Medicine and Health Sciences Commons^™

In Silico And In Vitro Studies Of Taiwan Chingguan Yihau (Nricm101) On Tnf-Α/Il-1Β-Induced Human Lung Cells, Yih-Dih Cheng, Chi‑Cheng Lu, Yuan-Man Hsu, Fuu-Jen Tsai, Da-Tian Bau, Shih-Chang Tsai, Ching-Chang Cheng, Jen-Jyh Lin, Yuang-Yu Huang, Yu-Ning Juan, Yu-Jen Chiu, Sheng-Chu Kuo, Jai-Sing Yang, Lii-Tzu Wu

BioMedicine

COVID-19 pandemic has been a global outbreak of coronavirus (SARS-CoV-2 virus) since 2019. Taiwan Chingguan Yihau (NRICM101) is the first traditional Chinese medicine (TCM) classic herbal formula and is widely used for COVID-19 patients in Taiwan and more than 50 nations. This study is to investigate in silico target fishing for the components of NRICM101 and to explore whether NRICM101 inhibits cytokines-induced normal human lung cell injury in vitro. Our results showed that network prediction of NRICM101 by a high throughput target screening platform showed that NRICM101 has multiple functions that may affect cytokine regulation to prevent human lung cell …

Ethanol Leaf Extract Of Hoslundia Opposita In In Vivo Antioxidant And Hepatoprotective Activity Using An Animal Model, Fred Coolborn Akharaiyi, Odoligie Imarhiagbe, Lucky Efe Isunu, Adebayo Thomas Ajibola

BioMedicine

Introduction and aim: The liver is a useful organ that is responsible for the detoxification of harmful substances from the body. Its vital role is of importance hence it should be protected from damages in order to function normally. The management of liver diseases with orthodox medicine has been found to have side effects thus its effective management with folklore has been found better.

Materials and Methods: The leaves of H. oppisota harvested from a forest was processed and extracted with ethanol. The extract concentrations of 100 to 400 mg/ml was used to evaluate acute toxicity, biochemical analysis, …

The Evaluation Of Serum Lipid Profile And Apolipoprotein C-1 In The Iranian Patients Of Oral Squamous Cell Carcinoma, Walaa Alazzawi, Zahra Shahsavari, Hanieh Babaei, Hadis Firouzpour, Abbas Karimi, Afsaneh Goudarzi

BioMedicine

Background: The key role of apolipoprotein C-1 (ApoC-1) is reported in breast, pancreas and lung cancer. However, no information is available on potential difference of ApoC-1 between OSCC patients and healthy individuals. This work aimed to examine the serum ApoC-1 level as well as lipid profile values between OSCC patients and healthy control groups.

Material and Methods: In this study, 44 blood samples from 22 OSCC patients and 22 healthy individuals were collected to determine the values of lipid profile and ApoC-1 concentration using colorimetric method and Enzyme-Linked Immunosorbent Assay (ELISA), respectively.

Results: A significant decrease in serum lipid profile …

Lep G2548a Polymorphism Is Associated With Increased Serum Leptin And Insulin Resistance Among T2dm Malaysian Patients, Layth Ahmed Ali, Khairunadwa Jemon, Nurriza Ab Latif, Suhaili Abu Bakar, Sharifah Sakinah Syed Alwi

BioMedicine

Background: Type 2 diabetes mellitus (T2DM) is a chronic metabolic syndrome that is rapidly increasing across the world, especially in Malaysia. Leptin plays a vital role in the regulation of metabolism through its effect on peripheral tissues. G2548A polymorphism in the LEP gene promoter has been associated with insulin resistance, leptin, and type 2 diabetes mellitus across different population, but has not been inclusively reported within the Malaysian population.

Objective: Thus, our study aimed to investigate the impact of G2548A polymorphism on serum leptin levels and insulin resistance among Malaysian T2DM patients.

