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Full-Text Articles in Veterinary Medicine
Prevalence And Molecular Characteristics Of Antimicrobial Resistance Of Aeromonas Hydrophila, Salmonella Spp., Vibrio Cholerae, Fecal Coliform, And Escherichia Coli In Hybrid Red Tilapia And Cultured Water, Varangkana Thaotumpitak
Prevalence And Molecular Characteristics Of Antimicrobial Resistance Of Aeromonas Hydrophila, Salmonella Spp., Vibrio Cholerae, Fecal Coliform, And Escherichia Coli In Hybrid Red Tilapia And Cultured Water, Varangkana Thaotumpitak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Foodborne pathogens and antimicrobial resistant bacteria in tilapia and cultivation water has been emerged as a public health threat. This study aimed to determine the prevalence of indicator and pathogenic bacteria in hybrid red tilapia (Oreochromis spp.) and their cultivation water, and to characterize phenotypic and genotypic AMR, virulence genes, and extended-spectrum beta-lactamase (ESBL) production of Escherichia coli, Aeromonas hydrophila, Salmonella spp., and Vibrio cholerae. A total of 120 tilapia, which were comprised of carcass rinse (n=120), muscle (n=120), intestine (n=120), liver and kidney (n=120), and cultivation water (n=120) were collected from tilapia farms in Kanchanaburi province, Thailand during October …
Identification, Characterization And Pathogenicity Of Aeromonas Schubertii Isolated From Diseased Striped Snakehead Fish (Channa Striata), Hendri Budiyansah
Identification, Characterization And Pathogenicity Of Aeromonas Schubertii Isolated From Diseased Striped Snakehead Fish (Channa Striata), Hendri Budiyansah
Chulalongkorn University Theses and Dissertations (Chula ETD)
Striped snakehead fish (Channa striata) is one of the popular cultivated fish in Thailand. However, it is inevitable to be susceptible to abundant pathogens, especially Aeromonas schubertii. In July 2020, an unknown outbreak was occurred on striped snakehead fish nursery farm in Nakhon Pathom province, Thailand. Suspected six isolates were A. schubertii yielded from the fish as the main causative agent. This research aimed to characterize and examine pathogenicity of A. schubertii isolated from diseased striped snakehead fish. Characterization was performed based on combination of biochemical assay, PCR and DNA sequencing (based on 16S rRNA and gyrB), and MALDI-TOF MS. …
Genetic Diversity And Distribution Of Tilapia Lake Virus In Fish Polyculture System In Bangladesh, Partho Pratim Debnath
Genetic Diversity And Distribution Of Tilapia Lake Virus In Fish Polyculture System In Bangladesh, Partho Pratim Debnath
Chulalongkorn University Theses and Dissertations (Chula ETD)
Tilapia is the world's second most important farmed fish species, following carp. Bangladesh is the world's fourth-largest tilapia producer and has largely adopted fish polyculture. Few research on factors associated with mortality and economic losses following such deaths have been undertaken in Bangladesh due of its perceived hardiness. Tilapia lake virus (TiLV) is an emerging pathogen in aquaculture, reportedly affecting farmed tilapia in 16 countries across multiple continents. Following an early warning in 2017 that TiLV could be widespread, we surveyed 565 tilapia farms in 15 of Bangladesh's most important tilapia-producing districts using online tilapia epidemiology and health economics survey …
The Urinary Proteomic Analysis And The Vitamin D Receptor Polymorphisms In Dogs With Calcium Oxalate Urolithiasis, Sumonwan Chamsuwan
The Urinary Proteomic Analysis And The Vitamin D Receptor Polymorphisms In Dogs With Calcium Oxalate Urolithiasis, Sumonwan Chamsuwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Calcium oxalate (CaOx) urolithiasis is one of the most common stone components which frequently occurs in both humans and dogs. Hypercalciuria is one of the predisposing factors commonly found in both people and dogs with calcium urolithiasis. The genetic factors are also involved with the pathogenesis of stone formation, and the relationship between calcium handling and vitamin D receptor (VDR) polymorphisms has been demonstrated to be related to calcium urolithiasis in human populations. Moreover, some urinary proteins may be involved in the process of stone formation. The present study aimed firstly to evaluate the relationship between VDR polymorphism in dog …
