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Articles 1 - 30 of 95
Full-Text Articles in Medicine and Health Sciences
Impact Of Forearm Pronation On Ultrasound-Measured Forearm Muscle Thickness In Children And Adolescents, Takashi Abe, Hayao Ozaki, Akemi Abe, Jeremy P. Loenneke
Impact Of Forearm Pronation On Ultrasound-Measured Forearm Muscle Thickness In Children And Adolescents, Takashi Abe, Hayao Ozaki, Akemi Abe, Jeremy P. Loenneke
Faculty and Student Publications
Background and Aim: It was unknown whether ultrasound-measured forearm muscle thickness was impacted by pronation of the forearm. The aim of this study was to investigate the influence of forearm pronation on two forearm muscle thicknesses (MT-ulna and MT-radius). Participants and Methods: Fourteen healthy children and adolescents sat on a chair with their right arm comfortably on a table, and their hands were fixed to the board with elastic bands. The probe was placed perpendicularly over the forearm, and the angle of the board was then pronated in 5° increments from -10° to 30°. The average value of the two …
Diverse Glycosides From Gardenia Latifolia With Antiviral Activity And Chemosystematic Significance, Shaymaa M. Mohamed, Samir A. Ross, Mai A. M. Ahmed
Diverse Glycosides From Gardenia Latifolia With Antiviral Activity And Chemosystematic Significance, Shaymaa M. Mohamed, Samir A. Ross, Mai A. M. Ahmed
Faculty and Student Publications
Several influenza pandemics have impacted our life, each with variable prevalence and severity. In a search for natural antivirals, further phytochemical investigation of Gardenia latifolia Aiton, Rubiaceae, was conducted. As a result, five structurally diverse glycosides were isolated, offering valuable chemotaxonomic data. Using the crystal violet technique, three isolates, canthoside C, (6R,7S,8S)-7α-[(β-d-glucopyranosyl) oxy] lyoniresinol, and ecdysanrosin A, were evaluated for their anti-influenza A (H1N1) activities. Based on previously reported anti-inflammatory activity of the guaiane class, we investigated the inhibitory effect of (1R,7R,8S,10R)-7,8,11-trihydroxy-guai-4-ene-3-one 8-O-β-d-glucopyranoside, a rare guaiane sesquiterpene glucoside, on inducible nitric oxide (NO) production by Griess assay. Regarding antiviral assay, …
Tlr4/Nfκb-Mediated Anti-Inflammatory And Antioxidative Effect Of Hexanic And Ethanolic Extracts Of Curcuma Longa L. In Buffalo Mammary Epithelial Cells, Insha Amin, Shahzada Mudasir Rashid, Sheikh Shubeena, Ishraq Hussain
Tlr4/Nfκb-Mediated Anti-Inflammatory And Antioxidative Effect Of Hexanic And Ethanolic Extracts Of Curcuma Longa L. In Buffalo Mammary Epithelial Cells, Insha Amin, Shahzada Mudasir Rashid, Sheikh Shubeena, Ishraq Hussain
Faculty and Student Publications
Mastitis is the commonest disease of bovines imposing a great economic setback and a drastic public health concern worldwide. Antibiotic resistance is the preliminary con of excessive antibiotics use for disease treatment. Studies validate an alarming increase in the antibiotic resistance of both humans and animals. For ages, medicinal plants have been used to treat a number of ailments in humans and animals, including mastitis. Curcuma longa, or turmeric, is the prerequisite in Indian cooking and has been used in traditional medicine for its magical properties. The rhizome of C. longa possesses significant anti-inflammatory, antibacterial, antifungal, and antioxidative properties. In …
Immunogenetic Role Of Il17a Polymorphism In The Pathogenesis Of Recurrent Miscarriage, Shafat Ali, Sabhiya Majid, Md Niamat Ali, Mujeeb Zafar Banday
Immunogenetic Role Of Il17a Polymorphism In The Pathogenesis Of Recurrent Miscarriage, Shafat Ali, Sabhiya Majid, Md Niamat Ali, Mujeeb Zafar Banday
Faculty and Student Publications
Interleukin-17A (IL17A) is a proinflammatory cytokine and is assumed to play an important role in fetal rejection. In order to evaluate the potential role of IL17A polymorphism in the pathogenesis of recurrent miscarriage (RM), serum IL17A levels were estimated by ELISA. Single-nucleotide polymorphism was assessed by polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) using gene-specific primers and the EcoNI restriction enzyme. Serum IL17A levels were nonsignificantly (p > 0.5) low in RM patients compared with the control group. IL17A gene amplification by PCR yielded the undigested product of 815 bp, and its digestion with EcoNI enzyme produced 815, 529, 286, and 270 …
