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Full-Text Articles in Veterinary Medicine
Effects Of Fingerroot (Boesenbergia Rotunda) Powder On Egg Performance, And Quality, Egg Yolk Malondialdehyde In Laying Hens, Miftah Nurfarid
Effects Of Fingerroot (Boesenbergia Rotunda) Powder On Egg Performance, And Quality, Egg Yolk Malondialdehyde In Laying Hens, Miftah Nurfarid
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to determine the effect of fingerroot powder supplementation on the performance of laying hens, egg quality, and malondialdehyde in the egg yolk. A total of 192 Hy-Line brown laying hens aged 43 weeks were divided into four treatment groups with 48 hens in each group (8 replicates; 6 birds each). The treatments were the control (basal diet), basal diet supplemented with vitamin E at 250 mg/kg, basal diet plus 20 g fingerroot powder/kg, and basal diet plus 40 g fingerroot powder/kg. Performance parameters were analyzed in the following two experimental period: 1 to 3 week and 4 …
Effects Of Supplemented Intra- And Extra-Cellular Trehalose On Cryopreservation Of Feline Oocytes, Saengtawan Arayatham
Effects Of Supplemented Intra- And Extra-Cellular Trehalose On Cryopreservation Of Feline Oocytes, Saengtawan Arayatham
Chulalongkorn University Theses and Dissertations (Chula ETD)
Study 1 aimed at examining the effects of different stages of oocyte maturation and trehalose concentrations on freezing ability and developmental capability of feline oocytes. The immature and matured feline oocytes were cryopreserved and thawed. They were assessed for developmental competence. The effects of different concentrations of trehalose (0, 0.125, 0.25, 0.5, 1 M) on the freezing ability of feline oocytes were tested. The cryopreservation generally induced poor embryo development, while immature oocytes were more susceptible to cryodamage compared with the matured oocytes (P<0.05). Incubation of oocytes with high concentrations of trehalose (0.5, 1 M) significantly reduced the developmental competence. Optimal concentrations of trehalose during cryopreservation of immature and matured oocytes were different (0.125 M and 0.25 M, respectively). It is concluded that stages of meiotic maturation affected the freezing ability of feline oocytes. Optimal concentration of trehalose was differently required for immature and matured feline oocytes. Study 2 aimed at examining the effect of membrane-permeable trehalose on the freezing ability of feline oocytes matured in vitro. intracellular trehalose (trehalose hexaacetate; Tre-(OAc)6) was synthesized from trehalose precursor and subjected to spectroscopic characterization. The membrane permeability of the Tre-(OAc)6 was investigated by incubating oocytes with different concentrations of Tre-(OAc)6 (3, 15, and 30 mM) and optimum concentration and the toxicity of Tre-(OAc)6 were subsequently assessed. The effects of Tre-(OAc)6 on freezing ability in terms of apoptotic gene expression and developmental competence of in-vitro matured oocytes were examined, respectively. The Tre-(OAc)6 permeated into the ooplasm of cat oocytes in a dose- and time-dependent manner. The highest concentration of intracellular trehalose was detected when the oocytes were incubated for 24 h with 30 mM Tre-(OAc)6. For the toxicity test, incubation of oocytes with 3 mM Tre-(OAc)6 for 24 h did not affect maturation rate and embryo development. However, high doses of Tre-(OAc)6 (15 and 30 mM) significantly reduced maturation and fertilization rates (P < 0.05). In addition, frozen-thawed oocytes treated with 3 mM Tre-(OAc)6 significantly upregulated anti-apoptotic (BCL-2) gene expression compared with the control (0 mM) and other Tre-(OAc)6 concentrations (15 and 30 mM). Oocyte maturation in the presence of 3 mM Tre-(OAc)6 prior to cryopreservation significantly improved oocyte developmental competence in terms of cleavage and blastocyst rates when compared with the control group (P < 0.05). Our results lead us to infer that increasing the levels of intracellular trehalose by Tre-(OAc)6 during oocyte maturation improves the freezing ability of feline oocytes, albeit at specific concentrations. Study 3 aimed at examining the effects of combination of intra-cellular (Tre-(OAc)6) and extra-cellular (α,α-trehalose) trehalose on freezing ability of feline oocytes. Optimal concentrations of Tre-(OAc)6 (0, 1.5, and 3 mM) and trehalose (0, 0.125, 0.25, and 0.5 M) were investigated for cryopreservation of matured feline oocytes. The combination of the two optimal concentrations were subsequently examined. The results showed that 3 mM of Tre-(OAc)6 improved post-thawed developmental competence, while extra-cellular trehalose at 0.25 M significant enhanced freezing ability of feline oocytes (P<0.05). The combination of intra- and extra-cellular trehalose significantly improved developmental capability of frozen-thawed matured feline oocytes compared to non-trehalose control.
