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The Thai Journal of Veterinary Medicine

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Full-Text Articles in Veterinary Medicine

The Goat Semen Quality After Being Frozen Using Albumin And Cholesterol Substituted For Egg Yolk In Semen Extender, Terdchai Kaewkesa, Anucha Sathanawongs, Apichart Oranratnachai, Jureerat Sumretprasong Jun 2016

The Goat Semen Quality After Being Frozen Using Albumin And Cholesterol Substituted For Egg Yolk In Semen Extender, Terdchai Kaewkesa, Anucha Sathanawongs, Apichart Oranratnachai, Jureerat Sumretprasong

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the effect of albumin and cholesterol substituted for egg yolk in goat semen extender after frozen-thawed on semen quality and pregnancy rate. The semen was collected from three bucks by using artificial vagina. The semen was divided into 5 groups; in group 1, seminal plasma was removed and 20% egg yolk extender was added (standard), in group 2, 2% egg yolk extender was added into whole semen (control), in group 3, 4 mg/mL albumin extender was added into whole semen, in group 4, 5 mg/mL cholesterol extender was added into whole semen, …


Allergen Sensitization Patterns Of Allergic Dogs: Ige-Microarray Analysis, Yun-Hsia Hsiao, Charles Chen, Ton Willemse Jun 2016

Allergen Sensitization Patterns Of Allergic Dogs: Ige-Microarray Analysis, Yun-Hsia Hsiao, Charles Chen, Ton Willemse

The Thai Journal of Veterinary Medicine

It was the aim of this study to determine sensitization rates to environmental allergens in 50 dogs with allergic dermatitis. Protein microarray of the dogs’ sera was used to identify allergen-specific IgE against 25 most common allergens. Increased IgE levels were most frequently observed for house dust mites (D. pteronyssinus 42.0%, D. farinae 38.0%) and storage mites (22-50%). In particular, the dogs with non-food-induced allergic dermatitis showed higher sensitization rates to mites compared to the dogs with food-induced allergic dermatitis, or a combination thereof. Reactions to insects were, with the exception of one dog sensitized to cockroach, entirely negative, and …


Ameliorative Effects Of Omega-3 Concentrate In Managing Coxofemoral Osteoarthritic Pain In Dogs, Amornrate Sastravaha, Nirut Suwanna, Chayakrit Sinthusingha, Jatuporn Noosud, Atthaporn Roongsitthichai Jun 2016

Ameliorative Effects Of Omega-3 Concentrate In Managing Coxofemoral Osteoarthritic Pain In Dogs, Amornrate Sastravaha, Nirut Suwanna, Chayakrit Sinthusingha, Jatuporn Noosud, Atthaporn Roongsitthichai

The Thai Journal of Veterinary Medicine

The aim of the present study was to investigate the effect of omega-3 concentrate on pain management in dogs with osteoarthritis (OA) at the coxofemoral joint. Totally, 31 dogs with confirmed coxofemoral OA were orthopedically evaluated for pain scores at walk and trot (1-6), joint manipulation (1-3), and examining range of motion (1-4). Besides, blood collection was performed on the first and the last visits. Omega-3 concentrate was orally given to all dogs at one capsule per 10 kgBW since the first visit on a daily basis for four consecutive weeks. On a weekly basis, pain scores were assessed for …


Seroprevalence And Phylogenetic Analysis Of Toxoplasma Gondii From Domestic Cats, Captive Wild Felids, Free-Range Wild Felids And Rats In Certain Regions Of Thailand, Ruangrat Buddhirongawatr, Kridsada Chaichoun, Siriporn Tungsudjai, Ruenruethai Udonsom, Andrew Thompson, Oangart Mahittikorn, Paron Dekumyoy, Yaowalark Sukthana Jun 2016

Seroprevalence And Phylogenetic Analysis Of Toxoplasma Gondii From Domestic Cats, Captive Wild Felids, Free-Range Wild Felids And Rats In Certain Regions Of Thailand, Ruangrat Buddhirongawatr, Kridsada Chaichoun, Siriporn Tungsudjai, Ruenruethai Udonsom, Andrew Thompson, Oangart Mahittikorn, Paron Dekumyoy, Yaowalark Sukthana