Relationship Between Insulin-Like Growth Factor-1 And Semen Quality In Asian Elephants (Elephas Maximus), Yuqing Yang
Relationship Between Insulin-Like Growth Factor-1 And Semen Quality In Asian Elephants (Elephas Maximus), Yuqing Yang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Evidence has been increasingly suggested that insulin-like growth factor-1 (IGF-1) is related to steroidogenesis and spermatogenesis. This may be conducive to find the causes of poor sperm quality in Asian elephants (Elephas maximus). The present study aimed to investigate whether correlations among serum IGF-1 concentration, serum testosterone level and semen variables exist in elephants. A total of 17 ejaculates (1–3 ejaculates/bull) were collected by performing transrectal massage. Before each ejaculate, blood samples were obtained (n = 17). Subsequently, semen characteristics of each ejaculate were evaluated. Assessments of precision (calculations of the intra- and inter-assay coefficient of variation, CV) and accuracy …
The Effect Of Maternally-Derived Antibodies Against Antibody Production In Vaccinated Piglets With Modified-Live Porcine Reproductive And Respiratory Syndrome Virus Vaccines, Sunit Mebumroong
Chulalongkorn University Theses and Dissertations (Chula ETD)
The humoral antibody responses following vaccination with different lineage of modified live genotype 2 porcine reproductive and respiratory syndrome virus (PRRSV) vaccines (MLVs) were investigated. Moreover, the mortality and growth performance in pigs were observed. Twenty-four pigs with high maternally derived antibody (MDA) levels (SN titer ≥ 8) from each PRRSV positive Herd 1 and Herd 2 were allocated to the H1, H1-1, and H1-2 groups (Herd 1) and H2, H2-1, and H2-2 groups (Herd 2). Twenty-four pigs with low MDA levels (SN titer <8) were allocated to the L1, L1-1, and L1-2 (Herd 1) and L2, L2-1, and L2-2 groups (Herd 2). Twenty-four pigs from the negative herd (Herd 3) were allocated to 3 groups: N, N-1, and N-2 groups. The H1, H2, L1, L2, and N groups were unvaccinated. The H1-1, H2-1, L1-1, L2-1, and N-1 groups were vaccinated with MLV (Ingelvac® PRRS MLV) at 2 weeks of age. The 5 other groups: H1-2, H2-2, L1-2, L2-2, and N-2 groups were vaccinated with MLV2 (Prime Pac® PRRS MLV) at 2 week of age. The antibody responses were measured by ELISA and serum neutralization (SN). The mortality was not observed in all vaccinated groups. Additionally, pigs with high MDA levels from the H1 and H2 groups did not exhibit the mortality throughout the study. ADG in pigs vaccinated with each MLV1 and MLV2 was significant higher than that in unvaccinated pigs. However, pigs vaccinated with MLV2 showed the highest values of ADG. Seroconversion was observed in all vaccinated groups with no significance difference in antibody titers between pigs with low and high MDA levels. However, pigs with low MDA levels showed the earliest and highest antibody level post vaccination. The highest SN-titer against MLV1 isolate and MLV2 isolate was observed in pig from the L1-1 and L2-2 groups, respectively. The field PRRSV-2 isolates: THA_SP/RB_S1/P1/0120-18 and THA_WC/RB_F165/20-22, the SN titers against these isolates were not observed in vaccinated groups. These results indicated that MDAs might not affect the immune response post vaccination. PRRSV-2 MLVs reduced the mortality and improved growth performance of pigs in the endemically PRRSV infected herds. Although, the vaccine was changed in weaning pigs, the antibody responses were detected post vaccination in both pigs with low and high level of MDAs. Moreover, the genetic similarity between vaccine and field virus was not related to the protection. Vaccine selection should depend on the induction of immune response and protection against heterologous PRRSV infection.
