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Veterinary Medicine Commons™

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2015

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Articles 211 - 240 of 333

Full-Text Articles in Veterinary Medicine

Characterization Of Newly Revealed Sequences In The Infectious Myonecrosis Virus Genome In Litopenaeus Vannamei, Duan S. Loy, Sijun Liu, Mark A. Mogler, J. Dustin Loy, Bradley J. Blitvich, Lyric C. Bartholomay Jan 2015

Characterization Of Newly Revealed Sequences In The Infectious Myonecrosis Virus Genome In Litopenaeus Vannamei, Duan S. Loy, Sijun Liu, Mark A. Mogler, J. Dustin Loy, Bradley J. Blitvich, Lyric C. Bartholomay

School of Veterinary and Biomedical Sciences: Faculty Publications

Infectious myonecrosis virus (IMNV) causes significant economic losses in farmed shrimp, where associated mortality in ponds can reach 70%. To explore host/pathogen interactions, a next-generation sequencing approach using lymphoid organ tissue from IMNV-infected Litopenaeus vannamei shrimp was conducted. Preliminary sequence assembly of just the virus showed that there were at least an additional 639 bp at the 5′ terminus and 23 nt at the 3′ terminus as compared with the original description of the IMNV genome (7561 nt). Northern blot and reverse transcription-PCR analysis confirmed the presence of novel sequence at both ends of the genome. Using 5′ RACE, an …

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Mri-Compatible Bioreactors And Methods Of Using, Shadi Othman, Huihui Xu, Karin Wartella, Vahid Khalilzad-Sharghi, Ian Bargar Jan 2015

Mri-Compatible Bioreactors And Methods Of Using, Shadi Othman, Huihui Xu, Karin Wartella, Vahid Khalilzad-Sharghi, Ian Bargar

School of Veterinary and Biomedical Sciences: Faculty Publications

This disclosure describes a MRI-compatible bioreactor that allows a biological sample to be imaged in culture without disrupting or compromising the culture.

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Major Histocompatibility Complex Class Ii Dextramers: New Tools For The Detection Of Antigen-Specific, Cd4 T Cells In Basic And Clinical Research, Chandirasegara Massilamany, Bharathi Krishnan, Jay Reddy Jan 2015

Major Histocompatibility Complex Class Ii Dextramers: New Tools For The Detection Of Antigen-Specific, Cd4 T Cells In Basic And Clinical Research, Chandirasegara Massilamany, Bharathi Krishnan, Jay Reddy

Jay Reddy Publications

The advent of major histocompatibility complex (MHC) tetramer technology has been a major contribution to T cell immunology, because tetramer reagents permit detection of antigen-specific T cells at the single-cell level in heterogeneous populations by flow cytometry. However, unlike MHC class I tetramers, the utility of MHC class II tetramers has been less frequently reported. MHC class II tetramers can be used successfully to enumerate the frequencies of antigen-specific CD4 T cells in cells activated in vitro, but their use for ex vivo analyses continues to be a problem, due in part to their activation dependency for binding with T …

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Widespread Detection Of Antibodies To Leptospira In Feral Swine In The United States, K. K. Pedersen, K. L. Pabilonia, T. D. Anderson, S. N. Bevins, C. R. Hicks, J. M. Kloft, Thomas J. Deliberto Jan 2015

Widespread Detection Of Antibodies To Leptospira In Feral Swine In The United States, K. K. Pedersen, K. L. Pabilonia, T. D. Anderson, S. N. Bevins, C. R. Hicks, J. M. Kloft, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

As feral swine continue to expand their geographical range and distribution across the United States, their involvement in crop damage, livestock predation, and pathogen transmission is likely to increase. Despite the relatively recent discovery of feral swine involvement in the aetiology of a variety of pathogens, their propensity to transmit and carry a wide variety of pathogens is disconcerting. We examined sera from 2055 feral swine for antibody presence to six serovars of Leptospira that can also infect humans, livestock or domestic animals. About 13% of all samples tested positive for at least one serovar, suggesting that Leptospira infection is …

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Development And Characterization Of A Live-Attenuated Vaccine To Combat Equine Herpesvirus Type-1 Infections, Shiliang Anthony Liu Jan 2015

Development And Characterization Of A Live-Attenuated Vaccine To Combat Equine Herpesvirus Type-1 Infections, Shiliang Anthony Liu