Metaproteomic Analysis Of Gut Resistome In The Cecal Microbiota Of Fattening Pigs Raised Without Antibiotics, Pamornya Buthasane
Metaproteomic Analysis Of Gut Resistome In The Cecal Microbiota Of Fattening Pigs Raised Without Antibiotics, Pamornya Buthasane
Chulalongkorn University Theses and Dissertations (Chula ETD)
Improper use of antibiotics in swine could reduce commensal bacteria and possibly increase pathogen infections via the gut resistome. This study aimed to compare the metaproteomic profiles of gut resistome and related metabolism in the cecal microbiota of fattening pigs raised under antibiotic-free (ABF) conditions with those of ordinary industrial pigs (controls, CTRL). The top three relative abundant microbials in both groups were Escherichia coli, Ruminococcus and Lactobacillus, followed by Bacteroides and Bifidobacterium. E. coli, Lactobacillus and Bacteroides were found to be increased in the CTRL group, whereas Ruminococcus and Clostridium were greater in the ABF group. The highest abundance …
The Effect Of High Dose Ascorbic Acid And High Dcad Administration On Oxidative Stress And Mammary Gland Function Of Dairy Goat Fed Under Tropical Condition, Sapon Semsirmboon
The Effect Of High Dose Ascorbic Acid And High Dcad Administration On Oxidative Stress And Mammary Gland Function Of Dairy Goat Fed Under Tropical Condition, Sapon Semsirmboon
Chulalongkorn University Theses and Dissertations (Chula ETD)
High ambient temperature (HTa) increased the respiration rate (RR) and rectal temperature (Tr) of dairy goats. The excess response resulted in acid-base imbalance, electrolyte imbalance, and systemic oxidative which could indirectly affect the mammary gland. Besides, HTa did directly alter cell function, and this has been proposed to be mediated by oxidative stress. We previously showed that high dietary cation and anion differences (DCAD) could decrease the HTa effect. In dairy cows, a high dose ascorbic acid (Asc) has been used to decrease oxidative stress. Then, It was hypothesized whether mammary gland function could be altered when the HTa was …
Microencapsulation Of Basil Oil And Its Effect On Replacement Of Antibiotic Growth Promoter, Antioxidant Status And Gut Functions Of Broilers, Sureerat Thuekeaw
Microencapsulation Of Basil Oil And Its Effect On Replacement Of Antibiotic Growth Promoter, Antioxidant Status And Gut Functions Of Broilers, Sureerat Thuekeaw
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this work were 1) to study the preparation and characterization of microencapsulated basil oil (MBO), 2) to determine the stability of MBO against simulated gastrointestinal tract and storage 3) to investigate the antimicrobial and antioxidant properties of MBO and 4) to evaluate the supplementation of MBO on growth performance, antioxidant status, ileal digestibility and intestinal morphology in broiler chickens. The present work demonstrates the use of double-layered polymers (chitosan, CS and sodium alginate, SA) with opposite charges leading to a dual pH responsiveness. The double-layered microcapsule was studied on physicochemical characteristics, the swelling performance as well as …
Molecular Characterization And Antimicrobial Susceptible Profiles Of Thai Mycoplasma Synoviae And Immune Response Chickens Received Live Mycoplasma Synoviae Vaccine, Kriengwich Limpavithayakul
Molecular Characterization And Antimicrobial Susceptible Profiles Of Thai Mycoplasma Synoviae And Immune Response Chickens Received Live Mycoplasma Synoviae Vaccine, Kriengwich Limpavithayakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mycoplasma synoviae infection in chickens which has been increasing worldwide is mostly controlled by pathogen-free flocks’ maintenance, medication in infected flocks and vaccination in high-risk flocks. The effective control strategy requires the information of diagnostic assays for detecting and differentiating M. synoviae strains, the understanding of immune response mechanisms in vaccinated chickens, and the reliable drug susceptible evidents for making decision in antimicrobial usage. This study aimed to develop a convenient assay for evaluation of quality or uniformity of vaccination with live MS-H vaccine, to characterize the immune response mechanisms in chickens receiving 3 different vaccination programs immunizing single dose …
