Medicine and Health Sciences Commons^™

Adolescent Decision Making And Risk Behavior: A Neurobiological Approach, Jennifer M. Wolff

Department of Psychology: Dissertations, Theses, and Student Research

In this study, the neurobiological theory of adolescent decision making and risk taking and the dual-process decision making theory were tested in a sample of college students. Participants responded to questions in a survey about decision making style, socio-emotional processes, cognitive control processes, and deviant peers. The goals of the research were to test the relation between decision making processes (intuitive and deliberative) and risk behaviors, to test the potential overlap between intuitive and deliberative decision making as described in the more traditional dual-process models and the socio-emotional and cognitive control systems of the neurobiological model, and to extend the …

Mixed Methods Analysis Of School Wellness Programs In Nebraska And Indiana: A Descriptive Study, Jessica A. Robinson

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Schools have become one of the major fronts that the battle against childhood obesity is being fought. In 2004 the Child Nutrition Act passed by Congress required all school districts receiving funds for the National School Lunch Program to create a wellness policy. Since then there have been many research projects conducted to learn more about these policies, and their implementation as programs in the schools. Thus far, no such research has been done in Nebraska or Indiana. The purpose of this descriptive study is to describe how schools in Nebraska and Indiana are implementing wellness programs in their schools. …

Methamphetamine-Associated Psychosis, Kathleen M. Grant, Tricia D. Le Van, Sandra M. Wells, Ming Li, Scott F. Stoltenberg, Howard E. Gendelman, Gustavo Carlo, Rick A. Bevins

Department of Psychology: Faculty Publications

Methamphetamine (METH) is a frequent drug of abuse in U.S. populations and commonly associated with psychosis. This may be a factor in frequent criminal justice referrals and lengthy treatment required by METH users. Persecutory delusions and auditory hallucinations are the most consistent symptoms of METH-associated psychosis (MAP). MAP has largely been studied in Asian populations and risk factors have varied across studies. Duration, frequency and amount of use as well as sexual abuse, family history, other substance use, and co-occurring personality and mood disorders are risk factors for MAP. MAP may be unique with its long duration of psychosis and …

The Nurse In The School Health Office: Exploring Health Care In A Public School, Pamela A. Rademacher

College of Education and Human Sciences: Dissertations, Theses, and Student Research

To provide a high-quality education for all its students, schools must address a variety of needs that are related to physical, social and/or emotional health. School nurses are positioned to do that in the schools that they serve. Exploring how the school nurse intervenes to help children and their families to maintain a high level of health may contribute to an understanding of health care and academic achievement in the educational community. The purpose of this qualitative study is to gain an understanding of what a nurse does on a regular basis to provide health care to all children in …

Genome-Wide Microrna Profiling Of Mantle Cell Lymphoma Reveal A Distinct Subgroup With Poor Prognosis, Javeed Iqbal, Yulei Shen, Yanyan Liu, Kai Fu, Elaine Jaffe, Cuiling Liu, Zhongfeng Liu, Cynthia Lachel, Karen Deffenbacher, Timothy Greiner, Julie Vose, Sharathkumar Bhagavathi, Louis Staudt, Lisa Rimsza, Andreas Rosenwald, German Ott, Jan Delabie, Elias Campo, Rita Braziel, James Cook, Raymond Tubbs, Randy Gascoyne, James Armitage, Dennis Weisenburger, Timothy Mckeithan, Wing Chan

United States Public Health Resources

MicroRNA (miRNA) deregulation has been implicated in the pathogenesis of mantle cell lymphoma (MCL). Using a high-throughput quantitative real-time PCR platform, we performed miRNA profiling on cyclin D1- positive MCL (n=30) and cyclin D1-negative MCL (n=7) and compared them with small lymphocytic leukemia/lymphoma (SLL, n=12), aggressive B-cell lymphomas (n=138), normal B-cell subsets and stromal cells. We identified a 19-miRNA classifier which included six upregulated miRNAs (miR-135a, miR-708, miR-150, miR-363, miR-184, miR-342-5p) and 13 downregulated miRNAs, that was able to distinguish MCL from other aggressive lymphomas with >90% probability. Some of these upregulated miRNAs are highly expressed in naïve B-cells. MicroRNA …

Flowcell Systems For Single Molecule Detection, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi L. Reynolds, Pamela Sheaff

Biomedical Imaging and Biosignal Analysis Laboratory

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.

