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Veterinary Medicine Commons™

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2016

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Articles 271 - 300 of 320

Full-Text Articles in Veterinary Medicine

Comparison Of The Development Of Mouse Embryos Manipulatedwith Different Biopsy Techniques, Ali̇ Ci̇han Taşkin, Tolga Akkoç, Hakan Sağirkaya, Haydar Bağiş, Sezen Arat Jan 2016

Comparison Of The Development Of Mouse Embryos Manipulatedwith Different Biopsy Techniques, Ali̇ Ci̇han Taşkin, Tolga Akkoç, Hakan Sağirkaya, Haydar Bağiş, Sezen Arat

Turkish Journal of Veterinary & Animal Sciences

Preimplantation genetic diagnosis is the detection of inherited diseases and the sex of embryos before implantation in the practice of human medicine as well as in veterinary medicine. The introduction of experimental animal embryo biopsy techniques has been a milestone in the developmental process of preimplantation genetic diagnosis techniques. The aim of the present study was to evaluate in vivo and in vitro development of embryos after biopsy in an experimental mouse model and to perform comparisons across different biopsy techniques (blastomere biopsy and trophectoderm biopsy). At the end of the study, no significant difference was observed between the blastomere …

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Effect Of Retinol And Retinol Esters On Performance, Egg Quality, And Blood And Egg Vitamin A Levels In Laying Quails, Murat İlhan, Tuba Bülbül Jan 2016

Effect Of Retinol And Retinol Esters On Performance, Egg Quality, And Blood And Egg Vitamin A Levels In Laying Quails, Murat İlhan, Tuba Bülbül

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the effects of retinol and retinol ester supplementation on performance, egg quality, serum and egg oxidative status, and vitamin A levels in laying quails. Three hundred quails were divided into 5 groups. The control group received a basal diet that contained vitamin premix without vitamin A or vitamin A precursors. The treatment groups received dietary supplementation with retinol and retinol esters [retinyl acetate (R-acetate), retinyl palmitate (R-palmitate), retinyl propionate (R-propionate), 3300 IU/kg]. Egg production and feed conversion ratio improved in all experimental groups. Serum β-carotene and malondialdehyde (MDA) levels decreased and serum retinol levels increased …

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Analysis Of Risk Factors In The Management Of Foot-And-Mouth Disease In Turkey, Berri̇n Şentürk, Cengi̇z Yalçin, Aytaç Akçay Jan 2016

Analysis Of Risk Factors In The Management Of Foot-And-Mouth Disease In Turkey, Berri̇n Şentürk, Cengi̇z Yalçin, Aytaç Akçay

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to perform a risk analysis of the management of foot-and-mouth disease (FMD) epidemics in Turkey. The relationship between disease outbreaks and risk was analyzed by evaluating 11 disease risk factors related to the input, processing, production, movement, and marketing infrastructure aspects of the animal production chain. A progressive (hierarchical) clustering analysis method was employed to group the quantitative data obtained for each of the risk variables. The total risk scores were grouped in a dendrogram. There was a strong positive relationship (72%) between risk variables and total outbreak numbers (P < 0.01). Results showed the districts in which the FMD risk is high and indicate where the appropriate measures should be taken to eradicate or at least minimize outbreaks.

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Estimation Of Stray Dog And Cat Populations In Metropolitan Ankara, Turkey, Doğukan Özen, Dankmar Böhning, İsmayi̇l Safa Gürcan Jan 2016

Estimation Of Stray Dog And Cat Populations In Metropolitan Ankara, Turkey, Doğukan Özen, Dankmar Böhning, İsmayi̇l Safa Gürcan

Turkish Journal of Veterinary & Animal Sciences

Stray dogs and cats contribute to serious health problems in human societies around the world. Before any necessary interventions to control the stray dogs and cats, an accurate estimate of their populations should be attained. Yet, there is a very limited number of methods for an estimation. Some of them depend on the identification of marked or counted animals. However, problems arise when it is not possible to identify a previously captured animal. In this paper, we used a different approach to estimate the lower bound for the total number of dogs and cats in consecutively visited settlements that might …

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In Ovo Feeding With Ss-Hydroxy Ss-Methylbutyrate And Broilerperformance, Intestinal Health, And Immunity Status, Ömer Hakan Muğlali, Mustafa Salman, Ayhan Gacar, Fi̇li̇z Akdağ, Mustafa Yavuz Gülbahar, Alper Çi̇ftçi̇, Ertan Emek Onuk, Fi̇li̇z Karadaş, Hüseyi̇n Eseceli̇ Jan 2016

