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The Thai Journal of Veterinary Medicine

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Articles 151 - 180 of 2524

Full-Text Articles in Medicine and Health Sciences

Quantitative Assessment Of Preoperative And Postoperative Brachycephalic Airway Obstruction Syndrome Surgery Using Clinical Grading Questionnaires And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Pasakorn Brikshavana, Sumit Durongphongtorn Aug 2024

Quantitative Assessment Of Preoperative And Postoperative Brachycephalic Airway Obstruction Syndrome Surgery Using Clinical Grading Questionnaires And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Pasakorn Brikshavana, Sumit Durongphongtorn

The Thai Journal of Veterinary Medicine

Brachycephalic airway obstructive syndrome (BAOS) is an anatomical abnormality of the upper airway commonly found in brachycephalic breeds, leading to clinical signs and physical capacity impairment. The degree of stenotic nares was reported to be related to BAOS severity in French bulldogs. This study aimed to compare clinical signs and physical capacity in French bulldogs before and after BAOS surgery using modified Roedler questionnaires and a 6-minute walk test (6-MWT). Twenty-seven French bulldogs were enrolled in this study (non-BAOS control (n=7), non-severe stenotic nares BAOS dogs (n=8), and severe stenotic nares BAOS dogs (n=12). BAOS surgery included staphylectomy using bipolar …


Conventional Vs Three-Dimensional Computed Tomography For Measurement Of Tibial Tuberosity To Trochlear Groove Distance In Small Breed Dogs With And Without Medial Patellar Luxation, Radka S. Garnoeva Aug 2024

Conventional Vs Three-Dimensional Computed Tomography For Measurement Of Tibial Tuberosity To Trochlear Groove Distance In Small Breed Dogs With And Without Medial Patellar Luxation, Radka S. Garnoeva

The Thai Journal of Veterinary Medicine

The operative technique for medial patellar luxation (MPL) is dependent on the degree of accompanying bone deformities. The most frequent abnormalities: the shallow trochlear groove and tibial tuberosity medialisation are corrected surgically with trochleoplasty and tibial tuberosity transposition, respectively. The aim of the present study was to determine the distance between the tibial tuberosity and the trochlear groove (TT-TG) on two-dimensional axial computed tomography (CT) scans and three-dimensional multiplanar reconstruction images from 21 stifles from healthy dogs and 15 stifles from dogs with grade 2 MPL and to compare the performance of both methods. Two axial CT scans of the …


Seroprevalence Of Lawsonia Intracellularis Antibodies Among Large-Scale Swine Farming Systems In Thailand, Thanh Che Nguyen, Chairani Ridha Maghfira, Nathaya Young, Kanokwan Samatiwat, Chaiyan Kaewpaluk, Apisak Laothanakit, Pawarisa Mungprasittichai, Roongroje Thanawongnuwech, Yaowalak Panyasing Aug 2024

Seroprevalence Of Lawsonia Intracellularis Antibodies Among Large-Scale Swine Farming Systems In Thailand, Thanh Che Nguyen, Chairani Ridha Maghfira, Nathaya Young, Kanokwan Samatiwat, Chaiyan Kaewpaluk, Apisak Laothanakit, Pawarisa Mungprasittichai, Roongroje Thanawongnuwech, Yaowalak Panyasing

The Thai Journal of Veterinary Medicine

Gastrointestinal infectious diseases caused by bacteria have been a major problem in the swine industry, including Thailand. Porcine proliferative enteropathy (PPE), also known as porcine ileitis, is an enteric infectious disease caused by Lawsonia intracellularis – an obligate intracellular bacterium. PPE has been an economic threat to the global pork production system due to various types of diarrheas. Serological diagnosis can be applied to the seroprevalence of pathogen-antibodies and the timing of L. intracellularis infection when introduced to the herd. This present study showed the current serological status and the seroprevalence of antibodies against L. intracellularis in large-scale Thai swine …


Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors, Gizem Kırmızıoğlu, Iraz Akış Aug 2024

Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors, Gizem Kırmızıoğlu, Iraz Akış

The Thai Journal of Veterinary Medicine

Mammary tumors are the most common neoplasm in dogs, with a high mortality rate. The development of canine mammary tumors (CMT) is multifactorial, but different incidence rates between breeds suggest the effects of genetic risk factors. Many CMT-associated candidate genes were reported, mainly involved in cell cycle control. This study aims to investigate p27 tumor suppressor gene polymorphisms in dogs with mammary tumors and analyze the association between variations and CMTs. For this purpose, case and control groups were formed from 22 dogs diagnosed with malignant mammary tumors and 10 dogs with healthy mammary glands. The whole canine p27 gene …


Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti Aug 2024

Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti

The Thai Journal of Veterinary Medicine

Flavonol quercetin (Q), found in vegetables and fruits, demonstrates the most potent anti-inflammatory effect. The preventive effect and cellular mechanisms of Q (Q1, Q10, and Q100 µM) on IL-1β induced leaky gut via the signaling molecules of inflammatory pathway, NF-κB p65-iκB/or p38/ERK1/2/MLCK/phospho(p)-MLC were investigated in Caco-2 cells. The cytotoxic effect of Q was initially determined by MTT assay. Transepithelial electrical resistance (TER) and apical-to-basolateral fluorescein isothiocyanate-labeled dextran (FD-4) flux were used to determine paracellular permeability. The protein expression of signaling molecules was examined by Western blot analysis, and their target MLCK mRNA expression was determined using real-time PCR. All Q …


Impacts Of Species, Gender Disparities, And Covid-19 Lockdowns On Population Control: Insights From 337,590 Dogs And Cats In Bangkok Metropolitan Administration (Bma), Thailand (2009-2023), Shanaporn Leelakajornkit, Natchanon Dumniem, Permsak Wataganara, Siwa Maison, Suppawiwat Ponglowhapan Aug 2024

Impacts Of Species, Gender Disparities, And Covid-19 Lockdowns On Population Control: Insights From 337,590 Dogs And Cats In Bangkok Metropolitan Administration (Bma), Thailand (2009-2023), Shanaporn Leelakajornkit, Natchanon Dumniem, Permsak Wataganara, Siwa Maison, Suppawiwat Ponglowhapan

The Thai Journal of Veterinary Medicine

Surgical sterilization stands as a key solution to address the overpopulation of dogs and cats, with numerous influencing factors impacting the success of population control efforts, including species, gender, and financial conditions. The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), or COVID-19, in 2020 has significantly altered human life and impacted pet animals. Lockdown restrictions were implemented in various countries, including Thailand, affecting the cumulative data of neutered animals, which was subsequently analyzed in this study. There were 337,590 dogs and cats from 2009 to 2023, and the data were systematically categorized by years, species, and gender. …


Clinical Response To Oclacitinib In Canine Cutaneous Epitheliotropic T-Cell Lymphoma: A Case Reports, Takuma Imai Aug 2024

Clinical Response To Oclacitinib In Canine Cutaneous Epitheliotropic T-Cell Lymphoma: A Case Reports, Takuma Imai

The Thai Journal of Veterinary Medicine

The chemotherapy for canine cutaneous epitheliotropic T cell lymphoma (CETL) might cause unpleasant side effects or fail to produce positive results. We attempted a different approach with a more tolerable treatment option. We administered a high dose of oclacitinib (1.6 mg/kg/twice daily) to a patient diagnosed with CETL through histopathological examination and immunocytochemical staining. The patient had previously shown a poor response to chemotherapy and severe side effects to steroids. Within one month of starting oclacitinib treatment, we observed significant improvement in clinical symptoms, followed by improved appetite, increased activity, and weight gain after 2.5 months. However, subsequently, the patient's …


A Case Of Conjunctivitis Due To Mdr-Mr Staphylococcus Pseudintermedius In A Dog, Hazel Tamakan Yesi̇lovali, Hali̇t Sukur Aug 2024

A Case Of Conjunctivitis Due To Mdr-Mr Staphylococcus Pseudintermedius In A Dog, Hazel Tamakan Yesi̇lovali, Hali̇t Sukur

The Thai Journal of Veterinary Medicine

A young male Dachshund dog who came to the animal hospital was diagnosed with conjunctivitis on clinical examination. Methicillin-resistant Staphylococcus pseudintermedius was identified as a result of culture and bacteriological examination of the conjunctival swab, and then antimicrobial susceptibility test. Methicillin resistant S. pseudintermedius identified with the VITEK-II Compact device was also confirmed by molecular methods so that susceptible antibiotics were selected for the treatment of the patient and reported. In accordance with the antimicrobial susceptibility results, the patient was treated with an eye drop preparation containing neomycin. This is the first report of a canine conjunctivitis caused by multidrug-methicillin …


