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2022

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Articles 391 - 416 of 416

Full-Text Articles in Veterinary Medicine

Detection Of Correct Pregnancy Status In Lactating Dairy Cattle Using Mars Data Mining Algorithm, Demet Çanga, Mustafa Boğa Jan 2022

Detection Of Correct Pregnancy Status In Lactating Dairy Cattle Using Mars Data Mining Algorithm, Demet Çanga, Mustafa Boğa

Turkish Journal of Veterinary & Animal Sciences

In this study, it is aimed to determine pregnancy outcomes by using multivariate adaptive regression splines (MARS) algorithm for classification type problems. For this purpose, data obtained from a private dairy farm in the Konya region of Türkiye in 2020 were used to determine pregnancy outcomes in Holstein dairy cattle. It has been determined how to perform statistical analyses on solving classification-type problems with the MARS algorithm and how to use R packages (caret and earth) by creating an R script file. After the analysis, the MARS estimation equation was created and in finding the probability of being pregnant: While …

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Typing Of Rarely Isolated Salmonella Serotypes With Mlst, Seyyi̇de Sariçam İnce, İnci̇ Başak Müştak, Hami̇t Kaan Müştak Jan 2022

Typing Of Rarely Isolated Salmonella Serotypes With Mlst, Seyyi̇de Sariçam İnce, İnci̇ Başak Müştak, Hami̇t Kaan Müştak

Turkish Journal of Veterinary & Animal Sciences

Salmonellosis caused by nontyphoidal Salmonella is the second most common zoonotic infection worldwide. The major foodborne route of this infection is the consumption of contaminated poultry meat and egg. Therefore, monitoring and control of Salmonella agents in poultry populations is critical to reduce the risks for public health. In the poultry industry, the most common serotypes in Salmonella infections are Salmonella enterica subsp. enterica serotype Enteritidis and Typhimurium. In recent years, other frequently isolated Salmonella serotypes have also increased in Turkey because of the Salmonella control programs regulated by many countries. Hence, other Salmonella serotypes (except for the most frequently …

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Pathological And Immunohistochemical Changes In The Liver Of Monocrotaline-Treated Rats, Şevket Soylu, Aydin Çevi̇k, Necati̇ Ti̇murkaan Jan 2022

Pathological And Immunohistochemical Changes In The Liver Of Monocrotaline-Treated Rats, Şevket Soylu, Aydin Çevi̇k, Necati̇ Ti̇murkaan

Turkish Journal of Veterinary & Animal Sciences

Pathological and immunohistochemical changes in livers were investigated in monocrotaline-treated rats. Eighty adult-male Wistar rats were distributed in 5 groups, each containing 16 animals according to monocrotaline dose (Group I: 50 mg/kg, Group II: 100 mg/kg, Group III: 150 mg/kg, and Group IV: 200 mg/kg). The control group was administered with physiological saline. Half of the animals in each group were euthanized at the 18th hour while the remaining half at the 6th week postinoculation. At the end of the 18th hour, the most prominent lesion was hepatocytic apoptosis which was dose-dependent. Apoptotic cells were mostly located in periportal and …

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Determination Of Colony Characteristics And Suitability For Mass Production Of Native Bombus Terrestris L. Population In The Central Black Sea Coast Region, Sali̇m Aktürk, Ayhan Gösteri̇t, Gökhan Akdeni̇z, Samet Okuyan, Ahmet Emi̇r Şahi̇n Jan 2022

Determination Of Colony Characteristics And Suitability For Mass Production Of Native Bombus Terrestris L. Population In The Central Black Sea Coast Region, Sali̇m Aktürk, Ayhan Gösteri̇t, Gökhan Akdeni̇z, Samet Okuyan, Ahmet Emi̇r Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

In this study, two successive generations of queens belonging to a native Bombus terrestris population in the Central Black Sea Region of Türkiye were reared under controlled laboratory conditions to examine its developmental characteristics and suitability for mass production. The first generation was formed by a hundred Bombus terrestris queens which were caught from native fauna in Ordu Province, Türkiye. Young queens and males produced at the end of the life cycle in different colonies of first generation were mated in laboratory conditions. Mated queens were placed in a refrigerated incubator for their two months diapause period. After the diapause …

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Phenotypic And Molecular Characterization Of Streptococcus Agalactiae Causing Bovine Mastitis In Central Region Of Thailand, Sirirat Wataradee Jan 2022