LSU Doctoral Dissertations

Equine Herpesvirus 1 (EHV-1) is an important ubiquitous enzootic equine pathogen and one of the most common pathogens of the horse, causing, respiratory disease, epidemic abortion, occasionally neurological disease in horses, which leads to significant economic losses to the horse industry. EHV-1 induces several clinical signs of disease ranging in severity, from mild respiratory disease to abortion in pregnant mares, neonatal foal death and neuropathogenic disorders. Natural EHV-1 infection stimulated short lived protective immunity and had both humoral and cellular immune responses. Currently vaccination remains the best option to prevent EHV-1 infection and different applications of vaccination have been investigated …

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Reference Values For Selected Hematological And Biochemical Blood Parameters From Prepregnancy To Advanced Gestation In Angora Cats, Özkan Şi̇mşek, Şevket Arikan, Mi̇yase Çinar Jan 2015

Reference Values For Selected Hematological And Biochemical Blood Parameters From Prepregnancy To Advanced Gestation In Angora Cats, Özkan Şi̇mşek, Şevket Arikan, Mi̇yase Çinar

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to monitor some hematological and biochemical blood parameters from prepregnancy to advanced gestation in Angora cats. In the present study, 35 blood samples obtained before and during pregnancy from 7 adult cats were used. Blood was collected from cats before pregnancy and on days 15, 30, 45, and 55 of gestation. The blood samples were analyzed in terms of WBC, RBC, Hb, PCV, MCV, MCH, MCHC, GRA, MON, and LYM values. Additionally, serum ALT, AST, ALP, GGT, LDH, and CK activities and total cholesterol, glucose, triglyceride, urea, Ca, Mg, and Pi levels were measured. …

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Single Nucleotide Polymorphism Identification And Characterization Of Gnrhr Gene In Bos Taurus And Bos Indicus Cattle, Ramesha Kerekoppa, Marappa Basavaraju, Akhila Rao, Gujjar Ramachandra Geetha, Kataktalware Mukund, Jeyakumar Sakthivel Jan 2015

Single Nucleotide Polymorphism Identification And Characterization Of Gnrhr Gene In Bos Taurus And Bos Indicus Cattle, Ramesha Kerekoppa, Marappa Basavaraju, Akhila Rao, Gujjar Ramachandra Geetha, Kataktalware Mukund, Jeyakumar Sakthivel

Turkish Journal of Veterinary & Animal Sciences

The hypothalamic gonadotropin-releasing hormone receptor (GnRHR) plays an essential physiological role in reproductive function. In this investigation, we studied genetic variation in the entire coding region of GnRHR gene using PCR-SSCP technique in 250 Bos taurus (Holstein Friesian, Jersey) and Bos indicus (Malnad Gidda, Deoni) bulls. The genetic variants in GnRHR gene were determined by PCR-SSCP technique by using 5 sets of primers. SSCP analysis of fragment 1, comprising exon 1, and fragment 3, comprising exon 2, revealed 2 SSCP patterns in all breeds under study, while fragment 2 of exon 1 and fragment 4, comprising exon 3, revealed 3 …

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Implementing Innovative Farm Management Practices On Dairy Farms: A Review Of Feeding Systems, Şeyda Özkan, Julian Hill Jan 2015

Implementing Innovative Farm Management Practices On Dairy Farms: A Review Of Feeding Systems, Şeyda Özkan, Julian Hill

Turkish Journal of Veterinary & Animal Sciences

The Australian dairy industry relies primarily on pasture for its feed supply. However, the variability in rainfall negatively affects plant growth, leading to uncertainty in dryland feed supply, especially during periods of high milk price. New feeding (complementary) systems combining perennial ryegrass with another crop and/or pasture species may have the potential to mitigate this seasonal risk and improve productivity and profitability by providing off-season feed. To date, the majority of research studying the integration of alternative crops into pasture-based systems has focused on substitution and utilization of alternative feed sources. There has been little emphasis on the impacts of …

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Endometrial Cytology As A Diagnostic Tool For Subclinical Endometritis In Beef Heifers, Carlos Moscuzza, Guadalupe Alvarez, Betiana Gutierrez, Marcelo Zurita, Marcelo Tropeano, Roberto Perna Jan 2015