Computed Tomography And Magnetic Resonance Imaging Of Maxillofacial Rhabdomyosarcoma And Suspected Multiple Metastases Including The Epidural Space And Bone Marrow In A Dog, Sang-Kwon Lee, Kija Lee Aug 2024

Computed Tomography And Magnetic Resonance Imaging Of Maxillofacial Rhabdomyosarcoma And Suspected Multiple Metastases Including The Epidural Space And Bone Marrow In A Dog, Sang-Kwon Lee, Kija Lee

The Thai Journal of Veterinary Medicine

A 3-year-old mixed-breed dog presenting with a recurrent maxillofacial tumor was diagnosed with recurrent rhabdomyosarcoma. Computed tomography and magnetic resonance imaging revealed a left maxillofacial mass, left cervical lymph node enlargement, epidural mass dorsal to the 6th lumbar vertebra compressing the cauda equina, soft-tissue mass ventral to the 6th lumbar vertebra, multiple subcutaneous nodules and signal changes in the paravertebral muscles. The left maxilla, lumbar spine and ilium exhibited diffuse signal changes and contrast enhancement upon magnetic resonance imaging. This is, to our knowledge, the first reported case of bone marrow and epidural-space involvement of a canine rhabdomyosarcoma.


Detection Of Mycoplasma Synoviae Antibodies In Day-Old Chicks Using Rapid Serum Agglutination Assay And Enzyme-Linked Immunosorbent Assay, Kriengwich Limpavithayakul, Somsak Pakpinyo Aug 2024

Detection Of Mycoplasma Synoviae Antibodies In Day-Old Chicks Using Rapid Serum Agglutination Assay And Enzyme-Linked Immunosorbent Assay, Kriengwich Limpavithayakul, Somsak Pakpinyo

The Thai Journal of Veterinary Medicine

Mycoplasma synoviae antibodies detection using rapid serum agglutination (RSA) assay and enzyme-linked immunosorbent assay (ELISA) is considered as the suitable screening tool in chicken flocks. Both RSA assay and ELISA have performed an ability to detect an antibody formation during mycoplasma infection due to their sensitivity in detecting both IgM and IgY. However, the IgY is the major antibody isotype transferred from the hen to the newly hatched broiler chick via egg yolk. The amount of maternal IgY circulating in the chicks’ serum is approximately 30% of the IgY level present in the hen’s bloodstream. This study was conducted to …


High-Speed Dental Drill May Be An Acceptable Instrument Performing Canine Laminectomy, Ziyao Zhou Aug 2024

High-Speed Dental Drill May Be An Acceptable Instrument Performing Canine Laminectomy, Ziyao Zhou

The Thai Journal of Veterinary Medicine

Laminectomy is usually used in spinal cord dysfunction like intervertebral disc herniation. High-speed pneumatic surgical drill is the most common surgical instrument for canine laminectomy operation. However, since the finical problem, the professional instrument has a low installation rate in animal hospitals in developing country. In this study, we hypothesized that dental drill may replace pneumatic surgical drills in dorsal laminectomy and hemilaminectomy, observed if dorsal laminectomy and hemilaminectomy can been successfully operated by dental drill, and compared the canine dorsal laminectomy and hemilaminectomy. Operations were performed in L2-L3 of lumbar vertebra among 12 Chinese rural dogs (6 for dorsal …


Reducing The Effects Of Oxidative And Nitrosative Stress On Viability Of The Broiler Blood Cells When Exposed To High Ambient Temperatures By Using Tannic Acid, Jaroon Wandee, Piyarat Srinontong, Worapol Aengwanich Aug 2024

Reducing The Effects Of Oxidative And Nitrosative Stress On Viability Of The Broiler Blood Cells When Exposed To High Ambient Temperatures By Using Tannic Acid, Jaroon Wandee, Piyarat Srinontong, Worapol Aengwanich

The Thai Journal of Veterinary Medicine

The study aimed to investigate the effect of tannic acid (TA) on total antioxidant power (FRAP), malondialdehyde (MDA), hydrogen peroxide (H2O2), nitric oxide (NO), and the viability of broiler blood cells (BBC) exposed to high ambient temperatures. The BBC from a broiler chicken was exposed to temperatures ranging from 41.5°C to 46°C and incubated with TA at concentrations of 0 (the positive control group, TPCG), 5, 10, 15, and 20 μg/mL. Results showed that MDA, H2O2, and NO levels of the control group (TCG) which BBC was maintained at 41.5°C were significantly lower than TPCG. The viability of TPCG was …


