Medicine and Health Sciences Commons^™

The Use Of Fourier-Transformed Infrared Spectroscopy With An Infrared Biotyper To Establish Classifiers For Major Serogroups Of Moraxella Bovis Based On Spectral Profiles, Jordyn Bailey Schommer

Honors Theses

A classifier tool was created to identify major serogroups of Moraxella bovis based on spectral profiles collected from bacterial cells through the use of Fourier transformed infrared spectroscopy. M. bovis strains were selected from a group of previously sequenced and genotyped isolates, which provided the pilin sequence type for each isolate. The obtained spectroscopic profiles were analyzed through artificial neural networks to create a classifier tool that could identify the PilA type for unknown strains. Once a sufficient spectral library was obtained, the isolates were split into a training and a validation group, each containing spectra from isolates of five …

Early Intervention Within Orthodontics: The Sociological Aspects, Trenton Hammond

Honors Theses

Orthodontic treatment is one of the final phases of dental treatment. Its use is to provide aesthetics in some cases, yet the quality-of-life aspect that it can provide is greatly underestimated. Orthodontic care can provide proper function of teeth, improve facial structure, avoid tooth decay/loss, and improve gum health (AAO, 2024). Within this research the overarching goal is to understand the sociological aspects of families looking to start orthodontic care, what the reasons behind starting or not starting are, and providing information to the public about orthodontics that may be misunderstood or might push people away from searching for orthodontic …

A Comparative Analysis Of Hiv/Aids In France And The United States: Historical Context And Preventative Actions, Rebecca A. Liebsack

Honors Theses

The HIV/AIDS pandemic is the result of transmission of a zoonotic disease known as simian immunodeficiency virus. The pandemic has had profound social and economic consequences and continues to be present today. France and the United States’ response to the discovery of HIV will be compared and the impact that HIV/AIDS had on their countries and future responses. They had rather similar responses, however, the United States had a slower initial response compared to France. Both had similar takeaways such as aiming at improving prevention and utilizing tactics developed during the start of the pandemic like frequent testing and vaccines.

Toxicological Effects And Treatment Of Accidental Ingestion Of Simparica Trio® In Felis Catus: An Investigative Review, Aussia Stander

Honors Theses

Simparica TRIO^®, a canine endectocide manufactured by Zoetis, contains sarolaner, moxidectin, and pyrantel pamoate salt in an orally administered chewable tablet. These compounds are all toxic to both cats and dogs in sufficient amounts. As Simparica TRIO^® rises in popularity among pet owners, Felis catus (felines, cats) in multi-pet households (households with both cats and dogs) are at increased risk of overdosage and toxicosis from accidental ingestion of Simparica TRIO^® due to their smaller weight.

Simparica TRIO^® is not for feline use and little literature exists on the symptoms of and treatment for accidental ingestion …

Sarcopenia And The Importance Of Resistance Training And Protein-Rich Diets For Prevention Of Muscle Loss In Older Adults: A Literature Review And Informational Pamphlet, Madison Steele

Honors Theses

The growing prevalence of sarcopenia among older adults as well as youths has prompted further research aimed at developing effective preventative measures and treatment plans. Sarcopenia is often thought to occur solely due to aging, but several other factors contribute to progressive muscle loss. Previous research studies have found an effect of physical activity and dietary protein levels on the risk of developing sarcopenia as well as its onset age. This study aims to review existing knowledge in the field to compile an extensive list of sarcopenia causes and methods of prevention. The main causes identified in this review are …

Playful Progress: A Parent's Guide To Growth And Development, Gabriella N. Lawrey

Honors Theses

This thesis presents a creative project aimed at empowering parents to recognize developmental achievements, as well as address potential developmental delays in their children. Grounded in the principles of child development, this project focuses on creating age-appropriate activities that facilitate the identification of developmental milestones. By utilizing foundational resources in the field of child development, this toolkit of engaging activities has been curated to support parental understanding and observation. The primary objective of this applied knowledge project is to better equip parents with the vocabulary and tools to facilitate their child’s development. Throughout these activities, parents are encouraged to engage …

