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2015

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Articles 61 - 90 of 333

Full-Text Articles in Veterinary Medicine

Trim21 Regulates Nmi-Ifi35 Complex-Mediated Inhibition Of Innate Antiviral Response, Anshuman Das, Phat X. Dinh, Asit K. Pattnaik Sep 2015

Trim21 Regulates Nmi-Ifi35 Complex-Mediated Inhibition Of Innate Antiviral Response, Anshuman Das, Phat X. Dinh, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

In this study, using an immunoprecipitation coupled with mass spectrometry approach, we have identified the E3 ubiquitin ligase Trim21 as an interacting partner of IFI35 and Nmi. We found that this interaction leads to K63-linked ubiquitination on K22 residue of Nmi, but not IFI35. Using domain deletion analysis, we found that the interaction is mediated via the coiled-coil domain of Nmi and the carboxyl-terminal SPRY domain of Trim21. Furthermore, we show that depletion of Trim21 leads to significantly reduced interaction of Nmi with IFI35, which results in the abrogation of the negative regulatory function of the Nmi-IFI35 complex on innate …


Comparison Of The Efficacy Of Firocoxib And Carprofen In Clinical Use For Canine Coxofemoral Osteoarthritis, Nattawan Tangmahakul, Kumpanart Soontornvipart, Korakot Nganvongpanit Sep 2015

Comparison Of The Efficacy Of Firocoxib And Carprofen In Clinical Use For Canine Coxofemoral Osteoarthritis, Nattawan Tangmahakul, Kumpanart Soontornvipart, Korakot Nganvongpanit

The Thai Journal of Veterinary Medicine

Firocoxib and carprofen, non-steroidal anti-inflammatory drugs (NSAIDs), are used in the treatment of canine osteoarthritis. This study evaluated the efficacy and provided information of these NSAIDs in clinical practice. Sixteen healthy dogs of large breeds over 5 years old without systemic diseases and pregnancy that had coxofemoral osteoarthritis were divided into 2 groups, firocoxib group (n=9) and carprofen group (n=7). The study was conducted for 16 weeks. Firocoxib (5mg/kg body weight) or carprofen (4.4mg/kg body weight) was administered to all dogs once daily for 2 weeks, on alternate days for 6 weeks and every 2 days for 8 weeks. Of …


Genomic Characterization Of A New Tembusu Flavivirus From Domestic Ducks In Thailand, Warunya Chakritbudsabong, Jarupha Taowan, Preeda Lertwatcharasarakul, Sakuna Phattanakunanan, Angkasiya Munkhong, Thaweesak Songserm, Kridsada Chaichoun Sep 2015

Genomic Characterization Of A New Tembusu Flavivirus From Domestic Ducks In Thailand, Warunya Chakritbudsabong, Jarupha Taowan, Preeda Lertwatcharasarakul, Sakuna Phattanakunanan, Angkasiya Munkhong, Thaweesak Songserm, Kridsada Chaichoun

The Thai Journal of Veterinary Medicine

Tembusu virus (TMUV) namely KPS54A61 has spread in duck farms in many provinces in Thailand since 2010. The morbidity ranges from 15-30% and the mortality ranges from 10-15%. All infected ducks exhibit nervous signs including ataxia and paralysis. TMUV was successfully isolated from the brain and spinal cord of infected ducks by using C6/36 and Vero cells, but the typical cytopathic effect (CPE) was presented in the Vero cells only. Phylogenetic analysis of the whole genomic sequence of our TMUV-KPS54A61 virus showed that it belonged to the Ntaya virus group of Flaviviridae and its genomic sequence was similar to that …


Development Of An Indirect Elisa, Blocking Elisa, Fluorescent Microsphere Immunoassay And Fluorescent Focus Neutralization Assay For Serologic Evaluation Of Exposure To North American Strains Of Porcine Epidemic Diarrhea Virus, Faten Okda, Xiaodong Liu, Aaron Singrey, Travis Clement, Julie Nelson, Jane Christopher-Hennings, Eric A. Nelson, Steven Lawson Aug 2015

