Veterinary Medicine Commons^™

A Parapoxviral Virion Protein Inhibits Nf-Κb Signaling Early In Infection, Sushil Khatiwada, Gustavo A. Delhon, Ponnuraj Nagendraprabhu, Sabal Chaulagain, Shuhong Luo, Diego G. Diel, Eduardo F. Flores, D. L. Rock

School of Veterinary and Biomedical Sciences: Faculty Publications

Poxviruses have evolved unique proteins and mechanisms to counteract the nuclear factor κB (NF-κB) signaling pathway, which is an essential regulatory pathway of host innate immune responses. Here, we describe a NF-κB inhibitory virion protein of orf virus (ORFV), ORFV073, which functions very early in infected cells. Infection with ORFV073 gene deletion virus (OV-IA82Δ073) led to increased accumulation of NF-κB essential modulator (NEMO), marked phosphorylation of IκB kinase (IKK) subunits IKKα and IKKβ, IκBα and NF-κB subunit p65 (NF-κB-p65), and to early nuclear translocation of NF-κB-p65 in virusinfected cells (30 min post infection). Expression of ORFV073 alone was sufficient to …

Developing A 670k Genotyping Array To Tag ~2m Snps Across 24 Horse Breeds, Robert J. Schaefer, Mikkel Schubert, Ernest F. Bailey, Danika L. Bannasch, Eric Barrey, Gila Kahila Bar-Gal, Gottfried Brem, Samantha A. Brooks, Ottmar Distl, Ruedi Fries, Carrie J. Finno, Vinzenz Gerber, Bianca Haase, Vidhya Jagannathan, Ted Kalbfleisch, Tosso Leeb, Gabriella Lindgren, Maria Susana Lopes, Núria Mach, Artur Da Câmara Machado, James N. Macleod, Annette Mccoy, Julia Metzger, Cecilia Penedo, Sagi Polani, Stefan Rieder, Imke Tammen, Jens Tetens, Georg Thaller, Andrea Verini-Supplizi

Gluck Equine Research Center Faculty Publications

Background: To date, genome-scale analyses in the domestic horse have been limited by suboptimal single nucleotide polymorphism (SNP) density and uneven genomic coverage of the current SNP genotyping arrays. The recent availability of whole genome sequences has created the opportunity to develop a next generation, high-density equine SNP array.

Results: Using whole genome sequence from 153 individuals representing 24 distinct breeds collated by the equine genomics community, we cataloged over 23 million de novo discovered genetic variants. Leveraging genotype data from individuals with both whole genome sequence, and genotypes from lower-density, legacy SNP arrays, a subset of ~5 million high-quality, …

Seroepidemiology Of Sarcocystis Neurona And Neospora Hughesi Infections In Domestic Donkeys (Equus Asinus) In Durango, Mexico, Cosme Alvarado-Esquivel, Daniel K. Howe, Michelle R. Yeargan, Domingo Alvarado-Esquivel, José Alfredo Zamarripa-Barboza, Jitender P. Dubey

Maxwell H. Gluck Equine Research Center Faculty Publications

There is currently no information regarding Sarcocystis neurona and Neospora hughesi infections in donkeys in Mexico. Here, we determined the presence of antibodies against S. neurona and N. hughesi in donkeys in the northern Mexican state of Durango. Serum samples of 239 domestic donkeys (Equus asinus) were assayed for S. neurona and N. hughesi antibodies using home-made enzyme-linked immunoassays; six (2.5%) of the 239 donkeys tested seropositive for S. neurona. The seroprevalence of S. neurona infection was comparable among donkeys regardless of their origin, health status, or sex. Multivariate analysis showed that seropositivity to S. neurona was …

Absence Of Typical Haversian System From The Compact Bone Of Some Reptile And Bird Species, Yasser Ahmed, Mohamed Abdelsabourv Khalaf, Fatma Khalil

Histology Collection

Background and Objective: Mammalian compact bone is composed mostly of Haversian system. Although there are many studies describing the typical Haversian system in mammals, there are few studies conducted on bones from non-mammalian species. The objective of the current study was to investigate the existence of the typical Haversian system in compact bones from reptiles and birds.

