Veterinary Medicine Commons^™

The Treatment Of Distal Metacarpus Fracture With Locking Compression Plate In Calves*, Ali̇ Belge, İbrahi̇m Akin, Ali̇ Gülaydin, Muhammed Fati̇h Yazici

Turkish Journal of Veterinary & Animal Sciences

In this study, we aimed to evaluate the effect of locking compression plates for the treatment of distal metacarpal fractures in calves. The metacarpus was stabilized using a stainless steel locking compression plate with fully threaded locking head screws. The calves started weight-bearing partially after the first postoperative day and resumed complete weight-bearing after day 10. Callus formation was obtained at the end of the 3rd week and completed at the end of the 8th week, and complete healing was seen at the 12th week in radiographs. The limbs were in good alignment, the calves were fully weight-bearing, and client …

Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial

Turkish Journal of Veterinary & Animal Sciences

The activity of enzymes that digest carbohydrates at various points in the gastrointestinal tract of sheep was examined. In addition, weight and contents of digestive tract segments were examined. The experiment was performed on six adult ewes fed an 85% hay diet. The morphometric measurements revealed that the alimentary tract constituted 32% of the sheep's body mass and wet rumen contents constituted 70% of wet mass of the total digestive tract content. The protozoal population in the rumen varied from 37.2 × 104 to 54.0 × 104/mL rumen fluid. In the examined samples, ciliates were identified as belonging to the …

The Effect Of Seasonal Variations On The Occurrence Of Certain Mycotoxins Inconcentrate Feeds For Cattle Collected From Some Provinces In Turkey, Hüsametti̇n Eki̇ci̇, Ebru Yildirim, Ender Yarsan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of seasonal variations on the occurrence of total aflatoxin (AFTotal), aflatoxin B1 (AFB1), ochratoxin A (OTA), and total fumonisin (Ftotal) in the commercially mixed ruminant feed obtained from feed mills located in Ankara, Kırıkkale, Çankırı, Çorum, and Kırşehir provinces using the enzyme-linked immunoassay (ELISA) method. To represent the annual production, 22 samples were collected every quarter over 1 year (a total of 88 samples) starting in September 2012. AFTotal and AFB1 were detected in 72 samples (81.81%), OTA in 84 samples (95.45%), and Ftotal in 83 samples (94.31%). When seasonal …

Performance Of Broilers Fed Raw Or Fermented And Redried Wheat, Barley, And Oat Grains, Sulhatti̇n Yaşar, Muhammed Saci̇d Gök, Yavuz Gürbüz

Turkish Journal of Veterinary & Animal Sciences

The efficacy of diets containing 40%-50% wheat, barley, or oat grains, fermented by a solid-state fermentation process, on bird performance, carcass yield, digestive organ development, and intestinal viscosity was tested. Compared with broiler chicks fed unfermented grains, those fed fermented oats had increased feed intake, and treatment with fermented wheat and barley improved the feed conversion ratio at days 21 and 42. Treatment with fermented barley and oats resulted in increased body weight at day 42. Treatment with fermented wheat and oats increased the percentage of carcass yield. Birds fed unfermented grains had highly viscous digesta, not seen among birds …

The Effects Of Probiotics, Organic Acid, And A Medicinal Plant On The Immune Systemand Gastrointestinal Microflora In Broilers Challenged With Campylobacter Jejuni, Hossein Ebrahimi, Shaban Rahimi, Pejvak Khaki, Jesse L. Grimes, Sophia Kathariou

Turkish Journal of Veterinary & Animal Sciences

Campylobacter jejuni, a zoonotic bacterial pathogen with worldwide distribution, infects about 400 million humans in the world annually. In order to reduce C. Jejuni colonization in the gastrointestinal tracts of broilers and make chickens less susceptible to colonization, four commercial products based on organic acid, probiotics, and medicinal plants were used. In this experiment, 210 one-day-old male broiler chicks (Ross 308) were assigned to 7 treatment groups randomly with 3 replications and 10 birds in each pen. Birds were challenged on day 21 by 1 mL of 6 × 107 CFU/mL C. Jejuni live suspension and samples were collected on …

