Veterinary Medicine Commons^™

Serotypes Of Salmonella Isolated From Feces Of Cattle, Buffalo,And Camel And Sensitivities To Antibiotics In Turkey, Hasan Hüseyi̇n Hadi̇mli̇, Yasemi̇n Pinarkara, Asli Sakmanoğlu, Zafer Sayin, Osman Ergani̇ş, Ali̇ Uslu, Huda Jihad Al-Shattrawi

Turkish Journal of Veterinary & Animal Sciences

In this study, Salmonella strains from dairy cows, calves with diarrhea, and buffalo and camel feces were isolated and serotyped. There were 869 feces samples (437 calves, 287 dairy cows, 100 buffalo, and 45 camels) collected from 13 provinces and 21 farms. Preenrichment feces samples were added to Rappaport-Vassiliadis medium. Samples were then subcultured on xylose lysine tergitol-4 agar and plates. Suspected colonies of Salmonella were confirmed by latex agglutination test. The isolates of Salmonella spp. were serotyped at the Etlik Central Veterinary Control Institute. In total, 40 Salmonella spp. were isolated, including 33 from calves, 5 from dairy cows, …

Comparative Investigation Of Some Behavior Traits Of Honamlı,Hair, And Saanen Goats In A Mediterranean Maquis Area, Aykut Asim Akbaş, Özkan Elmaz, Mustafa Saatci

Turkish Journal of Veterinary & Animal Sciences

The study was conducted to investigate comparatively some behavior traits (grazing, resting, bipedal stance, agonistic behavior, rumination, and browsing) of Honamlı, Hair, and Saanen goat breeds in the morning and afternoon periods between April and September in maquis shrublands. A total of 30 two-year-old goats including 10 from each goat breed were used. While grazing behavior of Saanen goats was observed mostly in April and May as 75.45%-71.65% and 73.16%-78.25% for morning and afternoon in these months, respectively, these values were respectively 62.52%-64.23% and 59.94%-67.67% for Hair goats and 60.12%-62.94% and 46.42%-54.78% for Honamlı goats. It was observed that Hair …

Comparison Of The Isolation And Inhibition Abilities Ofselective Media Used For Brucella Spp. Isolation, Mustafa Sencer Karagül, Serkan İki̇z

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare and contrast the isolation ability of selective media developed for Brucella spp. isolation and their inhibition ability against contaminant microorganisms. Fifty-one field strains biotyped from abortion case samples and 25 Brucella spp. negative organ samples were used. Strain suspensions and organ suspensions were prepared separately. The turbidity of the strain suspensions was measured via spectrophotometer. The bacterial density of the strain suspensions was prepared in such a way that the suspensions would have different turbidity values. Inoculation into Farrell, Agrifood Research and Technology Center of Aragon, Jones & Morgan, and Modified Thayer …

Ryegrass Pasture Supplementation Strategies For Lactating Ewe Performance, Sthefany Santos, Margarete Falbo, Itacir Sandini, Larissa Ishiy, Andressa Deliberalli, Leticia Posso

Turkish Journal of Veterinary & Animal Sciences

Optimal ewe health and well-being favors good reproductive performance and, as a consequence, assures good growth of the offspring. Thus, the aim of this study was to evaluate three production systems using commercial concentrate supplementation on Italian ryegrass pasture (Lolium multiflorum) on the metabolic profile and body condition score (BCS) of ewes, from those recently postpartum to 75 days in lactation. The total lamb production per ewe was also evaluated. Eighteen adult twin-bearing ewes, Texel x Ile de France crossbreeds, and their offspring were divided into three groups: without supplementation - control (CON); supplemented ewes and lambs at 1% of …

The Csnp Scanning And Expression Analysis Of The Duck Fth1 Gene, Yu Zhang, Yiyu Tong, Yang Chen, Zhengyang Huang, Zhen Zhu, Yang Zhang, Qi Xu, Guohong Chen

