Medicine and Health Sciences Commons^™

Effect Of Impression Techniques On Soft Tissue Vertical Displacement In Removable Partial Denture. A Pilot Study, Christine Hanna, Nada El Osta, Jihad Fakhouri

International Arab Journal of Dentistry

Abstract Purpose: The purpose of this study was to evaluate the vertical displacement of soft tissue during different impression procedures using various spacing of the custom tray, and to determine which impression technique would allow the minimal movement. Material and Methods: 21 partially edentulous subjects were selected and enrolled in the study. Each one of them was subjected to four different impression procedures: three alginate impressions with different spacing of the custom tray and one composed with different impression materials, then casts were constructed. Light-curing acrylic occlusal platform was constructed on the primary model and perforated in 10 different points. …

Urban Health: A Practical Application For Clinical Based Learning, Cynthera Mcneill, Umeika Stephens, Tara Walker

Open Textbooks

Urban Health: A Practical Application for Clinical Based Learning is an openly licensed, peer-reviewed textbook for clinical-based nursing educators covering barriers in urban health and their impact on patient health outcomes. The authors explore perspectives of urban communities, urban patients, and urban healthcare providers to offer insight into how healthcare providers can address disparities in urban healthcare, provide meaningful care with the lived experiences of urban patients in mind, and improve patient-provider communication by moving towards a more solution-driven, team-based care approach. Features include learning activities, exemplars, and case studies.

The Antimicrobial Resistance Crisis Needs Action Now, Nonia Pariente

Research on Antimicrobial Resistance

Antimicrobial resistance is a global problem of increasing proportions that we cannot afford to look away from. This World Antimicrobial Awareness Week, we shine a light on the crisis and ways we can all help to address it.

Methylone And Mdma Pharmacokinetics Following Controlled Administration In Humans, Lourdes Poyatos, Alfredo Fabrizio Lo Faro, Diletta Berardinelli, Giorgia Sprega, Sara Malaca, Simona Pichini, Marilyn A. Huestis, Esther Papaseit, Clara Pérez-Mañá, Francesco Paolo Busardò, Magí Farré

Institute of Emerging Health Professions Faculty Papers

The aim of this study is to define, for the first time, human methylone and HMMC plasma pharmacokinetics following controlled administration of 50–200 mg methylone to 12 male volunteers. A new LC-MS/MS method was validated to quantify methylone, MDMA, and their metabolites in plasma. The study was a randomized, cross-over, double-blinded and placebo-controlled study, with a total of 468 plasma samples collected. First, 10 µL of MDMA-d5, MDA-d5 and methylone-d3 internal standards were added to 100 µL of plasma. Two mL of chloroform and ethyl acetate 9:1 (v/v) were then added, mixed well and centrifuged. The supernatant was fortified with …

Single Nuclei Analyses Reveal Transcriptional Profiles And Marker Genes For Diverse Supraspinal Populations, Zachary Beine, Zimei Wang, Pantelis Tsoulfas, Murray G. Blackmore

Biomedical Sciences Faculty Research and Publications

The mammalian brain contains numerous neurons distributed across forebrain, midbrain, and hindbrain that project axons to the lower spinal cord and work in concert to control movement and achieve homeostasis. Extensive work has mapped the anatomic location of supraspinal cell types and continues to establish specific physiological functions. The patterns of gene expression that typify and distinguish these disparate populations, however, are mostly unknown. Here, using adult mice of mixed sex, we combined retrograde labeling of supraspinal cell nuclei with fluorescence-activated nuclei sorting and single-nuclei RNA sequencing analyses to transcriptionally profile neurons that project axons from the brain to lumbar …

Spatially Aware Dimension Reduction For Spatial Transcriptomics, Lulu Shang, Xiang Zhou

Faculty, Staff and Student Publications

Spatial transcriptomics are a collection of genomic technologies that have enabled transcriptomic profiling on tissues with spatial localization information. Analyzing spatial transcriptomic data is computationally challenging, as the data collected from various spatial transcriptomic technologies are often noisy and display substantial spatial correlation across tissue locations. Here, we develop a spatially-aware dimension reduction method, SpatialPCA, that can extract a low dimensional representation of the spatial transcriptomics data with biological signal and preserved spatial correlation structure, thus unlocking many existing computational tools previously developed in single-cell RNAseq studies for tailored analysis of spatial transcriptomics. We illustrate the benefits of SpatialPCA for …