Application Of Herbs And Propolis In Rabbits With Chronic Diarrhea, Robert Kupczynski, Tomasz Piasecki, Michal Bednarski, Kinga Spitalniak, Anna Budny-Walczak

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effect of ethanolic extract of propolis (EEP) and an herbal mixture (Rumex crispus, Potentilla anserina, and Polygonum aviculare) on diarrhea course, hematological and biochemical parameters, and acid-base balance of rabbits. Twenty-four meat rabbits (Hyplus) were divided into four groups of eight animals. The rabbits were randomly divided into the following groups: HR - healthy rabbits, DR - rabbits with diarrhea symptoms, H - treated with 10% herbal extract, and EEP - treated with 10% ethanolic extract of propolis at 4 mL/L. The extracts were supplemented for 10 days into the drinking …

Influence Of Flaxseed Combined With Thyme, Rosemary, And Sage Leaves Asfodder Additives On Antioxidant Status In The Liver Of Japanese Quail, Radoslaw Drozd, Artur Rybarczyk, Agata Wasak, Malgorzata Jakubowska, Kinga Rybak, Magdalena Skolmowska

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the influence of a combination of plant supplements with antioxidant properties on the total antioxidant status measured by FRAP and the activity of selected antioxidant enzymes in the livers of Japanese quails. The birds were given feed with the 4% addition of flaxseed combined with a 1% admixture of dried thyme, rosemary, or sage leaves, respectively. The results showed an increased value of the antioxidant potential in the livers of quails fed with a diet that contained the combination of flaxseed and thyme, compared to the control diet and the diet that …

Comparative Efficacy Of Antioxidant Retinol, Melatonin, And Zinc During In Vitro Maturation Of Bovine Oocytes Under Induced Heat Stress, Jafrin Ara Ahmed, Devojyoti Dutta, Nawab Nashiruddullah

Turkish Journal of Veterinary & Animal Sciences

Both A and B grade cumulus-oocyte complexes (COCs) aspirated from cattle ovaries at slaughter were matured in vitro under normal (38.5 °C) and elevated temperatures (41 °C). Maturation competence based on cumulus expansion, COC diameter, and nuclear maturation were compared with and without antioxidant supplementation incorporating 7.5 µM retinol, 1 nM melatonin, and 1.5 µg/mL zinc chloride in an oocyte maturation medium. Heat stress significantly reduced cumulus expansion by approximately 20%, while only retinol could bring a significant (P ≤ 0.05) increase. Heat stress also decreased expansion of A (approximately 50%) and B (approximately 40%) grade COC diameter. All antioxidants …

Mosquito Host Choices On Livestock Amplifiers Of Rift Valley Fever Virus In Kenya, David P. Tchouassi, Robinson O. Okiro, Rosemary Sang, Lee W. Cohnstaedt, D. Scott Mcvey, Baldwyn Torto

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Animal hosts may vary in their attraction and acceptability as components of the host location process for assessing preference, and biting rates of vectors and risk of exposure to pathogens. However, these parameters remain poorly understood for mosquito vectors of the Rift Valley fever (RVF), an arboviral disease, and for a community of mosquitoes.

Methods: Using three known livestock amplifiers of RVF virus including sheep, goat and cattle as bait in enclosure traps, we investigated the host-feeding patterns for a community of mosquitoes in Naivasha, an endemic area of Rift Valley fever (RVF), in a longitudinal study for six …

Analysis Of Mycobacterium Avium Subsp. Paratuberculosis Mutant Libraries Reveals Loci-Dependent Transposition Biases And Strategies For Novel Mutant Discovery, Govardhan Rathnaiah, John Bannantine, Darrell O. Bayles, Denise K. Zinniel, Judith R. Stabel, Yrjö T. Gröhn, Raúl G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium subsp. paratuberculosis (MAP), the aetiological agent of Johne’s disease, is one of the most important bacterial pathogens in ruminants. A thorough understanding of MAP pathogenesis is needed to develop new vaccines and diagnostic tests. The generation of comprehensive random transposon mutant libraries is a fundamental genetic technology to determine the role of genes in physiology and pathogenesis. In this study, whole MAP genome analysis compared the insertion sites for the mycobacterial transposon Tn5367 derived from the Mycobacterium smegmatis insertion sequence IS1096 and the mariner transposon MycoMarT7 carrying the Himar1 transposase. We determined that only MycoMarT7 provides …

