N. Crouch, J. Travis Mulliniks, J. A. Musgrave, Kathy J. Hanford, Kacie L. Mccathy

Nebraska Beef Cattle Reports

A retrospective study was done utilizing 1,434 March- and May- calving Red Angus x Simmental crossbred cows and heifers from 2005 through 2019. Heifer weight as a percentage of mature body weight at breeding was used to conduct a regression analysis from 50 to 70% of mature body weight to determine the impact of body weight at breeding on reproductive performance. Heifer pregnancy rates and second pregnancy rates as 2-year-olds were greater for heifers at 60, 65, and 70% of mature body weight at breeding than heifers at 50 and 55%. However, heifer percent mature body weight at breeding did …

Impact Of Increasing Level Of Milk Production On Cow-Calf Performance In Nebraska Sandhills, Selby L. Boerman, Jacki A. Musgrave, Kathryn J. Hanford, Mitchell B. Stephenson, J. Travis Mulliniks

Nebraska Beef Cattle Reports

In a 2-yr study, data were collected on 118 crossbred cow-calf pairs from March and May-calving herds. On approximately 30, 60, 90, 120, and 210 d postpartum, individual cow 24-h bilk yield was estimated through weigh-suckle weigh techniques. Cow body weight (BW) and body condition score (BCS) were collected weekly through breeding. Calf BW was recorded at each milking. Individual cow milk area under the curve (AUC) values were calculated and data were analyzed using linear regression analysis. Results from this study illustrate that increasing total milk produced throughout the lactation period had minimal influence on the cow production parameters …

Landscape-Scale Epidemiological Dynamics Of Sars-Cov-2 In White-Tailed Deer, Joshua Hewitt, Grete Wilson-Henjum, Derek T. Collins, Timothy J. Linder, Julianna B. Lenoch, Jonathan D. Heale, Christopher A. Quintanal, Robert Pleszewski, Dillon S. Mcbride, Andrew S. Bowman, Jeffrey C. Chandler, Susan A. Shriner, Sarah N. Bevins, Dennis J. Kohler, Richard B. Chipman, Allen L. Gosser, David L. Bergman, Thomas J. Deliberto, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding pathogen emergence in new host species is fundamental for developing prevention and response plans for human and animal health. We leveraged a large-scale surveillance dataset coordinated by United States Department of Agriculture, Animal and Plant Health Inspection Service and State Natural Resources Agencies to quantify the outbreak dynamics of SARS-CoV-2 in North American white-tailed deer (Odocoileus virginianus; WTD) throughout its range in the United States. Local epidemics in WTD were well approximated by a single-outbreak peak followed by fade out. Outbreaks peaked early in the northeast and mid- Atlantic. Local effective reproduction ratios of SARS-CoV-2 were between …

What Is Known, Unknown, And Needed To Be Known About Damage Caused By Wild Pigs, Kurt C. Vercauteren, Kim M. Pepin, Seth M. Cook, Sophie Mckee, Abigail Pagels, Keely J. Kohen, Ingrid A. Messner, Michael P. Glow, Nathan P. Snow

United States Department of Agriculture Wildlife Services: Staff Publications

Damage assessments provide evidence for initiating and evaluating management programs that protect natural resources and human livelihoods against invasive species. Wild pigs (Sus scrofa) cause extensive damage in their native and non-native ranges, but the extent of current damage and efficacy of management of the damage (i.e., population control of wild pigs, exclusion fencing, etc.) remains poorly described. We conducted a systematic review of physical damage caused by wild pigs to summarize what is known and identify knowledge gaps for damage assessment. Wild pig damage assessments have been overwhelmingly qualitative (84% of studies) and measured differently across studies, …

Tracking Sero-Molecular Trends Of Swine Brucellosis In Hawai'i And The Central Pacific, Thi Hai Au La, Ian A. Mcmillan, Prashant Dahal, Andrew H. Burger, Mahdi Belcaid, Darrin M. Phelps, Samuel M. Goldstein, Vienna R. Brown, Michael H. Norris

