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Articles 781 - 810 of 2510
Full-Text Articles in Veterinary Medicine
Use Of Essential Oils For Manipulation Of Rumen Microbial Fermentation Using Batch Culture, Atitthan Nanon, Wisitiporn Suksombat, Zhu Yang Wen
Use Of Essential Oils For Manipulation Of Rumen Microbial Fermentation Using Batch Culture, Atitthan Nanon, Wisitiporn Suksombat, Zhu Yang Wen
The Thai Journal of Veterinary Medicine
The objective of this study was to evaluate the effects of various levels of essential oils on feed digestibility, gas production and rumen fermentation. Two batch cultures were designed for screening various doses of each essential oil (EO). Treatments were control (CON), cinnamon oil (CIN), clove oil (CLO), garlic oil (GAR), ginger oil (GIN), and lemongrass oil (LEM). Dosages were 0, 200, 400, 800, and 1600 mg/kg DM in experiment I (1st batch) and 50, 100, 150, and 200 mg/kg DM in experiment II (2nd batch). Digestibility of DM (DMD), neutral detergent fiber and acid detergent fiber was measured at …
Infection Of Thai Influenza A Viruses Subtype H5n1 Using Tracheal Culture, Napawan Bunpapong, Supanat Boonyapisitsopa, Kamol Suwannakarn, Rachod Tantilertchareon, Roongroje Thanawongnuwech, Alongkorn Amonsin
Infection Of Thai Influenza A Viruses Subtype H5n1 Using Tracheal Culture, Napawan Bunpapong, Supanat Boonyapisitsopa, Kamol Suwannakarn, Rachod Tantilertchareon, Roongroje Thanawongnuwech, Alongkorn Amonsin
The Thai Journal of Veterinary Medicine
Avian influenza (AI) is caused by influenza A virus subtype H5N1. The virus causes severe disease and death in several avian and mammalian species. This study investigated the ability of swine and chicken tracheal cultures to support highly pathogenic avian influenza subtype H5N1 (HPAI-H5N1) viral replication. Three Thai HPAI-H5N1 viruses isolated from chicken (A/chicken/Thailand/CU-K2/04), duck (A/duck/Thailand/CU-328/07), and tiger (A/tiger/Thailand /CU-T7/04) were used to infect the swine and chicken tracheal cultures. Our results indicated that the chicken tracheal culture could support virus replication in contrast to the swine tracheal culture, suggesting that pig has low susceptibility to Thai HPAI-H5N1 infection. Immunohistochemical …
Double Dengue Serotypes In Asymptomatic Populations Living In An Area Of Thailand Endemic For Dengue Hemorrhagic Fever, Veerayuth Kittichai, Methee Sriprapun, Papawin Konklong, Nithita Thonsangin, Jakkrawarn Chompoosri, Apiwat Tawatsin, Usavadee Thavara, Padet Siriyasatien
Double Dengue Serotypes In Asymptomatic Populations Living In An Area Of Thailand Endemic For Dengue Hemorrhagic Fever, Veerayuth Kittichai, Methee Sriprapun, Papawin Konklong, Nithita Thonsangin, Jakkrawarn Chompoosri, Apiwat Tawatsin, Usavadee Thavara, Padet Siriyasatien
The Thai Journal of Veterinary Medicine
Dengue virus infection remains a major health problem worldwide. Understanding dengue infection and characterizing circulating viruses are essential for disease prevention and control as well as vaccine development. In this study, we aimed to identify dengue virus in healthy people living in an area endemic for dengue disease. Blood samples were collected from 52 healthy local subjects living in a dengue-endemic area of Thailand. Viral RNA was detected using a nested reverse transcription polymerase chain (RT-PCR) that amplified the E gene. Phylogenetic trees were constructed by the neighbor-joining method using MEGA6.06. Dengue virus was detected in 5 of 52 samples …
Ophthalmology Snapshot, Nalinee Tuntivanich