A Synthetic Porcine Reproductive And Respiratory Syndrome Unprecedented Levels Of Heterologous Protection, Hiep Vu, Fangrui Ma, William W. Laegreid, Asit K. Pattnaik, David Steffen, Alan R. Doster, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

Current vaccines do not provide sufficient levels of protection against divergent porcine reproductive and respiratory syndrome virus (PRRSV) strains circulating in the field, mainly due to the substantial variation of the viral genome. We describe here a novel approach to generate a PRRSV vaccine candidate that could confer unprecedented levels of heterologous protection against divergent PRRSV isolates. Using a set of 59 non-redundant, full genome sequences of type-2 PRRSV, a consensus genome (designated as PRRSV-CON) was these 59 PRRSV full genome sequences, followed by selecting the most common nucleotide found at each position of the alignment. Next, the synthetic PRRSV-CON …

Overview Of Rhabdo- And Filoviruses, Asit K. Pattnaik, Michael A. Whitt

School of Veterinary and Biomedical Sciences: Faculty Publications

Enveloped viruses with a negative-sense, single-stranded monopartite RNA genome have been classified into the order Mononegavirales. Five families of viruses that constitute the order are: Rhabdoviridae, Filoviridae, Paramyxoviridae, Bornaviridae and Nyamiviridae. Members of these families possess a helical nucleocapsid core containing the viral genome and a host-derived lipid envelope containing viral proteins. This introductory chapter provides a brief overview of the Rhabdoviridae and the Filoviridae, the two families of viruses that are the subject of this book. Many members of these two families are highly significant human and animal pathogens. The rationale and goal of the book is to …

On And Off Retinal Ganglion Cells Differentially Regulate Serotonergic And Gabaergic Activity In The Dorsal Raphe Nucleus, Ting Zhang, Lu Huang, Li Zhang, Minjie Tan, Mingliang Pu, Gary E. Pickard, Kwok -Fai So, Chaoran Ren

School of Veterinary and Biomedical Sciences: Faculty Publications

The dorsal raphe nucleus (DRN), the major source of serotonergic input to the forebrain, receives excitatory input from the retina that can modulate serotonin levels and depressive-like behavior. In the Mongolian gerbil, retinal ganglion cells (RGCs) with alpha-like morphological and Y-like physiological properties innervate the DRN with ON DRN-projecting RGCs out numbering OFF DRN-projecting RGCs. The DRN neurons targeted by ON and OFF RGCs are unknown. To explore retino-raphe anatomical organization, retinal afferents labeled with Cholera toxin B were examined for association with the postsynaptic protein PSD-95. Synaptic associations between retinal afferents and DRN serotonergic and GABAergic neurons were observed. …

Host Cell Functions In Vesicular Stomatitis Virus Replication, Phat X. Dinh, Anshuman Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Vesicular stomatitis virus (VSV), the prototypic rhabdovirus, has been used as an excellent paradigm for understanding the mechanisms of virus replication, pathogenesis, host response to virus infection and also for myriads of studies on cellular and molecular biology. Biochemical studies as well as high-throughput genomics, proteomics, and chemical approaches have revealed a plethora of cellular factors and pathways that regulate replication of VSV. These factors include those that support virus replication and also those that restrict its replication. This chapter discusses the role(s) of many of these host cell factors and pathways involved in VSV replication. Although mechanistic understanding of …

Resolving Bovine Viral Diarrhea Virus Subtypes From Persistently Infected U.S. Beef Calves With Complete Genome Sequence, Aspen M. Workman, Michael P. Heaton, Gregory P. Harhay, Timothy P.L. Smith, Dale M. Grotelueschen, David Sjeklocha, Bruce Brodersen, Jessica L. Petersen, Carol G. Chitko-Mckown

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine viral diarrhea virus (BVDV) is classified into 2 genotypes, BVDV-1 and BVDV-2, each of which contains distinct subtypes with genetic and antigenic variation. To effectively control BVDV by vaccination, it is important to know which subtypes of the virus are circulating and how their prevalence is changing over time. Accordingly, the purpose of our study was to estimate the current prevalence and diversity of BVDV subtypes from persistently infected (PI) beef calves in the central United States. Phylogenetic analysis of the 5′-UTR (5′ untranslated region) for 119 virus strains revealed that a majority (82%) belonged to genotype 1b, and …