Turkish Journal of Veterinary & Animal Sciences

Ferritin, heavy polypeptide 1 (FTH1) is a kind of ferritin complex that catalyzes the conversion of Fe(II) into Fe(III) to protect the cell from oxidative damage. Recent research has shown that FTH1 is also associated with several disease-related processes, such as inflammation and tumor progression and so on. In this study, the coding sequence (CDS) of FTH1 in Jinding ducks was cloned. The CDS sequence revealed an open reading frame of 546 nucleotides encoding a protein with 181 amino acids. Based on this sequence, the duck FTH1 protein is predicted to have conserved domains typical of eukaryotic ferritin. Only one …

Effect Of Different Processing Methods Of Pigeon Pea (Cajanus Cajan) On Growth Performance, Carcass Traits, And Blood Biochemical And Hematological Parametersof Broiler Chickens, Muhammad Arif, Abdur Rehman, Muhammad Saeed, Mohamed Ezzat Abd El-Hack, Mahmoud Alagawany, Haider Abbas, Muhammad Asif Arian, Sarfaraz Ali Fazlani, Abbasi Imtiaz Hussain, Tugay Ayaşan

Turkish Journal of Veterinary & Animal Sciences

A trial was conducted to evaluate the effect of different processing methods of pigeon pea (Pp; Cajanus cajan) on fattening performance and carcass traits as well as blood biochemical and hematological parameters of broiler chickens. For this purpose, 300 day-old chicks were assigned to 5 treatments. Each treatment had 5 replicates and contained 12 birds each under a completely randomized design. Five isonitrogenous and isocaloric diets were formulated with the inclusion of pigeon pea crushed (control, PPC), boiled with potash (PPP), boiled (PPB), soaked (PPS), or roasted (PPR) at the rate of 20% of the basal diet. The highest values …

Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning

Turkish Journal of Veterinary & Animal Sciences

Tick-borne diseases are one of the most important classes of disease in animal husbandry and cause severe economic losses. In this study, 660 adult female ticks were collected from sheep in nine localities in Henan Province, China. All were identified as Haemaphysalis longicornis, confirmed with light microscopy and PCR amplification. The pathogens identified in these ticks included bacteria of the genus Anaplasma (Anaplasma ovis, A. bovis, A. phagocytophilum) and piroplasmal protozoans (Theileria luwenshuni, Babesia motasi). Our results show the high prevalence of A. bovis (20.4%, 135/660) and T. luwenshuni (14.4%, 95/660) in the ticks from sheep, whereas A. ovis, A. …

Antimicrobial Resistance Profiles And Virulence Factors Of Escherichia Colio157 Collected From A Poultry Processing Plant, Harun Hizlisoy, Serhat Al, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan, Kadi̇r Semi̇h Gümüşsoy

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were to investigate the presence of Escherichia coli O157 and to determine the antibiotic susceptibility of isolates in different poultry-originated materials. In total 300 samples of chicken carcass swabs (n = 50), chicken feces (n = 50), gizzard (n = 50), liver (n = 50), water in the final scald tank (n = 50), and equipment such as tables, conveyers, and knives (n = 50) were used as materials. Overall, 45 E. coli isolates were obtained from the 300 poultry samples. However, 13 (4.3%) of the samples were identified as E. coli O157 including 3 …

Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

The present study was undertaken to investigate the immunohistochemical expression of pulmonary surfactant proteins (SP-A, SP-B, proSP-C) associated with proliferating cell nuclear antigen (PCNA), thyroid transcription factor-I (TTF-I), and lymphocytic phenotypes (CD3+ T and CD79αcy+ B cells) in ovine lungs with exudative and verminous pneumonia. Pneumonic lungs (n = 50) were classified as presenting fibrinous bronchopneumonia (n = 16), abscessing necrotic bronchopneumonia (n = 10), verminous pneumonia (n = 15), and interstitial pneumonia (n = 9). The lungs with fibrinous bronchopneumonia displayed consolidation of lobes with adhesion to surrounding tissues. The lungs with verminous pneumonia were characterized by lungworm sections …