Development Of An Indirect Elisa, Blocking Elisa, Fluorescent Microsphere Immunoassay And Fluorescent Focus Neutralization Assay For Serologic Evaluation Of Exposure To North American Strains Of Porcine Epidemic Diarrhea Virus, Faten Okda, Xiaodong Liu, Aaron Singrey, Travis Clement, Julie Nelson, Jane Christopher-Hennings, Eric A. Nelson, Steven Lawson

Veterinary and Biomedical Sciences Faculty Publications

Recent, severe outbreaks of porcine epidemic diarrhea virus (PEDV) in Asia and North America highlight the need for well-validated diagnostic tests for the identification of PEDV infected animals and evaluation of their immune status to this virus. PEDV was first detected in the U.S. in May 2013 and spread rapidly across the country. Some serological assays for PEDV have been previously described, but few were readily available in the U.S. Several U.S. laboratories quickly developed indirect fluorescent antibody (IFA) assays for the detection of antibodies to PEDV in swine serum, indicating prior exposure. However, the IFA has several disadvantages, including …


Variation In Brain Morphology Of Intertidal Gobies: A Comparison Of Methodologies Used To Quantitatively Assess Brain Volumes In Fish, Gemma E. White, Culum Brown Aug 2015

Variation In Brain Morphology Of Intertidal Gobies: A Comparison Of Methodologies Used To Quantitatively Assess Brain Volumes In Fish, Gemma E. White, Culum Brown

Veterinary Science and Medicine Collection

When correlating brain size and structure with behavioural and environmental characteristics, a range of techniques can be utilised. This study used gobiid fishes to quantitatively compare brain volumes obtained via three different methods; these included the commonly used techniques of histology and approximating brain volume to an idealised ellipsoid, and the recently established technique of X-ray micro-computed tomography (micro-CT). It was found that all three methods differed significantly from one another in their volume estimates for most brain lobes. The ellipsoid method was prone to over- or under-estimation of lobe size, histology caused shrinkage in the telencephalon, and although micro-CT …


Prediction Of Parturition In Holstein Dairy Cattle Using Electronic Data Loggers, M. Titler, M. G. Maquivar, S. Bas, P. J. Rajala-Schultz, E. Gordon, K. Mccullough, P. Federico, G. M. Schuenemann Aug 2015

Prediction Of Parturition In Holstein Dairy Cattle Using Electronic Data Loggers, M. Titler, M. G. Maquivar, S. Bas, P. J. Rajala-Schultz, E. Gordon, K. Mccullough, P. Federico, G. M. Schuenemann

Physiology Collection

The objective of the present study was to assess the effect of parturition on behavioral activity [steps, standing time, lying time, lying bouts (LB), and duration of LB] 4 d before calving using electronic data loggers. Animals (n = 132) from 3 herds were housed in similar freestall barns using a prepartum pen 21 d before the expected calving date and were moved into a contiguous individual maternity pen for parturition. Electronic data loggers were placed on a hind leg of prepartum heifers (heifers, n = 33) and cows (cows, n = 99) at 7 ± 3 d before the …


Beef Cattle Management Systems For Estrus Synchronization And Heifer Development, Hazy Rae Nielson Aug 2015

Beef Cattle Management Systems For Estrus Synchronization And Heifer Development, Hazy Rae Nielson

Department of Animal Science: Dissertations, Theses, and Student Research

Four experiments were conducted to evaluate estrus synchronization and heifer development systems. In the first experiment delaying AI 19 h following GnRH administration in a hybrid estrus detection and time AI protocol was evaluated. Final pregnancy rate was not different among heifers detected in estrus, AI at the time GnRH administration, or delayed AI. The second study compared the effect of melengestrol acetate (MGA) -PG and 14-d controlled internal drug release (CIDR) -PG estrus synchronization protocols on estrus response and pregnancy rates of 311 d old heifers. Final pregnancy rate was similar between CIDR and MGA treatment groups. The objective …


The Cestode Community In Northern Fur Seals (Callorhinus Ursinus) On St. Paul Island, Alaska, Tetiana A Kuzmina, Jesús S. Hernández-Orts, Eugene T. Lyons, Terry R Spraker, Vadym V. Kornyushyn, Roman Kuchta Aug 2015

The Cestode Community In Northern Fur Seals (Callorhinus Ursinus) On St. Paul Island, Alaska, Tetiana A Kuzmina, Jesús S. Hernández-Orts, Eugene T. Lyons, Terry R Spraker, Vadym V. Kornyushyn, Roman Kuchta

