Veterinary Medicine Commons^™

Investigation Into Cardiacmyhc-Α 334–352-Specific Tcr Transgenic Mice Reveals A Role For Cytotoxic Cd4 T Cells In The Development Of Cardiac Autoimmunity, Meghna Sur, Mahima T. Rasquinha, Kiruthiga Mone, Chandirasegaran Massilamany, Ninaad Lasrado, Channabasavaiah B. Gurumurthy, Raymond A. Sobel, Jay Reddy

Jay Reddy Publications

Myocarditis is one of the major causes of heart failure in children and young adults and can lead to dilated cardiomyopathy. Lymphocytic myocarditis could result from autoreactive CD4+ and CD8+ T cells, but defining antigen specificity in disease pathogenesis is challenging. To address this issue, we generated T cell receptor (TCR) transgenic (Tg) C57BL/6J mice specific to cardiac myosin heavy chain (Myhc)-α 334–352 and found that Myhc-α-specific TCRs were expressed in both CD4⁺ and CD8⁺ T cells. To investigate if the phenotype is more pronounced in a myocarditis-susceptible genetic background, we backcrossed with A/J mice. At the fourth …

Gene Editing Of Pigs To Control Influenza A Virus Infections, Taeyong Kwon, Bianca L. Artiaga, Chester D. Mcdowell, Kristin M. Whitworth, Kevin D. Wells, Randall S. Prather, Gustavo Delhon, Mark Cigan, Stephen N. White, Jamie Retallick, Natasha N. Gaudreault, Igor Morozov, Juergen A. Richt

School of Veterinary and Biomedical Sciences: Faculty Publications

Proteolytic activation of the hemagglutinin (HA) glycoprotein by host cellular proteases is pivotal for influenza A virus (IAV) infectivity. Highly pathogenic avian influenza viruses possess the multibasic cleavage site of the HA which is cleaved by ubiquitous proteases, such as furin; in contrast, the monobasic HA motif is recognized and activated by trypsin-like proteases, such as the transmembrane serine protease 2 (TMPRSS2). Here, we aimed to determine the effects of TMPRSS2 on the replication of pandemic H1N1 and H3N2 subtype IAVs in the natural host, the pig. The use of the CRISPR/Cas 9 system led to the establishment of homozygous …

Nuclear Phosphoproteome Reveals Prolyl Isomerase Pin1 As A Modulator Of Oncogene-Induced Senescence, Rodrigo Mohallem, Uma K. Aryal

Purdue University Libraries Open Access Publishing Fund

Mammalian cells possess intrinsic mechanisms to prevent tumorigenesis upon deleterious mutations, including oncogene-induced senescence (OIS). The molecular mechanisms underlying OIS are, however, complex and remain to be fully characterized. In this study, we analyzed the changes in the nuclear proteome and phosphoproteome of human lung fibroblast IMR90 cells during the progression of OIS induced by oncogenic RASG12V activation. We found that most of the differentially regulated phosphosites during OIS contained prolyl isomerase PIN1 target motifs, suggesting PIN1 is a key regulator of several promyelocytic leukemia nuclear body proteins, specifically regulating several proteins upon oncogenic Ras activation. We showed that PIN1 …

Naturally Derived Phenethyl Isothiocyanate Modulates Induction Of Oxidative Stress Via Its N-Acetylated Cysteine Conjugated Form In Malignant Melanoma, Sotiris Kyriakou, Nikoletta Demosthenous, Tom Amery, Kyle J. Stewart, Paul G. Winyard, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Phenethyl isothiocyanate (PEITC) is a secondary metabolic product yielded upon the hydrolysis of gluconasturtiin and it is highly accumulated in the flowers of watercress. The aim of the current study was to assess the role of a naturally derived PEITC-enriched extract in the induction of oxidative stress and to evaluate its anti-melanoma potency through the regulation of its metabolism with the concurrent production of the N-acetyl cysteine conjugated by-product. For this purpose, an in vitro melanoma model was utilized consisting of human primary (A375) cells as well as metastatic (COLO-679) malignant melanoma cells together with non-tumorigenic immortalized keratinocytes (HaCaT). …