A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi

Turkish Journal of Veterinary & Animal Sciences

Ticks are the main hematophagous arthropods and obligatory ectoparasites that are considered a vector of serious pathogens for animals and humans. The aims of the present survey were to assess the prevalence and diversity of ticks in cattle in Southeast Iran and to determine Coxiella burnetii in the ticks. From May 2014 to April 2015, 583 ticks were collected randomly from 257 cattle. Nested trans-PCR was performed to detect C. burnetii. The overall prevalence of hard tick infestation on cattle was 56.8%. In total, seven different species were identified: Rhipicephalus sanguineus sensu lato (32.6%), Hyalomma anatolicum (28.8%), Hyalomma excavatum (19%), …

Impact Of Different Housing Systems And Age Of Layers On Egg Quality Characteristics, Bi̇lgehan Yilmaz Di̇kmen, Aydin İpek, Ümran Şahan, Arda Sözcü, Süleyman Can Baycan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate effects of conventional-cage (CC), enriched-cage (EC), and free-range (FR) systems and hen age on internal and external egg quality parameters of layers (Lohmann Brown). A total of 720 eggs were analyzed for egg weight (EW), shell weight (SW), yolk weight (YW), albumen weight (AW), shell thickness (ST), shell breaking strength (SBS), shape (SI), albumen (AI), yolk index (YI) of eggs, shell ratio of eggs (SR), albumen ratio of eggs (AR), yolk ratio of eggs (YR), yolk color (YC), and Haugh unit (HU). The highest EW, YW, AW, SW, AI, YI, HU (all …

Radiographic Examination Of Hip Joint Geometry In Healthy Sivas Kangal Dogs, Murat Sarierler, Zeynep Bozkan Tatli, İbrahi̇m Akin, Rahi̇me Yaygingül, Onur Özgün Deri̇ncegöz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the normal ranges of proximal femoral and acetabular parameters in 26 adult healthy Sivas Kangal dogs (SKDs). Significant differences were observed between the sexes in hip axis length (HAL) of 8.03 ± 0.82 cm and 7.47 ± 0.40 cm, femoral neck axis length-a (FNALa) of 6.40 ± 0.55 cm and 6.09 ± 0.27 cm, FNALb of 5.09 ± 0.47 cm and 4.78 ± 0.31 cm, acetabular width (AW) of 1.62 ± 0.35 cm and 1.37 ± 0.24 cm, femoral shaft diameter (FSD) of 3.11 ± 0.31 cm and 2.88 ± 0.21 cm, …

Investigation Of Effects Of Fucoidan Polysaccharides Extracted From Twospecies Of Padina On The Wound-Healing Process In The Rat, Moazameh Kordjazi, Bahareh Shabanpour, Ebrahim Zabihi, Mohammad Ali Faramarzi, Hassan Ahmadi Gavlighi, Seyed Mohammad Amin Feghhi, Seyed Abbas Hosseini

Turkish Journal of Veterinary & Animal Sciences

Seaweeds contains different types of polysaccharides with high abilities. The aim of this study was to determine the potential of fucoidan polysaccharides for wound-healing properties. Fucoidans of two species of Padina (P. tetrastromatica and P. boergesenii), collected from the Persian Gulf northern coastal area, were extracted and analyzed using high-performance anion-exchange chromatography. Purified fucoidans were used to prepare a 2% topical ointment. Under aseptic conditions, two identical paravertebral full-thickness skin wounds (either by burn or excision) were made in 40 anesthetized male rats allocated into 4 groups. Either fucoidan ointments or the vehicle (ointment base) were applied to rats' wounds …