In Ovo Feeding With Ss-Hydroxy Ss-Methylbutyrate And Broilerperformance, Intestinal Health, And Immunity Status, Ömer Hakan Muğlali, Mustafa Salman, Ayhan Gacar, Fi̇li̇z Akdağ, Mustafa Yavuz Gülbahar, Alper Çi̇ftçi̇, Ertan Emek Onuk, Fi̇li̇z Karadaş, Hüseyi̇n Eseceli̇

Turkish Journal of Veterinary & Animal Sciences

The effects of in ovo administration of β-hydroxy-β-methylbutyrate (HMB) on broiler performance, intestinal health, and immunity were investigated. At day 18 of incubation, fertile eggs were divided into negative (no injection) and positive (physiologic serum injection) controls and three HMB treatment groups. HMB solution was injected at 0.1% (Group 1), 0.2% (Group 2), and 0.3% (Group 3) concentrations. There were no significant differences between the groups for live weight gain, feed conversion rate, and hot carcass, breast meat, liver, and heart weight. However, the gizzard weight in Group 1 was significantly higher than those in the other groups (P < 0.05). Although the intestinal villi lengths in the treatment Groups 1 and 3 on day 20 were greater than those in the control groups (P < 0.01), at the end of the experiment there were no significant differences among the groups. For antibody levels, the differences between groups on days 4 and 42 were highly significant (P < 0.01), and the antibody levels in Group 2 were higher than those in the other groups. Separately, growth performance and antibody levels were positively correlated with the length of villi in Group 2 (HMB 0.2%).

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Evaluation Of Renal And Hepatic Functions In Cattle With Subclinical And Clinical Ketosis, Mustafa İssi̇, Yusuf Gül, Onur Başbuğ Jan 2016

Evaluation Of Renal And Hepatic Functions In Cattle With Subclinical And Clinical Ketosis, Mustafa İssi̇, Yusuf Gül, Onur Başbuğ

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to research the changes in renal and hepatic functions of high productive dairy cows diagnosed with subclinical and clinical ketosis. The material of the study consisted of 30 dairy cows of high milk production. Diagnosis of ketosis was made according to anamnesis, physical examination, urine ketone bodies, and serum β-hydroxybutyric acid levels. The animals were divided according to their serum β-hydroxybutyric acid levels into control, subclinical, and clinical ketosis groups, each of which included 10 cows. Serum glucose, urea, creatinine, total protein, aspartate amino transaminase (AST), and total bilirubin levels were determined with an …

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Lactose-Rich Milk Replacer Modifies The Proteome Of Bloodplasma In 2-Week-Old Calves, Alicja Dratwa Chalupnik, Malgorzata Ozgo, Agnieszka Herosimczyk, Adam Lepczynski, Katarzyna Michalek Jan 2016

Lactose-Rich Milk Replacer Modifies The Proteome Of Bloodplasma In 2-Week-Old Calves, Alicja Dratwa Chalupnik, Malgorzata Ozgo, Agnieszka Herosimczyk, Adam Lepczynski, Katarzyna Michalek

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to create a specific pattern of protein expression changes in the plasma of 2-week-old calves treated with excessive lactose doses. Identification of proteins, the expression of which changes during the short-term administration of lactose, may help to exclude or confirm the causes of diarrhea observed in newborn calves. The present study utilized 2-D electrophoresis combined with MALDI-TOF mass spectrometry and identified 6 blood plasma proteins, the expression of which changed under the influence of lactose added to the milk replacer. The pattern of expression of blood plasma proteins, including decreased expression of fibrinogen and …

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Evaluation Of The Reproductive And Metabolic Problems Encountered During Two Breeding Seasons In Barren Arabian Mares, Erol Koca, Erhan Özenç, Esra Şeker, Alper Sevi̇mli̇, Mehmet Rifat Vural, Mehmet Uçar, Şükrü Küplülü Jan 2016

Evaluation Of The Reproductive And Metabolic Problems Encountered During Two Breeding Seasons In Barren Arabian Mares, Erol Koca, Erhan Özenç, Esra Şeker, Alper Sevi̇mli̇, Mehmet Rifat Vural, Mehmet Uçar, Şükrü Küplülü

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the reproductive problems and determine the effect of treatment method on the pregnancy and foaling rates of 27 barren Arabian mares. The mares were examined over two study seasons. Ultrasonography and gynecological examinations were performed for all mares that were unable to conceive during the previous season and mares for which pregnancy was not achieved even after mating with stallions three times during each season. Endometrial swab samples were also obtained for microbiological examination. Cytology and endometrial biopsy samples were obtained from all mares in the first study season. Endometrial biopsy samples …

