Medicine and Health Sciences Commons^™

Sustained Aurora Kinase B Expression Confers Resistance To Pi3k Inhibition In Head And Neck Squamous Cell Carcinoma, Pooja A Shah, Vaishnavi Sambandam, Anne M Fernandez, Hongyun Zhao, Tuhina Mazumdar, Li Shen, Qi Wang, Kazi M Ahmed, Soma Ghosh, Mitchell J Frederick, Jing Wang, Faye M Johnson

Student and Faculty Publications

UNLABELLED: Tumor suppressor mutations in head and neck squamous cell carcinoma (HNSCC) dominate the genomic landscape, hindering the development of effective targeted therapies. Truncating and missense mutations in NOTCH1 are frequent in HNSCC, and inhibition of PI3K can selectively target NOTCH1 mutant (NOTCH1MUT) HNSCC cells. In this study, we identify several proteins that are differentially regulated in HNSCC cells after PI3K inhibition based on NOTCH1MUT status. Expression of Aurora kinase B (Aurora B), AKT, and PDK1 following PI3K inhibition was significantly lower in NOTCH1MUT cell lines than in wild-type NOTCH1 (NOTCH1WT) cells or NOTCH1MUT cells with acquired resistance to PI3K …

Treatment Of Epilepsy Using A Targeted P38Γ Kinase Gene Therapy, Nicolle Morey, Magdalena Przybyla, Julia Van Der Hoven, Yazi D Ke, Fabien Delerue, Janet Van Eersel, Lars M Ittner

Student and Faculty Publications

Hyperphosphorylated microtubule-associated protein tau has been implicated in dementia, epilepsy, and other neurological disorders. In contrast, site-specific phosphorylation of tau at threonine 205 (T205) by the kinase p38γ was shown to disengage tau from toxic pathways, serving a neuroprotective function in Alzheimer's disease. Using a viral-mediated gene delivery approach in different mouse models of epilepsy, we show that p38γ activity-enhancing treatment reduces seizure susceptibility, restores neuronal firing patterns, reduces behavioral deficits, and ameliorates epilepsy-induced deaths. Furthermore, we show that p38γ-mediated phosphorylation of tau at T205 is essential for this protection in epilepsy, as a lack of this critical interaction reinstates …

A Human Oral Fluid Assay For D- And L- Isomer Detection Of Amphetamine And Methamphetamine Using Liquid-Liquid Extraction, Brian Robbins, Rob E. Carpenter, Mary Long, Jacob Perry

Human Resource Development Faculty Publications and Presentations

Medical providers are increasingly confronted with clinical decision-making that involves (meth)amphetamines. And clinical laboratories need a sensitive, efficient assay for routine assessment of D- and L-isomers to determine the probable source of these potentially illicit analytes. This paper presents a validated method of D- and L-isomer detection in human oral fluid from an extract used for determination of a large oral fluid assay (63 analytes) on an older AB SCIEX 4000 instrument. Taken from the positive extract, D- and L-analytes were added. The method for extraction included addition of internal standard and a 2-step …

Taxonomic Assessment Of Two Wild House Mouse Subspecies Using Whole-Genome Sequencing., Raman Akinyanju Lawal, Verity L Mathis, Mary Barter, Jeremy R. Charette, Alexis Garretson, Beth L Dumont

Faculty Research 2022

The house mouse species complex (Mus musculus) is comprised of three primary subspecies. A large number of secondary subspecies have also been suggested on the basis of divergent morphology and molecular variation at limited numbers of markers. While the phylogenetic relationships among the primary M. musculus subspecies are well-defined, relationships among secondary subspecies and between secondary and primary subspecies remain less clear. Here, we integrate de novo genome sequencing of museum-stored specimens of house mice from one secondary subspecies (M. m. bactrianus) and publicly available genome sequences of house mice previously characterized as M. m. helgolandicus, with whole genome sequences …