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Efficacy Of Salinomycin, Robenidine And Decoquinate Against Infection With Eimeria Species Field Isolate In A Densely Populated Broiler Farm In Thailand, Morakot Kaewthamasorn, Nataya Charoenvisal, Niwat Chansiripornchai
Efficacy Of Salinomycin, Robenidine And Decoquinate Against Infection With Eimeria Species Field Isolate In A Densely Populated Broiler Farm In Thailand, Morakot Kaewthamasorn, Nataya Charoenvisal, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
Avian coccidiosis is caused by intracellular protozoan parasites in the genus Eimeria. It has a significant economic impact on the poultry industry and is highly prevalent in chicken producing countries worldwide. Current control measurements including good animal husbandry, chemoprophylaxis, and vaccination cannot effectively control the disease. It is well understood that parasites repeatedly exposed to the same anticoccidial drug is a major cause of resistance. Therefore, it is critical to monitor parasite response to anticoccidial drugs in different field settings. The aim of this experiment was to investigate responses of a local field isolate of Eimeria species to commonly used …
Cryopreservation Effect On Expression Of Sex Steroid Receptors Of Boar Spermatozoa, Sukanya Manee-In, Panida Chanapiwat, Nawarus Prapaiwan, Kampon Kaeoket, Sayamon Srisuwatanasagul, Atthaporn Roongsitthichai
Cryopreservation Effect On Expression Of Sex Steroid Receptors Of Boar Spermatozoa, Sukanya Manee-In, Panida Chanapiwat, Nawarus Prapaiwan, Kampon Kaeoket, Sayamon Srisuwatanasagul, Atthaporn Roongsitthichai
The Thai Journal of Veterinary Medicine
This study aimed to investigate the expressions of estrogen receptor (ER) alpha, ER beta, and progesterone receptor (PR) during semen cryopreservation, to evaluate the correlation among ERa, ERβ, PR, capacitation, acrosome reaction, and semen quality parameters, and to investigate the effect of different concentrations of L-cysteine on the expressions of ERa, ERβ, and PR. Semen samples were collected from 12 boars [Duroc (n = 4), Landrace (n=4), and Large white (n=4)]. The controlled rate-freezing method was used to cryopreserve the semen samples. The samples were collected at different times: fresh semen, after adding ModenaTM, after adding extender II (TE2), after …
Antimicrobial Resistance Patterns And Flaa Genotypes Of Campylobacter Jejuni Isolated From Contracted Broiler Farms In Eastern Thailand, Petcharatt Charununtakorn, Sakaoporn Prachantasena, Taradon Luangtongkum
Antimicrobial Resistance Patterns And Flaa Genotypes Of Campylobacter Jejuni Isolated From Contracted Broiler Farms In Eastern Thailand, Petcharatt Charununtakorn, Sakaoporn Prachantasena, Taradon Luangtongkum
The Thai Journal of Veterinary Medicine
The increasing incidence of antibiotic-resistant Campylobacter has become a major public health concern. Since little is known about antimicrobial resistance of Campylobacter isolated from contracted broiler farms, the major type of farm that produces chicken meat for domestic consumption in Thailand, the objective of the present study was to determine the antimicrobial resistance patterns and genotypes of Campylobacter isolated from contracted broiler farms where antibiotics were routinely used in their production. Sixty-nine Campylobacter jejuni isolates from cloacal swabs of chickens reared in 2 small contracted broiler farms in eastern Thailand were tested for their susceptibility to 5 antimicrobial agents by …
Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram
Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram
The Thai Journal of Veterinary Medicine
No abstract provided.