Narratives Of Reproductive Control In The American Eugenics Movement, Cassandra M. Provost

Honors Theses

In this paper, I will explore the eugenics movement as a pseudo-scientific political, social, and legal phenomenon which had a devastating historical impact on America’s most vulnerable women, as well as briefly discuss its residual effects on contemporary reproductive rights conversations, through the lens of literature. Using an interdisciplinary discourse and narrative analysis approach, I identify two distinct themes within the explored narratives: (1) the importance of a government’s attempt to override a person’s autonomy by destroying the person’s ability to reproduce, and (2) the impropriety of actions based on a negative attitude toward disabled or undesirable persons. In my …

Course Portfolio For Biochemistry 1: Structure And Metabolism (Bioc431), Didier Mena

UNL Faculty Course Portfolios

This benchmark portfolio encapsulates a comprehensive exploration aimed at enhancing the educational landscape of the BIOC431 course, a part of the general biochemistry course series (431 and 432). These courses are designed to offer a general introduction to the structure and function of cells in the body, along with their chemical reactions. Specifically, BIOC431 focuses on the structure, function, and metabolism of proteins, lipids, carbohydrates, and other major metabolic pathways. The three primary objectives addressed in this portfolio were the reevaluation of learning objectives, reassessment of assessment methods, and documentation of effective classroom strategies. Through background design, the learning objectives …

Site-Specific Space Use And Resource Selection By Black Vultures (Coragyps Atratus) In The Southeastern Usa, Betsy Evans, John S. Humphrey, Eric A. Tillman, Michael L. Avery, Bryan M. Kluever

USDA Wildlife Services: Staff Publications

North American populations of Black Vultures Coragyps atratus have increased and expanded their distribution in the southern and eastern USA. In conjunction with these patterns has been a rise in human–vulture conflicts. To improve our understanding of space use patterns and better inform management, we evaluated the movements of Black Vultures (n = 23) in the southeastern USA using a long-term GPS tracking database. Our specific objectives were to: (1) quantify home-range sizes in relation to season and geographical study location and (2) examine within-home-range resource selection to identify landscape and anthropogenic factors influencing roost and diurnal space use. …

Treatment With The Immunocontraceptive Vaccine, Gonacon, Induces Temporary Fertility Control In Free-Ranging Prairie Dog Populations In Colorado, Usa, Aaron B. Shiels, Jackson Runte, Emily W. Ruell, Douglas C. Eckery, Gary W. Witmer, Daniel J. Salkeld

USDA Wildlife Services: Staff Publications

Context

Prairie dogs (Cynomys spp.) are rodents enjoyed by some humans; yet, they cause crop and property damage, and carry zoonotic disease. Non-lethal control of prairie dogs is of interest in urban/suburban settings. The injectable immunocontraceptive vaccine GonaCon (active ingredient is gonadotropin-releasing hormone [GnRH]) has been shown to be effective at reducing fertility in equine and deer, and is a US EPA-registered vaccine for use in these ungulate species.

Aims

To conduct a replicated field study to test efficacy of GonaCon in black-tailed prairie dogs (C. ludovicianus), and if found to be efficacious, to help facilitate …

Comparison Of Ketamine-Xylazine, Butorphanol-Azaperone-Medetomidine, And Nalbuphine-Medetomidine-Azaperone For Raccoon (Procyon Lotor) Immobilization, Shylo R. Johnson, Christine K. Ellis, Chad Wickham, Molly R. Selleck, Amy T. Gilbert

USDA Wildlife Services: Staff Publications

Raccoons (Procyon lotor) are frequently handled using chemical immobilization in North America for management and research. In a controlled environment, we compared three drug combinations: ketamine-xylazine (KX), butorphanol-azaperone-medetomidine (BAM), and nalbuphinemedetomidine- azaperone (NalMed-A) for raccoon immobilization. In crossover comparisons, raccoons received a mean of the following: 8.66 mg/kg ketamine and 1.74 mg/kg xylazine (0.104 mL/kg KX); 0.464 mg/kg butorphanol, 0.155 mg/kg azaperone, and 0.185 mg/kg medetomidine (0.017 mL/kg BAM); and 0.800 mg/kg nalbuphine, 0.200 mg/kg azaperone, and 0.200 mg/kg medetomidine (0.020 mL/kg NalMed-A). Induction time was shortest with KX (mean6SE, 10.060.7 min) and longest with NalMed-A (13.061.3 min). …

