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2022

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Articles 301 - 330 of 414

Full-Text Articles in Veterinary Medicine

Investigating Genetic Diversity Of Indonesian Native Cattle Breeds Using Mitochondrialdna 16s Rrna Gene, Restu Misrianti, Sony Hartono Wijaya, Cece Sumantri, Jakaria Jakaria Jan 2022

Investigating Genetic Diversity Of Indonesian Native Cattle Breeds Using Mitochondrialdna 16s Rrna Gene, Restu Misrianti, Sony Hartono Wijaya, Cece Sumantri, Jakaria Jakaria

Turkish Journal of Veterinary & Animal Sciences

This study aimed to estimate the genetic diversity and phylogenetic relationships of Indonesian native and local cattle breeds using 16S rRNA mitochondrial DNA. The study analyzed 93 DNA samples from six cattle breeds. The polymerase chain reaction was conducted using two primer pairs to sequence the complete 16S rRNA gene. The number of the polymorphic site (S), pairwise nucleotide differences (K), haplotype diversity (Hd), and nucleotide diversity (?) were estimated using DnaSP 6 software. PopART v. 1.7 software was employed for the median-joining network analysis, while a neighbor-joining tree was reconstructed based on the Kimura 2-parameter model using MEGA X …

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Forage Turnip (Brassica Rapa) Harvested In Different Phases Of Vegetative Stage Andensiled With The Additives Of Molasses And Barley And The Effects Of Additives On Silagequality, In Vitro Digestibility, And Energy Content, Serhat Yildiz, Suphi̇ Deni̇z, Fatma Özkan, Çağri Kale Jan 2022

Forage Turnip (Brassica Rapa) Harvested In Different Phases Of Vegetative Stage Andensiled With The Additives Of Molasses And Barley And The Effects Of Additives On Silagequality, In Vitro Digestibility, And Energy Content, Serhat Yildiz, Suphi̇ Deni̇z, Fatma Özkan, Çağri Kale

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the silage quality, in vitro digestibility and energy content of forage turnip (Brassica rapa) ensiled with molasses or ground barley. Forage turnip was harvested at three different stages: the beginning, middle and end of the flowering period, and it was ensiled with no additive (control), 5% molasses or 4% ground barley. In the trial, pH, nutrient contents, ammonia nitrogen (NH$_{3}$-N) contents, volatile fatty acid (VFA) contents, Fleig scores, in vitro digestibility and energy content were determined. It was observed that the effect of the vegetative stage on the difference between the groups was significant …

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Monensin Induces Apoptosis In The Liver Tissue And Primary Hepatocytes Of Chicks, Muhammet Yasi̇n Tekeli̇, Murat Kanbur Jan 2022

Monensin Induces Apoptosis In The Liver Tissue And Primary Hepatocytes Of Chicks, Muhammet Yasi̇n Tekeli̇, Murat Kanbur

Turkish Journal of Veterinary & Animal Sciences

In this study, monensin (MON) was investigated for its apoptotic and genotoxic effects on chick liver tissue and cytotoxic and apoptotic effects on chick primary hepatocytes isolated from perfused liver tissue. Western blotting and real-time PCR (qPCR) were used to determine the apoptotic effect on primary hepatocytes and liver tissue, and the comet assay was used to determine the genotoxic effect on liver tissue. MON decreased cell viability in primary hepatocytes with increasing concentrations. When administered at concentrations of 1 and 10 μM, MON increased the levels of apoptotic p53, caspase-3, and caspase-9, and downregulated the expression of the antiapoptotic …

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Do Horses Learn How To Reach For Feed Depending On The Time Of Day?, Agnieszka Ziemianska, Maciej Trojan, Konrad Bazewicz, Justyna Wojtas, Tomasz Próchniak, Iwona Rozempolska-Rucinska Jan 2022

Do Horses Learn How To Reach For Feed Depending On The Time Of Day?, Agnieszka Ziemianska, Maciej Trojan, Konrad Bazewicz, Justyna Wojtas, Tomasz Próchniak, Iwona Rozempolska-Rucinska