Ultrasound Diagnosis, Phiwipha Kamonrat
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Effect Of Pcso-524 On Oa Biomarkers And Weight-Bearing Properties In Canine Shoulder And Coxofemeral Osteoarthritis, Kumpanart Soontornvipart, Natwadee Mongkhon, Korakot Nganvongpanit, Prachya Kongtawelert
Effect Of Pcso-524 On Oa Biomarkers And Weight-Bearing Properties In Canine Shoulder And Coxofemeral Osteoarthritis, Kumpanart Soontornvipart, Natwadee Mongkhon, Korakot Nganvongpanit, Prachya Kongtawelert
The Thai Journal of Veterinary Medicine
This study was designed to compare the therapeutic benefits of a compound of omega-3 fatty acids from the New Zealand green-lipped mussel (Perna canaliculus) (PCSO-524) and omega-3 fatty acids in fish oil on clinical outcomes and osteoarthritis biomarkers (chondroitin sulfate WF6 epitope) in 66 dogs that had osteoarthritis (OA); 39 dogs with OA hip joints, 15 dogs with OA shoulder joints and 12 dogs with OA shoulder and hip joints. The animals were presented at the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University. The dogs were allocated into two groups randomly. One group received PCSO-524 (n = 33) …
Effects Of Vitamin D3 And Calcium On Productive Performance, Egg Quality And Vitamin D3 Content In Egg Of Second Production Cycle Hens, Hatairat Plaimast, Suwanna Kijparkorn, Punyaphat Ittitanawong
Effects Of Vitamin D3 And Calcium On Productive Performance, Egg Quality And Vitamin D3 Content In Egg Of Second Production Cycle Hens, Hatairat Plaimast, Suwanna Kijparkorn, Punyaphat Ittitanawong
The Thai Journal of Veterinary Medicine
This experiment was conducted to investigate the effect of supplementary vitamin D3 on productive performance, internal egg quality, eggshell quality, apparent calcium digestibility and vitamin D3 content in egg of second production cycle hens fed on different levels of calcium. A total of 140 second cycle brown-egg laying hens, aged 98 weeks, were randomly divided into 5 treatments with 4 replicates. The treatments consisted of diets containing 3.5%Ca and 2,000 IU of vitamin D3/kg of feed (control), 3.5%Ca and 6,000 IU of vitamin D3/kg of feed (treatment 2), 3.75%Ca and 2,000 IU of vitamin D3/kg of feed (treatment 3), 3.75%Ca …
Analysis Of Virulence Genes And Pathogenicity Of Thai Mycoplasma Gallisepticum Isolates, Pacharaporn Khumpim, Rapeewan Tampaisarn, Jiroj Sasipreeyajan, Somsak Pakpinyo
Analysis Of Virulence Genes And Pathogenicity Of Thai Mycoplasma Gallisepticum Isolates, Pacharaporn Khumpim, Rapeewan Tampaisarn, Jiroj Sasipreeyajan, Somsak Pakpinyo
The Thai Journal of Veterinary Medicine
Mycoplasma gallisepticum (MG) causes chronic respiratory disease in poultry. MG organisms consist of several virulence genes which are related to infection. The aim of this study was to analyze the virulence genes and determine the pathogenicity of Thai MG isolates. In this study, there were 3 experiments; nineteen MG isolates; and 2 reference strains, F and S6 strains, analyzed to detect 4 virulence genes (LP, gapA, pvpA and mgc2) by PCR assay (experiment 1). The pathogenicity study of sham negative control group, S6 strain, 3 selected isolates (AHRL 31/46, 54/46 and 58/46) and F strain, respectively, were inoculated into 6 …
Genetic Correlations Among Average Daily Gain, Backfat Thickness And Sow Longevity In Landrace And Yorkshire Sows, Nuttha Wongsakajornkit, Nalinee Imboonta
Genetic Correlations Among Average Daily Gain, Backfat Thickness And Sow Longevity In Landrace And Yorkshire Sows, Nuttha Wongsakajornkit, Nalinee Imboonta
The Thai Journal of Veterinary Medicine
The present study aimed to estimate heritabilities as well as genetic correlations of sow longevity with average daily gain and backfat thickness. Removed sows included in the current study were 1,706 Landrace sows and 1,159 Yorkshire sows. They were raised in a Thai swine breeding herd and removed in the 2008-2013 period. Sow longevity was defined as the number of days from first farrowing date to removal date. Genetic parameters were estimated by using the Restricted Maximum Likelihood (REML) method with a multiple-trait animal model. On average, the sow longevity, average daily gain and backfat thickness were 633 days, 863 …