Growing South Dakota (Winter 2024), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 Continuing a Leadership Journey
[Page] 5 CAFES Leadership Team Grows
[Page] 7 Award-Winning Faculty & Staff
[Page] 9 New Endowment Holders Recognized
[Page] 10 Inaugural Precision Livestock Field School Offered at SDSU Cottonwood Field Station
[Page] 11 SDSU Celebrates Professional Program in Veterinary Medicine Students [Page] 13 Clay Carlson, SDSU Meat Science Graduate Student Approaches School and Life with Winning Mindset
[Page] 15 SDSU Alumni has been selected as the Head Coach of the South Dakota State University Livestock Judging Team
[Page] 16 Livestock Units Promote Hands-On Learning & Premier Research
[Page] 20 SDSU leads NSF-backed fertilizer development …

A Potentially Cat-Astrophic Missed Diagnosis: Systemic Autoimmune Disease, Or Something More Sinister?, Cameron Smith, Do, Mph, Amy Dechet, Md

Poster Presentations

No abstract provided.

Limitations Of Invasive Snake Control Tools In The Context Of A New Invasion On An Island With Abundant Prey, Shane R. Siers, Melia G. Nafus, Jereid E. Calaor, Rachel M. Volsteadt, Matthew S. Grassi, Megan Volsteadt, Aaron F. Collins, Patrick D. Barnhart, Logan T. Huse, Amy A. Yackel Adams, Diane L. Vice

United States Department of Agriculture Wildlife Services: Staff Publications

In October 2020, a new population of invasive brown treesnakes (Boiga irregularis) was discovered on the 33-ha Cocos Island, 2.5 km off the south coast of Guam, United States. Cocos Island is a unique conservation resource, providing refuge for many lizards and birds, including endangered species, which were extirpated from mainland Guam by invasive predators including brown treesnakes. We sought to evaluate the usefulness of toxic baiting with acetaminophen-treated carrion baits and cage trapping, common tools for the control of brown treesnakes on mainland Guam, as potential eradication tools on Cocos Island. We evaluated multiple bait types and …

Site-Specific Space Use And Resource Selection By Black Vultures (Coragyps Atratus) In The Southeastern Usa, Betsy Evans, John S. Humphrey, Eric A. Tillman, Michael L. Avery, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

North American populations of Black Vultures Coragyps atratus have increased and expanded their distribution in the southern and eastern USA. In conjunction with these patterns has been a rise in human–vulture conflicts. To improve our understanding of space use patterns and better inform management, we evaluated the movements of Black Vultures (n = 23) in the southeastern USA using a long-term GPS tracking database. Our specific objectives were to: (1) quantify home-range sizes in relation to season and geographical study location and (2) examine within-home-range resource selection to identify landscape and anthropogenic factors influencing roost and diurnal space use. …

Antipredator Blood-Squirting Defense In Horned Lizards (Phrynosoma): Chemical Isolation Of Plasma Component(S), Pogonomyrmex Ant Dietary Origin, And Evolution, Wade C. Sherbrooke, Bruce A. Kimball

United States Department of Agriculture Wildlife Services: Staff Publications

North American horned lizards of the genus Phrynosoma are largely myrmecophagous and squirt systemic blood from circumocular sinuses during encounters with certain mammalian predators. The predators react with distinct revulsion. Using bioassay-guided fractionation of blood plasma, we endeavored to identify the source and characteristics of the active chemical compound(s) responsible for this negative oral response. Our results of coyote bioassays and a series of novel mouse bioassay experiments were largely concordant. The active compound(s) was likely plasma-borne with a molecular weight (mw) of between 800 and 1,600. To identify the source of the active compound(s), we noted bioassay responses …

Eradication Of Feral Swine From A Barrier Island In Florida, Usa: An Examination Of Effort And Multi-Method, Multi-Species Population Indexing, Richard M. Engeman, Eric A. Tillman, Betsy A. Evans, John C. Griffin, Garrison Grobaski, Bradley S. Smith, John Stark, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine (Sus scrofa) were targeted for and successfully eradicated from Saint Vincent Island (SVI), a National Wildlife Refuge (NWR) along the coast of Florida’s panhandle to protect its habitats and uncharacteristically high diversity of wildlife species for barrier islands in the region, including federal and state-listed threatened and endangered species. The eradication effort was initiated in early 2015 and concluded in 2019. A total of 438 feral swine were removed from the Island, 417 by federal control experts and 21 by recreational hunters. In general, the amount of effort needed to eradicate each feral swine slowly increased …