Phenotypic And Molecular Characterization Of Streptococcus Agalactiae Causing Bovine Mastitis In Central Region Of Thailand, Sirirat Wataradee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Streptococcus agalactiae is a highly contagious pathogen with a significant economic impact on bovine mastitis. The research aims to achieve three objectives: 1) identifying and characterizing S. agalactiae strains responsible for bovine mastitis based on their phenotypic and genotypic characteristics, 2) determining susceptibility profiles to antimicrobial agents, and 3) applying molecular epidemiological analysis to explore intramammary infections in Thai dairy herds. We analyzed a collection of one hundred S. agalactiae isolates from clinical and subclinical mastitis cases in thirteen dairy herds in central region of Thailand. We used conventional microbiological procedures, the VITEK® 2 system, and molecular detection of the …

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Influence Of Cataract On Electroretinal Responses In Small Dog Breeds, Chayada Noosen Jan 2022

Influence Of Cataract On Electroretinal Responses In Small Dog Breeds, Chayada Noosen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine cataract is a condition of lens opacity that if severe, can lead to vision loss. High prevalence of cataract is evident in small dog breeds. To assess retinal function in cataract patients, flash electroretinography is commonly used in practice. This study aimed to compare time- and frequency-domain electroretinal responses between normal and cataract eyes in small dog breeds. Eighty-four eyes from small dog breeds categorized into immature cataract (n=49), mature cataract (n=25) and control (n=10) eyes in this study. Full-field flash intensity series ERG was performed in dogs under scotopic and photopic condition. Data was analyzed and compared among …

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Porcine Semen Microbiota Analysis In Relation To Sperm Quality In Semen Extender Coupled With Gentamicin, Cong Bang Ngo Jan 2022

Porcine Semen Microbiota Analysis In Relation To Sperm Quality In Semen Extender Coupled With Gentamicin, Cong Bang Ngo

Chulalongkorn University Theses and Dissertations (Chula ETD)

The present study was carried out to (i) investigate the association between boar semen microbiota and semen qualities and (ii) determine the relationship between boar bacteria contamination and longevity of boar semen preserved for 3 days in BTS with and without gentamicin. A total of 20 Duroc ejaculates were collected from standard quality boars (n=10) and high-quality ones (n= 10). Besides 3 ml of fresh semen of each ejaculate was subjected to 16S rRNA gene sequencing and bioinformatics including alpha diversity, beta diversity, and taxonomic mapping, each ejaculate was diluted in two conditions of Beltsvile Thawing Solution (BTS) extender: CONTROL: …

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The Effect Of Platelet-Rich Plasma And Hyaluronic Acid Nanogel On Wound Healing In Rat Skin Models, Pirada Virankabutra Jan 2022

The Effect Of Platelet-Rich Plasma And Hyaluronic Acid Nanogel On Wound Healing In Rat Skin Models, Pirada Virankabutra

Chulalongkorn University Theses and Dissertations (Chula ETD)

The study aimed to validate two-step procedure PRP protocol and assess the effect on skin healing process in rats treated with PRP and/or HA nanogel. A total of 46 Wistar rats were enrolled in the study. PRPwas produced and its hematological parameters were examined. Six skin defects were created and topicals (control, PRP, HA, PRP/HA, PRP/HA cream, and cream) were applied to the wounds. The progression of wound reduction was photographed daily, and calcutated to wound area. Skin samples were collected according to sampling timepoints: day 0, 3, 7,10, and 14, and stained for histological grading. The platelet (2457.15 $ …

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The Effect Of High Dose Ascorbic Acid And High Dcad Administration On Oxidative Stress And Mammary Gland Function Of Dairy Goat Fed Under Tropical Condition, Sapon Semsirmboon Jan 2022

The Effect Of High Dose Ascorbic Acid And High Dcad Administration On Oxidative Stress And Mammary Gland Function Of Dairy Goat Fed Under Tropical Condition, Sapon Semsirmboon

Chulalongkorn University Theses and Dissertations (Chula ETD)

High ambient temperature (HTa) increased the respiration rate (RR) and rectal temperature (Tr) of dairy goats. The excess response resulted in acid-base imbalance, electrolyte imbalance, and systemic oxidative which could indirectly affect the mammary gland. Besides, HTa did directly alter cell function, and this has been proposed to be mediated by oxidative stress. We previously showed that high dietary cation and anion differences (DCAD) could decrease the HTa effect. In dairy cows, a high dose ascorbic acid (Asc) has been used to decrease oxidative stress. Then, It was hypothesized whether mammary gland function could be altered when the HTa was …