A Comparison Of Ketamine Combined With Dexmedetomidine And Ketamine Combined With Xylazine On Analgesia In Rabbits, Aomusa Kuaha
A Comparison Of Ketamine Combined With Dexmedetomidine And Ketamine Combined With Xylazine On Analgesia In Rabbits, Aomusa Kuaha
Chulalongkorn University Theses and Dissertations (Chula ETD)
The effects of ketamine combined with dexmedetomidine and ketamine combined with xylazine on analgesia and cardiopulmonary variables in rabbits were evaluated in eight healthy New Zealand White rabbits. The experiment used a randomized double-crossover design. The rabbits were randomly divided into two groups, Group KD (n=4: 2 males/2 females) receivied 35 mg kg -1of ketamine and 0.5 mg kg -1of dexmedetomidine SC, while Group KX (n=4: 2 males/2 females) receivied 35 mg kg -1of ketamine and 5 mg kg -1of xylazine SC. The physiological parameters included HR, RR, PR, SpO2, SBP and RT were determined every 5 minutes and continuously …
Effects Of Single Bolus Injection Of Pimobendan On Cardiac Function, Hemodynamics, And Heart Rate Variability In Healthy Dogs, Poonavit Pichayapaiboon
Effects Of Single Bolus Injection Of Pimobendan On Cardiac Function, Hemodynamics, And Heart Rate Variability In Healthy Dogs, Poonavit Pichayapaiboon
Chulalongkorn University Theses and Dissertations (Chula ETD)
In veterinary medicine, pimobendan has been recommended for management of dogs with congestive heart failure for decades. Recently, an intravenous injectable solution of this drug has been launched. However, the effects of this preparation on electrocardiography, cardiac functions, hemodynamics, heart rate variability (HRV), and the pharmacokinetics (PK) in dogs are still limited. Therefore, the objective of this investigation is to investigate the hemodynamics, cardiac functions, electrocardiographic parameters, the PK profiles and its active metabolite, O-desmethyl pimobendan (ODMP), as well as its effect on HRV of a single bolus pimobendan in healthy dogs. Nine dogs were anesthetized and instrumented to obtained …
Pharmacokinetics, Pharmacodynamics And Dosing Regimen Of Long-Acting Oxytetracycline In Nile Tilapia (Oreochromis Niloticus), Kananuch Vasuntrarak
Pharmacokinetics, Pharmacodynamics And Dosing Regimen Of Long-Acting Oxytetracycline In Nile Tilapia (Oreochromis Niloticus), Kananuch Vasuntrarak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Antimicrobial resistance has become a serious global problem and is steadily increasing worldwide. In aquaculture, there are limited antimicrobial options for treatment. Thus, there are growing needs for more specific dosing regimens of existing antimicrobial drugs that are not only to obtain therapeutic efficacy but also to minimize the resistance of pathogens. The purposes of this study were to determine pharmacokinetics (PK) of long-acting oxytetracycline (OTC) after intraperitoneal (IP) administration in Nile tilapia. One hundred and twenty healthy male tilapia (450±37.47 g) were divided into two experimental groups (60 fish/group). Each group received OTC-LA single IP injection at dosage of …
Osteogenic Differentiation Potential By Mouse Bone Marrow-Derived Mesenchymal Stemcells (Mbm-Mscs) Derived From Gut Leak-Induced Diabetes Type Ii Mouse Model, Nabila Syarifah Jamilah
Osteogenic Differentiation Potential By Mouse Bone Marrow-Derived Mesenchymal Stemcells (Mbm-Mscs) Derived From Gut Leak-Induced Diabetes Type Ii Mouse Model, Nabila Syarifah Jamilah
Chulalongkorn University Theses and Dissertations (Chula ETD)
Diabetes type II were known to affect several organs in the body including bone, thus diabetes type II patients have higher risk of bone fracture. One of the important features in diabetes type II is low level of anti-inflammatory cytokine Interleukin-10 (IL-10) and gut leak condition. Both of these conditions have the role in diabetes induction, progression and exacerbation. Autogenous Mesenchymal stem cells (MSCs) have been widely utilized for enhancing bone healing treatment. Due to the diabetes condition affecting one of the most common and widely used MSCs source, the bone, this study was done to investigate the effect of …
