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Full-Text Articles in Veterinary Medicine
Contraception In Free-Ranging Long-Tailed Macaques With Special Focus On Anesthesia Management And Development Of Nanobody Targeting Extracellular Domain Of Follicular Stimulating Hormone Receptors, Pakpoom Navanukraw
Chulalongkorn University Theses and Dissertations (Chula ETD)
The overlapping presence of free-ranging long-tailed macaques (Macaca fascicularis) and humans in Thailand and ฆSoutheast Asia resulted in physical violence, ravaging and zoonotic disease transmission. To mitigate these detriments, the synergy of surgical and nonsurgical contraception is essential for imperative population control. This thesis aimed to: 1) enhance animal welfare and safety of macaques undergoing surgical contraception by applying multimodal anesthesia practices; 2) generate alternative non-surgical contraceptives against the extracellular domain (ECD) of follicular stimulating hormone receptors (FSHr) in long-tailed macaques; 3) formulate nano/microstructured microspheres containing anti-FSHr scFv for oral delivery and determine the pharmacokinetics and bioavailabilityIn Study 1, the …
Utilization Of Testosterone-Encapsulated Nanoparticles For Masculinization Of Red Tilapia (Oreochromis Sp.), Jakarwan Yostawonkul
Utilization Of Testosterone-Encapsulated Nanoparticles For Masculinization Of Red Tilapia (Oreochromis Sp.), Jakarwan Yostawonkul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Tilapia (Oreochromis sp.) is a freshwater fish that is highly popular for aquaculture in Thailand. To achieve high yields, good growth rates, and better feed conversion rates, 17-alpha-methyltestosterone are commonly used to induce male sex in farmed tilapia. However, it has been found in previous studies that these synthetic hormones have low water solubility and limited absorption capabilities, leading to excessive hormone use. In this research, lipid-based nanoparticles, a widely used drug delivery technology, were employed to enhance the efficiency of synthetic hormones with low water solubility. This improved the dispersion of hormones in water, enhanced their absorption in the …
Genome Characterization And Development Of Inactivated Vaccine Against Scale Drop Disease Virus (Sddv) In Asian Sea Bass (Lates Calcarifer), Putita Chokmangmeepisarn
Genome Characterization And Development Of Inactivated Vaccine Against Scale Drop Disease Virus (Sddv) In Asian Sea Bass (Lates Calcarifer), Putita Chokmangmeepisarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Scale drop disease virus (SDDV) is a significant pathogen affecting Asian sea bass (Lates calcarifer) in Southeast Asia, causing scale drop syndrome (SDS) and resulting in high mortality and substantial economic losses in aquaculture. Recently, SDDV has been reported in Asian sea bass farms throughout the Asia-Pacific region including Thailand, Indonesia, Malaysia, Singapore, and China. This study employed metagenomic methods to analyze bacterial and viral communities in infected fish tissues from Southeast Asia, leading to the recovery and characterization of the complete genome of SDDV strain TH7_2019. The genome, comprising 131 kb with 134 putative ORFs, showed high amino acid …
Characterization Of Antimicrobial Resistance And Virulence Factors In Multidrug-Resistant Extraintestinal Pathogenic Escherichia Coli Isolated From Canine Urinary Tract, Reproductive Tract And Bloodstream Infections, Parinya Sroithongkham
Chulalongkorn University Theses and Dissertations (Chula ETD)
Despite extensive characterization of uropathogenic Escherichia coli (UPEC) causing urinary tract infections (UTIs), the genetic background of non-urinary extraintestinal pathogenic E. coli (ExPEC) in dogs, such as those causing canine pyometra, prostatic abscesses (PAs), and bloodstream infections (BSIs), remains inadequately understood. This study characterized the virulence determinants of 101 E. coli isolates from canine pyometra (n=61), PAs (n=38), and BSIs (n=2). The analysis revealed a stronger association of pyometra strains with UPEC compared to PA strains. Forty-one isolates were resistant to third-generation cephalosporins and/or fluoroquinolones, with 14 being extended-spectrum β-lactamase producers and carrying blaCTX-M. Whole-genome sequencing was performed on 10 …