Methods: This case-control study involved …

Neovasculogenic Effect Of 11,12-Epoxyeicosatrienoic Acid Involves The Akt/Enos Signaling Pathways In Human Endothelial Progenitor Cells, Hung-Chang Hung, Jia-Ning Syu, Che-Yi Chao, Shu-Ming Huang, Cheng-Chieh Lin, Mei-Due Yang, Shu-Yao Tsai, Feng-Yao Tang

BioMedicine

The 11,12-epoxy-eicosatrienoic acid (11,12-EET) is formed from arachidonic acid (AA) by cytochrome P450 2J2 (CYP 2J2) epoxygenase and function as an effector in blood vessels. Human endothelial progenitor cells (hEPCs), a preceding cell source for endothelial cells (ECs), involve in the vascular tissue repairing by postnatal neovasculogenesis. However, the effect of 11, 12-EET on hEPCs and neovasculogenesis is not well known. In the current study, we examined the function of 11, 12-EET in hEPCs-mediated neovasculogenesis by using tubular formation analysis, Western Blotting assay, immunofluorescence staining, flow cytometry analysis and zymogram analysis. The results suggest that 11, 12-EET significantly induces neovasculogenesis …

Phenotype And Genotype In A Taiwanese Girl With Sotos Syndrome, Wei-De Lin, Chung-Hsing Wang, Fuu-Jen Tsai, I-Ching Chou

BioMedicine

Rare copy number variations have been linked to an important source of mutation in many psychopathological traits and neurodevelopmental disorders. In this study, we describe a Taiwanese girl with mental retardation and mild macrocephaly who underwent a childhood psychological evaluation for several years first. When she was 5 years old, she came to our hospital for further diagnosis. We conducted molecular cytogenetic tests and confirmed she actually has Sotos syndrome. We compared our case with two others with very similar deletion regions, but their phenotypes were heterogeneous. Sotos syndrome is very rare in Taiwan, and it is suggested that genetic …

Covid19 Vaccines And The Misinterpretation Of Perceived Side Effects, Raymond Palmer

BioMedicine

In the era of Covid19 and mass vaccination programs, the anti-vaccination movement across the world is currently at an all-time high. Much of this anti-vaccination sentiment could be attributed to the alleged side effects that are perpetuated across social media from anti-vaccination groups. Fear mongering and misinformation being peddled by people with no scientific training to terrorise people into staying unvaccinated is not just causing people to remain susceptible to viral outbreaks, but could also be causing more side effects seen in the vaccination process. This brief review will offer data that may demonstrate that misinformation perpetuated by the anti-vaccination …

Characteristics, Emotional Experience, And Impact Of Unplanned Out-Of-Hospital Births In The City Of Beit Shemesh, Israel, Allison Rosenbaum, Dm, Cnm, Mss

Midwifery Thinks! Symposium

Allison Rosenbaum, CNM, MSS, DM has been living and practicing midwifery in Israel since 2002. She obtained a master’s degree in social work from the Bryn Mawr Graduate School of Social Work and Social Research in 1994, then went on to study nursing and midwifery at MGH School of Health Professionals and Philadelphia University’s Institute of Midwifery and Women’s Health (now Thomas Jefferson University), earning her Master of Science in 2018 and her Doctorate in Midwifery in 2022. Currently, she works at Assuta Medical Center in Ashdod, Israel, and is the director of their waterbirth program. She is also involved …

Evaluation Of Classical Guidance Programs During The Covid-19 Pandemic At Junior High School: Discrepancy Model, Hani Nurrofifah, Muh. Farozin, Asfarina Mutiara

Jurnal Kajian Bimbingan dan Konseling

Guidance and counseling programs are designed to improve education. These programs continue to evolve, especially the classical guidance programs that require adaptation to the current Covid-19 pandemic education system. However, due to a lack of planning, the development and implementation of the classical guidance program in state junior high schools during the Covid-19 pandemic have not been optimal. Thus, this study identifies the performance of the classical guidance program at state junior high schools during the Covid-19 pandemic, using predetermined standards. This study used program evaluation standards and criteria from the Guidelines for Performance-Based Professional School Counselor Evaluation. The guidelines …

Exploring The Association Of Physician Characteristics To Patient Requests For Genetic Testing, Vanessa B. Crowther, Sandra G. Suther, Jullet Davis Weaver, Clement K. Gwede, Matthew T. Dutton, Dongming Cui, Ivette A. López