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction

Brucellosis is a zoonotic disease of mammals caused by bacterial species of the Brucella genus. The reservoir for disease is typically mammals, with species of Brucella found infecting amphibians, bats, and marine mammals. Brucella spp. can pass directly to humans through contact with infected animals or their products. Brucella spp. can cause chronic debilitating infections in mammals, including humans, and is associated with spontaneous abortions in infected animals, causing reduced fecundity. In Hawai‘i, terrestrial species that could harbor Brucella spp. include swine, cattle, horses, and axis deer among others. The numerous feral swine in Hawai‘i are known to carry …

Spatial Analysis Of Socioeconomic Factors At Found Locations Of Orphaned Mammals In Champaign County, Illinois, Usa, Colleen Elzinga, Stephanie Heniff, William Sander, William Marshall Brown, Csaba Varga, Samantha Sander

Human–Wildlife Interactions

Young mammals make up a significant portion of animals brought into wildlife rehabilitation centers. Unfortunately, some of these presumed orphaned individuals are inappropriately removed from their native habitat. As such, there is a need for educational community outreach centered on the proper identification of orphaned wildlife that benefit from human intervention, while maximizing limited resources of rehabilitation facilities. This study evaluated found locations of common mammalian orphans presenting from Champaign County to the University of Illinois Wildlife Medical Clinic, Illinois, USA, between 2015 and 2020. The found locations of the orphans (99 Virginia opossums [Didelphis virginiana], 80 common …

Genetic Tracking Of A Rabid Coyote (Canis Latrans) Detected Beyond A Rabies Enzootic Area In West Virginia, Usa, Matthew W. Hopken, Crystal Gigante, Amy T. Gilbert, Richard B. Chipman, Jordana D. Kirby, Rene Edgar Condori, Samuel Mills, Chelsea Hartley, John Forbes, Lisa Dettinger, Dongxiang Xia, Yu Li, Bridgett Vonholdt

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife translocation and cross-species transmission can impede control and elimination of emerging zoonotic diseases. Tracking the geographic origin of both host and virus (i.e., translocation versus local infection) may help determine the most effective response when high-risk cases of emerging pathogens are identified in wildlife. In May 2022, a coyote (Canis latrans) infected with the raccoon (Procyon lotor) rabies virus variant (RRV) was collected in Lewis County, West Virginia, USA, an area free from RRV. We applied host population genomics and RRV phylogenetic analyses to determine the most likely geographic origin of the rabid coyote. Coyote …

Measurement Of 8-Hydroxy-2′-Deoxyguanosine In Serum And Cerebrospinal Fluid Of Horses With Neuroaxonal Degeneration And Other Causes Of Proprioceptive Ataxia, Megan Palmisano, Jeaneen Kulp, Susan Bender, Darko Stefanovski, Mary Robinson, Amy Johnson

Faculty, Staff and Student Publications

BACKGROUND: Eight-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative damage evaluated in human neurodegenerative disease, has potential to correlate with postmortem diagnosis of neuroaxonal dystrophy/degenerative myeloencephalopathy (NAD/DM) in horses.

HYPOTHESIS: We hypothesized that 8-OHdG will be higher in CSF and serum from NAD/DM horses compared with horses with other neurologic diseases (CVSM, EPM) and a control group of neurologically normal horses. We also hypothesized that 8-OHdG will be higher in CSF compared with serum from NAD/DM horses.

ANIMALS: Fifty client-owned horses with postmortem diagnoses: 20 NAD/DM, 10 CVSM, 10 EPM, and 10 control horses. Serum and CSF samples were obtained between November …

Distribution Pattern And Risk Factors Of Foot And Mouth Disease Outbreaks In Java Island, Indonesia, Virgilius Martin Kelake Kedang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Indonesia experienced new outbreaks of foot and mouth disease (FMD) in 2022 after being officially free from the disease for several decades. The outbreak started in April 2022 in East Java and rapidly spread to many regions in Indonesia. This study aimed to investigate the distribution pattern and risk factors associated with the FMD outbreaks in Java Island, Indonesia, from 2022 to 2023. Descriptive, spatial, spatiotemporal, and risk factor analyses were employed to examine the outbreak dynamics and contributing factors. The results indicated that the outbreaks were distributed across the island, with East Java being the most affected region. The …

