Veterinary Medicine Commons^™

Local Admixture Of Amplified And Diversified Secreted Pathogenesis Determinants Shapes Mosaic Toxoplasma Gondii Genomes, Hernan Lorenzi, Asis Khan, Michael S. Behnke, Sivaranjani Namasivayam, Lakshmipuram S. Swapna, Michalis Hadjithomas, Svetlana Karamycheva, Deborah Pinney, Brian P. Brunk, James W. Ajioka, Daniel Ajzenberg, John C. Boothroyd, Jon P. Boyle, Marie L. Dardé, Maria A. Diaz-Miranda, Jitender P. Dubey, Heather M. Fritz, Solange M. Gennari, Brian D. Gregory, Kami Kim, Jeroen P. J. Saeij, Chunlei Su, Michael W. White, Xing-Quan Zhu, Daniel K. Howe, Benjamin M. Rosenthal, Michael E. Grigg, John Parkinson, Liang Liu, Jessica C. Kissinger

Veterinary Science Faculty Publications

Toxoplasma gondii is among the most prevalent parasites worldwide, infecting many wild and domestic animals and causing zoonotic infections in humans. T. gondii differs substantially in its broad distribution from closely related parasites that typically have narrow, specialized host ranges. To elucidate the genetic basis for these differences, we compared the genomes of 62 globally distributed T. gondii isolates to several closely related coccidian parasites. Our findings reveal that tandem amplification and diversification of secretory pathogenesis determinants is the primary feature that distinguishes the closely related genomes of these biologically diverse parasites. We further show that the unusual population structure …

Noninvasive Assessment Of Cardiac Abnormalities In Experimental Autoimmune Myocarditis By Magnetic Resonance Microscopy Imaging In The Mouse, Chandirasegaran Massilamany, Vahid Khalilzad-Sharghi, Arunakumar Gangaplara, David Steffen, Shadi F. Othman, Jay Reddy

Jay Reddy Publications

Myocarditis is an inflammation of the myocardium, but only ~10% of those affected show clinical manifestations of the disease. To study the immune events of myocardial injuries, various mouse models of myocarditis have been widely used. This study involved experimental autoimmune myocarditis (EAM) induced with cardiac myosin heavy chain (Myhc)-α 334-352 in A/J mice; the affected animals develop lymphocytic myocarditis but with no apparent clinical signs. In this model, the utility of magnetic resonance microscopy (MRM) as a non-invasive modality to determine the cardiac structural and functional changes in animals immunized with Myhc-α 334-352 is shown. EAM and healthy mice …

Research Of The Effects Of Autologous Cancellous Bone Graft And Hyaluronic Acidon The Healing Of Bone Defects Experimentally Induced In Rabbits, Aydin Sağliyan, Mehmet Cengi̇z Han, Eni̇s Karabulut, Mustafa Özkaraca

Turkish Journal of Veterinary & Animal Sciences

The present study investigated the effect of autograft and hyaluronic acid (HA) in terms of clinical, radiological, and histopathological aspects on the healing of experimentally induced bone defects. The animals (n = 42) were assorted into 3 groups. After general anesthesia, a 3-mm standard defect was formed from the tibia of the experimental animals. The defects in Group I (n = 14) were left blank. Cancellous grafts in the proximal tibia were collected using a small curette to fill the defects in Group II (n = 14). The defects in Group III (n = 14) were filled by mixing with …

Growing South Dakota (Winter 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2015 South Dakota Agricultural Experiment Station Annual Report.

[Page] 2 Director's Message
[Page] 3 Concentrating On Cattle: Researcher Studying Mechanics To Improve Reproduction Efficiency In Beef Cattle
[Page] 5 At The Forefront: SDSU Diagnostic Lab Scientists Sequence Avian Influenza Genome
[Page] 6 Seeking Superior Wheat: Breeder Using Extensive Genome Testing To Improve Winter Wheat Cultivars
[Page] 8 Profiles In Leadership: Plant And Fungi Interactions May Offer New Plant Production Possibilities
[Page] 9 Targeting Emerging Diseases: Plant Pathologists Working To Better Manage Soybean & Sunflower Diseases
[Page] 10 Compelling New Crop: Carinata Offers Potential For Crop Diversity, …