A Study Of Using Corncob, Aspen Shaving, Dried Water Hyacinth And Banana Midrib As Bedding For Laboratory Mice, Nantaporn Maytayapirom
A Study Of Using Corncob, Aspen Shaving, Dried Water Hyacinth And Banana Midrib As Bedding For Laboratory Mice, Nantaporn Maytayapirom
Chulalongkorn University Theses and Dissertations (Chula ETD)
A study of using corncob, aspen shaving, dried water hyacinth and banana midrib as bedding for laboratory mice. The objective of this study was to evaluate the physical characteristics (i.e., absorbency and surface moisture), the physiological effects and intracage ammonia level of 4 types of beddings in laboratory mice. This study was divided into two parts. In the first part, the fluid absorption properties of these four beddings were studied using measurement of liquid absorption and the surface moisture. The results showed that the volumetric absorbency of aspen shaving was significantly higher than other beddings. While, the surface moisture showed …
Comparison Of Laser Class 1m And Class 4 Applications In Canine Cranial Cruciate Ligament Rupture Evaluated By Clinical Outcomes And Ultrasonography, Noppakrit Ekakaraworapong
Comparison Of Laser Class 1m And Class 4 Applications In Canine Cranial Cruciate Ligament Rupture Evaluated By Clinical Outcomes And Ultrasonography, Noppakrit Ekakaraworapong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Nineteen dogs diagnosed of cranial cruciate ligament rupture were included in this study before TPLO was performed. They were classified into 3 groups which consisted of control, low-level laser therapy class 1M and low-level laser therapy class 4 groups. The number of each group were 4, 8 and 7 dogs, respectively, and the duration of treatment protocol was 4 weeks consecutively. On the first day of the study, dogs were examined and assessed of the clinical signs such as lameness score, synovial fluid analysis, canine brief pain inventory questionnaire (CBPI) and ultrasonographic score. In ultrasonographic monitoring, ultrasonographic scores on synovial …
Bacteriophage And Ozone-Nanobubble Based Approaches For Combating Multidrug-Resistant Aeromonas Hydrophila In Nile Tilapia (Oreochromis Niloticus) Culture System, Thanh Dien Le
Chulalongkorn University Theses and Dissertations (Chula ETD)
Aeromonas hydrophila infection is considered one of the most important bacterial diseases responsible for the loss of millions of dollars in the global freshwater aquaculture industry. Multidrug-resistant (MDR) A. hydrophila is becoming a global issue of concern. Non-antibiotic approaches can minimize the requirement for antimicrobials to combat infectious diseases and antimicrobial resistance. This study aims to investigate the potential application of lytic bacteriophage, ozone nanobubbles (NB-O3), a potential combination of bacteriophage and nanobubble-based technology in combatting MDR A. hydrophila in Nile tilapia. A newly Myoviridae phage pAh6.2TG was isolated, identified, and characterized in this study. The application of this phage …
Effect Of Bubaline Blood-Derived Platelet Rich Fibrin (Prf) In Canine Periodontitis, Poranee Banyatworakul
Effect Of Bubaline Blood-Derived Platelet Rich Fibrin (Prf) In Canine Periodontitis, Poranee Banyatworakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Periodontal disease is one of the most common oral disease in elderly dogs. The effect of periodontal disease is generalized contribute to several systemic conditions. Bioactive surgical additives have been introduced in small animal treatment to reduce inflammation and to enhance periodontal healing. Platelet-rich fibrin (PRF), a clumping of platelets, leucocyte, growth factors and cytokines trapping in a dense fibrin meshworks, is considered as a novel healing biomaterial. Autologous PRF have been demonstrated an excellent response in canine periodontal regeneration. However, a complicated health status should be concerned to obtain a high volume. Xenogenic blood donor is now focusing to …