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to assess the ability of horses to learn how to open feeder boxes in a direction associated with the time of day. The study involved 10 horses. The first stage of the study consisted in learning how to complete the task. The second stage involved the test and was followed by a control stage. The results indicated the highest number of failed attempts in the leftward movement test. The mean time required by the horses to complete the task of the leftward opening (10.27 s) was twofold longer than in the rightward direction test …

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Activities And Mechanisms Of Oregano, Marjoram And Rosemary Essential Oils Against Malassezia Pachydermatitis Isolates From Canine And Feline Otitis, Stefanie Bressan Waller, Marcia Kutscher Ripoll, Anna Luiza Silva, Emanoele Figueiredo Serra, Tábata Pereira Dias, Vittória Bassi Das Neves, Luciéle Pereira De Melo, Patrícia Lindemann, Otávia De Almeida Martins, Angelita Dos Reis Gomes, Renata Osório De Faria, Mário Carlos Araújo Meireles, João Roberto Braga De Mello, Marlete Brum Cleff Jan 2022

Activities And Mechanisms Of Oregano, Marjoram And Rosemary Essential Oils Against Malassezia Pachydermatitis Isolates From Canine And Feline Otitis, Stefanie Bressan Waller, Marcia Kutscher Ripoll, Anna Luiza Silva, Emanoele Figueiredo Serra, Tábata Pereira Dias, Vittória Bassi Das Neves, Luciéle Pereira De Melo, Patrícia Lindemann, Otávia De Almeida Martins, Angelita Dos Reis Gomes, Renata Osório De Faria, Mário Carlos Araújo Meireles, João Roberto Braga De Mello, Marlete Brum Cleff

Turkish Journal of Veterinary & Animal Sciences

Malassezia pachydermatis is an opportunistic yeast found in the ear canal of small animals; however, the current azole-based therapy applied to it has failed to achieve clinical success due to the antifungal resistance. This issue has encouraged the studies in natural products, such as Origanum vulgare (oregano), Origanum majorana (marjoram) and Rosmarinus officinalis (rosemary) essential oils, although their mechanism of action remains unclear. Malassezia pachydermatis specimens deriving from otitis cases in dogs (n = 22) and cats (n = 2) were subjected to CLSI M27-A3. Sorbitol protection and ergosterol effect were analyzed to investigate their mechanism of action. Fungistatic (MIC) …

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The Effect Of Osteosubstitution By Platelet-Rich Autofibrin And Hydroxyapatite Ceramic With Ss-Tricalcium Phosphate On Biochemical Parameters Of Blood In Rabbits, Svitlana Shevchenko, Mykhailo Rublenko Jan 2022

The Effect Of Osteosubstitution By Platelet-Rich Autofibrin And Hydroxyapatite Ceramic With Ss-Tricalcium Phosphate On Biochemical Parameters Of Blood In Rabbits, Svitlana Shevchenko, Mykhailo Rublenko

Turkish Journal of Veterinary & Animal Sciences

The paper presents the results of a rabbit blood biochemical study after the substitution of simulated defects in spongy and compact bone tissue by platelet-rich fibrin and its combination with hydroxyapatite granules with ß-tricalcium phosphate. The aim of the work is to establish the biochemical features of reparative osteogenesis in the time of osteosubstitution by platelet-enriched fibrin and its combination with hydroxyapatite and ß-tricalcium phosphate. Bone defects (d = 3 mm) were modeled in rabbits from control and experimental groups (n = 12) under general anaesthesia. In the control group, a replacement was performed with autologous fibrin enriched with platelets …

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The Effect Of Microbial Inoculants And Molasses On Quality And In Vitro Digestibility Of Silages Prepared With Different Proportions Of Ryegrass And Hungarian Vetch, Gökhan Şen, Taşkin Erol, Kami̇l Kara, Mehmet Demi̇rci̇, Mehmet Aki̇f Karsli Jan 2022

The Effect Of Microbial Inoculants And Molasses On Quality And In Vitro Digestibility Of Silages Prepared With Different Proportions Of Ryegrass And Hungarian Vetch, Gökhan Şen, Taşkin Erol, Kami̇l Kara, Mehmet Demi̇rci̇, Mehmet Aki̇f Karsli

