Veterinary Medicine Commons^™

Surveillance Of Highly Pathogenic Avian Influenza Virus In Wild Canids From Pennsylvania, Usa, Kevin D. Niedringhaus, Taylor C. Chan, Ashley Mcdowell, Lauren Mxwell, Madison Stevens, Lane Potts, Erica Miller, Eman Anis, Kyle Van Why, Thomas Keller, David Stallknecht, Rebecca L. Poulson, Kaitlyn Bahrs, Justin D. Brown

United States Department of Agriculture Wildlife Services: Staff Publications

The avian influenza virus is a global pathogen with significant health and economic implications. While primarily a pathogen of wild and domestic birds, recent outbreaks of the H5N1 highly pathogenic avian influenza virus (HPAIV) clade 2.3.4.4b have caused mortality in a wide variety of mammals, including members of the Canidae family, on multiple continents. Despite sporadic mortality events globally, the epidemiology and pathobiology of H5N1 HPAIV in wild canids remains poorly defined. During 2022–2024, 41 wild canid carcasses (diagnostic cases), including 23 red foxes and 18 gray foxes, were tested for the influenza A virus (IAV) via PCR, with five …

Rabies, Amy T. Gilbert, Christine Ellis

United States Department of Agriculture Wildlife Services: Staff Publications

Illustrated fact sheet on rabies.

Effect Of Haemonchus Contortus Excretory/Secretory Protein On Differences In Host Neutrophil Migration, Hannah Grace Teddleton

Graduate Theses, Dissertations, and Problem Reports (ETD)

An excretory/secretory (E/S) product, common to many helminths, is neutrophil inhibitory factor (NIF) which negatively affects neutrophil migration and activity. Due to differences in neutrophil accumulation between parasite resistant St. Croix STC and parasite susceptible Suffolk (SUF) sheep, we hypothesized that Hc-NIF may inhibit migration of SUF and not STC neutrophils. Previous studies have demonstrated that Hc produces NIF but is not commercially available. To test our hypothesis, neutrophils were cultured from STC and SUF sheep in the presence of Ancylostoma caninum-derived NIF (Ac-NIF) and measure chemotaxis to Interleukin-8 (IL-8), HcLA or H. contortus third-stage excretory/secretory (E/S) products. Neutrophils were …

Anatomical And Histological Features Of The Brain Of Adult Common Carp (Cyprinus Carpio), Sayed Shaban, Galal Youssef, Safwat Ebada, Mahmoud B. Shoeib

Mansoura Veterinary Medical Journal

Background: investigated the anatomical features and histological structures of the brain of adult common carp. In addition, these features were compared with those of other types of fish.

Methods: Adult fish (8- 18 M) were collected, their gross anatomical features were inspected; and small specimens from different brain parts were fixed, sectioned, stained, and histologically examined.

Results: In the common carp, the olfactory bulb was round, somewhat tiny, and connected to the telencephalon by a very long, thin olfactory tract. The hemispheres of the telencephalon were located rostrally. Only one median ventral telencephalic ventricle was visible. The torus longitudinalis and …

Molecular Investigation Of Tick-Borne Pathogens In Feral Swine (Sus Scrofa) From Seven Us States, Sofiane Aiche

Masters Theses

Feral swine (Sus scrofa) have been proposed as part of the lifecycles of multiple tickborne pathogens (TBP). This is a result of the increase in feral swine populations and increased tick populations and geographic ranges. Few surveys exist on the TBP relevant to human and wildlife of feral swine in the US. The aim of this study is to investigate the prevalence of 10 TBP: Anaplasma spp., Borrelia spp., Cytauxzoon felis, Hepatozoon spp., Babesia spp., Mycoplasma spp., Trypanosoma cruzi, Toxoplasma gondii, Yersinia pestis, and Rickettsia spp. A total of 87 feral swine blood samples …

Abelson (Abl) Tyrosine Kinase Inhibitors Reduce Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication In Vitro, Jenna Parliament