Genetic And Pathological Investigations Of Viruses In Rabies-Negative Brain Of Cats And Dogs, Sabrina Wahyu Wardhani
Genetic And Pathological Investigations Of Viruses In Rabies-Negative Brain Of Cats And Dogs, Sabrina Wahyu Wardhani
Chulalongkorn University Theses and Dissertations (Chula ETD)
All year round, The Thai Red Cross Society received more than 100 animal samples that were suspected for rabies to be tested. Most of these animals were suspected for rabies due to the clinical signs of neurological disorder they developed before they die. Interestingly, in greater number of cases, rabies infection was ruled out as the cause of neurological disorder by direct fluorescent antibody test (dFAT). In respect to the importance of virus in the outcome of neurological disorder, this study was initiated to investigate the underlying cause of viral infection in the brain of rabies-negative cats and dogs that …
The Study Of Changes In Gene Regulation Of Serotonin Signaling Pathway And Circulating Serotonin Concentration In Dogs With Pulmonary Hypertension Due To Degenerative Mitral Valve Disease, Nattawan Tangmahakul
The Study Of Changes In Gene Regulation Of Serotonin Signaling Pathway And Circulating Serotonin Concentration In Dogs With Pulmonary Hypertension Due To Degenerative Mitral Valve Disease, Nattawan Tangmahakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Pulmonary hypertension (PH) is an abnormal increase in pulmonary vascular pressure. In dogs, PH is commonly found secondary to degenerative mitral valve disease (DMVD), especially in small breed dogs. Serotonin, a biogenic amine playing an essential role in both physiology and abnormalities of several organs, is a vasoactive substance that has been one of the suspicious mediators for the development of PH. Both local and circulating effects of serotonin have been investigated to discover the involvement of serotonin and the pathogenesis of PH in both humans and animals. The present study aimed to investigate the local serotonin signaling in lung …
Genetic Characteristics Of Antimicrobial Resistance Of Salmonella Enterica And Escherichia Coli Isolated From Broilers, Pigs And Their Meat Products In Sa Kaeo, Banteay Meanchey And Siem Reap Provinces, Suthathip Trongjit
Chulalongkorn University Theses and Dissertations (Chula ETD)
This research study aimed to investigate the incidence and characterize antimicrobial resistance (AMR) in Salmonella enterica and Escherichia coli isolated from broilers, pigs and their meat products in Thailand–Cambodia border provinces. Resistance to clinically importance antimicrobials was additionally examined in the E. coli isolates from other provinces with high pig production in Thailand. A total of 941 samples were collected from broilers and pigs at slaughterhouses and their carcasses at local fresh markets in Sa Kaeo, Thailand (n=554) and Banteay Meanchey and Siem Reap, Cambodia (n=387) during 2014-2015. Three hundred forty-five Salmonella isolates were collected from Thailand (n = 145; …
Pharmacokinetics And Antibacterial Activity Of Fosfomycin Against Escherichia Coli From Urinary Tract Infected Dogs, Nattha Jariyapamornkoon
Pharmacokinetics And Antibacterial Activity Of Fosfomycin Against Escherichia Coli From Urinary Tract Infected Dogs, Nattha Jariyapamornkoon
Chulalongkorn University Theses and Dissertations (Chula ETD)
Using old or uncommon drugs as alternative choices are interesting option to avoid drug resistance problems. Fosfomycin is an old antibacterial drug with broad spectrum and excreted via urine in active form. Fosfomycin tromethamine (FOS) has been approved to treat uncomplicated urinary tract infection (UTI) in women by FDA. However, in dogs, FOS data is inadequate and precise dosages have not been established yet. Thus, this study aims to determine pharmacokinetics (PK) and pharmacodynamics (PD) of FOS in E. coli from dog with UTI. A total of 6 healthy male beagle dogs were administered with FOS in 3 different dosages …