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to determine the effect of microbial inoculants and molasses on the quality and in vitro digestibility of the silages ensiled from the cultivation of different proportions of ryegrass-Hungarian vetch mixtures grown in Central Anatolian conditions. For this purpose, 5% molasses and 10 g/ton (1.25 × 10$^{11}$ CFU/g) inoculant were added to silages of ryegrass (Lolium multiflorum L.) containing 20%, 40%, 60%, and 80% Hungarian vetch (Vicia pannonica L.), under laboratory conditions. The prepared silages were opened at the end of 60 days and their physical analysis, chemical contents, fermentation parameters, in vitro digestibilities using …

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Effects Of Pueraria Lobata Leaf Powder As A Feed Additive On The Immune System And Growth Performance Of Broiler Chickens, Jianping Gong, Dong Wu, Mengchu Li, Xiaoyu Qiu, Ying Jiang, Jie Zhang, Wenping Huang Jan 2022

Effects Of Pueraria Lobata Leaf Powder As A Feed Additive On The Immune System And Growth Performance Of Broiler Chickens, Jianping Gong, Dong Wu, Mengchu Li, Xiaoyu Qiu, Ying Jiang, Jie Zhang, Wenping Huang

Turkish Journal of Veterinary & Animal Sciences

The present research assessed the effect of different levels of Pueraria lobate leaf (PLL) on growth performance, immunity, antioxidant capacity, and blood index in broiler feed. A total of 384 at 21 days old chicks were assigned to 1 of 4 dietary treatments to examine the benefits of basal diet, basal diet +1% PLL, basal diet +2% PLL and basal diet +3% PLL. The trial was divided into 2 time periods, including starter from days 21-49 and finisher from days 50-70. The addition of 2% and 3% PLL powder to diets increased total protein content, triglycerides, albumin, and low-density lipoprotein …

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Crude Glycerin And Waste Sesame Seed In The Diets Of Growing Lambs: Impacts On Growth Performance, Nutrient Digestibility, Ruminal Fermentation, Carcass Characteristics, And Meat Fatty Acid Profile, Bora Bölükbaş, İsmai̇l Kaya Jan 2022

Crude Glycerin And Waste Sesame Seed In The Diets Of Growing Lambs: Impacts On Growth Performance, Nutrient Digestibility, Ruminal Fermentation, Carcass Characteristics, And Meat Fatty Acid Profile, Bora Bölükbaş, İsmai̇l Kaya

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to investigate the effects of a combination of crude glycerin and waste sesame seed on the growth performance, rumen fermentation, carcass characteristics, and meat fatty acid profile of lambs. Twenty-four male Karayaka lambs with initial body weights of 26.5 ± 0.53 kg were randomly divided into four groups and raised in individual pens. For 70 days, lambs were fed ad libitum the one of four concentrates: concentrate without CG and WSS (control), concentrate with 10% CG (G), concentrate with 10% WSS (W), and concentrate with 5% CG and 5% WSS (GW). All diets …

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High Sensitivity Cardiac Troponin T (Hs-Ctnt) And I (Hs-Ctni) Levels In Dogs With Dirofilaria Immitis, Cemaletti̇n Ayvazoğlu, Şemi̇stan Kiziltepe, Ümi̇t Yaşar, Zehra Gül Yaşar, Pinar Demi̇r, Abuzer Acar Jan 2022

High Sensitivity Cardiac Troponin T (Hs-Ctnt) And I (Hs-Ctni) Levels In Dogs With Dirofilaria Immitis, Cemaletti̇n Ayvazoğlu, Şemi̇stan Kiziltepe, Ümi̇t Yaşar, Zehra Gül Yaşar, Pinar Demi̇r, Abuzer Acar

Turkish Journal of Veterinary & Animal Sciences

Dirofilaria immitis (D. immitis); is a parasitic zoonosis with a prevalence of 53.8%, whose intermediate host is mosquitoes (Anopheles, Aedes, Culex, Taeniorhychus, Mansonia and Armigenes), final hosts are carnivores (dog, cat, fox, etc.) and humans. The causative agent settles in the pulmonary arteries and causes pulmonary hypertension, which can progress to congestive heart failure. Cardiac troponins (cTn), which have recently been used in veterinary cardiology, are considered the gold standard because of their high sensitivity and specificity for myocardial injury. In the study, high-sensitivity cardiac troponin T (hs-cTnT) and high-sensitivity cardiac troponin I (hs-cTnI) levels in healthy …