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped, positive-sense, single-stranded RNA virus from the Arteriviridae family. PRRSV causes respiratory illness and reproductive failure in infected pigs. Outbreaks occurring in the United States and worldwide cause significant economic losses annually. Abelson (Abl) tyrosine kinase is a non-receptor tyrosine kinase, which plays a central role in regulating many cellular processes such as actin reorganization, cell proliferation and differentiation by transducing extracellular and intracellular signals. Abl inhibitors have been shown to restrict the entry of membrane fusion of enveloped viruses, such as severe acute respiratory syndrome coronavirus, Middle Eastern respiratory syndrome …

Determination Of Yield Nutrient Contents And In Vitro Digestibility Of Some Triticale Varieties Harvested At Different Maturity Stages, Şevket Evci̇, Kami̇l Kara, Mehmet Demi̇rci̇

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the contribution of growth performance, nutrient content and digestibility of different triticale varieties to ruminant diets and to indirectly assist farmers in feed production. The experiment utilized 5 triticale varieties. The study was designed as a randomized complete block design with split plots and 3 replications. The digestibility values were determined in vitro after 48 hours of incubation using the Daisy Ankom® digester. Rumen liquid was obtained from dairy cows slaughtered at the 'Yeşil Vadi Et Entegre' facility. Nutrient values, in vitro dry matter digestibility, and yield traits of forages were determined. …

Predictors On Mental Health Of Owning Cats And Dogs, Çağri Çağlar Si̇nmez, Ahmet Meti̇n, Emre Tüfekçi̇, Ali̇ İlteri̇ş Aykun, Vehbi̇ Güneş

Turkish Journal of Veterinary & Animal Sciences

The common consensus is that owning pets affects human health positively. However, the effects of owning pets on mental health are still controversial. Therefore, the present study aimed to determine the possible associated with depression, anxiety, stress, and owning cats and dogs in Türkiye. Additionally, the potential impact of personality traits on this relationship was examined. This study included 636 participants, of whom 52% were female (Mage = 33.02, SD = 15.78). Data was obtained by using a DASS-21 and the Big Five Personality Inventory. The findings revealed that owning cats and dogs was a promotive factor against depression (DE) …

Molecular Characterization Of Fecundity-Related Gene Regions In Some Of Türkiye’S Native Sheep Breeds, Ramazan Aymaz, Fulya Özdi̇l, Yalçın Yaman

Turkish Journal of Veterinary & Animal Sciences

In this study, the BMP15 and GDF9 gene regions were examined through DNA sequencing in some native sheep breeds of Türkiye (Kıvırcık, Karacabey Merino, Chios, Gökçeada, Çine Çaparı, Awassi, and Karakacan). In the BMP15 exon 2 region, two single nucleotide polymorphisms (SNPs) were identified: one synonymous SNP, P248P (proline/proline), and one nonsynonymous SNP, L251P (leucine/proline), resulting in a missense mutation. In the GDF9 exon 1 region, only a nonsynonymous SNP, R87H (histidine/ arginine), was found. In the GDF9 exon 2 region, four SNPs were identified: two nonsynonymous SNPs, E241K (glutamate/lysine) and V332I (valine/isoleucine), and two synonymous SNPs, L159L (leucine/leucine) and …

Behavioral Activity Budget Of Grower Beetal Kids Offered Total Mixed Ration Under Stall-Fed System, Priya Dhattarwal, Mandeep Singla, Sandeep Kaswan, Ravinder Singh Grewal, Neeraj Kashyap, Dalpat Singh Malik

Turkish Journal of Veterinary & Animal Sciences

This study investigates the behavioral activities of grower Beetal kids fed in a conventional method-control group manner orgiven total mixed ration (TMR) (i.e. hay-based - T1 and green fodder-based - T2). Twenty-four weaned Beetal kids were enrolled andrandomly distributed into 3 groups based on their body weight, sex, and age. Behavioral observations were recorded 30 min after givingexperimental ration to the kids using the scan sampling method for 1 h, which inferred time spent eating normally, eating with forelegson the cage, and eating with forelegs on the feeders; standing idle and urination were significantly higher in the control group than …

Identification Of Dermatophytes And Comparative Efficacy Of Topical Antifungals Fortreating Canine Dermatophytosis, Arun Prakash Vadakkoot, Rathish Ramachandran Latha, Neeraja Eramthottil, Lijo John, Bipin Kuriyedath Chitharalil, Deepa Padinjare Meleppat