Effect Of Various Combinations Of Sugar Supplementation In The Extender On Frozen-Thawed Ram Semen Quality And Fertility, Saritvich Panyaboriban, Junpen Suwimonteerabutr, Nawapen Phutikanit, Theerawat Swangchan-Uthai, Theerawat Tharasanit, Mongkol Techakumphu
Effect Of Various Combinations Of Sugar Supplementation In The Extender On Frozen-Thawed Ram Semen Quality And Fertility, Saritvich Panyaboriban, Junpen Suwimonteerabutr, Nawapen Phutikanit, Theerawat Swangchan-Uthai, Theerawat Tharasanit, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Semen cryopreservation alters sperm structure and biochemical properties, and subsequently impairs fertilizing ability. A combination of two different sugar molecules revealed an enhanced beneficial effect on frozen-thawed spermatozoa of various species. However, the report in ram has been scarce. The present study investigated the effect of different combinations of sugar supplementation on post-thawed ram semen qualities in a cryopreservative extender in the presence of a single sugar or a combination of sugars. Data showed that a combination of sucrose and trehalose significantly improved cryopreserved sperm motility, viability, longevity and acrosome integrity. Fertility rates following laparoscopic artificial insemination (LAI) using frozen-thawed …
Electrolyte And Ascorbic Acid Supplementation For Heat Exposed Broilers In Thailand, Chackrit Nuengjamnong, Kris Angkanaporn
Electrolyte And Ascorbic Acid Supplementation For Heat Exposed Broilers In Thailand, Chackrit Nuengjamnong, Kris Angkanaporn
The Thai Journal of Veterinary Medicine
The objective of this study was to compare the effects of ascorbic acid and mixed electrolytes on growth performance, ammonia emission and serum antioxidant enzyme activity in broilers during summer period. Two hundred and ten male and female Arbor Acre broilers during grower-finisher period (22-42 days old) were allocated into 3 groups with different additives in drinking water: 1,000 ppm ascorbic acid (T1); 2,000 ppm mixed electrolytes containing only Na+, K+ and HCO3- (T2) and none (T3 serving as control group). The experiment was conducted in an open broiler house during the summer period between April and May. Results indicated …
Inhibitory Effects Of Aluminosilicates On Lead Acetate Toxicity In Selected Organs Of Broilers, Dejan Prvulović, Danijela Kojić, Milan Popović, Gordana Grubor-Lajšić
Inhibitory Effects Of Aluminosilicates On Lead Acetate Toxicity In Selected Organs Of Broilers, Dejan Prvulović, Danijela Kojić, Milan Popović, Gordana Grubor-Lajšić
The Thai Journal of Veterinary Medicine
The aim of this study was to determine the effect of dietary supplements of lead acetate and aluminosilicates on oxidative status in brain, kidney and duodenum of broiler chickens. In this regard, activity of antioxidative enzymes such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPx), pyrogallol peroxidase (PPx) and lipid peroxidation was determined in examined organs. The experiment was performed with eighty-four broiler chickens of both sexes randomly allotted to four diets including the control group, the Pb group containing 500 mg lead acetate/kg diet, the ATN (Antitoxic nutrient) group with 5 g mixture of aluminosilicates (zeolite and montmorillonite)/kg …
Extended Spectrum Beta-Lactamase Producing Escherichia Coli Isolated From Infected Canines, Parama Hanhaboon, Thachawet Kimprasit, Worrawit Wajjwalku, Patamabhorn Amavisit
Extended Spectrum Beta-Lactamase Producing Escherichia Coli Isolated From Infected Canines, Parama Hanhaboon, Thachawet Kimprasit, Worrawit Wajjwalku, Patamabhorn Amavisit
The Thai Journal of Veterinary Medicine
Escherichia coli strains from dogs with different clinical signs were studied for their antimicrobial sensitivity profiles, extended spectrum beta-lactamase enzymes, and virulent genes. The 364 E. coli isolates originated from the skin (32.48%), urinary tract (31.32%), gastrointestinal tract (16.21%), reproductive system (7.69%), ear (6.05%), respiratory tract (4.94%), and other specimens (1.37%). Using the disk diffusion method, the E. coli were resistant to amoxicillin, amoxicillin-clavulanic acid, azithromycin, cephalexin, ceftriaxone, ciprofloxacin, doxycycline, enrofloxacin, norfloxacin, gentamicin, and sulfamethoxazole/trimethoprim at the rates of 84, 36, 41, 57, 38, 66, 73, 70, 64, 47, and 64%, respectively. Ninety-seven E. coli that were resistant to two …