Natural Coevolution Of Tumor And Immunoenvironment In Glioblastoma., Lingxiang Wu, Wei Wu, Junxia Zhang, Zheng Zhao, Liangyu Li, Mengyan Zhu, Min Wu, Fan Wu, Fengqi Zhou, Yuxin Du, Rui-Chao Chai, Wei Zhang, Xiaoguang Qiu, Quanzhong Liu, Ziyu Wang, Jie Li, Kening Li, Apeng Chen, Yinan Jiang, Xiangwei Xiao, Han Zou, Rashmi Srivastava, Tingting Zhang, Yun Cai, Yuan Liang, Bin Huang, Ruohan Zhang, Fan Lin, Lang Hu, Xiuxing Wang, Xu Qian, Sali Lv, Baoli Hu, Siyuan Zheng, Zhibin Hu, Hongbing Shen, Yongping You, Roel G W Verhaak, Tao Jiang, Qianghu Wang

Faculty Research 2022

Isocitrate dehydrogenase (IDH) wild-type glioblastoma (GBM) has a dismal prognosis. A better understanding of tumor evolution holds the key to developing more effective treatment. Here we study GBM's natural evolutionary trajectory by using rare multifocal samples. We sequenced 61,062 single cells from eight multifocal IDH wild-type primary GBMs and defined a natural evolution signature (NES) of the tumor. We show that the NES significantly associates with the activation of transcription factors that regulate brain development, including MYBL2 and FOSL2. Hypoxia is involved in inducing NES transition potentially via activation of the HIF1A-FOSL2 axis. High-NES tumor cells could recruit and polarize …

Distinct Tumor Necrosis Factor Alpha Receptors Dictate Stem Cell Fitness Versus Lineage Output In Dnmt3a-Mutant Clonal Hematopoiesis., Jennifer M. Sanmiguel, Elizabeth Eudy, Matthew A. Loberg, Kira Young, Jayna J. Mistry, Kristina D. Mujica, Logan S. Schwartz, Timothy M. Stearns, Grant A Challen, Jennifer J. Trowbridge

Faculty Research 2022

Clonal hematopoiesis resulting from the enhanced fitness of mutant hematopoietic stem cells (HSC) associates with both favorable and unfavorable health outcomes related to the types of mature mutant blood cells produced, but how this lineage output is regulated is unclear. Using a mouse model of a clonal hematopoiesis-associated mutation, DNMT3AR882/+ (Dnmt3aR878H/+), we found that aging-induced TNFα signaling promoted the selective advantage of mutant HSCs and stimulated the production of mutant B lymphoid cells. The genetic loss of the TNFα receptor TNFR1 ablated the selective advantage of mutant HSCs without altering their lineage output, whereas the loss of TNFR2 resulted in …

Promoting Sleep To Decrease Delirium, Stephanie Mayne

MSN Capstone Projects

Delirium is potent risk factor for complications, an increase length of stay, and mortality. Hospital acquired delirium is a prevalent and serious health condition that can occur in patients, especially the older adult population, when admitted to the hospital. It has the ability to be prevented if properly addressed and interventions are set in place to help reduce the occurrence of hospital acquired delirium. Many factors can contribute to the development of delirium, including a disruption of one’s circadian rhythm. Sleep is not always a high priority in the hospital, therefore there are little interventions and goals aimed at improving …

Association Of Maternal Age 35 Years And Over And Prenatal Care Utilization, Preterm Birth, And Low Birth Weight, Mexico 2008–2019, Laura Jacobson, Evelyn Fuentes-Rivera, Raffaela Schiavon, Blair Darney

OHSU-PSU School of Public Health Faculty Publications and Presentations

Objective:

We compared prenatal care utilization, preterm birth, and low birth weight neonates among women 35 years and older compared to women 20-34 years old in Mexico, 2008-2019.

Methods:

We used birth certificate data and conducted a historical cohort study of all singleton live births in Mexico from 2008-2019. Study outcomes were inadequate prenatal care (timing of initiation of care and number of visits), preterm birth, and low birth weight. We compared outcomes among women 35-39, 40-44, and 45-49 with births to women 20-34. We used logistic regression to account for individual and contextual confounders.