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The Effect Of Ovulatory Follicle Size At The Time Of Insemination Onpregnancy Rate In Lactating Dairy Cows, Abdulkadi̇r Keski̇n, Gülnaz Meci̇toğlu, Ebru Bi̇len, Bariş Güner, Abdülkadi̇r Orman, Hayretti̇n Okut, Ahmet Gümen Jan 2016

The Effect Of Ovulatory Follicle Size At The Time Of Insemination Onpregnancy Rate In Lactating Dairy Cows, Abdulkadi̇r Keski̇n, Gülnaz Meci̇toğlu, Ebru Bi̇len, Bariş Güner, Abdülkadi̇r Orman, Hayretti̇n Okut, Ahmet Gümen

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were to determine the factors that affect follicle size at the time of artificial insemination (AI) and to detect the relationship between ovulatory follicle size at the time of AI and pregnancy rate in dairy cows. A total of 1428 follicle size measurements were obtained from Holstein-Friesian (HF) and Swedish Red (SR) cows that were inseminated with the fixed-timed AI protocols used in this study. Follicle size was affected by breed (P = 0.0001), milk production (P = 0.01), parity (P = 0.05), and season (P = 0.04). Follicle size was greater (P = 0.0001) …

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Postnatal Expression Profiles Of Phospholipid Hydroperoxide Glutathioneperoxidase In Spermatogenesis In Mice, Ki Youn Jung, Chunmei Lin, Jung-Min Yon, A Young Jung, Seul Gi Park, Sl Bi Chu, Lee Wha Gwon, Sang Yoon Nam Jan 2016

Postnatal Expression Profiles Of Phospholipid Hydroperoxide Glutathioneperoxidase In Spermatogenesis In Mice, Ki Youn Jung, Chunmei Lin, Jung-Min Yon, A Young Jung, Seul Gi Park, Sl Bi Chu, Lee Wha Gwon, Sang Yoon Nam

Turkish Journal of Veterinary & Animal Sciences

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is known to be an antioxidant enzyme that may be implicated in spermatogenesis, but its functional significance in the development of testes remains poorly understood. To demonstrate the roles of PHGPx in spermatogenesis during postnatal development, expression profiles of PHGPx mRNA and protein in testes and epididymides obtained from mice 1-9 and 27 weeks after birth were spatiotemporally analyzed. In testes, PHGPx mRNA first appeared in 2-week-old mice, while its protein was first detected in late spermatocytes and round spermatids from 3-week-old mice and levels were greatly increased in elongated spermatids and immature spermatozoa of …

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Progesterone Concentration In Milk And Blood Serum And Reproductiveefficiency Of Cows After Ovsynch Treatment, Dejan Vukovic, Aleksandar Bozic, Renata Relic, Blagoje Stancic, Dragan Gvozdic, Denis Kucevic Jan 2016

Progesterone Concentration In Milk And Blood Serum And Reproductiveefficiency Of Cows After Ovsynch Treatment, Dejan Vukovic, Aleksandar Bozic, Renata Relic, Blagoje Stancic, Dragan Gvozdic, Denis Kucevic

Turkish Journal of Veterinary & Animal Sciences

An experiment was conducted to investigate the effects of hormonal synchronization of ovulation on progesterone concentrations in milk and blood serum and on reproductive performance. Sixty Holstein-Friesian cows averaging 8000 L over 305 days of lactation were divided into 2 groups of 30 animals. One-factor ANOVA and a t-test of progesterone concentrations in venous serum and milk revealed that the lowest concentrations of progesterone in milk (Ovsynch 5.8 ± 1.0 ng/mL; control 4.5 ± 0.6 ng/mL) and blood serum (Ovsynch 1.3 ± 0.9 ng/mL; control 1.4 ± 0.5 ng/mL) in both groups were found on the day of estrus or …

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Quality Of Oocytes In Prepubertal And Pubertal Swine, Rita Grabowska, Barbara Blaszczyk, Tomasz Stankiewicz, Tomasz Banas, Sarah Hale, Jan Udala Jan 2016

Quality Of Oocytes In Prepubertal And Pubertal Swine, Rita Grabowska, Barbara Blaszczyk, Tomasz Stankiewicz, Tomasz Banas, Sarah Hale, Jan Udala