The Thai Journal of Veterinary Medicine

Toxoplasmosis is an important zoonotic disease caused by Toxoplasma gondii, an obligated zoonotic apicomplexan parasite. The infection varies according to geographical areas. This work aimed to study the seroprevalence and genotype of T. gondii infection in domestic, captive and free-range wild felids, and in their small mammal prey, rats (Rattus spp). Two hundred and ninety three sera, received from the 4 individual animal groups in Thailand, were tested using the indirect latex agglutination test (ILAT) for specific antibody detection. The nested-PCR for glycerol-3-phosphate (B1) and bradyzoite surface antigen (SAG4) gene detection was used to detect seropositive animals and PCR product …


Electrocardiographic Changes In Dogs With Degenerative Mitral Valve Disease Treated With Pimobendan: A Retrospective Study Of 29 Cases, Sirilak Disatian Surachetpong, Nisarat Boonlue, Pasorn Pupa, Waratsarin Boonsathitanan, Sumanee Rakthaidee, Tanawan Mangklabruks, Siriwan Sakarin Jun 2016

Electrocardiographic Changes In Dogs With Degenerative Mitral Valve Disease Treated With Pimobendan: A Retrospective Study Of 29 Cases, Sirilak Disatian Surachetpong, Nisarat Boonlue, Pasorn Pupa, Waratsarin Boonsathitanan, Sumanee Rakthaidee, Tanawan Mangklabruks, Siriwan Sakarin

The Thai Journal of Veterinary Medicine

The aim of this retrospective study was to evaluate changes in electrocardiograms (ECG) and cardiac arrhythmias in 29 dogs with degenerative mitral valve disease (DMVD) treated with pimobendan chronically. All dogs had normal ECG before pimobendan administration. The dogs were classified according to ECG after pimobendan administration into 2 groups: normal ECG (n=19) and abnormal ECG (n=10). Age, sex, breed, serum alkaline phosphatase, concurrent digoxin administration, total daily dosage of pimobendan and digoxin, duration of pimobendan administration, and ECG parameters including heart rate, P wave, PR interval, QRS complex, and QT interval were analyzed. Cardiac arrhythmias were increasingly found in …


Comparative Proteomic Analysis Of Cow Placentas With Retained Foetal Membranes, Shixin Fu, Yao Liu, Tiantian Nie, Chengyuan Zheng, Xiao Zou, Chunhai Luo, Jianying Liu Jun 2016

Comparative Proteomic Analysis Of Cow Placentas With Retained Foetal Membranes, Shixin Fu, Yao Liu, Tiantian Nie, Chengyuan Zheng, Xiao Zou, Chunhai Luo, Jianying Liu

The Thai Journal of Veterinary Medicine

Retained foetal membrane (RFM) is a common reproductive pathology that harms the health of dairy cows. The aim of this study was to clarify the pathogenesis of RFM and screen for an early clinical diagnostic marker of this disease by using comparative proteomics and bioinformatics. Three RFM placental tissues from three cows and an equal number of normal placental tissues were collected for this study. There were 240 spots showing significant differential expressions of known proteins between the two types of foetal placental tissue samples. Between the two maternal placental tissue samples, there were 214 spots with differentially expressed levels …


Selection Of Reference Genes For Real-Time Polymerase Chain Reaction In Canine Oral Tumor And Cancer, Sirinun Pisamai, Anudep Rungsipipat, Chanin Kalpravidh, Gunnaporn Suriyaphol Jun 2016

Selection Of Reference Genes For Real-Time Polymerase Chain Reaction In Canine Oral Tumor And Cancer, Sirinun Pisamai, Anudep Rungsipipat, Chanin Kalpravidh, Gunnaporn Suriyaphol

The Thai Journal of Veterinary Medicine

Tumors in oral cavity either benign or malignant are often found in dogs. Despite the increasing number of studies of gene expression associated with canine oral cavity diseases, reference gene selection for normalizing target genes has not been performed. The objective of the present study was to identify potential reference genes in canine oral tumors, including epulis, ameloblastoma, oral melanoma, oral squamous cell carcinoma, etc. Suitability of 6 candidate reference genes, beta-actin (ACTB), beta-2-microglobulin (B2M), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), ribosomal subunit L13a (RPL13a), ribosomal protein S5 (RPS5) and ribosomal protein S19 (RPS19), was evaluated with 5 algorithms, geNorm, Normfinder, BestKeeper, the …


