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Veterinary Medicine Commons™

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2017

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Articles 271 - 300 of 366

Full-Text Articles in Veterinary Medicine

Β1-Adrenergic Receptor Contains Multiple IaK And IeK Binding Epitopes That Induce T Cell Responses With Varying Degrees Of Autoimmune Myocarditis In A/J Mice, Rakesh H. Basavalingappa, Chandirasegaran Massilamany, Bharathi Krishnan, Arunakumar Gangaplara, Rajkumar A. Rajasekaran, Muhammad Z. Afzal, Jean-Jack Riethoven, Jennifer L. Strande, David J. Steffen, Jay Reddy Jan 2017

Β1-Adrenergic Receptor Contains Multiple IaK And IeK Binding Epitopes That Induce T Cell Responses With Varying Degrees Of Autoimmune Myocarditis In A/J Mice, Rakesh H. Basavalingappa, Chandirasegaran Massilamany, Bharathi Krishnan, Arunakumar Gangaplara, Rajkumar A. Rajasekaran, Muhammad Z. Afzal, Jean-Jack Riethoven, Jennifer L. Strande, David J. Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Myocarditis/dilated cardiomyopathy (DCM) patients can develop autoantibodies to various cardiac antigens and one major antigen is β1-adrenergic receptor (β1AR). Previous reports indicate that animals immunized with a β1AR fragment encompassing, 197–222 amino acids for a prolonged period can develop DCM by producing autoantibodies, but existence of T cell epitopes, if any, were unknown. Using A/J mice that are highly susceptible to lymphocytic myocarditis, we have identified β1AR 171–190, β1AR 181–200, and β1AR 211–230 as the major T cell epitopes that bind major histocompatibility complex class II/IAk or IEk …

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The Effect Of Feed Form On Diet Digestibility And Cecal Parameters In Rabbits, Isabella Corsato Alvarenga, Charles Gregory Aldrich, Micah Kohles Jan 2017

The Effect Of Feed Form On Diet Digestibility And Cecal Parameters In Rabbits, Isabella Corsato Alvarenga, Charles Gregory Aldrich, Micah Kohles

School of Veterinary and Biomedical Sciences: Faculty Publications

Fifteen New Zealand rabbits were randomly assigned to one of 3 dietary treatment groups of 5 animals each and fed pelleted, extruded, or muesli diets in a completely randomized design experiment. Rabbits were placed in individual cages with ad libitum access to water and food for 45 days acclimation followed by 30 days experimental period. Feed intake of rabbits fed pelleted and extruded diets was greater (p < 0.05) than rabbits fed the muesli diet (125.6 and 130.4 vs. 91.9 g/d), but weight change and feed efficiency were not affected by treatment. Diet digestibility among the treatments was inconsistent when comparing results obtained from total fecal collection and AIA (please define) as an internal marker. Rabbits fed extruded and pelleted diets had lower (p < 0.05) cecal pH (6.42 and 6.38 vs. 7.02, respectively), and higher (p < 0.05) production of SCFA (18.5 and 19.0 vs. 11.7 mM, respectively) than those fed muesli. The fermentation products from rabbits fed pelleted and extruded diets had a greater proportion of butyrate and less propionate than rabbits fed muesli. The results of this study indicate that the basal dietary composition had a greater impact on diet utilization and cecal fermentation than food form.

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Mechanistic Understanding Of N-Glycosylation In Ebola Virus Glycoprotein Maturation And Function, Bin Wang, Yujie Wang, Dylan A. Frabutt, Xihe Zhang, Xiaoyu Yao, Dan Hu, Zhuo Zhang, Chaonan Liu, Shimin Zheng, Shi-Hua Xiang, Yong-Hui Zheng Jan 2017

Mechanistic Understanding Of N-Glycosylation In Ebola Virus Glycoprotein Maturation And Function, Bin Wang, Yujie Wang, Dylan A. Frabutt, Xihe Zhang, Xiaoyu Yao, Dan Hu, Zhuo Zhang, Chaonan Liu, Shimin Zheng, Shi-Hua Xiang, Yong-Hui Zheng