Single-Chain Vαvβ T Cell Receptors Function Without Mispairing With Endogenous Tcr Chains1, David H. Aggen, Adam S. Chervin, Thomas M. Schmitt, Boris Engels, Jennifer D. Stone, Sarah A. Richman, Kurt H. Piepenbrink, Brian M. Baker, Philip D. Greenberg, Hans Schreiber, David M. Kranz

Food for Health: Publications

Transduction of exogenous T cell receptor (TCR) genes into patients’ activated peripheral blood T cells is a potent strategy to generate large numbers of specific T cells for adoptive therapy of cancer and viral diseases. However, the remarkable clinical promise of this powerful approach is still being overshadowed by a serious potential consequence: mispairing of the exogenous TCR chains with endogenous TCR chains. These “mixed” heterodimers can generate new specificities that result in graft-versus-host reactions. Engineering TCR constant regions of the exogenous chains with a cysteine promotes proper pairing and reduces the mispairing, but, as we show here, does not …

Effects Of Leptin Replacement Alone And With Exendin-4 On Food Intake And Weight Regain In Weight-Reduced Diet-Induced Obese Rats, Roger Reidelberger, Alvin Haver, Prasanth K. Chelikani, Bettye Apenteng, Curtis Perriotte-Olson, Krista Anders, Sharalyn Steenson, James E. Blevins

United States Public Health Resources

Reidelberger R, Haver A, Chelikani PK, Apenteng B, Perriotte-Olson C, Anders K, Steenson S, Blevins JE. Effects of leptin replacement alone and with exendin-4 on food intake and weight regain in weight-reduced diet-induced obese rats. Am J Physiol Endocrinol Metab 302: E1576–E1585, 2012. First published April 17, 2012; doi:10.1152/ajpendo.00058.2012.—Weight loss in obese humans produces a relative leptin deficiency, which is postulated to activate potent orexigenic and energy conservation mechanisms to restrict weight loss and promote weight regain. Here we determined whether leptin replacement alone or with GLP-1 receptor agonist exendin-4 attenuates weight regain or promotes greater weight loss in weight-reduced …

Tailoring The Immune Response By Targeting C-Type Lectin Receptors On Alveolar Macrophages Using “Pathogen-Like” Amphiphilic Polyanhydride Nanoparticles, Ana V. Chavez-Santicoy, Rajarshi Roychoudhury, Nicola L.B. Pohl, Michael J. Wannemeuhler, Balaji Narasimhan, Amanda E. Ramer-Tait

Amanda Ramer-Tait Publications

C-type lectin receptors (CLRs) offer unique advantages for tailoring immune responses. Engagement of CLRs regulates antigen presenting cell (APC) activation and promotes delivery of antigens to specific intracellular compartments inside APCs for efficient processing and presentation. In these studies, we have designed an approach for targeted antigen delivery by decorating the surface of polyanhydride nanoparticles with specific carbohydrates to provide pathogen-like properties. Two conserved carbohydrate structures often found on the surface of respiratory pathogens, galactose and di-mannose, were used to functionalize the surface of polyanhydride nanoparticles and target CLRs on alveolar macrophages (AMϕ), a principle respiratory tract APC. Co-culture of …

Motivation And Loss Aversion In The Health Savings Account Paradigm, Michael Holtje

Department of Psychology: Dissertations, Theses, and Student Research

This investigation examined the role of “loss aversion” and consumers’ motivational orientations in the context of healthcare purchasing under the individual account paradigm and the defined benefit (insurance) paradigm. Specifically, this dissertation investigated (1) whether Health Savings Accounts (HSAs) encourage more prudent health care spending compared to traditional health insurance plans and (2) the impact of individuals' motivational preferences (either towards “gains” or towards “losses”) between decision tasks under each type of health plan.