Diagnosis Of Pharyngeal Sialocele In A Dog By Ct Scan : A Case Report, Sekkarin Ploypetch, Nan Choisunirachon, Chanin Kalpravidh Jun 2016

Diagnosis Of Pharyngeal Sialocele In A Dog By Ct Scan : A Case Report, Sekkarin Ploypetch, Nan Choisunirachon, Chanin Kalpravidh

The Thai Journal of Veterinary Medicine

A 7-year-old, 10 kg male poodle dog was presented at the Small Animal Teaching Hospital, with the clinical signs of chronic coughing, bad deglutition and severe respiratory distress. On oral examination, a large mass arisen from the throat was found. Because the skull-cervical radiographs revealed only a large soft tissue pharyngeal mass which were difficult to indicate the mass types and the tissue invasiveness, the computed tomography (CT) was subsequently applied. The CT result showed that the mass was an encapsulated hypoattenuation mass (2.5 x 4.3 x 2.4 cm) at the right pharyngeal area without any adjacent soft tissue invasions. …


Ultrasound Diagnosis, Phiwipha Kamonrat Jun 2016

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Protective Efficacy Of Live Lasota Strain Newcastle Disease Virus Vaccine In Layer-Type Chickens, Jiroj Sasipreeyajan, Phornphisut Areeraksakul, Somkid Khanda Jun 2016

Protective Efficacy Of Live Lasota Strain Newcastle Disease Virus Vaccine In Layer-Type Chickens, Jiroj Sasipreeyajan, Phornphisut Areeraksakul, Somkid Khanda

The Thai Journal of Veterinary Medicine

The purpose of this study was to evaluate the protective efficacy of live LaSota strain Newcastle disease virus (NDV) vaccine administered with different routes against a NDV challenge in layer-type chickens. Fifty male layer-type chickens were divided into 3 groups. There were 20 chickens/group for the vaccinated groups (Groups 1 and 2) and 10 chickens for the non-vaccinated control group (Group 3). Chickens in Groups 1 and 2 received the vaccine individually at 4 weeks old by eye drop and oral drop, respectively. All chickens received NDV challenge individually by oral drop at 7 weeks old or 3 weeks post-vaccination …


Genetic Parameters Of Semen Quality Traits And Production Traits Of Pure-Bred Boars In Thailand, Krittaphak Buranawit, Nalinee Imboonta Jun 2016

Genetic Parameters Of Semen Quality Traits And Production Traits Of Pure-Bred Boars In Thailand, Krittaphak Buranawit, Nalinee Imboonta

The Thai Journal of Veterinary Medicine

The objective of this study was to estimate genetic parameters for semen quality and production traits of pure-bred Finnish-Norwegian boars raised in Thailand. Data used in this study were 9,760 records of semen collection and 108 records of performance tests (from 25 Duroc, 59 Landrace, and 24 Yorkshire boars). Semen quality traits considered per collection were semen volume (SV), semen concentration (SC), total sperm (TS), and total abnormality (TA). Data were recorded from 2001 to 2009, except for TA, which was not recorded until 2003. Production measures from each boar were average daily gain (ADG) and backfat thickness (BF). Results …


Identification Of Novel Snps And 10 Bp Deletion Of Ovine Dgat1 Gene And Their Association With Milk Production Traits, Ozge Ozmen, Selim Kul Jun 2016

Identification Of Novel Snps And 10 Bp Deletion Of Ovine Dgat1 Gene And Their Association With Milk Production Traits, Ozge Ozmen, Selim Kul

The Thai Journal of Veterinary Medicine

Acyl coenzyme A: diacylglycerol acyltransferase 1 (dgat1) plays a key role in triacylglycerol synthesis by catalyzing the final committed step in the formation of triglycerides. Moreover, it could be used as a positional marker gene associated with milk production and composition traits. The purpose of the study was to identify genotype frequencies of single nucleotide polymorphisms (SNPs) in ovine dgat1 gene and its possible association with milk traits in dairy sheep breeds. The genetic structures of SNPs were examined by PCR-RFLP and DNA sequencing in three sheep populations. Significant association of dgat1 gene of the intron 16 g.8384T>C loci …