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Effects Of Replacing The Protein Content Of Azolla Pinnata With Concentrate On Physiological And Blood Profiles Changes In Sahiwal Calves, Ninad Bhatt, Ramesh Chandra, Kotresh Prasad Chikkagoudara, Diksha Kandpal, Deepesh Bharat Misra, Nitin Tyagi Jan 2022

Effects Of Replacing The Protein Content Of Azolla Pinnata With Concentrate On Physiological And Blood Profiles Changes In Sahiwal Calves, Ninad Bhatt, Ramesh Chandra, Kotresh Prasad Chikkagoudara, Diksha Kandpal, Deepesh Bharat Misra, Nitin Tyagi

Turkish Journal of Veterinary & Animal Sciences

Eighteen female Sahiwal calves of average four month of age and 54 kg body weight were selected for the study. The animals were divided into three groups (T0, T1, and T2) on body weight basis. The animals were fed as per ICAR feeding standard (2013) for calves for 90 days. The animals in T0 group T0 were fed as per feeding standard while in T1 and T2 groups, fresh Azolla pinnata was fed by replacing 15% and 30% protein content of concentrate with Azolla pinnata on DM basis. The nonsignificant results were obtained for all the physiological parameters. In addition, …

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Determination Of Acrylamide Using The Immuno-Enzymatic Method In Commercial Dog And Cat Foods, Ni̇met Kiliç, Bi̇lal Cem Li̇man Jan 2022

Determination Of Acrylamide Using The Immuno-Enzymatic Method In Commercial Dog And Cat Foods, Ni̇met Kiliç, Bi̇lal Cem Li̇man

Turkish Journal of Veterinary & Animal Sciences

Pet animals raised by humans do not have the chance to choose their own food. Therefore, mistakes made by humans in feeding dogs and cats will adversely affect the health of the animal. There are applications at high temperatures at various stages during the production of dog and cat foods. It is predicted that acrylamide (AA) can be formed as a result of a possible Maillard reaction since the cat and dog foods contain starch in its structure. The aim of this study is to determine the AA residue immuno-enzymatically in dry cat and dog feeds from different companies available …

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Investigation Of Antimicrobial Resistance Of Esßl, Amp-C, And Carbapenemaseproducing E. Coli Strains In Retail Poultry Meats, Gülçi̇n Güven, Si̇bel Kizil Jan 2022

Investigation Of Antimicrobial Resistance Of Esßl, Amp-C, And Carbapenemaseproducing E. Coli Strains In Retail Poultry Meats, Gülçi̇n Güven, Si̇bel Kizil

Turkish Journal of Veterinary & Animal Sciences

Antimicrobial agents are used for suppressing some bacterial infections during the rearing of poultry. In this study; the antibiotic resistance properties of Extended Spectrum ßeta-lactamase (ESßL), Amp-C, carbapenemase-producing Escherichia coli (E. coli) strains isolated from chicken breast meat were determined by disc diffusion and E-test methods. Additionally, it is aimed to determine antibiotic resistance genes by classical polymerase chain reaction (PCR). In this context, E. coli strains were isolated from 7 of 100 chicken breast meat (fillets) and identified by a rapid identification system (BBL Crystal). Disc diffusion test results of the isolates were evaluated and 100% to tetracycline; ampicillin, …

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Pathological And Immunohistochemical Changes In The Liver Of Monocrotaline-Treated Rats, Şevket Soylu, Aydin Çevi̇k, Necati̇ Ti̇murkaan Jan 2022

Pathological And Immunohistochemical Changes In The Liver Of Monocrotaline-Treated Rats, Şevket Soylu, Aydin Çevi̇k, Necati̇ Ti̇murkaan