Results:

We included a total …

Does The Impact Of Arthritis On Participation In Social Activity Vary By Depression?, Mehnaaz Ali

Master of Public Health Program Student Publications

Objective As the population’s longevity is increasing over time, the number of comorbidities that individuals are suffering with are also becoming evident. The objective is to determine whether individuals living with arthritis have difficulty in social activity participation while suffering with depression. Using the 2019 National Health Interview Survey, this analysis included males and females between 18 to 85 years of age. The dependent variable was social activity participation, where it was identifying participants whether they are or are not having difficulty taking part in social activities. The primary independent variables were depression and arthritis. Other covariates included the demographics …

Regional Anesthesia In Outpatient Total Joint Arthroplasty, Laci Caesar

MSN Capstone Projects

Within the past five years, outpatient total joint arthroplasties have increased three-fold due to the change in insurance companies’ reimbursement plans. After continuous literature research collection, support for spinal anesthesia was higher than compared to general anesthesia (GA) for outpatient total joint arthroplasty procedures. Healthy patient who receives regional anesthesia showed decreased post-operative pain, increased rehabilitation, and, most of all, a decrease in facility stays (Macfarlane et al., 2009). This has allowed healthy patients who meet physician, anesthesia, and facility requirements to transition from hospitals to ambulatory surgery centers for total joint replacement procedures. With this new transition comes change …

Suicide Among South Asians In The United States: A Growing Public Health Problem, Aruna Jha, Manik Ahuja, Rajvi J. Wani

ETSU Faculty Works

This research study quantifies and describes suicide among South Asian Americans (SAAs), an emerging population that are underrepresented group in suicide research. The purpose of this study was to examine key characteristics of suicide deaths among SAAs. Data were employed from DuPage County, IL, a county with a large SAA population. Following federal recommendations for disaggregating Asian American data at a granular level, four SAA researchers used name recognition to identify all SAA cases classified as suicide in the DuPage County coroner’s database from 2001 to 2017 (N = 38). Coroner’s reports were analyzed for contextual details and correlating …

Human Papillomavirus Vaccine Administration Trends Among Commercially Insured Us Adults Aged 27-45 Years Before And After Advisory Committee On Immunization Practices Recommendation Change, 2007-2020, Ryan Suk, Kaiping Liao, Cici X Bauer, Catherine Basil, Meng Li

Student and Faculty Publications

IMPORTANCE: In 2019, the Advisory Committee on Immunization Practices (ACIP) recommended patient-clinician shared decision-making for human papillomavirus (HPV) vaccination in adults aged 27 to 45 years. Less is known about the HPV vaccine administration trends in this age group before and after this recommendation update.

OBJECTIVE: to examine the association between the ACIP recommendation update and the HPV vaccine administration among US adults aged 27 to 45 years.

DESIGN, SETTING, AND PARTICIPANTS: This large commercial claim-based retrospective cohort study used the Optum Clinformatics database for validated claims from January 1, 2007, through December 31, 2020. A total of 22 600 …

Distinct Tumor Necrosis Factor Alpha Receptors Dictate Stem Cell Fitness Versus Lineage Output In Dnmt3a-Mutant Clonal Hematopoiesis, Jennifer M Sanmiguel, Elizabeth Eudy, Matthew A Loberg, Kira A Young, Jayna J Mistry, Kristina D Mujica, Logan S Schwartz, Timothy M Stearns, Grant A Challen, Jennifer J Trowbridge

2020-Current year OA Pubs

UNLABELLED: Clonal hematopoiesis resulting from the enhanced fitness of mutant hematopoietic stem cells (HSC) associates with both favorable and unfavorable health outcomes related to the types of mature mutant blood cells produced, but how this lineage output is regulated is unclear. Using a mouse model of a clonal hematopoiesis-associated mutation, DNMT3AR882/+ (Dnmt3aR878H/+), we found that aging-induced TNFα signaling promoted the selective advantage of mutant HSCs and stimulated the production of mutant B lymphoid cells. The genetic loss of the TNFα receptor TNFR1 ablated the selective advantage of mutant HSCs without altering their lineage output, whereas the loss of TNFR2 resulted …