Exploring Attitudes Toward Euthanasia Among Shelter Workers And Volunteers In Japan And The U.S., Maho Cavalier

HSU STUDENT THESES AND CAPSTONE PROJECTS

Conflicts between foreign and Japanese volunteers concerning decisions to euthanize animals that are in terminal condition were noted during anecdotal observation by this author at a Japanese animal rescue shelter. Thus, this research was undertaken to explore attitudes of shelter staff in Japan. As a point of comparison, U. S. shelter workers were also interviewed. Twenty current or former shelter workers (16 participants from Japan and 4 participants from the U.S.), ranging in age from 20 to 65, were interviewed. Face-to-face interviews were conducted with the Japanese participants; e-mail and/or Skype were used to conduct the interviews with the participants …

Dry Period Plane Of Energy: Effects On Glucose Tolerance In Transition Dairy Cows, S. Mann, F. A. Leal Yepes, M. Duplessis, J. J. Wakshlag, T. R. Overton, B. P. Cummings, D. V. Nydam

Physiology Collection

Overfeeding energy in the dry period can affect glucose metabolism and the energy balance of transition dairy cows with potential detrimental effects on the ability to successfully adapt to early lactation. The objectives of this study were to investigate the effect of different dry cow feeding strategies on glucose tolerance and on resting concentrations of blood glucose, glucagon, insulin, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) in the peripartum period. Cows entering second or greater lactation were enrolled at dry-off (57 d before expected parturition) into 1 of 3 treatment groups following a randomized block design: cows …

Body Condition Score And Plane Of Nutrition Prepartum Affect Adipose Tissue Transcriptome Regulators Of Metabolism And Inflammation In Grazing Dairy Cows During The Transition Period, M. Vailati-Riboni, M. Kanwal, O. Bulgari, S. Meier, N. V. Priest, C. R. Burke, J. K. Kay, S. Mcdougall, M. D. Mitchell, C. G. Walker, M. Crookenden, A. Heiser, J. R. Roche, J. J. Loor

Physiology Collection

Recent studies demonstrating a higher incidence of metabolic disorders after calving have challenged the management practice of increasing dietary energy density during the last ~3 wk prepartum. Despite our knowledge at the whole-animal level, the tissue-level mechanisms that are altered in response to feeding management prepartum remain unclear. Our hypothesis was that prepartum body condition score (BCS), in combination with feeding management, plays a central role in the peripartum changes associated with energy balance and inflammatory state. Twenty-eight mid-lactation grazing dairy cows of mixed age and breed were randomly allocated to 1 of 4 treatment groups in a 2 × …

Supplementation Effects Of Seleno-Compounds, Carnosic Acid, And Fish Oil Onconcentrations Of Fatty Acids, Tocopherols, Cholesterol, And Amino Acids In The Livers Of Lambs, Agnieszka Justyna Rozbicka-Wieczorek, Edyta Wiesyk, Katarzyna Agnieszka Krajewska-Bienias, Krzysztof Wereszka, Marian Czauderna

Turkish Journal of Veterinary & Animal Sciences

Lambs were fed a diet containing 3% rapeseed oil (RO) (the RO diet), a diet with 2% RO and 1% fish oil (FO) (the FO diet), or diets with combined additions of 2% RO, 1% FO, 0.1% carnosic acid (CA) (the CA diet), and 0.35 ppm Se as selenized yeast (SeY) (the CASeY diet) or selenate (SeVI) (the CASeVI diet). The CASeVI diet most effectively increased the body mass gain of lambs, while it most efficiently decreased the liver mass. The CASeVI diet most efficiently reduced the concentration of cholesterol and the lipid peroxidation in the liver. The CA diet …

Table Of Contents 2016 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

No abstract provided.