Antitrypanosomal, Antitopoisomerase-I, And Cytotoxic Biological Evaluation Of Some African Plants Belonging To Crassulaceae; Chemical Profiling Of Extract Using Uhplc/Qtof-Ms/Ms, Mostafa M. Hegazy, Wael M. Afifi, Ahmed M. Metwaly, Mohamed M. Radwan
Antitrypanosomal, Antitopoisomerase-I, And Cytotoxic Biological Evaluation Of Some African Plants Belonging To Crassulaceae; Chemical Profiling Of Extract Using Uhplc/Qtof-Ms/Ms, Mostafa M. Hegazy, Wael M. Afifi, Ahmed M. Metwaly, Mohamed M. Radwan
Faculty and Student Publications
In our continuous study for some African plants as a source for antitrypanosomally and cytotoxic active drugs, nine different plants belonging to the Crassulaceae family have been selected for the present study. Sedum sieboldii leaves extract showed an antitrypanosomal activity against Trypanosoma brucei with an IC50 value of 8.5 µg/mL. In addition, they have cytotoxic activities against (HCT-116), (HEPG-2) and (MCF-7), with IC50 values of 28.18 ± 0.24, 22.05 ± 0.66, and 26.47 ± 0.85 µg/mL, respectively. Furthermore, the extract displayed inhibition against Topoisomerase-1 with an IC50 value of 1.31 µg/mL. It showed the highest phenolics and flavonoids content among …
Isolation And Characterization Of Flavonoid Naringenin And Evaluation Of Cytotoxic And Biological Efficacy Of Water Lilly (Nymphaea Mexicana Zucc.), Shajrath Din, Saima Hamid, Aadil Yaseen, Ali Mohd Yatoo
Isolation And Characterization Of Flavonoid Naringenin And Evaluation Of Cytotoxic And Biological Efficacy Of Water Lilly (Nymphaea Mexicana Zucc.), Shajrath Din, Saima Hamid, Aadil Yaseen, Ali Mohd Yatoo
Faculty and Student Publications
Despite its limited exploration, Nymphaea mexicana Zucc. can be beneficial if pharmacology, isolation, and biological evaluation are given attention. It is an aquatic species that belongs to the family Nymphaeaceae. The thrust area of the work was the extraction, isolation, and biological evaluation of different extracts of the N. mexicana Zucc. plant. The primary goal of this research was to assess the antimicrobial, antioxidant, and anticancer activities of the extracts and to isolate the target naringenin compound. Comparative FT IR analysis of different extracts of this plant revealed the presence of functional groups of plant secondary metabolites, including polyphenols, flavonoids, …
Bioassay-Guided Fractionation With Antimalarial And Antimicrobial Activities Of Paeonia Officinalis, Mamdouh Nabil Samy, Basma Khalaf Mahmoud, Nourhan Hisham Shady, Usama Ramadan Abdelmohsen
Bioassay-Guided Fractionation With Antimalarial And Antimicrobial Activities Of Paeonia Officinalis, Mamdouh Nabil Samy, Basma Khalaf Mahmoud, Nourhan Hisham Shady, Usama Ramadan Abdelmohsen
Faculty and Student Publications
Bioassay-guided fractionation technique of roots of Paeonia officinalis led to isolation and structure elucidation of seven known compounds, including four monoterpene glycosides: lactiflorin (1), paeoniflorin (4), galloyl paeoniflorin (5), and (Z)-(1S,5R)-β-pinen-10-yl β-vicianoside (7); two phenolics: benzoic acid (2) and methyl gallate (3); and one sterol glycoside: β-sitosterol 3-O-β-D-glucopyranoside (6). The different fractions and the isolated compounds were evaluated for their antimicrobial and antimalarial activities. Fraction II and III showed antifungal activity against Candida neoformans with IC50 values of 28.11 and 74.37 µg/mL, respectively, compared with the standard fluconazole (IC50 = 4.68 µg/mL), and antibacterial potential against Pseudomonas aeruginosa (IC50 = …
Cannabidiol Loaded Topical Ophthalmic Nanoemulsion Lowers Intraocular Pressure In Normotensive Dutch-Belted Rabbits, Samir Senapati, Ahmed Adel Ali Youssef, Corinne Sweeney, Chuntian Cai
Cannabidiol Loaded Topical Ophthalmic Nanoemulsion Lowers Intraocular Pressure In Normotensive Dutch-Belted Rabbits, Samir Senapati, Ahmed Adel Ali Youssef, Corinne Sweeney, Chuntian Cai
Faculty and Student Publications