Recovery Of Isolated Pancreatic Isletsderived From Gut Leak-Induced Diabetes Type Ii Mouse Model, Adretta Soedarmanto
Recovery Of Isolated Pancreatic Isletsderived From Gut Leak-Induced Diabetes Type Ii Mouse Model, Adretta Soedarmanto
Chulalongkorn University Theses and Dissertations (Chula ETD)
Gut leak in the obese patient was known to be one of the predisposing factors causing diabetes type II. Indeed, the interleukin-10 (IL-10) plays a key role in gut mucosa homeostasis preventing the gut leak. Nevertheless, the linkage of diabetes type 2 caused by gut leak with IL-10 deficiency in the non-diabetes patient has not been investigated yet. Islets transplantation is known as the best alternative treatment for diabetes. The culture of isolated islets is still facing difficulties to preserve the morphology, viability, and functionality. Therefore, the present study was aiming to prove the concept of gut-leak on IL-10 deficient …
Effect Of Dietary Microencapsulated Organic Acid And Essential Oil On Sow Performance And Nursery Piglet Growth Performance And Fecal Bacteria, Aprilia Rizky Riadini
Effect Of Dietary Microencapsulated Organic Acid And Essential Oil On Sow Performance And Nursery Piglet Growth Performance And Fecal Bacteria, Aprilia Rizky Riadini
Chulalongkorn University Theses and Dissertations (Chula ETD)
The effect of feed supplementation with microencapsulated organic acids and essential oils (MOE, Porcinate+) in feed was investigated according to sow and nursery pig growth performances, and fecal bacteria population. Experiment 1: healthy three hundred and twenty sows during its late gestation period (7 days before farrowing) until the weaning phase (28 days after farrowing) were randomly divided as control group (160 sows) which was fed with basal diet, and treatment group (160 sows), which was fed with basal diets supplemented with 2 kilograms (kg) of MOE in 1 ton of feed. Each of 4 replications was composed of 40 …
Porcine Reproductive And Respiratory Syndrome Virus: Genetic Diversity, Pathogenesis And Modified-Live Vaccines, Adthakorn Madapong
Porcine Reproductive And Respiratory Syndrome Virus: Genetic Diversity, Pathogenesis And Modified-Live Vaccines, Adthakorn Madapong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine reproductive and respiratory syndrome virus (PRRSV) is a causative agent of PRRS that reproductive failure in sows and respiratory problems in piglets is the hallmark of the disease. Recently, several PRRSV modified live vaccines (MLV) are available, and vaccine selection is a concern. Therefore, the objectives of this study were: 1) to investigate the genetic diversity of Thai PRRSV isolates during 2001-2017; 2) to evaluate the pathogenicity of Thai PRRSV isolates; 3) to determine the efficacy of PRRSV MLV against Thai field PRRSV infection and 4) to investigate the effectiveness of PRRSV MLV when administered via intramuscular (IM) and …
Clinical Diagnosis, Ultrastructural Study And Antigenic Characterization Of Platynosomum Fastosum In Cats, Babi Kyi Soe
Clinical Diagnosis, Ultrastructural Study And Antigenic Characterization Of Platynosomum Fastosum In Cats, Babi Kyi Soe
Chulalongkorn University Theses and Dissertations (Chula ETD)
Platynosomum fastosum is a cat liver fluke causing hepatobiliary diseases in cats. To evaluate the criterior to support the suspicion index for early disease detection during subclinical infection, the biochemical parameters composed of liver, pancreatic enzymes, tumor markers were evaluated in 14 P. fastosum naturally-infected cats. As a result, alanine transaminase (ALT) and gamma glutamyl transferase (GGT) were found elevated in 57.8% (8/14) of infected cats. For the diagnostic imaging, bile sediments were present in 85.7% (12/14) of infected cats in their gallbladders. Since the number of bile-derived P. fastosum eggs were significantly higher than that from fecal samples (p=0.001), …