Downed Wood Removal Effects On Survival And Site Fidelity Of Woodrats In A California Oak Woodland, Timothy J. Smyser, Michael A. Hardy, Amy J. Davis, William L. Preston, William D. Tietje

United States Department of Agriculture Wildlife Services: Staff Publications

Owing to the increasing intensity and frequency of wildfires in the western United States, the removal of woody debris (downed dead wood and snags) from fire‐prone landscapes is being evaluated for wildfire mitigation. Consequently, the study of the ecological value of coarse woody debris to sustain dependent species has become of foremost importance. From 2004–2009, we used a before‐after control‐impact (BACI) study design to assess the effects of downed wood removal on a population of big‐eared woodrats (Neotoma macrotis) in an oak woodland (Quercus spp.) in coastal‐central California, USA. Using Pollock's robust design mark‐recapture analyses (with 12 …

High Thermal Quality Rookeries Facilitate High Thermoregulatory Accuracy In Pregnant Female Rattlesnakes, Haley A. Moniz, Jack Henry Buck, Hayley L. Crowell, Scott M. Goetz, Trevor D. Ruiz, Emily N. Taylor, Scott M. Bobacks

United States Department of Agriculture Wildlife Services: Staff Publications

Temperature is a primary factor influencing organismal development, and the fluctuating daily and seasonal thermal regimes of temperate climates may challenge the ability of viviparous reptiles to optimize body temperatures during gestation. Testing how viviparous reptiles navigate highly variable thermal conditions (e.g., relatively cold nights and/or highly fluctuating temperatures) is a powerful way to understand how they use microhabitats for thermoregulatory benefits. We assessed the thermal ecology of pregnant and non-pregnant female prairie rattlesnakes (Crotalus viridis) inhabiting a high-elevation, montane shrubland in northwest Colorado throughout their short summer active season, addressing the thermal consequences of microhabitat selection with …

Characterizing Feral Swine Movement Across The Contiguous United States Using Neural Networks And Genetic Data, Rachael M. Giglio, Courtney F. Bowden, Ryan K. Brook, Antoinette J. Piaggio, Timothy J. Smyser

United States Department of Agriculture Wildlife Services: Staff Publications

Globalization has led to the frequent movement of species out of their native habitat. Some of these species become highly invasive and capable of profoundly altering invaded ecosystems. Feral swine (Sus scrofa × domesticus) are recognized as being among the most destructive invasive species, with populations established on all continents except Antarctica. Within the United States (US), feral swine are responsible for extensive crop damage, the destruction of native ecosystems, and the spread of disease. Purposeful human-mediated movement of feral swine has contributed to their rapid range expansion over the past 30 years. Patterns of deliberate introduction of …

Bayesian Integrated Species Distribution Models For Hierarchical Resource Selection By A Soaring Bird, Ryo Ogawa, Guiming Wang, L. Wes Burger, Bronson Strickland, J. Brian Davis, Fred L. Cunningham

United States Department of Agriculture Wildlife Services: Staff Publications

Migratory birds exhibit seasonal geographic range (hereafter, range) dynamics during the annual cycle. Few studies have examined how migratory birds select their habitats for range occupancy at the species level and space use at the individual level simultaneously. We hypothesized that environmental variables directly related to fitness components would affect the range occupancy probabilities of migrants, whereas environment variables related to movements and flights would affect the space use intensities of migrants. We built Bayesian integrated species distribution models (ISDMs) to evaluate the effects of climate conditions, wind conditions, and landcover compositions on the seasonal range dynamics of American white …

Raccoon Density Estimation From Camera Traps For Raccoon Rabies Management, Amy J. Davis, Wesley C. Dixon, Richard B. Chipman, Amy T. Gilbert, Jacob E. Hill, James C. Beasley, Olin E. Rhodes Jr., Guha Dharmarajan

