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Veterinary Medicine Commons

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2016

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Articles 121 - 150 of 320

Full-Text Articles in Veterinary Medicine

Circulating Amino Acids In Blood Plasma During The Peripartal Period In Dairy Cows With Different Liver Functionality Index, Z. Zhou, J. J. Loor, F. Piccioli-Cappelli, F. Librandi, G. E. Lobley, E. Trevisi Mar 2016

Circulating Amino Acids In Blood Plasma During The Peripartal Period In Dairy Cows With Different Liver Functionality Index, Z. Zhou, J. J. Loor, F. Piccioli-Cappelli, F. Librandi, G. E. Lobley, E. Trevisi

Physiology Collection

The liver functionality index (LFI) measures the changes of albumin, cholesterol, and bilirubin concentrations between 3 and 28 d postpartum. This composite index, based on variables with direct relevance to liver-specific plasma protein synthesis (albumin), hepatic/intestinal lipoprotein synthesis (cholesterol), and clearance of breakdown products of heme catabolism (bilirubin), provides a tool for evaluating manifestations of hepatic disease. Both energy and protein metabolism are likely to be affected by various physiological challenges in this period but have not been tested systematically. The present study was conducted to profile AA in cows with high or low LFI during the peripartal period …


The Effect Of Various Erythrocyte Species On The Detection Of Avian, Swine And Canine Influenza A Viruses Isolated In Thailand, Aunyaratana Thontiravong, Duangduean Prakairungnamthip, Sunicha Chanvatik, Nutthawan Nonthabenjawan, Wikanda Tunterak, Ratanaporn Tangwangvivat, Kanisak Oraveerakul, Alongkorn Amonsin Mar 2016

The Effect Of Various Erythrocyte Species On The Detection Of Avian, Swine And Canine Influenza A Viruses Isolated In Thailand, Aunyaratana Thontiravong, Duangduean Prakairungnamthip, Sunicha Chanvatik, Nutthawan Nonthabenjawan, Wikanda Tunterak, Ratanaporn Tangwangvivat, Kanisak Oraveerakul, Alongkorn Amonsin

The Thai Journal of Veterinary Medicine

Hemagglutination (HA) and hemagglutination inhibition (HI) tests are conventional, well-known methods used for the detection of influenza A viruses (IAVs) and antibodies to IAVs, respectively. The sensitivity of HA and HI tests is usually affected by the compatibility of sialic acid (SA) receptors on red blood cells (RBCs) with the HA proteins of IAVs. In this study, the erythrocyte binding preferences of sixteen avian, swine and canine IAVs from Thailand were investigated using five erythrocyte sources: chicken, turkey, goose, guinea pig and horse RBCs. The results demonstrated that, turkey RBCs yielded the highest HA titers against most avian, swine and …


Control Of Estrus In Gilts And Primiparous Sows, Roy N. Kirkwood, Fabio De Rensis Mar 2016

Control Of Estrus In Gilts And Primiparous Sows, Roy N. Kirkwood, Fabio De Rensis

The Thai Journal of Veterinary Medicine

The primary controller of weaned pig output is the successful breeding of enough females in the breeding week. The number of females available for breeding is dependent on the number of sows weaned sows and service-ready gilt availability. Primiparous (P1) sows often have longer wean-estrus intervals (WEI) and increased anestrus; ensuring sufficient gilts and sows are available may require control of estrus. Stimulating estrus requires appropriate boar contact or when this is not sufficient, administration of gonadotrophins. If gonadotrophins are used expect estrus 4 to 6 d later and it is usual to breed at the induced estrus. If there …


Comparison Of Effects Of Different Antioxidants Supplemented To Long-Term Extender On Boar Semen Quality Following Storage At 17ºc, Thevin Vongpralub, Pachara Thananurak, Chamaiporn Sittikasamkit, Phirawit Chuawongboon, Monchai Duangjinda, Wuttigrai Boonkum, Vibuntita Chankitisakul Mar 2016