Identification Of Competitive Exclusion And Its Ability To Protect Against Campylobacter Jejuni In Broilers, Thotsapol Thomrongsuwannakij, Rungtip Chuanchuen, Niwat Chansiripornchai Jun 2016

Identification Of Competitive Exclusion And Its Ability To Protect Against Campylobacter Jejuni In Broilers, Thotsapol Thomrongsuwannakij, Rungtip Chuanchuen, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

This study aimed to identify competitive exclusion (CE) isolated from native chickens and organic layers raised under non-antimicrobial usage farms. The protection of CE was tested against Campylobacter jejuni challenges in broilers. Lactobacillus spp., Bacillus spp. and Enterococcus faecium were identified from 50 adult chicken feces of those farms. According to antimicrobial-resistance concerns of European Food Safety Authority (EFSA), the use of CE that is susceptible to antimicrobials is preferred. The numbers of Lactobacillus spp., Bacillus spp. and Enterococcus faecium equal to 195, 93 and 58 strains, respectively, were tested for their minimum inhibitory concentrations (MICs) of 10 antimicrobials. As …


Bactericidal Effects Of Povidone-Iodine And Chlorhexidine Gluconate On Coagulase-Positive Staphylococci, Punpichaya Fungwithaya, Nuvee Prapasarakul Jun 2016

Bactericidal Effects Of Povidone-Iodine And Chlorhexidine Gluconate On Coagulase-Positive Staphylococci, Punpichaya Fungwithaya, Nuvee Prapasarakul

The Thai Journal of Veterinary Medicine

We aimed to evaluate the in vitro bactericidal efficacy of two routine antiseptics used in veterinary hospital, povidone-iodine and chlorhexidine gluconate in isopropanol (CGI),against canine coagulase-positive staphylococci (CoPS). Twenty CoPS were divided into 5 methicillin-resistant Staphylococcus pseudintermedius (MRSP) and 5 isolates each of S. pseudintermedius, S. aureus and S. schleiferi subsp. coagulans defined as methicillin-susceptible CoPS (MSCoPS). The bactericidal efficacy was determined by broth microdilution according to European Standard EN 1656:2000 at concentrations of 0.1, 1 and 10% PI and 0.5, 1 and 2% CGI for 15s, 30s, 45s, 1 min, 3 min and 5 min, respectively. There was no …


Quantitative Method For Detecting Vibrio Parahaemolyticus Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Saburo Ohkawa, Yukio Morita, Sumalee Boonmar Jun 2016

Quantitative Method For Detecting Vibrio Parahaemolyticus Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Saburo Ohkawa, Yukio Morita, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

This is the first report on the quantitative method for detecting Vibrio parahaemolyticus using the Bio-Theta DOXTM system. Seven V. parahaemolyticus strains and 23 non-V. parahaemolyticus strains were assessed. The rate of positive detection of Vibrio in samples of the 7 V. parahaemolyticus strains at high concentration (105 cfu/ml), medium concentration (103 cfu/ml), and low concentration (10 cfu/ml) was 100%. All 7 V. parahaemolyticus strains displayed good linear calibration curves for detection time relative to bacterial count (r > 0.993). The mean negative rate for the high-concentration (106 cfu/ml) non-V. parahaemolyticus samples was 65.2% (15/23) and that for the medium-concentration (103 …


Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Saikaew Sutayatram Jun 2016

Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Saikaew Sutayatram

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich Jun 2016

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Nan Choisunirachon Jun 2016

What Is Your Diagnosis?, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.