Endometrial Cytology As A Diagnostic Tool For Subclinical Endometritis In Beef Heifers, Carlos Moscuzza, Guadalupe Alvarez, Betiana Gutierrez, Marcelo Zurita, Marcelo Tropeano, Roberto Perna

Turkish Journal of Veterinary & Animal Sciences

The obstetric assistance of dystocia in field conditions promotes greater bacterial contamination of the uterus, causing subclinical endometritis. The aim of this study was to determine the occurrence of subclinical endometritis in beef heifers receiving professional calving assistance, and to evaluate endometrial cytology as a diagnostic technique compared to uterine biopsy. A group of 829 Angus heifers were assisted at calving. Dystocia was classified based on difficulty at assistance, which was estimated as complex or simple. At the end of the calving season, 93 dystocic heifers were reported (10.7%). The percentage of simple resolution and complex resolution was 88.2% and …

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Effects Of Alpha Lipoic Acid And Vitamin C Supplementation On Transforming Growth Factor Alpha (Tgf \Alpha) And Transforming Growth Factor Beta 2 (Tgf \Beta2)Secretion In Testicular Tissue Of Diabetic Rats, Sevda Eli̇ş Yildiz, Buket Bakir, Hami̇t Uslu, Ebru Karadağ Sari, Musa Karaman Jan 2015

Effects Of Alpha Lipoic Acid And Vitamin C Supplementation On Transforming Growth Factor Alpha (Tgf \Alpha) And Transforming Growth Factor Beta 2 (Tgf \Beta2)Secretion In Testicular Tissue Of Diabetic Rats, Sevda Eli̇ş Yildiz, Buket Bakir, Hami̇t Uslu, Ebru Karadağ Sari, Musa Karaman

Turkish Journal of Veterinary & Animal Sciences

The effects of alpha lipoic acid and vitamin C supplementation on TGF \alpha and TGF \beta2 secretion in the testicular tissue of streptozotocin-induced diabetic rats were examined by immunohistochemical methods. Rats were divided into 5 groups; group 1 (control), 2 (diabetic control), 3 (diabetes + alpha lipoic acid), 4 (diabetes + vitamin C), and 5 (diabetes + alpha lipoic acid + vitamin C). Hematoxylin and eosin staining was performed in order to examine testicular tissue histopathologically. The streptavidin-biotin-peroxidase complex method was used for histological examination and determination of TGF \alpha and TGF \beta2 localization in the testicular tissue. Regarding fasting …

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Incidence And Occurrence Time Of Clinical Mastitis In Holstein Cows, Ismail Boujenane, Jalila El Aimani, Khalid By Jan 2015

Incidence And Occurrence Time Of Clinical Mastitis In Holstein Cows, Ismail Boujenane, Jalila El Aimani, Khalid By

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to assess the incidence of clinical mastitis (CM) and its risk factors in 1725 Holstein cows. Data were collected from a private farm from 2008 to 2012. The analysis of risk factors, performed with logistic regression, showed that cows at parity 2, 3, and 4 had 65%, 88%, and 115% risk of mastitis, respectively. This risk was higher (P < 0.001) than in cows at first parity. Cows that calved from October to January had the highest (P < 0.05) risk of mastitis. The number of mastitis cases in cows at lactation 2, 3, and 4 was 18.5%, 25.9%, and 40.7%, respectively, and was higher compared to cows at first lactation. Furthermore, cows that calved from July to September showed the highest number of mastitis cases. Gamma regression parameter estimates showed that the onset time of mastitis 1 and 2 in cows that calved from October to January occurred at 16.3 and 8.5 days, respectively. This was later than cows calving from July to September. The study concluded that it is necessary to examine cows for CM during the first 2 months of lactation in order to prevent mastitis and decrease its rate of incidence.

