Medicine and Health Sciences Commons^™

Molecular Determinant Of Ion Selectivity Of A (Na+ + K+)-Coupled Rat Brain Glutamate Transporter, Yumin Zhang, Annie Bendahan, Ruth Zarbiv, Michael Kavanaugh, Baruch I. Kanner

Biomedical and Pharmaceutical Sciences Faculty Publications

Glutamate transporters remove this neurotransmitter from the synaptic cleft by a two-stage electrogenic process, in which glutamate is first cotransported with three sodium ions and a proton. Subsequently, the cycle is completed by translocation of a potassium ion in the opposite direction. Recently, we have identified an amino acid residue of the glutamate transporter GLT-1 (Glu-404) that influences potassium coupling. We have now analyzed the effect of seven other amino acid residues in the highly conserved region surrounding this site. One of these residues, Tyr-403, also proved important for potassium coupling, because mutation to Phe (Y403F) resulted in an electroneutral …

Cysteine Scanning Of The Surroundings Of An Alkali-Ion Binding Site Of The Glutamate Transporter Glt-1 Reveals A Conformationally Sensitive Residue, Ruth Zarbiv, Myriam Grunewald, Michael Kavanaugh, Baruch I. Kanner

Biomedical and Pharmaceutical Sciences Faculty Publications

Glutamate transporters remove this transmitter from the extracellular space by cotransport with three sodium ions and a proton. The cycle is completed by translocation of a potassium ion in the opposite direction. Recently we have identified two adjacent amino acid residues of the glutamate transporter GLT-1 that influence potassium coupling. Using the scanning cysteine accessibility method we have now explored the highly conserved region surrounding them. Replacement of each of the five consecutive residues 396–400 by cysteine abolished transport activity but at several other positions the substitution is tolerated. One residue, tyrosine 403, was identified where cysteine substitution renders the …

Gp120 Envelope Glycoproteins Of Human Immunodeficiency Viruses Competitively Antagonize Signaling By Coreceptors Cxcr4 And Ccr5, Navid Madani, Susan L. Kozak, Michael Kavanaugh, David Kabat

Biomedical and Pharmaceutical Sciences Faculty Publications

Signal transductions by the dual-function CXCR4 and CCR5 chemokine receptors/HIV type 1 (HIV-1) coreceptors were electrophysiologically monitored in Xenopus laevis oocytes that also coexpressed the viral receptor CD4 and a G protein-coupled inward-rectifying K⁺ channel (Kir 3.1). Large Kir 3.1-dependent currents generated in response to the corresponding chemokines (SDF-1α for CXCR4 and MIP-1α; MIP-1β and RANTES for CCR5) were blocked by pertussis toxin, suggesting involvement of inhibitory guanine nucleotide-binding proteins. Prolonged exposures to chemokines caused substantial but incomplete desensitization of responses with time constants of 5–7 min and recovery time constants of 12–19 min. CXCR4 and CCR5 exhibited heterologous …

Arachidonic Acid Activates A Proton Current In The Rat Glutamate Transporter Eaat4, Anastassios V. Tzingounis, Chien-Liang Lin, Jeffrey D. Rothstein, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

The excitatory amino acid transporter EAAT4 is expressed predominantly in Purkinje neurons in the rat cerebellum (1-3), and it participates in postsynaptic reuptake of glutamate released at the climbing fiber synapse (4). Transporter-mediated currents in Purkinje neurons are increased more than 3-fold by arachidonic acid, a second messenger that is liberated following depolarization-induced Ca²⁺ activation of phospholipase A₂ (5). In this study we demonstrate that application of arachidonic acid to oocytes expressing rat EAAT4 increased glutamate-induced currents to a similar extent. However, arachidonic acid did not cause an increase in the rate …

Macroscopic And Microscopic Properties Of A Cloned Glutamate Transporter/Chloride Channel, Jacques I. Wadiche, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