Florida Public Health Review

Background: Cancer genomic testing improves health outcomes for individuals at risk, drives cost-efficiency, and facilitates healthcare equity; however, little is known about how physician demographic and practice characteristics influence patient requests for genetic testing. Purpose: To explore whether (and to what extent) physician demographic and practice characteristics are associated with patient requests for cancer genetic testing. Methods: A cross-sectional quantitative design survey was distributed to 1240 primary care physicians registered with the state health department who had active licenses and main practices in Florida. Primary care physicians were defined as those who practice family medicine, internal medicine, obstetrics, and gynecology. …

Occupational Health And Safety Trainees Reflect On Their Education And Future Careers During Covid-19., Jennifer Marshall Phd, Mph, Cph, Vidya Chandran Mba, Mph, Cph, Bds, Davies Toluhi Mbchb, Mph, Cph, Karen D. Liller Phd, Mph, Cph, Saloni Mehra Mph, Cph, Candace Burns Phd, Ms, Aprn, Faaohn

Florida Public Health Review

Background: The National Institute for Occupational Safety and Health (NIOSH) funded Education and Research Centers (ERCs), located at 18 universities, with the mission to train occupational safety and health (OSH) leaders. The Florida Sunshine ERC has trained hundreds of students since its inception in 1997 through seven programs that collaboratively foster interdisciplinary education and applied research and practice. The COVID-19 pandemic has presented practical challenges for educators, students, and trainees, forcing institutions to move to remote learning. The pandemic also magnifies the importance of public health and OSH.

Purpose: This evaluation elicited feedback from ERC trainees early in the …

Covid-19 Vaccination: Public Health Lessons From A Large Indoor Gathering, Nidhi Manikkoth, Kirthika Venkatesan, Subrahmanya Bhat, Madhavan V Pillai, Nisha Nigil Haroon

Florida Public Health Review

COVID-19 transmission rates among vaccinated persons attending large gatherings have not been reported widely. This research was intended to track the potential incidence of COVID-19 among physicians and their families who attended a large in-person gathering in Atlanta in August 2021. After the successful conclusion of a large-scale indoor gathering, we encouraged all attendees to self-report the incidence of COVID-19 illness. In addition, an online questionnaire was disseminated to collect basic information about age, gender, place of residence, vaccination status including the number of doses, type, and date of each dose as well as behavioral and convention factors that would …

A Comparison Of Diet And Physical Activity Behaviors Of High School Students In Duval County And Nationally: 2019 Yrbs, Bethley Luctama, Sydney Bridges, Kaitlyn Kol, Jack Sparkman, Judith Rodriguez

Florida Public Health Review

Background: Establishing proper physical activity and dietary behaviors during adolescence is crucial because these habits will carry on with the individual into adulthood. Proper physical activity and nutrition reduce the risk of illness and ailments.

Purpose: Identifying physical activity and dietary behaviors of adolescents in Duval County, FL, and the U.S. nationally. This study examines the differences between the Duval County and national samples using gender and grade comparisons.

Methods: The study utilized data from the 2019 Youth Behavior Risk Survey for high school students. Analysis was conducted for eight questions encompassing both physical activity and dietary behaviors to examine …

Comparison Of Major Adverse Cardiovascular Events Risk With Androgen Deprivation Therapy By Personal History Of Cardiovascular Diseases Using Real World Data, Jason Hafron, Deborah Boldt-Houle, Stuart Atkinson

Website/Multimedia Contributions

No abstract provided.

Professional Identity In Guidance And Counseling: The Perspective Of Prospective Counselors, Fathur Rahman, Yuli Nurmalasari, Siti Aminah, Agus Taufiq

Jurnal Kajian Bimbingan dan Konseling

This study aims to describe prospective counselors’ perceptions of professional identity concepts in the guidance and counseling field. This study used a qualitative approach with an exploratory, descriptive design. Research data were collected through open questionnaires and focus group discussions. The data sources were fifteen guidance and counseling students from three different classes. Data analysis was carried out by searching for themes and determining the correlation between them to attain interpretation. The results of the study show that professional identity is conceptualized as a typical characteristic or a symbol of professional conduct in the profession of guidance and counseling. Meanwhile, …

Healthy Exosomes And Their Effects On Diabetic Cardiomyocytes, Miguel A. Garza, Genaro A. Ramírez-Correa, Maria Lourdes Garza-Rodríguez, Andres J. Medina