Development And Evaluation The Efficacy Of A Water-Soluble Probiotic Microencapsulation Prototype For Pediococcus Acidilactici Isolated From Broiler Chickens, Ratchnida Kamwa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Probiotics are live bacteria that can be given orally to promote gastrointestinal health. A limitation of probiotic use is their diminished efficiency following the loss of live cells during high-temperature processing and storage. Microencapsulation technology has been created to protect probiotic cells, preserve their viability, and maintain their efficacy following manufacture. The aim of this study was to evaluate the effectiveness of a water-soluble microencapsulation method using gum Arabic (GA) and skim milk (SKM) over a three-month storage period following processing. Four strains of Pediococcus acidilactici (BYF26, BYF20, BF9, and BF14) that were typical lactic acid bacteria (LAB) strains isolated …
Novel Vaccination Strategy Against Vibrio Harveyi Infection In Asian Seabass (Lates Calcarifer) Using A Chitosan – Oxygen Nanobubble - Vaccine Complex, Nguyen Giang Thu Lan
Novel Vaccination Strategy Against Vibrio Harveyi Infection In Asian Seabass (Lates Calcarifer) Using A Chitosan – Oxygen Nanobubble - Vaccine Complex, Nguyen Giang Thu Lan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Vibriosis is a bacterial disease caused by Vibrio harveyi (V. harveyi; Vh ) that poses a substantial threat to Asian seabass (Lates calcarifer) aquaculture. In this study, we investigated alternative approaches to conventional injection vaccination for prevention of V. harveyi in Asian seabass. The first approach involved an immersion prime with heat-inactivated V. harveyi vaccine, followed by two oral booster doses (day 14-18 and day 28-32). Immunized fish demonstrated higher expression levels of immune genes (i.e. TNFα, IL1β, CD4, CD8, and IgM) following the immersion prime and the first oral booster dose, peaking at 14 and 28 days post vaccination …
Farrowing Performances And Piglet Characteristics In Highly Prolific Sows In Tropical Environment: Impact Of Aging, Ambient Temperature And Humidity Within The Barn, And Feeds, Yosua Kristian Adi
Farrowing Performances And Piglet Characteristics In Highly Prolific Sows In Tropical Environment: Impact Of Aging, Ambient Temperature And Humidity Within The Barn, And Feeds, Yosua Kristian Adi
Chulalongkorn University Theses and Dissertations (Chula ETD)
Hyperprolific sows have been widely distributed in the global swine industry, including Thailand. Therefore, factors affecting the farrowing performance and piglet characteristics of these sows were investigated to establish effective management practices, especially in tropical climates. Moreover, strategies to enhance sow colostrum production and expedite the farrowing process were also explored. Additionally, deep insight into the effect of aging on farrowing performance, particularly farrowing duration, through investigation of reproductive organ morphology and oxytocin receptor expression on uterine tissue was conducted. The study revealed that sows with a high number of piglets born per litter, a high parity number, and parturition …
Assessment Of Longitudinal Systolic Funcion By Using Tissue Motion Annular Displacement In Hypertrophic Cardiomyopathy Cats, Nadthagarn Glaewketgarn
Assessment Of Longitudinal Systolic Funcion By Using Tissue Motion Annular Displacement In Hypertrophic Cardiomyopathy Cats, Nadthagarn Glaewketgarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
In humans, tissue motion annular displacement (TMAD) providesresponsive and accurate assessment on longitudinal systolic function. The aim ofthis study is to investigate the LV longitudinal systolic function in hypertrophiccardiomyopathy (HCM) and control cats by using TMAD. The study includes 26control cats and 21 HCM cats. Global TMAD were not influence by breed, sex, age,and heart rate. Global TMAD and % Global TMAD significantly decreased in theHCM group (2.86±0.86 mm and 11.46±3.37%) when compared to control group(3.95±0.89 mm and 16.12±2.94%) (p<0.001 and <0.001), suggesting LV longitudinalsystolic dysfunction in HCM cats. LV fractional shortening (LVFS) showed nodifference between the control (52.76±11.63%) and HCM stage group(59.80±13.51%) (p=0.061). Indicate that TMAD is a sensitive technique foridentifying LV longitudinal systolic dysfunction. Global TMAD and % global TMADhad a significant correlation with global longitudinal strain (GLS). Therefore, TMADcan be used to evaluate longitudinal systolic function in HCM. The reliability test ofglobal TMAD and % global TMAD were considered moderate. In conclusion, globalTMAD and % global TMAD was significantly decreased in cats with HCM comparedwith the control group and was considered as sensitive and repeatable techniquefor evaluating LV longitudinal systolic dysfunction in cats with HCM
Investigating Of Candidate Genes Affecting Milk Production In Dairy Cattle Using Systematic Review And Whole Genome Sequencing Database, Xiaoxian Su