Comparison Of Effects Of Different Antioxidants Supplemented To Long-Term Extender On Boar Semen Quality Following Storage At 17ºc, Thevin Vongpralub, Pachara Thananurak, Chamaiporn Sittikasamkit, Phirawit Chuawongboon, Monchai Duangjinda, Wuttigrai Boonkum, Vibuntita Chankitisakul

The Thai Journal of Veterinary Medicine

The use of antioxidants as semen additives in the extender has been proposed to improve sperm function during cool storage. The objective of this study was to compare the effects of different antioxidant supplementation in extender on boar semen quality during storage for 10 days at 17ºC. Semen samples (n=18) were divided into 10 aliquots and diluted with Reading extender supplemented with three different concentrations of water-soluble glutathione, L-carnitine, and vitamin E analogue Trolox (0.1, 1 and 5mM). All samples were stored in a refrigerator at 17ºC for 10 days. Sperm motility was assessed at Days 1, 4, 7 and …


Evaluation Of Amelogenin And Zinc-Finger Loci For Sex Identification In Captive Felids, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr Mar 2016

Evaluation Of Amelogenin And Zinc-Finger Loci For Sex Identification In Captive Felids, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

The amelogenin and zinc-finger regions were used for sex determination in six species of felids: Felis catus (Persian cat), Felis chaus (jungle cat), Prionailurus bengalensis (leopard cat), Pardofelis temminckii (Asiatic golden cat), Panthera tigris (tiger) and Prionailurus viverrinus (fishing cat). The amelogenin region in all six felids could be successfully amplified and gave a heterozygous genotype in males and a homozygous genotype in females, except for leopard cats which showed a heterozygous genotype in both sexes. The PCR products from the zinc-finger region could be amplified in Persian cats, Asiatic golden cats, tigers and fishing cats. In the Persian cat, …


Cisplatin Intralesional Chemotherapy Of Recurrence Schwannoma In Horse: A Case Report, Rungrueang Yodsheewan, Kanittha Phetudomsinsuk Mar 2016

Cisplatin Intralesional Chemotherapy Of Recurrence Schwannoma In Horse: A Case Report, Rungrueang Yodsheewan, Kanittha Phetudomsinsuk

The Thai Journal of Veterinary Medicine

A 7-year-old crossbred stallion had multiple solitary cutaneous masses at the right infraorbital area which were recurrent for two months. The biggest mass size was 5 x 7 x 1 cm3 and had superficial bleeding. This case was diagnosed as peripheral nerve sheath tumors based on cytological and histological examination which showed numerous spindle cells with cancerous characteristics, cell patterns of the tumor were interlacing bundle and a whirl. Most areas showed tense palisading nuclei of tumor cells (Verocay bodies) known as Antoni A area. The rest showed loose paucicellular tissue known as Antoni B area. Within histologic sections, the …


Effect Of Antibiotic Growth Promoters On Anti-Oxidative And Anti-Inflammatory Activities In Broiler Chickens, Krittika Kabploy, Nuntavan Bunyapraphatsara, Noppawan Phumala Morales, Nuanchan Paraksa Mar 2016

Effect Of Antibiotic Growth Promoters On Anti-Oxidative And Anti-Inflammatory Activities In Broiler Chickens, Krittika Kabploy, Nuntavan Bunyapraphatsara, Noppawan Phumala Morales, Nuanchan Paraksa

The Thai Journal of Veterinary Medicine

The current study was conducted to evaluate the effects of sub-therapeutic antibiotics (avilamycin and flavophospholipol) in animal feed on anti-oxidative and anti-inflammatory properties in broilers to understand the mechanisms of AGPs. Four hundred and fifty six 1-day-old broilers (ROSS 308) were divided into 3 groups with 4 replications. Three experimental diets composed of a corn-soybean meal based diet supplemented with 0, 10 mg/kg avilamycin and 5 mg/kg flavophospholipol were randomly provided to the broilers for 42 days, respectively. Results showed that the AGPs significantly decreased TBA-MDA adduct in serum as well as villi tissue of the broilers, especially flavophospholipol. However, …