Cobalt Use And Regulation In Horseracing: A Review, K. Brewer, G. Maylin, C. K. Fenger, Thomas Tobin

Veterinary Science Faculty Publications

Cobalt, atomic weight 58.9, is a metallic element and environmental substance found in the animal in microgram quantities, predominantly as vitamin B12, but is also a component of at least one mammalian enzyme unassociated with B12. Cobalt is a required trace mineral and has long been administered as a dietary supplement to humans and animals. Cobalt deficiency outside of its requirement in vitamin B12 has not been reported in humans. The administration of cobalt salts was once standard treatment for anaemia in humans, owing to its ability to stimulate red blood cell synthesis. Elemental cobalt acts by stabilising hypoxia inducible …

Large Genomic Differences Between Moraxella Bovoculi Isolates Acquired From The Eyes Of Cattle With Infectious Bovine Keratoconjunctivitis Versus The Deep Nasopharynx Of Asymptomatic Cattle, Aaron M. Dickey, John D. Loy, James L. Bono, Timonthy P. L. Smith, Mike D. Apley, Brian V. Lubbers, Keith D. Dedonder, Sarah F. Capik, Robert L. Larson, Brad J. White, Jochen Blom, Carol G. Chitko-Mckown, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is a recently described bacterium that is associated with infectious bovine keratoconjunctivitis (IBK) or “pinkeye” in cattle. In this study, closed circularized genomes were generated for seven M. bovoculi isolates: three that originated from the eyes of clinical IBK bovine cases and four from the deep nasopharynx of asymptomatic cattle. Isolates that originated from the eyes of IBK cases profoundly differed from those that originated from the nasopharynx of asymptomatic cattle in genome structure, gene content and polymorphism diversity and consequently placed into two distinct phylogenetic groups. These results suggest that there are genetically distinct strains of M. …

Comparison Of Pcr Tests For The Detection Of Mycoplasma Agalactiae In Sheep And Goats, Hüban Göçmen, Ruben Sebastian Rosales, Roger David Ayling, Mi̇hri̇ban Ülgen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare PCR tests for the detection of Mycoplasma agalactiae in sheep and goats. Samples obtained from sheep and goats in Turkey were tested to determine the presence of M. agalactiae. Mycoplasma culture and two direct PCR methods based on the uvrC gene and polC gene were compared with PCR of the 16S rRNA gene followed by denaturing gradient gel electrophoresis (PCR/DGGE) on DNA extracted from clinical samples (following culture enrichment). A total of 234 samples were examined: 9.4% and 13.2% were positive for M. agalactiae based on the uvrC gene and polC gene …

Effects Of Acetic Acid Supplementation In Broiler Chickens Orallychallenged With Salmonella Pullorum, Gulbeena Saleem, Rukhshanda Ramzaan, Farina Malik Khattak, Raheela Akhtar

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the comparative efficacy of three different concentrations of acetic acid on broiler chickens experimentally challenged with Salmonella enterica serovar Pullorum (S. Pullorum). A total of 360 birds were divided into five groups (A?E). Group A served as the unchallenged, untreated control. All the birds in groups B?E were challenged with 4 × 104 cfu/mL of S. Pullorum. Birds in groups C, D, and E were respectively treated with three different concentrations, 0.5%, 1%, and 1.5%, of acetic acid. Pathological examination revealed congested and hemorrhagic liver, hepatitis and necrotic areas in the liver, hydropericardium, focal …

Impact Of Short Compensatory Growth Periods On Performance,Carcass Traits, Fat Deposition, And Meat Properties Of Najdi Lambs, Ahmed Sami, Bander Al-Salbood, Mohamed Abouheif

Turkish Journal of Veterinary & Animal Sciences

Forty-eight Najdi male lambs weighing 33.1 ± 0.06 kg and approximately 4.5 months old were divided into three groups (16 lambs in each) to study the impact of compensatory growth on performance, carcass traits, fat deposition, tissue distribution, and lean chemical composition. The control group was fed ad libitum throughout the entire 12 weeks of the trial period. The other two groups were feed-restricted by 20% and 40% of the ad libitum group intake. Lambs in restricted treatments were feed-restricted for 3 weeks followed by 4 weeks of ad libitum feeding (compensatory) and then 2 weeks of feed restriction followed …