Chulalongkorn University Theses and Dissertations (Chula ETD)
Milk production traits in dairy cattle, such as milk yield, fat content, and protein levels, are pivotal to the economic success of the dairy industry. These traits not only determine the quality and quantity of milk produced but also directly impact the profitability of dairy operations. Consequently, a deep understanding of the genetic architecture underlying these traits is essential for the development of effective breeding programs aimed at enhancing milk production and quality. The purpose of this study is to identify potential candidate genes related to milk production in Thai dairy cattle through systematic review of genome wide association study …
Safety Assessment Of Lactic Acid Bacteria (Lab) As Potential Probiotics For Thai Swine And Poultry: A Whole Genome Sequencing Approach, Yuda Disastra
Safety Assessment Of Lactic Acid Bacteria (Lab) As Potential Probiotics For Thai Swine And Poultry: A Whole Genome Sequencing Approach, Yuda Disastra
Chulalongkorn University Theses and Dissertations (Chula ETD)
The standard criteria for determining the safety of bacteria used as probiotics do not exist at this time. The objective of this investigation was to assess the feasibility and safety of eight lactic acid bacteria (LAB) strains employed as probiotics. Of these, four were Pediococcus acidilactici strains (IAF6519, IAF5919, AF2519, and AF2019), one Ligilactobacillus salivarius AF2319 derived from chickens and Pediococcus acidilactici P72N and Lactiplantibacillus plantarum L25F and L22F obtained from pigs. Phenotypic investigation was performed on biochemical features, antimicrobial resistance patterns, and hemolytic activity. P. acidilactici AF2519 and AF2019 strains were resistant to erythromycin and clindamycin, Ligilactobacillus salivarius strain …
Dynamic Community Of Strongyle Nematodes And Ivermectin Resistant Evidence Of Domesticated Horses In Thailand, Mohamed Hossameldein Abdelfatah Mohamed Hamad
Dynamic Community Of Strongyle Nematodes And Ivermectin Resistant Evidence Of Domesticated Horses In Thailand, Mohamed Hossameldein Abdelfatah Mohamed Hamad
Chulalongkorn University Theses and Dissertations (Chula ETD)
Strongylids are a group of parasitic nematodes that infect horses and other equids worldwide, with a potential negative impact on their health and performance. There is no available research that has studied strongylids infection in domesticated horse populations nor assessed the effectiveness of ivermectin in treating these parasites in Thailand. This thesis, therefore, aimed to comprehensively investigate strongyle parasite intensity and factors influencing egg shedding patterns. Additionally, it utilized nemabiome metabarcoding approach to identify strongylid species, analyse ecological diversity, and assess co-occurrence patterns within the studied horse populations. Furthermore, it employed faecal egg count reduction (FECR) test to assess the …
Prioritization Of Candidate Genes For Mastitis Resistance Trait In Genome-Wide Association Studies (Gwas) Utilizing Multiple Methodologies In Thai Dairy Cattle, Nhu Le Uyen Vo
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mastitis affects milk yield in dairy cattle worldwide, leading to economic losses. Recognized as a polygenic trait, mastitis resistance is influenced by genetic, environmental, and physiological factors. This study aimed to enhance the genetic architecture and selection of the candidate for mastitis resistance traits in Thai dairy cattle by several methods. The study consisted of two parts. In Part 1, the study is divided into 1A) collecting genes from a systematic review of published GWAS, 1B) Analyzing the gene list with the Thai dairy cattle whole genome sequencing database, and 1C) Performing pathway enrichment analysis to identify candidate genes. Further …
Reliability Of Cardiac Measurement By Vertebral Heart Score Among Different Thoracic Types Of Dogs, Suphat Soeratanapant
Reliability Of Cardiac Measurement By Vertebral Heart Score Among Different Thoracic Types Of Dogs, Suphat Soeratanapant
Chulalongkorn University Theses and Dissertations (Chula ETD)
Vertebral heart score (VHS) is a radiographic tool that widely use in veterinary clinical field for evaluating the heart sizeby measure a heart long and short axes and neutralize with vertebral length. However, VHSs among canine breeds are varied from thereference value. Thoracic type is one of the factors that affect to VHS because the shape of canine thoracic type is the structuralvariations that affect heart orientation. Subsequently, it affects to VHS value in thoracic radiographs. Computed tomography (CT) is anadvanced diagnostic tool that provide a multiplane image without superimposition effect. Therefore, CT provide more accurate heartorientation and its evaluation …