Biomechanical Comparison Of Transosseous Four-Strand Tendon Suture Patterns For Repair Of Ruptured Distal Common Calcaneal Tendon: An Ex Vivo Study In Dogs, Tae-Hwan Ahn, Min-Yeong Lee, Jaekyoung Lee, Hwi-Yool Kim Aug 2024

Biomechanical Comparison Of Transosseous Four-Strand Tendon Suture Patterns For Repair Of Ruptured Distal Common Calcaneal Tendon: An Ex Vivo Study In Dogs, Tae-Hwan Ahn, Min-Yeong Lee, Jaekyoung Lee, Hwi-Yool Kim

The Thai Journal of Veterinary Medicine

There is no golden standard method of suturing for distal common calcaneal tendon rupture in dogs. Thus this study aimed to evaluate and compare the mechanical characteristics of three distinct transosseous suture (TOS) repair techniques, which were specifically developed to treat ruptured distal common calcaneal tendons and to identify golden standard suturing method. The study was performed using 30 hind limbs obtained from 15 canine cadavers with weights ranging between 7.9 kg and 11.4 kg. The repair methods using modified Becker suture (group 1), modified Krackow suture (group 2), and modified double-Tsuge suture (group 3) were randomly assigned. Each group …


Efficacy Of In-House Nanosilver Polyurethane Hydrogel Dressing On Second-Degree Burn Wounds In A Porcine Model, Pasakorn Brikshavana, Tapanat Songkasupa, Pleansaung Vichit, Onrampa Supsombon, Arunrode Nilrattanaprasert, Theerayuth Kaewamatawong Aug 2024

Efficacy Of In-House Nanosilver Polyurethane Hydrogel Dressing On Second-Degree Burn Wounds In A Porcine Model, Pasakorn Brikshavana, Tapanat Songkasupa, Pleansaung Vichit, Onrampa Supsombon, Arunrode Nilrattanaprasert, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the effects of hydrogel containing nanosilver particles on a second-degree burn wound in a porcine model. The second degree burn wound was performed using 1 cm in diameter, on 100 °C heated cylindrical brass bar applied to the dorsum of pig skin. Burn wounds were randomly divided into three groups of wound dressings (WDs) including commercial polyurethane hydrogel (Cutinova Hydro®, Gr. A), in-house hydrogel (Gr. B) and in-house nanosilver hydrogel (Gr. C). The wound contraction rate and hyperemic zone were measured and investigated at 3, 7, 14 and 21 days after dressing. …


In-House Polydimethylsiloxane Microfluidic Device On Single Cell Trapping And Culturing Of Leukemia Cell Line: Comparison Between Conventional And Conventional With Centrifugal Methods, Alongkorn Pimpin, Sudchaya Bhanpattanakul, Tepparit Wongpakham, Chomkwan Tawatcharaporn, Thanawit Siha-Umphai, Mookdapa Sinsiriphan, Atikan Chalermchuang, Tewan Tongmanee, Attawut Thanomsridetchai, Thammawit Suwannaphan, Witsaroot Sripumkhaie, Pattaraluck Pattamang, Mayuree Chanasakulniyom, Praprudee Piyaviriyakul, Wutthinun Jeamsaksiri, Achariya Sailasuta, Theerawat Tharasanit, Theerayuth Kaewamatawong Aug 2024

In-House Polydimethylsiloxane Microfluidic Device On Single Cell Trapping And Culturing Of Leukemia Cell Line: Comparison Between Conventional And Conventional With Centrifugal Methods, Alongkorn Pimpin, Sudchaya Bhanpattanakul, Tepparit Wongpakham, Chomkwan Tawatcharaporn, Thanawit Siha-Umphai, Mookdapa Sinsiriphan, Atikan Chalermchuang, Tewan Tongmanee, Attawut Thanomsridetchai, Thammawit Suwannaphan, Witsaroot Sripumkhaie, Pattaraluck Pattamang, Mayuree Chanasakulniyom, Praprudee Piyaviriyakul, Wutthinun Jeamsaksiri, Achariya Sailasuta, Theerawat Tharasanit, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

To develop a suitable model to study biological and characterization of Cancer stem cells (CSCs), single cell trapping and culturing using microfluidic techniques have become a high-through put system that can manage and control microenvironment and practical intracellular analysis. The aim of this study was to develop and validate a microfluidic device for single cell trapping and culturing using the leukemia cell line as a cell model. Comparison between conventional and conventional with centrifugal methods was also analyzed. To compare trapping efficacy between these 2 methods, leukemia cell concentration of 1.5×106 cells/ml was loaded with a flow rate of 0.25 …