Cannabidiol (CBD) is the major non-psychoactive and most widely studied of the cannabinoid constituents and has great therapeutic potential in a variety of diseases. However, contradictory reports in the literature with respect to CBD’s effect on intraocular pressure (IOP) have raised concerns and halted research exploring its use in ocular therapeutics. Therefore, the current investigation aimed to further evaluate CBD’s impact on the IOP in the rabbit model. CBD nanoemulsions, containing Carbopol® 940 NF as a mucoadhesive agent (CBD-NEC), were prepared using hot-homogenization followed by probe sonication. The stability of the formulations post-moist-heat sterilization, in terms of physical and chemical …
Abcg2 Polymorphisms And Predictive Fluoroquinolone Phototoxicity In Nondomestic Felids, Alexandria E. Gochenauer, Dayna L. Dreger, Brian W. Davis, Shawna Cook
Abcg2 Polymorphisms And Predictive Fluoroquinolone Phototoxicity In Nondomestic Felids, Alexandria E. Gochenauer, Dayna L. Dreger, Brian W. Davis, Shawna Cook
Faculty and Student Publications
Fluoroquinolones are a widely used class of chemotherapeutics within veterinary medicine, prized for their broad-spectrum bactericidal activity. These drugs present a known risk of retinal phototoxicity in domestic cats (Felis catus); therefore, using lower doses and alternative antibiotic classes is encouraged in this species. This adverse drug effect of fluoroquinolones, and enrofloxacin specifically, has been determined to be species-specific in domestic felids. Four feline-specific missense variants in ABCG2 result in four amino acid changes (E159M, S279L, H283Q, and T644I) that are unique to the domestic cat compared with multiple other nonfeline mammalian species. These changes alter the ABCG2 protein involved …
Understanding The Dynamics Of The Structural States Of Cannabinoid Receptors And The Role Of Different Modulators, Anjela Manandhar, Mona H. Haron, Michael L. Klein, Khaled Elokely
Understanding The Dynamics Of The Structural States Of Cannabinoid Receptors And The Role Of Different Modulators, Anjela Manandhar, Mona H. Haron, Michael L. Klein, Khaled Elokely
Faculty and Student Publications
The cannabinoid receptors CB1R and CB2R are members of the G protein-coupled receptor (GPCR) family. These receptors have recently come to light as possible therapeutic targets for conditions affecting the central nervous system. However, because CB1R is known to have psychoactive side effects, its potential as a drug target is constrained. Therefore, targeting CB2R has become the primary focus of recent research. Using various molecular modeling studies, we analyzed the active, inactive, and intermediate states of both CBRs in this study. We conducted in-depth research on the binding properties of various groups of cannabinoid modulators, including agonists, antagonists, and inverse …
How Carbon Nanoparticles, Arbuscular Mycorrhiza, And Compost Mitigate Drought Stress In Maize Plant: A Growth And Biochemical Study, Emad A. Alsherif, Omar Almaghrabi, Ahmed M. Elazzazy, Mohamed Abdel-Mawgoud
How Carbon Nanoparticles, Arbuscular Mycorrhiza, And Compost Mitigate Drought Stress In Maize Plant: A Growth And Biochemical Study, Emad A. Alsherif, Omar Almaghrabi, Ahmed M. Elazzazy, Mohamed Abdel-Mawgoud
Faculty and Student Publications
Drought negatively affects crop growth and development, so it is crucial to develop practical ways to reduce these consequences of water scarcity. The effect of the interactive potential of compost (Comp), mycorrhizal fungi (AMF), and carbon nanoparticles (CNPS) on plant growth, photosynthesis rate, primary metabolism, and secondary metabolism was studied as a novel approach to mitigating drought stress in maize plants. Drought stress significantly reduced maize growth and photosynthesis and altered metabolism. Here, the combined treatments Com-AMF or Com-AMF-CNPs mitigated drought-induced reductions in fresh and dry weights. The treatments with AMF or CNPS significantly increased photosynthesis (by 10%) in comparison …
Discovery Of A Lectin Domain That Regulates Enzyme Activity In Mouse N-Acetylglucosaminyltransferase-Iva (Mgat4a), Masamichi Nagae, Tetsuya Hirata, Hiroaki Tateno, Sushil K. Mishra
Discovery Of A Lectin Domain That Regulates Enzyme Activity In Mouse N-Acetylglucosaminyltransferase-Iva (Mgat4a), Masamichi Nagae, Tetsuya Hirata, Hiroaki Tateno, Sushil K. Mishra