New Tools To Convert Bacterial Artificial Chromosomes To A Self-Excising Design And Their Application To A Herpes Simplex Virus Type 1 Infectious Clone, Alexsia L. Richards, Patricia J. Sollars, Gregory A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Infectious clones are fundamental tools for the study of many viruses, allowing for efficient mutagenesis and reproducible production of genetically-defined strains. For the large dsDNA genomes of the herpesviridae, bacterial artificial chromosomes have become the cloning vector of choice due to their capacity to house full-length herpesvirus genomes as single contiguous inserts. Furthermore, while maintained as plasmids in Escherichia coli, the clones can be mutated using robust prokaryotic recombination systems. An important consideration in the design of these clones is the means by which the vector backbone is removed from the virus genome upon delivery into mammalian cells. …

The Mechanistic Basis Of Hemoglobin Adaptation In The High-Flying Barheaded Goose: Insights From Ancestral Protein Resurrection, Chandrasekhar Natarajan, Amit Kumar, Hideaki Moriyama, Roy E. Weber, Angela Fago, Jay F. Storz

School of Veterinary and Biomedical Sciences: Faculty Publications

The bar-headed goose (‘BHG’, Anser indicus) is renowned for its trans-Himalayan migratory flights, and the elevated hemoglobin (Hb)-O2 affinity of this species is thought to make a key contribution to its capacity for powered flight at elevations of ~9000 m. Here we revisit the molecular basis of this text-book example of biochemical adaptation. Previous hypotheses about the molecular basis of the evolved increase in Hb-O2 affinity were tested by engineering BHGspecific mutations into recombinant human Hb. This approach can provide important insights, but one problem with such ‘horizontal’ comparisons – swapping residues between proteins of contemporary species – is that …

Maporal Hantavirus Causes Mild Pathology In Deer Mice (Peromyscus Maniculatus), Amanda Mcguire, Kaitlyn Miedema, Joseph R. Fauver, Amber Rico, Tawfik Aboellail, Sandra L. Quackenbush, Ann Hawkinson, Tony Schountz

School of Veterinary and Biomedical Sciences: Faculty Publications

Rodent-borne hantaviruses can cause two human diseases with many pathological similarities: hantavirus cardiopulmonary syndrome (HCPS) in the western hemisphere and hemorrhagic fever with renal syndrome in the eastern hemisphere. Each virus is hosted by specific reservoir species without conspicuous disease. HCPS-causing hantaviruses require animal biosafety level-4 (ABSL-4) containment, which substantially limits experimental research of interactions between the viruses and their reservoir hosts. Maporal virus (MAPV) is a South American hantavirus not known to cause disease in humans, thus it can be manipulated under ABSL-3 conditions. The aim of this study was to develop an ABSL-3 hantavirus infection model using the …

Localization Of Cd8 T Cell Epitope Within Cardiac Myosin Heavy Chain-Α334-352 That Induces Autoimmune Myocarditis In A/J Mice, Chandirasegara Massilamany, Arunakumar Gangaplara, Rakesh H. Basavalingappa, Rajkumar A. Rajasekaran, Vahid Khalilzad-Sharghi, Zhongji Han, Shadi F. Othman, David Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Background—Cardiac myosin heavy chain-α (Myhc), an intracellular protein expressed in the cardiomyocytes, has been identified as a major autoantigen in cardiac autoimmunity. In our studies with Myhc_334-352-induced experimental autoimmune myocarditis in A/J mice (H-2a), we discovered that Myhc_334-352, supposedly a CD4 T cell epitope, also induced antigen-specific CD8 T cells that transfer disease to naïve animals.