Turkish Journal of Veterinary & Animal Sciences

Pathological and immunohistochemical changes in livers were investigated in monocrotaline-treated rats. Eighty adult-male Wistar rats were distributed in 5 groups, each containing 16 animals according to monocrotaline dose (Group I: 50 mg/kg, Group II: 100 mg/kg, Group III: 150 mg/kg, and Group IV: 200 mg/kg). The control group was administered with physiological saline. Half of the animals in each group were euthanized at the 18th hour while the remaining half at the 6th week postinoculation. At the end of the 18th hour, the most prominent lesion was hepatocytic apoptosis which was dose-dependent. Apoptotic cells were mostly located in periportal and …

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Cryopreservation Of Nili-Ravi Buffalo Bull Semen: Yearly Postthaw Analyses To Depict Climatic Effects, Ejaz Ahmad, Muhammad Shakeel, Zahid Naseer, Adeel Ahmed, Mudussar Nawaz, Muhammad Saleem Akhtar Jan 2022

Cryopreservation Of Nili-Ravi Buffalo Bull Semen: Yearly Postthaw Analyses To Depict Climatic Effects, Ejaz Ahmad, Muhammad Shakeel, Zahid Naseer, Adeel Ahmed, Mudussar Nawaz, Muhammad Saleem Akhtar

Turkish Journal of Veterinary & Animal Sciences

The present study was aimed to observe the postthaw quality of Nili-Ravi buffalo bull semen cryopreserved during heat stress (hot summer months) and normal climatic conditions (fall, winter, and spring months). For this purpose, four (n = 4) adult regular donor Nili-Ravi buffalo bulls were selected to collect and cryopreserve their semen throughout the period of an entire year. The cryopreservation of semen was done following standard freezing protocol. The semen doses cryopreserved during heat stress and normal climatic condition were thawed to determine the sperm motility, motion kinetics, viability, plasma membrane integrity, acrosomal integrity, and morphological abnormalities (head, mid-piece, …

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Plague Risk In The Western United States Over Seven Decades Of Environmental Change, Colin J. Carlson, Sarah N. Bevins, Boris V. Schmid Jan 2022

Plague Risk In The Western United States Over Seven Decades Of Environmental Change, Colin J. Carlson, Sarah N. Bevins, Boris V. Schmid

United States Department of Agriculture Wildlife Services: Staff Publications

After several pandemics over the last two millennia, the wildlife reservoirs of plague (Yersinia pestis) now persist around the world, including in the western United States. Routine surveillance in this region has generated comprehensive records of human cases and animal seroprevalence, creating a unique opportunity to test how plague reservoirs are responding to environmental change. Here, we test whether animal and human data suggest that plague reservoirs and spillover risk have shifted since 1950. To do so, we develop a new method for detecting the impact of climate change on infectious disease distributions, capable of disentangling long-term trends (signal) and …

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Wild Pig Removal Reduces Pathogenic Bacteria In Low-Order Streams, Sara A. Bolds, B. Graeme Lockaby, Latif Kalin, Stephen S. Ditchkoff, Mark D. Smith, Kurt Vercauteren Jan 2022

Wild Pig Removal Reduces Pathogenic Bacteria In Low-Order Streams, Sara A. Bolds, B. Graeme Lockaby, Latif Kalin, Stephen S. Ditchkoff, Mark D. Smith, Kurt Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive wild pig populations have undergone enormous increases in the United States and particularly across the southern U.S. in recent years. High fecundity rates and abilities to adapt quickly to varied habitats have enabled pig populations to become entrenched and difficult to eliminate. The pigs cause many negative impacts on ecosystems including degradation of water quality through infusion of fecal contamination and other non-point source pollutants. Our goal was to determine the effects of pig removal on water quality in streams that were known to be significantly polluted by pig activity Bolds (J Environ Qual 50: 441–453, 2021). We compared …

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A Model For Leveraging Animal Movement To Understand Spatio-Temporal Disease Dynamics, Mark Q. Wilber, Anni Yang, Raoul Boughton, Kezia R. Manlove, Ryan S. Miller, Kim M. Pepin, George Wittemyer Jan 2022