Role Of Kca3.1 Channel On Renal Fibrosis In Doxorubicin-Induced Feline Kidney Cell Line, Penpicha Kuedbantakien
Role Of Kca3.1 Channel On Renal Fibrosis In Doxorubicin-Induced Feline Kidney Cell Line, Penpicha Kuedbantakien
Chulalongkorn University Theses and Dissertations (Chula ETD)
Feline renal fibrosis is the most common outcome of chronic kidney disease in cats. Nowadays, there is no specific treatment and the mechanism of fibrosis in cats is still unknown. Intermediate conductance Ca2+ activated K+ channel (KCa3.1) plays an important role in novel therapy for organ fibrosis in many species. Transforming growth factor- β (TGF-β)/Smad signaling pathway and KCa3.1 has been shown to relate to renal fibrosis. This study aimed to investigate the relationship between KCa3.1 and feline renal fibrosis via TGF-β/Smad signaling pathway. CRFK cultures were induced with doxorubicin (DOX) to generate feline renal fibrosis model. Triarymethane-34 (TRAM-34), selective …
Effects Of Microplastics On Gene Expression To Nonspecific Immune System In Pacific White Shrimp (Litopenaeus Vannamei)., Songsak Niemcharoen
Effects Of Microplastics On Gene Expression To Nonspecific Immune System In Pacific White Shrimp (Litopenaeus Vannamei)., Songsak Niemcharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Microplastics pollution effects are increasingly observed as new studies emerge in different ecosystem and organismal levels. It is becoming clear that exposure to different types of microplastic can impair the nonspecific immune system of crustaceans. Therefore, this study investigated the impact of high-density polyethylene microplastic particles (HDPE-MP) in feed on nonspecific immune system gene expression of Pacific white shrimp (Litopenaeus vannamei). The LC50 at day 28 of HDPE-MP ingestion was 3.074% w/w of food. Superoxide dismutase (SOD) and the Glutathione peroxidase (GPx) gene were upregulated significantly in groups supplemented with 0.1 and 0.5% of HDPE-MP and then down-regulated significantly after …
Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott
Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott
Chulalongkorn University Theses and Dissertations (Chula ETD)
PEDV and PDCoV are recognized as important pathogens causing enteric disease in swine herds worldwide. During 2014-2015, several outbreaks of PEDV occurred in many provinces in Thailand, and the clinical signs were more severe than those of classical PEDV in Thailand during 2008-2012. Therefore, the new introduction of PEDV or mutated virus strains was suspected. In 2015, PDCoV was also reported in Thailand, making the situation of enteric disease in the Thai swine industry worse. Since genetic classification studies of PEDV and PDCoV had never been done in Thailand before, this study aimed to investigate the genetic diversity of PEDV …
Estimating Canine Distemper Virus Exposure In Captive Carnivores In Thai Zoos Using A Pseudotype-Based Serum Neutralization Test, Erngsiri Kaewkhunjob
Estimating Canine Distemper Virus Exposure In Captive Carnivores In Thai Zoos Using A Pseudotype-Based Serum Neutralization Test, Erngsiri Kaewkhunjob
Chulalongkorn University Theses and Dissertations (Chula ETD)
Despite its name, Canine distemper virus (CDV) infection is not restricted to canids but extends to several species of the order Carnivora. The extent of CDV exposure in captive carnivores in Thailand has never been estimated before. Therefore, the objective of this study is to estimate CDV exposure in captive carnivores in Thai zoos using a pseudotype-based neutralization assay (SNT). A total of 264 archived serum samples collected between 2005 and 2020 from captive carnivore species from 6 zoos of the Zoological Park Organization of Thailand (ZPOT) (211 samples) and Tiger Kingdom (TK) (53 samples) were tested in this study. …
Situation Analysis And Kap On Antimicrobial Use And Resistance Among Veterinarians And Broiler Poultry Farmers Of Nepal, Manoj Kumar Shahi