The behavior of a Cl⁻ channel associated with a glutamate transporter was studied using intracellular and patch recording techniques in Xenopus oocytes injected with human EAAT1 cRNA. Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhibited strong selectivity for amphipathic anions and had a minimum pore diameter of ∼5Å. Glutamate flux exhibited a much greater temperature dependence than Cl⁻ flux. Stationary and nonstationary noise analysis was consistent with a sub-femtosiemen Cl⁻ conductance and a maximum channelP_o ≪ 1. The glutamate binding rate was similar to estimates …

Occupational Therapy Program 1998, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Postoperative Infection Rates With Instrumented Lumbar Fusion: A Retrospective Review Of 129 Consecutive Cases, Nicole G. Kurzynske, Scott D. Hodges, Stephen C. Humphreys, Jason C. Eck, Laurie C. Covington

Capstone Research Projects

Introduction: The use of internal fixation devices in spinal surgery is common to achieve reduction, maintain alignment, and stabilize the spine while fusion occurs. Infection rates of less than 2% (0-2%) were reported in the late 1960's without the use of instrumentation. Reported rates of infection following instrumented fusion are generally around 6% (range 0-11% ). The purpose of this study was to retrospectively review the incidence of postoperative infection following instrumented spinal fusion to determine if infection rates related to patient type and surgical procedure.

Methods: A retrospective review was conducted of 129 consecutive cases of instrumented lumbar spinal …

Infant Blood Pressure: The Validity Of Calf Measurements, Delia Wessels

Capstone Research Projects

Blood pressure monitoring is critical in the assessment and treatment of hospitalized infants. Two methods are commonly used to obtain measurements: Invasive arterial line measurement and noninvasive cuff measurements. The purpose of this study was to validate previous research supporting the use of noninvasive calf cuff measurements and to compare the values of calf and upper arm blood pressure measurements in hospitalized infants. A descriptive correlational design was used to investigate the convenience sample of 26 infants in a neonatal intensive care unit. Data obtained through chart review was analyzed using the Pearson r with a significance level of0.05. Results …

College Of Osteopathic Medicine Student Handbook, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

College Of Optometry-For The 1998 Entering Class, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Physical Therapy Program 1998, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Educational Mobility, American Association Of Colleges Of Nursing, Cynthia E. Kielinen, Joan L. Creasia, Timothy M. Gaspar, Karen L. Miller, Christine Talmadge

Nursing Faculty Publications

No abstract provided.

Ada News Daily - 1998 Day 4, American Dental Association, Publishing Division

ADA News

The ADA News Daily (also called the ADA News Convention Daily) is a special edition of the ADA News published each day during American Dental Association Annual Sessions.

Ada News Daily - 1998 Day 3, American Dental Association, Publishing Division

ADA News

The ADA News Daily (also called the ADA News Convention Daily) is a special edition of the ADA News published each day during American Dental Association Annual Sessions.

Ada News Daily - 1998 Day 2, American Dental Association, Publishing Division

ADA News

The ADA News Daily (also called the ADA News Convention Daily) is a special edition of the ADA News published each day during American Dental Association Annual Sessions.

เหงือกบวมในผู้ป่วยโรคลมชัก, อรนุช บุญรังสิมันตุ์

Chulalongkorn University Dental Journal

รายงานผู้ป่วยหญิงหนึ่งราย ซึ่งมีเหงือกบริเวณหน้าบนและล่างบวมมาก เนื่องจากได้รับยาไดแลนทีนในการรักษาโรคลมชักที่โรงพยาบาลระยอง ผลข้างเคียงของยาไดแลนทีนทางทันตกรรม คือ การทําให้เหงือกบวมโตได้โดยเฉพาะผู้ที่มีอนามัยช่องปากไม่ดี ดังนั้นในการรักษาผู้ป่วยเหล่านี้ ทันตแพทย์ควรตระหนักถึง การเน้นให้ผู้ป่วยดูแลอนามัยช่องปากเป็นสําคัญ

College Of Osteopathic Medicine-For The 1998 Entering Class, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Ada Constitution & Bylaws (1998), American Dental Association

Constitution & Bylaws

The ADA Commons Constitution & Bylaws archival collection comprises printed issues of the American Dental Association's Constitution and Bylaws. The collection also includes the ADA Charter and the ADA Code of Ethics issued 1924-1946.