Genetic Analysis Of Avian Paramyxovirus Type I Strains Isolatedfrom Backyard Poultry In Iran, Fereshteh Sabouri, Mehdi Vasfi Marandi, Vahid Karimi, Mohammad Malekan, Mohsen Bashashati

Turkish Journal of Veterinary & Animal Sciences

Newcastle disease (ND) is one of the most serious diseases amongst many species of birds and causes huge economic losses in the poultry sector. Backyard poultry are the main source of food in rural areas of many developing countries and play a crucial role in the epidemiology of ND. The sequence of the F protein cleavage site is the major virulence determinant of Newcastle disease virus (NDV). The aim of this study was to understand the molecular characterization of F proteins of Iranian backyard poultry in NDVs. The complete open reading frame sequences of the F gene of six isolates …

Development Of A Novel In Vivo Corneal Fibrosis Model In The Dog, K. M. Gronkiewicz, Elizabeth A. Giuliano, K. Kuroki, F. Bunyak, Ajay Sharma, L. B. C. Teixeira, C. W. Hamm, R. R. Mohan

Pharmacy Faculty Articles and Research

The aim of this study was to develop a novel in vivo corneal model of fibrosis in dogs utilizing alkali burn and determine the ability of suberanilohydroxamic acid (SAHA) to inhibit corneal fibrosis using this large animal model. To accomplish this, we used seven research Beagle dogs. An axial corneal alkali burn in dogs was created using 1 N NaOH topically. Six dogs were randomly and equally assigned into 2 groups: A) vehicle (DMSO, 2 μL/mL); B) anti-fibrotic treatment (50 μM SAHA). The degree of corneal opacity, ocular health, and anti-fibrotic effects of SAHA were determined utilizing the Fantes grading …

Molecular Mechanisms Of Suberoylanilide Hydroxamic Acid In The Inhibition Of Tgf-Β1-Mediated Canine Corneal Fibrosis, Kristina M. Gronkiewicz, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To investigate molecular mechanisms mediating anti-fibrotic effect of SAHA in the canine cornea using an in vitro model. We hypothesized that SAHA attenuates corneal fibrosis by modulating Smad-dependent and, to a lesser extent, Smad-independent signaling pathways activated by TGF-β1, as well as matrix metalloproteinase (MMP) activity.

Methods—Cultured canine corneal fibroblasts (CCF) were incubated in the presence/absence of TGF-β1 (5ng/ml) and SAHA (2.5μM) for 24hrs. Western blot analysis was used to quantify non-phosphorylated and phosphorylated isoforms of Smad2/3, p38 MAP kinase (MAPK), ERK1/2 and JNK1. Real-time PCR and zymography were utilized to quantify MMP1, MMP2, MMP8 and MMP9 mRNA expression and …

Effect Of Dietary Supplementation With Different Levels Of Inulin-Typefructans On Renal Expression Of Aquaporin 2 Of Growing Piglets, Katarzyna Michalek, Marta Grabowska, Mariusz Skowronski, Adam Lepczynski, Agnieszka Herosimczyk, Maria Laszczynska

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to identify and investigate the expression of aquaporin 2 (AQP2) in the renal tubules of growing piglets fed with different levels of inulin-type fructans. The experiment was performed on five groups of PIC x Penarlan P76 crossbred piglets fed with the following diets: standard diet (control group) and standard diet supplemented with 1% (group I), 2% (group II), and 3% (group III) of water extract of chicory root inulin-type fructans. The animals from the group IV were fed with standard diet supplemented with 4% dried chicory roots. To analyze immunolocalization and expression of AQP2 …

Comparative Efficacy Of Antioxidant Retinol, Melatonin, And Zinc During In Vitro Maturation Of Bovine Oocytes Under Induced Heat Stress, Jafrin Ara Ahmed, Devojyoti Dutta, Nawab Nashiruddullah