Oral Rabies Vaccination Of Raccoons (Procyon Lotor) Across A Development Intensity Gradient In Burlington, Vermont, Usa, 2015–2017, Emily M. Beasley, Kathleen M. Nelson, Dennis Slate, Amy T. Gilbert, Frederick E. Pogmore, Richard B. Chipman, Amy J. Davis

USDA Wildlife Services: Staff Publications

Management of the raccoon rabies virus variant in North America is conducted primarily using oral rabies vaccination (ORV). When a sufficient proportion of the population is vaccinated (60%), rabies transmission can be eliminated. To date, ORV programs have successfully controlled and eliminated raccoon rabies in rural areas, but there has been less success in urban areas. We studied the proportions of rabies virus neutralizing antibodies (RVNA) in a raccoon (Procyon lotor) population during a 3-year ORV trial in developed areas of Burlington, Vermont, United States. We used a modified N-mixture model to estimate raccoon abundance, RVNA seroprevalence, and …

Recent Beak Evolution In North American Starlings After Invasion, Julia M. Zichello, Shelagh T. Deliberto, Paul Shrewsbury, Agnieszka A. Pierwola, Scott J. Werner

USDA Wildlife Services: Staff Publications

European starlings are one of the most abundant and problematic avian invaders in the world. From their native range across Eurasia and North Africa, they have been introduced to every continent except Antarctica. In 160 years, starlings have expanded into different environments throughout the world, making them a powerful model for understanding rapid evolutionary change and adaptive plasticity. Here, we investigate their spatiotemporal morphological variation in North America and the native range. Our dataset includes 1,217 specimens; a combination of historical museum skins and modern birds. Beak length in the native range has remained unchanged during the past 206 years, …

Assessing The Efficiency Of Local Rabies Vaccination Strategies For Raccoons (Procyon Lotor) In An Urban Setting, Guillaume Bastille-Rousseau, Nicole T. Gorman, Katherine M. Mcclure, Larissa Nituch, Tore Buchanan, Richard B. Chipman, Amy T. Gilbert, Kim M. Pepin

USDA Wildlife Services: Staff Publications

Raccoon rabies virus (RRV) has been managed using multiple vaccination strategies, including oral rabies vaccination and trap-vaccinate-release (TVR). Identifying a rabies vaccination strategy for an area is a nontrivial task. Vaccination strategies differ in the amount of effort and monetary costs required to achieve a particular level of vaccine seroprevalence (efficiency). Simulating host movement relative to different vaccination strategies in silico can provide a useful tool for exploring the efficiency of different vaccination strategies. We refined a previously developed individual-based model of raccoon movement to evaluate vaccination strategies for urban Hamilton, Ontario, Canada. We combined different oral rabies vaccination baiting …

Limitations Of Invasive Snake Control Tools In The Context Of A New Invasion On An Island With Abundant Prey, Shane R. Siers, Melia G. Nafus, Jereid E. Calaor, Rachel M. Volsteadt, Matthew S. Grassi, Megan Volsteadt, Aaron F. Collins, Patrick D. Barnhart, Logan T. Huse, Amy A. Yackel Adams, Diane L. Vice

USDA Wildlife Services: Staff Publications

In October 2020, a new population of invasive brown treesnakes (Boiga irregularis) was discovered on the 33-ha Cocos Island, 2.5 km off the south coast of Guam, United States. Cocos Island is a unique conservation resource, providing refuge for many lizards and birds, including endangered species, which were extirpated from mainland Guam by invasive predators including brown treesnakes. We sought to evaluate the usefulness of toxic baiting with acetaminophen-treated carrion baits and cage trapping, common tools for the control of brown treesnakes on mainland Guam, as potential eradication tools on Cocos Island. We evaluated multiple bait types and …