Impact Of Probiotic Supplementation In Lactating Sows On Immune And Oxidative Stress Biomarkers, Short-Chain Fatty Acids, Piglet Performance, And Fecal Microbiome, Chutikan Srisang
Impact Of Probiotic Supplementation In Lactating Sows On Immune And Oxidative Stress Biomarkers, Short-Chain Fatty Acids, Piglet Performance, And Fecal Microbiome, Chutikan Srisang
Chulalongkorn University Theses and Dissertations (Chula ETD)
This clinical trial aimed to investigate the effects of probiotic supplementation in lactating sows on their serum immunoglobulin levels, milk yield, serum malondialdehyde (MDA), fecal short-chain fatty acid levels, piglet performance, and fecal microbiome. The research was conducted at a commercial swine breeding herd in central Thailand. The study included 109 Canadian Landrace × Yorkshire sows, with parity numbers ranging from 1 to 7. The sows were divided into two groups based on their parity numbers: control (n = 61) and treatment (n = 48). The control group sows received a conventional commercial lactation diet without any antibiotic supplementation, starting …
Contamination Of Antimicrobial-Resistant Escherichia Coli, Salmonella Spp., Enterococcus Faecalis, And Enterococcus Faecium In Commercial Raw Meat-Based Diets For Cats And Dogs, Rusmin Indra
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to investigate the contamination of third-generation cephalosporin (3GC)-resistant Escherichia coli, Salmonella spp., Enterococcus faecalis, and E. faecium in commercial raw meat-based diets (RMBDs) for cats and dogs in Thailand. Fifty RMBD products from ten brands were included for total plate count and bacterial isolation. Antimicrobial susceptibility testing was conducted for the targeted bacteria. Extended-spectrum β-lactamase (ESBL) production and the presence of blaCTX-M and mcr genes were detected. Additionally, E. coli phylogroups and Salmonella serovars were identified. The RMBD samples were contaminated by 3GC-resistant E. coli (80.0%), Salmonella spp. (70.0%), E. faecalis (100%), and E. faecium (84.0%). All …
Analytical Performance Of Progesterone Testing Using Surface Plasmon Field-Enhanced Fluorescence Spectroscopy Technology In Dogs, Pimuk Sungprasit
Analytical Performance Of Progesterone Testing Using Surface Plasmon Field-Enhanced Fluorescence Spectroscopy Technology In Dogs, Pimuk Sungprasit
Chulalongkorn University Theses and Dissertations (Chula ETD)
A new clinical chemistry analyzer using surface plasmon field-enhanced fluorescence spectroscopy (SPFS) has been introduced into veterinary diagnostic laboratories as a point-of-care unit, enabling rapid turnaround times for both diagnosis and follow-up. Consequently, the test results must be accurate, precise, and reliable. This research aims to evaluate the method performance of the analyzer. The evaluation was conducted according to Clinical Laboratory Improvement Amendments (CLIA) guidelines using method validation. The performance evaluation included studies on imprecision (within-day and between-day), method comparison, recovery tests, and linearity for determining progesterone concentration. The method validation results showed good and acceptable precision. Serum spiking concentrations …
Volatile Organic Compound Profiles Identifiled In Vaginal Discharge Of Bitches During Estrous Cycle, Amornthep Preechatangkit
Volatile Organic Compound Profiles Identifiled In Vaginal Discharge Of Bitches During Estrous Cycle, Amornthep Preechatangkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Volatile organic compounds (VOCs) are the metabolites of microorganisms. VOCs are low molecular weight and easily evaporate at room temperature, all odorants are VOC. VOCs are different depending on health status and reproductive status. The objective of this study was to profile the VOCs in bitches during estrous cycle, and to compare VOCs between stages of estrous cycle and between pre-ovulatory, ovulation and post-ovulatory periods. A total of 23 normal, healthy bitches requested for ovulation timing as part of the routine breeding management were used in this study. All animals had a previous history of normal estrous cycle without any …