A Model For Leveraging Animal Movement To Understand Spatio-Temporal Disease Dynamics, Mark Q. Wilber, Anni Yang, Raoul Boughton, Kezia R. Manlove, Ryan S. Miller, Kim M. Pepin, George Wittemyer

United States Department of Agriculture Wildlife Services: Staff Publications

The ongoing explosion of fine-resolution movement data in animal systems provides a unique opportunity to empirically quantify spatial, temporal and individual variation in transmission risk and improve our ability to forecast disease outbreaks. However, we lack a generalizable model that can leverage movement data to quantify transmission risk and how it affects pathogen invasion and persistence on heterogeneous landscapes. We developed a flexible model ‘Movement-driven modelling of spatio-temporal infection risk’ (MoveSTIR) that leverages diverse data on animal movement to derive metrics of direct and indirect contact by decomposing transmission into constituent processes of contact formation and duration and pathogen deposition …

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Evaluation Of Ivermectin Antiviral Activity Against Avian Infectious Bronchitis Virus Using A Chicken Embryo Model, Donald L. Reynolds, E. Barry Simpson Jan 2022

Evaluation Of Ivermectin Antiviral Activity Against Avian Infectious Bronchitis Virus Using A Chicken Embryo Model, Donald L. Reynolds, E. Barry Simpson

School of Veterinary and Biomedical Sciences: Faculty Publications

Ivermectin is widely used in both animals and humans as an FDA-approved parasiticide. Ivermectin has also been reported to have antiviral activity against several viruses including coronaviruses. There are reports that indicate ivermectin may have some role in diminishing the disease caused by SARS-CoV-2, but the evidence is inconclusive. The objective of this study was to determine if ivermectin was efficacious in inhibiting avian infectious bronchitis virus (IBV, a coronavirus) replication in chicken embryos. Briefly, our approach was to use the Massachusetts vaccine strain of IBV in combination with various doses of ivermectin and then inoculate these preparations into chicken …

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Rna Nanovaccine Protects Against White Spot Syndrome Virus In Shrimp, Yashdeep Phanse, Supraja Puttamreddy, Duan Loy, Julia Vela Ramirez, Kathleen A. Ross, Ignacio Alvarez-Castro, Mark Mogler, Scott Broderick, Krishna Rajan, Balaji Narasimhan, Lyric C. Bartholomay Jan 2022

Rna Nanovaccine Protects Against White Spot Syndrome Virus In Shrimp, Yashdeep Phanse, Supraja Puttamreddy, Duan Loy, Julia Vela Ramirez, Kathleen A. Ross, Ignacio Alvarez-Castro, Mark Mogler, Scott Broderick, Krishna Rajan, Balaji Narasimhan, Lyric C. Bartholomay

School of Veterinary and Biomedical Sciences: Faculty Publications

In the last 15 years, crustacean fisheries have experienced billions of dollars in economic losses, primarily due to viral diseases caused by such pathogens as white spot syndrome virus (WSSV) in the Pacific white shrimp Litopenaeus vannamei and Asian tiger shrimp Penaeus monodon. To date, no effective measures are available to prevent or control disease outbreaks in these animals, despite their economic importance. Recently, double-stranded RNA-based vaccines have been shown to provide specific and robust protection against WSSV infection in cultured shrimp. However, the limited stability of double-stranded RNA is the most significant hurdle for the field application of these …

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Exo-Sir: An Epidemiological Model To Analyze The Impact Of Exogenous Spread Of Infection, Nirmal Kumar Sivaraman, Manas Gaur, Shivansh Baijal, Sakthi Balan Muthiah, Amit Sheth Jan 2022

Exo-Sir: An Epidemiological Model To Analyze The Impact Of Exogenous Spread Of Infection, Nirmal Kumar Sivaraman, Manas Gaur, Shivansh Baijal, Sakthi Balan Muthiah, Amit Sheth

Publications

Epidemics like Covid-19 and Ebola have impacted people's lives significantly. The impact of mobility of people across the countries or states in the spread of epidemics has been significant. The spread of disease due to factors local to the population under consideration is termed the endogenous spread. The spread due to external factors like migration, mobility, etc. is called the exogenous spread. In this paper, we introduce the Exo-SIR model, an extension of the popular SIR model and a few variants of the model. The novelty in our model is that it captures both the exogenous and endogenous spread of …