Effects Of Substance Use Disorder On Oxidative And Antioxidative Stress Markers: A Systematic Review And Meta-Analysis, Thiago Wendt Viola, Rodrigo Orso, Luisa Fossati Florian, Miguel Gomes Garcia, Marco Giovanni Signor Gomes, Eduarda Mascarenhas Mardini, João Paulo Ottolia Niederauer, Aline Zaparte, Rodrigo Grassi-Oliveira

School of Medicine Faculty Publications

Recently, it has been suggested that central and peripheral toxicities identified in persons with substance use disorder (SUD) could be partially associated with an imbalance in reactive oxygen species and antioxidant defenses. We conducted a systematic review and meta-analysis to investigate whether SUD is associated with oxidative stress and to identify biomarkers possibly more affected by this condition. We have included studies that analysed oxidant and antioxidant markers in individuals with SUD caused by stimulants, alcohol, nicotine, opioids, and others (cannabis, inhalants, and polysubstance use). Our analysis showed that persons with SUD show higher oxidant markers and lower antioxidant markers …

Transradial-Transfistula Access For Cardiac Catheterization In Patients With Abandoned Hemodialysis Fistulas, Noah Q. Haroian, Michael P. Savage, David L. Fischman

Department of Medicine Faculty Papers

Guidelines consider radial access a relative contraindication in patients with end-stage renal disease as part of a vessel preservation strategy. Radial access distal to a hemodialysis fistula, what we term transradial-transfistula access, offers a solution to radially access this population without affecting their vessel preservation plan. (Level of Difficulty: Advanced.)

Practical Mouse Model To Investigate Therapeutics For Staphylococcus Aureus Contaminated Surgical Mesh Implants, Madison M. Collins, Brent Race, Ronald J. Messer, Chase Baune, Scott D. Kobayashi, Dan Long, Katie Williams, Aaron M. Hasenkrug, Kim Hasenkrug, Natalia Malachowa

School of Medicine Faculty Publications

Introduction: The use of prosthetic mesh in hernia repair provides a powerful tool to increase repair longevity, decrease recurrence rates, and facilitate complex abdominal wall reconstruction. Overall infection rates with mesh are low, but for those affected there is high morbidity and economic cost. The availability of a practicable small animal model would be advantageous for the preclinical testing of prophylactics, therapeutics, and new biomaterials. To this end, we have developed a novel mouse model for implantation of methicillin-resistant Staphylococcus aureus–infected surgical mesh and provide results from antibiotic and immunotherapeutic testing. Materials and Methods: Implantation of surgical mesh between fascial …

Association Between The Health Belief Model, Exercise, And Nutrition Behaviors During The Covid-19 Pandemic, Keagan Kiely, Bill Mase, Andrew R. Hansen, Jessica S. Schwind

Biostatistics, Epidemiology & Environmental Health Sciences: Faculty Publications

Introduction: The COVID-19 pandemic has affected our nation’s health further than the infection it causes. Physical activity levels and dietary intake have suffered while individuals grapple with the changes in behavior to reduce viral transmission. With unique nuances regarding the access to physical activity and nutrition during the pandemic, the constructs of Health Belief Model (HBM) may present themselves differently in nutrition and exercise behaviors compared to precautions implemented to reduce viral transmission studied in previous research. The purpose of this study was to investigate the extent of exercise and nutritional behavior change during the COVID-19 pandemic and explain the …

Epigenetic Regulation In Chromium-, Nickel- And Cadmium-Induced Carcinogenesis, Lei Zhao, Ranakul Islam, Yifang Wang, Xiujuan Zhang, Ling-Zhi Liu