Turkish Journal of Veterinary & Animal Sciences

Dermatophytosis is a potentially zoonotic superficial skin infection caused by keratinophilic fungi. Identification ofdermatophytes aids in understanding the epidemiology of infections and finding out better treatment modalities. The study aimed toidentify dermatophytes in dogs and compare the effectiveness of three different topical drugs for treating dermatophytosis. Molecularidentification of dermatophyte isolates obtained on culturing was done by restriction fragment length polymorphism of the ampliconobtained by polymerase chain reaction of the Internal Transcribed Spacer (ITS) region of the recombinant DNA. Thirty of the positivecases were divided into three groups, Group I was treated with shampoo containing 2% each of miconazole chlorhexidine, Group …

The Stereological And Morphometrical Investigation Of Pars Lumbalis Of The Spinal Cord Innew Zealand Rabbit, Berna Duman, İftar Gürbüz

Turkish Journal of Veterinary & Animal Sciences

In the study, 12 (6 females, 6 males) healthy adult New Zealand rabbits were used to examine the morphometrical andstereological values. White matter, gray matter and total segment volume of the segments in the pars lumbalis were calculated byCavalieri’s principle. The findings were grouped according to sexand statistically evaluated. As a result, 38% of the length of the spinalcord in rabbits belonged to the pars lumbalis. The morphometric parameters were not statistically different between male and femalerabbits (p > 0.05). The segment with the biggest volume was L5. White matter’s greatest volume was the L4 segment. The volume of graymatter was …

Determining The Effects Of Different Bypass Fat Supplementation On Performance, Milkyield, And Milk Composition Of Anatolian Buffaloes, Sezer Öz, Seher Küçükersan

Turkish Journal of Veterinary & Animal Sciences

This study aimed to explore the effects of animal bypass fat (ABF) and palm bypass fat (PBF) added to the rations of Anatolianbuffaloes on dry matter consumption, feed conversion ratio, milk yield, some milk components, and parameters. A total of 21 heads ofbuffalo in the early lactation period; milk yield averages, lactation periods, lactation numbers, live weights, and ages were selected anddivided into three experimental groups, each consisting of 7 buffaloes. The experiment was conducted according to the crossover trialpattern. There was no statistically significant difference between the groups in terms of dry matter consumption and feed conversionratio (p > 0.05). …

Efficacy Of Bacillus-Based Probiotic And Nspase Supplementation On Growth Performance And Gut Health Of Broilers Fed High Level Of Palm Kernel Meals Under High Stocking Density, Nurul Imawati

Chulalongkorn University Theses and Dissertations (Chula ETD)

Palm kernel meal (PKM) is a by-product of the oil palm industry with significant potential in poultry feed, although its high content of anti-nutritional factors limits its utilization. It is hypothesized that dietary supplementation with fiber-degrading enzymes and probiotics may enhance the use of PKM. This study investigated the effects of Bacillus-based probiotics (PRO) and non-starch polysaccharide enzymes (NSPase, mannanase, and xylanase) on broiler performance and gut health when fed a high level of PKM under high stocking density (HSD, Experiment 1) and normal stocking density (NSD, Experiment 2). In Experiment 1, 4,480 mixed-sex Ross308 broilers were allocated to eight …

The Development Of A Lateral Flow Immunochromatographic Strip Test For Evaluation Of Specific Antibody Levels Against Porcine Epidemic Diarrhea Virus In Pig Milk, Patumporn Jermsutjarit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine epidemic diarrhea virus (PEDV) causes severe watery diarrhea in pigs of all ages, but it is particularly devastating in neonatal piglets, where it can result in 100% mortality. Therefore, lactogenic immunity is crucial for protecting against PEDV infection and reducing its virulence. One method for controlling PEDV outbreaks involves using feedback as a protocol, which entails inoculating pregnant sows with infected-PEDV intestines from piglets to enhance lactogenic immunity against PEDV. Interestingly, high serial passage of PEDV has been shown to reduce the virus's virulence. To understand the effects of serial passage, the PEDV was serially passaged both in vitro …