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Effects Of Fingerroot (Boesenbergia Rotunda) Powder On Egg Performance, And Quality, Egg Yolk Malondialdehyde In Laying Hens, Miftah Nurfarid Jan 2022

Effects Of Fingerroot (Boesenbergia Rotunda) Powder On Egg Performance, And Quality, Egg Yolk Malondialdehyde In Laying Hens, Miftah Nurfarid

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to determine the effect of fingerroot powder supplementation on the performance of laying hens, egg quality, and malondialdehyde in the egg yolk. A total of 192 Hy-Line brown laying hens aged 43 weeks were divided into four treatment groups with 48 hens in each group (8 replicates; 6 birds each). The treatments were the control (basal diet), basal diet supplemented with vitamin E at 250 mg/kg, basal diet plus 20 g fingerroot powder/kg, and basal diet plus 40 g fingerroot powder/kg. Performance parameters were analyzed in the following two experimental period: 1 to 3 week and 4 …

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Microencapsulation Of Basil Oil And Its Effect On Replacement Of Antibiotic Growth Promoter, Antioxidant Status And Gut Functions Of Broilers, Sureerat Thuekeaw Jan 2022

Microencapsulation Of Basil Oil And Its Effect On Replacement Of Antibiotic Growth Promoter, Antioxidant Status And Gut Functions Of Broilers, Sureerat Thuekeaw

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this work were 1) to study the preparation and characterization of microencapsulated basil oil (MBO), 2) to determine the stability of MBO against simulated gastrointestinal tract and storage 3) to investigate the antimicrobial and antioxidant properties of MBO and 4) to evaluate the supplementation of MBO on growth performance, antioxidant status, ileal digestibility and intestinal morphology in broiler chickens. The present work demonstrates the use of double-layered polymers (chitosan, CS and sodium alginate, SA) with opposite charges leading to a dual pH responsiveness. The double-layered microcapsule was studied on physicochemical characteristics, the swelling performance as well as …

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Molecular Characterization And Antimicrobial Susceptible Profiles Of Thai Mycoplasma Synoviae And Immune Response Chickens Received Live Mycoplasma Synoviae Vaccine, Kriengwich Limpavithayakul Jan 2022

Molecular Characterization And Antimicrobial Susceptible Profiles Of Thai Mycoplasma Synoviae And Immune Response Chickens Received Live Mycoplasma Synoviae Vaccine, Kriengwich Limpavithayakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mycoplasma synoviae infection in chickens which has been increasing worldwide is mostly controlled by pathogen-free flocks’ maintenance, medication in infected flocks and vaccination in high-risk flocks. The effective control strategy requires the information of diagnostic assays for detecting and differentiating M. synoviae strains, the understanding of immune response mechanisms in vaccinated chickens, and the reliable drug susceptible evidents for making decision in antimicrobial usage. This study aimed to develop a convenient assay for evaluation of quality or uniformity of vaccination with live MS-H vaccine, to characterize the immune response mechanisms in chickens receiving 3 different vaccination programs immunizing single dose …

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Role Of Kca3.1 Channel On Renal Fibrosis In Doxorubicin-Induced Feline Kidney Cell Line, Penpicha Kuedbantakien Jan 2022

Role Of Kca3.1 Channel On Renal Fibrosis In Doxorubicin-Induced Feline Kidney Cell Line, Penpicha Kuedbantakien

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline renal fibrosis is the most common outcome of chronic kidney disease in cats. Nowadays, there is no specific treatment and the mechanism of fibrosis in cats is still unknown. Intermediate conductance Ca2+ activated K+ channel (KCa3.1) plays an important role in novel therapy for organ fibrosis in many species. Transforming growth factor- β (TGF-β)/Smad signaling pathway and KCa3.1 has been shown to relate to renal fibrosis. This study aimed to investigate the relationship between KCa3.1 and feline renal fibrosis via TGF-β/Smad signaling pathway. CRFK cultures were induced with doxorubicin (DOX) to generate feline renal fibrosis model. Triarymethane-34 (TRAM-34), selective …