Postparturient Administration Of Prostaglandin F2 Alpha Facilitates Weaning-To-Service Interval In Primiparous Sows, Atthaporn Roongsitthichai, Padet Tummaruk, Nutthee Am-In
Postparturient Administration Of Prostaglandin F2 Alpha Facilitates Weaning-To-Service Interval In Primiparous Sows, Atthaporn Roongsitthichai, Padet Tummaruk, Nutthee Am-In
The Thai Journal of Veterinary Medicine
The present study was conducted to investigate the effect of exogenous prostaglandin F2 alpha (PGF2α) administration on weaning-to-service interval (WSI) in primiparous sows. A total of 149 primiparous sows, 74 control and 75 treatment sows, were included in the study. The latter sows were intramuscularly injected with PGF2α approximately at 24-48 h after parturition. Results revealed that those treated with PGF2α returned to be in estrus significantly faster than those in the control group (2.6±1.1 vs 4.5±2.1 days, p<0.05), while age at first mating, pregnancy rate, and age at first farrowing were not different from each other. In conclusion, PGF2α could shorten WSI in the primiparous sows as it could induce complete luteolysis and promote uterine involution, resulting in declined non-productive days of the commercial breeding herds.
Histopathological And Immunohistochemical Characterization Of Spontaneous Uterine Leiomyomas In Two Captive Asian Elephants, Kidsadagon Pringproa, Hiroo Madarame, Jiraporn Sritun, Pitikarn Bumpenpol, Paninee Pedsri, Chalermchart Somgird, Chatchote Thitaram
Histopathological And Immunohistochemical Characterization Of Spontaneous Uterine Leiomyomas In Two Captive Asian Elephants, Kidsadagon Pringproa, Hiroo Madarame, Jiraporn Sritun, Pitikarn Bumpenpol, Paninee Pedsri, Chalermchart Somgird, Chatchote Thitaram
The Thai Journal of Veterinary Medicine
Two captive female Asian elephants (Elephas maximus) in Thailand were euthanatized after prolonged recumbency, weakness, and unresponsive treatment. Necropsy revealed multinodular masses of 5-10 cm in diameter which were irregular, large, white and firm in consistency scattered throughout the uterine horns and uteri of both the elephants. Histologically, the tumors displayed mildly pleomorphic, spindle-shaped cells arranged in interlacing pattern interpreted as muscle bundles. The bundles of the muscle cells were interspersed by small amounts of collagen fibers and blood vessels. For immunohistochemistry, the tumor cells showed diffuse positivity for vimentin, desmin, and smooth muscle actin. Based on the pathological results, …
Efficacy Of Autologous Serum As An Adjunct Treatment For A Melting Corneal Ulcer In A Captive Asian Elephant, Thittaya Janyamethakul, Prasit Moleechat, Rinku Gohain, Chalermchat Somgird, Pornsawan Pongsopavijit, Boontarikaan Wititkornkul
Efficacy Of Autologous Serum As An Adjunct Treatment For A Melting Corneal Ulcer In A Captive Asian Elephant, Thittaya Janyamethakul, Prasit Moleechat, Rinku Gohain, Chalermchat Somgird, Pornsawan Pongsopavijit, Boontarikaan Wititkornkul
The Thai Journal of Veterinary Medicine
This is the first case report on the use of autologous serum as an adjunct to successfully treat a melting corneal ulcer in a captive Asian elephant (Elephas maximus). A 40-year-old female Asian elephant was presented with epiphora of the right eye. It had already received treatment for 3 weeks, but had not responded to conventional treatment with antibiotics and analgesic eye drops. Upon examination, a melting corneal ulcer was found. The elephant received a combined treatment that included autologous serum twice a day for 79 days in addition to antibiotic, antifungal and analgesic drugs administered topically. After just 19 …
What Is Your Diagnosis?, Nan Choisunirachon
What Is Your Diagnosis?, Nan Choisunirachon
The Thai Journal of Veterinary Medicine
No abstract provided.
