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Proceedings - Wright State University Boonshoft School Of Medicine Seventh Annual Medical Student Research Symposium: Celebrating Medical Student Scholarship, Office Of Research Affairs, Boonshoft School Of Medicine

Medical Student Research Symposium Proceedings

The student abstract booklet is a compilation of abstracts from students' oral and poster presentations at Wright State University's Seventh Annual Boonshoft School of Medicine Medical Student Research Symposium held on April 8, 2015.

Clinical Evaluation Of Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Neisseria Meningitidis In Cerebrospinal Fluid, Dokyung Lee, Eun Jin Kim, Paul E. Kilgore, Soon Ae Kim, Hideyuki Takahashi, Makoto Ohnishi, Dang Duc Anh, Bai Qing Dong, Jung Soo Kim, Jun Tomono, Shigehiko Miyamoto, Tsugunori Notomi, Dong Wook Kim, Mitsuko Seki

Department of Pharmacy Practice

Background

Neisseria meningitidis (Nm) is a leading causative agent of bacterial meningitis in humans. Traditionally, meningococcal meningitis has been diagnosed by bacterial culture. However, isolation of bacteria from patients’ cerebrospinal fluid (CSF) is time consuming and sometimes yields negative results. Recently, polymerase chain reaction (PCR)-based diagnostic methods of detecting Nm have been considered the gold standard because of their superior sensitivity and specificity compared with culture. In this study, we developed a loop-mediated isothermal amplification (LAMP) method and evaluated its ability to detect Nm in cerebrospinal fluid (CSF).

Methodology/Principal Findings

We developed a meningococcal LAMP assay (Nm LAMP) that targets …

Minutes Of 04/08/2015 Hsc Board Of Directors Mtg, Patrice Martin

HSC Committee (A subcommittee of the UNM Board of Regents)

No abstract provided.

Engineering Of A Light-Gated Potassium Channel, Christian Cosentino, Laura Alberio, Sabrina Gazzarrini, Marco Aquila, Eduardo Romano, Solei Cermenati, Paolo Zuccolini, Jan Petersen, Monica Beltrame, James L. Van Etten, John M. Christie, Gerhard Thiel, Anna Moroni

James Van Etten Publications

The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K+) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light–induced K+ channel 1 (BLINK1) engineered by fusing the plant LOV2-Ja photosensory module to the small viral K+ channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K+ selectivity and high single-channel conductance, but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K+ equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish …

Notice Of 04/08/2015 Hsc Board Of Directors Mtg, Patrice Martin

HSC Committee (A subcommittee of the UNM Board of Regents)

No abstract provided.

Agenda Of 04/08/2015 Hsc Board Of Directors Mtg, Patrice Martin

HSC Committee (A subcommittee of the UNM Board of Regents)

No abstract provided.

Live Imaging Of The Ependymal Cilia In The Lateral Ventricles Of The Mouse Brain, Alzahra J. Al Omran, Hannah C. Saternos, Tongyu Liu, Surya M. Nauli, Wissam A. Aboualaiwi

Pharmacy Faculty Articles and Research

Multiciliated ependymal cells line the ventricles in the adult brain. Abnormal function or structure of ependymal cilia is associated with various neurological deficits. The current ex vivo live imaging of motile ependymal cilia technique allows for a detailed study of ciliary dynamics following several steps. These steps include: mice euthanasia with carbon dioxide according to protocols of The University of Toledo’s Institutional Animal Care and Use Committee (IACUC); craniectomy followed by brain removal and sagittal brain dissection with a vibratome or sharp blade to obtain very thin sections through the brain lateral ventricles, where the ependymal cilia can be visualized. …

Portrait De La Nanotechnologie Au Québec Dans Les Milieux Industriels Et De La Recherche Universitaire Et Publique, Charles-Anica Endo, Claude Ostiguy, N. Inès Dossa, Claude Émond

Rapports de recherche scientifique

La dernière décennie a vu émerger un intérêt croissant pour le développement et l’utilisation des nanotechnologies (NT) dans différents domaines incluant la chimie, l’électronique, les cosmétiques et le diagnostic/traitement médical. En raison des propriétés uniques des nanoparticules (NP), de leurs multiples applications, de leur potentiel économique et social, et des percées scientifiques dont elles sont à l’origine, plusieurs gouvernements ont décidé d’investir massivement afin de soutenir la recherche, de favoriser l’implantation industrielle et de s’approprier une part de ce marché colossal. Par contre, les efforts de recherche consacrés à caractériser les effets potentiels de l’utilisation de ces matériaux sur la …