Materials and Methods: Femora were collected from geckos, Nile monitors, sparrows, ducks and geese. Samples were then, fixed in 10% paraformaldehyde and embedded in paraffin. Paraffin sections were stained with hematoxylin and eosin or Von Kossa and then examined using light microscopy.

Results: …

Cross Reactivity Of Immune Responses To Porcine Reproductive And Respiratory Syndrome Virus Infection, Ignacio Correas, Fernando A. Osorio, David Steffen, Asit K. Pattnaik, Hiep L. X. Vu

School of Veterinary and Biomedical Sciences: Faculty Publications

Because porcine reproductive and respiratory syndrome virus (PRRSV) exhibits extensive genetic variation among field isolates, characterizing the extent of cross reactivity of immune responses, and most importantly cell-mediated immunity (CMI), could help in the development of broadly cross-protective vaccines. We infected 12 PRRSV-naïve pigs with PRRSV strain FL12 and determined the number of interferon (IFN)-γ secreting cells (SC) by ELISpot assay using ten type 2 and one type 1 PRRSV isolates as recall antigens. The number of IFN-γ SC was extremely variable among animals, and with exceptions, late to appear. Cross reactivity of IFN-γ SC among type 2 isolates was …

Growing South Dakota (Summer 2017), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the 2016 SDSU Extension Annual Report.

Page] 2 From the Director
[Page] 3 WaterTouches Everything, Multi-State Network Strives to Enhance Water Stewardship
[Page] 6 From RubbleTo Renewal, Unique Art Project Helps Delmont Community Heal
[Page] 7 Future Focused, Community Development Program Helps Harness Ideas Into Action
[Page] 8 Health Help, Statewide Program Strives To Help Individuals Better Manage Chronic Health Condition
[Page] 10 Making An Impact, Master Gardener Program Helps Individuals & Communities Flourish
[Page] 12 Statewide Highlights & Happenings
[Page] 14 Outreach to Youth, Programs Foster Engagement With Diverse Youth Audiences
[Page] 17 A Look At …

Equine Arteritis Virus Has Specific Tropism For Stromal Cells And Cd8+ T And Cd21+ B Lymphocytes But Not For Glandular Epithelium At The Primary Site Of Persistent Infection In The Stallion Reproductive Tract, Mariano Carossino, Alan T. Loynachan, Igor F. Canisso, Richard Frank Cook, Juliana Roberta Campos, Bora Nam, Yun Young Go, Edward L. Squires, Mats H. T. Troedsson, Thomas W. Swerczek, Fabio Del Piero, Ernest F. Bailey, Peter J. Timoney, Udeni B. R. Balasuriya

Veterinary Science Faculty Publications

Equine arteritis virus (EAV) has a global impact on the equine industry as the causative agent of equine viral arteritis (EVA), a respiratory, systemic, and reproductive disease of equids. A distinctive feature of EAV infection is that it establishes long-term persistent infection in 10 to 70% of infected stallions (carriers). In these stallions, EAV is detectable only in the reproductive tract, and viral persistence occurs despite the presence of high serum neutralizing antibody titers. Carrier stallions constitute the natural reservoir of the virus as they continuously shed EAV in their semen. Although the accessory sex glands have been implicated as …

No Lasix Racing: Good Idea Or Failed Experiment?, Clara Fenger, Thomas Tobin, Peter Ecabert

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.

Outcomes Of Spatially Fractionated Radiotherapy (Grid) For Bulky Soft Tissue Sarcomas In A Large Animal Model, Michael W. Nolan, Tracy L. Gieger, Alexander A. Karakashian, Mariana N. Nikolova‑Karakashian, Lysa P. Posner, Donald M. Roback, Judith N. Rivera, Sha Chang

