Medicine and Health Sciences Commons^™

Novel Insights Into Ubiquitin-Like Protein E1-E2 Interactions, Asad Taherbhoy

Theses and Dissertations (ETD)

Posttranslational modification of macromolecules by ubiquitin-like proteins (UBLs) such as ubiquitin, Sumo and NEDD8 regulate a vast array of processes in the cell. Transfer of UBLs to their target generally occurs by a series of molecular handoffs down an E1‑E2‑E3 cascade. We are interested in understanding how E1‑E2 pairs interact and mediate UBL transfer. To this effect, we studied two E1‑E2 pairs: the Sumo pathway (Sumo utilizes a canonical E1 and E2) and the Atg8 pathway (Atg8 is a UBL involved in autophagy that utilizes a non-canonical E1‑E2 pair).

Sumo conjugation is initiated by the heterodimeric Aos1‑Uba2 E1 enzyme (in …

Use Of A Multi-Axis Robotic Testing Platform To Investigate The Sagittal Mechanics Of The Multi-Body Lumbar Spine, Jessica Rose Stubbs

Theses and Dissertations (ETD)

A biomechanical study was performed to compare range of motion of the multi-body lumbar spine using three different protocols: pure moment, eccentric loading, and a new method called combined loading moment. The objectives of the study were to introduce a new protocol that overcomes the limitations of previous methods by applying more realistic loading conditions and to compare the range of motion of this new protocol to those of eccentric loading and pure moment protocols within the same specimen pool. The second objective of this study was to compare the data sets of these three protocols to both in vivo …

Pten Function In Postnatal Brain Development And Tumorigenesis, Guo Zhu

Theses and Dissertations (ETD)

PTEN (Phosphatase and Tensin homolog deleted on chromosome 10) is a tumor suppressor gene that is commonly mutated in multiple types of human cancers including endometrial, prostate, breast cancers and glioblastoma multiforme (GBM). PTEN functions as a phospholipid phosphatase antagonizing phosphorylation by phosphatidylinositol‑3‑OH kinases (PI3Ks) and thus serves as a major negative regulator of the PI3K pathway. Homozygous Pten knock-out mice die during embryogenesis. Conditional Pten knock-out in the nervous system showed that Pten negatively regulates self-renewal and proliferation of neural stem cells, negatively controls neuronal size, is required for proper neuronal positioning and is involved in the development and …

Systemic Aav-Mediated Gene Therapy Using Epo-R76e To Protect Retinal Ganglion Cells From Optic Nerve Injury And Disease, Timothy A. Sullivan

Theses and Dissertations (ETD)

Neuroprotection is a strategy to prevent or reduce neuronal cell death regardless of the underlying cause. Erythropoietin (EPO) exhibits neuroprotective effects in a variety of in vitro and in vivo models of neuronal cell death although its classical function is the regulation of red blood cell production. EPO crosses the blood brain barrier and therefore can be delivered systemically. However, long term treatment with exogenous EPO causes over stimulation of the erythropoietic pathway which can be lethal. The erythropoietic and neuroprotective pathways are regulated by different receptors; further, it is possible to modify EPO to primarily, if not solely, activate …

Effect Of Occlusion, Directionality And Age On Horizontal Localization, Lynzee Nicole Alworth

Theses and Dissertations (ETD)

Localization acuity of a given listener is dependent upon the ability discriminate between interaural time and level disparities. Interaural time differences are encoded by low frequency information whereas interaural level differences are encoded by high frequency information. Much research has examined effects of hearing aid microphone technologies and occlusion separately and prior studies have not evaluated age as a factor in localization acuity. Open-fit hearing instruments provide new earmold technologies and varying microphone capabilities; however, these instruments have yet to be evaluated with regard to horizontal localization acuity.