Genetic Characteristics Of Antimicrobial Resistance In Escherichia Coli Isolated From Pigs, Pork And Humans In Thailand And Lao Pdr Border Provinces, Chanika Pungpian
Genetic Characteristics Of Antimicrobial Resistance In Escherichia Coli Isolated From Pigs, Pork And Humans In Thailand And Lao Pdr Border Provinces, Chanika Pungpian
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this study were to monitor the prevalence of antimicrobial resistance (AMR) among Escherichia coli isolated from pigs, pork and humans in Thailand-Laos border areas, characterize AMR of E. coli isolated from pigs, pork and humans in Thailand-Laos border areas, and compare the resistance to last-line antimicrobials of E. coli and Salmonella isolated from pigs and pork in Thailand-Laos and Thai-Cambodia border areas. Three study projects were conducted. Project 1 demonstrated the results of phenotypic and genotypic monitoring of AMR in E. coli in pigs, pork, and humans in Thailand and Lao PDR border provinces. A total of …
Fabrication Of Three-Dimensional (3-D) Corneal Transplantable Model From Silk Fibroin/Gelatin-Based Biomaterial, Chutirat Torsahakul
Fabrication Of Three-Dimensional (3-D) Corneal Transplantable Model From Silk Fibroin/Gelatin-Based Biomaterial, Chutirat Torsahakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine corneal blindness is a common cause of vision loss and visual impairment worldwide. While the demand of canine corneal graft is continuously elevated, the clinical useable grafts are limited to acellular biomaterials and allograft. This study aimed to generate the tissue-engineered canine cornea in the part of corneal epithelium and underlying stroma based on cLESCs (canine limbal epithelial stem cells) seeded silk fibroin and gelatin (SF/G) film and cCSSCs (canine corneal stromal stem cells) seeded SF/G scaffold respectively. Both cell types were successfully isolated by collagenase I. SF/G films and scaffolds served as the prospective substrates for cLESCs and …
Microbiological Quality Of And Antimicrobial Resistance In Commercial Probiotic Products For Food Animals In Thailand, Hoang My Tran
Microbiological Quality Of And Antimicrobial Resistance In Commercial Probiotic Products For Food Animals In Thailand, Hoang My Tran
Chulalongkorn University Theses and Dissertations (Chula ETD)
A total of 45 commercial probiotic products for food animals were investigated for the number of viable cells, bacterial species and the presence of antimicrobial resistance (AMR) genes. All products were enumerated for viable bacterial cells of Lactobacillus, Bacillus and Enterococcus. Confirmation of species was carried out by multiplex Polymerase Chain Reaction (PCR) for Lactobacillus (n=20), Enterococcus (n=20), and Amplified Ribosomal DNA Restriction Analysis (ARDRA) for Bacillus (n=190). The presence of Clostridium species was examined by PCR. The contamination of E. coli and Salmonella was also determined. Minimal Inhibitory Concentrations (MICs) for 14 antimicrobials was examined in the bacterial isolates …
Detection Of Indicator And Pathogenic Bacteria In Raw Vegetables And Fruits Sold In Fresh Markets And Supermarkets, Bangkok, Thailand, Jutanat Srisamran
Detection Of Indicator And Pathogenic Bacteria In Raw Vegetables And Fruits Sold In Fresh Markets And Supermarkets, Bangkok, Thailand, Jutanat Srisamran
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to compare the concentrations of fecal coliforms and Escherichia coli, and the contamination of Salmonella, Shigella, E. coli O157: H7 and Listeria monocytogenes in raw vegetables and fruits between fresh markets and supermarkets in Bangkok, Thailand. A total of 405 produce samples, consisting of sweet basil, spring onion, coriander, cabbage, lettuce, cucumber and tomato, were randomly collected from seven fresh markets (n=203) and 29 supermarkets (n=202) in seven districts of Bangkok between June 2018 and July 2019. T-test and one-way ANOVA were used to compare the concentrations of fecal coliforms and E. coli (log10MPN/g) between fresh markets …