Molecular Investigation And Genetic Characterization Of Canine Astrovirus And Canine Kobuvirus With Associated Pathology In Domestic Dogs In Vietnam And Thailand, Tin Van Nguyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine astrovirus (CaAstV) and canine kobuvirus (CaKoV) are recently recognized pathogens associated with canine gastrointestinal disease (GID), though their exact role remains poorly understood. In both Vietnam and Thailand, these viruses were detected in dogs with and without diarrhea, particularly high frequency in puppies under 6 months old. Co-infection between CaAstV and CaKoV, as well as with other common canine enteric viruses (canine parvovirus, canine enteric coronavirus, canine distemper virus), were also confirmed. Complete coding sequences (CDS) of 7 CaAstV strains and 9 CaKoV strains from Vietnam, and 2 CaAstV strains and 4 CaKoV strains from Thailand were successfully identified. …
Reproductive Performance Of Gilts And Sows In Gestation Group Housing And Free Farrowing System In Tropical Climate, Natchanon Dumniem
Reproductive Performance Of Gilts And Sows In Gestation Group Housing And Free Farrowing System In Tropical Climate, Natchanon Dumniem
Chulalongkorn University Theses and Dissertations (Chula ETD)
Animal welfare is a rising topic in the swine industry, resulting in the adoption of alternative housing systems. However, the knowledge of management practices to mitigate the deleterious effects of these systems on reproductive performance in tropical environments is limited. This thesis aims to determine the impacts of alternative housing systems on the reproductive performance of sows and gilts, comprising 4 parts. i) To compare reproductive performance among conventional crates (CC), group housing gestation pen with temporary farrowing crate (PC), and group housing gestation pen and free-farrowing pen (PP). On average, the farrowing rate of sows in the CC group …
Developmental Model Of Endometrial Cell Culture For The Study Of Equine Endometrosis, Sawita Santiviparat
Developmental Model Of Endometrial Cell Culture For The Study Of Equine Endometrosis, Sawita Santiviparat
Chulalongkorn University Theses and Dissertations (Chula ETD)
The importance and rationale of this thesis were to address the critical knowledge gap in equine endometrosis by developing a comprehensive 3-dimensional (3D) in vitro model of equine endometrial tissue using advanced technology like 3D bioprinting. Study 1 aimed to develop an in vitro model for studying equine endometrial function by isolating and characterizing equine endometrial epithelial cells (eECs) and endometrial mesenchymal stem cells (eMSCs) using a modified tissue explantation outgrowth technique. Both cell types were successfully cultured in specific medium conditions, maintaining their characteristic features. Notably, eECs retained their function in fetal bovine serum (FBS) and KnockOut™ serum replacement …
Innovation Of Alpha-Mangostin Encapsulated With Nanostructured-Lipid Carrier As Non-Surgical Castration In Cats And Survey Attitude Towards Surgical Sterilization In Thailand, Shanaporn Leelakajornkit
Innovation Of Alpha-Mangostin Encapsulated With Nanostructured-Lipid Carrier As Non-Surgical Castration In Cats And Survey Attitude Towards Surgical Sterilization In Thailand, Shanaporn Leelakajornkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Overpopulation of companion animals, particularly dogs and cats, is a global concern, necessitating effective population control policies. This research investigates the attitudes of veterinarians and pet owners in Thailand regarding dog and cat surgical sterilization and the use of progestins as non-surgical contraception. The study also assesses the effects of intratesticular administration of alpha-mangostin encapsulated in nanostructured lipid carriers (AM-NLC) in male cats, and adverse effects. Furthermore, a novel formulation of AM-NLC for intratesticular administration is also developed. Surveys reveal that decisions on sterilization are influenced by species, sex, COVID-19 impacts, and demographic factors. And anesthesia risks remain a primary …
Genetic Diversity Of Influenza A Viruses In Live Bird Markets And Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa
Genetic Diversity Of Influenza A Viruses In Live Bird Markets And Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Influenza A viruses (IAVs) can infect birds and a wide variety of mammals. Live bird markets (LBMs) and free-grazing ducks (FGDs) are the important reservoirs of influenza A viruses in Thailand. This study aims to gain the overall information of IAVs circulated in LBMs in Bangkok and in FGD flocks in Thailand. IAVs surveillance in LBM in Bangkok was conducted to identified the IAVs in LBM during 2019-2020. The IAV-H9N2 (n=8) and IAV-H9Nx (n=2) were isolated from 1,178 swab samples. The IAVs of the previous CU-EIDAs’ LBM surveillance programs during 2014-2018 were included for overall genetic analysis. Total 6 subtypes …