Physiology Faculty Publications

GRID directs alternating regions of high- and low-dose radiation at tumors. A large animal model mimicking the geometries of human treatments is needed to complement existing rodent systems (eg, microbeam) and clarify the physical and biological attributes of GRID. A pilot study was undertaken in pet dogs with spontaneous soft tissue sarcomas to characterize responses to GRID. Subjects were treated with either 20 Gy (3 dogs) or 25 Gy (3 dogs), delivered using 6 MV X-rays and a commercial GRID collimator. Acute toxicity and tumor responses were assessed 2, 4, and 6 weeks later. Acute Radiation Therapy Oncology Group grade …

New Methods To Differentiate Cardiac And Non-Cardiac Disease Cats From Thoracic Radiographs, Pakapol Fucharoen, Wannisa Pankham, Siwakorn Thong-In, Sirapop Juengthanasomboon, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

The objective of this study was to distinguish between cats with cardiac disease and non-cardiac disease by using parameters obtained from thoracic radiographs. This study was performed in 49 cats with a mean age of 7.7±4.79 years. Weight, sex and breed of all cats were randomly chosen. Echocardiography was used as a gold standard to diagnose cardiac disease and separate the cats into two groups, cardiac disease (n=31) and non-cardiac disease (n=18). Five parameters were measured from the right lateral radiographs including 1) the cardiac length (L), 2) the cardiac width (W), 3) the width of the caudal vena cava …

Effects Of Cryopreservation And Leukodepletion On Morphological Index Of Red Blood Cells In Dogs, Deniz Aktaran Bala, Ibrahim Akyazi

The Thai Journal of Veterinary Medicine

Preservation of blood components for transfusion is a long-standing phenomenon. However, studies of cryopreservation of leukodepleted canine erythrocytes are quite limited and no studies are available with respect to morphological evaluation of erythrocytes during the cryopreservation process. The aim of this study was to determine morphological changes caused by cryopreservation, storage and leukocyte filtration. Healthy adult dogs meeting the criteria for blood transfusion were used in this study. Packed red blood cells (pRBCs) were obtained from each dog. The samples were divided into two groups. Leukocyte filtration was performed on one group of pRBCs. The other group received no filtration. …

Surgical Treatment Of Spontaneous Pneumothorax Associated With Lobal Emphysema In A Dog, Yawon Hwang, Seungwon Yoo, Gonhyung Kim

The Thai Journal of Veterinary Medicine

A 3-year-old female Maltese dog was admitted with cough, dyspnea, and gasping. There was no sign of trauma in history taking. It was speculated that pneumothorax occurred in the right pleural space after X-ray examination. Thoracocentesis and chest tube were applied for 5 days. The symptoms of dyspnea and cough were, however, not relieved or recurred. Exploratory thoracotomy was considered to be the most appropriate approach. Spongiform and air-containing tissue was identified in the whole parenchyma in the right middle lobe. Total lobectomy was performed and then a chest tube was inserted. Atrophy of the right cranial lobe and collapse …

Expression Of Mir-29a And Ifn-Γ In Pigs Vaccinated With Modified Live Prrs Vaccine, Sunaree Nantakhruea, Nuntawan Aunjangwang, Orawan Limsivilai, Win Surachetpong

The Thai Journal of Veterinary Medicine

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the important RNA viruses that affects swine production worldwide. To better improve vaccine efficacy, understanding the molecular mechanisms and viral-host interaction is important to increase the knowledge of vaccine response. The objective of this study was to investigate the expression levels of miR-29a and IFN-γ in pigs that received modified live PRRSV vaccine. The study revealed that both miR-29a and IFN-γ transcript levels increased in vivo early, at day 3 post vaccination, whereas levels of miR-29a and IFN-γ mRNA were not different at day 7 post vaccination. Our study is …

Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram

The Thai Journal of Veterinary Medicine

-

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

-

Determination Of Age And Construction Of Population Age Structure Of Wild Asian Elephants Based On Dung Bolus Circumference, Chalita Kongrit, Chomcheun Siripunkaw

The Thai Journal of Veterinary Medicine

Estimating the age of wildlife is an important technique for the construction of a population age structure that could be useful for the prediction of population change. The age of wild elephants can be reliably estimated from the size of dung bolus circumference which correlates with elephant growth. This research aimed to determine a cut-off bolus circumference for the mature age class of wild Asian elephants at Salakphra Wildlife Sanctuary as inferred from the social behavior of male elephants. Males living within their natal groups were considered immature males, whereas solitary males were considered mature males. The largest bolus circumference …