The Development Of A Differentiating Of Infected From Vaccinated Animals (Diva) Elisa To Detect Antibody Against Senecavirus A, Parin Watcharavongtip

Chulalongkorn University Theses and Dissertations (Chula ETD)

Senecavirus A (SVA) is the causative agent of porcine idiopathic vesicular disease (PIVD), a condition clinically indistinguishable from other vesicular diseases in pigs, such as foot and mouth disease (FMD). This similarity complicates differential diagnosis, impacting the global swine industry, including Thailand. Rapid and accurate diagnostic methods are essential for effective disease control. Therefore, developing a highly sensitive and specific diagnostic tool for detecting SVA infection is crucial for distinguishing it from other vesicular diseases and controlling its spread. In this study, we conducted a retrospective analysis of SVA emergence in diagnostic samples from 2010 to 2021 in Thailand, with …

Development Of An Endometrial Cell Culture System Of Thai Swamp Buffalo (Bubalus Bubalis) For Studying The Endometrial-Embryo Interactions, Ruchikon Jongsuwanwattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Developing and optimizing endometrial sampling techniques are critical for advancing reproductive management and research in cattle and swamp buffalo. This PhD thesis aims i) to design a novel endometrial sampling device for cows and swamp buffaloes, ii) to develop a minimally invasive endometrial cell sample collection technique in cows and buffaloes to prepare endometrial cell culture, iii) to validate the developed mixed endometrial cell culture system retrieved by brush cytology techniques in cows and swamp buffaloes and iv) to utilize the minimally invasive endometrial cell sampling technique for investigation of reproductive disease in ruminant herds. The research is consequently structured …

Proteomic Analysis Of Saliva, Serum And Tissue In Feline Mammary Tumors Using Maldi-Tof Mass Spectrometry And Lc-Mass Spectrometry/Mass Spectrometry, Weejarin Paphussaro

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline mammary carcinomas (FMC) are commonly found in female cats. Early detection is necessary to prevent both local and distant metastasis and increase survival times. However, the current protein biomarkers of FMC are limited. Several biomarkers of cancers have been discovered by mass spectrometry (MS)-based proteomics, such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and liquid chromatography-mass spectrometry/ mass spectrometry (LC-MS/MS) which high sensitivity and accuracy for identified biomarker in various cancer. The purpose of this study was to identify protein expression in saliva, serum and tissue in FMC using MALDI-TOF MS and LC-MS/MS. Results of …

Molecular Characterization And Immunohistochemical Investigation Using Anti-Capsid Protein Antibody Of Canine Circovirus Associated With Respiratory Diseases In Thai Dogs, Wichan Dankaona

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine circovirus (CanineCV) is an emerging virus linked to various diseases in dogs, especially respiratory and gastrointestinal ailments. This thesis focuses on molecular characterization and histopathological investigation of CanineCV in dogs from Thailand, aiming to provide insights into the role of virus in canine respiratory diseases. The study involved genomic-based surveillance of CanineCV from nasal and oral swabs of infected dogs by employing polymerase chain reaction (PCR) assay. Additionally, the in situ hybridization (ISH) technique was used to identify and localize the viral genome within infected tissues. The CanineCV recombinant capsid gene (rCap) and protein (rCap) were synthesized and characterized, …

Impacts Of Pcv3 And Pcv2 On Reproductive Performance In The Vietnamese Industrial Sow Herds, Tien Tran Nam

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine circovirus (PCV) 2 is a globally prevalent virus causing negative impacts on the swine industry. Controlling PCV2 with vaccines is a mandatory requirement in the farming procedures depending on the prevalence of the virus and farm management system including vaccination program. It should be noted that PCV2d management in the studied farms appeared under control via the effective vaccination program. Since the first descriptions of PCV3 in the US, many publications on the viral pathogenesis and effects on susceptible animals have been conducted, but complete consensus has not been reached. Therefore, an alternative approach to studying the virus is …

Use Of Ultrasonography For Evaluation Of Response To Chemotherapy In Canine Multicentric Lymphoma, Somchin Sutthigran