Molecular Characterization And Antibiogram Of Riemerella Anatipestifer In Ducklings In Upper Egypt, Mohamed Sabry Ahmed, Amal Abd El-Ghani Batahi, Ahmed Ibrahim Ahmed, Nabila Osman Aug 2024

Molecular Characterization And Antibiogram Of Riemerella Anatipestifer In Ducklings In Upper Egypt, Mohamed Sabry Ahmed, Amal Abd El-Ghani Batahi, Ahmed Ibrahim Ahmed, Nabila Osman

The Thai Journal of Veterinary Medicine

Riemerella anatipestifer infection represents a great threat to the duck industry worldwide and it is considered the major cause of death in the backyard rearing system of ducks in Egypt. The disease is characterized by diarrhea, respiratory signs and loss of weight. The present study was conducted during the period between July 2019 and January 2021 to examine the prevalence rate, the antibiogram patterns of the different antibiotics and the pathogenicity of R. anatipestifer in Qena province. One-hundred samples were collected from diseased and freshly dead backyard ducklings. A tentative characterization of R. anatipestifer was done depending on the phenotypic …


The Benefits Of Dexmedetomidine For Pain Mitigation After Cautery Disbudding In Calves, Tsung-Ching Liu, Chen-Fei Chiang, Thomas Chia-Tang Ho, Jacky Peng-Wen Chan Aug 2024

The Benefits Of Dexmedetomidine For Pain Mitigation After Cautery Disbudding In Calves, Tsung-Ching Liu, Chen-Fei Chiang, Thomas Chia-Tang Ho, Jacky Peng-Wen Chan

The Thai Journal of Veterinary Medicine

The purpose of this study was to determine the effect of dexmedetomidine (DEX) alone on pain mitigation following cautery disbudding in calves. A total of 21 calves were randomly divided into three groups: sham-disbudded control with placebo (S, n = 8); hot-iron disbudded after placebo (D, n = 6); hot-iron disbudded after 0.01 mg/kg of DEX (DeD, n = 7). The pain-related responses measured included serum cortisol levels, struggling scores, pain-related behaviors and other physiological values. The results indicated that the struggling scores, the cortisol concentrations from +10 to +60 min after disbudding and the area under curve of cortisol …


Interlocking Nail Stabilization Technique For Long Bone Fractures In Calves, Mustafa Arican, Semih Altan, Kurtuluş Parlak, Fahrettin Alkan Aug 2024

Interlocking Nail Stabilization Technique For Long Bone Fractures In Calves, Mustafa Arican, Semih Altan, Kurtuluş Parlak, Fahrettin Alkan

The Thai Journal of Veterinary Medicine

The aim of this study was to report the clinical and radiographic outcome from using an interlocking nail (ILN) technique for the stabilization of long bone fractures in 15 calves. Forty-eight calves with fractures were presented to the large animal hospital. Fifteen calves of different breeds, age, sex and weight with front or hind limb fractures were considered suitable for ILN application. These included proximal or distal diaphyseal transverse and oblique fractures of the humerus, radius, femur and tibia. The third generation of six, eight, nine and 10mm diameter interlocking nails were used in this study. Interlocking pins were used …


The Effect Of Intranasal Medetomidine/Ketamine And Diazepam/Ketamine Combinations On Tear Production And Intraocular Pressure In Dogs, Osman Bulut, Rahime Yaygingul Aug 2024

The Effect Of Intranasal Medetomidine/Ketamine And Diazepam/Ketamine Combinations On Tear Production And Intraocular Pressure In Dogs, Osman Bulut, Rahime Yaygingul

The Thai Journal of Veterinary Medicine

To determine the effect of intranasal medetomidine/ketamine and diazepam/ketamine combinations on tear production and intraocular pressure in dogs. The animal material included 28 dogs of different breeds, ages, weights and genders that were brought to the surgery department of the Adnan Menderes University, Faculty of Veterinary Medicine for simple anesthesia indications but were otherwise healthy ocularly. In a crossover design, the dogs were randomly assigned to 4 groups of 7 dogs. Animals in the first group were given 50 μg/kg of medetomidine and 10 mg/kg of ketamine intramuscularly (MEK-IM). The second group of animals was given 50 μg/kg of medetomidine …