Situation Analysis And Kap On Antimicrobial Use And Resistance Among Veterinarians And Broiler Poultry Farmers Of Nepal, Manoj Kumar Shahi
Chulalongkorn University Theses and Dissertations (Chula ETD)
The primary goal of this research was to assess the situation analysis and knowledge, attitude, and practices (KAP) of poultry practitioner veterinarians (PPV) and broiler poultry farmers (BPF) regarding antimicrobial use (AMU) and antimicrobial resistance (AMR) in Nepal. The study area covered approximately 88.1% of Nepal's poultry population. A total of 327 PPV from 56 districts and 500 BPF from 40 districts of seven provinces participated. AMU situation and demographic information were collected and analyzed using descriptive statistics and logistic regression analysis to determine associations between variables. Most PPV were male (85.0%) with a mean age of 31.9±7.8 years. Half …
Associations Of Feline Paramyxoviruses With Pathological Lesions In Cat Kidneys, Aisyah Nikmatuz Zahro
Associations Of Feline Paramyxoviruses With Pathological Lesions In Cat Kidneys, Aisyah Nikmatuz Zahro
Chulalongkorn University Theses and Dissertations (Chula ETD)
Paramyxoviruses in cats composing of feline paramyxovirus (FPaV), feline morbillivirus genotype 1 (FeMV-1), and feline morbillivirus genotype 2 (FeMV-2). These viruses had been recently suggested to be associated with kidney disease in cats. However, pathological consequence of infection is remained restricted. This study aimed to investigate the association of paramyxovirus infection and pathological consequence in cats’ kidney. Renal fresh tissues were collected from routine necropsy in Department of Pathology, Faculty of Veterinary Science, Then, tissues were histopathologically categorized into normal group and pathologic group. To screen paramyxovirus infection, reverse-transcription polymerase chain reaction (RT-PCR) assays using Res-Mor-Hen pan-primer set and specific …
The Infection Dynamics, Co-Infection, Multi-Gene Phylogeny, And Molecular Screening Of Plasmodium Caprae Infection In Goats And Mosquito Species, Hoang Lan Anh Nguyen
The Infection Dynamics, Co-Infection, Multi-Gene Phylogeny, And Molecular Screening Of Plasmodium Caprae Infection In Goats And Mosquito Species, Hoang Lan Anh Nguyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Ungulate malaria parasites, including Plasmodium caprae that infects the domestic goats, have received little attention. There are only few studies about the prevalence and mitochondrial genome analyses. This study, therefore, aimed to investigate the natural infection dynamics of P. caprae and its mosquito vectors. Besides, genome analyses based on nuclear genes of P. caprae and other related haemosporidian parasites were also carried out to obtain an in depth understanding about their evolutionary history and origin. A total of 423 goat blood samples were collected during rainy seasons from 2018 to 2021 to assess the malaria infection status. In addition, 1,019 …
Anti-Proliferative And Apoptotic Effect Of Cannabinoids On Human Pancreatic Ductal Adenocarcinoma Xenograft In Balb/C Nude Mice Model, Quang Trung Le
Anti-Proliferative And Apoptotic Effect Of Cannabinoids On Human Pancreatic Ductal Adenocarcinoma Xenograft In Balb/C Nude Mice Model, Quang Trung Le
Chulalongkorn University Theses and Dissertations (Chula ETD)
Human pancreatic ductal adenocarcinoma (PDAC) is a highly malignant and lethal tumor of the exocrine pancreas. Cannabinoids extracted from the hemp plant Cannabis sativa have been suggested as a cancer therapy due to their anti-tumor effects on several human tumors. However, the anti-tumor effect of cannabinoids on human PDAC is still understudied. Therefore, the objective of the current study is to study the inhibition of proliferation and the induction of apoptosis potencies of cannabinoids in PDAC xenograft nude mice model. The human PDAC cell line (Capan-2) was subcutaneously injected into twenty-five nude mice. After tumor reached 200 mm3 in volume, …