Comparative In Vitro Killing Activities Of Enrofloxacin When Used Alone And In Combination With Doxycycline Against E. Coli Isolates From Dogs And Cats, Supapatt Kireewan, Nipattra Suanpairintr

The Thai Journal of Veterinary Medicine

Enrofloxacin (ENR) exhibits bactericidal activity while doxycycline (DOX), a drug of choice for Ehrlichia canis (E. canis), demonstrates bacteriostatic effect. When Gram-negative bacteria and E. canis co-infection occurs, using enrofloxacin concurrently with doxycycline is inevitable. The objectives of this study were to compare in vitro killing activities and killing times of enrofloxacin when used alone with those of its combination with doxycycline against ten Escherichia coli (E. coli) isolates from dogs and cats using time-kill curves. The combination treatment was administered either simultaneously (ENR+DOX at t=0 hr) or separately (DOX at t=0 followed by ENR at t=12 hr). The best …

Evaluation Of Antimicrobial Peptide K9cath In A Murine Model Of Mastitis, Alberto Barreras-Serrano, Alma Rossana Tamayo-Sosa, Víctor Manuel Del Villar-Pérez, Alma Angelina Castellanos-Félix, Gilberto López-Valencia, Luis Tinoco-Gracia, Rogelio Hernández-Pando, Tonatiuh Melgarejo

The Thai Journal of Veterinary Medicine

In the present study the effect of antimicrobial peptide K9CATH was evaluated in a murine model of mastitis by Staphylococcus aureus. Mastitis was induced with a clinical isolate of S. aureus in 20 female Balb/c mice at 7 to 10 days of lactation. Ten mice (treatment group) received daily intramammary infusions of 32 μg/50 μL K9CATH peptide, for 3 consecutive days, and 10 other mice (control group) received only 50 μL of saline solution. Results showed a significant reduction (P<0.01) in S. aureus CFU counts in the treatment group compared to the control group. These data suggest that the K9CATH peptide could be a potential candidate for the treatment of bovine mastitis.

Study Of Testicular Immunolocalization Of Inhibin Subunits And Epididymal Histological Structures Among Different Antler Status In Rusa Deer (Rusa Timorensis), Apichaya Sudsukh, Kazuyoshi Taya, Gen Watanabe, Kentaro Nagaoka, Worawidh Wajjwalku, Nikorn Thongtip

The Thai Journal of Veterinary Medicine

The number of rusa deer (Rusa timorensis) raising farms has highly increased in Thailand. Basic data on the reproduction of rusa deer are essential for improvement in deer farming reproduction and the assisted reproductive technologies (ARTs) of endangered deer species. This study aimed to determine the relation of antler changes to testicular and epididymis histological structure and cellular source of testicular inhibin subunits in rusa deer. Eighteen rusa deer testes and epididymis were collected from a slaughterhouse located in Kamphaeng Saen, Nakhon Pathom, Thailand. The male rusa deer used in this study were separated into three groups depending on antler …

In Vivo Bone Regeneration Of Thai Silk Fibroin Scaffolds With Gelatin, Hydroxyapatite And Hyaluronic Acid, Tonporn Lamlerd, Kumpanart Soontornvipart, Sawang Kesdangsakonwut, Sorada Kanokpanont, Siriporn Damrongsakkul

The Thai Journal of Veterinary Medicine

Three-dimensional Thai silk fibroin-based scaffolds have been developed and reported in our previous study on their appropriate morphology, physical properties and, in particular, promising potential to promote the growth of bone cells in vitro. Moreover, our previous study found that they were non-toxic to cells in vivo. In this work, three types of Thai silk fibroin-based scaffolds, including conjugated gelatin/Thai silk fibroin scaffold (CGSF), hydroxyapatite/conjugated gelatin/Thai silk fibroin scaffold (CGSF4) and hyaluronic acid/Thai silk fibroin scaffold (HSF), were investigated for their in vivo osteogenic potential in rat model. Each Thai silk fibroin-based scaffold was implanted in the bone defect (6 …