School of Veterinary and Biomedical Sciences: Faculty Publications

The Ebola virus (EBOV) trimeric envelope glycoprotein (GP) precursors are cleaved into the receptor-binding GP1 and the fusion-mediating GP2 subunits and incorporated into virions to initiate infection. GP1 and GP2 form heterodimers that have 15 or two N-glycosylation sites (NGSs), respectively. Here we investigated the mechanism of how N-glycosylation contributes to GP expression, maturation, and function. As reported before, we found that, although GP1 NGSs are not critical, the two GP2 NGSs, Asn563 and Asn618, are essential for GP function. Further analysis uncovered that Asn563 and Asn618 …

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A Novel Role Of Silibinin As A Putative Epigenetic Modulator In Human Prostate Carcinoma, Ioannis Anestopoulos, Aristeidis P. Sfakianos, Rodrigo Franco, Katerina Chlichlia, Mihalis I. Panayiotidis, David J. Kroll, Aglaia Pappa Jan 2017

A Novel Role Of Silibinin As A Putative Epigenetic Modulator In Human Prostate Carcinoma, Ioannis Anestopoulos, Aristeidis P. Sfakianos, Rodrigo Franco, Katerina Chlichlia, Mihalis I. Panayiotidis, David J. Kroll, Aglaia Pappa

School of Veterinary and Biomedical Sciences: Faculty Publications

Silibinin, extracted from milk thistle (Silybum marianum L.), has exhibited considerable preclinical activity against prostate carcinoma. Its antitumor and chemopreventive activities have been associated with diverse effects on cell cycle, apoptosis, and receptor-dependent mitogenic signaling pathways. Here we hypothesized that silibinin’s pleiotropic effects may reflect its interference with epigenetic mechanisms in human prostate cancer cells. More specifically, we have demonstrated that silibinin reduces gene expression levels of the Polycomb Repressive Complex 2 (PRC2) members Enhancer of Zeste Homolog 2 (EZH2), Suppressor of Zeste Homolog 12 (SUZ12), and Embryonic Ectoderm Development (EED) in DU145 and PC3 human prostate cancer cells, …

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Pathogenesis, Molecular Genetics, And Genomics Of Mycobacterium Avium Subsp. Paratuberculosis, The Etiologic Agent Of Johne’S Disease, Govardhan Rathnaiah, Denise K. Zinniel, John P. Bannantine, Judith R. Stabel, Yrjo T. Grohn, Michael T. Collins, Raul G. Barletta Jan 2017

Pathogenesis, Molecular Genetics, And Genomics Of Mycobacterium Avium Subsp. Paratuberculosis, The Etiologic Agent Of Johne’S Disease, Govardhan Rathnaiah, Denise K. Zinniel, John P. Bannantine, Judith R. Stabel, Yrjo T. Grohn, Michael T. Collins, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne’s disease in ruminants causing chronic diarrhea, malnutrition, and muscular wasting. Neonates and young animals are infected primarily by the fecal–oral route. MAP attaches to, translocates via the intestinal mucosa, and is phagocytosed by macrophages. The ensuing host cellular immune response leads to granulomatous enteritis characterized by a thick and corrugated intestinal wall. We review various tissue culture systems, ileal loops, and mice, goats, and cattle used to study MAP pathogenesis. MAP can be detected in clinical samples by microscopy, culturing, PCR, and an enzyme-linked immunosorbent assay. There are commercial …

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Ccpa Affects Infectivity Of Staphylococcus Aureus In A Hyperglycemic Environment, Markus Bischoff, Bodo Wonnenberg, Nadine Nippe, Naja J. Nyffenegger-Jann, Meike Voss, Christoph Beisswenger, Cord Sunderkotter, Virginie Molle, Quoc Thai Dinh, Frank Lammert, Robert Bals, Mathias Herrmann, Greg A. Somerville, Thomas Tschernig, Rosmarie Gaupp Jan 2017

Ccpa Affects Infectivity Of Staphylococcus Aureus In A Hyperglycemic Environment, Markus Bischoff, Bodo Wonnenberg, Nadine Nippe, Naja J. Nyffenegger-Jann, Meike Voss, Christoph Beisswenger, Cord Sunderkotter, Virginie Molle, Quoc Thai Dinh, Frank Lammert, Robert Bals, Mathias Herrmann, Greg A. Somerville, Thomas Tschernig, Rosmarie Gaupp