Turkish Journal of Veterinary & Animal Sciences

In vitro fertilization is a common method of fertilization in the case of both humans and animals. Although this method has developed very rapidly, there are still many unanswered questions about the optimal characteristics that oocytes should have for performing this procedure. The object of this study was to describe the correlation between the diameter and size of swine oocytes in relation to pubescence. The research was conducted on ovaries of piglets (n = 65) and adult swine (n = 69). Each oocyte was examined with and without the zona pellucida. The analysis showed that the average size of the …

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Comparative Evaluation Of Factors Affecting Hemagglutinatingactivity Of Avian Influenza (H9) Virus, Asfa Rasool, Sameera Akhtar, Khushi Muhammad, Masood Rabbani, Aftab Ahmed Anjum, Javed Muhammad, Arfan Ahmad, Ali Ahmad Sheikh, Fakhra Liaqat, Fariha Akhtar, Rabia Tahir Jan 2016

Comparative Evaluation Of Factors Affecting Hemagglutinatingactivity Of Avian Influenza (H9) Virus, Asfa Rasool, Sameera Akhtar, Khushi Muhammad, Masood Rabbani, Aftab Ahmed Anjum, Javed Muhammad, Arfan Ahmad, Ali Ahmad Sheikh, Fakhra Liaqat, Fariha Akhtar, Rabia Tahir

Turkish Journal of Veterinary & Animal Sciences

Influenza virus H9 was standardized for hemagglutination assay using different factors such as red blood cell (RBC) types, concentrations, diluent types, and storage times. Avian influenza virus H9 was grown in embryonated chicken eggs and confirmed by spot agglutination. A significant (P < 0.05) difference with the highest mean titer (9.00 ± 0.00) was observed using RBCs from different species. Nonsignificant differences (P > 0.05) were found between human blood type O, chicken, and dog RBCs, as well as among rabbit, pigeon, sheep, and parrot. The highest titer (9.00 ± 0.00) with a nonsignificant difference was found using virus stored at 4 °C and -20 °C while 37 °C showed the lowest significant mean hemagglutinin (HA) titer (11.08 ± 188.21). Nonsignificant differences …

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Comparison Of Hematological Parameters Of Brucella-Seronegative Naturallyinfected Cattle With Bvdv, Bhv-1, And Bhv-4, Muhammet Eren Aslan, Ahmet Kürşat Azkur, Emel Biyikli, Serkal Gazyağci, İbrahi̇m Sözdutmaz Jan 2016

Comparison Of Hematological Parameters Of Brucella-Seronegative Naturallyinfected Cattle With Bvdv, Bhv-1, And Bhv-4, Muhammet Eren Aslan, Ahmet Kürşat Azkur, Emel Biyikli, Serkal Gazyağci, İbrahi̇m Sözdutmaz

Turkish Journal of Veterinary & Animal Sciences

Abortion, neonatal deaths, and infertility cause important production losses in the livestock industry. Many viral infections are responsible for changes in hematological values of animals. This study aimed to find whether there is any difference in blood parameters of cattle that are naturally infected with BVDV, BHV-1, and BHV-4 and Brucella-seronegative. For this purpose, 401 whole-blood samples collected from cattle were analyzed by ELISA and PCR/RT-PCR to detect specific antibodies and antigens for BVDV, BHV-1, and BHV-4. Hematological analysis and results were compared between naturally infected BVDV, BHV-1, and BHV-4 groups and a control group. Results showed a statistically significant …

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Subarachnoid Transplantation Of Autologous Neurogenically Induced Bone Marrow Derived Mesenchymal Stem Cells For The Treatment Of Fibrocartilaginous Embolic Myelopathy In Two Dogs, Pinar Can, Ömer Beşalti, Eylül Akpinar, Zeynep Aktaş, Ayşe Eser Elçi̇n, Yaşar Murat Elçi̇n Jan 2016

Subarachnoid Transplantation Of Autologous Neurogenically Induced Bone Marrow Derived Mesenchymal Stem Cells For The Treatment Of Fibrocartilaginous Embolic Myelopathy In Two Dogs, Pinar Can, Ömer Beşalti, Eylül Akpinar, Zeynep Aktaş, Ayşe Eser Elçi̇n, Yaşar Murat Elçi̇n

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the effects of autologous neurogenically induced bone marrow-derived mesenchymal stem cells (NIBM-MSCs) in two dogs suspected to have fibrocartilaginous embolic myelopathy (FCEM). Both dogs were paraplegic without deep pain perception (DPP) and tentatively diagnosed by MRI. Autologous NIBM-MSCs (5 × 106) were transplanted into the lumbar subarachnoid space two times with a 21-day interval for each patient. Based on a 21-month follow-up of the treated animals, the transplantation of NIBM-MSCs seems to be promising in subjects with FCEM lacking DPP.