Three experiments varied the type of healthcare plan that consumers had and the manner in which they received information (either as a “gain” or “loss”). The …

Assessment Of Nutrition Knowledge And Self-Efficacy Of Ncaa Athletes, Melissa M. Wallinga

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

BACKGROUND Little data has been collected on the nutrition knowledge and self efficacy of NCAA Athletes, and if Sports Nutrition Departments at Universities do positively affect the nutrition knowledge and self-efficacy of athletes. OBJECTIVES To determine and compare differences in nutrition knowledge and self-efficacy of athletes at two NCAA Division I Universities, one with a Sports Nutrition Department, and one without.

SETTING Participants completed a survey in person at their respective University.

PARTICIPANTS Male and female student athletes (n=327) from two Division I Universities, participating in men’s football, men’s wrestling, women’s soccer, or women’s swimming/diving.

RESULTS Approximately 42% of the …

Characterization Of Species C Human Adenovirus Serotype 6 (Ad6), Eric A. Weaver, Reeti Khare, Mathew L. Hillestad, Donna Palmer, Philip Ng, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Adenovirus serotype (Ad5) is the most studied Ad. Ad1, 2, and 6 are also members of species C Ad and are presumed to have biologies similar to Ad5. In this work, we have compared the ability of Ad1, 2, 5, and 6 to infect liver and muscle after intravenous and intramuscular injection. We found that Ad6 was surprisingly the most potent at liver gene delivery and that Ad1 and Ad2 were markedly weaker than Ad5 and 6. To understand these differences, we sequenced the Ad6 genome. This revealed that the Ad6 fiber protein is surprisingly three shaft repeats shorter than …

Heme Oxygenase-1 Regulates The Immune Response To Influenza Virus Infection And Vaccination In Aged Mice, Nathan W. Cummins, Eric A. Weaver, Shannon M. May, Anthony J. Croatt, Oded Foreman, Richard B. Kennedy, Gregory A. Poland, Michael A. Barry, Karl A. Nath, Andrew D. Badley

Nebraska Center for Virology: Faculty Publications

Underlying mechanisms of individual variation in severity of influenza infection and response to vaccination are poorly understood. We investigated the effect of reduced heme oxygenase-1 (HO-1) expression on vaccine response and outcome of influenza infection. HO-1-deficient and wild-type (WT) mice (kingdom, Animalia; phylum, Chordata; genus/species, Mus musculus) were infected with influenza virus A/PR/8/34 with or without prior vaccination with an adenoviral-based influenza vaccine. A genome-wide association study evaluated the expression of single-nucleotide polymorphisms (SNPs) in the HO-1 gene and the response to influenza vaccination in healthy humans. HO-1-deficient mice had decreased survival after influenza infection compared to WT mice (median …

A Pilot Study On The Attitudes Of Management Practices In Adults With Pku, Elizabeth L. Vlock

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Phenylketonuria (PKU) is a genetic disorder caused by the deficiency of the enzyme, phenylalanine hydroxylase. Phenylalanine accumulation in brain causes cognitive impairment and behavioral problems, which can be prevented by dietary restriction of phenylalanine. People with PKU have to maintain a highly restrictive low protein diet throughout their entire life. PKU is typically diagnosed at birth through newborn screening. In order for a patient to reach their optimal IQ and health status, they need to maintain their blood phenylalanine (phe) level within the recommended range of 60-360 µmol/L.

The purpose of this study is to investigate attitudes of adults diagnosed …

Sentence Recognition From Articulatory Movements For Silent Speech Interfaces, Jun Wang, Ashok Samal, Jordan R. Green, Frank Rudzicz

Department of Special Education and Communication Disorders: Faculty Publications

Recent research has demonstrated the potential of using an articulation-based silent speech interface for command-and-control systems. Such an interface converts articulation to words that can then drive a text-to-speech synthesizer. In this paper, we have proposed a novel near-time algorithm to recognize whole-sentences from continuous tongue and lip movements. Our goal is to assist persons who are aphonic or have a severe motor speech impairment to produce functional speech using their tongue and lips. Our algorithm was tested using a functional sentence data set collected from ten speakers (3012 utterances). The average accuracy was 94.89% with an average latency of …