Effects Of Proportion Of Cassava And Lactic Acid-Treated Cassava In Rations On Rumen Ph And Plasma Lipopolysaccharide-Binding Protein In Beef Cattle, Rittichai Pilachai, Weerachai Thongdee, Yauvapol Chumpol, Suvaluk Seesupa, Jan Thomas Schonewille

The Thai Journal of Veterinary Medicine

The objectives of this study were to investigate the effect of the proportion of cassava and lactic acid (LA)-treated cassava in rations on rumen fermentation parameters and plasma lipopolysaccharide-binding protein (LBP) in beef cattle. Four, rumen cannulated, beef cattle were randomly assigned to four experimental rations in a study with a 2 × 2 factorial arrangement in a 4 × 4 Latin square with a 16-day adaptation period, followed by a 5-day experimental period. During the 5 days of experimental period, the cattle were subjected to 4 experimental treatments, i.e. rations that contained either a high proportion or a low …

Streptococcus Agalactiae Serotype Ib, An Emerging Pathogen Affecting Climbing Perch (Anabas Testudineus) And Günther’S Walking Catfish (Clarias Macrocephalus) Polycultured In Southern Thailand, Chutima Klingklib, Naraid Suanyuk

The Thai Journal of Veterinary Medicine

Streptococcus agalactiae (Group B Streptococcus; GBS) causes serious damage to fish aquaculture worldwide. The present study reports S. agalactiae infection in climbing perch (Anabas testudineus) and Günther’s walking catfish (Clarias macrocephalus) polycultured in southern Thailand between 2011 and 2015. During the disease outbreak, a mortality rate of 10-40% was observed, affecting climbing perch and Günther’s walking catfish weighing from 60-150 g and 30-90 g, respectively. The infected fish exhibited a variety of symptoms typical of streptococcosis, including lethargy, exophthalmia, corneal opacity, ascites, haemorrhage and erratic swimming. One hundred and twenty six isolates from the infected fish were identified as S. …

Seroprevalence And Molecular Investigation Of Brucellosis In Camels Of Selected Districts Of Punjab, Pakistan, Asim Shahzad, Ahrar Khan, Muhammad Zargham Khan, Muhammad Saqib

The Thai Journal of Veterinary Medicine

Camels still play an important role in providing food security in several developing countries, especially in the arid zones. As brucellosis has zoonotic importance, thereby diseased camels can infect humans chiefly via milk. In spite of its vital importance, limited studies are available of brucellosis in camels in Pakistan. Keeping in view the fact, this study was designed and executed. For this purpose, serum samples (n=761) from various herds of camel with history of abortion from different locations of Punjab province, Pakistan were collected. Initial screening was done using Rose Bengal plate test (RBPT). To avoid improper judgment of the …

Seroprevalence Of Bluetongue Virus Infection And Associated Risk Factors In Domestic Ruminants In The South Of Iran, Mohsen Manavian, Majid Hashemi, Davood Nikoo, Farhang Tavan, Seyed Mohammad Hossein Hosseini, Mehran Bakhshesh, Mohammad Hossein Marhamatizade

The Thai Journal of Veterinary Medicine

A cross-sectional study was designed and 3,872 blood samples were collected from apparently healthy sheep, goats and cows in Fars province, the south of Iran. The sera were screened for detection of bluetongue virus (BTV) antibodies using a commercially competitive enzyme-linked immunosorbent assay kit. Overall prevalence rate of BTV antibodies among the domestic ruminants was 57.64% (70.93%, 55.70% and 19.77% for sheep, goat and cow, respectively). Associations between each independent variable, including ruminant type, age, sex, region, farming system and breed, and BTV infection were statistically significant (p<0.05). Females were 1.32 times more likely to exhibit seropositivity. Odds ratio for BTV infection was higher in older animals and traditional farming. The results revealed a wide spread of BTV infection in the domestic ruminants, especially in sheep and goat. Further studies of the distribution of Culicoides vectors in the region, virus isolation and genome sequencing of the isolated viruses are suggested.