School of Veterinary and Biomedical Sciences: Faculty Publications

Many bacteria regulate the expression of virulence factors via carbon catabolite responsive elements. In Gram-positive bacteria, the predominant mediator of carbon catabolite repression is the catabolite control protein A (CcpA). Hyperglycemia is a widespread disorder that predisposes individuals to an array of symptoms and an increased risk of infections. In hyperglycemic individuals, the bacterium Staphylococcus aureus causes serious, life-threatening infections. The importance of CcpA in regulating carbon catabolite repression in S. aureus suggests it may be important for infections in hyperglycemic individuals. To test this suggestion, hyperglycemic non-obese diabetic (NOD; blood glucose level ≥20 mM) mice were challenged with the …

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In Vitro Porcine Blastocyst Development In Three-Dimensional Alginate Hydrogels, Jeremy R. Miles, Taylor D. Laughlin, Catherine Sargus-Patino, Angela K. Pannier Jan 2017

In Vitro Porcine Blastocyst Development In Three-Dimensional Alginate Hydrogels, Jeremy R. Miles, Taylor D. Laughlin, Catherine Sargus-Patino, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Appropriate embryonic and fetal development significantly impact pregnancy success and, therefore, the efficiency of swine production. The pre-implantation period of porcine pregnancy is characterized by several developmental hallmarks, which are initiated by the dramatic morphological change that occurs as pig blastocysts elongate from spherical to filamentous blastocysts. Deficiencies in blastocyst elongation contribute to approximately 20% of embryonic loss, and have a direct influence on within-litter birth weight variation. Although factors identified within the uterine environment may play a role in blastocyst elongation, little is known about the exact mechanisms by which porcine (or other species’) blastocysts initiate and progress through …

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Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep Jan 2017

Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep

Chulalongkorn University Theses and Dissertations (Chula ETD)

Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …

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Genetic Characterization Of S1 Gene Of Infectious Bronchitis Virus Isolated From Chickens In Thailand During 2014-2016, Sirorat Munyahongse Jan 2017

Genetic Characterization Of S1 Gene Of Infectious Bronchitis Virus Isolated From Chickens In Thailand During 2014-2016, Sirorat Munyahongse

Chulalongkorn University Theses and Dissertations (Chula ETD)

Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is a highly contagious respiratory disease in chickens, causing significant economic losses in poultry industry worldwide. IBV has a high frequency of genetic mutation and recombination in its genome, particularly in the S1 gene, resulting in the emergence of several new IBV variants. This potentially affects the effectiveness of currently used IBV vaccine. Therefore, the continuous monitoring of the currently circulating IBV strains is essential. In this study, the genetic characteristic of IBV strains circulating in Thai chicken flocks during 2014-2016 was investigated by analysis of the complete S1 gene. A …

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Understanding The Effect Of Bvdv On Innate Immune Response Of Neutrophils, Neelu Singh Thakur Jan 2017

Understanding The Effect Of Bvdv On Innate Immune Response Of Neutrophils, Neelu Singh Thakur

Electronic Theses and Dissertations

Bovine viral diarrhea virus (BVDV) is one of the highly prevalent and economically important diseases of cattle industry worldwide. The two major consequences of this disease are persistent infection and immunosuppression. Several studies have been done to determine the underline mechanisms of BVDV-induced immunosuppression targeting antigen presenting cells, adaptive immune system cells and cytokine gene expression. However, very little research has been done to determine the effect of BVDV on neutrophils. Neutrophils are one of the most abundant while blood cells (WBC) in the peripheral blood, which play a critical role in the innate as well as adaptive immune response. …

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Effects Of 17Β Estradiol In The Metabolism And Morphology Of Bluegill Sunfish (Lepomis Macrochirus), Neeta Parajulee Karki Jan 2017

Effects Of 17Β Estradiol In The Metabolism And Morphology Of Bluegill Sunfish (Lepomis Macrochirus), Neeta Parajulee Karki

Masters Theses

Fish natural habitats are increasingly contaminated with various estrogenic compounds, including 17β estradiol (E2). This compound causes adverse effects on the reproductive system of male fish; however, the effects of E2 on other aspects of fish metabolism, morphology and histopathological changes in internal organs are not well known. The objective of this study is to evaluate the effects of E2 exposure on the basal and stressed metabolic rate, morphological changes in body shapes, and histological changes in the liver tissues of sunfish species. Fish were held individually in ten gallon tanks under two treatments of 40 and 80 ng/L and …