Productivity Of Young Broilers Fed Phyllanthus Niruri Linn And Melaleuca Cajuput Leaf Meal As A Phytogenic Feed Additive, Listya Purnamasari Aug 2024

Productivity Of Young Broilers Fed Phyllanthus Niruri Linn And Melaleuca Cajuput Leaf Meal As A Phytogenic Feed Additive, Listya Purnamasari

The Thai Journal of Veterinary Medicine

Feed additives from medicinal plants can be used as alternative antibiotics to optimize productivity, growth, and health of livestock. Medicinal plants Phyllanthus niruri Linn (Meniran) and Melaleuca cajuput (Kajuput) as alternative growth promoters contain antioxidants and antimicrobials to improve the quality of animal products. The aim of the study was to evaluate the effect of medicinal plant leaf meals as phytogenic feed additives on broilers’ productivity and internal organ parameters. For 21 days fed, 80 1-day-old Loughmann broilers (MB202) unsex were distributed in 4 treatments with 4 pens per treatment and 5 birds per pen. The dietary treatments were: T1 …


A Study On Stress-Induced By Anaesthesia And Surgery Training Using Live Animals In A Veterinary School, Krittee Dejyong, Jutapoln Sunghan, Nantarat Chutijiratthitikan, Chonnakarn Jatchavala Aug 2024

A Study On Stress-Induced By Anaesthesia And Surgery Training Using Live Animals In A Veterinary School, Krittee Dejyong, Jutapoln Sunghan, Nantarat Chutijiratthitikan, Chonnakarn Jatchavala

The Thai Journal of Veterinary Medicine

The veterinary curriculum includes an anesthesia and surgery course that utilizes live animal laboratories and euthanasia, potentially causing stress in students. We used a validated psychology questionnaire to assess their stress levels, confirming that live animal laboratories induce significant stress. Forty-two fourth year veterinary students, including individuals who identified as male, female, and LGBTQ, and who held religious beliefs in Buddhism and Islam, volunteered to participate in the study. The study had received ethical approval from the Human Research Ethics Committee at Prince of Songkla University. After series of stress questionnaires before and after Veterinary surgery and anaesthesia class, female …


An Anatomical, Histological, And Immunohistochemical Investigation On The Intestines Of The Eurasian Lynx (Lynx Lynx), Şükran Yedi̇el Aras, Gülseren Kırbaş Doğan, Ebru Karadağ Sarı Aug 2024

An Anatomical, Histological, And Immunohistochemical Investigation On The Intestines Of The Eurasian Lynx (Lynx Lynx), Şükran Yedi̇el Aras, Gülseren Kırbaş Doğan, Ebru Karadağ Sarı

The Thai Journal of Veterinary Medicine

In this study, it was aimed to perform an anatomical, histological, and immunohistochemical investigation of the intestines of the lynx. After performing macro-anatomical measurements of the tissues obtained from the small and large intestines of the lynx which was brought to the Kafkas University Veterinary Faculty Clinics and Wild Life Preservation and Rehabilitation Center for treatment but could not be saved despite all interventions, the routine histological procedure was followed. Triple staining was applied to examine the general histological structure of intestinal tissues, and PAS staining was applied for identifying goblet cells. Somatostatin distribution was examined immunohistochemically. The lengths and …


Characteristics Of Extended-Spectrum Beta-Lactamase (Esbl)- Producing Escherichia Coli Associated With Antimicrobial Use In Captive Wild Animals, Montira Yossapol, Jinnapat Imidom, Kannarun Sakhorn, Pimpatcha Thanomwat, Sarawut Sonrit, Siraphop Labmala, Tarid Purisotayo, Natapol Pumipuntu, Justice Opare Odoi, Adithep Konputtar Aug 2024

Characteristics Of Extended-Spectrum Beta-Lactamase (Esbl)- Producing Escherichia Coli Associated With Antimicrobial Use In Captive Wild Animals, Montira Yossapol, Jinnapat Imidom, Kannarun Sakhorn, Pimpatcha Thanomwat, Sarawut Sonrit, Siraphop Labmala, Tarid Purisotayo, Natapol Pumipuntu, Justice Opare Odoi, Adithep Konputtar