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine multicentric lymphoma (CML) is a common hematopoietic neoplasm in dogs that initially responds well to treatment but often relapses due to chemotherapy resistance. Evaluating the treatment response is a critical factor in helping veterinary oncologists plan and adjust chemotherapeutic protocols. This study aims to investigate the use of basic diagnostic imaging techniques, such as ultrasound (US), advanced imaging, such as computed tomography (CT) and hematologic and blood chemistry parameters, to enhance the evaluation of treatment response for this disease. This prospective observational study included 95 lymphomatous lymph nodes (LLNs) from 15 dogs with CML and 60 normal lymph nodes …

Vaccination Against Streptococcus Iniae And Bacillus Probiotic For Health Enhancement In Juvenile Barramundi (Lates Calcarifer), Pornpawit Tanpichai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Juvenile Asian seabass (Lates calcarifer) with an average body weight of 10 ± 0.7 g were used to investigate the efficacy of an inactivated Streptococcus iniae vaccine and the probiotic Bacillus subtilis. Fish were intraperitoneally vaccinated with 1012 CFU fish−1 of formalin-killed S. iniae (V) or sham vaccinated (S) with sterile normal saline solution (NSS). Both groups were fed with Bacillus probiotic (5 × 109 CFU kg-1 diet) for 6 weeks. The protective efficacy of the vaccine against S. iniae examined at 4, 10 and 20 weeks post-vaccination showed that vaccinated (V) and vaccinated and probiotic (VP) groups had no …

The Impact Of Cow Behaviors During Transition Period On Reproductive And Milk Productive Performance, Aqeel Raza

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigates the impact of behavioral patterns during the transition and early postpartum periods on reproductive and lactation outcomes in tropical dairy cows, using the AfiTag-II biosensor to monitor activity, rest time, rest per bout, and restlessness ratio. Data from 298 prepartum and 347 postpartum Holstein Friesian cows, categorized by lactation number (1, 2, and ≥3), was analyzed. K-means clustering on 227 lactation records identified distinct behavioral groups, facilitating the assessment of reproductive traits through Cox regression and lactation curves using the Wood model. First-lactation cows had shorter prepartum rest durations (568.8 ± 5.4 min/day) compared to higher-parity cows, …

Genetic Characteristics Of Salmonella Carrying Plasmid-Associated Antimicrobial Resistance And Virulence Genes Isolated From Pigs, Pork, And Humans In The Bordering Provinces Between Thailand And Some Land Neighboring Countries, Siraphatson Wetchasirigul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Antimicrobial resistance (AMR) is a major public health threat. The issue has disproportionately affected in low- and middle-income countries including Thailand and its neighboring counties where high rates of resistant Salmonella have been continuously reported in pigs, pork, and humans. Recognizing the significant role of R plasmids in AMR distribution, knowledge on virulence genes associated plasmids is still limited. The frequency and patterns of virulence factors are still not routinely tracked. This study aimed to characterize Salmonella enterica isolates carrying plasmid-associated virulence and AMR genes from pig, pork, and human isolates in border provinces of Thailand, Lao PDR, Cambodia, and …

Candidate Circulating Microrna Biomarkers In Dogs With Chronic Pancreatitis, Susan K Armstrong, Robert W Hunter, Wilna Oosthyuzen, Maciej Parys, Adam G Gow, Silke Salavati Schmitz, James W Dear, Richard J Mellanby

Faculty, Staff and Student Publications

BACKGROUND: Pancreatitis is an important cause of disease and death in dogs. Available circulating biomarkers are not sufficiently sensitive and specific for a definitive diagnosis.

HYPOTHESIS: Circulating microRNAs would be differentially expressed in dogs with chronic pancreatitis and could have potential as diagnostic biomarkers.

ANIMALS: Healthy controls (n = 19) and dogs with naturally occurring pancreatitis (n = 17).

METHODS: A retrospective case-control study. Dogs with pancreatitis were included if they satisfied diagnostic criteria for pancreatitis as adjudicated by 3 experts. MicroRNA was extracted from stored serum samples and sequenced. Reads were mapped to mature microRNA sequences in the canine, …