Veterinary Medicine Commons^™

Temperatures Of Storage Areas In Large Animal Veterinary Practice Vehicles In The Summer And Comparison With Drug Manufacturers’ Storage Recommendations, Jeff D. Ondrak, Meredyth L. Jones, Virginia R. Fajt

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Large animal veterinarians carry drugs in their practice vehicles in storage areas that are not typically refrigerated. The most common upper limits of manufacturers’ storage temperatures for United States (U.S.)-approved non-refrigerated drugs are 25 or 30 °C. Because ambient temperatures in many locations in the U.S. exceed these temperatures during the summer, we measured storage area temperatures over 4 months in the summer of 2013 to evaluate the extent to which labeled storage temperatures are exceeded.

Methods: A convenience sample of 12 vehicles from 5 central Texas practices and 12 vehicles from 4 south central Nebraska practices was …

Nonlinear Hierarchical Models For Longitudinal Experimental Infection Studies, Michael David Singleton

Theses and Dissertations--Epidemiology and Biostatistics

Experimental infection (EI) studies, involving the intentional inoculation of animal or human subjects with an infectious agent under controlled conditions, have a long history in infectious disease research. Longitudinal infection response data often arise in EI studies designed to demonstrate vaccine efficacy, explore disease etiology, pathogenesis and transmission, or understand the host immune response to infection. Viral loads, antibody titers, symptom scores and body temperature are a few of the outcome variables commonly studied. Longitudinal EI data are inherently nonlinear, often with single-peaked response trajectories with a common pre- and post-infection baseline. Such data are frequently analyzed with statistical methods …

Reversible Downregulation Of Hypothalamic-Pituitary-Gonadal Axis In The Stallion With A Third-Generation Gnrh Antagonist, Gabriel Monteiro Davolli

Theses and Dissertations--Veterinary Science

The objectives of this thesis were: (1) to evaluate the downregulation of the stallion hypothalamic-pituitary-gonadal (HPG) axis by a GnRH antagonist (acyline) based upon endocrine, seminal, testicular and behavioral effects, and (2) to assess recovery after treatment. Stallions were treated for 50 days (n=4; 330µg/kg acyline q 5d) and controls (n=4) received vehicle alone. Stallions were assessed pre-treatment and for 72 days after last treatment. Treatment induced declines (p<0.05) in FSH, LH, testosterone (to castrate levels) and estrone sulfate. Gonadotropins and testosterone returned to control values within nine days and estrone sulfate by 14 days after treatment discontinuation. Acyline-treated stallions failed to respond with FSH, LH and testosterone increase after exogenous GnRH stimulation (25µg gonadorelin, IV) compared to pre-treatment and control stimulation. Total sperm numbers and motility were reduced in acyline-treated stallions, as well as total seminal plasma protein and testicular volume (p<0.05). Time to ejaculation was increased in acyline group (p<0.5). Testicular, sexual behavior and most seminal parameters regained normal levels within 72 days after treatment ceased. Sperm output of acyline-treated stallions was regained within seven months after ending treatment. Acyline reversibly suppressed the stallion HPG axis, thus has potential for treating the androgen-dependent Equine-Arteritis-Virus carrier state and as behavior modulator.

Glucocorticoid-Induced Chondrocyte Cytotoxicity At Doses Recommended For Intra-Articular Therapy In Horses, Wenying Zhu

Theses and Dissertations--Veterinary Science

Intra-articular glucocorticoid injections are commonly used to treat synovitis and osteoarthritis in horses. These agents are highly effective at relieving pain, swelling, and other symptoms of joint inflammation. The drugs also have therapeutic benefits by down regulating the expression of cytokines and protease enzymes that participate in the degradation of articular cartilage. However, detrimental effects on chondrocyte function and cell viability that is independent of osteoarthritis pathogenesis have been described and linked to glucocorticoid use. These side effects are both drug- and dose-dependent. This study tested the hypothesis that manufacture recommended dosage levels of methylprednisolone, betamethasone, and triamcinolone that are …

Uterine Artery Rupture, An Angiopathy Of The Reproductive System Of The Mare: Occurrence And Potential Effects, Ana G. Toro Mayorga