Department of Medical Oncology Faculty Papers

Environmental and occupational exposure to heavy metals, such as hexavalent chromium, nickel, and cadmium, are major health concerns worldwide. Some heavy metals are well-documented human carcinogens. Multiple mechanisms, including DNA damage, dysregulated gene expression, and aberrant cancer-related signaling, have been shown to contribute to metal-induced carcinogenesis. However, the molecular mechanisms accounting for heavy metal-induced carcinogenesis and angiogenesis are still not fully understood. In recent years, an increasing number of studies have indicated that in addition to genotoxicity and genetic mutations, epigenetic mechanisms play critical roles in metal-induced cancers. Epigenetics refers to the reversible modification of genomes without changing DNA sequences; …

Tonsillectomy In Children With 22q11.2 Deletion Syndrome., Jill M. Arganbright, Paul Bryan Hankey, Meghan Tracy, Srivats Narayanan, Janelle R. Noel-Macdonnell Phd, David G. Ingram Md

Manuscripts, Articles, Book Chapters and Other Papers

Tonsillectomy is one of the most common procedures performed in children, however there are currently no published studies evaluating tonsillectomy in children with 22q11.2 deletion syndrome (22q11DS). With this study, our goal was to investigate the indications, efficacy, and complications of tonsillectomy in a pediatric cohort of patients with 22q11DS. This is a retrospective chart review of patients in our 22q Center's repository. Inclusion criteria were a diagnosis of 22q11DS and a history of tonsillectomy or adenotonsillectomy. Data collected included: indications for tonsillectomy, preoperative and postoperative polysomnography (PSG) results, and surgical complications. In total, 33 patients were included. Most common …

Cortex-Wide Response Mode Of Vip-Expressing Inhibitory Neurons By Reward And Punishment, Zoltán Szadai, Hyun-Jae Pi, Quentin Chevy, Katalin Ócsai, Dinu F Albeanu, Balázs Chiovini, Gergely Szalay, Gergely Katona, Adam Kepecs, Balázs Rózsa

2020-Current year OA Pubs

Neocortex is classically divided into distinct areas, each specializing in different function, but all could benefit from reinforcement feedback to inform and update local processing. Yet it remains elusive how global signals like reward and punishment are represented in local cortical computations. Previously, we identified a cortical neuron type, vasoactive intestinal polypeptide (VIP)-expressing interneurons, in auditory cortex that is recruited by behavioral reinforcers and mediates disinhibitory control by inhibiting other inhibitory neurons. As the same disinhibitory cortical circuit is present virtually throughout cortex, we wondered whether VIP neurons are likewise recruited by reinforcers throughout cortex. We monitored VIP neural activity …

Muscle-Specific Ablation Of Glucose Transporter 1 (Glut1) Does Not Impair Basal Or Overload-Stimulated Skeletal Muscle Glucose Uptake, Shawna L Mcmillin, Parker L Evans, William M Taylor, Luke A Weyrauch, Tyler J Sermersheim, Steven S Welc, Monique R Heitmeier, Richard C Hresko, Paul W Hruz, Francoise Koumanov, Geoffrey D Holman, E Dale Abel, Carol A Witczak

2020-Current year OA Pubs

Glucose transporter 1 (GLUT1) is believed to solely mediate basal (insulin-independent) glucose uptake in skeletal muscle; yet recent work has demonstrated that mechanical overload, a model of resistance exercise training, increases muscle GLUT1 levels. The primary objective of this study was to determine if GLUT1 is necessary for basal or overload-stimulated muscle glucose uptake. Muscle-specific GLUT1 knockout (mGLUT1KO) mice were generated and examined for changes in body weight, body composition, metabolism, systemic glucose regulation, muscle glucose transporters, and muscle [

Multilevel Correlates Of Domain-Specific Physical Activity Among Rural Adults - A Cross-Sectional Study, Alan M Beck, Natalicio H Serrano, Audrey Toler, Ross C Brownson

2020-Current year OA Pubs

BACKGROUND: Increasing physical activity (PA) in rural communities is a vital prevention tactic in multiple chronic diseases; however, little is known on the multilevel correlates of PA rural areas. A better understanding of domain-specific PA adds context for promoting PA in rural communities. The current study sought to determine factors associated with domain-specific and overall moderate-vigorous physical activity (MVPA) in rural communities.