Veterinary Science Faculty Publications

The diversity and ecology of cestodes from the northern fur seals, Callorhinus ursinus (NFS), were examined using newly collected material from 756 humanely harvested subadult males between 2011 and 2014. NFSs were collected from five different haul-outs on St. Paul Island, Alaska. A total of 14,660 tapeworms were collected with a prevalence of 98.5% and intensity up to 107 cestodes per host (mean intensity 19.7 ± 16.5 SD). Three species of tapeworms were found: Adenocephalus pacificus (Diphyllobothriidea) was the most prevalent (prevalence 97.4%), followed by Diplogonoporus tetrapterus (49.7%), and 5 immature specimens of Anophryocephalus cf. ochotensis (Tetrabothriidea) (0.5%). Most of …


Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem Jul 2015

Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

There is an urgent need for new antibiotics and alternative strategies to combat multidrug-resistant bacterial pathogens, which are a growing clinical issue. Repurposing existing approved drugs with known pharmacology and toxicology is an alternative strategy to accelerate antimicrobial research and development. In this study, we show that celecoxib, a marketed inhibitor of cyclooxygenase-2, exhibits broad-spectrum antimicrobial activity against Gram-positive pathogens from a variety of genera, including Staphylococcus, Streptococcus, Listeria, Bacillus, and Mycobacterium, but not against Gram-negative pathogens. However, celecoxib is active against all of the Gram-negative bacteria tested, including strains of, Acinetobacter, and Pseudomonas, when …


Diffusion Tensor Imaging Of Dolphin Brains Reveals Direct Auditory Pathway To Temporal Lobe, Gregory S. Berns, Peter F. Cook, Sean Foxley, Saad Jbabdi, Karla L. Miller, Lori Marino Jul 2015

Diffusion Tensor Imaging Of Dolphin Brains Reveals Direct Auditory Pathway To Temporal Lobe, Gregory S. Berns, Peter F. Cook, Sean Foxley, Saad Jbabdi, Karla L. Miller, Lori Marino

Veterinary Science and Medicine Collection

The brains of odontocetes (toothed whales) look grossly different from their terrestrial relatives. Because of their adaptation to the aquatic environment and their reliance on echolocation, the odontocetes’ auditory system is both unique and crucial to their survival. Yet, scant data exist about the functional organization of the cetacean auditory system. A predominant hypothesis is that the primary auditory cortex lies in the suprasylvian gyrus along the vertex of the hemispheres, with this position induced by expansion of ‘associative0 regions in lateral and caudal directions. However, the precise location of the auditory cortex and its connections are still unknown. Here, …


Epidemiological Associations Between Brachycephaly And Upper Respiratory Tract Disorders In Dogs Attending Veterinary Practices In England, Dan G. O'Neill, Caitlin Jackson, Jonathan H. Guy, David B. Church, Paul D. Mcgreevy, Peter Thomson, Dave C. Brodbelt Jul 2015

Epidemiological Associations Between Brachycephaly And Upper Respiratory Tract Disorders In Dogs Attending Veterinary Practices In England, Dan G. O'Neill, Caitlin Jackson, Jonathan H. Guy, David B. Church, Paul D. Mcgreevy, Peter Thomson, Dave C. Brodbelt

Epidemiology Collection

Background: Brachycephalic dog breeds are increasingly common. Canine brachycephaly has been associated with upper respiratory tract (URT) disorders but reliable prevalence data remain lacking. Using primary-care veterinary clinical data, this study aimed to report the prevalence and breed-type risk factors for URT disorders in dogs. Results: The sampling frame included 170,812 dogs attending 96 primary-care veterinary clinics participating within the VetCompass Programme. Two hundred dogs were randomly selected from each of three extreme brachycephalic breed types (Bulldog, French Bulldog and Pug) and three common small-to medium sized breed types (moderate brachycephalic: Yorkshire Terrier and non-brachycephalic: Border Terrier and West Highland …


Growing South Dakota (Summer 2015), College Of Agriculture &. Biological Sciences Jul 2015