Turkish Journal of Veterinary & Animal Sciences

Both A and B grade cumulus-oocyte complexes (COCs) aspirated from cattle ovaries at slaughter were matured in vitro under normal (38.5 °C) and elevated temperatures (41 °C). Maturation competence based on cumulus expansion, COC diameter, and nuclear maturation were compared with and without antioxidant supplementation incorporating 7.5 µM retinol, 1 nM melatonin, and 1.5 µg/mL zinc chloride in an oocyte maturation medium. Heat stress significantly reduced cumulus expansion by approximately 20%, while only retinol could bring a significant (P ≤ 0.05) increase. Heat stress also decreased expansion of A (approximately 50%) and B (approximately 40%) grade COC diameter. All antioxidants …

Cla And Epa Inhibit Lps-Induced Prostaglandin Release From Bovine Endometrial Cells Through An Nf-?B-Dependent Signaling Mechanism, Lokenga Badinga, Mehmet Şükrü Gülay, Alan Ealy

Turkish Journal of Veterinary & Animal Sciences

Although their action in antagonizing arachidonic acid metabolism is a key antiinflammatory effect of conjugated linoleic acid (CLA) and omega-3 polyunsaturated fatty acids (n-3 PUFA), these molecules have other antiinflammatory effects that might occur upstream of altered eicosanoid production. We examined the effects of two CLA isomers (c9,t11 and t10,c12 isomers), n-3 PUFA (EPA and DHA), and ΚNF-ΚB inhibitor (ammonium pyrrolidine dithiocarbamate (PDTC)) on lipopolysaccharide (LPS)-induced secretion of prostaglandins (PGE2 and PGF2a) by bovine endometrial (BEND) cells. LPS increased PGE2 and PGF2a concentrations in BEND cell-conditioned media in a concentration- and time-dependent manner. The c9,t11 CLA isomer and EPA decreased …

Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem

Turkish Journal of Veterinary & Animal Sciences

In this study, the presence of endosymbiotic Wolbachia bacteria was determined by immunohistochemical methods in 8 out of 43 paraffin-embedded bovine aortic tissues that were macroscopically and microscopically diagnosed with aortic onchocerciasis in the archive of the Harran University Faculty of Veterinary Medicine's Department of Pathology. By means of the enzyme histochemical and immunohistochemical staining methods, it was ascertained that the tryptase-containing subtype of mast cells (MCT) was involved in the inflammatory response in the bovine aortae with onchocerciasis. The aim of this study was to elucidate the pathogenesis of aortic onchocerciasis lesions caused by Onchocerca armillata in Turkey and …

Effects Of Full-Fat Soybean Diet On Performance, Carcass Characteristics,And Fatty Acid Composition Of Hanwoo Steers, Sungil Kim, Jihong Lee, Sungkwon Park

Turkish Journal of Veterinary & Animal Sciences

The effect of dietary full-fat soybean on performance and carcass characteristics of Hanwoo steers was investigated. Thirty steers (653 ± 52.96 kg) were allotted to control and full-fat soybean groups with a 210-day comparative feedlot trial. Steers were fed either the basal finishing diet (control) or control plus 5% full-fat soybean, using Calan gates for individual intake measurement. There was no difference in BW, feed intake, carcass weight, backfat thickness, loin muscle area, or meat color between groups. The average daily gain was greater (P < 0.05) in the soybean group compared to the control group. Steers fed soybean showed a lower feed conversion ratio than the controls. Marbling in the soybean group was greater (P < 0.05) than that in the control group. Supplementary full-fat soybean increased (P < 0.05) crude fat but decreased (P < 0.05) crude protein content in the longissimus muscle. Full-fat soybean increased (P < 0.05) total unsaturated fatty acids, including the levels of oleic, linoleic, and linolenic acid but, consequently, decreased (P < 0.05) total saturated fatty acids. Levels of conjugated linoleic acid, and its major precursor trans-vaccenic acid, were significantly increased by soybean supplementation. Our results demonstrate that full-fat soybeans may offer a better way to produce healthier beef with increased unsaturated fatty acids.