Theses and Dissertations--Veterinary Science

The intent of this research was to identify if the degenerative changes within arteries in the endometrium (endometrial angiopathies) correlate with degenerative changes in the uterine arteries and can be used as a predictor of increased risk for uterine artery rupture (UAR). With this objective specimens from 20 mares that died from uterine artery rupture and 21 control mares that died from unrelated causes were obtained from cases submitted to the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) over a two-year period. Postmortem specimens of each mare were collected from the left and right uterine arteries at the origin, bifurcation, …

Toxicity Of Leaf Extract Of Acer Spp. On Equine Erythrocytes, Bailey Katherine Dye

Undergraduate Honors Thesis Projects

Red maple toxicosis is a phenomenon that occurs in equines after the consumption of dried or wilted red maple (Acer rubrum L.) leaves. Within the erythrocytes, it causes hemolysis, methemoglobinemia, and Heinz body development. The toxic agent of red maple leaves has not yet been identified; however, the development of hemolytic anemia and methemoglobinemia in equines after ingestion of the leaves, suggests that the toxin is acting as a strong oxidant. Some studies have noted that not only are wilted red maples leaves toxic to equines, but that wilted leaves of other maple species may also be toxic. Therefore, …

The Role Of Herpes Simplex Virus Type 1 Glycoprotein K In Neuroinvasion And Immunopathogenesis Of Herpes Keratitis, Ahmad Saied

LSU Doctoral Dissertations

ABSTRACT Herpes simplex virus type 1 is a common neurotropic pathogen responsible for a multitude of human diseases ranging from mucocutaneous lesions, keratitis to life-threatening encephalitis. The hallmark of the HSV-1 life cycle is infection of sensory neurons, where the virus establishes a latent infection for the life of the host. Viral envelope glycoproteins play important roles in viral life cycle and virus-host interaction. Viral glycoproteins gK, gM, gE and the membrane protein UL11 have been shown to be important for virus assembly, egress and virus spread. To investigate the relative importance of each of gK, gM, gE and UL11 …

Comparative Genomic Studies Of Catfish And Zebrafish Strains Of Edwardsiella Ictaluri, Rui Wang

LSU Doctoral Dissertations

Edwardsiella ictaluri is a gram negative bacterium that is the causative agent of enteric septicemia of catfish. In 2011, this bacterium was identified as the causative agent of massive death in zebrafish populations in U.S. In this project, we found that isolates of E. ictaluri from zebrafish comprise a unique strain that differs from the type strain of E. ictaluri phenotypically as well as genetically. Also, strains of E. ictaluri from zebrafish are non-infectious in channel catfish Ictalurus punctatus by immersion. Here we sequenced the zebrafish strains of E. ictaluri LADL11-100 and LADL11-194 and compared the potential virulence genes in …

The Effects Of Prostatic Fluid On Functional Characteristics Of Cooled Canine Semen, Reto Fritsche

LSU Master's Theses

The objectives of this study were to investigate concentration dependent effects of canine prostatic fluid (PF) on in vitro seminal parameters of cooled canine semen. Sperm motility parameters, plasma membrane integrity and stability, acrosome integrity and DNA fragmentation were measured after the addition of 0%, 10%, 25%, or 50% PF to extended semen of fertile dogs. Assessments were made at 0 h pre-cooling, at 24, and 48 h of cooled storage (4 °C), and after freezing and thawing followed by incubation (37 °C) at 0, 4 and 24 h. Our hypothesis was that lower dilutions of canine semen with PF …

Supplementing Cow-Calf Pairs Grazing Smooth Bromegrass, Jason M. Warner, Annie J. Doerr, Galen E. Erickson Erickson, Rick J. Rasby, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A three-year study evaluated supplementing ethanol co-products mixed with low-quality forage to cow-calf pairs grazing smooth bromegrass as a method to replace grazed forage intake. Supplementing a 30:70 modified distillers grains plus solubles:cornstalks mixture reduced estimated grazed forage intake by approximately 40%. Doubling the stocking rate and supplementing did not impact cow or calf performance. A summer supplementation program designed to reduce grazed forage intake is a viable strategy for increasing stocking rate if forage for grazing is limited.