Growing South Dakota (Summer 2015), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Exciting New Era Underway: New Facilities & New Degree Offerings Coming To Fruition
[Page] 5 USDA Report Touts Agriculture Among Best Fields For College Grads
[Page] 6 Critical Need: Essential Services Of ADRDL Need Support For Modernized Facility
[Page] 8 Quarterly Colloquium: State's Ag Leaders Collaborate
[Page] 9 Profiles In Leadership: Developing A Museum To Enrich And Transform Lives
[Page] 10 Productive Partnerships: Collaboration Between Producers & Researchers Guides Research Farm Efforts
[Page] 12 Coordinated Effort: SDSU's John Ball Serves Dual Role To Benefit South Dakota's Trees
[Page] 14 Progressive Perspective: Dan Oedekoven Reflects On Extension's Evolution
[Page] …


An Evaluation Of Porcine Epidemic Diarrhea Virus Survival In Individual Feed Ingredients In The Presence Or Absence Of A Liquid Antimicrobial, Scott Dee, Casey Neill, Travis Clement, Aaron Singrey, Jane Christopher-Hennings, Eric A. Nelson Jul 2015

An Evaluation Of Porcine Epidemic Diarrhea Virus Survival In Individual Feed Ingredients In The Presence Or Absence Of A Liquid Antimicrobial, Scott Dee, Casey Neill, Travis Clement, Aaron Singrey, Jane Christopher-Hennings, Eric A. Nelson

Veterinary and Biomedical Sciences Faculty Publications

Background: Contaminated complete feed and porcine plasma are risk factors for PEDV introduction to farms and a liquid antimicrobial has been proven useful for reducing risk. This study provides information on the survivability of PEDV across common swine feed ingredients in the presence or absence of the liquid antimicrobial.
Results: Eighteen ingredients commonly included in commercial swine diets were selected, including 3 grain sources (corn, soybean meal (SBM), dried distillers grains with solubles (DDGS)), 5 porcine by-products (spray-dried plasma, purified plasma, intestinal mucosa, meat and bone meal and red blood cells (RBCs)), 3 vitamin/trace mineral (VTM) mixes (sow, nursery, finishing), …


Changes In Serum Strongylus Vulgaris-Specific Antibody Concentrations In Response To Anthelmintic Treatment Of Experimentally Infected Foals, Martin Krarup Nielsen, Jessica Scare, Holli Sullivan Gravatte, Jennifer Lynn Bellaw, Julio C. Prado, Craig Robert Reinemeyer Jul 2015

Changes In Serum Strongylus Vulgaris-Specific Antibody Concentrations In Response To Anthelmintic Treatment Of Experimentally Infected Foals, Martin Krarup Nielsen, Jessica Scare, Holli Sullivan Gravatte, Jennifer Lynn Bellaw, Julio C. Prado, Craig Robert Reinemeyer

Maxwell H. Gluck Equine Research Center Faculty Publications

Strongylus vulgaris is the most pathogenic nematode parasite of horses. Its extensive migration in the mesenteric blood vessels can lead to life-threatening intestinal infarctions. Recent work has shown that this parasite is still identified among managed horse populations. A serum enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of migrating larvae of S. vulgaris. Previous work has documented an increase in ELISA values following larvicidal treatment with ivermectin and suggested that the target parasite antigen is primarily produced by the later larval stages. The aim of this study was to experimentally inoculate cohorts of foals with S. …


Annotation Of The Protein Coding Regions Of The Equine Genome, Matthew S. Hestand, Theodore S. Kalbfleisch, Stephen J. Coleman, Zheng Zeng, Jinze Liu, Ludovic Orlando, James N. Macleod Jun 2015

Annotation Of The Protein Coding Regions Of The Equine Genome, Matthew S. Hestand, Theodore S. Kalbfleisch, Stephen J. Coleman, Zheng Zeng, Jinze Liu, Ludovic Orlando, James N. Macleod

Veterinary Science Faculty Publications

Current gene annotation of the horse genome is largely derived from in silico predictions and cross-species alignments. Only a small number of genes are annotated based on equine EST and mRNA sequences. To expand the number of equine genes annotated from equine experimental evidence, we sequenced mRNA from a pool of forty-three different tissues. From these, we derived the structures of 68,594 transcripts. In addition, we identified 301,829 positions with SNPs or small indels within these transcripts relative to EquCab2. Interestingly, 780 variants extend the open reading frame of the transcript and appear to be small errors in the equine …