Effects Of Nigella Sativa And Hypericum Perforatum On Wound Healing, Mehmet Cengi̇z Han, Ali̇ Sai̇d Durmuş, Aydin Sağliyan, Ci̇han Günay, Mustafa Özkaraca, Fati̇h Mehmet Kandemi̇r, Seli̇m Çomakli, Di̇cle Firat Öztopalan

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to determine the influence of applying Nigella sativa (NS) and Hypericum perforatum (HP) on wound healing. A total of 42 female albino 2-month-old Wistar rats were randomly separated into 3 main groups. Each of these main groups was separated into two subgroups for 7- and 14-day postoperative follow-ups. A total of 84 excisional skin wounds, 5 mm in diameter, were created on the backs of the rats. Placebo cream (control group), 50% NS oil cream, and 50% HP oil cream were applied topically for the first, second, and third group, respectively. The wounds were …

Effects Of Massive Ovulation On Oxidation State And Function Of The Ovaries In Laying Hens, Yu Chang, Jinghai Feng, Minhong Zhang, Liwen Jiang, Lili Zhai, Xiaolan Yang

Turkish Journal of Veterinary & Animal Sciences

Modern laying hens show fast reduction in egg production rate after 60 weeks of age, which is associated with the decline in ovarian function. The aim of this study was to examine the influence of massive ovulation on the oxidation state and ovarian function in hens. In total, 48 Jing Hong hens aged 17 weeks were randomly divided into high ovulation (HO) and low ovulation (LO) groups. The LO hens were exposed to 6 h of light per day (6 L:18 D) up to 32 weeks to delay the initiation of egg production and then were switched to 16 L:8 …

The Comparison Of The Effects Of Intraarticular Injections Of Bovine Amnioticfluid And Hyaluronic Acid On Cartilage Tissue In An Experimental Osteoarthriticrabbit Model: Histopathological And Immunohistochemical Results, Murat Tanrisever, Hati̇ce Eröksüz, Sai̇t Bulut

Turkish Journal of Veterinary & Animal Sciences

In this experimental study, effects of bovine amniotic fluid and hyaluronic acid were compared on an osteoarthritic model induced in rabbit stifle joints. The study was performed on 21 rabbits undergoing anterior cruciate ligament transaction for establishing osteoarthritis. Four weeks later, the rabbits were divided into 3 equal groups of 7 animals each. The first group of rabbits received intraarticular injection of 0.5 mL of bovine amniotic fluid (BAF) three times at 1-week intervals. The second group of rabbits received intraarticular injection of 0.5 mL of hyaluronic acid (HA) three times at 1-week intervals. The third group of rabbits served …

Effects Of Ketamine On Monoamine Neurotransmittersand Gaba In Embryonic Neurocytes From Fetal Rat, Xinran Li, Mo Liu, Yue Li, Cen Guo, Lina Li, Yanan Li, Yuxin Wang, Yiming Zhang, Li Gao

Turkish Journal of Veterinary & Animal Sciences

The effect of ketamine, a drug commonly used as an anesthetic, on monoaminergic neurotransmitters and GABA in neurocytes from fetal rats has not yet been elucidated. We thus decided to investigate these effects by first evaluating ketamine levels in the cerebrospinal fluid by high-performance liquid chromatography. We thus determined the concentrations of GABA, DA, NE, 5-HT, and 5-HIAA following the addition of four different concentrations of ketamine at day 7 of nerve cell culture. GABA, 5-HT, and 5-HIAA levels were increased after an initial decrease: they showed the lowest levels at 10-15 min and the highest peaks at 45-90 min, …

Ultrastructure Of Rat Umbilical Cord Stroma-Derived Mesenchymal Stem Cells, Ahmet Ceylan, Özge Özgenç, Korhan Altunbaş, Petek Korkusuz, Asuman Özen