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Estimates Of Genetic Parameters For Different Body Weights Andmuscle And Fat Depths Of Karayaka Lambs, Yüksel Aksoy, Zafer Ulutaş, Uğur Şen, Emre Şi̇ri̇n, Azi̇z Şahi̇n Jan 2016

Estimates Of Genetic Parameters For Different Body Weights Andmuscle And Fat Depths Of Karayaka Lambs, Yüksel Aksoy, Zafer Ulutaş, Uğur Şen, Emre Şi̇ri̇n, Azi̇z Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

In the current paper the direct additive and maternal genetic effects on birth, weaning (at 90 days of age), and scanning (at 20 weeks of age) weights and muscle and fat depths of the ribeye area in Karayaka lambs were investigated. Analyses were carried out by the restricted maximum likelihood approach, fitting 6 animal models with various combinations of direct and maternal effects. The best model was chosen after testing for improvement in the log-likelihood values. Direct heritability (h2d) for all traits decreased when maternal genetic effects were included in the models. The maternal heritability (h2m) ranged from 0.15 to …

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Evaluation Of Tear And Serum Trace Elements (Copper, Selenium, And Cobalt) In Sheep, Duygu Tarhan, Si̇nem Ülgen, Fatma Ateş Alkan, Di̇lek Olgun Erdi̇kmen, Çağla Parkan Yaramiş, Mehmet Erman Or, Ümi̇t Bora Barutçu Jan 2016

Evaluation Of Tear And Serum Trace Elements (Copper, Selenium, And Cobalt) In Sheep, Duygu Tarhan, Si̇nem Ülgen, Fatma Ateş Alkan, Di̇lek Olgun Erdi̇kmen, Çağla Parkan Yaramiş, Mehmet Erman Or, Ümi̇t Bora Barutçu

Turkish Journal of Veterinary & Animal Sciences

Metabolic diseases such as enzootic ataxia, white muscle disease, and white liver disease are among the most important problems of livestock for sheep. The aim of this study was to analyze whether tears can be used as an alternative diagnostic method in determination of diseases that result from lack or excess of copper, selenium, and cobalt. Samples of serum and tears taken from 45 healthy sheep of the Kivircik breed aged between 2 and 4 years were analyzed. The concentrations of copper, selenium, and cobalt were measured in serum and tears as 118.0 ± 36.0, 58.0 ± 15.0, and 0.5 …

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Detection Of Helicobacter Heilmannii Type Ii And Helicobacter Pylori Indogs And Their Role In The Development Of Gastritis, Si̇nem Ülgen, Sevgi̇ Ergi̇n, Gülbi̇n Şennazli, Utku Bakirel Jan 2016

Detection Of Helicobacter Heilmannii Type Ii And Helicobacter Pylori Indogs And Their Role In The Development Of Gastritis, Si̇nem Ülgen, Sevgi̇ Ergi̇n, Gülbi̇n Şennazli, Utku Bakirel

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to detect the presence of Helicobacter pylori and Helicobacter heilmannii type II in dogs and evaluate the role of Helicobacter spp. İn chronic gastritis. Gastroscopy was performed in a total of 40 dogs (22 symptomatic and 18 asymptomatic) admitted to the small animal clinic of İstanbul University. In this study, the density of spiral bacteria and the degree and type of gastritis by histopathology were determined. Rapid urease tests were performed on gastric biopsy samples, and the species identity was determined using nested polymerase chain reaction to assess for the presence of H. Heilmannii …

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Investigation Of Serum Testosterone Level, Scrotal Circumference, Body Mass,Semen Characteristics, And Their Correlations In Developing Mis Lambs, Nevena Maksimovic, Slavca Hristov, Branislav Stankovic, Milan P. Petrovic, Cvijan Mekic, Dragana Ruzic-Muslic, Violeta Caro-Petrovic Jan 2016

Investigation Of Serum Testosterone Level, Scrotal Circumference, Body Mass,Semen Characteristics, And Their Correlations In Developing Mis Lambs, Nevena Maksimovic, Slavca Hristov, Branislav Stankovic, Milan P. Petrovic, Cvijan Mekic, Dragana Ruzic-Muslic, Violeta Caro-Petrovic