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Fertility Control Options For Management Of Free-Roaming Horse Populations, Ursula S. Bechert, John W. Turner Jr., Dan L. Baker, Douglas C. Eckery, Jason E. Bruemmer, Candace C. Lyman, Tulio M. Prado, Sarah R. B. King, Mark A. Fraker Jan 2022

Fertility Control Options For Management Of Free-Roaming Horse Populations, Ursula S. Bechert, John W. Turner Jr., Dan L. Baker, Douglas C. Eckery, Jason E. Bruemmer, Candace C. Lyman, Tulio M. Prado, Sarah R. B. King, Mark A. Fraker

Human–Wildlife Interactions

The management of free-roaming horses (Equus ferus) and burros (E. asinus) in the United States has been referred to as a “wicked problem” because, although there are population control options, societal values will ultimately determine what is acceptable and what is not. In the United States, free-roaming equids are managed by different types of organizations and agencies, and the landscapes that these animals inhabit vary widely in terms of access, size, topography, climate, natural resources, flora, and fauna. This landscape diversity, coupled with contemporary socioeconomic and political environments, means that adaptive management practices are needed to …

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Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah Jan 2022

Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah

Electronic Theses and Dissertations

Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …

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The Effect Of High Dose Ascorbic Acid And High Dcad Administration On Oxidative Stress And Mammary Gland Function Of Dairy Goat Fed Under Tropical Condition, Sapon Semsirmboon Jan 2022

The Effect Of High Dose Ascorbic Acid And High Dcad Administration On Oxidative Stress And Mammary Gland Function Of Dairy Goat Fed Under Tropical Condition, Sapon Semsirmboon

Chulalongkorn University Theses and Dissertations (Chula ETD)

High ambient temperature (HTa) increased the respiration rate (RR) and rectal temperature (Tr) of dairy goats. The excess response resulted in acid-base imbalance, electrolyte imbalance, and systemic oxidative which could indirectly affect the mammary gland. Besides, HTa did directly alter cell function, and this has been proposed to be mediated by oxidative stress. We previously showed that high dietary cation and anion differences (DCAD) could decrease the HTa effect. In dairy cows, a high dose ascorbic acid (Asc) has been used to decrease oxidative stress. Then, It was hypothesized whether mammary gland function could be altered when the HTa was …

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Microencapsulation Of Basil Oil And Its Effect On Replacement Of Antibiotic Growth Promoter, Antioxidant Status And Gut Functions Of Broilers, Sureerat Thuekeaw Jan 2022

Microencapsulation Of Basil Oil And Its Effect On Replacement Of Antibiotic Growth Promoter, Antioxidant Status And Gut Functions Of Broilers, Sureerat Thuekeaw

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this work were 1) to study the preparation and characterization of microencapsulated basil oil (MBO), 2) to determine the stability of MBO against simulated gastrointestinal tract and storage 3) to investigate the antimicrobial and antioxidant properties of MBO and 4) to evaluate the supplementation of MBO on growth performance, antioxidant status, ileal digestibility and intestinal morphology in broiler chickens. The present work demonstrates the use of double-layered polymers (chitosan, CS and sodium alginate, SA) with opposite charges leading to a dual pH responsiveness. The double-layered microcapsule was studied on physicochemical characteristics, the swelling performance as well as …

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Molecular Characterization And Antimicrobial Susceptible Profiles Of Thai Mycoplasma Synoviae And Immune Response Chickens Received Live Mycoplasma Synoviae Vaccine, Kriengwich Limpavithayakul Jan 2022

Molecular Characterization And Antimicrobial Susceptible Profiles Of Thai Mycoplasma Synoviae And Immune Response Chickens Received Live Mycoplasma Synoviae Vaccine, Kriengwich Limpavithayakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mycoplasma synoviae infection in chickens which has been increasing worldwide is mostly controlled by pathogen-free flocks’ maintenance, medication in infected flocks and vaccination in high-risk flocks. The effective control strategy requires the information of diagnostic assays for detecting and differentiating M. synoviae strains, the understanding of immune response mechanisms in vaccinated chickens, and the reliable drug susceptible evidents for making decision in antimicrobial usage. This study aimed to develop a convenient assay for evaluation of quality or uniformity of vaccination with live MS-H vaccine, to characterize the immune response mechanisms in chickens receiving 3 different vaccination programs immunizing single dose …