Faculty and Student Publications
N-Glycosylation is a common post-translational modification, and the number of GlcNAc branches in N-glycans impacts glycoprotein functions. N-Acetylglucosaminyltransferase-IVa (GnT-IVa, also designated as MGAT4A) forms a β1-4 GlcNAc branch on the α1-3 mannose arm in N-glycans. Downregulation or loss of GnT-IVa causes diabetic phenotypes by dysregulating glucose transporter-2 in pancreatic β-cells. Despite the physiological importance of GnT-IVa, its structure and catalytic mechanism are poorly understood. Here, we identify the lectin domain in mouse GnT-IVa’s C-terminal region. The crystal structure of the lectin domain shows structural similarity to a bacterial GlcNAc-binding lectin. Comprehensive glycan binding assay using 157 glycans and solution NMR …
Milk-Compositional Study Of Metabolites And Pathogens In The Milk Of Bovine Animals Affected With Subclinical Mastitis, Aarif Ali, Manzoor Ur Rahman Mir, Showkat Ahmad Ganie, Saima Mushtaq
Milk-Compositional Study Of Metabolites And Pathogens In The Milk Of Bovine Animals Affected With Subclinical Mastitis, Aarif Ali, Manzoor Ur Rahman Mir, Showkat Ahmad Ganie, Saima Mushtaq
Faculty and Student Publications
Bovine milk is an important food component in the human diet due to its nutrient-rich metabolites. However, bovine subclinical mastitis alters the composition and quality of milk. In present study, California mastitis testing, somatic cell count, pH, and electrical conductivity were used as confirmatory tests to detect subclinical mastitis. The primary goal was to study metabolome and identify major pathogens in cows with subclinical mastitis. In this study, 29 metabolites were detected in milk using gas chromatography–mass spectrometry. Volatile acidic compounds, such as hexanoic acid, hexadecanoic acid, lauric acid, octanoic acid, n-decanoic acid, tricosanoic acid, tetradecanoic acid, and hypogeic acid …
Validated Determination Of Nrg1 Ig-Like Domain Structure By Mass Spectrometry Coupled With Computational Modeling, Niloofar Abolhasani Khaje, Alexander Eletsky, Sarah E. Biehn, Charles K. Mobley
Validated Determination Of Nrg1 Ig-Like Domain Structure By Mass Spectrometry Coupled With Computational Modeling, Niloofar Abolhasani Khaje, Alexander Eletsky, Sarah E. Biehn, Charles K. Mobley
Faculty and Student Publications
High resolution hydroxyl radical protein footprinting (HR-HRPF) is a mass spectrometry-based method that measures the solvent exposure of multiple amino acids in a single experiment, offering constraints for experimentally informed computational modeling. HR-HRPF-based modeling has previously been used to accurately model the structure of proteins of known structure, but the technique has never been used to determine the structure of a protein of unknown structure. Here, we present the use of HR-HRPF-based modeling to determine the structure of the Ig-like domain of NRG1, a protein with no close homolog of known structure. Independent determination of the protein structure by both …
Prescribing Trends Of Proton Pump Inhibitors, Antipsychotics And Benzodiazepines Of Medicare Part D Providers, Jennifer M. Toth, Saumil Jadhav, Holly M. Holmes, Manvi Sharma
Prescribing Trends Of Proton Pump Inhibitors, Antipsychotics And Benzodiazepines Of Medicare Part D Providers, Jennifer M. Toth, Saumil Jadhav, Holly M. Holmes, Manvi Sharma
Faculty and Student Publications
Background: Proton pump inhibitors, benzodiazepines, and antipsychotics are considered potentially inappropriate medications in older adults according to the American Geriatric Society Beers Criteria, and deprescribing algorithms have been developed to guide use of these drug classes. The objective of this study was to describe the number of beneficiaries prescribed these medications, provider specialty and regional trends in prescribing, and the aggregate costs for these claims in Medicare Part D. Methods: This was a retrospective cross-sectional study using publicly available Medicare Provider Utilization and Payment Data: Part D Prescriber data for years 2013–2019. Descriptive statistics and the Cochrane-Armitage test were used …
Utility Of Fatty Acid Profile And In Vitro Immune Cell Activation For Chemical And Biological Standardization Of Arthrospira/Limnospira, Jungmoo Huh, Jin Zhang, Radka Hauerová, Joseph Lee