Pathogenicity Of Treponema Denticola Wild-Type And Mutant Strain Tested By An Active Mode Of Periodontal Infection Using Microinjection, Jacques Izard, Hajime Sasaki, Ralph Kent

Department of Food Science and Technology: Faculty Publications

The available passive mode of periodontal infections in mice requires high efficiency of bacterial attachment and invasiveness and is not always suitable to test the pathogenicity of genetically engineered mutant strains. We developed an active mode of oral infection, using microinjection in the marginal gingiva of mice, to test the pathogenicity of a genetically engineered Treponema denticola mutant strain deficient in intermediate-like filaments, compared to the wild-type strain. This targeted mode of infection inoculates the bacterial strain to be tested directly at a lesion site (needle entry point) located at the future periodontal lesion site. The efficiency of T. …

Conformational Melding Permits A Conserved Binding Geometry In Tcr Recognition Of Foreign And Self Molecular Mimics, Oleg Y. Borbulevych, Kurt H. Piepenbrink, Brian M. Baker

Food for Health: Publications

Molecular mimicry between foreign and self antigens is a mechanism of T cell receptor cross reactivity and is thought to contribute to the development of autoimmunity. The αβ TCR A6 recognizes the foreign antigen Tax from the virus HTLV-1 when presented by the class I MHC HLA-A2. In a possible link with the autoimmune disease HAM/TSP, A6 also recognizes a self peptide from the neuronal protein HuD in the context of HLA-A2. We found here that the complexes of the HuD and Tax epitopes with HLA-A2 are close but imperfect structural mimics, and that in contrast with other recent structures …

Benchmarking Us Department Of Veterans Affairs Dermatologic Services: Results From A National Survey, L. Kendall Krause, Leilani Townsend, Michael L. Orser, Jennifer Mulhausen, Jodi Duke, Weston T. Waxweiler, Robert P. Dellavalle

United States Public Health Resources

Background: How well Department of Veterans Affairs (VA) dermatology services provide clinical care, medical education, and innovative research is a largely unexplored topic in the literature.

Objective: We sought to benchmark VA dermatology services by surveying VA dermatologists about their environment, resources, and the pros and cons of working in the VA.

Methods: Printed surveys were mailed to VA dermatologists and responses were compiled and analyzed.

Results: Of 105 dermatology services surveyed, 48% returned surveys completed by board-certified dermatologists (n = 50); 20 surveys completed by nondermatologists were excluded from the analysis. Most services trained …

Shining A New Light On Selective Polishing, Caren M. Barnes

College of Dentistry: Faculty Publications

The era of selective polishing is over. Polishing is an integral procedure provided by dental hygienists. Patients look forward to the resultant smooth, clean feeling, and dental hygienists must ensure that all patients are polished according to their individual needs—ensuring the safety of their teeth and restorations.

Identification And Analysis Of The Ige Binding By Parvalbumin And Other Potential Allergens In Different Fish And Frog Species, P. Lee, J. A. Nordlee, S. J. Koppelman, J. L. Baumert, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Rationale: Serological cross-reactivity to different fish and frog species is common among fish-allergic individuals.We examined the intra- and inter-individual diversity in IgE responses of fish-allergic subjects to various fish and frog species and identified novel allergens besides parvalbumin.

Methods: Sera from 38 subjects with a clinical history of fish allergy were analyzed for IgE-binding profiles to crude extracts of 26 raw fish and frog species, and purified cod and carp parvalbumin using IgE-immunoblotting. Sera of 7 subjects showing similar IgE-binding profiles in the IgEimmmunoblotting were pooled to identify potential allergens in pilchard, herring, cod, cusk, and rainbow trout using two-dimensional …

Two-Dimensional Blood Flow Velocity Estimation Using Apparent Speckle Pattern Angle Dependence On Scan Velocity, Tiantian Xu, Gregory R. Bashford