Methods and Results—In our efforts to identify the CD8 T cell determinants, we localized Myhc_338-348 within the full length-Myhc_334-352, leading to four key findings. (1) By acting as a dual epitope, Myhc_338-348 induces both …

Note On Ehrlichia Chaffeensis, Ehrlichia Ewingii, And “Borrelia Lonestari” Infection In Lone Star Ticks (Acari: Ixodidae), Nebraska, Usa, Amanda Maegli, J. Dustin Loy, Roberto Cortinas

School of Veterinary and Biomedical Sciences: Faculty Publications

The lone star tick, Amblyomma americanum (L.) (Acari: Ixodidae), is established in southeastern Nebraska, yet the prevalence of tick-associated microorganisms is not known. An initial PCR-based analysis for Ehrlichia chaffeensis, Ehrlichia ewingii, and Borrelia infection in host-seeking adult ticks collected in southeast Nebraska was conducted. A total of 251 adult ticks collected in six sites in southeast Nebraska were tested. E. chaffeensis, E. ewingii, and Borrelia spp. were present, and the prevalence of each was approximately 1.6%. This study demonstrates that Ehrlichia spp. are present in Nebraska lone star tick populations.

Expression And Distribution Of Thiol-Regulating Enzyme Glutaredoxin 2 (Grx2) In Porcine Ocular Tissues, Bijaya Upadhyaya, Xiaoli Tian, Hongli Wu, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin2 (Grx2) is a mitochondrial isozyme of the cytosolic glutaredoxin1 (thioltransferase or TTase). Both belong to the large oxidoreductase family and play an important role in maintaining thiol/disulfide redox homeostasis in the cells. Grx2 is recently found in the lens where its activities of disulfide reductase and peroxidase, similar to TTase, can protect the lens against oxidative stress. Since other eye tissues are also highly sensitive to oxidative stress, and TTase’s distribution in the eye is known, we focused on this study by investigating the Grx2 distribution in the ocular tissues in comparison to the lens. Fresh porcine eyes were …

Autoimmunity In Picornavirus Infections, Chandirasegara Massilamany, Andreas Koenig, Jay Reddy, Sally Huber, Iwona Buskiewicz

School of Veterinary and Biomedical Sciences: Faculty Publications

Enteroviruses are small, non-enveloped, positive-sense singlestrand RNA viruses, and are ubiquitously found throughout the world. These viruses usually cause asymptomatic or mild febrile illnesses, but have a propensity to induce severe diseases including type 1 diabetes and pancreatitis, paralysis and neuroinflammatory disease, myocarditis, or hepatitis. This pathogenicity may result from induction of autoimmunity to organ-specific antigens. While enterovirus-triggered autoimmunity can arise from multiple mechanisms including antigenic mimicry and release of sequestered antigens, the recent demonstration of T cells expressing dual T cell receptors arising as a natural consequence of Theiler’s virus infection is the first demonstration of this autoimmune mechanism.

Genome-Wide Search For Host Association Factors During Ovine Progressive Pneumonia Virus Infection, Jesse Thompson, Fangrui Ma, Meghan Quinn, Shi-Hua Xiang

School of Veterinary and Biomedical Sciences: Faculty Publications

Ovine progressive pneumonia virus (OPPV) is an important virus that causes serious diseases in sheep and goats with a prevalence of 36% in the USA. Although OPPV was discovered more than half of a century ago, little is known about the infection and pathogenesis of this virus. In this report, we used RNA-seq technology to conduct a genome-wide probe for cellular factors that are associated with OPPV infection. A total of approximately 22,000 goat host genes were detected of which 657 were found to have been significantly up-regulated and 889 down-regulated at 12 hours post-infection. In addition to previously known …

From Crystal Structure Of Α-Conotoxin Gic In Complex With Ac-Achbp To Molecular Determinants Of Its High Selectivity For Α3Β2 Nachr, Bo Lin, Manyu Xu, Xiaopeng Zhu, Yong Wu, Xi Liu, Dongting Zhangsun, Yuanyan Hu, Shi-Hua Xiang, Igor E. Kasheverov, Victor I. Tsetlin, Xinquan Wang, Sulan Luo