A Comprehensive Study Of African Horse Sickness Outbreak In The Northeastern Region Of Thailand: Outbreak Investigation, Genetic Characterization, And Longitudinal Serological Monitoring, Napawan Bunpapong
Chulalongkorn University Theses and Dissertations (Chula ETD)
African horse sickness (AHS) results from infection with the African horse sickness virus (AHSV). The AHSV is a non-contagious and belongs to genus Orbivirus, family Reoviridae and primarily by Culicoides midges. AHS caused the devastating infection in equine population, including horses, ponies, European donkeys, and mules. AHS maintains its occurrence in southern African countries, it may possess threatening impact to the naïve regions such as Asia or Southeast Asia. The objectives of this study are 1) To investigate the emerging AHS outbreak in the Northeastern region of Thailand, 2) To characterize emerged AHSV in Thailand and 3) To monitor serological …
Development Of A Chitosan Nano-Immersion Vaccine Against Aeromonas Veronii Infection In Red Tilapia (Oreochromis Sp.), Pimwarang Sukkarun
Development Of A Chitosan Nano-Immersion Vaccine Against Aeromonas Veronii Infection In Red Tilapia (Oreochromis Sp.), Pimwarang Sukkarun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Red tilapia (Oreochromis sp.) is a prominent freshwater fish in Thai aquaculture. It has long been afflicted by severe bacterial diseases, ulcerative syndrome, and hemorrhagic septicemia, primarily caused by Aeromonas veronii. Vaccination is a key strategy for control and prevention. In this study a chitosan nano-immersion vaccine has been developed using a nanoemulsion technique and surface modification method with chitosan polymers. The physicochemical properties, mucoadhesive properties, immune response assays, and vaccine efficacy were investigated. The results showed that the vaccine is spherical-shaped, nanometer-sized, and having monodispersed positive charge. The mucoadhesive characterization, observed by SEM and fluorescence microscope imaging, demonstrated that …
The Expressions Of Mhc Class I And Class Ii In Biopsy Tissues By Immunohistochemistry And The Effect Of Interferon-Gamma (Ifn-Γ) On In Vitro Established Canine Cutaneous Mast Cell Tumors, Sudchaya Bhanpattanakul
The Expressions Of Mhc Class I And Class Ii In Biopsy Tissues By Immunohistochemistry And The Effect Of Interferon-Gamma (Ifn-Γ) On In Vitro Established Canine Cutaneous Mast Cell Tumors, Sudchaya Bhanpattanakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine mast cell tumors (MCT), common skin tumors in dogs, present difficulties in treatment, emphasizing the need for effective therapeutic protocols. The cancer-immunity cycle's complex interactions between cancer and the immune system play a crucial role in tumor growth. Tumors often evade immune response by downregulating MHC expression, hindering T-cell recognition. Cancer immunotherapy is a promising approach that utilizes the patient's immune system for combating cancer. Interferon-gamma (IFN-γ), a potent immune substance, plays a crucial role in enhancing MHC expression and immune functionality, crucial for T-cell recognition and cancer cell elimination. Limited research exists on MHC expression, tumor immune response, …
Upscale Production Of Canine Adipose-Derived Mesenchymal Stem Cell-Derived Exosomes For Wound Healing In Vitro, Yudith Violetta Pamulang
Upscale Production Of Canine Adipose-Derived Mesenchymal Stem Cell-Derived Exosomes For Wound Healing In Vitro, Yudith Violetta Pamulang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mesenchymal stem cell-derived exosomes (MSC-derived exosomes) have been previously reported to enhance wound healing across different phases. However, its practical application has been hindered due to challenges regarding low productivity and the lack of efficient production protocol. Additionally, the usage of exosome-based regenerative treatments in the veterinary field remains relatively unexplored. Hence, this study aimed to address these gaps by establishing a potential upscaling protocol producing canine adipose-derived mesenchymal stem cell-derived exosomes (cAD-MSC-exosomes) and exploring their wound healing potential in vitro. To facilitate production protocol establishment, an in-house exosome-collecting media, designated as VSCBIC-3, was preliminarily developed. Building upon this foundation, …
Therapeutic Efficacy Of Subconjunctival Injectionof Canine Adipose-Derived Mesenchymal Stem Cells On Canine Spontaneous Chronic Corneal Epithelial Defect, Pechchalee Kengkla
Therapeutic Efficacy Of Subconjunctival Injectionof Canine Adipose-Derived Mesenchymal Stem Cells On Canine Spontaneous Chronic Corneal Epithelial Defect, Pechchalee Kengkla