Thus, the purpose of this study is to examine the effects of …

The Biochemical Pathway Leading To Lpa Generation Upon Blood Coagulation, Alyssa Lynn Jefferson Bolen

Theses and Dissertations (ETD)

Platelet activation initiates an upsurge in 18:2 and 20:4 lysophosphatidic acid (LPA) production. The biochemical pathway responsible for LPA production during blood clotting is not fully understood. We have purified a phospholipase A₁ (PLA₁) from thrombin-activated human platelets using sequential chromatographic steps followed by fluorophosphonate‑biotin affinity labeling and proteomics. We identified acyl‑protein thioesterase 1 (aka. lysophospholipase A1, accession code O75608) as a novel PLA₁. Addition of this recombinant PLA₁ significantly increased the production of sn‑2‑esterified polyunsaturated LPCs and the corresponding LPAs in plasma. We next examined the regioisomeric preference of lysophospholipase …

The Influence Of Patient-Reported Provider Availability Factors On Nonurgent Emergency Department Use, Cheryl Anne Dewood

Theses and Dissertations (ETD)

Purpose. The purpose of this study was to examine the influence of patient‑perceived provider availability on nonurgent emergency department use (NUEDU). The study population consisted of a nationally‑representative population of non‑elderly adults who were continuously privately insured (CPI) for at least a year before an emergency department visit that was determined to be nonurgent, and who reported having a regular source of care (RSC).

Methods. Data were obtained from a nationally‑representative longitudinal survey, the Medical Expenditure Panel Survey (MEPS). The classification of ED visits as nonurgent was based on patient report using a method developed for this study. Patient­‑perceived provider …

Analysis Of Out-Of-Pocket Expenditures Of Oral Oncologics For Tennessee Recipients Of Medicare Part D, Eve Carolyn Elias

Theses and Dissertations (ETD)

Background: There are few studies that examine patients' costs of oral oncology drugs from Medicare Part D plan providers. These drugs can impose a financial burden to beneficiaries, and, due to their cost, place beneficiaries in the coverage gap in a short period of time. In addition to examining costs, there is little published concerning the potential impact of drug utilization management techniques on access to these drugs in Medicare Part D plans. This study examined the roles of prior authorization, step-therapy, and placement of drugs in higher formulary tiers on patients' out-of-pocket costs, and access to therapy.

Methods …

It’S Time To Teach Back: An Inter-Professional Approach To Enhance Learning And Reduce Readmissions, Debra Peter Msn, Rn, Bc, Cmsrn, Paula Robinson Msn, Rn, Bc

Patient Care Services / Nursing

No abstract provided.

Examining Nursing Presence In The Acute Care Setting As An Indicator Of Patient Satisfaction With Nursing Care, Wendy Hansbrough Phd, Msn, Rn

Dissertations

Nursing presence is conceptualized as occurring within the nurse-patient relationship when the nurse acknowledges the uniqueness of the patient, within his or her context of being and chooses to intervene on the patient's behalf with a patient who allows the nurse into a reciprocal relationship. Nursing presence is described as occurring in varying levels and nurse expertise is suggested as one antecedent. Quantification of nursing presence would be useful in examination of nursing care outcomes, quality management and research. The Presence of Nursing Scale, PONS (Kostovich, 2002) was used in this study to test its reliability and determine its validity …

The Post Deployment Lived Experience Of U.S. Military Troops After Combat-Related Blast Exposure, Shirely A. Jett Phd

Dissertations

Blast-induced neurotrauma (BINT) is a newly emerging re-occurrence of an old combat-related injury in U.S. military troops returning from deployment in Afghanistan and Iraq. BINT is leading to a silent epidemic of symptomatic troops who face barriers to accessing healthcare and suffer debilitating symptoms in silence. The purpose of this hermeneutic phenomenological study was to discover the meaning of the lived experience of U.S. troops returning from Afghanistan and Iraq after combat-related blast exposure. Eleven service members and veterans ranging in age from 21 to over 30 years old participated in semi-structured face to face interviews. These service members and …