Instrumental Assessment Of Aero-Resistive Expiratory Muscle Strength Rehabilitation Devices, Angela M. Dietsch, Rahul Krishnamurthy, Kelsey Young, Steven M. Barlow

Department of Special Education and Communication Disorders: Faculty Publications

Purpose

Expiratory muscle strength training (EMST) is increasingly being used to treat voice, cough, and swallowing deficits in a wide range of conditions. However, a multitude of aero-resistive EMST models are commercially available, and the ab-sence of side-by-side comparative data interferes with clinicians’ ability to assess which model is best suited to a particular client’s needs. The primary aim of this research was to test and compare the pressure and flow parameters of six currently available EMST models to help inform clinical decision making.

Method

We identified and tested five devices of each of six different EMST models to generate …

I Am Not A Hero: Heroic Action Divorces The Hero From The Political Community, Ari Kohen, Brian Riches, Andre Sólo

Department of Political Science: Faculty Publications

Most people who perform a heroic act will, afterward, deny that their actions were heroic and claim that anyone would have done the same, even though that is demonstrably false (and, often, others were present who failed to act heroically at all). The literature on the psychology of heroism has never investigated why this is. This theoretical paper proposes an answer and seeks to provoke exploration of a previously unexplored topic. We note that people who undertake heroic action face a unique conflict: they embody their community’s highest values, while simultaneously breaking norms to stand apart from that community. We …

Providing Optimal Resident Sleep Hygiene In Assisted Living Communities; Opportunities For Design And Operation Teams To Collaborate And Share Their Unique Knowledge, Beverly Bj Miller

Architecture Masters of Science Program: Theses

Research is emerging relating sleep hygiene to disease pathogenesis including Parkinson's, dementia, Alzheimer’s disease and increased cardiovascular risk. The application of circadian science as it relates to human wellness has intensified as a result. The quality of sleep significantly impacts the wellness of residents living in long term care, however sleep hygiene research is lacking in assisted living (AL) communities.

The thesis question inquires; How can operation and design teams collaborate to optimize sleep health for residents in assisted living communities? The research methodology utilized a two-phase process. Phase I identified three AL communities as test sites, providing case studies …

Accelerated Evolution Of Sars-Cov-2 In Free-Ranging White-Tailed Deer, Dillon S. Mcbride, Sofya K. Garushyants, John Franks, Andrew F. Magee, Steven H. Overend, Devra Huey, Amanda M. Williams, Seth A. Faith, Ahmed Kandeil, Sanja Trifkovic, Lance Miller, Trushar Jeevan, Anami Patel, Jacqueline M. Nolting, Michael J. Tonkovich, J. Tyler Genders, Andrew J. Montoney, Kevin Kasnyik, Timothy J. Linder, Sarah N. Bevins, Julianna B. Lenoch, Jeffrey C. Chandler, Thomas J. Deliberto, Eugene V. Koonin, Marc A. Suchard, Philippe Lemey, Richard J. Webby, Martha I. Nelson, Andrew S. Bowman

USDA Wildlife Services: Staff Publications

The zoonotic origin of the COVID-19 pandemic virus highlights the need to fill the vast gaps in our knowledge of SARS-CoV-2 ecology and evolution in non-human hosts. Here, we detected that SARS-CoV-2 was introduced from humans into white-tailed deer more than 30 times in Ohio, USA during November 2021-March 2022. Subsequently, deer-to-deer transmission persisted for 2–8 months, disseminating across hundreds of kilometers. Newly developed Bayesian phylogenetic methods quantified how SARS-CoV-2 evolution is not only three-times faster in white-tailed deer compared to the rate observed in humans but also driven by different mutational biases and selection pressures. The long-term effect of …

Rapid Evolution Of A(H5n1) Influenza Viruses After Intercontinental Spread To North America, Ahmed Kandeil, Christopher Patton, Jeremy C. Jones, Trushar Jeevan, Walter N. Harrington, Sanja Trifkovic, Jon P. Seiler, Thomas Fabrizio, Karlie Woodard, Jasmine C. Turner, Jeri Carol Crumpton, Lance Miller, Adam Rubrum, Jennifer Debeauchamp, Charles J. Russell, Elena A. Govorkova, Peter Vogel, Mia Kim-Torchetti, Yohannes Berhane, David Stallknecht, Rebecca Poulson, Lisa Kercher, Richard J. Webby