METHODS: Surveys were conducted across 14 rural mid-Western communities, with the final analytical sample including 1241 adults (ages 19-96, M = 57.0 [SD = 16.7], 67.8% female, 83.8% white). Generalized linear models with negative binomial distributions examined the …

Qualitative Analysis Of A Twitter-Disseminated Survey Reveals New Patient Perspectives On The Impact Of Urinary Tract Infection, Marissa Valentine-King, Lindsey Laytner, Casey Hines-Munson, Kiara Olmeda, Barbara Trautner, Sheryl Justice, Christina Ching, Larissa Grigoryan

Faculty, Staff and Students Publications

Few studies have harnessed social media to explore patients’ experiences with urinary tract infection (UTI); therefore, we captured UTI experiences and future research suggestions through a Twitter-disseminated survey. The survey posed three qualitative questions inquiring about the impact of UTIs, greatest UTI management hurdle, and research suggestions. We also asked participants to rate how seriously others perceive UTIs and the importance of UTIs in their life (scale: 1–100 (highest)). The study period spanned from January to June 2021. Coding was performed in duplicate, followed by thematic analysis. Of 466 participants from 22 countries, 128 considered their UTIs recurrent (n …

Temporal Regulation Of Notch Activation Improves Arteriovenous Fistula Maturation, Qunying Guo, Guang Chen, Hunter Cheng, Ying Qing, Luan Truong, Quan Ma, Yun Wang, Jizhong Cheng

Faculty, Staff and Students Publications

Background: Arteriovenous fistula (AVF) maturation is a process involving remodeling of venous arm of the AVFs. It is a challenge to balance adaptive AVF remodeling and neointima formation. In this study we temporally controlled Notch activation to promote AVF maturation while avoiding neointima formation.

Methods: Temporal Notch activation was controlled by regulating the expression of Notch transcription factor, RBP-Jκ, or dnMAML1 (dominant negative MAML2) in vascular smooth muscle cells (VSMCs). AVF mouse model was created and VSMC phenotype dynamic changes during AVF remodeling were determined.

Results: Activated Notch was found in the nuclei of neointimal VSMCs in AVFs from uremic …

An Assessment Of 50 State Early Hearing Detection And Intervention Websites: Is Needed Information Being Provided For Parent Decision Making?, Nichole Westin, Donna Sorkin

Journal of Early Hearing Detection and Intervention

Growth of the Internet as an information resource has provided expanded opportunities for families to easily gather information on a range of topics, including health related topics. State Early Hearing Detection and Intervention (EHDI) websites and other electronic data sources are an important opportunity to support families seeking information on options for their children who have been identified as deaf or hard of hearing. A review was undertaken between August and September 2021 of the 51 US EHDI (state and DC) websites and related information (such as links to others’ sites) to determine if information that is specifcally discussed in …

“It’S About Walking Alongside A Family”: Practitioner Perspectives On Caregiver Coaching With Families Of Children Who Are Deaf Or Hard Of Hearing, Dorie Noll, Sheila Moodie, Ian D. Graham, Beth Potter, Elizabeth M. Fitzpatrick

Journal of Early Hearing Detection and Intervention

Practitioners utilize caregiver coaching in early intervention services, but coaching principles and practices are not well understood in the context of listening and spoken language (LSL) services with families of children who are deaf or hard of hearing. The purpose of this study was to examine practitioners’ experiences with coaching, including definitions, training, and practices they utilize in their work with families. Using semi-structured, qualitative interviews and video observation discussions, this study examined the perspectives of 14 practitioners providing LSL services to families at three intervention sites in the US and Canada. Results indicate that practitioners’ underlying beliefs about their …

Application Of Intervention Mapping To Address Lost To Intervention In Early Hearing Detection And Intervention, Torri Ann Woodruff-Gautherin, Kathleen Cienkowski

Journal of Early Hearing Detection and Intervention

Abstract

Purpose: Detail the application of intervention mapping as a protocol for developing a novel way to address lost to intervention within the early hearing detection and intervention systems.