Isolation And Characterization Of Potential Probiotic Bacteria Suitable For Mollusk Larvae Cultures, Ruth Escamilla-Montes, Antonio Luna-González, Ma. Del Carmen Flores-Miranda, Píndaro Álvarez-Ruiz, Jesús Arturo Fierro-Coronado, Ana Claudia Sánchez-Ortiz, José Ávila-Leal
Isolation And Characterization Of Potential Probiotic Bacteria Suitable For Mollusk Larvae Cultures, Ruth Escamilla-Montes, Antonio Luna-González, Ma. Del Carmen Flores-Miranda, Píndaro Álvarez-Ruiz, Jesús Arturo Fierro-Coronado, Ana Claudia Sánchez-Ortiz, José Ávila-Leal
The Thai Journal of Veterinary Medicine
Guts of Crassostrea gigas, C. corteziensis, and Atrina maura were screened for bacilli and lactic acid bacteria with probiotic properties for bivalve mollusks. Characterization of the isolates included Gram stain, morphology, cell arrangement, catalase and hemolytic activity, hydrophobicity, autoaggregation, coaggregation against Vibrio sinaloensis VHPC23, biofilm formation, counting of colony forming units (CFU), NaCl and pH tolerance, enzymatic activity, resistance to antibiotics, antagonism against Vibrio sinaloensis VHPC23, and molecular identification. Three isolates (BCR 4-3, BALCR 5-2, and BALOR 2-3) showed no hemolytic activity, high tolerance to pH and salinity, high adhesion potential, high autoaggregation and coaggregation, biofilm formation, well growth, and …
In Vitro Suppression Against Streptococcal Bacteria And Health-Promoting Effects Of Probiotic Bacillus Polyfermenticus In Tilapia (Oreochromis Niloticus), Mintra Lukkana, Sasibha Jantrakajorn, Janenuj Wongtavatchai
In Vitro Suppression Against Streptococcal Bacteria And Health-Promoting Effects Of Probiotic Bacillus Polyfermenticus In Tilapia (Oreochromis Niloticus), Mintra Lukkana, Sasibha Jantrakajorn, Janenuj Wongtavatchai
The Thai Journal of Veterinary Medicine
In vitro antibacterial activity of Bacillus polyfermenticus (BP) against tilapia streptococcal pathogen was evaluated using the agar dilution method and co-incubation method. Both in vitro tests confirmed the inhibitory effect of B. polyfermenticus on the growth of Streptococcus agalactiae. The suppression of S. agalactiae was observed in the test system containing B. polyfermenticus and the inhibition was more evident in the system with high density of B. polyfermenticus. Subsequently, feeding administration of BP was employed in tilapia broodstocks (12 months old) and fry (37.33 ± 1.37 mg body weight) to examine the effect of BP on health performance. Significantly higher …
Optimization Of Cell Permeabilization For Rapid Detection Of Salmonella In Pork By Fish, Damrongsak Arlai, Sujate Chuanchom, Theerapol Sirinarumitr
Optimization Of Cell Permeabilization For Rapid Detection Of Salmonella In Pork By Fish, Damrongsak Arlai, Sujate Chuanchom, Theerapol Sirinarumitr
The Thai Journal of Veterinary Medicine
Salmonella spp. are one of the major causes of infectious gastroenteritis. The route of infection is usually ingestion of contaminated food. Due to the severity of the disease, it is worthwhile to identify appropriate preventive measures. Bacterial culture method is the gold standard method, but it is time-consuming (at least 4-6 days). Therefore, in this research, the fluorescence in situ hybridization technique (FISH) was optimized for rapid and accurate detection of Salmonella spp. in contaminated pork using a 3¢-end tailing oligonucleotide probe. The optimum digestion condition for cell permeation with lysozyme was 1 mg/ml at 37oC for 3 min. The …
Antibiotic Resistance And Molecular Characterization Of Enterobacter Cancerogenus Isolated From Wild Birds In Taif Province, Saudi Arabia, Aly E. Abo-Amer, Mohammed Y. Shobrak