Targeted Surveillance Of Potential Zoonotic Respiratory And Enteric Viruses In Dogs In Bangkok, Thailand, Kamonpan Charoenkul
Targeted Surveillance Of Potential Zoonotic Respiratory And Enteric Viruses In Dogs In Bangkok, Thailand, Kamonpan Charoenkul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Human-dog interface poses a risk of transmission and spread of zoonotic viruses. The objective of the thesis entitled is to survey and genetic characterize zoonotic viruses in dogs and humans who have high-risk occupations and in close contact with dogs. This thesis contains 8 topics. The results of the first to third topics were the surveillance of respiratory viruses in dogs including Canine Parainfluenza type 5 (CPIV-5), Canine influenza virus (CIV) and Coronavirus (CoV). The nasal swab samples were collected from dogs with respiratory signs during 2015-2018. The results showed that the occurrence of CPIV-5, CIV and CoV were 5.6% …
Comparison Of Canine Urinary Bacterial Population In Calcium Oxalate Urolithiasis And Healthy Conditions, Nichamon Rakprakobkij
Comparison Of Canine Urinary Bacterial Population In Calcium Oxalate Urolithiasis And Healthy Conditions, Nichamon Rakprakobkij
Chulalongkorn University Theses and Dissertations (Chula ETD)
Urolithiasis is one of the most common lower urinary tract diseases in dogs. Struvite is the most canine uroliths reported, and the second reported is calcium oxalate (CaOx). However, the proportion of CaOx urolithiasis tends to be increased. CaOx urolithiasis has high incidence of recurrence after the treatment and the pathogenesis of the stone formation is uncleared. Recent studies revealed that oxalate degrader bacteria in the gastrointestinal tract has important roles in CaOx stone formation but no research has been done to study the composition and the role of microbiome in the urinary tract of dogs with CaOx urolithiasis. This …
Efficiency And Clinical Outcomes Of Platlet-Rich Plasma Therapy On Caninecoxofemoral Osteoarthritis, Patama Arunrattanakul
Efficiency And Clinical Outcomes Of Platlet-Rich Plasma Therapy On Caninecoxofemoral Osteoarthritis, Patama Arunrattanakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
The purpose of this study was to find out for the optimal technique to prepare plasma with highest platelet concentration in platelet rich plasma (PRP), as well as determine the result of autologous PRP from the dogs with osteoarthritis(OA) condition. It was shown that the PRP could reduce inflammation, cell regeneration, and enhance angiogenesis to support the injured tissue. This study was composed of two phases; the first phase was aimed to find the best centrifugation speed and time that could be able to produce the plasma with the highest platelet concentration by conducting the experiment from a total of …
Surveillance Of Coronaviruses In Fruit Bats (Pteropus Lylei) Of Thailand, Pichamon Moolthep
Surveillance Of Coronaviruses In Fruit Bats (Pteropus Lylei) Of Thailand, Pichamon Moolthep
Chulalongkorn University Theses and Dissertations (Chula ETD)
Coronavirus (CoV) is an enveloped single positive-stranded RNA virus CoV can cause enteric and respiratory diseases in mammals, avian and human. Bats are the natural reservoir of CoVs and distribute all over Thailand. Sample collection was performed in two bat colonies in Ayutthaya and Saraburi provinces during March 2018 - February 2019. Total 1,487 samples were collected from 730 bats. The bat species identification via morphological measurement and cytochrome B detection showed that bats in both colonies are Lyle's flying fox (Pteropus lylei). The results of CoV detection showed that 4.30% of samples were positive to CoV. Phylogenetic analysis showed …