Troubles Musculosquelettiques Et Santé Psychologique : Démarche De Soutien Aux Activités De Prise Et De Répartition Des Appels D’Urgence 9-1-1, Georges Toulouse, Louise St-Arnaud, Mariève Pelletier

Rapports de recherche scientifique

La présente étude constitue un prolongement des recherches antérieures ayant pour objectif de réduire les risques psychosociaux associés aux troubles musculosquelettiques et de santé psychologique chez les préposés des centres d’urgence 9-1-1. Elle se fonde plus précisément sur la précédente recherche (Toulouse et coll., 2011) dont les résultats suggéraient la nécessité de mieux soutenir les préposés aux prises avec des situations d’appels difficiles. En effet, les résultats montraient que les situations, qui génèrent une charge de travail ou une charge cognitive plus élevée sont corrélées à l’augmentation de l’intensité des douleurs au cou et aux épaules, alors que les douleurs …

Promoting Maternal-Newborn Bonding During The Postpartum Period, Kristen L. Baber

Senior Honors Theses

The bond between a mother and her newborn is one of strength, power, and potential. Several factors take place during the early postpartum period that can have a large influence on the bonding that occurs during this time. Oxytocin plays a vital role in the chemistry aspect of bonding, and its effects can be enhanced by a variety of techniques. Psychological theories such as the John Bowlby and Mary Ainsworth’s Theory of Attachment and Erik Erikson’s developmental stage of trust versus mistrust can be applied to the bonding process. The abundant benefits of bonding for both mother and newborn are …

The Meaning Of Patient-Nurse Interaction For Older Hospitalized Women: A Phenomenological Study, Deborah Mize

Doctor of Education (EdD)

The purpose of this dissertation is was to explore how older women perceived their interactions with nurses after receiving care in a hospital setting. Older women are a vulnerable population subject to being overlooked or misunderstood by the nurses caring for them, hence the importance of this study. To gather the data on older women’s lived world of nursing care, I used a phenomenological approach based on a hermeneutic framework that considers linguistic, historical, and social factors to interpret older women’s lived world of nursing care while hospitalized. I personally interviewed seven women between the ages of 66 and 81 …

The Complementary Effects Of Empathy And Nonverbal Communication Training On Persuasion Capabilities, Robin T. Peterson, James M. Leonhardt

Administrative Issues Journal

This paper investigates the possible complementary effects that training in empathy and nonverbal communication may have on persuasion capabilities. The narrative considers implications from the literature and describes an exploratory study in which students, in a managerial setting, were trained in empathy and nonverbal communication. Subsequent evaluations of these students by faculty evaluators and the students themselves provide preliminary evidence that training in empathy, on the one hand, and nonverbal communication on the other can be effective, but concurrent training in both of these is superior to concentration in only one. This is the first research report which deals with …

Src-Family Protein Tyrosine Kinase Phosphorylates Wnk4 And Modulates Its Inhibitory Effect On Kcnj1 (Romk), Dao-Hong Lin, Peng Yue, Orlando Yarborough, Ute I. Scholl, Gerhard Giebisch, Richard P. Lifton, Jesse Rinehart, Wen-Hui Wang

NYMC Faculty Publications

With-no-lysine kinase 4 (WNK4) inhibits the activity of the potassium channel KCNJ1 (ROMK) in the distal nephron, thereby contributing to the maintenance of potassium homeostasis. This effect is inhibited via phosphorylation at Ser1196 by serum/glucocorticoid-induced kinase 1 (SGK1), and this inhibition is attenuated by the Src-family protein tyrosine kinase (SFK). Using Western blot and mass spectrometry, we now identify three sites in WNK4 that are phosphorylated by c-Src: Tyr(1092), Tyr(1094), and Tyr(1143), and show that both c-Src and protein tyrosine phosphatase type 1D (PTP-1D) coimmunoprecipitate with WNK4. Mutation of Tyr(1092) or Tyr(1143) to phenylalanine decreased the association of c-Src or …

Human Severe Sepsis Cytokine Mixture Increases Beta 2-Integrin-Dependent Polymorphonuclear Leukocyte Adhesion To Cerebral Microvascular Endothelial Cells In Vitro, Chris Blom, Brittany L. Deller, Douglas D. Fraser, Eric K. Patterson, Claudio M. Martin, Bryan Young, Patricia C. Liaw, Payam Yazdan-Ashoori, Angelica Oritz, Brian Webb, Greg Kilmer, David E. Carter, Gediminas Cepinskas