The Thai Journal of Veterinary Medicine

Antimicrobial agents are a cause of the increase in antimicrobial-resistant (AMR) bacteria. We investigated the prevalence and characteristics of extended-spectrum beta-lactamase (ESBL)-producing bacteria in captive wild animals before and after exposure to antimicrobial agents. Sixty-six fecal samples were collected from captive animals (spotted deer, Rusa deer, geese, chickens, rabbits, and guinea pigs) at the Mahasarakham University Mini Zoo in Thailand on three sampling occasions: October 2021, January 2022, and April 2022. Using MacConkey agar supplemented with cefotaxime, ESBL-producing Escherichia coli (ESBL-Ec) was isolated and subjected to antimicrobial susceptibility tests and genetic analysis. The results showed a high prevalence of ESBL-Ec …


Protective Immunity Of The Pore-Forming Domains Of Actinobacillus Pleuropneumoniae Apx Toxins In A Mouse Model, Kamonpun Chuekwon, Li-Ting Cheng, Chun-Yen Chu Aug 2024

Protective Immunity Of The Pore-Forming Domains Of Actinobacillus Pleuropneumoniae Apx Toxins In A Mouse Model, Kamonpun Chuekwon, Li-Ting Cheng, Chun-Yen Chu

The Thai Journal of Veterinary Medicine

The hemolytic/leukolytic toxins ApxI, II, and III play a central role in the pathogenicity of Actinobacillus pleuropneumoniae and may serve as effective vaccine antigens. However, since these toxins are large in size, we aimed to determine whether only the pore-forming domain of the toxins may be sufficient for protective immunity. Mice were vaccinated with the pore-forming domains for immune response analysis and challenge test. Significant antibody response was observed for the ApxI and II vaccine groups. For cellular immune response, CD4+ and CD8+ T cell expansion was observed for ApxI. Pro-inflammatory cytokine (IL-1b, IL-6) and TH2-type …


Discovery Of Chicken Proventricular Necrosis Virus From Transmissible Viral Proventriculitis Outbreak In Broilers, Thailand, ์Nantaporn Wandee, Preeda Lertwatchrasarakul, Thaweesak Songserm Aug 2024

Discovery Of Chicken Proventricular Necrosis Virus From Transmissible Viral Proventriculitis Outbreak In Broilers, Thailand, ์Nantaporn Wandee, Preeda Lertwatchrasarakul, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

Transmissible viral proventriculitis (TVP) is an infectious disease-causing retarded growth in chickens. In Thailand, many stunted chickens have been found on commercial broiler farms since 2017. Significant post-mortem lesions are found only in the proventriculus. This study aims to investigate the causative agents of TVP outbreaks focusing on chicken proventricular necrosis virus (CPNV), the possible significant cause of TVP. CPNV was reproduced in specific pathogen-free (SPF) chickens. Affected chickens that have undergone proventriculitis are CPNV-positive by RT-PCR and nucleotide sequencing followed by phylogenetic analysis. The result showed that proventriculitis could be experimentally reproduced by inoculation of proventricular homogenate obtained from …


Effect Of Rosmarinic Acid On Dog Semen Quality After Freeze-Drying By Applying Freeze-Drying Machines For Vaccine Production, Janjira Phavaphutanon, Phannipa Phungsombun, Kornchai Kornkaewrat, Sudtisa Laopiem, Wilairat Chumsing, Pattra Moonjit Aug 2024

Effect Of Rosmarinic Acid On Dog Semen Quality After Freeze-Drying By Applying Freeze-Drying Machines For Vaccine Production, Janjira Phavaphutanon, Phannipa Phungsombun, Kornchai Kornkaewrat, Sudtisa Laopiem, Wilairat Chumsing, Pattra Moonjit

The Thai Journal of Veterinary Medicine

Freeze-drying (FD) is a sperm preservation method that eliminates the need for liquid nitrogen. However, the FD process can expose sperm to free radicals and temperature variations. This study aimed to determine the impact of rosmarinic acid (RA) as an antioxidant, initial sperm motility, and storage temperature on the DNA integrity of freeze-dried dog sperm. Forty-four ejaculates from 15 dogs of various breeds were divided into control (FD extender) and RA (FD extender with RA) groups, further categorized by motility (above or below 70%). After FD, samples were stored for three months at room temperature and 4 °C. Sperm quality …


Relationship Of Hyperlipidemia And Gallbladder Sludge Scores In Dogs With Hyperadrenocorticism, Jayeop Jo, Dohee Lee, Taesik Yun, Yoonhoi Koo, Yeon Chae, Dongwoo Chang, Byeong-Teck Kang, Mhan-Pyo Yang, Kyung-Duk Min, Hakhyun Kim Aug 2024