United States Department of Agriculture Wildlife Services: Staff Publications

Density estimation for unmarked animals is particularly challenging, yet density estimates are often necessary for effective wildlife management. Raccoons (Procyon lotor) are the primary terrestrial wildlife reservoir for Lyssavirus rabies within the United States. The raccoon rabies variant (RRVV) is actively managed at landscape scales using oral rabies vaccination (ORV) within the eastern United States. To effectively manage RRVV, it is important to know the density of raccoons to appropriately scale the density of ORV baits distributed on the landscape. We compared methods to estimate raccoon densities from camera‐trap data versus more intensive capture‐mark‐recapture (CMR) estimates across 2 …

Aged 539 Internship Report: Improving Cal Poly Rodeo Horses' Health And Fitness, Faith Star Mahallati

Agricultural Education: Graduate Internship Reports

This study was conducted to help enhance the overall health and fitness of the horses on the Cal Poly Rodeo Team. Data was collected before and after implementing the StallGrazer feeders to reduce the risk of sand colic and improve feed efficiency. Data was collected and analyzed before and after the weekly barrel racing jackpot series to enhance horse fitness. The StallGrazer feeders reduced the risk of sand clear and forage waste and the barrel racing jackpots enhanced the fitness and performance of the horses in the test group.

8th Cauthen Seminar (2024) Program, Cauthen-Seminar

Veterinary Science Presentations

Contained here is the complete program for the 8th Annual Cauthen Seminar held at the Gluck Equine Research Center.

The Cauthen seminar series was started in 2017 as a tribute and memorial to respected and beloved farrier and teacher, “Tex” Cauthen, developed to encourage the sharing of best practices, technology, research, experience, and insight among all communities in the equine industry, from owners, farriers, track managers, veterinarians, and jockeys.

The annual series has been hosted at the Gluck Equine Research Center at the University of Kentucky in Lexington, KY along with a live broadcast as a Zoom webinar, offered free …

2024 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow- calf Nutrition, Reproduction, and Management

• Impact of Increasing Level of Milk Production on Cow- Calf Performance in Nebraska Sandhills
• Artificial Insemination of Beef Heifers with Multi- Sire Semen
• Effect of Heifer Percent Mature Body Weight at Breeding on Heifer Performance, Calf Production, and Subsequent Pregnancy Rates
• Effect of Glucogenic Feed Additive on Reproductive Performance in Young Postpartum Range Cows
• The Effect of Late Gestation Supplementation Strategy on Cow- Calf Performance in March- Calving Mature Cows
• The Impact of Pre- partum Supplementation Strategy on Cow- Calf Performance in May- Calving Mature Cows
• The Effect of Postpartum Supplementation Strategy on Performance …

Predicting Live Body Wight Of Yearling Beef Heifers Using 3d Imaging, Dalton J. Anderson, Yijie Xiong, Andrea K. Watson, J. Travis Mulliniks

Nebraska Beef Cattle Reports

This study was conducted to determine the accuracy of using 3D imaging technology as a method to predict shrunk body weight (BW) of growing yearling beef heifers. Red Angus x SImmental heifers (n = 69, BW = 726 ± 62 lbs; 12 months of age) were utilized for data collection. A time-of-flight depth camera (Azure Kinect, Microsoft) was used to collect depth videos as heifers walked out of the chute. Ideal image frames were identified from videos and used to determine the body volume of each heifer. Prediction of BW using images produced an R² (estimate of model fit) …

Impact Of Varying Inclusion Of Modified Distillers Grains Plus Solubles Compared To Constant Inclusion On Feedlot Cattle Performance And Carcass Characteristics, Debora G. Sousa, Rebecca Mcdermott, Mitch M. Norman, J. C. Macdonald, Rick A. Stock, Galen E. Erickson

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the effect of varying inclusion of modified distillers grains plus solubles on a weekly basis with two inclusions of grass hay on the performance of finishing steers. no interaction was observed between modified distillers grains plus solubles inclusion (0%, 25% constant, or 25% varying from 15-35%) and hay inclusion (6% or 12%). When evaluating the effect of hay inclusion on performance, cattle fed 6% grass hay had less dry matter intake than those fed 12% grass hay, and there was a tendency for gains to be greater for cattle fed 6% grass hay. Adding …