Comparison Of The Equine Reference Sequence With Its Sanger Source Data And New Illumina Reads, Jovan Rebolledo-Mendez, Matthew S. Hestand, Stephen J. Coleman, Zheng Zeng, Ludovic Orlando, James N. Macleod, Ted Kalbfleisch Jun 2015

Comparison Of The Equine Reference Sequence With Its Sanger Source Data And New Illumina Reads, Jovan Rebolledo-Mendez, Matthew S. Hestand, Stephen J. Coleman, Zheng Zeng, Ludovic Orlando, James N. Macleod, Ted Kalbfleisch

Maxwell H. Gluck Equine Research Center Faculty Publications

The reference assembly for the domestic horse, EquCab2, published in 2009, was built using approximately 30 million Sanger reads from a Thoroughbred mare named Twilight. Contiguity in the assembly was facilitated using nearly 315 thousand BAC end sequences from Twilight's half brother Bravo. Since then, it has served as the foundation for many genome-wide analyses that include not only the modern horse, but ancient horses and other equid species as well. As data mapped to this reference has accumulated, consistent variation between mapped datasets and the reference, in terms of regions with no read coverage, single nucleotide variants, and small …


In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, Kyong-Sup Yoon, Jennifer K. Ketzis, Samuel W. Andrewes, Christopher S. Wu, Kris Honraet, Dorien Staljanssens, Bart Rossel, J Marshall Clark Jun 2015

In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, Kyong-Sup Yoon, Jennifer K. Ketzis, Samuel W. Andrewes, Christopher S. Wu, Kris Honraet, Dorien Staljanssens, Bart Rossel, J Marshall Clark

SIUE Faculty Research, Scholarship, and Creative Activity

The human head louse is a cosmopolitan ectoparasite and frequently infests many people, particularly school-age children. Due to widespread pyrethroid resistance and the lack of efficient resistance management, there has been a considerable interest in the protection of uninfested people and prevention of reinfestation by disrupting lice transfer. In this study, two nonclinical model systems (in vitro and in vivo) were used to determine the efficacy of the infestation deterrents, Elimax lotion and Elimax shampoo, against human head lice or poultry chewing lice, respectively. With in vitro assessments, female head lice exhibited significantly higher avoidance responses to hair tufts treated …


Animal Health Matters, Russ Daly, Jane Hennings Jun 2015

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- ADRDL Steps Up in Light of Highly Pathogenic H5N2 Avian Influenza Outbreaks [Page] 2- Director's Message: The SD Animal Disease Research and Diagnostic Laboratory and Emergency Testing for Highly Pathogenic Avian Influenza [Page] 3- Pre-Veterinary Students Honored by SDVMA and SDSU Through Stethoscope Ceremony [Page] 4- Clerical Staff Makes Strides in Efficiency and Service [Page] 5- ADRDL Pathologists Team with Veterinarian to Address Cattle Lameness for Livestock Group [Page] 5- Canine Influenza Testing—SDSU ADRDL [Page] 6- ADRDL Hosts Tours for Legislators and State Agency Staff [Page] 6- ADRDL Offers Next Generation Sequencing [Page] 6- ADRDL at the SDVMA …


Effect Of Pcso-524 On Oa Biomarkers And Weight-Bearing Properties In Canine Shoulder And Coxofemeral Osteoarthritis, Kumpanart Soontornvipart, Natwadee Mongkhon, Korakot Nganvongpanit, Prachya Kongtawelert Jun 2015

Effect Of Pcso-524 On Oa Biomarkers And Weight-Bearing Properties In Canine Shoulder And Coxofemeral Osteoarthritis, Kumpanart Soontornvipart, Natwadee Mongkhon, Korakot Nganvongpanit, Prachya Kongtawelert

The Thai Journal of Veterinary Medicine

This study was designed to compare the therapeutic benefits of a compound of omega-3 fatty acids from the New Zealand green-lipped mussel (Perna canaliculus) (PCSO-524) and omega-3 fatty acids in fish oil on clinical outcomes and osteoarthritis biomarkers (chondroitin sulfate WF6 epitope) in 66 dogs that had osteoarthritis (OA); 39 dogs with OA hip joints, 15 dogs with OA shoulder joints and 12 dogs with OA shoulder and hip joints. The animals were presented at the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University. The dogs were allocated into two groups randomly. One group received PCSO-524 (n = 33) …