Epidemiological And Molecular Studies On Lumpy Skin Disease Outbreaks In Beef Cattle In Thailand During 2021-2022, Phurida Sripipattanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lumpy skin disease (LSD) is a vector-borne viral disease of cattle caused by the Lumpy skin disease virus (LSDV). LSD is considered as an emerging infectious disease in cattle in Asia and poses a significant threat to cattle production across Africa, Europe, and Asia. This study aimed to describe the epidemiological characteristics of LSD outbreaks in beef cattle in Thailand and to conduct a molecular survey of LSDV infection in beef cattle from May 2021 to December 2022. The study included 594 beef cattle from 257 farms affected by the LSD outbreaks in 28 provinces. We collected hair samples (n=492) …

Isolation, Molecular Characterization, And Epidemiological Analysis Of An Emerging Avian Metapneumovirus Subgroup B In Us Poultry, Sumit Jangra

Electronic Theses and Dissertations

Avian metapneumovirus (aMPV) is one the most economically important poultry respiratory pathogens belonging to the family Pneumoviridae and genus Metapneumovirus, which can affect both domestic and wild birds. aMPVs are classified into four subgroups (A, B, C, and D) based on the level of genetic variations and antigenic differences. aMPV causes turkey rhinotracheitis (TRT) and swollen head syndrome (SHS) in turkeys and chickens respectively. Recently, outbreaks of respiratory disease in meat-type birds and a significant decline in egg production with respiratory signs in breeders were reported from different states in the USA. Isolating aMPV is challenging as it is a …

Comparison Of Nonlinear Models For Predicting Live Weight Growth Curves In Lambproduction Of Kıvırcık And Karacabey Merino, Vasfi̇ye Kader Esen, Orhan Karadağ, Cengi̇z Elmaci, Seli̇m Esen

Turkish Journal of Veterinary & Animal Sciences

To achieve sustainable lamb production, it is crucial to determine the optimal slaughter age for maximum economic efficiencyand have a comprehensive understanding of breed characteristics. This study focuses on evaluating different nonlinear models todetermine the optimal slaughter age for Karacabey Merino and Kıvırcık lambs, renowned for their early development and superiormeat quality, respectively. The dataset comprised age-related live weight data from 98 lambs, including 36 female and 15 male Kıvırcıklambs, as well as 33 female and 14 male Karacabey Merino lambs. Six models, namely, negative exponential, Brody, Gompertz, logistic,Bertalanffy, and Richards were utilized to analyze the data. The model parameters …

Measurement Of 8-Hydroxy-2′-Deoxyguanosine In Serum And Cerebrospinal Fluid Of Horses With Neuroaxonal Degeneration And Other Causes Of Proprioceptive Ataxia, Megan Palmisano, Jeaneen Kulp, Susan Bender, Darko Stefanovski, Mary Robinson, Amy Johnson

Faculty, Staff and Student Publications

BACKGROUND: Eight-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative damage evaluated in human neurodegenerative disease, has potential to correlate with postmortem diagnosis of neuroaxonal dystrophy/degenerative myeloencephalopathy (NAD/DM) in horses.

HYPOTHESIS: We hypothesized that 8-OHdG will be higher in CSF and serum from NAD/DM horses compared with horses with other neurologic diseases (CVSM, EPM) and a control group of neurologically normal horses. We also hypothesized that 8-OHdG will be higher in CSF compared with serum from NAD/DM horses.