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate changes of serum testosterone, scrotal circumference, body weight, and semen characteristics in a Meat Institute Sheep (MIS) population during their developing period (between 3 and 17 months). Correlative relationships among parameters were also tested. Body weight and scrotal circumference were measured monthly, blood samples for testosterone levels were taken bimonthly (3-17 months), and semen samples were collected by an electroejaculator between the ages of 9 and 17 months. The analysis showed that the average serum testosterone levels ranged from 1.83 to 13.28 ng/mL and significantly depended on the age of the lambs …

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Chemical And Tissue Composition Of Meat From Carcass Cuts Of Localgoats Affected By Different Feeding In Tunisian Arid Lands, Naziha Ayeb, Asma Ghrab, Ahmed Barmat, Touhami Khorchani Jan 2016

Chemical And Tissue Composition Of Meat From Carcass Cuts Of Localgoats Affected By Different Feeding In Tunisian Arid Lands, Naziha Ayeb, Asma Ghrab, Ahmed Barmat, Touhami Khorchani

Turkish Journal of Veterinary & Animal Sciences

An experiment was conducted to find out the nutritive value of different cuts of goat carcass. Meat samples were obtained from goat kids in Tunisia. Twenty-six indigenous kids were divided into three groups: oat hay (control), dried olive leaves + dried Stipa tenacissima, and grass hay. The cuts were the shoulder, chest, and leg of each goat carcass. Tissue compositions of different cuts and meat physicochemical parameters were determined. Percentage of muscle was higher in shoulders (61.57%) and legs (58.89%). The percentage of fat was higher in the chest (24.74%). Percentage of bone was higher in legs and shoulders. The …

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Purification Of Avian Influenza Virus (H9n2) From Allantoic Fluidby Size-Exclusion Chromatography, Ali Nazari Shirvan, Maedeh Samianifard, Naser Ghodsian Jan 2016

Purification Of Avian Influenza Virus (H9n2) From Allantoic Fluidby Size-Exclusion Chromatography, Ali Nazari Shirvan, Maedeh Samianifard, Naser Ghodsian

Turkish Journal of Veterinary & Animal Sciences

Avian influenza viruses represent a rising threat of pandemic influenza. Allantoic fluid including influenza virus H9N2 was run on downstream processing to purify the virus. To do this, allantoic fluid was clarified and concentrated. It was applied to size exclusion chromatography. Different parameters such as ovalbumin, protein content, and hemagglutinin activity were determined to confirm the purity. The results may suggest that this method is quite efficient for achieving a purified H9N2 influenza virus and further reveal that size-exclusion chromatography is a practical method for purifying avian influenza viruses. In conclusion, this procedure can be used in vaccine production and …

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Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha Jan 2016

Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha

Turkish Journal of Veterinary & Animal Sciences

Merkel cell tumors, a type of cutaneous round-cell tumors, are rarely observed in dogs and cats. This tumor type originates from cutaneous neuroendocrine cells. In the present study, the morphology of canine and feline Merkel cell tumors and their immunoexpression of neuron-specific enolase, cytokeratin, chromogranin A, Ki67, and metallothionein were evaluated using histopathological and immunohistochemical methods. The canine Merkel cell tumor displayed a diffuse morphological pattern, whereas the feline tumor displayed a solid morphological pattern. Both tumors were positive for neuron-specific enolase and cytokeratin, but negative for chromogranin A. The Ki67 indexes in the canine and feline tumors were 7% …

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Effects Of Mycobacterium Phlei On Intestinal Microecology Andserum Immunological Parameters Of Weaned Piglets, Jin-Feng Zhong, Xiao-Qing Zhang, Wei-Gao Wu, Wei Tu, Zhen-Xiang Liu, Re-Jun Fang Jan 2016

Effects Of Mycobacterium Phlei On Intestinal Microecology Andserum Immunological Parameters Of Weaned Piglets, Jin-Feng Zhong, Xiao-Qing Zhang, Wei-Gao Wu, Wei Tu, Zhen-Xiang Liu, Re-Jun Fang

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of Mycobacterium phlei in diets (Experiment 1) and investigate the mode of action on the intestinal microecology of weaned piglets (Experiments 1 and 2 ). In Experiment 1, a total of 96 commercial cross-bred pigs [(Duroc × Yorkshire) × Landrace], weaned at 21 days, were assigned to two treatments in a completely randomized design with a single factorial arrangement of 40 days of supplementation (nonsupplemented vs. supplemented with 0.4% heat-killed M. Phlei). The results showed that M. Phlei has the capability to improve the intestinal microecology of weaned piglets. The …