Use Of Essential Oils For Manipulation Of Rumen Microbial Fermentation Using Batch Culture, Atitthan Nanon, Wisitiporn Suksombat, Zhu Yang Wen Jun 2015

Use Of Essential Oils For Manipulation Of Rumen Microbial Fermentation Using Batch Culture, Atitthan Nanon, Wisitiporn Suksombat, Zhu Yang Wen

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the effects of various levels of essential oils on feed digestibility, gas production and rumen fermentation. Two batch cultures were designed for screening various doses of each essential oil (EO). Treatments were control (CON), cinnamon oil (CIN), clove oil (CLO), garlic oil (GAR), ginger oil (GIN), and lemongrass oil (LEM). Dosages were 0, 200, 400, 800, and 1600 mg/kg DM in experiment I (1st batch) and 50, 100, 150, and 200 mg/kg DM in experiment II (2nd batch). Digestibility of DM (DMD), neutral detergent fiber and acid detergent fiber was measured at …


Infection Of Thai Influenza A Viruses Subtype H5n1 Using Tracheal Culture, Napawan Bunpapong, Supanat Boonyapisitsopa, Kamol Suwannakarn, Rachod Tantilertchareon, Roongroje Thanawongnuwech, Alongkorn Amonsin Jun 2015

Infection Of Thai Influenza A Viruses Subtype H5n1 Using Tracheal Culture, Napawan Bunpapong, Supanat Boonyapisitsopa, Kamol Suwannakarn, Rachod Tantilertchareon, Roongroje Thanawongnuwech, Alongkorn Amonsin

The Thai Journal of Veterinary Medicine

Avian influenza (AI) is caused by influenza A virus subtype H5N1. The virus causes severe disease and death in several avian and mammalian species. This study investigated the ability of swine and chicken tracheal cultures to support highly pathogenic avian influenza subtype H5N1 (HPAI-H5N1) viral replication. Three Thai HPAI-H5N1 viruses isolated from chicken (A/chicken/Thailand/CU-K2/04), duck (A/duck/Thailand/CU-328/07), and tiger (A/tiger/Thailand /CU-T7/04) were used to infect the swine and chicken tracheal cultures. Our results indicated that the chicken tracheal culture could support virus replication in contrast to the swine tracheal culture, suggesting that pig has low susceptibility to Thai HPAI-H5N1 infection. Immunohistochemical …


Effects Of Vitamin D3 And Calcium On Productive Performance, Egg Quality And Vitamin D3 Content In Egg Of Second Production Cycle Hens, Hatairat Plaimast, Suwanna Kijparkorn, Punyaphat Ittitanawong Jun 2015

Effects Of Vitamin D3 And Calcium On Productive Performance, Egg Quality And Vitamin D3 Content In Egg Of Second Production Cycle Hens, Hatairat Plaimast, Suwanna Kijparkorn, Punyaphat Ittitanawong

The Thai Journal of Veterinary Medicine

This experiment was conducted to investigate the effect of supplementary vitamin D3 on productive performance, internal egg quality, eggshell quality, apparent calcium digestibility and vitamin D3 content in egg of second production cycle hens fed on different levels of calcium. A total of 140 second cycle brown-egg laying hens, aged 98 weeks, were randomly divided into 5 treatments with 4 replicates. The treatments consisted of diets containing 3.5%Ca and 2,000 IU of vitamin D3/kg of feed (control), 3.5%Ca and 6,000 IU of vitamin D3/kg of feed (treatment 2), 3.75%Ca and 2,000 IU of vitamin D3/kg of feed (treatment 3), 3.75%Ca …


Protection Against Mycoplasma Gallisepticum In Layers Immunized With Recombinant Fowl Poxvirus Vaccine Followed By Live F Strain Vaccine, Somsak Pakpinyo, Arithat Limsatanun, Kreangkrai Sangthongdang, Marcelo Paniago, Roberto Soares Jun 2015

Protection Against Mycoplasma Gallisepticum In Layers Immunized With Recombinant Fowl Poxvirus Vaccine Followed By Live F Strain Vaccine, Somsak Pakpinyo, Arithat Limsatanun, Kreangkrai Sangthongdang, Marcelo Paniago, Roberto Soares