In Vitro Inhibitory Effects Of Turmeric Extracts Against Biofilm Formation And Virulence Of Flavobacterium Oreochromis, Sirawich Ngernson
In Vitro Inhibitory Effects Of Turmeric Extracts Against Biofilm Formation And Virulence Of Flavobacterium Oreochromis, Sirawich Ngernson
Chulalongkorn University Theses and Dissertations (Chula ETD)
Flavobacterium oreochromis is an opportunistic bacteria and causative agent of columnaris disease that affects freshwater fish worldwide. To initiate infection, the bacteria are required to attach and form biofilm on the fish's skin. The bacteria cells in the biofilm then produce toxins that can degrade fish connective tissue, resulting in lesions and ulcers. This study aimed to evaluate the efficacy of turmeric (Curcuma longa L.) extract by three organic solvents (hexane, ethanol, and methanol) in biofilm inhibition and modulation of biofilm-associated genes of F. oreochromis. Turmeric extracts by three different organic solvents were tested for their ability to inhibit the …
Molecular Characterization Of Clinostomum Spp. Infecting Cultured Snakeskin Gourami (Trichopodus Pectoralis) In Thailand, Sk Injamamul Islam
Molecular Characterization Of Clinostomum Spp. Infecting Cultured Snakeskin Gourami (Trichopodus Pectoralis) In Thailand, Sk Injamamul Islam
Chulalongkorn University Theses and Dissertations (Chula ETD)
The digeanean Clinostomum spp. is a significant parasitic pathogen of freshwater fish. It has low host specificity and a wide distribution geographically. Morphological and molecular data analyses have been performed on Clinostomum to assess its diversity. However, the molecular occurrence of Clinostomum spp. in aquaculture in Thailand has yet to be reported. Snakeskin gourami (Trichopodus pectoralis), a freshwater fish cultured in Thailand, has significant economic importance. Herein, the study focuses on describing a digenean Clinostomum in the abdominal cavity of T. pectoralis and reports the prevalence of this parasite from central Thailand. Two hundred and sixty-two 4 to 8- month-old …
Seroprevalence Of Lawsonia Intracellularis Antibody Among Swine Herds In Thailand, December 2021-March 2022, Thanh Nguyen Che
Seroprevalence Of Lawsonia Intracellularis Antibody Among Swine Herds In Thailand, December 2021-March 2022, Thanh Nguyen Che
Chulalongkorn University Theses and Dissertations (Chula ETD)
The gastrointestinal infectious diseases caused by bacteria have been a major problem in the Thai swine industry. Porcine proliferative enteropathy (PPE), also known as porcine ileitis, is an enteric infectious disease caused by Lawsonia intracellularis. Porcine ileitis has been an economic threat to the global pork production system due to various types of diarrheas. Serological diagnosis can be applied for determining pathogen-antibodies seroprevalence and the timing of L. intracellularis infection when introduced to the herd. A lack of serological data since 2009, as well as recent antibiotic usage restrictions, may have a negative impact on porcine ileitis seroprevalence in Thailand. …
Transferable R Plasmids In Escherichia Coli Isolated From Meat Ducks, Zulqarnain Baqar
Transferable R Plasmids In Escherichia Coli Isolated From Meat Ducks, Zulqarnain Baqar
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to describe the AMR characteristics and transferable R plasmids in Escherichia coli isolated from meat ducks reared in an open house system. One hundred seventy-seven (n=177) commensal E. coli were previously isolated from duck cloacal swabs. In this study, all were biochemically confirmed and examined for their susceptibilities to 15 antimicrobial agents by broth microdilution method. Transfer of R plasmids was tested by biparental mating method followed by plasmid replicon typing (PBRT) and plasmid multi-locus sequence typing (pMLST). The highest resistance rates were observed for ampicillin (83.0%) and tetracycline (81.9%) while multidrug resistance was common (86.4%). Extended-spectrum …
Phenotypic And Molecular Characterization Of Streptococcus Agalactiae Causing Bovine Mastitis In Central Region Of Thailand, Sirirat Wataradee