Turkish Journal of Veterinary & Animal Sciences

Being multipotent progenitor cells, mesenchymal stem cells continue to attract attention as a promising tool for cell-based therapy and tissue engineering. However, research on the ultrastructure of these cells is rather scarce. This study was aimed at the investigation of the transmission electron microscopic ultrastructure and morphology of mesenchymal stem cells isolated from the stroma of rat umbilical cord using the explant culture method. At the end of the third passage, some of the cells isolated from the stroma of the umbilical cord were harvested for electron microscopic examination. The remaining cells were induced for osteogenic, chondrogenic, and adipogenic differentiation. …

Effect Of Feeding Broken Rice And Distillers Dried Grains With Solubles In A Flaxseed-Based Diet On The Growth Performance, Production Efficiency, Carcass Characteristics, Sensory Evaluation Of Meat, And Serum Biochemistry Of Broiler Chickens, Nasir Akbar Mir, Praveen Kumar Tyagi, Ashim Kumar Biswas, Pramod Kumar Tyagi, Asit Baran Mandal, Faneshwar Kumar, Chandra Deo, Avishek Biswas

Turkish Journal of Veterinary & Animal Sciences

This study investigated the effect of broken rice and distillers dried grains with solubles (DDGS) in a flaxseed-based diet on the growth performance, production efficiency, carcass characteristics, sensory evaluation of meat, and serum biochemistry of broiler chickens. Six dietary treatments were formulated with no flaxseed in the first group (C), 10% in another five (T1, T2, T3, T4, and T5), and 20% broken rice, 40% broken rice, 5% DDGS, and 10% DDGS in the T2, T3, T4, and T5 treatment groups, respectively. Each treatment was allocated 6 replicates with 8 chicks in each at random. Treatment with 10% flaxseed and …

Comparative Clinical Presentation And Pathology Of Some Organs In Experimentally Infected Yankasa Sheep With Trypanosoma Vivax And Trypanosoma Congolense From Nigeria, Christopher Ogbaje, Idris Lawal, Joseph Ajanusi

Turkish Journal of Veterinary & Animal Sciences

A study was conducted to compare the clinical presentation and pathology of some organs associated with experimental infection of Nigeria field isolates of Trypanosoma vivax and Trypanosoma congolense in the Yankasa breed of sheep. Thirty adult Yankasa sheep of both sexes were used. They were screened for hemo-, endo-, and ectoparasites and treated and conditioned for 2 weeks. They were divided into 5 groups (A, B, C, E, and F) of 6 animals each. Two groups each (A, B and E, F) were infected with 2 mL of infected blood containing approximately 2.0 x 106 of T. vivax and T. …

Effects Of Housing Environment On Social Isolation Response,Weaning Stress, And Immune Reaction In Goat Kids, Cemi̇l Tölü, Semra Göktürk, Türker Savaş

Turkish Journal of Veterinary & Animal Sciences

This study investigated how environmental structuring in the growing units of goat kids influences their ability to cope with social isolation and weaning-induced stress. Two experimental groups were arranged in an enriched (E) or barren (B) environment and each of the two groups had 10 female and 10 male kids. During the social isolation, the goat kids in group E started bleating earlier (P = 0.1612) and more frequently (P = 0.0125). Goat kids also bleated earlier (P = 0.0005) and more often after the weaning period (P = 0.0006). Only the rearing behavior of the groups exhibited significant differences …

The Effects Of Individual Weaning Based On Birth Weight On Growth Performance And Milk Yield In Dairy Goats, Özdal Gökdal, Ali̇ Kemali̇ Özuğur, Okan Atay, Vadullah Eren

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of using birth weight of kids for weaning on growth and milk yield characteristics of Saanen and Alpine goats. A total of 24 does and their 40 kids were assigned to weaning groups when the kids reached three (X3) or four times (X4) their individual birth weights. The experimental design was a 2 x 2 factorial design with two weaning stages (X3 vs. X4) and two breeds (S: Saanen; A: Alpine). The averages of weaning age of the SX3, AX3, SX4, and AX4 kids were determined as 42.75, 42.21, 53.15, …