Biomedical Imaging and Biosignal Analysis Laboratory

An algorithm which measures the lateral component of blood flow velocity was developed in our previous studies based on the increase in speckle size due to relative motion between moving scatterers and spatial rate of scanner A-line acquisition (scan velocity). In this paper, the apparent dominant angle of the speckle pattern in a straight vessel was investigated and a new method of two-dimensional blood flow velocity estimation is introduced. Different scan velocities were used for data acquisition from blood flow traveling at an angle relative to the ultrasound beam. The apparent angle of the speckle pattern changes with different scan …

Monitoring Cerebral Hemodynamics With Transcranial Doppler Ultrasound During Cognitive And Exercise Testing In Adults Following Unilateral Stroke, Brian P. Watt, Judith M. Burnfield, Edward J. Truemper, Thad W. Buster, Gregory R. Bashford

Biomedical Imaging and Biosignal Analysis Laboratory

An observational study was performed as a preliminary investigation into the use of transcranial Doppler ultrasound (TCD) for recording cerebral hemodynamic changes during multiple tasks. TCD is a method of measuring cerebral blood flow (CBF) using ultrasound transducers in contact with the surface of the head. Using the maximum flow envelope of the Doppler spectrum returning from the middle cerebral artery (MCA), standard clinical flow indices can be calculated and displayed in real time providing information concerning perturbations in CBF and their potential cause. These indices as well as flow velocity measurements have been recognized as useful in measuring changes …

Augmented Input: The Effect Of Visuographic Supports On The Auditory Comprehension Of People With Chronic Aphasia, Sarah E. Wallace, Aimee R. Dietz, Karen Hux, Kristy S.E. Weissling

Department of Special Education and Communication Disorders: Faculty Publications

Background: Augmented input (AI), or the use of visuographic images and linguistic supports, is a strategy for facilitating the auditory comprehension of people with chronic aphasia. To date, researchers have not systematically evaluated the effects of various types of AI strategies on auditory comprehension.

Aims: The purpose of the study was to perform an initial evaluation of the changes in auditory comprehension accuracy experienced by people with aphasia when they received one type of AI. Specifically, the authors examined the effect four types of non-personalized visuographic image conditions on the comprehension of people with aphasia when listening to narratives.

Methods …

Trust In Government And Support For Governmental Regulation: The Case Of Electronic Health Records, Mitchel Herian, Nancy C. Shank, Tarik Abdel-Monem

University of Nebraska Public Policy Center: Publications

Background — This paper presents results from a public engagement effort in Nebraska, USA, which measured public opinions about governmental involvement in encouraging the use of electronic health records (EHRs).

Objective — We examine the role of trust in government in contributing to public support for government involvement in the development of EHR technologies. We hypothesize that trust in government will lead to support for federal and state governmental encouragement of the use of EHRs among doctors and insurance companies. Further, because individual experiences with health-care professionals will reduce perceptions of risk, we expect that support for governmental involvement will …

Diagnosis Of Porcine Teschovirus Encephalomyelitis In The Republic Of Haiti, Ming Y. Deng, Max Millien, Rodney Jacques-Simon, J. Keith Flanagan, Alexa J. Bracht, Consuelo Corrillo, Roger W. Barrette, Andrew Fabian, Fawzi Mohamed, Karen Moran, Jessica Rowland, Sabrina L. Swenson, Melinda Jenkins-Moore, Leo Koster, Bruce V. Thomsen, Gregory Mayr, Dave Pyburn, Paula Morales, John Shaw, Thomas Burrage, William White, Michael T. Mcintosh, Samia Metwally

Zoonotics and Wildlife Disease: Publications

In February and March 2009, approximately 1,500 backyard pigs of variable age became sick, and approximately 700 of them died or were euthanized in the Lower Artibonite Valley and the Lower Plateau of the Republic of Haiti. The main clinical sign was posterior ataxia followed by paresis and/or paralysis on the second or third day of illness. No gross lesions were observed at postmortem examinations. The morbidity and mortality were approximately 60% and 40%, respectively. Diagnostic samples (whole blood, brain, tonsil, lymph nodes, spleen, and lung) were negative for Classical swine fever virus and African swine fever virus. Porcine …

Cognitive Effects Of One Season Of Head Impacts In A Cohort Of Collegiate Contact Sport Athletes, Thomas W. Mcallister,, Laura A. Flashman, Arthur C. Maerlender, Richard M. Greenwald, Jonathan G. Beckwith, Tor D. Tosteson, Joe Crisco, Per Gunner Brolinson, Stefan Duma, Ann-Christine Duhaime, M. R. Grove, John H. Turco

Center for Brain, Biology, and Behavior: Faculty Publications

Objective: To determine whether exposure to repetitive head impacts over a single season negatively affects cognitive performance in collegiate contact sport athletes.