Design: Intervention mapping (IM) is an approach to behavior change that is typically utilized in public health initiatives. This six-step process walks health program developers, researchers, and policy makers through a rigorous research and community-based approach to understanding why a health concern or problem is present in a community. While using IM to address lost to intervention in Early Hearing Detection and Intervention programs, the focus is on why families, after …

A Systematic Review Of The Effects Of Lena-Based Feedback On Parent-Child Language Interactions In Families With Young Children, Beula M. Magimairaj, Naveen K. Nagaraj, Ana Caballero, Karen F. Munoz, Karl R. White

Journal of Early Hearing Detection and Intervention

Enhancing parent language interactions with children beginning in infancy is important because it results in better language abilities, social skills, and academic outcomes in children. A number of researchers have suggested that parent language interactions with children could be enhanced by giving parents feedback about their language interactions using the Language Environment Analysis (LENA) system. The LENA system records communication exchanges between a child and the adult caregiver and provides an automated analysis of adult word count, child vocalization count, and conversational turn count. We did a systematic review of the studies that investigated the use of LENA-based feedback to …

Nurses’ Perceptions Of The Universal Newborn Hearing Screening Program, Aaron Michael Roman, Michelle O'Connor Kensey

Journal of Early Hearing Detection and Intervention

This study explores the knowledge and perceptions of the newborn hearing screening (NBHS) program from the perspective of nurses and nursing support staff. Registered nurses and nursing support staff (n = 84) completed a digitally administered survey that queried their understanding of JCIH guidelines, perceptions around NBHS administration, and parental anxiety. The results demonstrated that while most respondents felt comfortable and confident about NBHS administration, there was a significant difference in their understanding of appropriate screening milestones compared to JCIH recommendations. Participants further demonstrated an interest in additional learning opportunities related to the NBHS.

Impact Of Social Determinants Of Health On Early Hearing Detection And Intervention Screening/Diagnosis Outcomes, Nannette Nicholson, Ellen A. Rhoades, Rachel E. Glade, Laura Smith-Olinde

Journal of Early Hearing Detection and Intervention

Purpose

We reviewed how Social Determinants of Health relate to health inequities and disparities for Early Hearing Detection and Intervention (EHDI) programs. Then, we examine links between specific sociodemographic factors (maternal age, maternal education, ‘race’/ethnicity) and hearing screening and diagnostic audiology follow-up for newborns in the U.S. and its territories.

Methods

Maternal demographic, hearing screening and diagnostic data extracted from publicly available Centers for Disease Control and Prevention (CDC) records were reported to CDC by personnel responsible for EHDI programs. Data were subjected to statistical analysis using analysis of variance and multiple regression techniques.

Results

Results showed no significant differences …

Assessing Impact Of Covid-19 Pandemic On Receipt And Timeliness Of Newborn Hearing Screening And Diagnostic Services Among Infants Born In Four States, Suhana Ema, Sana Charania, Xidong Deng, Charles Rose, Craig A. Mason, Marcus Gaffney, Brandt Culpepper, Michael Lo, Tri Tran, Marcia Fort

Journal of Early Hearing Detection and Intervention

The study compares receipt and timeliness of newborn hearing screening and follow-up diagnostic services between the pre-pandemic birth cohort and the pandemic birth cohort in four participating states. Findings from this study will help inform state Early Hearing Detection and Intervention (EHDI) programs in the future should major public health event occur again.

Likely Impact Of The Covid-19 Pandemic On Newborn Hearing Screening And Follow-Up Services In The United States In 2020, Kelly Dundon, Suhana Alam, Xidong Deng, Mia Morrison, Treeby Brown, Karl R. White, Linda Hazard, Macia Fort, Kirsten R. Coverstone, Craig Mason, Marcus Gaffney

Journal of Early Hearing Detection and Intervention

This perspective aims to highlight aspects of the Early Hearing Detection and Intervention (EHDI) newborn hearing screening and follow-up processes that were impacted by the COVID-19 pandemic and considers factors that likely impacted follow-up after newborn hearing screening among infants born in the United States during 2020. Efforts to minimize the potential impact of missed or delayed identification of hearing loss in infants and young children will also be discussed to help guide future program improvement activities.