Effect Of Post-Weaning Management And Age At Weaning On Calf Growing And Finishing Performance, Jason M. Warner, Curtis J. Bittner, Karla H. Jenkins Jenkins, Rick J. Rasby, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

The impact of post-weaning management system and calf age at weaning on growing and finishing performance was evaluated. During the growing phase, cattle in the fast-track system had improved intake, gain, and feed conversion. Although initial finishing weight was similar between systems, slow-track cattle had greater intake, gain, final body weight, and carcass weight. While the impact of age at weaning was negligible, the improvement in finishing performance for slow-track cattle demonstrates the value of different management systems.

Estimation Of British- And Continental-Specific Heterosis Effects For Birth, Weaning, And Yearling Weight In Cattle, Lauren N. Schiermiester, R. Mark Thallman, Larry A. Kuehn Kuehn, Matthew L. Spangler

Nebraska Beef Cattle Reports

Heterosis, assumed proportional to expected breed heterozygosity, was calculated for 6,834 individuals with birth, weaning, and yearling weight records from Cycle VII of the U.S. Meat Animal Research Center Germplasm Evaluation Program. Heterosis was further estimated by proportions of British x British (BxB), British x Continental (BxC), and Continental x Continental (CxC) crosses. Estimates of BxB, BxC, and CxC heterosis were significant for weaning and yearling weight. This study illustrated that differences among biological types exist and provide an opportunity to utilize specific breeds and exploit heterosis in a crossbreeding system to achieve production goals.

The Effects Of Source And Amount Of Nitrite On Quality Characteristics Of All-Beef Frankfurters, Eric Miller, Chad G. Bower, Amy L. Redfield Redfield, Gary A. Sullivan

Nebraska Beef Cattle Reports

In an effort to meet consumers’ demand for foods with more natural ingredients, processors have begun manufacturing meat products cured with natural nitrite sources. The objective of this study was to evaluate the quality characteristics of all-beef frankfurters cured with traditional or alternative sources of nitrite and using equivalent amounts of nitrite. Frankfurters cured with alternative sources of nitrite had a slightly darker, less red exterior and slightly more yellow interior than those containing sodium nitrite. No differences were observed for pH or water activity. Both curing methods can be used to manufacture all-beef frankfurters with similar characteristics when using …

Stocking Rate Effects On Forage Nutrient Composition In Early Summer Pastures, Jared V. Judy, Jacki A. Musgrave, L. Aaron Stalker Stalker, K. H. Jenkins, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Nebraska Sandhills upland range pastures were used to measure the effectsof stocking rate on forage nutrient content in early summer pastures. Stocked pastures had lower CP, in vitroorganic matter digestibility, forage availability, and higher NDF compared with ungrazed pastures. Clipped samples of current year growth had greater CP and in vitro organic matter digestibility than diet samples. Observed results indicate early season grazing decreasesdiet nutrient content and forage availability compared with ungrazed pastures, suggesting that cattle were consuming both current and previous year growth.

Comparison Of Commercial Lick Tubs To Distillers Grains Supplementation For Calves Grazing Corn Residue, Mandi Jones, James C. Macdonald, Galen E. Erickson Erickson, Terry J. Klopfenstein, Kathleen R. Brooks, Dirk B. Burken, Robby Bondurant, Andrea K. Watson

Nebraska Beef Cattle Reports

Steer calves grazing irrigated corn residue were supplemented dried distillers grains plus solubles (DGS) or allowedcontinuous access to a commercial lick tub. Dried DGS was fed at 2.94 lb/steer/day and the lick tubs were consumed at 2.04 lb/steer/day (DM basis). Gain was greater for cattle supplemented with dried DGS (1.36 lb/day) compared to those with access to lick tubs (0.83 lb/day). Supplement efficiency varied between calves receiving dried DGS (46%) and those with continuous access to the lick tub (43%) when expressed on a DM basis. Values for dried DGS supplementation (48%) were not different for supplement efficiencyon an OM …

Nutrient Digestibility And Ruminal Ph Of Finishing Diets Containing Dry Milling Byproducts With And Without Oil Extraction, Melissa L. Jolly-Breithaupt, Adam L. Shreck, Jana L. Harding Harding, James C. Macdonald, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

A metabolism trial was conducted to determine the effects of corn oil removal in condensed distillers solubles (CDS) and modified distillers grains plus solubles (MDGS) on nutrient digestibility and ruminal pH. Oil removal had no impact on DM, OM, or NDF digestibility in steers fed CDS or MDGS. However, steers fed de-oiled CDS had a lower fat digestibility than steers fed normal CDS. Average ruminal pH was lower for steers fed de-oiled MDGS than for steers fed normal MDGS, however no difference within CDS was observed.