ANIMALS: Fifty client-owned horses with postmortem diagnoses: 20 NAD/DM, 10 CVSM, 10 EPM, and 10 control horses. Serum and CSF samples were obtained between November …

Evaluating The Efficacy Of An Early Vaccination Strategy After Farrowing Using A Modified Live Prrs Vaccine And Post-Weaning Challenge With Thai Hp-Prrs, Apichart Prapattanakullawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen responsible for substantial economic losses in the global swine industry, adversely affecting both reproductive and respiratory health. In Thailand, early PRRSV infections in suckling and nursery pigs are commonly observed, necessitating the implementation of early vaccination strategies. However, early vaccination in young piglets has been controversial due to concerns about interfering maternal-derived antibodies (MDA) and the competency of the piglets' immune system. The objective of this study is to evaluate the efficacy of a modified-live virus (MLV) PRRS vaccination in early-age piglets from PRRSV-positive farms by assessing immune responses …

Genotypic Characterization And Amr Risk Assessment Of Esbl- Producing And Fluoroquinolone-Resistant Salmonella Spp. From Pork Collected In Bangkok, Thawanrut Kiatyingangsulee

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to 1) determine the prevalence of ESBL-producing and fluoroquinolone-resistant Salmonella in pork, 2) investigate their genomic characteristics, and 3) estimate the risk of consumer exposure. Pork samples were collected from 58 markets across 19 districts in Bangkok. The study focused on third-generation cephalosporin resistance mediated by ESBLs and fluoroquinolone resistance, representing horizontal and vertical gene transfer, respectively. Out of 793 pork samples, 339 (42.8%) were positive for Salmonella, yielding 531 isolates from 33 serotypes. The most common were Salmonella Rissen (22.6%), Sinstorf (16.8%), and Derby (10.2%). Antimicrobial susceptibility testing showed 28.2% of isolates were ESBL producers and …

Efficacy Of Cell-Free Supernatants Based Nano-Application From Thai Probiotic Bacteria As An Alternative Preservative In Meat Against Foodborne Pathogens, Thin Zar Soe

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study evaluates whether the use of cell-free supernatants (CFS) derived from Thai probiotic bacteria as natural preservatives in meat products, provides a safer alternative to chemical preservatives. The objectives include developing a nano-encapsulation technique to enhance the stability and efficacy of CFS, and assessing the effectiveness of nano-encapsulated CFS (Nano-CFS) in maintaining meat safety and quality during storage and evaluating the antibacterial efficacy of CFS and (Nano-CFS) against common foodborne pathogens, determining the minimal inhibitory and bactericidal concentrations (MIC and MBC). The CFS was prepared from the Thai probiotic strains. Antibacterial activity was determined using agar well diffusion and …

Genomics Approach For Ex Situ Conservation Of Asian King Vulture And Steppe Eagle, Wannapol Buthasane

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Asian king vulture (Sarcogyps calvus, AKV), an ecologically important forest scavenger, is an Old World vulture in the family Accipitridae. It is classified as a critically endangered species, with no genomic data currently available. This dissertation aimed to assemble a high-quality reference genome to enhance understanding of its genetic background, scavenging adaptations and genetic diversity within Thailand’s captive population. A hybrid genome assembly combining Pacific Biosciences (PacBio) long-read and MGI short-read sequencing was generated from DNA extracted from the whole blood of a female individual. Comparative genomic analyses with New World vultures (NWVs) and other Accipitridae species revealed distinct …

The Comprehensive Study Of The Reproductive Cycle With A Special Focus On The Vestibular Microbiome In Captive Asian Elephants (Elephas Maximus), Yuqing Yang

Chulalongkorn University Theses and Dissertations (Chula ETD)

This dissertation aimed to advance the understanding of reproductive physiology and health in Asian elephants, providing critical insights and innovative methodologies for reproductive management. Chapter II established a foundational framework for normal reproductive cycling, detailing the luteal and follicular phases, and introduced a simplified, practical body condition score (BCS) method as a diagnostic tool for assessing general health and welfare. It highlighted unresolved reproductive issues, such as abnormal cycling, and revealed that progesterone peaks, with the majority (53.57%), and the onset of the follicular phase predominantly occurred during the rainy season. Monitoring ovulation and maintaining a standard BCS during the …

Improving Antimicrobial Susceptibility Testing Of Arcobacter Butzleri: Comparative Methods And Epidemiological Cut-Off Value (Ecoff) Determination, Myzatul Hanis Zahiyah Yusof

Chulalongkorn University Theses and Dissertations (Chula ETD)