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Vmed 646: Animal Physiology Ii—A Peer Review Of Teaching Project Benchmark Portfolio, Renee M. Mcfee Jan 2017

Vmed 646: Animal Physiology Ii—A Peer Review Of Teaching Project Benchmark Portfolio, Renee M. Mcfee

UNL Faculty Course Portfolios

This Peer Review of Teaching Project portfolio focuses on the Animal Physiology II course which is required for first year veterinary medicine students. Weekly quizzes assess baseline knowledge and had been administered individually and in groups. I hypothesized the discontinuation of group quizzes would increase student effort when preparing for quizzes. Unit exams involve scenario-based questions and require students to apply information. I hypothesized the implementation of group exams would help under-performing students improve their ability to apply information they had learned. Exams were still taken individually prior to being taken in groups to encourage adequate preparation. Student impacts were …

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Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ Jan 2017

Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

Pestivirus infections have a huge economic impact on livestock production. In 2014, an aborted fetus from a sheep flock suffering from abortus and diarrhea was submitted for virological diagnosis. Due to the positive result of the sample for pestivirus and continuing clinical symptoms in the flock, all of the animals were sampled individually. Blood samples for serum and peripheral blood mononuclear cells were collected from 93 animals (5 rams, 26 lambs, and 62 sheep). During sampling 1 ocular and 4 rectal swab samples were obtained from lambs that had a clinical eye problem and diarrhea, respectively. Additionally 5 aborted fetuses …

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Design Of Probiotic Dry Fermented Sausage (Sucuk) Production Withmicroencapsulated And Free Cells Of Lactobacillus Rhamnosus, Emel Ünal, Zerri̇n Ergi̇nkaya, Süleyman Polat, Emi̇r Ayşe Özer Jan 2017

Design Of Probiotic Dry Fermented Sausage (Sucuk) Production Withmicroencapsulated And Free Cells Of Lactobacillus Rhamnosus, Emel Ünal, Zerri̇n Ergi̇nkaya, Süleyman Polat, Emi̇r Ayşe Özer

Turkish Journal of Veterinary & Animal Sciences

The present study was performed to design sucuk production with microencapsulated and free cells of Lactobacillus rhamnosus and to investigate the effect of this production method on probiotic viability and quality characteristics on the final product. L. rhamnosus was microencapsulated with an optimal coating material combination by using response surface methodology and subsequently used in sucuk production as a probiotic culture. Microencapsulation protected L. rhamnosus against gastric and other stress conditions in sucuk composition. Sucuk production with microencapsulated L. rhamnosus was found to be similar to traditional sucuk in terms of textural, physicochemical, and sensorial properties. Novel sucuk production was …

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Effect Of Utilization Of A Controlled-Release Intraruminal Monensin Device On Drymatter Intake, Milk Yield, And Some Reproductive Parameters In Dairy Cows, Seçki̇n Salar, Oğuz Çalişici, Duygu Çalişici, İdi̇l Baştan, Ayhan Baştan Jan 2017

Effect Of Utilization Of A Controlled-Release Intraruminal Monensin Device On Drymatter Intake, Milk Yield, And Some Reproductive Parameters In Dairy Cows, Seçki̇n Salar, Oğuz Çalişici, Duygu Çalişici, İdi̇l Baştan, Ayhan Baştan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to examine the effect of the prepartal administration of the monensin release capsule on dry matter intake (DMI), milk yield, and some reproductive parameters of dairy cows. The cows were divided into study (n = 35) and control (n = 41) groups. The study group cows were administered the intraruminal-monensin releasing capsules at 21 $\pm$ 3 days before expected calving and the control group cows were not subjected to treatment. Monthly mean DMI; cumulative milk yield at 30, 45, and 60 days in milk (DIM); peak milk yield; days of 1st AI; days open; …

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Effects Of Housing Environment On Social Isolation Response,Weaning Stress, And Immune Reaction In Goat Kids, Cemi̇l Tölü, Semra Göktürk, Türker Savaş Jan 2017

Effects Of Housing Environment On Social Isolation Response,Weaning Stress, And Immune Reaction In Goat Kids, Cemi̇l Tölü, Semra Göktürk, Türker Savaş