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Role Of Kca3.1 Channel On Renal Fibrosis In Doxorubicin-Induced Feline Kidney Cell Line, Penpicha Kuedbantakien Jan 2022

Role Of Kca3.1 Channel On Renal Fibrosis In Doxorubicin-Induced Feline Kidney Cell Line, Penpicha Kuedbantakien

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline renal fibrosis is the most common outcome of chronic kidney disease in cats. Nowadays, there is no specific treatment and the mechanism of fibrosis in cats is still unknown. Intermediate conductance Ca2+ activated K+ channel (KCa3.1) plays an important role in novel therapy for organ fibrosis in many species. Transforming growth factor- β (TGF-β)/Smad signaling pathway and KCa3.1 has been shown to relate to renal fibrosis. This study aimed to investigate the relationship between KCa3.1 and feline renal fibrosis via TGF-β/Smad signaling pathway. CRFK cultures were induced with doxorubicin (DOX) to generate feline renal fibrosis model. Triarymethane-34 (TRAM-34), selective …

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Effects Of Microplastics On Gene Expression To Nonspecific Immune System In Pacific White Shrimp (Litopenaeus Vannamei)., Songsak Niemcharoen Jan 2022

Effects Of Microplastics On Gene Expression To Nonspecific Immune System In Pacific White Shrimp (Litopenaeus Vannamei)., Songsak Niemcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Microplastics pollution effects are increasingly observed as new studies emerge in different ecosystem and organismal levels. It is becoming clear that exposure to different types of microplastic can impair the nonspecific immune system of crustaceans. Therefore, this study investigated the impact of high-density polyethylene microplastic particles (HDPE-MP) in feed on nonspecific immune system gene expression of Pacific white shrimp (Litopenaeus vannamei). The LC50 at day 28 of HDPE-MP ingestion was 3.074% w/w of food. Superoxide dismutase (SOD) and the Glutathione peroxidase (GPx) gene were upregulated significantly in groups supplemented with 0.1 and 0.5% of HDPE-MP and then down-regulated significantly after …

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Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott Jan 2022

Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott

Chulalongkorn University Theses and Dissertations (Chula ETD)

PEDV and PDCoV are recognized as important pathogens causing enteric disease in swine herds worldwide. During 2014-2015, several outbreaks of PEDV occurred in many provinces in Thailand, and the clinical signs were more severe than those of classical PEDV in Thailand during 2008-2012. Therefore, the new introduction of PEDV or mutated virus strains was suspected. In 2015, PDCoV was also reported in Thailand, making the situation of enteric disease in the Thai swine industry worse. Since genetic classification studies of PEDV and PDCoV had never been done in Thailand before, this study aimed to investigate the genetic diversity of PEDV …

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Estimating Canine Distemper Virus Exposure In Captive Carnivores In Thai Zoos Using A Pseudotype-Based Serum Neutralization Test, Erngsiri Kaewkhunjob Jan 2022

Estimating Canine Distemper Virus Exposure In Captive Carnivores In Thai Zoos Using A Pseudotype-Based Serum Neutralization Test, Erngsiri Kaewkhunjob

Chulalongkorn University Theses and Dissertations (Chula ETD)

Despite its name, Canine distemper virus (CDV) infection is not restricted to canids but extends to several species of the order Carnivora. The extent of CDV exposure in captive carnivores in Thailand has never been estimated before. Therefore, the objective of this study is to estimate CDV exposure in captive carnivores in Thai zoos using a pseudotype-based neutralization assay (SNT). A total of 264 archived serum samples collected between 2005 and 2020 from captive carnivore species from 6 zoos of the Zoological Park Organization of Thailand (ZPOT) (211 samples) and Tiger Kingdom (TK) (53 samples) were tested in this study. …

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