Anatomy and Cell Biology Publications

Introduction: Sepsis-associated encephalopathy (SAE) is a state of acute brain dysfunction in response to a systemic infection. We propose that systemic inflammation during sepsis causes increased adhesion of leukocytes to the brain microvasculature, resulting in blood-brain barrier dysfunction. Thus, our objectives were to measure inflammatory analytes in plasma of severe sepsis patients to create an experimental cytokine mixture (CM), and to use this CM to investigate the activation and interactions of polymorphonuclear leukocytes (PMN) and human cerebrovascular endothelial cells (hCMEC/D3) in vitro. Methods: The concentrations of 41 inflammatory analytes were quantified in plasma obtained from 20 severe sepsis patients and …

Best Nursing Interventions For Educating Parents On Trtosinemia Type I, Julianna Mize

Research Horizons Day Posters

No abstract provided.

How Women Manage Their Pain Non-Pharmacologically During Labor Outside The Hospital Setting, Sarah Christine Schueler

Research Horizons Day Posters

No abstract provided.

Pain, Agitation, And Delirium In The Intensive Care Unit, Elizabeth A. Bailey, Theresa Harris, Haley Hoy, Sandra Carpenter

Research Horizons Day Posters

No abstract provided.

How Can Utilizing Signage And Technology Help Minimize Interruptions For Postpartum Breastfeeding Mothers?, Mary Owens

Research Horizons Day Posters

No abstract provided.

Exploring The Transition From Hospital To Home In The Older Adult Population, Sharon Coffey

Research Horizons Day Posters

No abstract provided.

Analyzing The Presence Of Bacterial Colonies Within A Neonatal Suctioning Device: The Blue-Bulb Syringe, N. Edward Damron

Research Horizons Day Posters

No abstract provided.

Practical Management Of Anticoagulation In Patients With Atrial Fibrillation., Richard J Kovacs, Greg C Flaker, Sherry J Saxonhouse, John U. Doherty, Kim K Birtcher, Adam Cuker, Bruce L Davidson, Robert P Giugliano, Christopher B Granger, Amir K Jaffer, Bella H Mehta, Edith Nutescu, Kim A Williams

Department of Medicine Faculty Papers

Anticoagulation for atrial fibrillation has become more complex due to the introduction of new anticoagulant agents, the number and kinds of patients requiring therapy, and the interactions of those patients in the matrix of care. The management of anticoagulation has become a "team sport" involving multiple specialties in multiple sites of care. The American College of Cardiology, through the College's Anticoagulation Initiative, convened a roundtable of experts from multiple specialties to discuss topics important to the management of patients requiring anticoagulation and to make expert recommendations on issues such as the initiation and interruption of anticoagulation, quality of anticoagulation care, …

Proteolysis Of Virulence Regulator Toxr Is Associated With Entry Of Vibrio Cholerae Into A Dormant State, Salvador Almagro-Moreno, Tae K. Kim, Karen Skorupski, Ronald K. Taylor

Dartmouth Scholarship

Vibrio cholerae O1 is a natural inhabitant of aquatic environments and causes the diarrheal disease, cholera. Two of its primary virulence regulators, TcpP and ToxR, are localized in the inner membrane. TcpP is encoded on the Vibrio Pathogenicity Island (VPI), a horizontally acquired mobile genetic element, and functions primarily in virulence gene regulation. TcpP has been shown to undergo regulated intramembrane proteolysis (RIP) in response to environmental conditions that are unfavorable for virulence gene expression. ToxR is encoded in the ancestral genome and is present in non-pathogenic strains of V. cholerae, indicating it has roles outside of the human …

The Effects Of Nutrition And Physical Activity Education On Knowledge And Glycemic Control Among Persons With Type 2 Diabetes, Alexandra Harris

Evidence-Based Practice Project Reports

Diabetes affects millions of people worldwide. Approximately 29.1 million people or 9.3% of the United States population has diabetes (Centers for Disease Control (CDC), 2014). Diabetes was the seventh leading cause of death in the United States in 2010 and is projected by the World Health Organization (WHO) to be the seventh leading cause of death globally by 2030. The purpose of this evidence-based project was to provide nutrition and physical activity education in an effort to improve diabetes knowledge and glycemic control among persons with type 2 diabetes. Hemoglobin A1c levels and Diabetes Knowledge Test scores were compared from …