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Effects Of Microplastics On Gene Expression To Nonspecific Immune System In Pacific White Shrimp (Litopenaeus Vannamei)., Songsak Niemcharoen Jan 2022

Effects Of Microplastics On Gene Expression To Nonspecific Immune System In Pacific White Shrimp (Litopenaeus Vannamei)., Songsak Niemcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Microplastics pollution effects are increasingly observed as new studies emerge in different ecosystem and organismal levels. It is becoming clear that exposure to different types of microplastic can impair the nonspecific immune system of crustaceans. Therefore, this study investigated the impact of high-density polyethylene microplastic particles (HDPE-MP) in feed on nonspecific immune system gene expression of Pacific white shrimp (Litopenaeus vannamei). The LC50 at day 28 of HDPE-MP ingestion was 3.074% w/w of food. Superoxide dismutase (SOD) and the Glutathione peroxidase (GPx) gene were upregulated significantly in groups supplemented with 0.1 and 0.5% of HDPE-MP and then down-regulated significantly after …

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Estimating Canine Distemper Virus Exposure In Captive Carnivores In Thai Zoos Using A Pseudotype-Based Serum Neutralization Test, Erngsiri Kaewkhunjob Jan 2022

Estimating Canine Distemper Virus Exposure In Captive Carnivores In Thai Zoos Using A Pseudotype-Based Serum Neutralization Test, Erngsiri Kaewkhunjob

Chulalongkorn University Theses and Dissertations (Chula ETD)

Despite its name, Canine distemper virus (CDV) infection is not restricted to canids but extends to several species of the order Carnivora. The extent of CDV exposure in captive carnivores in Thailand has never been estimated before. Therefore, the objective of this study is to estimate CDV exposure in captive carnivores in Thai zoos using a pseudotype-based neutralization assay (SNT). A total of 264 archived serum samples collected between 2005 and 2020 from captive carnivore species from 6 zoos of the Zoological Park Organization of Thailand (ZPOT) (211 samples) and Tiger Kingdom (TK) (53 samples) were tested in this study. …

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Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott Jan 2022

Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott

Chulalongkorn University Theses and Dissertations (Chula ETD)

PEDV and PDCoV are recognized as important pathogens causing enteric disease in swine herds worldwide. During 2014-2015, several outbreaks of PEDV occurred in many provinces in Thailand, and the clinical signs were more severe than those of classical PEDV in Thailand during 2008-2012. Therefore, the new introduction of PEDV or mutated virus strains was suspected. In 2015, PDCoV was also reported in Thailand, making the situation of enteric disease in the Thai swine industry worse. Since genetic classification studies of PEDV and PDCoV had never been done in Thailand before, this study aimed to investigate the genetic diversity of PEDV …

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Situation Analysis And Kap On Antimicrobial Use And Resistance Among Veterinarians And Broiler Poultry Farmers Of Nepal, Manoj Kumar Shahi Jan 2022

Situation Analysis And Kap On Antimicrobial Use And Resistance Among Veterinarians And Broiler Poultry Farmers Of Nepal, Manoj Kumar Shahi

Chulalongkorn University Theses and Dissertations (Chula ETD)

The primary goal of this research was to assess the situation analysis and knowledge, attitude, and practices (KAP) of poultry practitioner veterinarians (PPV) and broiler poultry farmers (BPF) regarding antimicrobial use (AMU) and antimicrobial resistance (AMR) in Nepal. The study area covered approximately 88.1% of Nepal's poultry population. A total of 327 PPV from 56 districts and 500 BPF from 40 districts of seven provinces participated. AMU situation and demographic information were collected and analyzed using descriptive statistics and logistic regression analysis to determine associations between variables. Most PPV were male (85.0%) with a mean age of 31.9±7.8 years. Half …

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Associations Of Feline Paramyxoviruses With Pathological Lesions In Cat Kidneys, Aisyah Nikmatuz Zahro Jan 2022

Associations Of Feline Paramyxoviruses With Pathological Lesions In Cat Kidneys, Aisyah Nikmatuz Zahro

Chulalongkorn University Theses and Dissertations (Chula ETD)