Turkish Journal of Veterinary & Animal Sciences

This study investigated how environmental structuring in the growing units of goat kids influences their ability to cope with social isolation and weaning-induced stress. Two experimental groups were arranged in an enriched (E) or barren (B) environment and each of the two groups had 10 female and 10 male kids. During the social isolation, the goat kids in group E started bleating earlier (P = 0.1612) and more frequently (P = 0.0125). Goat kids also bleated earlier (P = 0.0005) and more often after the weaning period (P = 0.0006). Only the rearing behavior of the groups exhibited significant differences …

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Development Of Leptospiral Outer Membrane Protein-Based Dot-Elisa Assays And Immunoprofiling For Diagnosis Of Acute Canine Leptospirosis, Vamshi Krishna Sriram, Siju Joseph, Ambily Ramachandran, Mini Mangattumuruppel Jan 2017

Development Of Leptospiral Outer Membrane Protein-Based Dot-Elisa Assays And Immunoprofiling For Diagnosis Of Acute Canine Leptospirosis, Vamshi Krishna Sriram, Siju Joseph, Ambily Ramachandran, Mini Mangattumuruppel

Turkish Journal of Veterinary & Animal Sciences

The present study was carried out to evaluate outer membrane protein (OMP)-based relatively simple dot-ELISA assays for the diagnosis of acute canine leptospirosis and to identify the differential expression of leptospiral OMPs for identifying the markers of acute infection, if any. Among 91 canine serum samples screened, 63 samples were found to be positive by MAT. All the samples were tested by OMP-based IgM and IgG dot ELISAs. The sensitivities of IgM and IgG dot ELISA in comparison to MAT (titer: ≥1:400) were found to be 100%, whereas the specificities were found to be 89.71% and 45.31%, respectively. IgM and …

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Effect Of Polymorphisms In Intron 1 Of The Swine Pou1f1gene On Growth And Reproductive Traits, Lyubov Getmantseva, Anatoly Kolosov, Maria Leonova, Aleksander Usatov, Faridun Bakoev, Aleksander Klimenko, Siroj Bakoev Jan 2017

Effect Of Polymorphisms In Intron 1 Of The Swine Pou1f1gene On Growth And Reproductive Traits, Lyubov Getmantseva, Anatoly Kolosov, Maria Leonova, Aleksander Usatov, Faridun Bakoev, Aleksander Klimenko, Siroj Bakoev

Turkish Journal of Veterinary & Animal Sciences

The most prospective and actual trend in farm animal breeding is studying gene polymorphisms affecting the productive traits. The objective of this investigation was to study the effect of polymorphisms in intron 1 of the swine POU1F1 gene on number of days to 100 kg (days to 100 kg), length of body (LB), backfat thickness (BF), and number of piglets born alive (NBA). Research was conducted on purebred pigs of Landrace (n = 80) and Duroc (n = 100) and crossbred pigs (Landrace x Large White) (n = 192). Insertions/deletions in intron 1 of the POU1F1 gene were defined by …

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Effect Of Feeding Broken Rice And Distillers Dried Grains With Solubles In A Flaxseed-Based Diet On The Growth Performance, Production Efficiency, Carcass Characteristics, Sensory Evaluation Of Meat, And Serum Biochemistry Of Broiler Chickens, Nasir Akbar Mir, Praveen Kumar Tyagi, Ashim Kumar Biswas, Pramod Kumar Tyagi, Asit Baran Mandal, Faneshwar Kumar, Chandra Deo, Avishek Biswas Jan 2017

Effect Of Feeding Broken Rice And Distillers Dried Grains With Solubles In A Flaxseed-Based Diet On The Growth Performance, Production Efficiency, Carcass Characteristics, Sensory Evaluation Of Meat, And Serum Biochemistry Of Broiler Chickens, Nasir Akbar Mir, Praveen Kumar Tyagi, Ashim Kumar Biswas, Pramod Kumar Tyagi, Asit Baran Mandal, Faneshwar Kumar, Chandra Deo, Avishek Biswas