Study Of Genetic Variation Of Captive Asiatic Golden Cat (Pardofelis Temminckii) In Thailand Using Domestic Cat (Felis Catus) Microsatellite Markers, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr Mar 2016

Study Of Genetic Variation Of Captive Asiatic Golden Cat (Pardofelis Temminckii) In Thailand Using Domestic Cat (Felis Catus) Microsatellite Markers, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

Domestic cat (Felis catus) microsatellite markers were used to determine genetic diversity in 17 captive Asiatic golden cats (Pardofelis temminckii, syn. Catopuma temminckii). Allele numbers, genotype numbers, genetic diversity, heterozygosity, polymorphism information content (PIC) and inbreeding coefficient (f) were observed in each marker. The average values of genotype number, allele number, expected heterozygosity, observed heterozygosity, polymorphism information content (PIC) and inbreeding coefficient (f) were 6.12, 4.71, 0.70, 0.29, 0.65 and 0.62, respectively. These results revealed a reduction in the genetic diversity as shown by the lower value for the observed heterozygosity compared to the expected heterozygosity and the high value …


Aflatoxin Effect On Humoral And Mucosal Immune Responses Against Infectious Bronchitis Vaccine In Broilers, Babak Shahabi-Ghahfarokhi, Majid Gholami-Ahangaran, Mohsen Jafarian-Dehkordi Mar 2016

Aflatoxin Effect On Humoral And Mucosal Immune Responses Against Infectious Bronchitis Vaccine In Broilers, Babak Shahabi-Ghahfarokhi, Majid Gholami-Ahangaran, Mohsen Jafarian-Dehkordi

The Thai Journal of Veterinary Medicine

The aflatoxin effect on humoral and mucosal immunity against infectious bronchitis (IB) vaccine was studied in 225 one-day-old chicks. The chicks were divided in 3 equal groups. Treatment groups included group A: chickens fed a basal diet, and group B: chickens fed 3 ppm productive aflatoxin in a basal diet. The chickens in groups A and B were vaccinated against IB (at one and 10 days old) by H120 live attenuated vaccine. For group C, chickens did not receive aflatoxin and were not vaccinated against IB. All chickens received experimental diets from 3 to 28 days old, continuously. At 28 …


Foal Deciduous Teeth Stem Cells Enhance Wound Healing In Rabbit Wound Model, Nitipon Srionrod, Ratikorn Bootcha, Soontaree Petchdee Mar 2016

Foal Deciduous Teeth Stem Cells Enhance Wound Healing In Rabbit Wound Model, Nitipon Srionrod, Ratikorn Bootcha, Soontaree Petchdee

The Thai Journal of Veterinary Medicine

The purpose of this study was to evaluate the potential use of deciduous teeth stem cells from horses for wound healing in animal wound model. This study assessed the efficacy and safety of foal deciduous teeth stem cells (fDSCs) administration in rabbit excisional wound model. Deciduous teeth stem cells were harvested from horses and full thickness excisional wound was made on rabbits by surgical removal of epidermis and dermis to the depth of subcutaneous fat. New Zealand White rabbits (n=8) were divided into a control group (n=4) and a treatment group (n=4). Multiple injections of fDSCs suspension were intravenously administered …


Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Sirilak Disatian Surachetpong, Pakit Boonpala Mar 2016

Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Sirilak Disatian Surachetpong, Pakit Boonpala

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich Mar 2016

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Application Of Real-Time Pcr For Quantifying Gastrointestinal Microbiota In Weaning Pigs Influenced By Dietary Feed Additive Supplementation, Phattarapon Chaloemnon, Mullika Traidej Chomnawang, Wirawan Junlapho, Nuanchan Paraksa Mar 2016