MEDI 9331 Scholarly Activities Clinical Years

Extracellular Vesicles, and more specifically, exosomes, are essential for effective cell-to-cell communication in a wide variety of tissues. In the last couple of decades, these nanovesicles have been proven to be active participants and regulators in many disease processes; therefore, their therapeutic effects have been widely studied and proven in various cardiovascular diseases both, in vitro and in vivo. Thus, this study aims at assessing the effects of running healthy mice exosomes on cardiomyocyte and cardiac tissue samples obtained from diabetic mice. Here, we successfully extract exosomes from mice plasma and detect their presence through the use of anti-CD9 and …

Development Of Siap Antisipasi (Sianti) To Enhance Students’ Assertive Behavior In Preventing Physical Sexual Harassment On Junior High School Students, Fitri Ratnasari, Elia Flurentin, Arbin Janu Setiyowati

Jurnal Kajian Bimbingan dan Konseling

This research and development aims to develop a product called Siap Antisipasi (SIANTI) to improve assertive behavior in preventing physical sexual harassment in junior high school students by meeting product acceptance. SIANTI’s products include three educational videos with a video duration of three minutes each and a guidebook for school counselors in providing classical guidance services. The research method by adapting Borg and Gall model. This research and development procedure includes preliminary studies, product design and development as well as product testing of material experts, media experts and users. The research and development subjects consisted of one material expert, one …

Effectiveness Of Solution-Focused Brief Counseling And Motivational Interviewing In Improving Students’ Self-Disclosure, Iftitah Indriani, Mungin Eddy Wibowo, Mulawarman Mulawarman

Jurnal Kajian Bimbingan dan Konseling

This study investigates the effectiveness of solution-focused brief counseling and motivational interviewing in enhancing students’ self-disclosure. This study used a randomized pretest-posttest control group design. The sixteen participants from State Junior High School 3 Comal, Indonesia, were selected through nonprobability sampling with purposive sampling. The participants were divided into experiment and control groups. For the research instruments, this study used The Self-Disclosure Questionnaire developed by Jourard to measure students’ self-disclosure. The obtained data were analyzed using the repeated measures ANOVA test. Our analysis results showed that solution-focused brief counseling and motivational interviewing were effective in improving students’ self-disclosure. Therefore, the …

Addressing Public Speaking Anxiety In Multicultural Counselling: The Use Of Cognitive-Behavioural Therapy For International Students, Moh. Khoerul Anwar, Muhammad Izzul Haq, Diafi Salma, Zhongjun Wang

Jurnal Kajian Bimbingan dan Konseling

Several studies have highlighted the importance of Cognitive Behavioural Therapy (CBT) in reducing public speaking anxiety. The studies also denote the increase of confidence by strengthening self-efficacy. This study explores previous studies that uncover the use of CBT in dealing with public anxiety to identify its potential as part of multicultural counseling for international students in higher education. International students have different academic and learning cultures, as well as social environments, affecting the development of certain anxiety in dealing with pressures to adapt to new environments. Therefore, it positions the investigation of CBT’s ability to reduce public speaking anxiety for …

Make A Wish: Happy Birthday Bladder Candle, Alexander Geisenhoff, Mit Shah, Megan Schober

Website/Multimedia Contributions

No abstract provided.

Successful Treatment Of Symptomatic Cystitis Glandularis Presenting As Bladder Mass, With Cox-2 Inhibitor: A Case Report, Bilal Muhammad, Kenneth Peters

Website/Multimedia Contributions

No abstract provided.

Isopsa: Clinical Performance Of A Single Parameter, Structure Based Test For High Grade Prostate Cancer Unaffected By 5-Ari And A-Blocker Medications, Eric Klein, Alan Partin, Yair Lotan, Jack Baniel, Martin Dineen, Jason Hafron, Kannan Manickam, Marc Pliskin, Matthew Wagnet, Ao Zhang

Website/Multimedia Contributions

No abstract provided.