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Determination Of Alpha-Naphthyl Acetate Esterase (Anae) Activity In Peripheral Blood Leukocytes Of Pregnant, Adult, And Kitten Angora Cats, Alev Gürol Bayraktaroğlu, Özkan Şi̇mşek, Aytül Kürüm, Şevket Arikan, Emel Ergün Jan 2015

Determination Of Alpha-Naphthyl Acetate Esterase (Anae) Activity In Peripheral Blood Leukocytes Of Pregnant, Adult, And Kitten Angora Cats, Alev Gürol Bayraktaroğlu, Özkan Şi̇mşek, Aytül Kürüm, Şevket Arikan, Emel Ergün

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the alpha-naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in pregnant, kitten, and adult Angora cats. In each group 9 healthy pregnant, adult, and kitten Angora cats constituted the material of the study. Optimum reaction was achieved after a 3 h incubation period at pH 5.8, by ANAE staining. Two types of reaction were observed in ANAE (+) T lymphocytes. The first reaction was a dot-like positivity pattern characterized by the presence of large granules, while the other was a granular positivity pattern characterized by the presence of 3 to 5 small granules. B …

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Histological And Histochemical Studies On The Structure Of Pancreatic Histological And Histochemical Studies On The Structure Of Pancreatic Ducts Of The Goose (Anser Anser), Turgay Deprem, Serap Koral Taşçi, Seyi̇t Ali̇ Bi̇ngöl, Ebru Karadağ Sari, Şahi̇n Aslan, Serap İlhan Jan 2015

Histological And Histochemical Studies On The Structure Of Pancreatic Histological And Histochemical Studies On The Structure Of Pancreatic Ducts Of The Goose (Anser Anser), Turgay Deprem, Serap Koral Taşçi, Seyi̇t Ali̇ Bi̇ngöl, Ebru Karadağ Sari, Şahi̇n Aslan, Serap İlhan

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine the histological structure and histochemical properties of the pancreatic ducts in the goose Anser anser. Pancreatic ducts of 6 male and 6 female geese were examined. Crossman’s triple stain and Masson’s trichrome stain were used for histological examination, and periodic acid–Schiff, alcian blue, periodic acid–Schiff/alcian blue, and aldehyde fuchsin/alcian blue stainings were performed for histochemical examination. Dorsal and ventral pancreatic ducts were found that transfer pancreatic secretion into the duodenum of the goose, and these channels were found to have a histologically similar structure. No differences were detected between male and …

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Effect Of Dietary Seaweed (Ulva Lactuca) Supplementation On Growth Performance Of Sheep And On In Vitro Gas Production Kinetics, Ahmed El-Waziry, Ahmed Al-Haidary, Aly Okab, Emad Samara, Khalid Abdoun Jan 2015

Effect Of Dietary Seaweed (Ulva Lactuca) Supplementation On Growth Performance Of Sheep And On In Vitro Gas Production Kinetics, Ahmed El-Waziry, Ahmed Al-Haidary, Aly Okab, Emad Samara, Khalid Abdoun

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the effect of dietary seaweed (Ulva lactuca) supplementation on growth performance of sheep, in vitro gas production, estimated energy, and microbial protein synthesis. A total of 18 Naimey male sheep with average live weight of 22.78 ± 0.24 kg were randomly allocated to 3 groups. Sheep in group 1 were fed a diet containing commercial feed without seaweed as a control diet, sheep in group 2 were fed the control diet with 3% seaweed, and sheep in group 3 were fed the control diet with 5% seaweed. The supplementation of seaweed had no …

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Effect Of Organic Zinc Supplementation On Growth, Nutrient Utilization, And Plasma Zinc Status In Lambs, Mokhtar Mallaki, Mohammad Ali Norouzian, Ali Akbar Khadem Jan 2015

Effect Of Organic Zinc Supplementation On Growth, Nutrient Utilization, And Plasma Zinc Status In Lambs, Mokhtar Mallaki, Mohammad Ali Norouzian, Ali Akbar Khadem

Turkish Journal of Veterinary & Animal Sciences

Two experiments were conducted to investigate the effect of supplemental zinc (Zn) on performance, nutrient digestibility, and plasma Zn status in Zandi lambs. In experiment 1, 18 male lambs (BW = 21.30 ± 0.55 kg) were fed a basal diet containing 22.8 mg Zn/kg dry matter (DM) with no supplemental Zn (control) or 20 mg of supplemental Zn/kg of DM from Zn sulfate (ZnS) or Zn peptide (ZnP). Average daily gain and dry matter intake were higher for the lambs fed the diet supplemented with ZnP. Feed conversion ratio was significantly (P < 0.05) lower in the ZnP group compared to the control and ZnS groups. In experiment 2, an in vitro gas production technique was used to evaluate the effects of zinc source on the gas production parameters. Rate of gas production after 6 h and total production of gas after 24 and 48 h did not differ between groups. In vitro DM digestibility, short-chain fatty acids, and ME content were increased (P < 0.05) by ZnP supplementation when compared to the ZnS and control groups. The results of this study showed that feeding ZnP improves performance and digestibility of DM and could result in higher metabolizable energy and short-chain fatty acid yield.