Myocardial Feature Tracking Reduces Observer-Dependence In Low-Dose Dobutamine Stress Cardiovascular Magnetic Resonance., Andreas Schuster, Matthias Paul, Nuno Bettencourt, Shazia T. Hussain, Geraint Morton, Shelby Kutty, Boris Bigalke, Amedeo Chiribiri, Divaka Perera, Eike Nagel, Philipp Beerbaum

Journal Articles: Cardiology

OBJECTIVES: To determine whether quantitative wall motion assessment by CMR myocardial feature tracking (CMR-FT) would reduce the impact of observer experience as compared to visual analysis in patients with ischemic cardiomyopathy (ICM).

METHODS: 15 consecutive patients with ICM referred for assessment of hibernating myocardium were studied at 3 Tesla using SSFP cine images at rest and during low dose dobutamine stress (5 and 10 μg/kg/min of dobutamine). Conventional visual, qualitative analysis was performed independently and blinded by an experienced and an inexperienced reader, followed by post-processing of the same images by CMR-FT to quantify subendocardial and subepicardial circumferential (Eccendo and …

Sexual Orientation Competence: Psychologists’ Perceived Competence And Relationships To Multicultural Competence, Training, Engagement, And Exposure To Lesbian, Gay, And Bisexual Individuals, Marty A. Cooper

Seton Hall University Dissertations and Theses (ETDs)

Attitudes toward sexual orientation, specifically towards those who identify as lesbians, gay men, and bisexual (LGB) individuals, have varied temporally. Given the pervasiveness of heterosexism in American culture, it becomes quite important to understand if we, as psychologists, could unintentionally create heterosexist environments with our clients. This study looked at the following research question: What are the unique contributions of the predictor variables: exposure to persons of sexual minority status, training experience in sexual minority populations, perceived multicultural competence, and engagement in sexual minority specialization and practice to the criterion variable perceived sexual orientation competence within United States (US) licensed …

Engaging A ‘Scared Population’ Of Huntington’S Disease Families By Respecting Their Journeys, Kenneth P. Serbin

At Risk for Huntington's Disease

No abstract provided.

Online Searching In Pbl Tutorials, Jun Jin, Susan M. Bridges, Michael G. Botelho, Lap Ki Chan

Interdisciplinary Journal of Problem-Based Learning

This study aims to explore how online searching plays a role during PBL tutorials in two undergraduate health sciences curricula, Medicine and Dentistry. Utilizing Interactional Ethnography (IE) as an organizing framework for data collection and analysis, and drawing on a critical theory of technology as an explanatory lens, enabled a textured understanding of student practices and beliefs regarding online searching during face-to-face PBL tutorials. Two event maps trace key transitions in learning regarding online searching in one cycle of problem-based learning in each program. From a critical perspective, analysis of students’ stimulated recall interviews indicated that the use of students’ …

Motivational Influences Of Using Peer Evaluation In Problem-Based Learning In Medical Education, Sara Abercrombie, Jay Parkes, Teresita Mccarty

Interdisciplinary Journal of Problem-Based Learning

This study investigates the ways in which medical students’ achievement goal orientations (AGO) affect their perceptions of learning and actual learning from an online problem-based learning environment, Calibrated Peer Review^TM. First, the tenability of a four-factor model (Elliot & McGregor, 2001) of AGO was tested with data collected from medical students (N = 137). Then, a structural regression model relating the factors of AGO to students’ perceptions of grading fairness, judgments of learning, and scoring accuracy was tested. The results indicate that student engagement and success in diagnosing a patient’s presentation using a peer feedback-rich web-based PBL environment …

The Use Of Twitter In The Creation Of Educational Professional Learning Opportunities, Carrie R. Ross, Robert M. Maninger, Kimberly N. Laprairie, Sam Sullivan

Administrative Issues Journal

This study sought to examine how educators are using Twitter to increase their professional learning opportunities beyond the boundaries of traditional professional development offers, and whether educators feel a greater sense of fulfillment receiving professional development through networking and community learning than they do through traditional means of learning. A population of 160 educators—105 females and 55 males between the ages of 22 and 65—were surveyed using education related hashtags on Twitter. Thirty-two educators from the survey population elected to participate in an interview. The study discovered that educators are frequently using Twitter professionally to collaborate, network, and engage in …

What's Happening: April 6, 2015, Maine Medical Center

What's Happening

No abstract provided.