Capg Is Required For Ebola Virus Infection By Controlling Virus Egress From Infected Cells, Hiroyuki Mori, James P Connell, Callie J Donahue, Ruthmabel Boytz, Yen Thi Kim Nguyen, Daisy W Leung, Douglas J Lacount, Robert A Davey

2020-Current year OA Pubs

The replication of Ebola virus (EBOV) is dependent upon actin functionality, especially at cell entry through macropinocytosis and at release of virus from cells. Previously, major actin-regulatory factors involved in actin nucleation, such as Rac1 and Arp2/3, were shown important in both steps. However, downstream of nucleation, many other cell factors are needed to control actin dynamics. How these regulate EBOV infection remains largely unclear. Here, we identified the actin-regulating protein, CAPG, as important for EBOV replication. Notably, knockdown of CAPG specifically inhibited viral infectivity and yield of infectious particles. Cell-based mechanistic analysis revealed a requirement of CAPG for virus …

Apoptosis Detection In Retinal Ganglion Cells Using Quantitative Changes In Multichannel Fluorescence Colocalization, Xudong Qiu, Seth T Gammon, James R Johnson, Federica Pisaneschi, Steven W Millward, Edward M Barnett, David Piwnica-Worms

2020-Current year OA Pubs

KcapTR488 is a dual-fluorophore peptide sensor for the real-time reporting of programmed cell death by fluorescence imaging. KcapTR488 contains a nuclear localization sequence (NLS) conjugated with Texas Red, a caspase-cleavable sequence (DEVD), and a C-terminus conjugated to Alexa Fluor 488 (AF488). The synthesis and preliminary evaluation in cellulo of KcapTR488 for monitoring cell death by fluorescence imaging has been previously reported, but its utility in vivo has yet to be tested or validated. Herein, in vitro solution experiments verified the intramolecular fluorescence resonance energy transfer (FRET) between the two fluorophores and enabled a quantitative analysis of enzyme rates and selectivity. …

Weight Stability In Adults With Obesity Initiating Medical Marijuana Treatment For Other Medical Conditions, Michelle R. Lent, Meghan Visek, Paulina Syracuse, Karen L Dugosh, David Festinger

PCOM Scholarly Works

Few studies have evaluated weight change in patients who initiate medical marijuana treatment to address diagnosed health concerns. The objective of this study was to examine whether patients initiating medical marijuana use for a qualifying health condition experienced changes in health and biopsychosocial functioning over time, including weight gain or loss. Specifically, this observational, longitudinal study evaluated changes in the body mass index (BMI) of adults with co-morbid obesity (body mass index [BMI] ≥ 30 kg/m²) and severe obesity (BMI ≥ 40 kg/m2) who were starting medical marijuana treatment for any of the 23 qualifying medical conditions at …

Assessing Age-Specific Vaccination Strategies And Post-Vaccination Reopening Policies For Covid-19 Control Using Seir Modeling Approach, Xia Wang, Hulin Wu, Sanyi Tang

Faculty, Staff and Student Publications

As the availability of COVID-19 vaccines, it is badly needed to develop vaccination guidelines to prioritize the vaccination delivery in order to effectively stop COVID-19 epidemic and minimize the loss. We evaluated the effect of age-specific vaccination strategies on the number of infections and deaths using an SEIR model, considering the age structure and social contact patterns for different age groups for each of different countries. In general, the vaccination priority should be given to those younger people who are active in social contacts to minimize the number of infections, while the vaccination priority should be given to the elderly …

Examining The Association Between Rurality And Positive Childhood Experiences Among A National Sample, Elizabeth L. Crouch Ph.D., Janice C. Probst Ph.D., Sylvia Shi, Alexander C. Mclain Ph.D., Jan M. Eberth Ph.D., Monique J. Brown Ph.D., Melinda A. Merrell, Kevin J. Bennett

Faculty Publications

Purpose

The present study examines the association between rurality and positive childhood experiences (PCEs) among children and adolescents across all 50 states and the District of Columbia. Recent work has quantified the prevalence of PCEs at the national level, but these studies have been based on public use data files, which lack rurality information for 19 states.