Application Of Real-Time Pcr For Quantifying Gastrointestinal Microbiota In Weaning Pigs Influenced By Dietary Feed Additive Supplementation, Phattarapon Chaloemnon, Mullika Traidej Chomnawang, Wirawan Junlapho, Nuanchan Paraksa

The Thai Journal of Veterinary Medicine

To apply real-time polymerase chain reaction (PCR) to detect and quantify the gastrointestinal microbiota modulated by dietary feed additives was the objective of this study. Sixty-four three-crossbred weaning piglets at 24 days were used and randomly assigned to be fed one of the experimental diets supplemented with different feed additives (0.2% inulin, 0.1% fructooligosaccharide and sub-therapeutic antibiotics), and compared with the non-supplemented group. Comparison of microbiota enumeration by conventional culture and real-time PCR methods in response to feed additives was investigated. The microbiota enumeration by conventional culture and real-time PCR methods showed corresponding responses of selected bacterial counts (Lactobacilli, Bifidobacteria, …


Use Of Different Models For Estimation Of Genetic Parameters And Genetic Trends Of Performance Test Traits Of Gilts, Dragomir Lukač Mar 2016

Use Of Different Models For Estimation Of Genetic Parameters And Genetic Trends Of Performance Test Traits Of Gilts, Dragomir Lukač

The Thai Journal of Veterinary Medicine

The aims of this research were to compare estimates of variance components using different animal models and to determine the most suitable mixed model for estimating genetic parameters and genetic trends for traits in performance test of gilts of different breeds using REML. A total of 73129 gilts of four genotypes in the period of 2009 to 2013 were included in the analyses. Four mixed models were constructed. Information criterion of Akaike (AIC) and Bayesian information criterion (BIC) were used to suggest which model is an adequate model for evaluation of genetics parameters. With the introduction of certain factors in …


Prevalence Of Subclinical Laminitis And Its Effects On Reproductive Performance In Lactating Cows In Thailand, Suvaluk Seesupa, Kwankate Kanistanon, Rittichai Pilachai, Suneerat Aiumlamai Mar 2016

Prevalence Of Subclinical Laminitis And Its Effects On Reproductive Performance In Lactating Cows In Thailand, Suvaluk Seesupa, Kwankate Kanistanon, Rittichai Pilachai, Suneerat Aiumlamai

The Thai Journal of Veterinary Medicine

The objectives of this study were to investigate the prevalence of subclinical laminitis and to evaluate its effects on reproductive performances in dairy cows in Thailand. Ninety-eight lactating cows from 22 smallholder dairy farms and 138 lactating cows from one large scale dairy farm were assessed for subclinical laminitis by evaluating lesions after hoof trimming. Hemorrhagic lesions of the sole and white line area were evaluated. Any appearance of sole hemorrhage or white line hemorrhage scoring 2 on one or more claws was defined as a case of subclinical laminitis. Reproductive data were recorded and analyzed using subclinical laminitis as …


Activated Partial Thromboplastin Time (Aptt) In Dogs Tested By In-House Crude Cephalin From Canine Brain, Sarawut Yama, Am Chaimongkhol, Somsak Channoi, Sakhorn Pornprasert, Wanna Suriyasathaporn Mar 2016

Activated Partial Thromboplastin Time (Aptt) In Dogs Tested By In-House Crude Cephalin From Canine Brain, Sarawut Yama, Am Chaimongkhol, Somsak Channoi, Sakhorn Pornprasert, Wanna Suriyasathaporn

The Thai Journal of Veterinary Medicine

This research aimed to identify the reference value of activated partial thromboplastin time (APTT) in normal dogs using crude cephalin prepared in-house from canine brain, and to examine its efficacy in detecting disorders of coagulation factors. A total of 105 blood samples were collected from normal dogs between January-May 2010. The APTT value was found to be 10.43-19.08 seconds and significantly correlated with the APTT value of a test using a commercial reagent (Pearson’s correlation, r = 0.9923, p < 0.001). On testing the reagent’s efficacy in detecting disorders of coagulation factors by using cryopoor plasma, it was discovered that the APTT value ranged from 24.50 to 33.44 seconds, and the mean value of the APTT by means of cryopoor plasma was significantly greater than that of normal dogs (p < 0.001). The aforementioned APTT value significantly correlated with that obtained by means of a commercial reagent (Pearson’s correlation, r = 0.9984, p < 0.001). From this research, it can be confirmed that in-house crude cephalin can be substituted for commercial crude cephalin. Besides, the obtained reference range can further be applied in veterinary hematology laboratory.