Effect Of Stocking Rate On Pasture And Sheep Production In Winter And Spring Lambing Systems, Serkan Ateş, Gazi̇ Özcan, Harun Çi̇çek, Mesut Kirbaş, Gürhan Keleş, Şaban Işik

Turkish Journal of Veterinary & Animal Sciences

A 2-year study was conducted to quantify the effect of stocking rate on pasture and sheep production in winter and spring lambing systems. Pastures were set-stocked with ewes and their winter-born single lambs at low (20 ewes + lamb ha?1), medium (28 ewes + lamb ha?1), and high (36 ewes + lamb ha?1) stocking rates in spring 2012 and by ewes and their spring-born lambs at low (16 ewes + lamb ha?1), medium (24 ewes + lamb ha?1), and high (32 ewes + lamb ha?1) stocking rates in spring 2013. Annual pasture dry matter production ranged from 8.9 to 10.2 …

Productivity And Adaptation Ability Of Holstein Cattle Of Different Genetic Selections, Ivan Fiodorovich Gorlov, Svetlana Evgenievna Bozhkova, Olga Pavlovna Shakhbazova, Vera Vasilievna Gubareva, Natali Ivanovna Mosolova, Elena Yurievna Zlobina, Yuriy Nicolaevich Fiodorov, Alexey Sergeevich Mokhov

Turkish Journal of Veterinary & Animal Sciences

The purpose of the research was to compare economically useful traits of Holstein cows of different selections. The results show that the German and Australian selections were superior to the Danish and American cows in terms of their growth (at 36 months of age, the body weights of the German and Australian cows were 36.5 and 27.6 kg greater than the Danish cows and 24.3 and 15.4 kg greater than the American cows, respectively). Maximum milk yield was obtained from the American and German cows (25,220 and 24,861 kg). Their milk also had higher fat content. The maximum protein content …

Poor Placental Traits Reduce Kid Birth Weight In Young Saanen Dams In The First Parity, Uğur Şen, Hasan Önder

Turkish Journal of Veterinary & Animal Sciences

Placental characteristics are the most important indicators of fetal growth and development that influence kid vitality. The aim of this study was to examine the effect of maternal parity on placental characteristics and birth-related traits in Saanen goats. The experiments were conducted on 41 singleton-bearing Saanen does, ranging from 1 to 4 years of age and classified according to first, second, and third parities. Birth weight, placental weight, placental efficiency, and cotyledon density were influenced by parity (P < 0.05). First-parity does had kids with significantly (P < 0.05) lower birth and placental weights than does of other parities, while they had significantly higher (P < 0.05) placental efficiency and cotyledon density. First-parity does had fewer (P < 0.05) large and total cotyledons than third-parity does. Cotyledon dimensions varied among parities (P < 0.05), while average cotyledon surface area and cotyledon efficiency were the lowest in the first-parity does (P < 0.05). The results suggest that younger dams in the first parity may alter placental characteristics and fetal development, resulting in a reduced birth weight from singleton gestations.

Mycoplasma Infections In Dairy Cattle Farms In Turkey, Zafer Sayin, Asli Sakmanoğlu, Uçkun Sai̇t Uçan, Ali̇ Uslu, Hasan Hüseyi̇n Hadi̇mli̇, Zeki̇ Aras, Özgür Özdemi̇r, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

Mycoplasmas cause the most severe and economically costly diseases of cattle throughout the world. In this study, Mycoplasma species were isolated from calves and cows with suspected mycoplasmosis in Holstein dairy cattle farms within 7 geographical regions of Turkey between May 2010 and December 2015. Mycoplasma infections were positive in 17 (80.9%) of 21 dairy cattle farms and the overall percentage was calculated as 32.1%. The highest isolation rate occurred in the Southeastern Anatolia Region (42.8%), and the lowest was observed in the Mediterranean Region (19.6%). In total, 172 Mycoplasma spp. were isolated from samples. Using PCR analysis, 149 (87.6%) …

Comparison Of Foetal Growth In Singleton And Twin Pregnancies Byb-Mode And Doppler Ultrasonography In Karya Ewes, Güneş Erdoğan, Hayretti̇n Çeti̇n, Ahmet Ceylan, İlker Seri̇n, Hakki Bülent Beceri̇kli̇soy