The Thai Journal of Veterinary Medicine

Generally, one administration of either a recombinant fowl poxvirus-Mycoplasma gallisepticum (rFPV-MG) or a live F strain (FMG) vaccine is recommended in layer flocks. This study aimed to assess MG protection when the rFPV-MG and FMG vaccines were given to layers as opposed to a single vaccination with FMG vaccine. One hundred 8-week-old layer chickens were divided into 4 groups as follows: group 1 received FMG at 11 weeks of age, group 2 received rFPV-MG at 8 weeks of age and FMG at 11 weeks of age, groups 3 and 4 served as positive and negative controls, respectively. At 14 weeks …


Analysis Of Virulence Genes And Pathogenicity Of Thai Mycoplasma Gallisepticum Isolates, Pacharaporn Khumpim, Rapeewan Tampaisarn, Jiroj Sasipreeyajan, Somsak Pakpinyo Jun 2015

Analysis Of Virulence Genes And Pathogenicity Of Thai Mycoplasma Gallisepticum Isolates, Pacharaporn Khumpim, Rapeewan Tampaisarn, Jiroj Sasipreeyajan, Somsak Pakpinyo

The Thai Journal of Veterinary Medicine

Mycoplasma gallisepticum (MG) causes chronic respiratory disease in poultry. MG organisms consist of several virulence genes which are related to infection. The aim of this study was to analyze the virulence genes and determine the pathogenicity of Thai MG isolates. In this study, there were 3 experiments; nineteen MG isolates; and 2 reference strains, F and S6 strains, analyzed to detect 4 virulence genes (LP, gapA, pvpA and mgc2) by PCR assay (experiment 1). The pathogenicity study of sham negative control group, S6 strain, 3 selected isolates (AHRL 31/46, 54/46 and 58/46) and F strain, respectively, were inoculated into 6 …


Genetic Correlations Among Average Daily Gain, Backfat Thickness And Sow Longevity In Landrace And Yorkshire Sows, Nuttha Wongsakajornkit, Nalinee Imboonta Jun 2015

Genetic Correlations Among Average Daily Gain, Backfat Thickness And Sow Longevity In Landrace And Yorkshire Sows, Nuttha Wongsakajornkit, Nalinee Imboonta

The Thai Journal of Veterinary Medicine

The present study aimed to estimate heritabilities as well as genetic correlations of sow longevity with average daily gain and backfat thickness. Removed sows included in the current study were 1,706 Landrace sows and 1,159 Yorkshire sows. They were raised in a Thai swine breeding herd and removed in the 2008-2013 period. Sow longevity was defined as the number of days from first farrowing date to removal date. Genetic parameters were estimated by using the Restricted Maximum Likelihood (REML) method with a multiple-trait animal model. On average, the sow longevity, average daily gain and backfat thickness were 633 days, 863 …


Effect Of Various Combinations Of Sugar Supplementation In The Extender On Frozen-Thawed Ram Semen Quality And Fertility, Saritvich Panyaboriban, Junpen Suwimonteerabutr, Nawapen Phutikanit, Theerawat Swangchan-Uthai, Theerawat Tharasanit, Mongkol Techakumphu Jun 2015

Effect Of Various Combinations Of Sugar Supplementation In The Extender On Frozen-Thawed Ram Semen Quality And Fertility, Saritvich Panyaboriban, Junpen Suwimonteerabutr, Nawapen Phutikanit, Theerawat Swangchan-Uthai, Theerawat Tharasanit, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

Semen cryopreservation alters sperm structure and biochemical properties, and subsequently impairs fertilizing ability. A combination of two different sugar molecules revealed an enhanced beneficial effect on frozen-thawed spermatozoa of various species. However, the report in ram has been scarce. The present study investigated the effect of different combinations of sugar supplementation on post-thawed ram semen qualities in a cryopreservative extender in the presence of a single sugar or a combination of sugars. Data showed that a combination of sucrose and trehalose significantly improved cryopreserved sperm motility, viability, longevity and acrosome integrity. Fertility rates following laparoscopic artificial insemination (LAI) using frozen-thawed …


Electrolyte And Ascorbic Acid Supplementation For Heat Exposed Broilers In Thailand, Chackrit Nuengjamnong, Kris Angkanaporn Jun 2015