Arcobacter butzleri is an emerging zoonotic pathogen associated with gastrointestinal illnesses in humans, yet standardized antimicrobial susceptibility testing (AST) methods and interpretive breakpoints for this microorganism remain unavailable. Broth microdilution, despite being endorsed by the CLSI-VAST subcommittee, is often impractical in several laboratories. Therefore, this study aimed to evaluate agar dilution as a more feasible alternative to broth microdilution. A total of 415 A. butzleri isolates from various meat sources in Bangkok, Thailand were confirmed by PCR and tested against ciprofloxacin, erythromycin, gentamicin, and tetracycline. AST was conducted using broth microdilution under aerobic conditions for 24 hours as the reference …

Ultrasonographic Renal Cortical Thickness To Abdominal Aorta Internal Diameterin Healthy Cats And Chronic Kidney Disease Cats, Run Sakulsirajit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chronic kidney disease (CKD) is a one of the most common disorder in cats, particularly in aging populations. Early detection and accurate monitoring are crucial for effective disease management. This study aims, first, to evaluate the ultrasonographic parameters, including renal cortical thickness (RCT), medullary thickness (MT), renal length (RL), abdominal aorta diameter (Ao) and ratios, including RCT/Ao, RCT/MT, RCT/RL, MT/Ao and RL/Ao compared between clinically healthy and CKD cats. Second, to evaluate the correlations of ultrasonographic parameters with blood renal functions including blood urea nitrogen (BUN), creatinine (Cr) and symmetric dimethylarginine (SDMA) and also correlation with age, body weight (BW) …

Complete Genome Analysis Of African Swine Fever Virus Strains In Thailand, Anwesha Prakash

Chulalongkorn University Theses and Dissertations (Chula ETD)

African Swine Fever Virus (ASFV) is a large double-stranded DNA virus responsible for the highly contagious and lethal African Swine Fever (ASF), affecting the global porcine industry. The virus has a genome length of 170-194 kb and is broadly categorized into the left variable region (LVR), the right variable region (RVR), and the central conserved region (CCR). In Thailand, ASF was officially confirmed in January 2022 and identified as genotype II, which is primarily responsible for the current Eurasian swine pandemic. To date, due to the absence of a globally accepted vaccine against ASFV, culling and quarantine remain the primary …

Canine Cricoid Cartilage And Tracheal Dimensions Observed On Computed Tomographic Image, Tanya Kasemsuwan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Inhalation anesthesia is commonly used for general anesthesia due to its effectiveness. During the procedure, an endotracheal tube (ET) is essential for maintaining anesthetic depth, delivering anesthetic agents, and providing sufficient oxygen to reduce the risks of morbidity and mortality. Mispositioning and inappropriate ET size can lead to complications like hypoxemia, hypercapnia, and airway trauma. Commercially available ET tubes are designed for general use and are not specifically optimized for different cephalic types, leading to the objective of this study, which was to investigate the differences in anatomical dimensions of the cricoid cartilage and trachea and ET location among brachycephalic, …

Effect Of Four Non-Slipping Floor Mat Types On The Stifle Joint Range Of Motion During Walking And Trotting In Small Breed Dogs With Medial Patellar Luxation Grade 2 And Normal Stifle Dogs, Panisara Wongsirichatchai

Chulalongkorn University Theses and Dissertations (Chula ETD)

The two dimension kinematic motion analysis was an accessible tool for clinical approach for assist gait analysis and joint mobility in veterinary clinical field. Thermographic imaging also considered as an adjunctive non-invasive technique to detects changed superficial temperature from the physiological associated with inflammation or musculoskeletal conditions especially in joints, tendon, or muscle. A comparing controlled experiment investigated the effects of various ceramic flooring surfaces on gait, stifle joint mobility, and post-activity inflammatory response following activity in small breed dogs with and without grade 2 medial patellar luxation (MPL).Sixteen small breed dogs were enrolled, including normal stifle dogs (n=7) and …