USDA Wildlife Services: Staff Publications

Highly pathogenic avian influenza A(H5N1) viruses of clade 2.3.4.4b underwent an explosive geographic expansion in 2021 among wild birds and domestic poultry across Asia, Europe, and Africa. By the end of 2021, 2.3.4.4b viruses were detected in North America, signifying further intercontinental spread. Here we show that the western movement of clade 2.3.4.4b was quickly followed by reassortment with viruses circulating in wild birds in North America, resulting in the acquisition of different combinations of ribonucleoprotein genes. These reassortant A(H5N1) viruses are genotypically and phenotypically diverse, with many causing severe disease with dramatic neurologic involvement in mammals. The proclivity of …

An Investigation Of Match For Lossless Video Compression, Brittany Sullivan-Reicks

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

A new lossless video compression technique, Match, is investigated. Match uses the similarity between the frames of a video or the slices of medical images to find a prediction for the current pixel. A portion of the previous frame is searched to find a matching context, which is the pixels surrounding the current pixel, within some distance centered on the current location. The best distance to use for each dataset is found experimentally. The matching context refers to the neighborhood of w, nw, n, and ne, where the pixel in the previous frame with the closest matching context becomes the …

The Developmental Process For Adenoviral Vectored Canine Influenza Vaccines, Nicholas Jeanjaquet

School of Biological Sciences: Dissertations, Theses, and Student Research

Canine Influenza Virus (CIV) is a recently emerged branch of Influenza A virus that is highly infectious in dogs. The first strain was isolated in 2003, and it has quickly become endemic in areas with dense dog populations, most notably in Asia. The proximity of dogs to humans, along with their potential to serve as mixing vessels for reassortment, raises concern for possible zoonotic transmission and a potential human pandemic. Available vaccines are not frequently updated and struggle to prevent the spread of currently circulating strains. This highlights the need for a new and more effective vaccine. We outline the …

The Impact Of The Covid-19 Pandemic On The Well-Being Of People Incarcerated In United States Prisons, Kimberly Rivera

Department of Sociology: Dissertations, Theses, and Student Research

The COVID-19 pandemic has negatively impacted the population as a whole. However, the incarcerated population (which also experiences a variety of health disparities) has been disproportionately affected by the pandemic. Due to overcrowding, poor ventilation, and lack of resources, the incarcerated population already is at a heightened risk for negative health outcomes, made worse by the recent pandemic. To adapt to the rapidly changing conditions during the pandemic in 2020 and into 2022, new safety measures were implemented, but the unintended consequences associated with the implementation of these procedures have yet to be examined empirically. I conducted a qualitative content …

Pathogenicity In Chickens And Turkeys Of A 2021 United States H5n1 Highly Pathogenic Avian Influenza Clade 2.3.4.4b Wild Bird Virus Compared To Two Previous H5n8 Clade 2.3.4.4 Viruses, Mary J. Pantin-Jackwood, Erica Spackman, Christina Leyson, Sungsu Youk, Scott A. Lee, Linda M. Moon, Mia K. Torchetti, Mary L. Killian, Julianna B. Lenoch, Darrell R. Kapczynski, David E. Swayne, David L. Suarez

USDA Wildlife Services: Staff Publications

Highly pathogenic avian influenza viruses (HPAIVs) of subtype H5 of the Gs/GD/96 lineage remain a major threat to poultry due to endemicity in wild birds. H5N1 HPAIVs from this lineage were detected in 2021 in the United States (US) and since then have infected many wild and domestic birds. We evaluated the pathobiology of an early US H5N1 HPAIV (clade 2.3.4.4b, 2021) and two H5N8 HPAIVs from previous outbreaks in the US (clade 2.3.4.4c, 2014) and Europe (clade 2.3.4.4b, 2016) in chickens and turkeys. Differences in clinical signs, mean death times (MDTs), and virus transmissibility were found between chickens and …