Turkish Journal of Veterinary & Animal Sciences

Foetal growth and haemodynamic features were investigated in singleton and twin pregnancies in Karya ewes with B-mode/Doppler ultrasonography. Nineteen healthy singleton (n = 9) and twin (n = 10) pregnant Karya ewes were used. Foetal biometric and haemodynamic measurements were made at 15-day intervals on the 29 foetuses during pregnancy. Biparietal head diameter (BPD), thoracic diameter (TD), umbilical cord diameter (UCD), foetal heart rate (FHR), peak systolic velocity (PSV), end diastolic velocity (EDV), pulsatility index (PI), and resistance index (RI) were recorded. The BPD was higher in twins on days 55 and 130 of pregnancy (P < 0.05), and the TD was higher in singletons on day 115 of pregnancy (P < 0.01). UCD was higher in twins on day 55 of pregnancy (P < 0.01). On day 130 of pregnancy, PSV and EDV were higher in twin foetuses (P < 0.05). PI and RI were lower in twins on day 40 of pregnancy (P < 0.05). Foetal growth and umbilical artery blood flow show some differences depending on the numbers of foetuses and the gestational stage. Additionally, BPD, TD, and FHR were reliable for estimation of gestational age during twin pregnancies.

Effects Of Combining Recombinant Bovine Growth Hormone And Anabolic Implants On Growth Performance And Dietary Energetics Of Holstein Bull Calves Fed Finishing Diets, Victor Gonzalez-Vizcarra, Maria Lopez-Soto, Carolina Pujol-Manriquez, Jesus Urias-Estrada, Alberto Barreras Serrano, Jose Calderon-Cortes, Gilberto Lopez-Valencia, Francisco Monge-Navarro, Alejandro Plascencia

Turkish Journal of Veterinary & Animal Sciences

Twenty-one Holstein bull calves (368 ± 5.2 kg) were used in order to evaluate the effects of combining recombinant bovine growth hormone (bST) and anabolic implants on growth performance and dietary energetics. Steers were assigned (7 repetitions/treatment) to individual pens (16 m2) equipped with automatic waterers and 1.2 m fence-line feed bunks. The experiment lasted 56 days. Treatments were: 1) no bST, no implant; 2) implant; and 3) bST + implant. Compared to implanted cattle, the combination of exogenous bovine somatotropin and anabolic implants did not enhance growth performance or observed dietary energy. Compared to nonimplanted cattle, anabolic implants increased …

Early Pregnancy Diagnosis Using A Commercial Elisa Test Based On Pregnancy-Associated Glycoproteins In Holstein-Friesian Heifers And Lactating Cows, Mehmet Sali̇h Kaya, Mehmet Köse, Faruk Bozkaya, Hasan Mutlu, Eyyüp Hakan Uçar, Mehmet Osman Atli

Turkish Journal of Veterinary & Animal Sciences

In this study, we aimed to investigate the efficacy of a commercial ELISA test kit for detecting pregnancy-associated glycoproteins (PAGs) in peripheral blood for early pregnancy diagnosis and compare plasma PAG levels during early pregnancy in both Holstein-Friesian heifers and lactating cows. A total of 231 plasma samples were collected from heifers and lactating cows on days 25, 28, and 32 after insemination. Pregnancies were confirmed 30 days after the collection of plasma samples. Plasma PAG levels were measured using a commercial ELISA test kit for diagnosing bovine pregnancy. The effects of examination date and animal status (heifers vs. lactating …

Circulating Metabolic And Reproductive Hormone Changes In Laying Hens Kept Under Various Heat-Combating Systems, Muhammad Safdar Anjum, Mansur Abdullah Sandhu, Zia Ur-Rahman, Anum Safdar

Turkish Journal of Veterinary & Animal Sciences

The objective of the present study was to observe the circulating metabolic, reproductive, and stress hormones in laying hen performance under different heat-combating systems (HCSs). A total of 500 White Leghorn pullets at 24 weeks of age were subjected to four HCSs, i.e. desert cooling (DC), water sprinkling (WS), time limit feeding (TLF), and ascorbic acid (AA) supplementation, at environmental temperatures from 32.30 to 40.80 °C with 76.40% relative humidity. Blood was collected before heat stress, at peak stress, and after heat stress to collect plasma. The results of the 24-week stress period showed significantly less circulating T3, T4, estrogen, …