Impact Of Feeding New Fractioinated Distillers Grains (Fiber Plus Syrup) On Feedlot Cattle Performance And Carcass Characteristics, Debora G. Sousa, Rebecca L. Mcdermott, Mitch M. Norman, James C. Macdonald, Rick Stock, Ryan A. Mass, Galen E. Erickson

Nebraska Beef Cattle Reports

Fractionation processes are being explored to supply higher protein distillers grains for premium markets. Resulting distillers grains after a fraction is isolated will still be marketed as a cattle feed so knowing the impact on performance is important. This study evaluated feeding wet or dry conventional distillers grains with wet and dry fractionated distillers (fiber plus syrup) fed at 0, 20, or 40% of diet DM. When compared to the corn control, intake and gain increased for each of the four distillers types were fed, but feed conversion was poorer (i.e., increased) for dry distillers grains and dry fiber plus …

Comparison Of Corn Silage And Earlage In Finishing Diets When Fed As A Roughage On A Neutral Detergent Fiber Basis, Garrett N. Lemon, Rebecca Mcdermott, Alfredo Dicostanzo, Galen E. Erickson, Pablo L. Loza

Nebraska Beef Cattle Reports

The finishing trial was conducted to evaluate the ability of earlage to serve as a roughage source compared to corn silage when both sources were balanced on an NDF basis. Steers were fed in a randomized block design with a 2x2 treatment arrangement, with one factor being NDF source (corn silage or earlage) and the other factor being inclusion ("normal" amount of roughage provided by corn silage or earlage as the only grain source and roughage source). No significant interactions were observed between roughage source and inclusion level on steer performance. Steers fed corn silage and grain had greater intakes …

Nebraska Producer Perceptions On The Role And Implications Of Negotiations In Fed Cattle Transactions, Taralee Hudson, Elliott James Dennis, Brad D. Lubben, James C. Macdonald

Nebraska Beef Cattle Reports

A survey of ten feelot operators, as identified through the Nebraska Department of Agriculture's (NDA) 2019-2020 Cattle Feeder's Directory was conducted to identify the important concepts related to negotiated transactions and price discovery within the fed cattle market. The surveyed feedlots account for approximately 10 percent of the cattle on feed identified within the directory, on a one time capacity basis. Conducted in April of 2023 via phone conversation, the questions were related to 1) negotiated cash transactions, 2) the bidding process, and 3) thinning cash trade. Such results lend insight into the marketing practices that currently dominate the fed …

Evaluation Of Gas Emissions From Cattle On Different Diet Adaptation Strategies Using Either Forage Or Ramp, Cindy D. Mansfield, Rebecca Mcdermott, Braden C. Troyer, Mitchell M. Norman, Maggie Youngers, Andrea K. Watson, Rick Stock, Galen E. Erickson

Nebraska Beef Cattle Reports

A 173-day finishing experiment was conducted to evaluate the effects of feeding RAMP (Cargill Corn Milling, Blair, NE) during diet adaptation compared to a traditional forage adaptation program on methane and carbon dioxide emissions, animal performance and carcass traits in beef steers. Cattle were monitored using a calorimetry emission barn to quantify production of methane and carbon dioxide during step 1 of grain adaptation and at two subsequent times while fed a common finishing diet. Feeding RAMP reduced methane by 12% during the initial diet (step 1) compared to a traditional diet that contained 43% forage. When cattle were fed …

Rethinking Corn Residue: Effects Of Grain Yield On Quality And Quantity Of Residue, Kaylee E. Wheeler, Jessica Miller, Sadie Ference, Stephan Geu, Bill Struckmeyer, Amanda Easterly, Cody F Creech, Brian Maust, Daren D. Redfearn, Jay Parsons, Mary E. Drewnoski

Nebraska Beef Cattle Reports

A two-year experiment evaluated the effects of corn grain yield on the resulting quality and quantity of corn residue. Among the wide variety of corn hybrids, locations, and growing conditions, observed grain yields ranged from 120 to 350 bushels per acre. As corn yield increased, the total pounds of residue increased. However, the amount of reside relative to grain decreased. Within the residue, the proportion of leaf increased from 10 to 28% as corn yield increased while husk remained unchanged at 13%. Overall, the yield of leaf and husk per bushel of corn grain was not affected by grain yield …