Phenotypic And Molecular Characterization Of Streptococcus Agalactiae Causing Bovine Mastitis In Central Region Of Thailand, Sirirat Wataradee
Chulalongkorn University Theses and Dissertations (Chula ETD)
Streptococcus agalactiae is a highly contagious pathogen with a significant economic impact on bovine mastitis. The research aims to achieve three objectives: 1) identifying and characterizing S. agalactiae strains responsible for bovine mastitis based on their phenotypic and genotypic characteristics, 2) determining susceptibility profiles to antimicrobial agents, and 3) applying molecular epidemiological analysis to explore intramammary infections in Thai dairy herds. We analyzed a collection of one hundred S. agalactiae isolates from clinical and subclinical mastitis cases in thirteen dairy herds in central region of Thailand. We used conventional microbiological procedures, the VITEK® 2 system, and molecular detection of the …
Influence Of Cataract On Electroretinal Responses In Small Dog Breeds, Chayada Noosen
Influence Of Cataract On Electroretinal Responses In Small Dog Breeds, Chayada Noosen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine cataract is a condition of lens opacity that if severe, can lead to vision loss. High prevalence of cataract is evident in small dog breeds. To assess retinal function in cataract patients, flash electroretinography is commonly used in practice. This study aimed to compare time- and frequency-domain electroretinal responses between normal and cataract eyes in small dog breeds. Eighty-four eyes from small dog breeds categorized into immature cataract (n=49), mature cataract (n=25) and control (n=10) eyes in this study. Full-field flash intensity series ERG was performed in dogs under scotopic and photopic condition. Data was analyzed and compared among …
Porcine Semen Microbiota Analysis In Relation To Sperm Quality In Semen Extender Coupled With Gentamicin, Cong Bang Ngo
Porcine Semen Microbiota Analysis In Relation To Sperm Quality In Semen Extender Coupled With Gentamicin, Cong Bang Ngo
Chulalongkorn University Theses and Dissertations (Chula ETD)
The present study was carried out to (i) investigate the association between boar semen microbiota and semen qualities and (ii) determine the relationship between boar bacteria contamination and longevity of boar semen preserved for 3 days in BTS with and without gentamicin. A total of 20 Duroc ejaculates were collected from standard quality boars (n=10) and high-quality ones (n= 10). Besides 3 ml of fresh semen of each ejaculate was subjected to 16S rRNA gene sequencing and bioinformatics including alpha diversity, beta diversity, and taxonomic mapping, each ejaculate was diluted in two conditions of Beltsvile Thawing Solution (BTS) extender: CONTROL: …
The Effect Of Platelet-Rich Plasma And Hyaluronic Acid Nanogel On Wound Healing In Rat Skin Models, Pirada Virankabutra
The Effect Of Platelet-Rich Plasma And Hyaluronic Acid Nanogel On Wound Healing In Rat Skin Models, Pirada Virankabutra
Chulalongkorn University Theses and Dissertations (Chula ETD)
The study aimed to validate two-step procedure PRP protocol and assess the effect on skin healing process in rats treated with PRP and/or HA nanogel. A total of 46 Wistar rats were enrolled in the study. PRPwas produced and its hematological parameters were examined. Six skin defects were created and topicals (control, PRP, HA, PRP/HA, PRP/HA cream, and cream) were applied to the wounds. The progression of wound reduction was photographed daily, and calcutated to wound area. Skin samples were collected according to sampling timepoints: day 0, 3, 7,10, and 14, and stained for histological grading. The platelet (2457.15 $ …
Correlation Of The Classical Swine Fever (Csf) Antibody Levels Detected By Serum Neutralizaton And Enzyme-Linked Immunosorbent Assay (Elisa), Chairani Ridha Maghfira
Correlation Of The Classical Swine Fever (Csf) Antibody Levels Detected By Serum Neutralizaton And Enzyme-Linked Immunosorbent Assay (Elisa), Chairani Ridha Maghfira
Chulalongkorn University Theses and Dissertations (Chula ETD)