Chulalongkorn University Theses and Dissertations (Chula ETD)
Spontaneous chronic corneal epithelial defects (SCCEDs) are important corneal injuries in dogs. Canine adipose-derived mesenchymal stem cells (cAD-MSCs) was used for treatment of various types of corneal ulcers in dogs. This study aimed to evaluate clinical outcomes and concentrations of tear fluid cytokine after subconjunctival injection of cAD-MSCs in canine SCCEDs. 9 canine SCCEDs eyes previously treated by debridement twice were included into this study. All eyes received a single subconjunctival injection of 1x106 cAD-MSCs. Ophthalmic examinations were undergone at day 7, 14 and 21 after treatment. Photographs of the cornea were taken to assess corneal epithelial defect, neovascularization and …
Nail Cortisol For Welfare Assessment In Shelter And Owned Cats, Minh Pham Quoc Anh
Nail Cortisol For Welfare Assessment In Shelter And Owned Cats, Minh Pham Quoc Anh
Chulalongkorn University Theses and Dissertations (Chula ETD)
Shelter cats are vulnerable to stress due to the challenging circumstances they face, including confinement, unfamiliar environments, people, and animals. This vulnerability often leads to welfare issues, emphasizing the importance of assessing and improving their well-being. Measuring cortisol levels, a stress hormone, from nails has emerged as a non-invasive method to evaluate the stress response in humans and other animals. This study aimed to apply nail cortisol for welfare assessment in cats by measuring and comparing nail cortisol concentrations (NCCs) of owned cats and shelter cats living in different conditions. Nails of 31 owned cats (OC), 29 shelter cats living …
Canine Pd-L1 Mrna And Protein Expression In Normal Tissues And Common Cancers, Fuengfa Damjamnong
Canine Pd-L1 Mrna And Protein Expression In Normal Tissues And Common Cancers, Fuengfa Damjamnong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Programmed cell death-ligand 1 (PD-L1), an immune check points molecule, is expressed differently in various tissues and plays an important role in common cancers. The objectives of this study are to investigate the expression of canine PD-L1 mRNA and protein in normal tissues and common cancers and to determine the correlation of PD-L1 expression with histologic grading of the malignant mammary gland tumor (MGT). In the normal tissue, the highest PD-L1 mRNA expression was observed in lymph node (P<0.01). In the cancer tissues, the highest PD-L1 mRNA expression was observed in lymphoma, but not significant (P=0.224). The PD-L1 mRNA expression was higher in benign tumors compared to malignant MGT (P=0.093) but not significant. Immunohistochemistry (IHC) was performed in formalin fixed paraffin embedded tissue of normal and MGT tissue using newly developed anti-canine PD-L1 monoclonal antibody (mAb) (clone 5D2). Immunostaining was detected on epithelial cells, transitional cell, parenchymal cells, alveolar macrophages and immune cells. The immunostaining score of malignant MGTs was higher than benign MGT (P<0.05). The immunostaining score was not significantly different among histological sub-types and histological grades. The lack of correlation between PD-L1 mRNA and protein expression in canine malignant MGTs was observed. In conclusion, this anti-canine PD-L1 mAb could be used as predictive biomarker for canine MGT. This study has fulfilled and expanded a knowledge of the PD-L1 checkpoint molecule in dogs and highlights a potential of PD-L1 antibodies using for immunotherapy in canine MGTs, similar to their application in human tumors.
Metabolomic Profiles Of Mammary Gland Secretion In Relation To Dam’S Weight, Litter Size, Parity Number, And Kid’S Growth In Black Bengal Goat, Tien Vo Thi Phuong
Metabolomic Profiles Of Mammary Gland Secretion In Relation To Dam’S Weight, Litter Size, Parity Number, And Kid’S Growth In Black Bengal Goat, Tien Vo Thi Phuong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Black Bengal goat, a small size meat-type goat which is known to have high litter size and low milk yield. Kids born from high litter size dams are at high risk of mortality. Colostrum contains high quantities of nutrients, growth factors, and passive antibodies, and is essential for kid survival and development. However, metabolomic profiles of mammary secretion in goats are still limited. The aims of this study were to investigate metabolomic profiles of mammary secretion of purebred Black Bengal goats and their relationships regarding to litter size, parity, dam health, and kid growth. Forty-three colostrum samples (D0) were collected …