Effect Of Distillers Grains Plus Solubles And Monensin Supplementation On Grazing Steers, Tyler L. Hasenauer, Terry J. Klopfenstein, James C. Macdonald Donald, Robby G. Bondurant, Dirk B. Burken

Nebraska Beef Cattle Reports

Yearling steers rotationally grazing smooth bromegrass were individually supplemented monensin at 0 or 200 mg with modified distillers grains plus solubles (MDGS) at .05, 0.4, 0.6, and 0.8% BW. Cannulated steers continuously grazing smooth bromegrass were assigned randomly to one of two treatments: 0.4% BW MDGS supplementation with 0 or 200 mg monensin. Monensin did not affect ADG of steers supplemented MDGS ≥ 0.4% BW. Steers supplemented with monensin had a decreasein estimated average forage intakefrom 16.16 lb to 14.75 lb/OM daily.

Effects Of Processing Treated Corn Stover And Distillers Grains On Performance Of Growing Cattle, Jana L. Harding, Curtis J. Bittner, Dirk B. Burken Burken, Galen E. Erickson, James C. Macdonald

Nebraska Beef Cattle Reports

A study evaluated the effects of replacinga diet consisting of 60% corn stover, 18% solubles, and 18% distillers grains with a complete pelleted feed containing calcium oxide (CaO) treated corn stover and distillers grains on growing cattle performance. The pelleted feed was either pair-fed to the control treatment or fed ad libitum. There were no differences in ending BW, ADG, or F:G between the control and pair-fed treatment. Feeding the pellet ad libitum resulted in greater DMI and ADG; however, the cattle had greater F:G. The pellet has 98% the feeding value of the control treatment.

Effects Of Replacing Corn With A Pelleted Treated Corn Stover And Distillers Grains On Intake And Total Tract Digestibility Of Finishing Diets, Jana L. Harding, Melissa L. Jolly-Breithaupt, Galen E. Erickson Erickson, James C. Macdonald

Nebraska Beef Cattle Reports

A digestion study was conducted to evaluate the effects of replacing dry-rolled corn (DRC) with a pelleted feed containing treated corn stover, solubles, and distillers grains (DDG). Replacing DRC with the pelleted feed had no effect on intakes. Similarly, total tract digestibilities of DM, OM, or NDF were not affected by dietary treatment. There was a tendency for differences in average ruminalpH between treatments; however, proportions of acetate, propionate, and butyrate were not impacted. It was concluded that the DRC could be replacedwith a pelleted stover and distillers in the finishing diet without altering total tract digestion.

Effect Of 300 Or 400 Mg Daily Of Ractopamine Hydrochloride On Growth Performance And Carcass Characteristics Of Finishing Steers During The Last 14, 28, Or 42 Days, Curtis J. Bittner, Dirk B. Burken, Adam L. Shreck Shreck, James C. Macdonald, Galen E. Erickson, Nathan A. Pyatt

Nebraska Beef Cattle Reports

The effects of ractopamine hydrochloride (Optaflexx®) dosage (0, 300, and 400 mg/head/day) and duration (14, 28, or 42 days) on growth performance were evaluated in feedlot finishing diets. Feeding 300 mg of Optaflexx for 28 or 42 days increased live final BW by 13 and 29 lb, while feeding Optaflexx at 400 mg resulted in 27 or 24 lb increases relative to 0 mg steers, respectively. Feeding 300 mg of Optaflexx for 28 or 42 days would suggest 11.1 or 16.6 lb improvements in HCW, while feeding 400 mg of Optaflexx would suggest 19.7 or 20.7 lb heavier carcasses compared …

Genetic Parameter Estimates For Calving Difficulty And Birth Weight In A Multibreed Population, Cashley M. Ahlberg, Larry A. Kuehn, R. Mark Thallman Thallman, Stephen D. Kachman, Matthew L. Spangler

Nebraska Beef Cattle Reports

Eighteen breeds were utilized to estimate genetic parameters for birth weight and calving difficulty on first-parity females. Birth weight and calving difficulty were moderately heritable allowing for genetic selection to decrease calving difficulty. Genetic correlation estimates were positive between direct effects for birth weight and calving difficulty. This work will serve as the foundation for estimating across-breed EPD for calving difficulty in the U.S.