Turkish Journal of Veterinary & Animal Sciences

This study investigated the effect of broken rice and distillers dried grains with solubles (DDGS) in a flaxseed-based diet on the growth performance, production efficiency, carcass characteristics, sensory evaluation of meat, and serum biochemistry of broiler chickens. Six dietary treatments were formulated with no flaxseed in the first group (C), 10% in another five (T1, T2, T3, T4, and T5), and 20% broken rice, 40% broken rice, 5% DDGS, and 10% DDGS in the T2, T3, T4, and T5 treatment groups, respectively. Each treatment was allocated 6 replicates with 8 chicks in each at random. Treatment with 10% flaxseed and …

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Comparative Clinical Presentation And Pathology Of Some Organs In Experimentally Infected Yankasa Sheep With Trypanosoma Vivax And Trypanosoma Congolense From Nigeria, Christopher Ogbaje, Idris Lawal, Joseph Ajanusi Jan 2017

Comparative Clinical Presentation And Pathology Of Some Organs In Experimentally Infected Yankasa Sheep With Trypanosoma Vivax And Trypanosoma Congolense From Nigeria, Christopher Ogbaje, Idris Lawal, Joseph Ajanusi

Turkish Journal of Veterinary & Animal Sciences

A study was conducted to compare the clinical presentation and pathology of some organs associated with experimental infection of Nigeria field isolates of Trypanosoma vivax and Trypanosoma congolense in the Yankasa breed of sheep. Thirty adult Yankasa sheep of both sexes were used. They were screened for hemo-, endo-, and ectoparasites and treated and conditioned for 2 weeks. They were divided into 5 groups (A, B, C, E, and F) of 6 animals each. Two groups each (A, B and E, F) were infected with 2 mL of infected blood containing approximately 2.0 x 106 of T. vivax and T. …

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Ultrastructure Of Apoptotic T Lymphocytes And Thymicepithelial Cells In Early Postnatal Pig Thymus, Ahmet Ceylan, Belma Alabay Jan 2017

Ultrastructure Of Apoptotic T Lymphocytes And Thymicepithelial Cells In Early Postnatal Pig Thymus, Ahmet Ceylan, Belma Alabay

Turkish Journal of Veterinary & Animal Sciences

The thymus is a primary lymphoid organ that provides the microenvironment required for the development of T lymphocytes. Thymic epithelial cells (TECs) are among the most critical components in the thymic microenvironment supporting thymocyte selection and maturation. Apoptosis is primarily a physiologic process that plays a major role during the development of T lymphocytes. The first purpose of this study was to examine morphological changes in apoptotic thymocytes by transmission electron microscope in piglets. The second purpose was to reveal the morphological typology of TECs in piglets. Tissue samples of the thymus were taken from healthy Yorkshire race pigs of …

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The Effect Of Combining Vitamin E And C On The Viability Improvement Of Transfected Ovine Spermatogonial Stem Cells After Cryopreservation And Thawing, Hosein Shabani, Mohammad Zandi, Hamideh Ofogi, Mohammad Reza Sanjabi, Khosro Hoseini Pajooh Jan 2017

The Effect Of Combining Vitamin E And C On The Viability Improvement Of Transfected Ovine Spermatogonial Stem Cells After Cryopreservation And Thawing, Hosein Shabani, Mohammad Zandi, Hamideh Ofogi, Mohammad Reza Sanjabi, Khosro Hoseini Pajooh

Turkish Journal of Veterinary & Animal Sciences

The present study aimed to identify whether vitamin E and C and their combination in basal freezing medium improved the viability of postthaw transfected spermatogonial stem cells (SSCs). For this purpose, SSCs were harvested by enzymatic digestion twice. After enrichment and culture on Sertoli cell feeder layer, SSCs were characterized by using alkaline phosphatase staining and expression of oct-4 and c-kit genes as specific stem cell markers. As to the transfection of SSCs, different concentrations of DNA (0.2, 0.4, and 0.8 μg) and turbofect (0.4 and 0.8 μL) in 100 μL of medium were studied. The results showed that cryopreservation …

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Gene Cloning And Expression Analysis Of Mitochondrial Glutathione Reductase From Arabian Camel (Camelus Dromedarius) Liver In Escherichia Coli, Mona Alharbi, Abdulrahman Mohammad Alsenaidy, Sooad Aldaihan, Anwar Ahmed Jan 2017

Gene Cloning And Expression Analysis Of Mitochondrial Glutathione Reductase From Arabian Camel (Camelus Dromedarius) Liver In Escherichia Coli, Mona Alharbi, Abdulrahman Mohammad Alsenaidy, Sooad Aldaihan, Anwar Ahmed