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Relationships Between Sarcomere Length And Basic Composition Of Infraspinatus And Longissimus Dorsi Muscle, Dominika Guzek, Dominika Glabska, Krzysztof Glabski, Grzegorz Pogorzelski, Jerzy Barszczewski, Agnieszka Wierzbicka Jan 2015

Relationships Between Sarcomere Length And Basic Composition Of Infraspinatus And Longissimus Dorsi Muscle, Dominika Guzek, Dominika Glabska, Krzysztof Glabski, Grzegorz Pogorzelski, Jerzy Barszczewski, Agnieszka Wierzbicka

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the relationship between sarcomere length and basic composition (water, protein, ash, collagen, and fat content including marbling) of beef muscles with fast-twitch glycolytic (longissimus dorsi muscle) and slow-twitch oxidative fibers (infraspinatus muscle) in Limousin bulls. Samples (n = 15) were obtained from federally inspected slaughter facilities, and animals used in the experiment were treated according to standard ethical norms. The shortest sarcomeres were observed for longissimus dorsi from striploin, and the longest for infraspinatus muscle. Additionally, the influence of both cut and animal was observed. In both cases of muscles, sarcomere length …

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Effect Of Freezing Rate On Goat Sperm Morphology And Dna Integrity, Burcu Üstüner, Zekari̇ya Nur, Seli̇m Alçay, Mehmed Berk Toker, Hakan Sağirkaya, Mustafa Kemal Soylu Jan 2015

Effect Of Freezing Rate On Goat Sperm Morphology And Dna Integrity, Burcu Üstüner, Zekari̇ya Nur, Seli̇m Alçay, Mehmed Berk Toker, Hakan Sağirkaya, Mustafa Kemal Soylu

Turkish Journal of Veterinary & Animal Sciences

This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 °C to -150 °C (G10: 10 °C/min, G12: 12 °C/min, G15: 15 °C/min, and G24: 24 °C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected …

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Melatonin Receptor 1a Gene Rsai And Inhibin Alpha Subunit Gene Haeii Polymorphisms In Honamli And Hair Goat Breeds Reared In Western Mediterranean Region Of Turkey, Özgecan Korkmaz Ağaoğlu, Mustafa Saatci, Bi̇lal Akyüz, Özkan Elmaz, Mehmet Çolak, Burcu Menekşe Balkan, Emel Zeytünlü Jan 2015

Melatonin Receptor 1a Gene Rsai And Inhibin Alpha Subunit Gene Haeii Polymorphisms In Honamli And Hair Goat Breeds Reared In Western Mediterranean Region Of Turkey, Özgecan Korkmaz Ağaoğlu, Mustafa Saatci, Bi̇lal Akyüz, Özkan Elmaz, Mehmet Çolak, Burcu Menekşe Balkan, Emel Zeytünlü

Turkish Journal of Veterinary & Animal Sciences

The melatonin receptor 1A (MTNR1A) and inhibin alpha subunit (INHA) genes play a significant role in the reproductive characteristics of animals. Blood samples were collected from 371 goats (Honamli and Hair) reared in Antalya and Burdur. The polymerase chain reaction (PCR) products were digested by RsaI for the MTNR1A gene and HaeII for the INHA gene. Two alleles (A and G) and three genotypes (AA, AG, and GG) were observed for the INHA gene, while two alleles (R and r) and two genotypes (RR and Rr) were observed for the MTNR1A gene. The highest allelic frequency value for G (91.8%) …

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Determination Of The Alui Polymorphism Effect Of Bovine Growth Hormone Gene On Carcass Traits In Zavot Cattle With Analysis Of Covariance, Aytaç Akçay, Bi̇lal Akyüz, Davut Bayram Jan 2015

Determination Of The Alui Polymorphism Effect Of Bovine Growth Hormone Gene On Carcass Traits In Zavot Cattle With Analysis Of Covariance, Aytaç Akçay, Bi̇lal Akyüz, Davut Bayram