Effect Of Antibiotic Growth Promoters On Anti-Oxidative And Anti-Inflammatory Activities In Broiler Chickens, Krittika Kabploy, Nuntavan Bunyapraphatsara, Noppawan Phumala Morales, Nuanchan Paraksa Mar 2016

Effect Of Antibiotic Growth Promoters On Anti-Oxidative And Anti-Inflammatory Activities In Broiler Chickens, Krittika Kabploy, Nuntavan Bunyapraphatsara, Noppawan Phumala Morales, Nuanchan Paraksa

The Thai Journal of Veterinary Medicine

The current study was conducted to evaluate the effects of sub-therapeutic antibiotics (avilamycin and flavophospholipol) in animal feed on anti-oxidative and anti-inflammatory properties in broilers to understand the mechanisms of AGPs. Four hundred and fifty six 1-day-old broilers (ROSS 308) were divided into 3 groups with 4 replications. Three experimental diets composed of a corn-soybean meal based diet supplemented with 0, 10 mg/kg avilamycin and 5 mg/kg flavophospholipol were randomly provided to the broilers for 42 days, respectively. Results showed that the AGPs significantly decreased TBA-MDA adduct in serum as well as villi tissue of the broilers, especially flavophospholipol. However, …


Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Sirilak Disatian Surachetpong, Pakit Boonpala Mar 2016

Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Sirilak Disatian Surachetpong, Pakit Boonpala

The Thai Journal of Veterinary Medicine

No abstract provided.


Use Of Different Models For Estimation Of Genetic Parameters And Genetic Trends Of Performance Test Traits Of Gilts, Dragomir Lukač Mar 2016

Use Of Different Models For Estimation Of Genetic Parameters And Genetic Trends Of Performance Test Traits Of Gilts, Dragomir Lukač

The Thai Journal of Veterinary Medicine

The aims of this research were to compare estimates of variance components using different animal models and to determine the most suitable mixed model for estimating genetic parameters and genetic trends for traits in performance test of gilts of different breeds using REML. A total of 73129 gilts of four genotypes in the period of 2009 to 2013 were included in the analyses. Four mixed models were constructed. Information criterion of Akaike (AIC) and Bayesian information criterion (BIC) were used to suggest which model is an adequate model for evaluation of genetics parameters. With the introduction of certain factors in …


Prevalence Of Subclinical Laminitis And Its Effects On Reproductive Performance In Lactating Cows In Thailand, Suvaluk Seesupa, Kwankate Kanistanon, Rittichai Pilachai, Suneerat Aiumlamai Mar 2016

Prevalence Of Subclinical Laminitis And Its Effects On Reproductive Performance In Lactating Cows In Thailand, Suvaluk Seesupa, Kwankate Kanistanon, Rittichai Pilachai, Suneerat Aiumlamai

The Thai Journal of Veterinary Medicine

The objectives of this study were to investigate the prevalence of subclinical laminitis and to evaluate its effects on reproductive performances in dairy cows in Thailand. Ninety-eight lactating cows from 22 smallholder dairy farms and 138 lactating cows from one large scale dairy farm were assessed for subclinical laminitis by evaluating lesions after hoof trimming. Hemorrhagic lesions of the sole and white line area were evaluated. Any appearance of sole hemorrhage or white line hemorrhage scoring 2 on one or more claws was defined as a case of subclinical laminitis. Reproductive data were recorded and analyzed using subclinical laminitis as …


Activated Partial Thromboplastin Time (Aptt) In Dogs Tested By In-House Crude Cephalin From Canine Brain, Sarawut Yama, Am Chaimongkhol, Somsak Channoi, Sakhorn Pornprasert, Wanna Suriyasathaporn Mar 2016

Activated Partial Thromboplastin Time (Aptt) In Dogs Tested By In-House Crude Cephalin From Canine Brain, Sarawut Yama, Am Chaimongkhol, Somsak Channoi, Sakhorn Pornprasert, Wanna Suriyasathaporn

The Thai Journal of Veterinary Medicine

This research aimed to identify the reference value of activated partial thromboplastin time (APTT) in normal dogs using crude cephalin prepared in-house from canine brain, and to examine its efficacy in detecting disorders of coagulation factors. A total of 105 blood samples were collected from normal dogs between January-May 2010. The APTT value was found to be 10.43-19.08 seconds and significantly correlated with the APTT value of a test using a commercial reagent (Pearson’s correlation, r = 0.9923, p < 0.001). On testing the reagent’s efficacy in detecting disorders of coagulation factors by using cryopoor plasma, it was discovered that the APTT value ranged from 24.50 to 33.44 seconds, and the mean value of the APTT by means of cryopoor plasma was significantly greater than that of normal dogs (p < 0.001). The aforementioned APTT value significantly correlated with that obtained by means of a commercial reagent (Pearson’s correlation, r = 0.9984, p < 0.001). From this research, it can be confirmed that in-house crude cephalin can be substituted for commercial crude cephalin. Besides, the obtained reference range can further be applied in veterinary hematology laboratory.