Turkish Journal of Veterinary & Animal Sciences

Glutathione reductase (GR) is highly conserved among diverse taxa and has important biochemical functions. These functions may facilitate survival in harsh conditions, but the role of GR from the liver of the Arabian camel (Camelus dromedarius) is unknown. In this study, the mitochondrial glutathione reductase gene (Gsr) from C. dromedarius liver was cloned and highly expressed in Escherichia coli (Jm109) to gain insight into GR functions in the liver. After amplification of the cDNA encoding the functional unit of Gsr (1.2 kb), the products were cloned into the PGEM-T Easy and PET28a vectors. Gsr expression was confirmed using an immunoblotting …

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Effects Of Prepartum Treatment With Monensin Or Propylene Glycol Mixed With Concentrate On Milk Yield And Blood Nefa And Bhba Levels In Dairy Cows, Zafer Meci̇toğlu, Sevi̇m Kasap, Mehmet Levent Özdüven, Muhi̇tti̇n Özder, Engi̇n Kennerman Jan 2017

Effects Of Prepartum Treatment With Monensin Or Propylene Glycol Mixed With Concentrate On Milk Yield And Blood Nefa And Bhba Levels In Dairy Cows, Zafer Meci̇toğlu, Sevi̇m Kasap, Mehmet Levent Özdüven, Muhi̇tti̇n Özder, Engi̇n Kennerman

Turkish Journal of Veterinary & Animal Sciences

Sixty multiparous Holstein-Friesian dairy cows made up the study sample. Group M (n = 20) received 300 mg/day monensin (Kexxtone, Elanco) for 21 days prior to the expected calving date. Group PGC (n = 20) received propylene glycol, mixed with concentrate and fed separately from total mixed ration (TMR) twice a day in the last 21 days of gestation. The control group, Group C (n = 20) did not receive any treatment prior to parturition. Cows in all three groups received PG (300 mL/day) for 5 days after parturition. Blood samples were collected 21 and 7 days prior to the …

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The Effects Of Individual Weaning Based On Birth Weight On Growth Performance And Milk Yield In Dairy Goats, Özdal Gökdal, Ali̇ Kemali̇ Özuğur, Okan Atay, Vadullah Eren Jan 2017

The Effects Of Individual Weaning Based On Birth Weight On Growth Performance And Milk Yield In Dairy Goats, Özdal Gökdal, Ali̇ Kemali̇ Özuğur, Okan Atay, Vadullah Eren

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of using birth weight of kids for weaning on growth and milk yield characteristics of Saanen and Alpine goats. A total of 24 does and their 40 kids were assigned to weaning groups when the kids reached three (X3) or four times (X4) their individual birth weights. The experimental design was a 2 x 2 factorial design with two weaning stages (X3 vs. X4) and two breeds (S: Saanen; A: Alpine). The averages of weaning age of the SX3, AX3, SX4, and AX4 kids were determined as 42.75, 42.21, 53.15, …

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Infectious Epididymitis Caused By Brucella Ovis In Croatian Sheep Flocks, Zeljko Cvetnic, Maja Zdelar-Tuk, Sanja Duvnjak, Miroslav Benic, Zeljko Mihaljevic, Boris Habrun, Irena Reil, Marija Cvetnic, Silvio Spicic Jan 2017

Infectious Epididymitis Caused By Brucella Ovis In Croatian Sheep Flocks, Zeljko Cvetnic, Maja Zdelar-Tuk, Sanja Duvnjak, Miroslav Benic, Zeljko Mihaljevic, Boris Habrun, Irena Reil, Marija Cvetnic, Silvio Spicic

Turkish Journal of Veterinary & Animal Sciences

The distribution of infection by the species Brucella ovis in rams and abortion cases in sheep in Croatia was investigated in the period from 2011 to 2015. The investigation relied on serological testing in rams and sheep after abortion conducted using a complement fixation test and immunosorbent assay conducted with bacteriological testing of the testes in seropositive rams and aborted material. Seropositive reactions were confirmed in 1100 (6%) of rams samples and in 12 (2.3%) of ewe samples. Isolated strains were also confirmed on the molecular level. In 10 (45.5%) animals asymmetry of the scrotum and unilateral enlargement of the …