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the genotypes of bovine growth hormone and to investigate their association with daily weight gain in the Zavot cattle breed. The bovine growth hormone (GH) genotypes of 45 male Zavot cattle were identified by PCR-RFLP. The effect of the bovine growth hormone gene on daily weight gain was estimated from a dataset obtained from cattle up to the age of 9 months. The mean differences of carcass weight or carcass yield among the genotype groups were assessed by analysis of covariance (ANCOVA). Two alleles for bovine growth hormone locus, L and V, …

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Effects Of Dystocia On Milk Production And Reproduction In Subsequent Lactation In A Turkish Holstein Herd, İbrahi̇m Kaya, Can Uzmay, Tarik Ayyilmaz Jan 2015

Effects Of Dystocia On Milk Production And Reproduction In Subsequent Lactation In A Turkish Holstein Herd, İbrahi̇m Kaya, Can Uzmay, Tarik Ayyilmaz

Turkish Journal of Veterinary & Animal Sciences

The study investigated effects of dystocia on milk production, reproduction, calf loss, and cow culling in a Turkish Holstein herd at the Agricultural Faculty, Ege University, İzmir, Turkey. Data were from 653 single calvings between 2006 and 2010. Considering a greater dystocia incidence in primiparous cows, data was divided in two; 223 primiparous and 430 multiparous calvings scored on a 1-to-5 scale. Scores were categorized into no dystocia and dystocia. With few related observations, all multiparous data were excluded since sample size for multiparous dystocic calvings was too small to make reliable estimates. Milk yields, days to first service, and …

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Functional Properties Of Gelatin Recovered From Scales Of Farmed Sea Bass (Dicentrarchus Labrax), Mehmet Tolga Di̇nçer, Özlem Yeşi̇m Ağçay, Hülya Sargin, Huri̇ye Bayram Jan 2015

Functional Properties Of Gelatin Recovered From Scales Of Farmed Sea Bass (Dicentrarchus Labrax), Mehmet Tolga Di̇nçer, Özlem Yeşi̇m Ağçay, Hülya Sargin, Huri̇ye Bayram

Turkish Journal of Veterinary & Animal Sciences

In the current study gelatin was recovered from scales of farmed sea bass (Dicentrarchus labrax). The first part of the study was planned to determine the yield of acid-soluble collagen and pepsin-soluble collagen from the scales. Both collagens were characterized as type I collagen, containing \alpha-1 and \alpha-2 chains. In the second part, gelatin was extracted using acetic acid and functional properties were determined in this product. Gelatin protein value was found to be 96% and the yield was found to be 18.49%. Proline and hydroxyproline values in the amino acid were found to be high. The gel strength of …

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Heart Performance Of Lambs And Its Relation To Muscle Volume And Body Surface, Róbert Romvári, Csaba Hancz, Péter Horn, Zsolt Petrási, András Szabó, Dávid Mezöszentgyörgyi, Imre Repa Jan 2015

Heart Performance Of Lambs And Its Relation To Muscle Volume And Body Surface, Róbert Romvári, Csaba Hancz, Péter Horn, Zsolt Petrási, András Szabó, Dávid Mezöszentgyörgyi, Imre Repa

Turkish Journal of Veterinary & Animal Sciences

Electrocardiography-gated dynamic magnetic resonance imaging (MRI) methodology was developed for the in vivo examination of sheep heart characteristics. By combining noninvasive determination of skeletal muscle mass, we studied the relationship between total body skeletal muscle content and heart performance. Measurements were carried out on male Merino lambs using 1.5 T field strength equipment. Average left ventricular volumes were determined and stroke volume was estimated during image postprocessing. Ejection fraction was calculated (73 ± 1.8%) and the cardiac output (CO) value was estimated (2.75 ± 0.16 L/min). After measuring left ventricular wall thickness, contraction values were determined at the septum (62%), …

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Cell-Based Therapy And Rehabilitation With Prosthetic Limbs In A Dog, Jayakrushna Das, Indramani Nath, Padmanav Routray, Rabindra Kumar Das, Sidhartha Sankar Behera Jan 2015

Cell-Based Therapy And Rehabilitation With Prosthetic Limbs In A Dog, Jayakrushna Das, Indramani Nath, Padmanav Routray, Rabindra Kumar Das, Sidhartha Sankar Behera