Evaluation Of Pain And Prostate Volume Of Dogs Treated With Intra-Prostatic Injection Of Botulinum Toxin Type A, Pintira Thiangthientham, Kaywalee Chatdarong, Suppawiwat Ponglowhapan Mar 2016

Evaluation Of Pain And Prostate Volume Of Dogs Treated With Intra-Prostatic Injection Of Botulinum Toxin Type A, Pintira Thiangthientham, Kaywalee Chatdarong, Suppawiwat Ponglowhapan

The Thai Journal of Veterinary Medicine

Recently, alternative medical treatment of benign prostatic hyperplasia (BPH) using intra-prostatic injection of botulinum toxin type A (BT-A) has been reported in men and mice. However, in terms of animal welfare, it remains questionable when applied to clinical use. This study aimed at evaluating the effects of intra-prostatic injection of BT-A (Botox, Allergan, USA) in dogs. Six healthy intact males with no clinical signs of BPH received 100 units of BT-A by ultrasound-guided transabdominal intra-prostatic injection (50 units of each prostatic lobe). Pain scoring, evaluation of white blood cell counts, cortisol concentrations, and observation of urination behaviour were recorded before …


What Is Your Diagnosis?, Nan Choisunirachon Mar 2016

What Is Your Diagnosis?, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.


Seroprevalence Of Influenza A In Domestic Dogs In Thailand, 2013, Sunicha Chanvatik, Ratanaporn Tangwangvivat, Supassama Chaiyawong, Duangduean Prakairungnamthip, Ranida Tuanudom, Aunyaratana Thontiravong, Alongkorn Amonsin Mar 2016

Seroprevalence Of Influenza A In Domestic Dogs In Thailand, 2013, Sunicha Chanvatik, Ratanaporn Tangwangvivat, Supassama Chaiyawong, Duangduean Prakairungnamthip, Ranida Tuanudom, Aunyaratana Thontiravong, Alongkorn Amonsin

The Thai Journal of Veterinary Medicine

Canine influenza viruses (CIVs) have been reported in dogs worldwide. Due to the relatively high risk of influenza A virus (IAV) infections in dogs and humans through dog-human interface, the seroprevalence of IAVs in dogs in Thailand was surveyed. A serological survey of IAV infections in dogs in Thailand was conducted from December 2012 to November 2013 using ELISA assays for detecting anti-nucleoprotein (NP) antibodies (NP-ELISA) and hemagglutination inhibition (HI) tests for detecting HA-specific antibodies using canine H3N2 (CIV-H3N2), human pandemic H1N1 2009 (Human-pH1N1) and human seasonal H3N2 (Human-H3N2) as viral antigens. A total of 9,891 serum samples were obtained …


Association Between Cephalexin Administration And Emergence Of Methicillin-Resistant Coagulase-Positive Staphylococci (Mrcops) In Dogs, Punpichaya Fungwithaya, Pattrarat Chanchaithong, Nathita Phumthanakorn, Passana Muaungkong, Pitikarn Bumpenpol, Mutchamon Kaewparuehaschai, Chanwit Tribudharat, Nuvee Prapasarakul Mar 2016

Association Between Cephalexin Administration And Emergence Of Methicillin-Resistant Coagulase-Positive Staphylococci (Mrcops) In Dogs, Punpichaya Fungwithaya, Pattrarat Chanchaithong, Nathita Phumthanakorn, Passana Muaungkong, Pitikarn Bumpenpol, Mutchamon Kaewparuehaschai, Chanwit Tribudharat, Nuvee Prapasarakul