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Fascia Lata Flap To Repair Perineal Hernia In Dogs: A Preliminary Study, Simone Guérios, Juliana Monteiro, Daniella Matos Da Silva Jan 2017

Fascia Lata Flap To Repair Perineal Hernia In Dogs: A Preliminary Study, Simone Guérios, Juliana Monteiro, Daniella Matos Da Silva

Turkish Journal of Veterinary & Animal Sciences

Perineal hernia (PH) in dogs normally requires surgical reconstruction of the pelvic diaphragm. The use of fascia lata grafts has been reported and described in previous studies for the correction of PH after failure of the conventional herniorrhaphy. The purpose of this report is to describe a modified technique of fascia lata flap (FLP) for PH repair in dogs. Six client-owned male dogs with PH were included in the study. PH was repaired with a FLP obtained from the dog's ipsilateral thigh and sutured directly into the perineal defect. Caudal scrotal orchiectomy was performed in all dogs that were not …

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Efficacy Of Repeatedly Used Cidr Device In Cattle Reproduction: A Metaanalysisreview Of Progesterone Concentration And Conception Rate, Muhammad Furqan Asghar Chacher, Armağan Çolak, Armağan Hayirli Jan 2017

Efficacy Of Repeatedly Used Cidr Device In Cattle Reproduction: A Metaanalysisreview Of Progesterone Concentration And Conception Rate, Muhammad Furqan Asghar Chacher, Armağan Çolak, Armağan Hayirli

Turkish Journal of Veterinary & Animal Sciences

n order to reduce estrus synchronization cost, utilization of residual progesterone (P4) in the used controlled internal drug release (CIDR) device upon sanitization can be possible. Two datasets were compiled from 8 and 7 research articles involving 3434 (2653 heifers + 771 lactating cows + 10 ovariectomized cows) and 7301 cattle (3879 heifers + 3422 lactating cows) to evaluate blood P4 concentration and conception rate, respectively, in response to repeatedly used CIDR devices after sanitization via autoclaving and disinfection. As the number of CIDR usages increased, blood P4 concentration decreased linearly (y = -0.484X + 3.135, R2 = 0.99, P < 0.001). Blood P4 concentration (1.52 vs. 2.20 ng/mL; P < 0.002) and conception rate (40.76 vs. 32.96%, P < 0.05) for lactating cows were lower than those for heifers. The sanitization method did not affect blood P4 concentration, but reused CIDR device subjected to disinfection was associated with reduced conception rate. In summary, depending upon the initial P4 load (1.38-1.90 g), the CIDR device could be used twice in lactating cows and four times in heifers after autoclaving to achieve target blood P4 concentrations.

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Promising Synergistic Interactions Among Antimicrobials Against Multidrug-Resistant Escherichia Coli Isolated From Animals, Pinar Şahi̇ntürk, Murat Cengi̇z Jan 2017

Promising Synergistic Interactions Among Antimicrobials Against Multidrug-Resistant Escherichia Coli Isolated From Animals, Pinar Şahi̇ntürk, Murat Cengi̇z

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

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First Report Of Brucella Suis Biovar 2 In Outdoor Reared Pigs(Sus Scrofa Domesticus) In Serbia, Jadranka Zutic, Djordje Cvetojevic, Ljubisa Veljovic, Oliver Radanovic, Slobodan Stanojevic, Branislav Kureljusic Jan 2017

First Report Of Brucella Suis Biovar 2 In Outdoor Reared Pigs(Sus Scrofa Domesticus) In Serbia, Jadranka Zutic, Djordje Cvetojevic, Ljubisa Veljovic, Oliver Radanovic, Slobodan Stanojevic, Branislav Kureljusic

Turkish Journal of Veterinary & Animal Sciences

The objective of this work was to present the first case of Brucella suis biovar 2 isolation in outdoor reared pigs in Serbia. B. suis biovar 2 has not previously been detected in Serbia, from either wild boar or outdoor reared pigs. In our case, brucellosis was discovered in the region of Srem, which geographically constitutes a part of northwestern Serbia. Outdoor reared pigs in the Srem region are part of an extensive breeding system in the field and woods. In the course of a random visit to a herd in the above-mentioned area, the animals' owner discovered two aborted …

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