Utility Of Fatty Acid Profile And In Vitro Immune Cell Activation For Chemical And Biological Standardization Of Arthrospira/Limnospira, Jungmoo Huh, Jin Zhang, Radka Hauerová, Joseph Lee
Faculty and Student Publications
Commercially cultivated Limnospira (species formerly classified to genus Arthrospira) is a popular food/supplement consumed by millions of people worldwide for health benefits. The objective of the current research was to advance the standardization technology for Limnospira. Quantitative methods were established to detect fatty acids as potential chemical markers and immune-enhancing activity. Analysis of 20 different batches of biomass obtained from one commercial grower demonstrated that there was a statistically significant relationship between the sum of two fatty acids (linoleic and γ-linolenic) and Toll-like receptor (TLR)2/TLR1-dependent activation (R2 = 0.48, p = 0.0007). Investigation of 12 biomass samples sourced from growers …
Comparative Pharmacokinetics And Tissue Distribution Of Primaquine Enantiomers In Mice, Pius S. Fasinu, Narayan D. Chaurasiya, N. P. Dhammika Nanayakkara, Yan‑Hong ‑H Wang
Comparative Pharmacokinetics And Tissue Distribution Of Primaquine Enantiomers In Mice, Pius S. Fasinu, Narayan D. Chaurasiya, N. P. Dhammika Nanayakkara, Yan‑Hong ‑H Wang
Faculty and Student Publications
Background: Primaquine (PQ) has been used for the radical cure of relapsing Plasmodium vivax malaria for more than 60 years. PQ is also recommended for prophylaxis and prevention of transmission of Plasmodium falciparum. However, clinical utility of PQ has been limited due to toxicity in individuals with genetic deficiencies in glucose 6-phosphate dehydrogenase (G6PD). PQ is currently approved for clinical use as a racemic mixture. Recent studies in animals as well as humans have established differential pharmacological and toxicological properties of the two enantiomers of PQ. This has been attributed to differential metabolism and pharmacokinetics of individual PQ enantiomers. The …
A Distinct Mechanism Of C-Type Inactivation In The Kv-Like Kcsa Mutant E71v, Ahmed Rohaim, Bram J. A. Vermeulen, Jing Li, Felix Kümmerer
A Distinct Mechanism Of C-Type Inactivation In The Kv-Like Kcsa Mutant E71v, Ahmed Rohaim, Bram J. A. Vermeulen, Jing Li, Felix Kümmerer
Faculty and Student Publications
C-type inactivation is of great physiological importance in voltage-activated K+ channels (Kv), but its structural basis remains unresolved. Knowledge about C-type inactivation has been largely deduced from the bacterial K+ channel KcsA, whose selectivity filter constricts under inactivating conditions. However, the filter is highly sensitive to its molecular environment, which is different in Kv channels than in KcsA. In particular, a glutamic acid residue at position 71 along the pore helix in KcsA is substituted by a valine conserved in most Kv channels, suggesting that this side chain is a molecular determinant of function. Here, a combination of X-ray crystallography, …
Psychometric Properties Of The 26-Item Eating Attitudes Test (Eat-26): An Application Of Rasch Analysis, Natalie M. Papini, Myungjin Jung, Amanda Cook, Nanette V. Lopez, Lauren T. Ptomey, Stephen D. Herrmann, Minsoo Kang
Psychometric Properties Of The 26-Item Eating Attitudes Test (Eat-26): An Application Of Rasch Analysis, Natalie M. Papini, Myungjin Jung, Amanda Cook, Nanette V. Lopez, Lauren T. Ptomey, Stephen D. Herrmann, Minsoo Kang
Faculty and Student Publications
Background: The 26-item Eating Attitudes Test (EAT-26) is a commonly used tool to assess eating disorder risk. The purpose of this study was to examine the psychometric properties of the EAT-26 with a combined sample: (1) of adults with overweight and obesity enrolled in a behavioral weight loss program and (2) general adult sample (n = 469; age = 36.17 ± 17.83 years; female = 72.5%; white = 66.3%; obese BMI category = 58%). Methods: Rasch modeling was used to assess model-data fit, create an item-person map to evaluate relative distribution items and persons, item difficulty, and person’s eating disorder …