The Thai Journal of Veterinary Medicine

The potential transmission of methicillin-resistant coagulase-positive staphylococci (MRCoPS) between dogs and human has been noted as of potential public health concern. The current study aimed to determine the emergence of methicillin-resistant coagulase-positive staphylococci (MRCoPS) in dogs after oral administration of cephalexin. Skin swabs from 38 dogs without a history of antibiotic exposure were collected before drug administration (pre-treatment dogs) and during drug administration within one month (treatment dogs). A total of 196 CoPS were isolated from the nose, perineum and skin lesions. Fewer MRCoPS were isolated from the pre-treatment dogs (7.89%) than from the treatment dogs (p<0.001). Methicillin-resistant Staphylococcus (S.) schleiferi subsp. coagulans (MRSSc) were only recovered from the treatment dogs, whereas methicillin-resistant S. pseudintermedius (MRSP) were found in both groups. Overall, a high incidence of MRSP was found since the first week after administration. The nose and perineum were confirmed as the most common site of carriage of MRCoPS rather than the skin lesions. In conclusion, the oral cephalexin administration was associated with the emergence of MRCoPS on dog skin, a potential source of contamination to humans.


Genetic Detection And Identification Of Chlamydophila Psittaci In Captive Psittacine Birds In Thailand, Parut Suksai, Benchapol Lorsunyaluck, Patcharida Dittawong, Pornchai Sanyathitiseree, Preeda Lertwatcharasarakul Mar 2016

Genetic Detection And Identification Of Chlamydophila Psittaci In Captive Psittacine Birds In Thailand, Parut Suksai, Benchapol Lorsunyaluck, Patcharida Dittawong, Pornchai Sanyathitiseree, Preeda Lertwatcharasarakul

The Thai Journal of Veterinary Medicine

Chlamydophilosis is an important, worldwide zoonosis caused by Chlamydophila psittaci. The aim of this study was to detect C. psittaci on choanal and cloacal swabs which were collected from 178 captive psittacine birds (23 different species) from eight provinces in Thailand. ompA gene detection using nested PCR showed that 7.87% (n=14) were positive for C. psittaci. The C. psittaci detection from two site collections (choanal and cloacal swabs) using nested PCR was more sensitive than from one site collection. Most positive samples (n=11) were from asymptomatic adult birds and three other positive birds showing clinical signs were juvenile birds. As …


Control Of Estrus In Gilts And Primiparous Sows, Roy N. Kirkwood, Fabio De Rensis Mar 2016

Control Of Estrus In Gilts And Primiparous Sows, Roy N. Kirkwood, Fabio De Rensis

The Thai Journal of Veterinary Medicine

The primary controller of weaned pig output is the successful breeding of enough females in the breeding week. The number of females available for breeding is dependent on the number of sows weaned sows and service-ready gilt availability. Primiparous (P1) sows often have longer wean-estrus intervals (WEI) and increased anestrus; ensuring sufficient gilts and sows are available may require control of estrus. Stimulating estrus requires appropriate boar contact or when this is not sufficient, administration of gonadotrophins. If gonadotrophins are used expect estrus 4 to 6 d later and it is usual to breed at the induced estrus. If there …


Comparison Of Effects Of Different Antioxidants Supplemented To Long-Term Extender On Boar Semen Quality Following Storage At 17ºc, Thevin Vongpralub, Pachara Thananurak, Chamaiporn Sittikasamkit, Phirawit Chuawongboon, Monchai Duangjinda, Wuttigrai Boonkum, Vibuntita Chankitisakul Mar 2016

Comparison Of Effects Of Different Antioxidants Supplemented To Long-Term Extender On Boar Semen Quality Following Storage At 17ºc, Thevin Vongpralub, Pachara Thananurak, Chamaiporn Sittikasamkit, Phirawit Chuawongboon, Monchai Duangjinda, Wuttigrai Boonkum, Vibuntita Chankitisakul

The Thai Journal of Veterinary Medicine

The use of antioxidants as semen additives in the extender has been proposed to improve sperm function during cool storage. The objective of this study was to compare the effects of different antioxidant supplementation in extender on boar semen quality during storage for 10 days at 17ºC. Semen samples (n=18) were divided into 10 aliquots and diluted with Reading extender supplemented with three different concentrations of water-soluble glutathione, L-carnitine, and vitamin E analogue Trolox (0.1, 1 and 5mM). All samples were stored in a refrigerator at 17ºC for 10 days. Sperm motility was assessed at Days 1, 4, 7 and …