Turkish Journal of Veterinary & Animal Sciences

A 5-year-old male dog was presented with the complaint of a chronic nonhealing wound on both hind legs. The wound occurred during a serious train accident that caused loss of both hind limbs below the hock joint. Since then, the wound did not respond to any standard treatment for up to 4 months. Again it was dressed for 15 days, but it did not show any improvement. With the consent of the owner, cell-based therapy was conducted by collecting bone marrow from the proximal anteromedial aspect of the tibia. After the application of bone marrow derived mesenchymal stem cells (BM-MSCs) …

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In Vivo Assessment Of Dna Damage In Cyprinus Carpio After Exposure To Potassium Dichromate Using Rapd, Pavan Kumar, Ravindra Kumar, Naresh Sahebrao Nagpure, Prakash Nautiyal, Basdeo Kushwaha, Christopher Didigwu Nwani, Wazir Singh Lakra Jan 2015

In Vivo Assessment Of Dna Damage In Cyprinus Carpio After Exposure To Potassium Dichromate Using Rapd, Pavan Kumar, Ravindra Kumar, Naresh Sahebrao Nagpure, Prakash Nautiyal, Basdeo Kushwaha, Christopher Didigwu Nwani, Wazir Singh Lakra

Turkish Journal of Veterinary & Animal Sciences

The present study used the random amplification of polymorphism DNA (RAPD) technique to evaluate possible DNA damage in the blood cells of Cyprinus carpio exposed to potassium dichromate. Test specimens were exposed to 3 sublethal test concentrations (93.95, 187.9, and 281.85 mg L^{?1}) of potassium dichromate for a period of 96 h. The samplings were collected at 24, 48, 72, and 96 h after exposure. DNA was isolated from the blood samples of both the control and treated groups of C. carpio, and 3 selected decamer primers were used for RAPD-PCR. In comparison to the control, potassium dichromate-treated groups showed …

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Molecular Characterization Of The Hspa1a Gene By Single-Strandconformation Polymorphism And Sequence Analysis In Holstein-Friesiancrossbred And Deoni Cattle Raised In India, Ramesha P. Kerekoppa, Akhila Rao, Marappa Basavaraju, Gujjar Ramachandra Geetha, Lakhsminarayan Krishnamurthy, Thota Vl Narasimha Rao, Dayal Nitai Das, Kataktalware Mukund Jan 2015

Molecular Characterization Of The Hspa1a Gene By Single-Strandconformation Polymorphism And Sequence Analysis In Holstein-Friesiancrossbred And Deoni Cattle Raised In India, Ramesha P. Kerekoppa, Akhila Rao, Marappa Basavaraju, Gujjar Ramachandra Geetha, Lakhsminarayan Krishnamurthy, Thota Vl Narasimha Rao, Dayal Nitai Das, Kataktalware Mukund

Turkish Journal of Veterinary & Animal Sciences

A group of proteins called heat shock proteins (HSPs) are synthesized during heat stress. They are expressed at high levels when exposed to stress. Polymorphism in HSPs has been reported to be associated with heat tolerance and reproductive performance in cattle. The present study aimed to investigate the polymorphisms in the coding region of the HSPA1A gene in Holstein-Friesian (HF) crossbreds and the Deoni breed of cattle. Genomic DNA was extracted from 94 animal blood samples and was subjected to polymerase chain reaction-single strand conformation polymorphism analysis, which revealed 14 band patterns in Deoni cattle and 8 band patterns in …

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Genetic Diversity Of Village Chickens In Central Black Sea Region And Commercial Chickens In Turkey By Using Microsatellite Markers, Levent Mercan, Ahmet Okumuş Jan 2015

Genetic Diversity Of Village Chickens In Central Black Sea Region And Commercial Chickens In Turkey By Using Microsatellite Markers, Levent Mercan, Ahmet Okumuş

Turkish Journal of Veterinary & Animal Sciences

Local chicken populations in the Central Black Sea Region of Turkey have been intensively affected by governmental poultry culling due to avian influenza outbreak risks. The aim of this study was to investigate the genetic diversity of indigenous chicken populations raised in the Central Black Sea Region in order to assess genetic structures of these populations and to determine genetic relationships between the study populations and certain commercial chicken genotypes. Genotypic diversity of 45 Turkish village chicken populations located in 5 provinces in the Central Black Sea Region of Turkey and 2 commercial hybrid populations were compared using 28 autosomal …