Paramyxoviruses in cats composing of feline paramyxovirus (FPaV), feline morbillivirus genotype 1 (FeMV-1), and feline morbillivirus genotype 2 (FeMV-2). These viruses had been recently suggested to be associated with kidney disease in cats. However, pathological consequence of infection is remained restricted. This study aimed to investigate the association of paramyxovirus infection and pathological consequence in cats’ kidney. Renal fresh tissues were collected from routine necropsy in Department of Pathology, Faculty of Veterinary Science, Then, tissues were histopathologically categorized into normal group and pathologic group. To screen paramyxovirus infection, reverse-transcription polymerase chain reaction (RT-PCR) assays using Res-Mor-Hen pan-primer set and specific …

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Correlation Of The Classical Swine Fever (Csf) Antibody Levels Detected By Serum Neutralizaton And Enzyme-Linked Immunosorbent Assay (Elisa), Chairani Ridha Maghfira Jan 2022

Correlation Of The Classical Swine Fever (Csf) Antibody Levels Detected By Serum Neutralizaton And Enzyme-Linked Immunosorbent Assay (Elisa), Chairani Ridha Maghfira

Chulalongkorn University Theses and Dissertations (Chula ETD)

Classical swine fever is an important viral disease that has a devastating impact on the swine industry. The Enzyme-linked immunosorbent assay (ELISA) provides a simpler and more practical approach to detect classical swine fever virus (CSFV) antibodies compared to the serum neutralization (SN) test. However, antibody responses detected by ELISA cannot directly exhibit a protective level. This study aimed to evaluate the correlation of classical swine fever antibody responses detected by SN assay and commercial ELISA. A total of 522 negative and positive serum samples were tested by the SN and ELISA. Correlation, an agreement between two assays, and comparisons …

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The Infection Dynamics, Co-Infection, Multi-Gene Phylogeny, And Molecular Screening Of Plasmodium Caprae Infection In Goats And Mosquito Species, Hoang Lan Anh Nguyen Jan 2022

The Infection Dynamics, Co-Infection, Multi-Gene Phylogeny, And Molecular Screening Of Plasmodium Caprae Infection In Goats And Mosquito Species, Hoang Lan Anh Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Ungulate malaria parasites, including Plasmodium caprae that infects the domestic goats, have received little attention. There are only few studies about the prevalence and mitochondrial genome analyses. This study, therefore, aimed to investigate the natural infection dynamics of P. caprae and its mosquito vectors. Besides, genome analyses based on nuclear genes of P. caprae and other related haemosporidian parasites were also carried out to obtain an in depth understanding about their evolutionary history and origin. A total of 423 goat blood samples were collected during rainy seasons from 2018 to 2021 to assess the malaria infection status. In addition, 1,019 …

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Establishment Of Transplantation Platform For Delivering Mouse Induced Pluripotent Stem Cell-Derived Insulin-Producing Cells (Mips-Ipcs) For Diabetes Treatment, Hong Thuan Tran Jan 2022

Establishment Of Transplantation Platform For Delivering Mouse Induced Pluripotent Stem Cell-Derived Insulin-Producing Cells (Mips-Ipcs) For Diabetes Treatment, Hong Thuan Tran

Chulalongkorn University Theses and Dissertations (Chula ETD)

Pancreatic beta-cell replacement is recognized for feasible type 1 diabetes (T1D) treatment. However, in post-transplantation, the autoimmune destruction incidentally attacks the activity and survival of beta-cells are reported in animal and human. To address these concerns, the generation of immortalized, biocompatible beta-cells, and the engraftment platform are insightfully investigated. The stepwise chemical process was used for in vitro Insulin-producing cells (IPCs) production from mouse gingival fibroblast-induced pluripotent stem cells (mGF-iPSCs). The real-time qRT-PCR, glucose stimulation C-peptide/Insulin secretion, immunostaining, and visible cell methods were examined during IPC differentiation. The encapsulated-IPC beads were loaded into subcutaneous pocket space via transplantation platform. Completed …

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Anti-Proliferative And Apoptotic Effect Of Cannabinoids On Human Pancreatic Ductal Adenocarcinoma Xenograft In Balb/C Nude Mice Model, Quang Trung Le Jan 2022

Anti-Proliferative And Apoptotic Effect Of Cannabinoids On Human Pancreatic Ductal Adenocarcinoma Xenograft In Balb/C Nude Mice Model, Quang Trung Le