Comparison Of Two Methods For The Recovery Of Campylobacter From Chicken Carcasses, Suthida Muangnoicharoen, Sakaoporn Prachantasena, Luck Hankla, Taradon Luangtongkum Mar 2016

Comparison Of Two Methods For The Recovery Of Campylobacter From Chicken Carcasses, Suthida Muangnoicharoen, Sakaoporn Prachantasena, Luck Hankla, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

Campylobacteriosis, one of the most common food-borne diseases in humans, is mainly caused by the consumption of chicken meat contaminated with Campylobacter. In this study, we examined the recovery rate of Campylobacter from carcass rinse using direct plating method and selective enrichment method. Campylobacter genotypes obtained from both methods were also determined using flaA-short variable region (flaA-SVR) sequencing. Results showed that Campylobacter recovery rates by the direct plating method and selective enrichment method were 40.00% and 46.86%, respectively. However, when a combination of the two methods was used, the recovery rate increased to 64.57%. For genetic characterization of Campylobacter, 9 …


Evaluation Of Pain And Prostate Volume Of Dogs Treated With Intra-Prostatic Injection Of Botulinum Toxin Type A, Pintira Thiangthientham, Kaywalee Chatdarong, Suppawiwat Ponglowhapan Mar 2016

Evaluation Of Pain And Prostate Volume Of Dogs Treated With Intra-Prostatic Injection Of Botulinum Toxin Type A, Pintira Thiangthientham, Kaywalee Chatdarong, Suppawiwat Ponglowhapan

The Thai Journal of Veterinary Medicine

Recently, alternative medical treatment of benign prostatic hyperplasia (BPH) using intra-prostatic injection of botulinum toxin type A (BT-A) has been reported in men and mice. However, in terms of animal welfare, it remains questionable when applied to clinical use. This study aimed at evaluating the effects of intra-prostatic injection of BT-A (Botox, Allergan, USA) in dogs. Six healthy intact males with no clinical signs of BPH received 100 units of BT-A by ultrasound-guided transabdominal intra-prostatic injection (50 units of each prostatic lobe). Pain scoring, evaluation of white blood cell counts, cortisol concentrations, and observation of urination behaviour were recorded before …


What Is Your Diagnosis?, Nan Choisunirachon Mar 2016

What Is Your Diagnosis?, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.


Seroprevalence Of Influenza A In Domestic Dogs In Thailand, 2013, Sunicha Chanvatik, Ratanaporn Tangwangvivat, Supassama Chaiyawong, Duangduean Prakairungnamthip, Ranida Tuanudom, Aunyaratana Thontiravong, Alongkorn Amonsin Mar 2016

Seroprevalence Of Influenza A In Domestic Dogs In Thailand, 2013, Sunicha Chanvatik, Ratanaporn Tangwangvivat, Supassama Chaiyawong, Duangduean Prakairungnamthip, Ranida Tuanudom, Aunyaratana Thontiravong, Alongkorn Amonsin

The Thai Journal of Veterinary Medicine

Canine influenza viruses (CIVs) have been reported in dogs worldwide. Due to the relatively high risk of influenza A virus (IAV) infections in dogs and humans through dog-human interface, the seroprevalence of IAVs in dogs in Thailand was surveyed. A serological survey of IAV infections in dogs in Thailand was conducted from December 2012 to November 2013 using ELISA assays for detecting anti-nucleoprotein (NP) antibodies (NP-ELISA) and hemagglutination inhibition (HI) tests for detecting HA-specific antibodies using canine H3N2 (CIV-H3N2), human pandemic H1N1 2009 (Human-pH1N1) and human seasonal H3N2 (Human-H3N2) as viral antigens. A total of 9,891 serum samples were obtained …