Identification Of Novel Antifungal Skeleton Of Hydroxyethyl Naphthalimides With Synergistic Potential For Chemical And Dynamic Treatments, Pengli Zhang, Vijai Kumar Reddy Tangadanchu, Chenghe Zhou

2020-Current year OA Pubs

The invasion of pathogenic fungi poses nonnegligible threats to the human health and agricultural industry. This work exploited a family of hydroxyethyl naphthalimides as novel antifungal species with synergistic potential of chemical and dynamic treatment to combat the fungal resistance. These prepared naphthalimides showed better antifungal potency than fluconazole towards some tested fungi including

Genome-Edited Zebrafish Model Of Abcc8 Loss-Of-Function Disease, Jennifer M Ikle, Robert C Tryon, Soma S Singareddy, Nathaniel W York, Maria S Remedi, Colin G Nichols

2020-Current year OA Pubs

ATP-sensitive potassium channel (K

Hyperkalemia And Electrocardiogram Manifestations In End-Stage Renal Disease, Zubaid Rafique, Bryan Hoang, Heba Mesbah, Ryan Pappal, Frank W Peacock, Raul Juarez-Vela, Lukasz Szarpak, Dick C Kuo

2020-Current year OA Pubs

Hyperkalemia is one of the more common acute life-threatening metabolic emergencies. The aim of our study is to determine the correlation and accuracy of abnormal ECG parameters as a function of serum potassium concentration in the end-stage renal disease (ESRD) population. We performed a retrospective chart review of emergency department patients presenting with ESRD and receiving emergent hemodialysis treatment. A total of 96 patients, each with five independent ED visits, provided 480 sets of ECGs and electrolytes. Of these, four ECGs were excluded for inability to interpret, leaving a total of 476 patient encounters that met all inclusion criteria. Linear …

Equity Implications Of Hospital Penalties During 4 Years Of The Comprehensive Care For Joint Replacement Model, 2016 To 2019, Sukruth A Shashikumar, Andrew M Ryan, Karen E Joynt Maddox

2020-Current year OA Pubs

No abstract provided.

Intracranial Carotid Arteriosclerosis Mediates The Association Between Blood Pressure And Cerebral Small Vessel Disease, Jesus D. Melgarejo, Meike W. Vernooij, M. Arfan Ikram, Zhen-Yu Zhang, Daniel Bos

School of Medicine Publications and Presentations

Background:

Intracranial arteriosclerosis could explain the association between blood pressure (BP) and cerebral small vessel disease (CSVD). Therefore, we tested whether intracranial carotid artery calcification (ICAC) mediates the association between BP and CSVD and determined pathophysiological mechanisms based on ICAC subtypes.

Methods:

One thousand four hundred fifty-eight stroke-free participants from the Rotterdam Study (mean age, 68 years; 52% women) underwent nonenhanced computed tomography scans to quantify ICAC volume (mm3) between 2003 and 2015. ICAC was categorized into intimal and internal elastic lamina calcifications. CSVD included white matter hyperintensities volume, the presence of lacunes, and cerebral microbleeds visualized on magnetic resonance …

Epc-Exs Improve Astrocyte Survival And Oxidative Stress Through Different Uptaking Pathways In Diabetic Hypoxia Condition, Manasi Suchit Halurkar, Jinju Wang, Shuzhen Chen, Ji Chen Bihl

Pharmacology and Toxicology Faculty Publications

Background: Hyperglycemia contributes to cardiovascular complications in patients with type 2 diabetes. We confirmed that high glucose (HG) induces endothelial dysfunction and cerebral ischemic injury is enlarged in diabetic mice. Stem cell-released exosomes have been shown to protect the brain from ischemic stroke. We have previously shown that endothelial progenitor cells (EPCs)-released exosomes (EPC-EXs) can protect endothelial cells from hypoxia/reoxygenation (H/R) and HG-induced injury. Here, we aim to investigate the effects of EPC-EXs on astrocytes under H/R and HG-induced injury and whether miR-126 enriched EPC-EXs (miR126-EPC-EXs) have enhanced efficacy. Methods: EPC-EX uptake and co-localization were measured by fluorescent microscopy using …