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human pancreatic ductal adenocarcinoma (PDAC) is a highly malignant and lethal tumor of the exocrine pancreas. Cannabinoids extracted from the hemp plant Cannabis sativa have been suggested as a cancer therapy due to their anti-tumor effects on several human tumors. However, the anti-tumor effect of cannabinoids on human PDAC is still understudied. Therefore, the objective of the current study is to study the inhibition of proliferation and the induction of apoptosis potencies of cannabinoids in PDAC xenograft nude mice model. The human PDAC cell line (Capan-2) was subcutaneously injected into twenty-five nude mice. After tumor reached 200 mm3 in volume, …

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In Vitro Inhibitory Effects Of Turmeric Extracts Against Biofilm Formation And Virulence Of Flavobacterium Oreochromis, Sirawich Ngernson Jan 2022

In Vitro Inhibitory Effects Of Turmeric Extracts Against Biofilm Formation And Virulence Of Flavobacterium Oreochromis, Sirawich Ngernson

Chulalongkorn University Theses and Dissertations (Chula ETD)

Flavobacterium oreochromis is an opportunistic bacteria and causative agent of columnaris disease that affects freshwater fish worldwide. To initiate infection, the bacteria are required to attach and form biofilm on the fish's skin. The bacteria cells in the biofilm then produce toxins that can degrade fish connective tissue, resulting in lesions and ulcers. This study aimed to evaluate the efficacy of turmeric (Curcuma longa L.) extract by three organic solvents (hexane, ethanol, and methanol) in biofilm inhibition and modulation of biofilm-associated genes of F. oreochromis. Turmeric extracts by three different organic solvents were tested for their ability to inhibit the …

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Molecular Characterization Of Clinostomum Spp. Infecting Cultured Snakeskin Gourami (Trichopodus Pectoralis) In Thailand, Sk Injamamul Islam Jan 2022

Molecular Characterization Of Clinostomum Spp. Infecting Cultured Snakeskin Gourami (Trichopodus Pectoralis) In Thailand, Sk Injamamul Islam

Chulalongkorn University Theses and Dissertations (Chula ETD)

The digeanean Clinostomum spp. is a significant parasitic pathogen of freshwater fish. It has low host specificity and a wide distribution geographically. Morphological and molecular data analyses have been performed on Clinostomum to assess its diversity. However, the molecular occurrence of Clinostomum spp. in aquaculture in Thailand has yet to be reported. Snakeskin gourami (Trichopodus pectoralis), a freshwater fish cultured in Thailand, has significant economic importance. Herein, the study focuses on describing a digenean Clinostomum in the abdominal cavity of T. pectoralis and reports the prevalence of this parasite from central Thailand. Two hundred and sixty-two 4 to 8- month-old …

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Seroprevalence Of Lawsonia Intracellularis Antibody Among Swine Herds In Thailand, December 2021-March 2022, Thanh Nguyen Che Jan 2022

Seroprevalence Of Lawsonia Intracellularis Antibody Among Swine Herds In Thailand, December 2021-March 2022, Thanh Nguyen Che

Chulalongkorn University Theses and Dissertations (Chula ETD)

The gastrointestinal infectious diseases caused by bacteria have been a major problem in the Thai swine industry. Porcine proliferative enteropathy (PPE), also known as porcine ileitis, is an enteric infectious disease caused by Lawsonia intracellularis. Porcine ileitis has been an economic threat to the global pork production system due to various types of diarrheas. Serological diagnosis can be applied for determining pathogen-antibodies seroprevalence and the timing of L. intracellularis infection when introduced to the herd. A lack of serological data since 2009, as well as recent antibiotic usage restrictions, may have a negative impact on porcine ileitis seroprevalence in Thailand. …

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Transferable R Plasmids In Escherichia Coli Isolated From Meat Ducks, Zulqarnain Baqar Jan 2022

Transferable R Plasmids In Escherichia Coli Isolated From Meat Ducks, Zulqarnain Baqar

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to describe the AMR characteristics and transferable R plasmids in Escherichia coli isolated from meat ducks reared in an open house system. One hundred seventy-seven (n=177) commensal E. coli were previously isolated from duck cloacal swabs. In this study, all were biochemically confirmed and examined for their susceptibilities to 15 antimicrobial agents by broth microdilution method. Transfer of R plasmids was tested by biparental mating method followed by plasmid replicon typing (PBRT) and plasmid multi-locus sequence typing (pMLST). The highest resistance rates were observed for ampicillin (83.0%) and tetracycline (81.9%) while multidrug resistance was common (86.4%). Extended-spectrum …

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