Association Between Cephalexin Administration And Emergence Of Methicillin-Resistant Coagulase-Positive Staphylococci (Mrcops) In Dogs, Punpichaya Fungwithaya, Pattrarat Chanchaithong, Nathita Phumthanakorn, Passana Muaungkong, Pitikarn Bumpenpol, Mutchamon Kaewparuehaschai, Chanwit Tribudharat, Nuvee Prapasarakul Mar 2016

Association Between Cephalexin Administration And Emergence Of Methicillin-Resistant Coagulase-Positive Staphylococci (Mrcops) In Dogs, Punpichaya Fungwithaya, Pattrarat Chanchaithong, Nathita Phumthanakorn, Passana Muaungkong, Pitikarn Bumpenpol, Mutchamon Kaewparuehaschai, Chanwit Tribudharat, Nuvee Prapasarakul

The Thai Journal of Veterinary Medicine

The potential transmission of methicillin-resistant coagulase-positive staphylococci (MRCoPS) between dogs and human has been noted as of potential public health concern. The current study aimed to determine the emergence of methicillin-resistant coagulase-positive staphylococci (MRCoPS) in dogs after oral administration of cephalexin. Skin swabs from 38 dogs without a history of antibiotic exposure were collected before drug administration (pre-treatment dogs) and during drug administration within one month (treatment dogs). A total of 196 CoPS were isolated from the nose, perineum and skin lesions. Fewer MRCoPS were isolated from the pre-treatment dogs (7.89%) than from the treatment dogs (p<0.001). Methicillin-resistant Staphylococcus (S.) schleiferi subsp. coagulans (MRSSc) were only recovered from the treatment dogs, whereas methicillin-resistant S. pseudintermedius (MRSP) were found in both groups. Overall, a high incidence of MRSP was found since the first week after administration. The nose and perineum were confirmed as the most common site of carriage of MRCoPS rather than the skin lesions. In conclusion, the oral cephalexin administration was associated with the emergence of MRCoPS on dog skin, a potential source of contamination to humans.


Genetic Detection And Identification Of Chlamydophila Psittaci In Captive Psittacine Birds In Thailand, Parut Suksai, Benchapol Lorsunyaluck, Patcharida Dittawong, Pornchai Sanyathitiseree, Preeda Lertwatcharasarakul Mar 2016

Genetic Detection And Identification Of Chlamydophila Psittaci In Captive Psittacine Birds In Thailand, Parut Suksai, Benchapol Lorsunyaluck, Patcharida Dittawong, Pornchai Sanyathitiseree, Preeda Lertwatcharasarakul

The Thai Journal of Veterinary Medicine

Chlamydophilosis is an important, worldwide zoonosis caused by Chlamydophila psittaci. The aim of this study was to detect C. psittaci on choanal and cloacal swabs which were collected from 178 captive psittacine birds (23 different species) from eight provinces in Thailand. ompA gene detection using nested PCR showed that 7.87% (n=14) were positive for C. psittaci. The C. psittaci detection from two site collections (choanal and cloacal swabs) using nested PCR was more sensitive than from one site collection. Most positive samples (n=11) were from asymptomatic adult birds and three other positive birds showing clinical signs were juvenile birds. As …


Influence Of Oil Or Fat Supplementation On Rumen Fermentation Characteristics And Ruminal Fluid Fatty Acid Profile In Brahman Crossbred Fattening Steers, Pitunart Noosen, Pipat Lounglawan, Wisitiporn Suksombat Mar 2016