Small-Molecule Protac Mediates Targeted Protein Degradation To Treat Stat3-Dependent Epithelial Cancer, Jinmei Jin, Yaping Wu, Zeng Zhao, Ye Wu
Small-Molecule Protac Mediates Targeted Protein Degradation To Treat Stat3-Dependent Epithelial Cancer, Jinmei Jin, Yaping Wu, Zeng Zhao, Ye Wu
Faculty and Student Publications
The aberrant activation of STAT3 is associated with the etiology and progression in a variety of malignant epithelial-derived tumors, including head and neck squamous cell carcinoma (HNSCC) and colorectal cancer (CRC). Due to the lack of an enzymatic catalytic site or a ligand-binding pocket, there are no small-molecule inhibitors directly targeting STAT3 that have been approved for clinical translation. Emerging proteolysis targeting chimeric (PROTAC) technology-based approach represents a potential strategy to overcome the limitations of conventional inhibitors and inhibit activation of STAT3 and downstream genes. In this study, the heterobifunctional small-molecule-based PROTACs are successfully prepared from toosendanin (TSN), with 1 …
Carum Carvi Modulates Acetaminophen-Induced Hepatotoxicity: Effects On Tnf-Α, Nf-Κb, And Caspases, Tahir Maqbool Mir, Muneeb U. Rehman, Mohammad Khalid Ashfaq, Wajhul Qamar
Carum Carvi Modulates Acetaminophen-Induced Hepatotoxicity: Effects On Tnf-Α, Nf-Κb, And Caspases, Tahir Maqbool Mir, Muneeb U. Rehman, Mohammad Khalid Ashfaq, Wajhul Qamar
Faculty and Student Publications
Carum carvi is a well-known herb traditionally used as a spice in Asian countries. Acetaminophen is a known marketed drug mainly used as an analgesic. It has been scientifically proven that consumption of acetaminophen (paracetamol) is associated with liver toxicity if taken in high doses without medical supervision. The present study evaluated the in vivo antioxidant and hepatoprotective efficacy of Carum carvi against acetaminophen-induced hepatotoxicity in Wistar rats. Our results demonstrate that Carum carvi, at doses (mg/kg) of 100 (D1) and 200 (D2), showed inhibitory properties for DNA-sugar damage, lipid peroxidation, DPPH scavenging, and increased reducing potential in a concentration-dependent …
Development Of A Gc/Q-Tof-Ms Method Coupled With Headspace Solid-Phase Microextraction To Evaluate The In Vitro Metabolism Of Β-Caryophyllene, Joseph Lee, Mei Wang, Goutam Mondal, Ikhlas A. Khan
Development Of A Gc/Q-Tof-Ms Method Coupled With Headspace Solid-Phase Microextraction To Evaluate The In Vitro Metabolism Of Β-Caryophyllene, Joseph Lee, Mei Wang, Goutam Mondal, Ikhlas A. Khan
Faculty and Student Publications
Sample preparation remains both a challenging and time-consuming process in the field of bioanalytical chemistry. Many traditional techniques often require multi-step processes, which can introduce additional errors to the analytical method. Given the complexity of many biological matrices, thorough analyte extraction presents a major challenge to researchers. In the present study, a headspace solid-phase microextraction (HS-SPME) coupled with a GC/Q-ToF-MS method, was developed to quantify in vitro metabolism of β-caryophyllene by both human liver microsome (HLM) and S9 liver fractions. Validation of the method was demonstrated both in terms of linearity (R2 = 0.9948) and sensitivity with a limit of …
Comparative Genomics Of Streptococcus Oralis Identifies Large Scale Homologous Recombination And A Genetic Variant Associated With Infection, Luke R. Joyce, Madison A. Youngblom, Harshini Cormaty, Evelyn Gartstein
Comparative Genomics Of Streptococcus Oralis Identifies Large Scale Homologous Recombination And A Genetic Variant Associated With Infection, Luke R. Joyce, Madison A. Youngblom, Harshini Cormaty, Evelyn Gartstein
Faculty and Student Publications
The viridans group streptococci (VGS) are a large consortium of commensal streptococci that colonize the human body. Many species within this group are opportunistic pathogens causing bacteremia and infective endocarditis (IE), yet little is known about why some strains cause invasive disease. Identification of virulence determinants is complicated by the difficulty of distinguishing between the closely related species of this group. Here, we analyzed genomic data from VGS that were isolated from blood cultures in patients with invasive infections and oral swabs of healthy volunteers and then determined the best-performing methods for species identification. Using whole-genome sequence data, we characterized …