Survey Of Current Management Practices And Evaluation Of Their Impact On Nutrient Content Of Small Cereal Silage In Nebraska, Alexa R. Johnson, Mary E. Drewnoski, Daren D. Redfearn, Susan Vanderplas, Jenny Rees, Ben Beckman, Erin Laborie, Todd Whitney, Kim Clark, Brad Schick, Gary Lesoing, Conner Biehler

Nebraska Beef Cattle Reports

The nutrient content of small cereal silage from 16 producers in Nebraska was measured at harvest and post-fermentation. At packing, 42% of the samples were below the target dry matter of 30-35%. Samples with dry matter percentage below 30% had a significant increase in the loss of energy (total digestible nutrient) content of the silage. The wetter silage appeared to have increased rates of clostridial fermentation as indicated by production of butyric acid. These data suggest that moisture management is a challenge and increased attention to ensuring the target dry matter content is achieved before packing could improve the quality …

Cereal Rye, Winter Triticale Or Winter Wheat: Which Is Best For Early Spring Grazing?, Alyssa K. Hall, Abigail M. Sartin, Kallie J. Calus, Morgan T. Grabau, Daren D. Redfearn, Robert B. Mitchell, Mary E. Drewnoski

Nebraska Beef Cattle Reports

A study was conducted to determine which winter-hardy small cereal grain was best suited for early spring grazing. Three species were evaluated: winter wheat, cereal rye, and winter triticale, as a double-cropped forage in a continuous soybean rotation. Within this rotation the number of grazing days is limited, but all three species provided high rates of cattle weight gain, with an average daily gain (ADG) of 3 lb/d in April. However, in a year where freezing conditions occurring after cattle started grazing, cattle grazing cereal rye had the greatest ADG, likely due to greater forage growth. Thus, cereal rye may …

Vitamin A In Cow-Calf Production: Impacts Of Maternal Supplementation And Status On Offspring, Hannah F. Speer, Harvey C. Freetly, Karla H. Jenkins, Mary E. Drewnoski

Nebraska Beef Cattle Reports

The young calf is at greatest risk of vitamin A deficiency when cow vitamin intake is low in late pregnancy. Two studies were conducted to evaluate the relationship between cow and calf vitamin A status and how vitamin A status of cow-calf pairs was influence by maternal vitamin A supplementation. In general, calves did not have adequate liver vitamin A concentrations despite cows having adequate liver vitamin A stores following calving. Both cow iver stores and cow vitamin A intake during late gestation influence the amount of vitamin A in colostrum, so it benefits the calf if the cow has …

The Effect Of Postpartum Supplementation Strategy On Performance In May-Calving 2- And 3-Yr-Old Range Cows, Nicole M. Woita, Selby L. Boerman, J. A. Musgrave, Kacie L. Mccathy, J. Travis Mulliniks

Nebraska Beef Cattle Reports

Reproductive performance in young 2- and 3-yr-old cows are often the lowest in the cow herd, which is due to their inability to consume enough energy and protein to meet their requirements for growth and lactation. A 3-yr study was conducted to evaluate the effects of postpartum supplementation strategy on reproduction, cow body weight, and calf performance in lactating young 2- and 3-yr-old May-calving range cows. Supplementation was initiated 20 d prior to the start of the breeding season (45-d postpartum) and continued throughout the 45-d breeding season (125-d postpartum). Supplementation was provided daily with treatments being 1) mineral supplement …

The Impact Of Prepartum Supplementation Strategy On Cow-Calf Performance In May-Calving Mature Cows, J. A. Musgrave, Kacie L. Mccathy, J. Travis Mulliniks

Nebraska Beef Cattle Reports

Implementation of minimal supplemental protein strategies during late gestation may have the potential to minimize any negative postweaning progeny performance while decreasing feed costs. A 3-yr study was conducted to evaluate the effects of mid- to late-gestation supplementation strategies on reproduction, cow body weight, and calf performance in May-calving mature range cows grazing upland native range. Supplementation was provided 2x weekly with treatments being 1) 05 lb per day of a 30% CP distillers-based supplement, 2) 1 lb per day of a 30% CP distillers-based supplement, 3) a negative control as a flexible supplementation strategy. The Flex strategy was developed …