Validation Of A Pseudotype-Based Serum Neutralization Test Forpeste Des Petits Ruminants Virus (Pprv) Specific Antibody In Goat Sera, Suphatcha Mahawattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Peste des petits ruminants (PPR) is a transboundary viral disease in small ruminants, caused by a morbillivirus called peste des petits ruminants virus (PPRV). Recently, FAO and WOAH have set the goal to eradicate the disease in 2030. To maintain disease vigilance, the Department of Livestock Development conducts regular screenings for anti-PPRV antibodies using a commercially available competitive ELISA (cELISA). However, limitation from its window of uncertainty could lead to inconclusive result. In this study, a recently developed pseudotype-based neutralization test was validated against a large number of known positive and negative samples. In comparison with the cELISA, from 416 …

Proteomic Analysis Of Canine Reproductive Protein Profiles In Relation To Stage Of Oocyte Development, Larindhorn Udomthanaisit

Chulalongkorn University Theses and Dissertations (Chula ETD)

The domestic dog has a unique reproductive physiology in which oocytes are ovulated at an immature stage and complete nuclear maturation within the oviduct approximately 48–72 hours after ovulation. This thesis aimed to investigate proteomic changes in both serum and oviductal secretions associated with oocyte development, using a combination of natural and hormonally induced ovulation models. To evaluate dynamic changes in serum proteins across the estrous cycle, samples from healthy bitches in four stages—anestrus, proestrus, estrus, and diestrus—were analyzed, revealing 38 significantly altered proteins. Among these, eight exhibited stage-specific expression patterns, including HES2, ADGRA3, and SWI5 in anestrus; polycystin receptors …

Alternative Approaches To Replace Antibiotics Use In Boar Semen Extenders And Their Effects On Reproductive Performance, Congbang Ngo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Artificial insemination (AI) with antibiotic-free alternatives in boar semen extenders is becoming essential for promoting environmentally sustainable practices in the global swine industry. Comprising 4 parts, this thesis aimed to investigate the boar fecal and seminal microbiomes in relation to semen quality and probiotic supplementation in an antibiotic-free feeding program. In addition, the characteristics of semen doses treated with alternative methods and their effects on the reproductive performance of sows inseminated with these semen doses are examined. (i) To investigate the fecal and seminal microbiomes of boars in relation to semen quality and the effects of transitioning from antibiotics to …

Molecular Investigation And Genetic Characterization Of Hemotropic Mycoplasma Spp. And Potential Role Of Flea Vectors In Goats In Thailand, Muhammad Shair Zaman Ghauri

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to investigate the identification of flea species infesting goats and to study the molecular prevalence and genetic characterization of hemotropic mycoplasmas in goats and fleas. A total of 500 blood and 79 flea samples were collected from goat farms across seven provinces in Thailand between January 2017 and October 2023. Among the 500 goats examined, 6.6% were infested with fleas, which were morphologically identified and molecularly characterized using mitochondrial (cox-I, cox-II) and nuclear (ITS1, EF-1α) markers as Ctenocephalides orientis. Additionally, 14,315 bp fragment of the C. orientis mitochondrial genome was analyzed, revealing a close evolutionary relationship with …

Selection Of Lactic Acid Bacteria Probiotics From Thai-Chick And Their Efficacy In Restoring Gut Microbiome And Salmonella Reduction In Broiler Challenged With Salmonella Typhimurium, Benjamas Khurajog

Chulalongkorn University Theses and Dissertations (Chula ETD)

Antimicrobial resistance (AMR), food safety, and sustainable poultry farming are critical global concerns driving the exploration of probiotics as alternatives to antibiotics. This study focused on the isolation, characterization, and evaluation of lactic acid bacteria (LAB) strains and the performance of two probiotics—ProCU (a blend of locally isolated LAB strains) and TOP GUT (a commercial Clostridium butyricum product)—in mitigating Salmonella infection, improving growth performance, and modulating the gut microbiota and resistome in broiler chickens. From 91 LAB isolates identified in chicken feces across Thailand, five strains (Ligilactobacillus salivarius BF12 and Pediococcus acidilactici BF9, BF14, BYF20, and BYF26) exhibited strong tolerance …