Influence Of Oil Or Fat Supplementation On Rumen Fermentation Characteristics And Ruminal Fluid Fatty Acid Profile In Brahman Crossbred Fattening Steers, Pitunart Noosen, Pipat Lounglawan, Wisitiporn Suksombat

The Thai Journal of Veterinary Medicine

The aim of this experiment was to study the influence of oil or fat supplementation on rumen fermentation and ruminal fluid fatty acid profiles in Brahman crossbred steers. Twelve steers (87.5% Brahman crossbred) with average live weight (LW) of 337+54 kg and approximate age of 2 years old were stratified by their LW into 4 groups and each group was randomly assigned to four dietary treatments. All steers were fed 14% CP concentrate. The treatments included: 1) 7 kg/d concentrate, 2) 4 kg/d concentrate supplemented with 200 g/d palm oil (PO), 3) 4 kg/d concentrate supplemented with 100 g/d PO …


Distribution Of Tem-1 Gene In Salmonella Enterica Isolated From Poultry Carcasses In Iran, Abbas Doosti, Ali Zohoor, Mohammad Chehelgerdi, Abbas Mokhtari-Farsani Mar 2016

Distribution Of Tem-1 Gene In Salmonella Enterica Isolated From Poultry Carcasses In Iran, Abbas Doosti, Ali Zohoor, Mohammad Chehelgerdi, Abbas Mokhtari-Farsani

The Thai Journal of Veterinary Medicine

Salmonella is one of the most important bacteria isolated from poultry and a major source of bacterial infection to human. The aim of present research was to study TEM-1 beta-lactamase gene in serovars of S. enterica subsp. enterica isolated from poultry carcasses samples in Iran. A total of 600 samples of poultry carcasses were collected and Salmonella was isolated from the samples using bacterial culture methods and genomic DNA was extracted using DNA extraction kit. For the final approval of Salmonella, identification of S. enterica subsp. enterica, S. enterica serovars and TEM-1 gene sequence-specific targets (16S rRNA, fliC, rfbJ, fljB …


Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta Mar 2016

Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

Involucrin (INV) is a cornified envelope precursor protein that is commonly used as an early stage marker of human terminal keratinocyte differentiation. This research investigated the expression of INV in canine skin tumors using 56 formalin-fixed paraffin-embedding (FFPE) biopsied specimens. The expression of INV was investigated by immunohistochemistry (IHC) and RT-PCR. Correlation between INV and Ki-67 was also performed. The malignant tumor groups showed significantly different INV staining scores and Ki-67 positive cells than the normal skin group by IHC (p<0.05). The INV scores of moderately differentiated SCC (MSCC), trichoblastoma (TCB), basal cell carcinoma (BCC) and poorly differentiated SCC (PSCC) significantly decreased, with a statistically significant difference (p<0.05). Interestingly, the INV of poorly differentiated SCC disappeared in some specimens. The INV expression in most tumors was found to be inversely proportionate to Ki-67 positive cells. In addition, only SCC regardless of tumor grade without tumor-graded specimens showed an inversely statistically significant difference between the Ki-67 positive cells and INV expression (p<0.0001). In RT-PCR, mRNA expression of the FFPE specimens showed substantial similarity of INV expression as in IHC. It is suggested that the INV expression should be further studied in terms of clinical prognosis in canine cutaneous SCC.


Mechanical Conflict System: A Novel Operant Method For The Assessment Of Nociceptive Behavior, Steven E. Harte, Jessica B. Meyers, Renee R. Donahue, Bradley K. Taylor, Thomas J. Morrow Feb 2016

Mechanical Conflict System: A Novel Operant Method For The Assessment Of Nociceptive Behavior, Steven E. Harte, Jessica B. Meyers, Renee R. Donahue, Bradley K. Taylor, Thomas J. Morrow