Classical swine fever is an important viral disease that has a devastating impact on the swine industry. The Enzyme-linked immunosorbent assay (ELISA) provides a simpler and more practical approach to detect classical swine fever virus (CSFV) antibodies compared to the serum neutralization (SN) test. However, antibody responses detected by ELISA cannot directly exhibit a protective level. This study aimed to evaluate the correlation of classical swine fever antibody responses detected by SN assay and commercial ELISA. A total of 522 negative and positive serum samples were tested by the SN and ELISA. Correlation, an agreement between two assays, and comparisons …
Establishment Of Transplantation Platform For Delivering Mouse Induced Pluripotent Stem Cell-Derived Insulin-Producing Cells (Mips-Ipcs) For Diabetes Treatment, Hong Thuan Tran
Chulalongkorn University Theses and Dissertations (Chula ETD)
Pancreatic beta-cell replacement is recognized for feasible type 1 diabetes (T1D) treatment. However, in post-transplantation, the autoimmune destruction incidentally attacks the activity and survival of beta-cells are reported in animal and human. To address these concerns, the generation of immortalized, biocompatible beta-cells, and the engraftment platform are insightfully investigated. The stepwise chemical process was used for in vitro Insulin-producing cells (IPCs) production from mouse gingival fibroblast-induced pluripotent stem cells (mGF-iPSCs). The real-time qRT-PCR, glucose stimulation C-peptide/Insulin secretion, immunostaining, and visible cell methods were examined during IPC differentiation. The encapsulated-IPC beads were loaded into subcutaneous pocket space via transplantation platform. Completed …
Comparison On The Effects Of Tiletamine/Zolazepam And Dexmedetomidine With And Without Tramadol Combination Undergoing Calvarial Surgery In Rats., Kaewkarn Manomaya
Comparison On The Effects Of Tiletamine/Zolazepam And Dexmedetomidine With And Without Tramadol Combination Undergoing Calvarial Surgery In Rats., Kaewkarn Manomaya
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aims to evaluate the perioperative anesthetic quality of a dexmedetomidine and tiletamine/zolazepam combination with or without tramadol during rat calvarial surgery. The study employed adult male Wistar rats (Rattus norvegicus), intraperitoneal (IP) administered with a combination of dexmedetomidine (0.1 mg/kg) and tiletamine/zolazepam (25 mg/kg) - referred to as the DZ group, or the addition of tramadol (12.5 mg/kg) - referred to as the DZT group. The procedures have been approved by the Institutional Animal Care Committee, Chulalongkorn University Laboratory Animal Center. The key parameters for anesthetic quality assessment were anesthetic achievement, the onset of induction, and the onset …
Seasonal Dynamics Of Parasitic Vector-Borne Pathogens In Cattle Farms And The Development Of Dna Amplification And Artificial Intelligent-Assisted Detection Methods, Apinya Arnuphapprasert
Seasonal Dynamics Of Parasitic Vector-Borne Pathogens In Cattle Farms And The Development Of Dna Amplification And Artificial Intelligent-Assisted Detection Methods, Apinya Arnuphapprasert
Chulalongkorn University Theses and Dissertations (Chula ETD)
Vector-borne pathogens in cattle have detrimental effects on animal health and the quality of animal products. These pathogens are prevalent worldwide, particularly in tropical and sub-tropical regions like Thailand. Notable vector-borne pathogens affecting cattle in Thailand include Anaplasma marginale, Babesia bovis, B. bigemina, Theileria orientalis, Trypanosoma evansi, and T. theileri. Additionally, climate change influences the behaviour of these vector-borne pathogens through its impact on their vectors. Prompt and accurate diagnosis is crucial to mitigate the adverse effects caused by these pathogens. However, conventional microscopic examination, considered the gold standard, may have limitations in terms of sensitivity and time requirements. Therefore, …
Bacteriophage Nanocarrier As A Crispr-Cas System Delivery Vector For Re-Sensitization Of Blactx-M-M Extended-Spectrum Β-Lactamase-Producing Escherichia Coli From Companion Animals, Naiyaphat Nittayasut