Rosmarinic Acid Prevents Cisplatin-Induced Liver And Kidney Injury By Inhibiting Inflammatory Responses And Enhancing Total Antioxidant Capacity, Thereby Activating The Nrf2 Signaling Pathway, Yifei Xiang, Min Ji, Liqin Wu, Li Lv
Rosmarinic Acid Prevents Cisplatin-Induced Liver And Kidney Injury By Inhibiting Inflammatory Responses And Enhancing Total Antioxidant Capacity, Thereby Activating The Nrf2 Signaling Pathway, Yifei Xiang, Min Ji, Liqin Wu, Li Lv
Faculty and Student Publications
Drug-induced liver and kidney damage is an emergent clinical issue that should be addressed. Rosmarinic acid (RA) has obvious anti-inflammatory and antioxidant effects, so we evaluated the anti-inflammatory and antioxidant effects of RA pretreatment on serum and liver and kidney tissues of cisplatin (CP)-treated mice and explored the possible mechanisms. The results showed that RA pretreatment effectively downregulated the serum, liver, and kidney levels of ALT, AST, BUN, and CRE and the inflammatory factors IL-1β, IL-6, and TNF-α, and simultaneously enhanced the total antioxidant capacity of the liver and kidney. RA pretreatment significantly reduced the levels of MPO, MDA, and …
Evaluation Methods For Vaccination Campaigns On College Campuses: A Scoping Review, Alexcia Carr, Eric Pittman, Hyllore Imeri, Saara Nasruddin, Wesley Sparkmon, Katherine J. Head, Sarah Vivo, Marie Barnard
Evaluation Methods For Vaccination Campaigns On College Campuses: A Scoping Review, Alexcia Carr, Eric Pittman, Hyllore Imeri, Saara Nasruddin, Wesley Sparkmon, Katherine J. Head, Sarah Vivo, Marie Barnard
Faculty and Student Publications
Background: Vaccinations are successful, cost-effective tools to prevent the spread of certain infectious diseases. Many colleges conduct vaccination campaigns on their campuses for various vaccinepreventable diseases, including measles, mumps, influenza, HPV, and most recently, for SARS-CoV-2, the virus responsible for COVID-19. Implementing these campaigns requires substantial effort and understanding their effectiveness is an important factor in justifying these programs.
Aim: This scoping review aims to identify, review, and summarize existing evaluation methods for vaccination campaigns on college campuses in order to provide evaluation guidance for institutions planning future vaccination campaigns.
Methods: Publications that focused on vaccination campaigns on college campuses …
Bioassay-Guided Isolation Of Iridoid Glucosides From Stenaria Nigricans, Their Biting Deterrence Against Aedes Aegypti (Diptera: Culicidae), And Repellency Assessment Against Imported Fire Ants (Hymenoptera: Formicidae), Fazila Zulfiqar, Abbas Ali, Zulfiqar Ali, Ikhlas A. Khan
Bioassay-Guided Isolation Of Iridoid Glucosides From Stenaria Nigricans, Their Biting Deterrence Against Aedes Aegypti (Diptera: Culicidae), And Repellency Assessment Against Imported Fire Ants (Hymenoptera: Formicidae), Fazila Zulfiqar, Abbas Ali, Zulfiqar Ali, Ikhlas A. Khan
Faculty and Student Publications
In our natural product screening program, we screened natural products for their repellency and toxicity against insect vectors. Methanolic extract of aerial parts of Stenaria nigricans (Lam.), with no published chemistry, was tested for repellency against mosquitoes and imported hybrid fire ants. Methanolic extracts showed biting deterrence similar to DEET (N,N-diethyl-3-methylbenzamide) against Aedes aegypti L. Based on this activity, the crude extract was fractionated into chloroform, ethyl acetate, and methanol subfractions. The active methanolic subfraction was further fractionated into 13 subfractions. These fractions were tested for their biting deterrence against Ae. Aegypti. Active subfractions were further characterized to identify the …
Synthesis And Inhibitory Activity Of Machaeridiol-Based Novel Anti-Mrsa And Anti-Vre Compounds And Their Profiling For Cancer-Related Signaling Pathways, Mallika Kumarihamy, Siddharth Tripathi, Premalatha Balachandran, Bharathi Avula
Synthesis And Inhibitory Activity Of Machaeridiol-Based Novel Anti-Mrsa And Anti-Vre Compounds And Their Profiling For Cancer-Related Signaling Pathways, Mallika Kumarihamy, Siddharth Tripathi, Premalatha Balachandran, Bharathi Avula