Research Data Management Readiness At Uganda Cancer Institute, Edward Mukiibi, Joyce Bukirwa

Library Philosophy and Practice (e-journal)

The study explored research data management readiness at the Uganda Cancer Institute. Its objectives were to; establish the state of research data and the institutional readiness for research data management practices. The case study applied a survey method using a questionnaire modified from the Data Asset Framework and the Community Capability Model Framework. The respondents were 60 staff members at different professional levels purposively selected. The findings show massive data generated from clinical trials, and routine cancer clinics at the institute. The business processes are mainly manual except for the funded research projects which are hybrid. The existing data sets …

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy Gilbert, University Of Georgia, Guha Dharmarajan, Olin E. Rhodes Jr.

USDA Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern United States, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean …

Covid‑19‑Related Risk, Resilience, And Mental Health Among Mexican American Mothers Across The First Year Of The Pandemic, Amy L. Non, Elizabeth S. Clausing, Sandraluz Lara‑Cinisomo, Kimberly L. D’Anna Hernandez

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Background Latina mothers have been especially affected by the pandemic and historically exhibit high rates of depression and anxiety. However, few longitudinal studies have assessed the effect of the pandemic on this vulnerable population. We hypothesized that COVID-19-related stressors would associate with psychological distress among Latina mothers across the first year of the pandemic.

Methods We investigated COVID-19-related impact, stigma, and fears across two critical time points and changes in these measures in relation to changes in maternal anxiety and depression among mothers of Mexican descent living in Southern California (n=152). Surveys were administered within 5–16 weeks of …

Reduced Grey Matter Volume In Adolescents With Conduct Disorder: A Region‑Of‑Interest Analysis Using Multivariate Generalized Linear Modeling, Ru Zhang, R. James R. Blair, Karina S. Blair, Matthew Dobbertin, Jordan E. Pierce, Johannah Bashford‑Largo, Ahria J. Dominguez, Melissa Hatch, Sahil Bajaj

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Background Conduct disorder (CD) involves a group of behavioral and emotional problems that usually begins during childhood or adolescence. Structural brain alterations have been observed in CD, including the amygdala, insula, ventrolateral and medial prefrontal cortex, anterior cingulate cortex, and fusiform gyrus. The current study developed a multivariate generalized linear model (GLM) to differentiate adolescents with CD from typically developing (TD) adolescents in terms of grey matter volume (GMV).

Methods The whole‐brain structural MRI data were collected from 96 adolescents with CD (mean age = 16.188 ± 1.259 years; mean IQ = 104.292 ± 8.107 ; 63 males) and 90 …

Skin Lipids Alone Enable Conspecific Tracking In An Invasive Reptile, The Argentine Black And White Tegu Lizard (Salvator Merianae), M. Rockwell Parker, Eric A. Tillman, Lauren A. Nazarian, Megan L. Barlowe, Julianna M. Lincoln, Bryan M. Kluever

USDA Wildlife Services: Staff Publications

Locating potential mates in non-native habitats is one of the most important challenges faced by invasive vertebrate species. The Argentine black and white tegu lizard (Salvator merianae) is a major invasive reptile species in the contiguous United States and is rapidly expanding its range across Florida and the Southeast, in part due to inadequate management strategies and tools. Because a wide array of reptiles, especially squamates (snakes and lizards), have been well-studied for their reliance on chemical cues to locate conspecifics, our project aimed to isolate chemical cues from tegus and assess the ability of adult males and …

Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi

Biological Systems Engineering: Papers and Publications

High preweaning mortality (PWM) rates for piglets are a significant concern for the worldwide pork industries, causing economic loss and well-being issues. This study focused on identifying the factors affecting PWM, overlays, and predicting PWM using historical production data with statistical and machine learning models. Data were collected from 1,982 litters from the United States Meat Animal Research Center, Nebraska, over the years 2016 to 2021. Sows were housed in a farrowing building with three rooms, each with 20 farrowing crates, and taken care of by well-trained animal caretakers. A generalized linear model was used to analyze the various sow, …