Bacteriophage Nanocarrier As A Crispr-Cas System Delivery Vector For Re-Sensitization Of Blactx-M-M Extended-Spectrum Β-Lactamase-Producing Escherichia Coli From Companion Animals, Naiyaphat Nittayasut
Chulalongkorn University Theses and Dissertations (Chula ETD)
Increased prevalence of blaCTX-M third-generation cephalosporin (3GC)-resistant uropathogenic Escherichia coli (UPEC) in small animals indicates a crisis of an antimicrobial resistance (AMR) situation in veterinary medicine and public health. CRISPR-Cas9 system delivered by bacteriophage is an alternative tool for re-sensitization of AMR bacteria. This study aimed to characterize canine and feline 3GC-resistant UPEC in term of the genetic features and to find predominant blaCTX-M variants to be targets of CRISPR-Cas9 delivered by a bacteriophage nanocarrier in a re-sensitization model. Seven blaCTX-M variants, including group 1 (blaCTX-M-55, -15 and -173) and group 9 (blaCTX-M-14, -27, -65 and -90), were detected in …
Development Of Elisa For Detecting Antibody Against Bartonella Spp. Specific Protein In Cats And Its Application In Natural Infection, Krissda Boonaramrueng
Development Of Elisa For Detecting Antibody Against Bartonella Spp. Specific Protein In Cats And Its Application In Natural Infection, Krissda Boonaramrueng
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aims to evaluate the risk factor and an association between Bartonella spp. infection and immune status by using retroviral-infected cats as an immunocompromised model and to develop the recombinant B. henselae specific antigen protein (17kDa and GroEL)-based ELISA test for antibody detection against Bartonella spp. infection in cats. From 2017 to 2020, 161 client-owned clinical healthy cats at veterinary clinics and hospitals in the Bangkok metropolitan area were recruited and tested for Bartonella spp. infection statuses (PCR and IFA serology), blood profiles, feline retroviral statuses (FIV and FeLV), and T lymphocyte subsets (CD4+, CD8+, and CD4+ to CD8+ …
Efficacies Of Thai Probiotic Strains Against Antibiotic Resistance Bacteria And Modulate Gut Microbiome And Resistome In Pig Model, Prasert Apiwatsiri
Efficacies Of Thai Probiotic Strains Against Antibiotic Resistance Bacteria And Modulate Gut Microbiome And Resistome In Pig Model, Prasert Apiwatsiri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lactic acid bacteria (LAB) have been widely used as probiotics in the livestock industry because of their high potential for antibacterial activity, anticonjugation, antibiofilm capacity, and gut microbiome regulation. Lactobacillus plantarum 22F, 25F (L22F and L25F), and Pediococcus acidilactici 72N (P72N) showed several promising in vitro and in vivo properties, according to our recent research. The objective of this study was to evaluate the Thai LAB efficacy on anticonjugation and antibiofilm activities in E. coli harboring the mcr-1 gene. When compared to the neutralizing cell-free supernatant (CFS), we discovered that the CFS derived from our LAB strains at 1:16 dilution …
In Vitro Generation Of Transplantable Insulin-Producing Cells From Canine Adipose-Derived Mesenchymal Stem Cells, Quynh Le Dang
In Vitro Generation Of Transplantable Insulin-Producing Cells From Canine Adipose-Derived Mesenchymal Stem Cells, Quynh Le Dang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine mesenchymal stem cells (cMSCs) have potential applications for regenerative therapy, including the generation of insulin-producing cells (IPCs) for studying and treating diabetes. In this study, we established a useful protocol for generating IPCs from canine adipose mesenchymal stem cells (cAD-MSCs). Subsequently, in vitro preservation of pluronic F127-coated alginate (ALGPA)-encapsulated cAD-MSC-derived IPCs was performed to verify ready-to-use (RTU) IPCs. IPCs were induced from cAD-MSCs with the modulated three-stepwise protocol. The first step of definitive endoderm (DE) induction showed that the cooperation of Chir99021 and Activin A created the effective production of Sox17-expressed DE cells. The second step for pancreatic endocrine …