App-Licable Techology, Wynter Hale, Cierilyn Legaspi, Juan Garcia, Alex Zamora

Occupational Therapy Student Research

Autism Spectrum Disorder (ASD) has seen an increase in prevalence in the United States presenting in 1 in 59 children. ASD presents with an array of symptoms that impact various aspects of life including but not limited to, social interaction, attention, and behavioral modulation (Voss et al., 2019). Meristem is a transitional program for individuals ages 18-28 that prepares young adults on the transition of adulthood while coping with the various symptoms of ASD. At our fieldwork site, our collective mission has been to focus on sensory and mood self-regulation to improve the individual's independence during situations. Technology can play …

Point Of View Video Modeling For Improved Self Care Among Asd Population, Liana Mae Atizado, Cassey Dela Pena, Anali Flores, Jeffrey Garcia, Timothy Zeng

Occupational Therapy Student Research

Meristem is a program designed for young adults with autism spectrum disorder (ASD) that emphasizes teaching the necessary skills for students to live life independently with confidence. Through structured and semi-structured interviews with staff members and students, a need that was identified within this organization is to have a thorough and consistent personal hygiene regimen for their students. While Meristem is currently implementing standard personal hygiene care in their curriculum, the variability of students understanding and implementing personal hygiene education has become an obstacle within this organization. To address this need for individuals with ASD, Video Modeling (VM) can offer …

Beyond Verbal: A Low-Cost Communication Tool To Address Language Barriers In A Community-Based Setting, Nikki Atis, Raman Dhillon, Victoria Maichel, Gabe Soto, Brenda Vergara

Occupational Therapy Student Research

Augmentative and Alternative Communication (AAC) are tools, technologies, and approaches used to provide ways other than speech to aid communication-related challenges (American Speech-Language-Hearing Association, n.d.). A communication board is a low-tech AAC tool seen in various formats such as printed on a poster board or digital applications on electronic devices. It consists of rows and columns of squares with simple pictures and minimally-worded labels in the preferred language.

Kitchen Stories, Terral Christopher, Nazifa Fazil, Lauren Jew, Ken Zhu

Occupational Therapy Student Research

Individuals with ASD may experience difficulties with independent living skills, such as financial management, home management and meal preparation. An occupation such as cooking typically includes methods such as task-analysis, systemic prompting, cueing, modeling, visual support and feedback, which can be challenging for the ASD population.

Kitchen Stories is a video-based, cross-media cooking platform whose mission is to “to empower people to cook a fresh meal every day.” Although cooking is an essential part of everyday life, the creators of the platform understand that many people struggle with it for a variety of reasons. Nevertheless, they are confident that anyone …

Jackery Power Station: Stockton Shelter For The Homeless, Angela Vides, Abigail Equinoa, Carolyn Ly, Estephanie Huerta

Occupational Therapy Student Research

The community group, San Joaquin County Mobile Clinic, aims to improve the health outcomes of vulnerable populations by providing low-cost preventative healthcare and treatment options. This includes wound care, vaccinations, and assistance with accessing medical appointments and health insurance. However, research has shown that those without housing do not have adequate access to stable power sources to charge mobile devices to aid healthcare needs and access resources. The Jackery Power Station, which falls under the category of consumer access, priorities, and benefits over the lifespan, will attempt to address this barrier.

An estimated 2,631 individuals in San Joaquin county are …

Adaptive Seating, Julie Jones, Mckayla Wallace, Marisa Given, Nisha Rustia, George Cardenas

Occupational Therapy Student Research

Children’s sensory needs are impacting their ability to attend to their speech language pathology treatment sessions in a meaningful and effective way. There is a gap in the education for SLPs on sensory integration which limits their ability to recognize the challenges and know how to support them in a productive way.

Weighted Devices For Somatosensory Regulation, Bri Swain, Kaelyn Obryan, Lauren Magat, Nik Gargiulo, Hailey Houck

Occupational Therapy Student Research

Description: A weighted vest is a vest that typically has 10% of a person’s body weight evenly distributed around the vest (Olson et al., 2004). A weighted vest can provide sensory input that is believed to alleviate dysregulation, inattentiveness, and stereotypic behaviors (Carter et al., 2009).