Electrolyte And Ascorbic Acid Supplementation For Heat Exposed Broilers In Thailand, Chackrit Nuengjamnong, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

The objective of this study was to compare the effects of ascorbic acid and mixed electrolytes on growth performance, ammonia emission and serum antioxidant enzyme activity in broilers during summer period. Two hundred and ten male and female Arbor Acre broilers during grower-finisher period (22-42 days old) were allocated into 3 groups with different additives in drinking water: 1,000 ppm ascorbic acid (T1); 2,000 ppm mixed electrolytes containing only Na+, K+ and HCO3- (T2) and none (T3 serving as control group). The experiment was conducted in an open broiler house during the summer period between April and May. Results indicated …


Efficacy Of Salinomycin, Robenidine And Decoquinate Against Infection With Eimeria Species Field Isolate In A Densely Populated Broiler Farm In Thailand, Morakot Kaewthamasorn, Nataya Charoenvisal, Niwat Chansiripornchai Jun 2015

Efficacy Of Salinomycin, Robenidine And Decoquinate Against Infection With Eimeria Species Field Isolate In A Densely Populated Broiler Farm In Thailand, Morakot Kaewthamasorn, Nataya Charoenvisal, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Avian coccidiosis is caused by intracellular protozoan parasites in the genus Eimeria. It has a significant economic impact on the poultry industry and is highly prevalent in chicken producing countries worldwide. Current control measurements including good animal husbandry, chemoprophylaxis, and vaccination cannot effectively control the disease. It is well understood that parasites repeatedly exposed to the same anticoccidial drug is a major cause of resistance. Therefore, it is critical to monitor parasite response to anticoccidial drugs in different field settings. The aim of this experiment was to investigate responses of a local field isolate of Eimeria species to commonly used …


Inhibitory Effects Of Aluminosilicates On Lead Acetate Toxicity In Selected Organs Of Broilers, Dejan Prvulović, Danijela Kojić, Milan Popović, Gordana Grubor-Lajšić Jun 2015

Inhibitory Effects Of Aluminosilicates On Lead Acetate Toxicity In Selected Organs Of Broilers, Dejan Prvulović, Danijela Kojić, Milan Popović, Gordana Grubor-Lajšić

The Thai Journal of Veterinary Medicine

The aim of this study was to determine the effect of dietary supplements of lead acetate and aluminosilicates on oxidative status in brain, kidney and duodenum of broiler chickens. In this regard, activity of antioxidative enzymes such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPx), pyrogallol peroxidase (PPx) and lipid peroxidation was determined in examined organs. The experiment was performed with eighty-four broiler chickens of both sexes randomly allotted to four diets including the control group, the Pb group containing 500 mg lead acetate/kg diet, the ATN (Antitoxic nutrient) group with 5 g mixture of aluminosilicates (zeolite and montmorillonite)/kg …


Extended Spectrum Beta-Lactamase Producing Escherichia Coli Isolated From Infected Canines, Parama Hanhaboon, Thachawet Kimprasit, Worrawit Wajjwalku, Patamabhorn Amavisit Jun 2015

Extended Spectrum Beta-Lactamase Producing Escherichia Coli Isolated From Infected Canines, Parama Hanhaboon, Thachawet Kimprasit, Worrawit Wajjwalku, Patamabhorn Amavisit

The Thai Journal of Veterinary Medicine

Escherichia coli strains from dogs with different clinical signs were studied for their antimicrobial sensitivity profiles, extended spectrum beta-lactamase enzymes, and virulent genes. The 364 E. coli isolates originated from the skin (32.48%), urinary tract (31.32%), gastrointestinal tract (16.21%), reproductive system (7.69%), ear (6.05%), respiratory tract (4.94%), and other specimens (1.37%). Using the disk diffusion method, the E. coli were resistant to amoxicillin, amoxicillin-clavulanic acid, azithromycin, cephalexin, ceftriaxone, ciprofloxacin, doxycycline, enrofloxacin, norfloxacin, gentamicin, and sulfamethoxazole/trimethoprim at the rates of 84, 36, 41, 57, 38, 66, 73, 70, 64, 47, and 64%, respectively. Ninety-seven E. coli that were resistant to two …