Methods

Data for this cross-sectional analysis were drawn from 2016 to 2018 National Survey of Children's Health (NSCH), using the full data set with restricted geographic data (n = 63,000). Descriptive statistics and bivariate analyses were used to calculate proportions and unadjusted associations. …

Inpatient Physical Activity Across A Large University City Hospital: A Behavioral Mapping Study, Karin Valkenet, Prue Mcrae, Elja Reijneveld, Marielle Jans, Petra Bor, Lotte Van Delft, Daniel L. Young, Cindy Veenhof

Physical Therapy Faculty Research

Background: Physical inactivity is common during hospitalization. Physical activity has been described in different inpatient populations but never across a hospital. Purpose: To describe inpatient movement behavior and associated factors throughout a single university hospital. Methods: A prospective observational study was performed. Patients admitted to clinical wards were included. Behavioral mapping was undertaken for each participant between 9AM and 4PM. The location, physical activity, daily activity, and company of participants were described. Barriers to physical activity were examined using linear regression analyses. Results: In total, 345 participants from 19 different wards were included. The mean (SD) age was 61 (16) …

Somatic Variants In Diverse Genes Leads To A Spectrum Of Focal Cortical Malformations, Dulcie Lai, Meethila Gade, Edward Yang, Hyun Yong Koh, Jinfeng Lu, Nicole M Walley, Anne F Buckley, Tristan T Sands, Cigdem I Akman, Mohamad A Mikati, Guy M Mckhann, James E Goldman, Peter Canoll, Allyson L Alexander, Kristen L Park, Gretchen K Von Allmen, Olga Rodziyevska, Meenakshi B Bhattacharjee, Hart G W Lidov, Hannes Vogel, Gerald A Grant, Brenda E Porter, Annapurna H Poduri, Peter B Crino, Erin L Heinzen

Faculty, Staff and Student Publications

Post-zygotically acquired genetic variants, or somatic variants, that arise during cortical development have emerged as important causes of focal epilepsies, particularly those due to malformations of cortical development. Pathogenic somatic variants have been identified in many genes within the PI3K-AKT-mTOR-signalling pathway in individuals with hemimegalencephaly and focal cortical dysplasia (type II), and more recently in SLC35A2 in individuals with focal cortical dysplasia (type I) or non-dysplastic epileptic cortex. Given the expanding role of somatic variants across different brain malformations, we sought to delineate the landscape of somatic variants in a large cohort of patients who underwent epilepsy surgery with hemimegalencephaly …

Phenotypic And Genetic Spectrum Of Atp6v1a Encephalopathy: A Disorder Of Lysosomal Homeostasis, Renzo Guerrini, Davide Mei, Katalin Kerti-Szigeti, Sara Pepe, Mary Kay Koenig, Gretchen Von Allmen, Megan T Cho, Kimberly Mcdonald, Janice Baker, Vikas Bhambhani, Zöe Powis, Lance Rodan, Rima Nabbout, Giulia Barcia, Jill A Rosenfeld, Carlos A Bacino, Cyril Mignot, Lillian H Power, Catharine J Harris, Dragan Marjanovic, Rikke S Møller, Trine B Hammer, Riikka Keski Filppula, Päivi Vieira, Clara Hildebrandt, Stephanie Sacharow, Luca Maragliano, Fabio Benfenati, Katherine Lachlan, Andreas Benneche, Florence Petit, Jean Madeleine De Sainte Agathe, Barbara Hallinan, Yue Si, Ingrid M Wentzensen, Fanggeng Zou, Vinodh Narayanan, Naomichi Matsumoto, Alessandra Boncristiano, Giancarlo La Marca, Mitsuhiro Kato, Kristin Anderson, Carmen Barba, Luisa Sturiale, Domenico Garozzo, Roberto Bei, Laura Masuelli, Valerio Conti, Gaia Novarino, Anna Fassio

Faculty, Staff and Student Publications

Vacuolar-type H+-ATPase (V-ATPase) is a multimeric complex present in a variety of cellular membranes that acts as an ATP-dependent proton pump and plays a key role in pH homeostasis and intracellular signalling pathways. In humans, 22 autosomal genes encode for a redundant set of subunits allowing the composition of diverse V-ATPase complexes with specific properties and expression. Sixteen subunits have been linked to human disease. Here we describe 26 patients harbouring 20 distinct pathogenic de novo missense ATP6V1A variants, mainly clustering within the ATP synthase α/β family-nucleotide-binding domain. At a mean age of 7 years (extremes: 6 weeks, youngest deceased …