Physiology Faculty Publications

A new operant test for preclinical pain research, termed the Mechanical Conflict System (MCS), is presented. Rats were given a choice either to remain in a brightly lit compartment or to escape to a dark compartment by crossing an array of height-adjustable nociceptive probes. Latency to escape the light compartment was evaluated with varying probe heights (0, .5, 1, 2, 3, and 4 mm above compartment floor) in rats with neuropathic pain induced by constriction nerve injury (CCI) and in naive control rats. Escape responses in CCI rats were assessed following intraperitoneal administration of pregabalin (10 and 30 mg/kg), morphine …


Taylor, Charles Forman, Jr., 1862-1946 (Sc 2975), Manuscripts & Folklife Archives Feb 2016

Taylor, Charles Forman, Jr., 1862-1946 (Sc 2975), Manuscripts & Folklife Archives

Manuscript Collection Finding Aids

Finding aid and scan (Click on "Additional Files" below) for Manuscripts Small Collection 2975. Letter, 21 October 1939, written by druggist Charles Forman Taylor Jr., Bowling Green, Kentucky to Joe Werthan, Nashville, Tennessee, describing a cough syrup to be used on Mr. Werthan’s horses. The letter includes a list of Taylor’s other equine medications with prices.


Evaluation Of Calving Indicators Measured By Automated Monitoring Devices To Predict The Onset Of Calving In Holstein Dairy Cows, V. Ouellet, E. Vasseur, W. Heuwieser, O. Burfeind, X. Maldague, É. Charbonneau Feb 2016

Evaluation Of Calving Indicators Measured By Automated Monitoring Devices To Predict The Onset Of Calving In Holstein Dairy Cows, V. Ouellet, E. Vasseur, W. Heuwieser, O. Burfeind, X. Maldague, É. Charbonneau

Physiology Collection

Dystocias are common in dairy cows and often adversely affect production, reproduction, animal welfare, labor, and economics within the dairy industry. An automated device that accurately predicts the onset of calving could potentially minimize the effect of dystocias by enabling producers to intervene early. Although many well-documented indicators can detect the imminence of calving, research is limited on their effectiveness to predict calving when measured by automated devices. The objective of this experiment was to determine if a decrease in vaginal temperature (VT), rumination (RT), and lying time (LT), or an increase in lying bouts (LB), as measured by …


The Effects Of Acidified Pepsin On Porcine Vocal Fold Tissue: Developing A Porcine Model Of Laryngopharyngeal Reflux Disease, Abigail Cox Durkes Feb 2016

The Effects Of Acidified Pepsin On Porcine Vocal Fold Tissue: Developing A Porcine Model Of Laryngopharyngeal Reflux Disease, Abigail Cox Durkes

Open Access Dissertations

Approximately 7.5 million Americans are affected by a voice disorder. In the last year, 7.2% of people missed one or more days of work due to a voice problem. For professional voice users, such as teachers, the rate increases to 20% and an annual cost of $2.5 billion. Voice disorders are complex and multi-faceted, as well as difficult to diagnose and treat. Prospective animal studies are necessary to study the pathophysiology of voice disorders, optimize our understanding of laryngeal pathology, and improve treatment outcomes. The pig provides a unique opportunity to test hypotheses relating to laryngeal disease because porcine vocal …


The Pseudorabies Virus Protein, Pul56, Enhances Virus Dissemination And Virulence But Is Dispensable For Axonal Transport, Gina R. Daniel, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith Jan 2016

The Pseudorabies Virus Protein, Pul56, Enhances Virus Dissemination And Virulence But Is Dispensable For Axonal Transport, Gina R. Daniel, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

Neurotropic herpesviruses exit the peripheral nervous system and return to exposed body surfaces following reactivation from latency. The pUS9 protein is a critical viral effector of the anterograde axonal transport that underlies this process. We recently reported that while pUS9 increases the frequency of sorting of newly assembled pseudorabies virus particles to axons from the neural soma during egress, subsequent axonal transport of individual virus particles occurs with wild-type kinetics in the absence of the protein. Here, we examine the role of a related pseudorabies virus protein, pUL56, during neuronal infection. The findings indicate that pUL56 is a virulence factor …