Emergence Delirium In U.S. Military Combat Veterans, Jason M. Mcguire Phd, Crna

Dissertations

This dissertation study examined the phenomenon of emergence delirium in U.S. military combat veterans. Emergence delirium is a post-anesthetic phenomenon that occurs immediately following emergence from general anesthesia and is characterized by agitation, confusion, and violent physical and/or verbal behavior. Clinical evidence suggests that emergence delirium is occurring with greater frequency among military personnel returning from conflict in the Middle East. This body of work is a culmination of three distinctive phases and is presented in three individual manuscripts. The first manuscript, "Risk Factors for Emergence Delirium in U.S. Military Members," was published in the Journal of PeriAnesthesia Nursing with …

Nutritional Status And Clinical Outcomes Of Residents Admitted To A Nursing Home, Sheryl Nespor Phd

Dissertations

Purpose The purpose of this research study was to describe the nutritional status (well nourished, at risk for malnutrition, or malnourished) of residents admitted to nursing homes and adverse clinical outcomes in those residents after four weeks. Methodology A descriptive, correlational design utilizing medical record data was used to examine the relationship between nutritional status and adverse clinical outcomes of weight loss and pressure ulcers. Results The sample (n=69) was predominately Caucasian (90%) with 38% having one or more pressure ulcers on admission. Most of the residents were admitted from an acute care facility (97%) and were either malnourished or …

California School Nurse Survey, Rachel Van Niekerk Phd

Dissertations

The purpose of this study was to obtain evidence to support the assumption that school nurses have a positive effect on school attendance and medication administration practices and to hear the voice of the California school nurse. Data from this exploratory, descriptive study came from an online researcher developed survey consisting of both forced choice and open-ended questions completed by California school nurses representing the three major regions of California (Northern, Central, and Southern). The following three research questions guided the study: 1) What is the relationship between school and nurse factors on student outcomes? 2) How valued do California …

A Quantitative Inquiry Into Moral Distress And Psychological Empowerment In Critical Care Nurses Caring For Adults During End Of Life, Annette Marie Browning Phd, Rn, Cns

Dissertations

Background Critical care nurses care for many adults at end of life and it is known that these nurses can experience moral distress. Moral distress occurs when nurses are unable to perform what they believe is ethically correct. There has been speculation that psychological empowerment may ameliorate moral distress among critical care nurses. However, research has yet to examine the relationship between moral distress and psychological empowerment in the population of critical care nurses.

Objectives To describe the relationships between moral distress, psychological empowerment and select demographics in critical care nurses caring for patients at EOL.

Method Using a quantitative …

Leveraging Complex Event Processing For Smart Hospitals Using Rfid, Wen Yao, Chao-Hsien Chu, Zang Li

Research Collection School Of Computing and Information Systems

RFID technology has been examined in healthcare to support a variety of applications such as patient identification and monitoring, asset tracking, and patient–drug compliance. However, managing the large volume of RFID data and understanding them in the medical context present new challenges. One effective solution for dealing with these challenges is complex event processing (CEP), which can extract meaningful events for context-aware applications. In this paper, we propose a CEP framework to model surgical events and critical situations in an RFID-enabled hospital. We have implemented a prototype system with the proposed approach for surgical management and conducted performance evaluations to …

Mitigation Of Bovine Respiratory Disease Complex: Interactions Of Physiology, Immunology, Nutrition And Management, John Thomas Richeson

Graduate Theses and Dissertations

A series of experiments were conducted to determine the effects of on-arrival vs. delayed respiratory vaccination and exposure to persistently infected bovine viral diarrhea virus challenge on health, gain performance, and physiological and immunological measurements in newly received stocker cattle. Two experiments evaluated timing (d 0 vs. 14) of respiratory, clostridial, or both vaccinations in newly received stocker calves during stress-induced immunosuppression. In Exp. 1, calves receiving 14-d delayed vaccination of a pentavalent modified-live respiratory virus (MLV) vaccine had greater ADG during the 42-d receiving period and antibody titers against infectious bovine rhinotracheitis on d 42; however, morbidity rate did …

The Effects Of A Lumbopelvic Hip Complex Conditioning Program On Dynamic Knee Valgus In Female Tennis Players, Katelyn Marie Roling