Relationship Of Hyperlipidemia And Gallbladder Sludge Scores In Dogs With Hyperadrenocorticism, Jayeop Jo, Dohee Lee, Taesik Yun, Yoonhoi Koo, Yeon Chae, Dongwoo Chang, Byeong-Teck Kang, Mhan-Pyo Yang, Kyung-Duk Min, Hakhyun Kim

The Thai Journal of Veterinary Medicine

Hyperadrenocorticism (HAC) and hyperlipidemia have been identified as the causes of cholestatic disease in dogs. However, the possibility of direct association of HAC with a cholestatic disease or secondary hyperlipidemia-induced cholestatic disease in dogs with HAC has not been elucidated. This study aimed to evaluate the differences in serum triglyceride (TG), total cholesterol (TC) and basal cortisol (BC) concentrations based on the gallbladder (GB) sludge score in dogs with HAC. This study was a retrospective cohort study. In total, 36 dogs with pituitary-dependent hyperadrenocorticism (PDH) were divided into six groups (GB scores 0–5) based on the ultrasonographic evaluation of the …


Effects Of Prostaglandin Administration After Farrowing On Milk Output, Vaginal Discharge, And Mastitis During The Early Postpartum Period In Sows Under Field Conditions, G. Romano, R. Saleri, F. De Rensis, E. Dall’Olio, Padet Tummaruk, M. Andrani, C. Mazzoni Aug 2024

Effects Of Prostaglandin Administration After Farrowing On Milk Output, Vaginal Discharge, And Mastitis During The Early Postpartum Period In Sows Under Field Conditions, G. Romano, R. Saleri, F. De Rensis, E. Dall’Olio, Padet Tummaruk, M. Andrani, C. Mazzoni

The Thai Journal of Veterinary Medicine

The present study evaluates the clinical effects of early post-partum prostaglandin administration on milk output, vaginal discharge and mastitis in sow. Two groups of sows were used: sows (n = 25) treated with prostaglandin F-2alpha (PGF-2α) intramuscularly 36 h after farrowing and a control group (n = 48) that did not receive any treatment. Seventy-two hours after farrowing, all animals were scored for rectal temperature, respiratory rate, feed ingestion, milk output, vaginal discharge and mastitis. Rectal temperature, feed intake, and milk output did not differ between the groups (P = 0.43). Regarding the clinical data, vaginal discharge was detected in …


Symmetric Dimethylarginine In Dogs With Pulmonary Arterial Hypertension, Siwayu Rattanakanokchai, Numfa Fungbun, Ketmanee Senaphan, Supranee Jitpean, Trasida Ployngam Jun 2024

Symmetric Dimethylarginine In Dogs With Pulmonary Arterial Hypertension, Siwayu Rattanakanokchai, Numfa Fungbun, Ketmanee Senaphan, Supranee Jitpean, Trasida Ployngam

The Thai Journal of Veterinary Medicine

Pulmonary arterial hypertension (PAH) causes impaired kidney function in humans. However, this occurrence has not been well investigated in PAH dogs. Serum symmetrical dimethylarginine (SDMA) is one of the early biomarkers of kidney impairment. The present study aims to evaluate SDMA levels of first diagnosed PAH (n=14) compared with healthy dogs (n=12) and to analyze the correlation between SDMA levels and significant echocardiographic parameters of PAH dogs. The mean SDMA of PAH dogs was statistically higher than that of healthy dogs (P = 0.003), while the mean serum creatine level had no difference among groups (P = 0.456). Precapillary PAH …


Normal Thymus Evaluation Using Radiographs And Computed Tomographic Images In Young And Adult Dogs, Yoenhee Park, Sang-Kwon Lee, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee Jun 2024

Normal Thymus Evaluation Using Radiographs And Computed Tomographic Images In Young And Adult Dogs, Yoenhee Park, Sang-Kwon Lee, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee

The Thai Journal of Veterinary Medicine

The thymus generally presents as a sail-shaped soft-tissue opacity on thoracic radiographs in young, pre-pubescent dogs. After puberty, lymphatic tissue is replaced by adipose tissue and can usually no longer be identified on radiographs; however, thymic remnants have often been observed in adult dogs. The objective of this retrospective descriptive study was to investigate the incidence of thymic remnants using thoracic radiographs and to analyze the incidence in dogs by age, sex and breed. We analyzed the data from 1,464 dogs that underwent both thoracic ventrodorsal and right lateral radiography, of which 40 underwent both radiography and computed tomography (CT). …