Efficacy Of Repeatedly Used Cidr Device In Cattle Reproduction: A Metaanalysisreview Of Progesterone Concentration And Conception Rate, Muhammad Furqan Asghar Chacher, Armağan Çolak, Armağan Hayirli

Turkish Journal of Veterinary & Animal Sciences

n order to reduce estrus synchronization cost, utilization of residual progesterone (P4) in the used controlled internal drug release (CIDR) device upon sanitization can be possible. Two datasets were compiled from 8 and 7 research articles involving 3434 (2653 heifers + 771 lactating cows + 10 ovariectomized cows) and 7301 cattle (3879 heifers + 3422 lactating cows) to evaluate blood P4 concentration and conception rate, respectively, in response to repeatedly used CIDR devices after sanitization via autoclaving and disinfection. As the number of CIDR usages increased, blood P4 concentration decreased linearly (y = -0.484X + 3.135, R2 = 0.99, P < 0.001). Blood P4 concentration (1.52 vs. 2.20 ng/mL; P < 0.002) and conception rate (40.76 vs. 32.96%, P < 0.05) for lactating cows were lower than those for heifers. The sanitization method did not affect blood P4 concentration, but reused CIDR device subjected to disinfection was associated with reduced conception rate. In summary, depending upon the initial P4 load (1.38-1.90 g), the CIDR device could be used twice in lactating cows and four times in heifers after autoclaving to achieve target blood P4 concentrations.

In Vitro Porcine Blastocyst Development In Three-Dimensional Alginate Hydrogels, Jeremy R. Miles, Taylor D. Laughlin, Catherine Sargus-Patino, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Appropriate embryonic and fetal development significantly impact pregnancy success and, therefore, the efficiency of swine production. The pre-implantation period of porcine pregnancy is characterized by several developmental hallmarks, which are initiated by the dramatic morphological change that occurs as pig blastocysts elongate from spherical to filamentous blastocysts. Deficiencies in blastocyst elongation contribute to approximately 20% of embryonic loss, and have a direct influence on within-litter birth weight variation. Although factors identified within the uterine environment may play a role in blastocyst elongation, little is known about the exact mechanisms by which porcine (or other species’) blastocysts initiate and progress through …

Rapid Typing Of Mannheimia Haemolytica Major Genotypes 1 And 2 Using Maldi-Tof Mass Spectrometry, John Dustin Loy, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Genotype 2 M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly differentiate genotype 2 strains in veterinary diagnostic laboratories.

Cytolytic Toxin Production By Staphylococcus Aureus Is Dependent Upon The Activity Of The Protoheme Ix Farnesyltransferase, Emily Stevens, Maisem Laabei, Stewart Gardner, Greg A. Somerville, Ruth C. Massey

School of Veterinary and Biomedical Sciences: Faculty Publications

Staphylococcus aureus is a medically important pathogen with an abundance of virulence factors that are necessary for survival within a host, including the production of cytolytic toxins. The regulation of toxin production is mediated by the Agr quorum sensing system, and a poorly defined post-exponential growth phase signal independent of Agr. As part of a recent genome wide association study (GWAS) to identify novel loci that alter the expression of cytolytic toxins, a polymorphism in the cyoE gene, which encodes a protoheme IX farnesyltransferase, was identified. This enzyme is essential for processing heme into the electron transport chain for use …

Branched Chain Α-Ketoacid Dehydrogenase Kinase 111–130, A T Cell Epitope That Induces Both Autoimmune Myocarditis And Hepatitis In A/J Mice, Bharathi Krishnan, Chandirasegara Massilamany, Rakesh H. Basavalingappa, Arunakumar Gangaplara, Guobin Kang, Qingsheng Li, Francisco A. Uzal, Jennifer L. Strande, Gustavo A. Delhon, Jean-Jack Riethoven, David J. Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Introduction: Organ-specific autoimmune diseases are believed to result from immune responses generated against self-antigens specific to each organ. However, when such responses target antigens expressed promiscuously in multiple tissues, then the immune-mediated damage may be wide spread.