Graduate Theses and Dissertations

Female athletes have a four-to-six times greater risk of sustaining a lower extremity injury than their male counterparts (Hewett, 2000). One explanation for the discrepancy in injury rates is increased dynamic knee valgus angles caused by neuromuscular imbalances of the lumbopelvic hip complex (LPHC). The purpose of this study was to determine if implementing a LPHC conditioning program improved neuromuscular deficiencies. Seven female collegiate tennis players competitive at the NCAA Division I level volunteered for the study (19.7 + .95 years, 167.3 + 7.4 cm, and 60.72 kg + 6.71 kg). The subjects participated in a 16 week LPHC conditioning …

College Student Mental Health: The Relationship Between Depression And Emotional Intelligence Using The Student Relationships Assessment, Andre George Broquard

Graduate Theses and Dissertations

The purpose for conducting this study was to investigate the relationship between emotional relational intelligence (ERQ) and depression in college students. The significance of this study is based on the additional support that can be provided to students with increased information and understanding of emotional relational intelligence and depression. In an effort to help college students achieve their educational goals and aspirations, the researcher suggests that emotional relational intelligence can be beneficial. The relationship between depression and emotional relational intelligence may provide insight on how to support and care for college students who are struggling with depressive symptomology. The results …

Chemical Ecology, Population Dynamics And Insecticide Susceptibility Of Lesser Mealworm Alphitobius Diaperinus (Panzer) (Coleoptera: Tenebrionidae), Narinderpal Singh

Graduate Theses and Dissertations

The lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), is a serious, cosmopolitan pest present in poultry production facilities, where it slows down weight gain in chicks, carries disease–causing organisms, and causes damage to poultry insulation. The first study estimated that the lesser mealworm densities in poultry litter applied to pastures were 3.5, 2.2 and 0.4 million larvae, adults and pupae, respectively, per hectare in Savoy, Arkansas. The repercussions of these adult beetles are that they often disperse to and reinfest poultry houses with the potential to vector disease to poultry, and disperse to invade human dwellings resulting in litigation. The …

Pembuatan Sediaan Tablet Mengapung Famotidin Menggunakan Kompleks Polielektrolit Kitosan-Pektin Sebagai Bahan Matriks, Erny Sagita, Effionora Anwar, Silvia Surini

Majalah Ilmu Kefarmasian

Floating tablet is one of the drug delivery system retained in the stomach which aims to prolong the lag time of the drug in the stomach. This principle can be used to improve the efficacy of famotidine in treating diseases in the stomach The purpose of this research is to develop dosage form afloat by using Chitosan Pectin Electrolytes (PEC) as matrix then used as the matrix in tablet dosage form with famotidine as a drug model. PEC also combined with hydroxypropylmethylcellulose (HPMC) with different. PEC was also combined with hydroxypropilmethylcellulose (HPMC) in different concentrations. The results of the dissolution …

Pengembangan Sistem Penghantaran Obat Solid Self-Emulsifying Mikropartikel Gliklazid Untuk Meningkatkan Disolusi, Mega Suryani, Sutriyo Sutriyo

Majalah Ilmu Kefarmasian

Gliclazide is a Hypoglycemicdrug, second-generation sulfonylurea used in the treatment of non insulin dependent diabetes mellitus. Gliclazide exhibit poor aqueous solubility, so that their low bioavailability. Objectivesthis study is to develop solid self-emulsifying (SE) microparticles of poorly soluble gliclazide to dissolution enchancement. These microparticles were prepared by spray drying method, using three surfactant (acripol, tween 80 and sodium lauryl sulphate) with various concentration. SE microparticles characterized in terms of morphology (SEM), particle size distribution (PSA), x-ray diffraction (XRD), thermal analysis (DSC), functional group analysis (FTIR), solubility test, and dissolution test. The all gliclazide SE microparticlesexhibited particle size smaller than pure …

Analisis Adduct Dna Setelah Pemberian Natrium Nitrit Dan Dimetilamin Secara Berulang Pada Tikus, Sherly Meilianti, Yahdiana Harahap, Hayun Hayun