School of Veterinary and Biomedical Sciences: Faculty Publications

Acetylcholine binding proteins (AChBPs) are unique spatial homologs of the ligand-binding domains of nicotinic acetylcholine receptors (nAChRs), and they reproduce some pharmacological properties of nAChRs. X-ray crystal structures of AСhBP in complex with α-conotoxins provide important insights into the interactions of α-conotoxins with distinct nAChR subtypes. Although considerable efforts have been made to understand why α-conotoxin GIC is strongly selective for α3β2 nAChR, this question has not yet been solved. Here we present the structure of α-conotoxin GIC in complex with Aplysia californica AChBP (Ac-AChBP) at a resolution of 2.1 Å. Based on this co-crystal structure complemented with molecular docking …

From Crystal Structure Of Α-Conotoxin Gic In Complex With Ac-Achbp To Molecular Determinants Of Its High Selectivity For Α3Β2 Nachr, Bo Lin, Manyu Xu, Xiaopeng Zhu, Yong Wu, Xi Liu, Dongting Zhangsun, Yuanyan Hu, Shi-Hua Xiang, Igor E. Kasheverov, Victor I. Tsetlin, Xianquan Wang, Sulan Luo

School of Veterinary and Biomedical Sciences: Faculty Publications

Acetylcholine binding proteins (AChBPs) are unique spatial homologs of the ligand-binding domains of nicotinic acetylcholine receptors (nAChRs), and they reproduce some pharmacological properties of nAChRs. X-ray crystal structures of AСhBP in complex with α-conotoxins provide important insights into the interactions of α-conotoxins with distinct nAChR subtypes. Although considerable efforts have been made to understand why α-conotoxin GIC is strongly selective for α3β2 nAChR, this question has not yet been solved. Here we present the structure of α-conotoxin GIC in complex with Aplysia californica AChBP (Ac-AChBP) at a resolution of 2.1 Å. Based on this co-crystal structure complemented with molecular docking …

Guidelines For The Use And Interpretation Of Assays For Monitoring Autophagy (3rd Edition), Daniel J. Klionsky, Rodrigo Franco, More Than 2,000 Co-Authors

School of Veterinary and Biomedical Sciences: Faculty Publications

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes.

For example, a key point that needs to be emphasized is that …

Echinococcus Multilocularis In Foxes And Raccoon Dogs: An Increasing Concern For Baltic Countries, Guna Bagrade, Gunita Deksne, Zanda Ozoliņa, Samantha Jane Howlett, Maria Interisano, Adriano Casulli, Edoardo Pozio

Harold W. Manter Laboratory of Parasitology: Library Materials

Background

In Europe, the life cycle of Echinococcus multilocularis is predominantly sylvatic, involving red foxes (Vulpes vulpes) as the main definitive hosts and rodents such as muskrats and arvicolids as intermediate hosts. The parasite is the etiological agent of human alveolar echinococcosis, a malignant zoonotic disease caused by the accidental ingestion of eggs shed by definitive hosts in their feces. The aims of this study were to investigate the prevalence of E. multilocularis in red foxes and raccoon dogs (Nyctereutes procyonoides) and to study the environmental factors favoring the perpetuation of the parasite in Latvia. …

Breaking The Silence: The Veterinarian’S Duty To Report, Martine Lachance

Animal Sentience

Animals, like children and disabled elders, are not only the subjects of abuse, but they are unable to report and protect themselves from it. Veterinarians, like human physicians, are often the ones to become aware of the abuse and the only ones in a position to report it when their human clients are unwilling to do so. This creates a conflict between professional confidentiality to the client and the duty to protect the victim and facilitate prosecution when the law has been broken. I accordingly recommend that veterinarian associations make reporting of abuse mandatory.

Veterinarians Need Support To Break The Silence, Anne Fawcett

Animal Sentience

The question of whether reporting animal abuse should be mandatory for veterinarians is an important one. Veterinarians have dual obligations, both to their animal patients and to their human clients. Mandatory reporting can only be supported in a context in which veterinary professionals are (a) trained to recognise the signs of animal abuse; (b) trained to elicit a history sensitively from clients who may themselves be victims of abuse or supported in doing so; (c) supported by appropriate, responsive authorities; (d) reasonably protected from legal recriminations.

The Science Of Sentience Is Reshaping How We Think About Animals, Debra Durham

Animal Sentience

Broom’s Sentience and Animal Welfare (2014) provides its readers with an excellent overview of the evolving role of sentience in our understanding animal welfare as well as insights into why sentience matters for practice and policy.