1998 Touro College School Of Health Sciences Yearbook, Touro College School Of Health Sciences

Yearbooks and Newsletters

1998 Touro College School of Health Sciences Yearbook

Patent: Male Contraceptive Implant, Alfred J. Moo-Young, Saleh I. Saleh

Formulation/Product Development

The present invention relates to implantable male contraceptive devices. An ethylene vinyl acetate copolymer based implant is described for delivery of an androgen and a system including an ethylene vinyl acetate copolymer based implant as well as a second implantare described for the administration of both androgen and a sterilitant. These implants may be used to provide contraception for men, as well as, for hormone therapy, treatment of enlarged prostate and other aliments.

Virginia Dental Journal (Vol. 75, No. 1, 1998)

Virginia Dental Journal

No abstract provided.

Histochemical And Fluorescent Labelling Ofneurons Projecting To Nucleus Accumbens: Therelation To Pain Processing, Gülgün Kayalioğlu, Figen Gövsa, Vladaimir A. Maisky, Nuran Hari̇ri̇, Berrin Erdem, Yılmaz Şenyilmaz

Turkish Journal of Medical Sciences

This study was undertaken to examine the laminar distribution of spinotelencephalic tract neurons in the rat, by means of retrograde fluorescent labelling and to estimate the role of NADPH-diaphorase-reactive spinal cells as sources of spino-supraspinal pathways in the rat. The localization, size and shape of some neurons were similar in both labelling methods. Cells were mostly multipolar in the medial part of nucl. proprius, and fusiform and spindle-shaped in lamina I and X. It is suggested that some of the NADPH-d (+) neurons in the deep dorsal horn and lamina X are sources of a spino-supraspinal pathway to nucleus accumbens …

Angiotensin Converting Enzyme Activity In Theserum, Lung, Liver And Kidney In Streptozotocin-Induced Diabetic Rats And Diabetic Nephropathy, Bilal Üstündağ, Mehmet Çay, İbrahim H. Özercan, Mustafa Naziroğlu, Necip İlhan

Turkish Journal of Medical Sciences

To clarify the relationship between the alterations of the levels of angiotensin converting enzyme (ACE) and diabetic nephropathy, ACE activity in the lung, liver, kid-ney and serum were investigated in streptozotocin (STZ)-induced diabetic rats. The levels of serum ACE activity unchanged 3 days post STZ treatment but it was significantly an increase 12 and 30 days post STZ treatment in diabetic rats (p

Pulmonary Effects Of Dexfenfluramine, Ferhan Candan, Melih Kaptanoğlu, Reyhan Egi̇lmez, Kasim Doğan, Metin Aksoy, Yücel Yalman, Ebuzer Bekar, Füsun Gülteki̇n

Turkish Journal of Medical Sciences

Dexfenfluramine is a variation of fenfluramine which is used for weight reduction. The aim of the study is to determine the hemodynamic and structural effects of this drug on pulmonary circulation. An experimintal study was carried out with New Zealand Rabbits. A control group (n=30) and an experimintal group (n=20) was constituted. A four-week normal diet for control group and dexfenfluramine plus normala diet for experimental group were applied. Body weights and pulmonary artery pressures of groups were recorded and lungs were collected for pathologic examination. In experimintal group; Dexfenfluramine raised systolic pulmonary artery pressures significantly (p0.05). Weight reduction was …

The Effect Of Diclofenac Sodium On The Renalparenchyma During Complete Unilateral Ureteralobstruction Of The Rats, Cüneyt Turan, Olgun Kontaş, Ahmet Bekereci̇oğlu, Canan Kocaoğlu, Murat Alper, Mustafa Küçükaydin