Effects Of Chikungunya Virus Titers In Blood Meals On Virus Infection, Dissemination, And Transmission In Asian Tiger Mosquito: Aedes Albopictus (Diptera: Culicidae), Ranida Tuanudom, Nichapat Yurayart, Sonthaya Tiawsirisup

The Thai Journal of Veterinary Medicine

Chikungunya virus (CHIKV) is an important mosquito-borne virus and transmission cycle of this virus involves mosquito vectors and infected vertebrate hosts. However, the study about vector competence for CHIKV in Thailand is limited. This study was conducted to examine the effects of CHIKV titers in blood meals on vector competence of Aedes albopictus (Diptera: Culicidae). Five groups of Ae. albopictus were allowed to feed on different levels of CHIKV in the blood meals which were 102, 103, 104, 105, and 106 CID50/ml of CHIKV. Body, legs and wings, and saliva samples from blood-fed mosquitoes were assayed for the presence of …

Development Of Indirect Enzyme-Linked Immunosorbent Assay For Antibody Detection Against Avibacterium Paragallinarum, Panchita Hongprasertkul, Wisanu Wanasawaeng, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Indirect enzyme-linked immunosorbent assay (I-ELISA) for antibody detection against A. paragallinarum serovars A, B and C was developed in separated plates. One hundred Babcock 308 female layer chickens were randomly divided into five groups of 20 each. Groups 1, 2, 3 and 4 were different positive control groups of immunized chickens with commercial trivalent mineral oil vaccine, and prepared bacterins of Avibacterium paragallinarum serovars A (221), B (0222) and C (Modesto), respectively. The chickens in Group 5 were assigned as a negative control. Positive and negative control sera from the chickens in Groups 1-5 at 2 weeks after the second …

Effect Of A Prebiotic, Probiotic And Symbiotic On Performance Of Broilers Under Clostridium Perfringens Challenge, Ahmed Abdulaziz Al-Sagan, Alaeldein Mahmoud Abudabos

The Thai Journal of Veterinary Medicine

The risk of developing antibiotic-resistant pathogenic bacteria is a major concern in poultry production. The aim of this paper was to evaluate a number of commercially available products as alternatives to antimicrobial growth promoters (AGPs) in broiler’s feed. Six experimental treatments [T1 = unmediated feed (negative control, NC); T2 = unmediated feed plus bacterial challenge (positive control, PC); T3 = feed plus antibiotic (NEOX); T4 = feed plus probiotic (GALI); T5 = feed plus prebiotic (TECHNO); and T6 = feed plus probiotic and prebiotic (SYM)] were used in this study. A total of 240 one-day-old chicks were utilized in the …

Temporohyoid Osteoarthropathy In A Mare With Unilateral Facial Paralysis And Vestibular Signs, Hamid Tavanaeimanesh, Majid Masoudifard, Seyyed Hosein Moaddab

The Thai Journal of Veterinary Medicine

The aim of this case report was to present clinical signs and radiographic features of one of the most important peripheral vestibular diseases in horse, and also present the treatment for this disease. Temporohyoid osteoarthropathy (THO) is caused by proliferation of the proximal stylohyoid bone and petrous temporal bone which finally results in ankylosis of the temporohyoid joint. Any sudden movement may result in damage to the joint and also structures in its vicinity. Some peripheral nerves especially cranial nerve VII and VIII may be affected and THO manifestation appears. The mare presented in this report was referred with unilateral …

What Is Your Diagnosis?, Chutimon Thanaboonnipat, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

-

Endocrine And Metabolic Effects Of Consuming Sugar-Sweetened Beverages: Preclinical And Clinical Studies", Peter Havel

Science Seminar Series

Peter J. Havel of the School of Veterinary Medicine at UC Davis will speak on his research on Endocrine and Metabolic Effects of Consuming Sugar-Sweetened Beverages: Preclinical and clinical studies at this Science Seminar Series lecture.