Majalah Ilmu Kefarmasian

Nitrosodimethylamine is a carcinogenic compound which can be formed from the reaction of nitrite and dimethylamine that is found in food. Nitrosodimethylamineis activated in liver and alkylates the DNA base and producing a DNA adductssuch as O6-methylguanine and N7-methylguanine that have a role incarcinogenesis. In this research, DNA was isolated from rat’s blood which waspreviously given nitrosodimethylamine’s precursor, sodium nitrite anddimethylamine. DNA adducts can be obtained from hydrolysis in hydrochloricacid 0.1 N for 30 minutes at 7000C. Then the adducts were analyzed using High Performance Liquid Chromatography (HPLC), with a strong cation exchangecolumn (Supelcosil LC-SCX, 5 μm, 250 x 4.6 …

Efek Antidiabetes Dan Identifikasi Senyawa Dominan Fraksi Kloroform Herba Ciplukan (Physalis Angulata L.), Sediarso Sediarso, Hadi Sunaryo, Nurul Amalia

Majalah Ilmu Kefarmasian

Antidiabetic activity of ciplukan (Physalis angulataL.) herb has a lot to do, as an extracts and infusions, proven to reduce blood sugar levels in rats or mice induced by alloxan. The research objective was to evaluate the antidiabetic effect and identify the content of the chloroform fraction ciplukan herb eluated with methanol-ammonia. Antidiabetic effects testing conducted using 30 male white mice (Mus musculus) ddY strain were divided into 6 groups, each consisting of 5 mice. K-1 is a negative control induced by alloxan, K-2, K-3 and K-4 induced by alloxan and treated with the test substance dose of 50 mg/kg …

Allied Health, Carol L. Watwood

DLPS Faculty Publications

No abstract provided.

Adolescent Medicine, Carol L. Watwood

DLPS Faculty Publications

No abstract provided.

Radiologic Technology, Carol L. Watwood

DLPS Faculty Publications

No abstract provided.

Agenda (Pdf), School Of Dentistry

LSU School of Dentistry Research Day

No abstract provided.

A Compact Guide To The Diagnosis And Management Of Neurofibromatosis Type 1, Timothy Beer, David Strayer, Md, Phd

Department of Pathology, Anatomy, and Cell Biology Resident's Posters

Brief Introduction

Neurofibromatosis type 1 (NF-1) is a multisystem neurocutaneous disorder resulting from mutations in the NF-1 gene on chromosome 17 (q11.2). Mutations in NF-1 result in deficient activity of the tumor suppresor protein neurofibromin, allowing for uncontrolled constitutive activity of the proliferative Raspathway in several neural cell types, including neurons, oligodendrocytes, astrocytes and Schawnn cells. Half of NF-1 cases are inherited in autosomal dominant fashion, while half develop sporadically. The syndrome has 100% penetrance but highly variable expressivity. The prevalence of this syndrome is 1/3,000, making it common enough to warrant YOU, the physician, taking a moment to learn …

Transplantation Of Adult Mouse Ips Cell-Derived Photoreceptor Precursors Restores Retinal Structure And Function In Degenerative Mice, Budd A. Tucker, In-Hyun Park, Sara D. Qi, Henry J. Klassen, Caihui Jiang, Jing Yao, Stephen Redenti, George Q. Daley, Michael J. Young

Publications and Research

This study was designed to determine whether adult mouse induced pluripotent stem cells (iPSCs), could be used to produce retinal precursors and subsequently photoreceptor cells for retinal transplantation to restore retinal function in degenerative hosts. iPSCs were generated using adult dsRed mouse dermal fibroblasts via retroviral induction of the transcription factors Oct4, Sox2, KLF4 and c-Myc. As with normal mouse ES cells, adult dsRed iPSCs expressed the pluripotency genes SSEA1, Oct4, Sox2, KLF4, c-Myc and Nanog. Following transplantation into the eye of immunecompromised retinal degenerative mice these cells proceeded to form teratomas containing tissue comprising all three germ layers. At …