Turkish Journal of Medical Sciences

An experimental study was designed to investigate the effect of diclofenac sodium (DS) on both renal parenchyma during complete unilateral ureteral obstruction. In 40 adult rats, left ureteropelvic junction were completely obstructed. Rats were divided into 4 equal groups and treated with cefoperazone (Group 2), DS (Group 3), cefoperazone and DS (Group 4) for one week postoperatively. No drug was given to Group 1 (control). Then, both kidneys of all rats were removed and examined histopathologically. The parenchymal thickness of left kid-neys were less than those of rights in all groups. Renal capsuli were moderately thickened in all obstructed kidneys. …

Tumor Necrosis Factor-\Alpha And Interleukin-6 Inperitoneal Adhesion Formation, Mahmut Başoğlu, Ahmet Kiziltunç, Fatih Akçay, Sait Keleş, Cemal Gündoğdu, Durkaya Ören

Turkish Journal of Medical Sciences

This study was carried out to assess the role of tumor necrosis factor (TNF-\alpha) and interleukin-6 (IL-6 ) in plasma and peritoneal fluid in higher rates of adhesion formation following standard bowel injury. Forty-five albino rabbits were divided into three equal groups. Blood was obtained from all the rabbits preoperatively. All the rabbits were subjected to a laparotomy. In group 1(control group), the peritoneal cavity was irrigated with normal saline. In group 2 the cecal serosa was abraded, while the rabbits in group 3 had a resection in small bowel of 2 cm. A peritoneal catheter was placed in all …

Increased Serum Ca 72-4 Levels In Patientswith Gastrointestinal Carcinoma, Mahmut Başoğlu, Ahmet Kiziltunç, Fatih Akçay, Cemal Gündoğdu, İbrahim Kavak, Durkaya Ören

Turkish Journal of Medical Sciences

This study is an evaluation of serum levels in the tumor markers CA 19-9 and CA 72-4 in gastric cancer patients in preoperative, postoperative and follow-up periods. These tumor markers were measured in 35 patients with primary gastric carcinoma (28 advanced stage, 7 early stage), 27 with benign gastric disease and 20 healthy control subjects. Serum CA 72-4 levels were found to have increased (>6.7kU/l) in 16 (14 with advanced stage, 2 with early stage) patients with primary gastric carcinoma. Serum CA 19-9 levels were elevated (>22kU/l) in 9 patients with primary gastric carcinoma (8 with advanced stage, …

Clinical Utility Of Lymphocyte Cross-Matchingafter Renal Transplantation, Olcay Yeği̇n, Gültekin Süleymanlar, Nezih Oygür, Mesut Coşkun, Ayşe Savaş, Nilgün Keçeci̇oğlu, Tuncer Karpuzoğlu

Turkish Journal of Medical Sciences

One of the most important obstacles in renal transplantation is the rejection phenomenon. In spite of the developments in medical technology and immunology, early diagnosis of rejection and specific immunosuppression of the immune attack against the graft have not yet been totally accomplished. Lymphocyte cross-matching (LCM), a test to detect the presence of preformed antibodies in the donor against the recipient, is a practical and cheap method and is performed at almost all renal transplantation centers before transplantation. In this study, we searched for the relation between the posttransplant LCM status and graft survival. LCM was positive in 7 patients …

Glycoprotein Iib/Iiia Inhibitors In Acute Coronarysyndromes: A New Paradigm In Cardiology, Mehmet Ağirbaşli, David M. Kerins

Turkish Journal of Medical Sciences

No abstract provided.

Treatment Of The Multiple Small Stone Particles(Steinstrasse) In The Lover Ureter After Theextracorporeal Shockwave Lithotripsy (Eswl)Treatment, Nihat Satar, Şaban Doran, Reha Özkeçeli̇, R. K. Türkyilmaz

Turkish Journal of Medical Sciences

ESWL is an effective and noninvasive method of treatment for patients with urinary stones. However, especially in the treatment of larger stones impact of the small stone particles in the lower ureter (steinstrasse) is a serious complication that affects the result of the procedure. If the renal unit has not been catheterized before the ESWL procedure, removal of these particles by open surgical or endoscopic intervention within a very short time is mandatory. A total of 1576 renal unit were treated by ESWL with the Dornier MPL 9000 lithotripter from April 1991 to April 1995. Steinstrasse was observved in 81 …