Methods: In this report, we describe a mitochondrial protein, branched chain α-ketoacid dehydrogenase kinase (BCKD_k) that can act as a target autoantigen in the development of autoimmune inflammatory reactions in both heart and liver.

Results: We demonstrate that BCKD_k protein contains at least nine immunodominant epitopes, three of which, BCKD_k 71–90, BCKD_k 111–130 and BCKD_k 141–160, …

Mechanistic Understanding Of N-Glycosylation In Ebola Virus Glycoprotein Maturation And Function, Bin Wang, Yujie Wang, Dylan A. Frabutt, Xihe Zhang, Xiaoyu Yao, Dan Hu, Zhuo Zhang, Chaonan Liu, Shimin Zheng, Shi-Hua Xiang, Yong-Hui Zheng

School of Veterinary and Biomedical Sciences: Faculty Publications

The Ebola virus (EBOV) trimeric envelope glycoprotein (GP) precursors are cleaved into the receptor-binding GP₁ and the fusion-mediating GP₂ subunits and incorporated into virions to initiate infection. GP₁ and GP₂ form heterodimers that have 15 or two N-glycosylation sites (NGSs), respectively. Here we investigated the mechanism of how N-glycosylation contributes to GP expression, maturation, and function. As reported before, we found that, although GP₁ NGSs are not critical, the two GP₂ NGSs, Asn⁵⁶³ and Asn⁶¹⁸, are essential for GP function. Further analysis uncovered that Asn⁵⁶³ and Asn^{618 …}

A Novel Role Of Silibinin As A Putative Epigenetic Modulator In Human Prostate Carcinoma, Ioannis Anestopoulos, Aristeidis P. Sfakianos, Rodrigo Franco, Katerina Chlichlia, Mihalis I. Panayiotidis, David J. Kroll, Aglaia Pappa

School of Veterinary and Biomedical Sciences: Faculty Publications

Silibinin, extracted from milk thistle (Silybum marianum L.), has exhibited considerable preclinical activity against prostate carcinoma. Its antitumor and chemopreventive activities have been associated with diverse effects on cell cycle, apoptosis, and receptor-dependent mitogenic signaling pathways. Here we hypothesized that silibinin’s pleiotropic effects may reflect its interference with epigenetic mechanisms in human prostate cancer cells. More specifically, we have demonstrated that silibinin reduces gene expression levels of the Polycomb Repressive Complex 2 (PRC2) members Enhancer of Zeste Homolog 2 (EZH2), Suppressor of Zeste Homolog 12 (SUZ12), and Embryonic Ectoderm Development (EED) in DU145 and PC3 human prostate cancer cells, …

Ccpa Affects Infectivity Of Staphylococcus Aureus In A Hyperglycemic Environment, Markus Bischoff, Bodo Wonnenberg, Nadine Nippe, Naja J. Nyffenegger-Jann, Meike Voss, Christoph Beisswenger, Cord Sunderkotter, Virginie Molle, Quoc Thai Dinh, Frank Lammert, Robert Bals, Mathias Herrmann, Greg A. Somerville, Thomas Tschernig, Rosmarie Gaupp

School of Veterinary and Biomedical Sciences: Faculty Publications

Many bacteria regulate the expression of virulence factors via carbon catabolite responsive elements. In Gram-positive bacteria, the predominant mediator of carbon catabolite repression is the catabolite control protein A (CcpA). Hyperglycemia is a widespread disorder that predisposes individuals to an array of symptoms and an increased risk of infections. In hyperglycemic individuals, the bacterium Staphylococcus aureus causes serious, life-threatening infections. The importance of CcpA in regulating carbon catabolite repression in S. aureus suggests it may be important for infections in hyperglycemic individuals. To test this suggestion, hyperglycemic non-obese diabetic (NOD; blood glucose level ≥20 mM) mice were challenged with the …