Show Horse Welfare, Melissa A. Voigt

Open Access Dissertations

In recent years there has been an increase in the public's attention to situations where trainers, owners, and handlers have compromised the well-being of show horses for the sake of winning. These situations may be due to training negligence or naivety of individuals working with the horse. Either way, due to these incidents, increasing pressure has been placed on the horse industry to address show horse welfare. The purpose of this research was to expound on the welfare of stock-type show horses through the perspective of those directly involved; considering the understanding of welfare, the value placed on welfare, and …

Taxonomy And Molecular Epidemiology Of Echinococcus Granulosus Sensu Lato, Thomas Romig, Dennis Ebi, Marion Wassermann

Harold W. Manter Laboratory of Parasitology: Library Materials

Echinococcus granulosus, formerly regarded as a single species with a high genotypic and phenotypic diversity, is now recognized as an assemblage of cryptic species, which differ considerably in morphology, development, host specificity (including infectivity/pathogenicity for humans) and other aspects. This diversity is reflected in the mitochondrial and nuclear genomes and has led to the construction of phylogenetic trees and hypotheses on the origin and geographic dispersal of various taxa. Based on phenotypic characters and gene sequences, E. granulosus (sensu lato) has by now been subdivided into E. granulosus sensu stricto (including the formerly identified genotypic variants G1-3), …

Mutations In The 5’ Ntr And The Non-Structural Protein 3a Of The Coxsackievirus B3 Selectively Attenuate Myocarditogenicity, Chandirasegaran Massilamany, Arunakumar Gangaplara, Rakesh H. Basavalingappa, Rajkumar A. Rajasekaran, Hiep Vu, Jean-Jack Riethoven, David Steffen, Asit K. Pattnaik, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

The 5’ non-translated region (NTR) is an important molecular determinant that controls replication and virulence of coxsackievirus B (CVB)3. Previous studies have reported many nucleotide (nt) sequence differences in the Nancy strain of the virus, including changes in the 5’ NTR with varying degrees of disease severity. In our studies of CVB3-induced myocarditis, we sought to generate an infectious clone of the virus for routine in vivo experimentation. By determining the viral nt sequence, we identified three new nt substitutions in the clone that differed from the parental virus strain: C97U in the 5’ NTR; a silent mutation, A4327G, in …

Confocal Fluorescence Microscopy: An Ultra-Sensitive Tool Used To Evaluate Intracellular Antiretroviral Nano-Drug Delivery In Hela Cells, Subhra Mandal, You Zhou, Annemarie Shibata, Christopher J. Destache

School of Veterinary and Biomedical Sciences: Faculty Publications

In the last decade, confocal fluorescence microscopy has emerged as an ultra-sensitive tool for real-time study of nanoparticles (NPs) fate at the cellular-level. According to WHO 2007 report, Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) is still one of the world’s major health threats by claiming approximately 7,000 new infections daily worldwide. Although combination antiretroviral drugs (cARV) therapy has improved the life-expectancy of HIV-infected patients, routine use of high doses of cARVhas serious health consequences and requires complete adherence to the regimen for success. Thus, our research goal is to fabricate long-acting novel cARV loaded poly(lactide-co-glycolic acid) (PLGA) nanoparticles (cARV-NPs) as …