Methods: This is a prospective cohort study at 3 Division I National Collegiate Athletic Association athletic programs. Participants were 214 Division I college varsity football and ice hockey players who wore instrumented helmets that recorded the acceleration-time history of the head following impact, and 45 noncontact sport athletes. All athletes were assessed prior to and shortly after the season with a cognitive screening battery (ImPACT) and a subgroup of athletes also were assessed with 7 measures from a …

Association Between Acculturation And Breastfeeding Among Hispanic Women: Data From The Pregnancy Risk Assessment And Monitoring System, Indu B. Ahluwalia, Denise D’Angelo, Brian Morrow, Jill A. Mcdonald

United States Public Health Resources

Background: Breastfeeding rates are typically higher among Hispanic women; however, they vary by acculturation status in that those more acculturated are less likely to breastfeed than those who are less acculturated. This study examined the association between acculturation and breastfeeding behaviors using population-based data.

Methods: Data (N = 8942) from the Pregnancy Risk Assessment Monitoring System (PRAMS) were used for analysis. Acculturation status was determined using self-reported Hispanic ethnicity and the language in which the women responded to the PRAMS survey, either English or Spanish. Hispanic women who responded to the survey in Spanish were categorized as less acculturated than …

Most Physicians Were Eligible For Federal Incentives In 2011, But Few Had Ehr Systems That Met Meaningful-Use Criteria, Chun-Ju Hsiao, Sandra Decker, Esther Hing, Jane Sisk

United States Public Health Resources

As more physicians adopt electronic health record systems in their practices, policy interest is focusing on whether physicians are ready to meet the federal “meaningful use” criteria—a vital threshold to qualify for financial incentives. In our analysis of a 2011 nationally representative survey of office-based physicians, we found that 91 percent of physicians were eligible for Medicare or Medicaid meaningful-use incentives. About half of all physicians intended to apply. However, only 11 percent both intended to apply for the incentives and had electronic health record systems with the capabilities to support even two-thirds of the stage 1 core objectives required …

United States Registered Nurse Workforce Report Card And Shortage Forecast, Stephen P. Juraschek, Xiaoming Zhang, Vinoth K. Ranganathan, Vernon Lin

United States Public Health Resources

Registered nurses (RNs) play a critical role in health care delivery. With an aging US population, health care demand

is growing at an unprecedented pace. Using projected changes in population size and age, the authors developed

demand and supply models to forecast the RN job shortage in each of the 50 states. Letter grades were assigned

based on projected RN job shortage ratios. The number of states receiving a grade of “D” or “F” for their RN

shortage ratio will increase from 5 in 2009 to 30 by 2030, for a total national deficit of 918 232 (725 619 - …

Improving Outpatient Diabetes Care, Susan Kirsh, Michael Hein, Leonard Pogach, Gordon Schectman, Lauren Stevenson, Sharon Watts, Archana Radhakrishnan, John Chardos, David Aron

United States Public Health Resources

More than 20% of patients in the Veterans Health Administration (VHA) have diabetes; therefore, disseminating “best practices” in outpatient diabetes care is paramount. The authors’ goal was to identify such practices and the factors associated with their development. First, a national VHA diabetes registry with 2008 data identified clinical performance based on the percentage of patients with an A1c >9%. Facilities (n = 140) and community-based outpatient clinics (n = 582) were included and stratified into high, mid, and low performers. Semistructured telephone interviews (31) and site visits (5) were conducted. Low performers cited lack of teamwork between physicians and …