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Ultrasonographic Features Of Medial Iliac And Jejunal Lymph Nodesin Apparently Healthy Dogs, Shriram Ganesan, Jitender Mohindroo, Pallavi Verma, Narinder Singh Saini Jan 2016

Ultrasonographic Features Of Medial Iliac And Jejunal Lymph Nodesin Apparently Healthy Dogs, Shriram Ganesan, Jitender Mohindroo, Pallavi Verma, Narinder Singh Saini

Turkish Journal of Veterinary & Animal Sciences

Ultrasonographic examination of medial iliac and jejunal lymph nodes were performed in 36 dogs presented to the Department of Surgery and Radiology for spaying and castration. Ultrasonographic features such as length, height in longitudinal plane, shape, ease of scanning, echotexture, and echogenicity were recorded. The medial iliac and jejunal lymph nodes were scanned consistently in all 36 dogs. However, the presence of gas in the intestines affected the ease of scanning of the lymph nodes. The majority of these lymph nodes were easy to scan, and had homogeneous and hypoechoic echogenicity. The dogs were grouped based on body weight and …

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Seroepidemiological Survey Of Bovine Tick-Borne Infections In Theblack Sea Region Of Turkey, Mustafa Açici, Cenk Soner Bölükbaş, Gökmen Zafer Pekmezci̇, Ali̇ Tümay Gürler, Şi̇nasi̇ Umur, Kadri̇ Zafer Karaer, Ayşe Çakmak, Ayşe Serpi̇l Nalbantoğlu, Cevat Ni̇sbet Jan 2016

Seroepidemiological Survey Of Bovine Tick-Borne Infections In Theblack Sea Region Of Turkey, Mustafa Açici, Cenk Soner Bölükbaş, Gökmen Zafer Pekmezci̇, Ali̇ Tümay Gürler, Şi̇nasi̇ Umur, Kadri̇ Zafer Karaer, Ayşe Çakmak, Ayşe Serpi̇l Nalbantoğlu, Cevat Ni̇sbet

Turkish Journal of Veterinary & Animal Sciences

Babesiosis, theileriosis, and anaplasmosis are the tick-borne diseases of cattle in most of the tropical areas and Turkey. A total of 270 cattle were randomly selected from 27 villages and 77 farms in the Black Sea Region of Turkey for the determination of infections by Babesia bigemina and Anaplasma marginale by using the indirect fluorescent antibody (IFA) test and the competitive enzyme-linked immunosorbent assay (cELISA). Of those, 242 and 256 sera were also analyzed for antibodies against Theileria annulata and Babesia bovis infections using the IFA test, respectively, during the period of June 2006 to June 2007. Of the 256 …

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Lipid And Protein Metabolism Along With Oxidative Status Of Follicular Fluidthroughout The Estrous Cycle In Anatolian Water Buffalo, Oktay Yilmaz, Jerzy Jan Jaroszewski, Azi̇z Bülbül, Mehmet Uçar Jan 2016

Lipid And Protein Metabolism Along With Oxidative Status Of Follicular Fluidthroughout The Estrous Cycle In Anatolian Water Buffalo, Oktay Yilmaz, Jerzy Jan Jaroszewski, Azi̇z Bülbül, Mehmet Uçar

Turkish Journal of Veterinary & Animal Sciences

This study was performed to determine the concentrations of creatinine (CRE), high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood urea nitrogen (BUN), nitric oxide (NO), and malondialdehyde (MDA) in follicular fluid (FF) in relation to follicular size and/or stage of the estrous cycle [early I luteal (n = 14), early II luteal (n = 16), late luteal (n = 16), and follicular (n = 16) phases] in Anatolian water buffaloes. The concentrations of CRE (P < 0.001), HDL (P < 0.001), LDL (P < 0.05) BUN (P < 0.001), and NO (P < 0.01) in FF of large follicles (LFs, ?10 to

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Effects Of Ultrasonication On Damaged Spermatozoa And Mitochondrial Activity Rate, Pinar Peker Akalin, Nuri̇ Başpinar, Kenan Çoyan, Mustafa Numan Bucak, Şükrü Güngör, Caner Öztürk Jan 2016

Effects Of Ultrasonication On Damaged Spermatozoa And Mitochondrial Activity Rate, Pinar Peker Akalin, Nuri̇ Başpinar, Kenan Çoyan, Mustafa Numan Bucak, Şükrü Güngör, Caner Öztürk

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams (n = 3) and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6- and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased (P < 0.05) while mitochondrial activity rates decreased (P < 0.05). In relation with sonication duration time, total antioxidant potential levels increased (P < 0.05) in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate.