Association Of Autophagy In The Cell Death Mediated By Dihydrotestosterone In Autoreactive T Cells Independent Of Antigenic Stimulation, Ting Jia, Annandurai Anandhan, Chandirasegaran Massilamany, Rajkumar A. Rajasekaran, Rodrigo Franco, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Gender disparity is well documented in the mouse model of experimental autoimmune encephalomyelitis (EAE) induced with proteolipid protein (PLP) 139–151, in which female, but not male, SJL mice show a chronic relapsing-remitting paralysis. Furthermore, dihydrotestosterone (DHT) has been shown to ameliorate the severity of EAE, but the underlying mechanisms of its protective effects are unclear. Using major histocompatibility complex (MHC) class II dextramers for PLP 139-151, we tested the hypothesis that DHT selectively modulates the expansion and functionalities of antigen-specific T cells. Unexpectedly, we noted that DHT induced cell death in antigen-specific, autoreactive T cells, but the effects were not …

Evaluation Of An Interactive Workshop Designed To Teach Practical Welfare Techniques To Beef Cattle Caretakers And Decision Makers, Reneé Dewell, Christy Hanthorn, Jared Danielson, Rebecca Burzette, Johann Coetzee, D. Dee Griffin, Alejandro Ramirez, Grant Dewell

School of Veterinary and Biomedical Sciences: Faculty Publications

The purpose of the project was to evaluate the use of an interactive workshop designed to teach novel practical welfare techniques to beef cattle caretakers and decision makers. Following training, respondents reported being more likely to use or recommend use of local anesthesia for dehorning and castration and were more inclined to use meloxicam for pain management. Respondents also reported improvement in performing all skills taught in the workshop. These results suggest instructional techniques used to teach practical concepts/techniques relevant to beef cattle welfare led to improvements in both perceived competency and estimated likelihood that the material will be used. …

Spiral Ganglion Degeneration And Hearing Loss As A Consequence Of Satellite Cell Death In Saposin B-Deficient Mice, Omar Akil, Ying Sun, Sarath Vijayakumar, Wujuan Zhang, Tiffany Ku, Chi-Kyou Lee, Sherri Jones, Gregory A. Grabowski, Lawrence R. Lustig

School of Veterinary and Biomedical Sciences: Faculty Publications

Saposin B (Sap B) is an essential activator protein for arylsulfatase A in the hydrolysis of sulfatide, a lipid component of myelin. To study Sap B’s role in hearing and balance, a Sap B-deficient (B^-/-) mouse was evaluated. At both light and electron microscopy (EM) levels, inclusion body accumulation was seen in satellite cells surrounding spiral ganglion (SG) neurons from postnatal month 1 onward, progressing into large vacuoles preceding satellite cell degeneration, and followed by SG degeneration. EM also revealed reduced or absent myelin sheaths in SG neurons from postnatal month 8 onwards. Hearing loss was initially seen …

Pseudorabies Virus Fast Axonal Transport Occurs By A Pus9- Independent Mechanism, Gina R. Daniel, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

Reactivation from latency results in transmission of neurotropic herpesviruses from the nervous system to body surfaces, referred to as anterograde axonal trafficking. The virus-encoded protein pUS9 promotes axonal dissemination by sorting virus particles into axons, but whether it is also an effector of fast axonal transport within axons is unknown. To determine the role of pUS9 in anterograde trafficking, we analyzed the axonal transport of pseudorabies virus in the presence and absence of pUS9.

Haemorrhagic Colitis Associated With Enterohaemorrhagic Escherichia Coli O165:H25 Infection In A Yearling Feedlot Heifer, Rodney A. Moxley, Zachary R. Stromberg, Gentry L. Lewis, John D. Loy, Bruce W. Brodersen, Isha R. Patel, Jayanthi Gangiredla

School of Veterinary and Biomedical Sciences: Faculty Publications

Introduction: Enterohaemorrhagic Escherichia coli (EHEC) cause haemorrhagic colitis and haemolytic uraemic syndrome in humans. Although EHEC infection typically results in haemorrhagic colitis in all ages of human patients, in cattle it is usually limited to 1- to 5-week-old nursing calves.

Case Presentation: A 1-year-old feedlot beef heifer was moribund with neurological signs and bloody diarrhoea. At necropsy, the colonic mucosa contained multiple grossly visible haemorrhagic erosions, each measuring <1 mm in diameter. Histologically, foci corresponding to the gross erosions had E. coli O165 antigen-positive bacterial rods adherent to the apical surfaces of degenerate and necrotic colonic mucosal epithelial cells in association with attaching and effacing lesions, and also within cytoplasmic vacuoles in …