Small-Molecule Protac Mediates Targeted Protein Degradation To Treat Stat3-Dependent Epithelial Cancer, Jinmei Jin, Yaping Wu, Zeng Zhao, Ye Wu

Faculty and Student Publications

The aberrant activation of STAT3 is associated with the etiology and progression in a variety of malignant epithelial-derived tumors, including head and neck squamous cell carcinoma (HNSCC) and colorectal cancer (CRC). Due to the lack of an enzymatic catalytic site or a ligand-binding pocket, there are no small-molecule inhibitors directly targeting STAT3 that have been approved for clinical translation. Emerging proteolysis targeting chimeric (PROTAC) technology-based approach represents a potential strategy to overcome the limitations of conventional inhibitors and inhibit activation of STAT3 and downstream genes. In this study, the heterobifunctional small-molecule-based PROTACs are successfully prepared from toosendanin (TSN), with 1 …

2022-11-22_Michiganoverview_Covidcounts, Michigan Department Of Health & Human Services

Michigan COVID-19 Confirmed Cases by Date of Onset Data

Data was downloaded as an Excel spreadsheet which appeared in the Public Use Datasets section under the Cumulative Data link of Michigan's Coronavirus webpage, under the url https://www.michigan.gov/coronavirus/stats.

Cases are aggregated by the date of onset of COVID-19 symptoms, if known, otherwise by laboratory specimen date if known, otherwise by case referral date. Deaths are aggregated by the date of death if known, otherwise by the last date of a change to the case status.

2022-11-22_Michiganoverview_Isdreporting, Michigan Department Of Health & Human Services

Michigan COVID-19 Outbreak Reports

The K-12 Aged ISD Reporting page was obtained from Michigan's COVID-19 data page: https://www.michigan.gov/coronavirus/stats/k-to-12-aged-isd-reporting.

This report is created from data reported by the Michigan Disease Surveillance System (MDSS) the Michigan Department of Health and Human Services' web-based communicable disease reporting system. Data is updated every week.

The purpose of the dashboard is to report the 7 day average of COVID-19 cases in Michigan school students aged 5 through 18 at the intermediate school district and school district levels, as well as display trends over time to better illustrate the changing nature of the COVID-19 pandemic. Cases are attributed to …

Myc Regulates A Pan-Cancer Network Of Co-Expressed Oncogenic Splicing Factors., Laura M Urbanski, Mattia Brugiolo, Sunghee Park, Brittany Lynn Angarola, Nathan Leclair, Marina Yurieva, Phil Palmer, Sangram Keshari Sahu, Olga Anczuków

Faculty Research 2022

MYC is dysregulated in >50% of cancers, but direct targeting of MYC has been clinically unsuccessful. Targeting downstream MYC effector pathways represents an attractive alternative. MYC regulates alternative mRNA splicing, but the mechanistic links between MYC and the splicing machinery in cancer remain underexplored. Here, we identify a network of co-expressed splicing factors (SF-modules) in MYC-active breast tumors. Of these, one is a pan-cancer SF-module correlating with MYC activity across 33 tumor types. In mammary cell models, MYC activation leads to co-upregulation of pan-cancer module SFs and to changes in >4,000 splicing events. In breast cancer organoids, co-overexpression of the …

Acafinder: Genome Mining For Anti-Crispr-Associated Genes, Bowen Yang, Jinfang Zheng, Yanbin Yin

Food for Health: Publications

Anti-CRISPR (Acr) proteins are encoded by (pro)viruses to inhibit their host’s CRISPR-Cas systems. Genes encoding Acr and Aca (Acr associated) proteins often colocalize to form acr-aca operons. Here, we present AcaFinder as the first Aca genome mining tool. AcaFinder can (i) predict Acas and their associated acr-aca operons using guilt-by-association (GBA); (ii) identify homologs of known Acas using an HMM (Hidden Markov model) database; (iii) take input genomes for potential prophages, CRISPR-Cas systems, and self-targeting spacers (STSs); and (iv) provide a standalone program (https://github.com/boweny920/AcaFinder) and a web server (http://aca .unl.edu/Aca). AcaFinder was applied to mining over …