Faculty and Student Publications
Three unique 5,6-seco-hexahydrodibenzopyrans (seco-HHDBP) machaeridiols A–C, reported previously from Machaerium Pers., have displayed potent activities against methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium, and E. faecalis (VRE). In order to enrich the pipeline of natural product-derived antimicrobial compounds, a series of novel machaeridiol-based analogs (1–17) were prepared by coupling stemofuran, pinosylvin, and resveratrol legends with monoterpene units R-(−)-α-phellandrene, (−)-p-mentha-2,8-diene-1-ol, and geraniol, and their inhibitory activities were profiled against MRSA ATCC 1708, VRE ATCC 700221, and cancer signaling pathways. Compounds 5 and 11 showed strong in vitro activities with MIC values of 2.5 μg/mL and 1.25 μg/mL against MRSA, respectively, and …
Hiv-1 Tat Upregulates The Receptor For Advanced Glycation End Products And Superoxide Dismutase-2 In The Heart Of Transgenic Mice, Alaa N. Qrareya, Nason S. Wise, Emmanuel R. Hodges, Fakhri Mahdi
Hiv-1 Tat Upregulates The Receptor For Advanced Glycation End Products And Superoxide Dismutase-2 In The Heart Of Transgenic Mice, Alaa N. Qrareya, Nason S. Wise, Emmanuel R. Hodges, Fakhri Mahdi
Faculty and Student Publications
Cardiovascular disorder (CVD) is a common comorbidity in people living with HIV (PLWH). Although the underlying mechanisms are unknown, virotoxic HIV proteins, such as the trans-activator of transcription (Tat), likely contribute to CVD pathogenesis. Tat expression in mouse myocardium has been found to induce cardiac dysfunction and increase markers of endothelial toxicity. However, the role that Tat may play in the development of CVD pathogenesis is unclear. The capacity for Tat to impact cardiac function was assessed using AC16 human cardiomyocyte cells and adult male and female transgenic mice that conditionally expressed Tat [Tat(+)], or did not [Tat(−)]. In AC16 …
Immunomodulatory Effects Of Plant Extracts From Salvia Deserta Schang. And Salvia Sclarea L., Aizhan Zhussupova, Gaziza Zhumaliyeva, Vyacheslav Ogay, Assel Issabekova
Immunomodulatory Effects Of Plant Extracts From Salvia Deserta Schang. And Salvia Sclarea L., Aizhan Zhussupova, Gaziza Zhumaliyeva, Vyacheslav Ogay, Assel Issabekova
Faculty and Student Publications
Medicines, their safety, effectiveness and quality are indispensable factors of national security, important on a global scale. The COVID-19 pandemic has once again emphasized the importance of improving the immune response of the body in the face of severe viral infections. Plants from the Salvia L. genus have long been used in traditional medicine for treatment of inflammatory processes, parasitic diseases, bacterial and viral infections. The aim of the current study was to evaluate the immunomodulatory effects of plant extracts LS-1, LS-2 from Salvia deserta Schang. and LS-3, LS-4 from Salvia sclarea L. plants growing in southern Kazakhstan by conventional …
Phytochemical Investigation Of Egyptian Spinach Leaves, A Potential Source For Antileukemic Metabolites: In Vitro And In Silico Study, Shimaa M. Abdelgawad, Mona H. Hetta, Mohamed A. Ibrahim, Premalatha Balachandran
Phytochemical Investigation Of Egyptian Spinach Leaves, A Potential Source For Antileukemic Metabolites: In Vitro And In Silico Study, Shimaa M. Abdelgawad, Mona H. Hetta, Mohamed A. Ibrahim, Premalatha Balachandran
Faculty and Student Publications
Spinacia oleracea L., Amaranthaceae, leaves cultivated in Egypt demonstrated a potential antileukemic activity against the chronic myeloid leukemia, K562 cell line. Thus, the aim of this study is to carry out a phytochemical investigation of S. oleracea leaves as well as the isolation of its antileukemic phytoconstituents. Phytochemical investigation of S. oleracea leaves resulted in the isolation of seventeen known compounds. The biological study revealed that compounds hexaprenol, phytol, and 18-[(1-oxohexadecyl) oxy]-9-octadecenoic acid exhibited a remarkable antiproliferative activity against K562 cells in vitro. A mechanistic in silico study showed that hexaprenol, phytol, and 18-[(1-oxohexadecyl) oxy]-9-octadecenoic acid exhibited a strong binding …