Goal: Our community group is specifically targeting children with sensory processing impairments who are attending speech therapy sessions at the RiteCare clinic. Sensory behaviors exhibited by the clients are most commonly shown through dysregulation, outbursts, inattentiveness, and hyperactivity. The goal is to limit these maladaptive behaviors and increase attention span during intervention sessions. Weighted …

Insight Timer, Jocelyn Ong Najera, Joshua Mark Dano, Staphany Jimenez, Arthur Lui, Nancy Smith

Occupational Therapy Student Research

Mission Statement: To provide cost-effective avenues for tangible stress reduction and relief for low-income communities such as Wellspring.

Overview: Insight Timer is a mobile application that offers guided meditation to calm the mind, reduce anxiety, manage stress, sleep deeply, and improve happiness. While also offering calm music, courses, live events, and workshops in various languages. (Insight Network, Inc., n.d.)

Problem Statement: Individuals who face poverty experience mental challenges due to stressors caused by poverty due to insecurity and uncertainty about food, housing, and income. Low-income communities tend to have limited resources, poor housing, experience high crime rates, violence rate, and …

Fetal Sex Impacts Birth To Placental Weight Ratio And Umbilical Cord Oxygen Values With Implications For Regulatory Mechanisms, Bryan S. Richardson, Akasham Rajagopaul, Barbra De Vrijer, Genevieve Eastabrook, Timothy R.H. Regnault

Obstetrics & Gynaecology Publications

Background: We determined the effect of fetal sex on birth/placental weight and umbilical vein and artery oxygen values with implications for placental efficiency and regulatory mechanisms underlying fetal–placental growth differences. Methods: A hospital database was used to obtain birth/placental weight, cord PO2 and other information on patients delivering between Jan 1, 1990 and Jun 15, 2011 with GA > 34 weeks (N = 69,836). Oxygen saturation was calculated from the cord PO2 and pH data, while fractional O2 extraction was calculated from the oxygen saturation data. The effect of fetal sex on birth/placental weight, cord PO2, O2 saturation, and fractional O2 …

Expression And Localization Of Nrf2/Keap1 Signalling Pathway Genes In Mouse Preimplantation Embryos Exposed To Free Fatty Acids., Grace Dionne, Michele D. Calder, Dean H Betts, Basim Abu Rafea, Andrew J Watson

Obstetrics & Gynaecology Publications

Obese women experience greater incidence of infertility, with reproductive tracts exposing preimplantation embryos to elevated free fatty acids (FFA) such as palmitic acid (PA) and oleic acid (OA). PA treatment impairs mouse preimplantation development in vitro, while OA co-treatment rescues blastocyst development of PA treated embryos. In the present study, we investigated the effects of PA and OA treatment on NRF2/Keap1 localization, and relative antioxidant enzyme (Glutathione peroxidase; Gpx1, Catalase; Cat, Superoxide dismutase; Sod1 and γ-Glutamylcysteine ligase catalytic unit; Gclc) mRNA levels, during in vitro mouse preimplantation embryo development. Female mice were superovulated, mated, and embryos cultured in the presence …

Assessing Cerebral Blood Flow, Oxygenation And Cytochrome C Oxidase Stability In Preterm Infants During The First 3 Days After Birth, Ajay Rajaram, Daniel Milej, Marianne Suwalski, Lilian Kebaya, Matthew Kewin, Lawrence Yip, Sandrine De Ribaupierre, Victor Han, Mamadou Diop, Soume Bhattacharya, Keith St. Lawrence

Paediatrics Publications

A major concern with preterm birth is the risk of neurodevelopmental disability. Poor cerebral circulation leading to periods of hypoxia is believed to play a significant role in the etiology of preterm brain injury, with the first three days of life considered the period when the brain is most vulnerable. This study focused on monitoring cerebral perfusion and metabolism during the first 72 h after birth in preterm infants weighing less than 1500 g. Brain monitoring was performed by combining hyperspectral near-infrared spectroscopy to assess oxygen saturation and the oxidation state of cytochrome c oxidase (oxCCO), with diffuse correlation spectroscopy …