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The Effect Of Oleic And Linoleic Acids On In Vitro Bovineembryonic Development And Embryo Quality, Tahi̇r Karaşahi̇n, Şevket Arikan Jan 2015

The Effect Of Oleic And Linoleic Acids On In Vitro Bovineembryonic Development And Embryo Quality, Tahi̇r Karaşahi̇n, Şevket Arikan

Turkish Journal of Veterinary & Animal Sciences

The present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid (10, 100, and 1000 µM). Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates (P < 0.01). It was determined that the highest dose of linoleic acid (1000 µM) and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morula-blastocyst stage (P < 0.001). As a result, while oleic acid significantly increased (P < 0.001) the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.

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Immunohistochemical Distribution Of Platelet-Derived Growth Factor-C And Platelet-Derived Growth Factor Receptor-? In Small Intestine Of Rats Treated With Capsaicin, Buket Bakir, Ebru Karadağ Sari Jan 2015

Immunohistochemical Distribution Of Platelet-Derived Growth Factor-C And Platelet-Derived Growth Factor Receptor-? In Small Intestine Of Rats Treated With Capsaicin, Buket Bakir, Ebru Karadağ Sari

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to examine the effect of capsaicin administration immunohistochemically on platelet-derived growth factor (PDGF)-C and platelet-derived growth factor receptor (PDGFR)-\alpha release in the small intestine tissues of rats. Rats were divided into three groups: group 1 (subcutaneous drug administration), group 2 (oral drug administration), and group 3 (subcutaneous sham administration). Histometric measurements and counts were performed in order to determine whether there was a difference between groups in terms of height of villi, depth of crypts, and goblet cell count in small intestine. The small intestine was examined by Crossman?s triple staining method and streptavidin-biotin …

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Influences Of Flunixin And Tenoxicam On The Pharmacokinetics Of Florfenicol In Lipopolysaccharide-Induced Endotoxemia, Feri̇de Koç, Alptuğ Ati̇la, Emre Karakuş, Kami̇l Üney Jan 2015

Influences Of Flunixin And Tenoxicam On The Pharmacokinetics Of Florfenicol In Lipopolysaccharide-Induced Endotoxemia, Feri̇de Koç, Alptuğ Ati̇la, Emre Karakuş, Kami̇l Üney

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to investigate the influences of flunixin (FM) and tenoxicam (TN) on the pharmacokinetics of florfenicol (FF) after coadministration in lipopolysaccharide (LPS)-induced endotoxemic rabbits. Fifteen male rabbits were used in this study. FF (20 mg/kg), FM (2 mg/kg), and TN (1 mg/kg) were coadministered via intravenous injection to the animals. The concentrations of FF were determined by high-performance liquid chromatography with diode-array detection from 0.08 to 12 h in plasma. The plasma concentration-time profile of FF was described using a noncompartmental open model. In this study, terminal half-life, area under the curve, mean residence time, …

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The Effects Of Hypoxia On The Expression Of Vascular Endothelialgrowth Factor In Broiler Lung Fibroblasts, Musa Özgür Özyi̇ği̇t, Müjdat Müfi̇t Kahraman, Ahmet Akkoç Jan 2015

The Effects Of Hypoxia On The Expression Of Vascular Endothelialgrowth Factor In Broiler Lung Fibroblasts, Musa Özgür Özyi̇ği̇t, Müjdat Müfi̇t Kahraman, Ahmet Akkoç

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effects of hypoxic stresses on vascular endothelial growth factor (VEGF) in cultured lung fibroblasts of meat-type chickens. Isolated and subcultured primary lung fibroblasts were exposed to hypoxia. The presence of VEGF and the effects of hypoxia on cultured lung fibroblasts and in the culture media were evaluated. Immunocytochemistry using streptavidin-biotin-peroxidase method and ELISA using commercially available primary monoclonal antibodies were used for evaluation in both 6-h and 12-h hypoxic fibroblasts. The level of VEGF was slightly higher in the 6-h hypoxic group (1.22 ng/mL) than in the 6-h control group (1.27 …

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