Antibiotic Resistance And Molecular Characterization Of Enterobacter Cancerogenus Isolated From Wild Birds In Taif Province, Saudi Arabia, Aly E. Abo-Amer, Mohammed Y. Shobrak
The Thai Journal of Veterinary Medicine
Enterobacter infection can cause diseases such as bacteremia, lower respiratory tract infections, skin and soft-tissue infections, urinary tract infections, etc. Enterobacter could be isolated from natural environments. Enterobacter infections can require prolonged hospitalization and powerful and expensive antimicrobial agents. Increase in the number of resistant and multi-resistant strains of bacteria is a major concern to health officials worldwide. Therefore, the objective of the study was to evaluate the prevalence of antibiotic-resistant Enterobacter sp. recovered from wild birds collected in Taif province, Saudi Arabia. Fifty isolates were recovered from cloacal samples of resident wild birds and 32 isolates from cloacal samples …
Effect Of Activated Charcoal-Supplemented Diet On Growth Performance And Intestinal Morphology Of Nile Tilapia (Oreochromis Niloticus), Nopadon Pirarat, Surinton Boonananthanasarn, Laddawan Krongpong, Takayuki Katagiri, Masashi Maita
Effect Of Activated Charcoal-Supplemented Diet On Growth Performance And Intestinal Morphology Of Nile Tilapia (Oreochromis Niloticus), Nopadon Pirarat, Surinton Boonananthanasarn, Laddawan Krongpong, Takayuki Katagiri, Masashi Maita
The Thai Journal of Veterinary Medicine
The effect of tilapia diet supplemented with activated charcoal (AC) on growth performance and intestinal morphology of Nile Tilapia was examined. The fish were divided into four groups: a control group and three AC-supplementation groups (1%, 2% and 3% of AC in diet), and fed approximately 3% of body weight twice a day for 30 days. Growth performance and intestinal villus height were measured. Results revealed that the 2% AC-supplemented diet group was significantly (P<0.05) better in terms of percentage weight gain, specific growth rate (SGR) and feed conversion ratio (FCR), among the experimental groups. The foregut and midgut villus height of the 3% and 2% AC-supplemented diet groups was similar, but was significantly higher than the control group (P<0.05). Consequently, the supplementation of diet with 2% AC was found to be the most suitable for improving the growth performance and intestinal morphology in tilapia.
An Inactivated Vaccine For Prevention And Control Of Inclusion Body Hepatitis In Broiler Breeders, Sucheeva Junnu, Preeda Lertwatcharasarakul, Siriluk Jala, Sakuna Phattanakulanan, Angkasiya Monkong, Sittinee Kulprasertsri, Chotiga Thivalai, Warunya Chakritbudsabong, Kridsada Chaichoun, Thaweesak Songserm
An Inactivated Vaccine For Prevention And Control Of Inclusion Body Hepatitis In Broiler Breeders, Sucheeva Junnu, Preeda Lertwatcharasarakul, Siriluk Jala, Sakuna Phattanakulanan, Angkasiya Monkong, Sittinee Kulprasertsri, Chotiga Thivalai, Warunya Chakritbudsabong, Kridsada Chaichoun, Thaweesak Songserm
The Thai Journal of Veterinary Medicine
Inclusion bodies hepatitis (IBH) in Thailand is caused by Fowl Adenovirus (FAdv) serotype 2. Vertical transmission is the important route for disease outbreak. Biosecurity and vaccination are the major keys for disease control and prevention. Vaccination of broiler breeders results in maternal antibody production that can be transferred to their progenies. Therefore, the aim of present study was to develop an inactivated FAdv serotype 2 vaccine. The FAdv was isolated from liver of infected chickens by inoculation onto chicken embryo liver cells (CEL). Virus purification was performed and then inactivated by Binary Ethylene Imine (BEI). The 107 TCID50 of virus …