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Atopic Dog Skin Shows Decrease Of Claudin-1 But Increase Of Atopic Signature Cytokines, Sirin Theerawatanasirikul, Pakawadee Pongket, Lawan Larsuprom, Amornrate Sastravaha Jan 2016

Atopic Dog Skin Shows Decrease Of Claudin-1 But Increase Of Atopic Signature Cytokines, Sirin Theerawatanasirikul, Pakawadee Pongket, Lawan Larsuprom, Amornrate Sastravaha

Turkish Journal of Veterinary & Animal Sciences

Canine atopic dermatitis is a common allergic skin disease in dogs caused by defects in immunological and epidermal barriers. Claudin-1, a protein present in tight junctions, is associated with many skin conditions. Our aim was to investigate expression levels of claudin-1 and atopic signature cytokines in atopic and normal dog skins. The affected skin showed significantly decreased intensity and epidermal distribution of claudin-1 compared to the normal control skin (P < 0.05). The CD3+/CD4+ and CD3+/CD8+ T cell subsets, eosinophils, and neutrophils infiltrated the affected epidermis and dermis. The CD3+/CD4+ T cell subsets in lesional skin were significantly higher than CD3+/CD8+ T cell subsets. CLDN-1 mRNA expression was markedly downregulated in the lesional skin, while IL-17A was upregulated in both lesional and nonlesional skin. IL-4 and IL-31 mRNA was upregulated in the lesional skin, and TNF-α mRNA was significantly upregulated in the nonlesional skin (P < 0.01 and P < 0.05, respectively). Correlation-based hierarchical clustering showed that CLDN-1 expression was closely clustered with IL-31 but loosely clustered with L-4, IL-10, IL-13, and IL-17A. We suggest that the alteration of claudin-1 in atopic dogs may disrupt the skin barrier and allow an influx of allergens, inciting inflammatory cytokine responses.

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Polymorphism Of Exon 2 Of Dio2 Gene And Its Association Withseasonal Reproduction In Sheep, Jianning He, Dongwei Huang, Ran Di, Jinxin Wang, Mingxing Chu, Qiuyue Liu, Wenping Hu, Xiangyu Wang, Zhangyuan Pan Jan 2016

Polymorphism Of Exon 2 Of Dio2 Gene And Its Association Withseasonal Reproduction In Sheep, Jianning He, Dongwei Huang, Ran Di, Jinxin Wang, Mingxing Chu, Qiuyue Liu, Wenping Hu, Xiangyu Wang, Zhangyuan Pan

Turkish Journal of Veterinary & Animal Sciences

DIO2 was studied as a candidate gene for reproduction in sheep. Three pairs of primers were designed to detect single nucleotide polymorphisms (SNPs) of exon 2 of DIO2 in nonseasonal and seasonal reproduction breeds. The polymorphisms were detected using SeqMan II and analyzed by PCR-RFLP. The sheep DIO2 gene was cloned and its mutations were detected in five sheep breeds whose reproductive seasonality and litter sizes were different. Association analyses were performed to reveal the relationships between SNPs and reproductive seasonality as well as litter size in sheep. The results showed that a 1041-bp DNA fragment of the sheep DIO2 …

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Comparative Study Between Formalin-Killed Vaccine And Developed Gammairradiation Vaccine Against Mannheimia Haemolytica In Rabbits, Sahar Ahmed, Basem Ahmed, Ghada Mahmoud, Waleed Nemr, Emam Abdel Rahim Jan 2016

Comparative Study Between Formalin-Killed Vaccine And Developed Gammairradiation Vaccine Against Mannheimia Haemolytica In Rabbits, Sahar Ahmed, Basem Ahmed, Ghada Mahmoud, Waleed Nemr, Emam Abdel Rahim

Turkish Journal of Veterinary & Animal Sciences

Mannheimia haemolytica is responsible for considerable economic losses to sheep, goats, and cattle and other livestock industries in Egypt. This study aimed to evaluate the effectiveness of a newly developed gamma irradiation vaccine against Mannheimia haemolytica in comparison to a formalin-killed vaccine. Three groups of rabbits were used in this study. Group 1 animals were inoculated with 4 × 109 bacterial cells per dose of the formalin-killed vaccine. Group 2 was inoculated with 2 × 109 bacterial cells per dose of gamma-irradiated vaccine. Group 3 (control group) was injected with 2 mL of sterile PBS. The vaccines were injected subcutaneously …

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