In Vitro Modulatory Effects Of Quercetin On Bovine Neutrophil Effector Functions, Phongsakorn Chuammitri, Chiradet Amphaiphan, Panupong Nojit
In Vitro Modulatory Effects Of Quercetin On Bovine Neutrophil Effector Functions, Phongsakorn Chuammitri, Chiradet Amphaiphan, Panupong Nojit
The Thai Journal of Veterinary Medicine
Bovine neutrophils perform numerous effector functions to overcome microbial invasions by utilizing innately-equipped intracellular and extracellular killing mechanisms. In this study, we explored the modulatory effects of quercetin hydrate (QH), an herbal flavonoid compound, on bovine neutrophil functional activities. Isolated neutrophils were incubated with various concentrations of QH (0-100 mM). Neutrophil viability results showed no signs of cytotoxicity (p = 0.33). In other assays, neutrophils were stimulated with a pre-defined concentration of QH (50 mM). Results showed promising effects of QH on enhancing intracellular ROS generation (p < 0.0001) compared to control. Phagocytic activity of fluorescently labeled E.coli was also augmented by the effects of this compound (p = 0.05). However, intracellular killing of live E.coli quantified by MTT assay demonstrated a slight decrease in activity (p = 0.304). Additionally, extracellular killing driven by NET-DNA release was remarkably higher in QH-stimulated cells (p = 0.0004). Supplementation of QH to LPS stimulated neutrophils resulted in significantly decreased pro-inflammatory cytokines, IL-1b (p < 0.0001) and TNF-a gene expression (p = 0.004). Taken together, quercetin could exert its noteworthy actions, both at cellular and molecular levels, at a certain physiological concentration.
Effects Of Immunization Against Gonadotropin Releasing Hormone On Reproductive Functions In Male Rusa Deer (Rusa Timorensis), Orasa Phraluk, Worawidh Wajjwalku, Boripat Siriaroonrat, Orawan Booddee, Nikorn Thongtip
Effects Of Immunization Against Gonadotropin Releasing Hormone On Reproductive Functions In Male Rusa Deer (Rusa Timorensis), Orasa Phraluk, Worawidh Wajjwalku, Boripat Siriaroonrat, Orawan Booddee, Nikorn Thongtip
The Thai Journal of Veterinary Medicine
We performed an evaluation of the effect of gonadotropin releasing hormone (GnRH) vaccine on male rusa deer reproductive function. Mature male rusa deer (Rusa timorensis) were divided into two groups: untreated group (n=3) and treated group (n=7). The treated rusa deer were administered with two ml of the GnRH-protein conjugate vaccine (Improvac®; 200 μg/ml). The vaccine was injected subcutaneously into the treated group three times at four-week intervals (April, May and June) and an additional single dose booster was given after a decrease in GnRH antibody levels was detected (October). Blood testosterone concentrations and GnRH antibody titer were determined before …
Intra-Articular Injections With Allogeneic Dental Pulp Stem Cells For Chronic Osteoarthritis, Ratikorn Bootcha, Jedee Temwichitr, Soontaree Petchdee
Intra-Articular Injections With Allogeneic Dental Pulp Stem Cells For Chronic Osteoarthritis, Ratikorn Bootcha, Jedee Temwichitr, Soontaree Petchdee
The Thai Journal of Veterinary Medicine
Stem cell therapies are rapidly gaining attention as a promising tool for tissue regeneration. A variety of stem cell types have been studied as potential candidates to restore the structure and function of damaged organs or tissues. Recent studies have demonstrated the use of stem cells isolated from dental pulp in dentistry and tissue engineering. In our previous studies we have shown the applications of puppy deciduous teeth stem cells (pDSCs) to restore infarcted myocardium and ulceration of the cornea. For this study, our aim was to provide a novel therapeutic application of allogeneic pDSCs in dogs with chronic osteoarthritis …