Open Access. Powered by Scholars. Published by Universities.®

Medicine and Health Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

Articles 7051 - 7080 of 8080

Full-Text Articles in Medicine and Health Sciences

The Prevalance Of Tick Species (Ixodoidea) In Cattle, Sheep And Goats In The Burdur Region, Turkey, Bayram Ali̇ Yukari, Şi̇nasi̇ Umur Jan 2002

The Prevalance Of Tick Species (Ixodoidea) In Cattle, Sheep And Goats In The Burdur Region, Turkey, Bayram Ali̇ Yukari, Şi̇nasi̇ Umur

Turkish Journal of Veterinary & Animal Sciences

This study was conducted with the aim of identifying the tick species infesting cattle, sheep and goats and their seasonal activities in the Burdur area of Turkey. The study was carried out between 1 September 1999 and 31 August 2000 on animals owned by local farmers in Burdur and 14 surrounding villages. The study centres were visited twice a month. The ticks collected from animals and barns were brought to the laboratory and identified at species level by stereo-microscopic examination. A total of 756 cattle, 996 sheep and 698 goats were examined. During the study period, a total of 3280 …


The Possibility Of Using Goose Meat In The Production Of Salami, Ahmet Güner, Yusuf Doğruer, Gürkan Uçar, Hi̇lal Duygu Yörük Jan 2002

The Possibility Of Using Goose Meat In The Production Of Salami, Ahmet Güner, Yusuf Doğruer, Gürkan Uçar, Hi̇lal Duygu Yörük

Turkish Journal of Veterinary & Animal Sciences

The aim of this investigation was to compare some quality characteristics of goose-meat salami with those of turkey- and chicken-meat salami in order to determine its suitability for consumption and to able to use goose meat for meat product technology. The pH values of experimental samples were highest in the goose-meat salami and lowest in the turkey-meat salami. Moisture rates of samples produced with a mixture of two kinds of poultry meat were lower than those of the others. Salmonella, coliform group bacteria and yeast-mould microorganisms were negligible in the experimental salami samples. Salami samples produced only from goose meat …


The Effects Of Gonadotrophins On In Vitro Maturation Of Cat Oocytes, Mi̇that Evecen, Alper Baran, Çağatay Tek Jan 2002

The Effects Of Gonadotrophins On In Vitro Maturation Of Cat Oocytes, Mi̇that Evecen, Alper Baran, Çağatay Tek

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of FSH and LH hormones on in vitro development of cat oocytes. The ovaries from spayed cats were brought to the laboratory in +4 °C PBS solution two hours after being collected. The oocytes were recovered by slicing of ovaries and were separated into two groups: Group 1 (Control); TCM 199 + 5% FCS + 2.2 g/l NaHCO_3 + 0.23 mM Na Pyruvate; and Group 2 (Treatment); TCM 199 + 5% FCS + 2.2 g/l NaHCO_3 + 0.23mM Na Pyruvate + FSH (Sigma F-2293) (1 µg/ml) + LH (Sigma L-5269) …


Testis Characteristics In Ile De France (If) X Akkaraman (Ak)(G1) Male Lambs, Gürsel Dellal Jan 2002

Testis Characteristics In Ile De France (If) X Akkaraman (Ak)(G1) Male Lambs, Gürsel Dellal

Turkish Journal of Veterinary & Animal Sciences

This research was carried out on 125-day-old Ile de France (IF)X Akkaraman (AK) (G1) male lambs. In the research, the phenotypic parameters of some testis characteristics determined before and after slaughtering were calculated. The least-squares means of left and right testis diameters, left and right testis lengths, left and right testis volumes before and after slaughtering were found to be 3.7±0.09 cm and 4.2±0.12 cm; 3.9±0.10 cm and 4.2±0.11 cm; 6.6±0.17 cm and 7.4±0.21 cm; 6.9±0.17 cm and 7.4±0.17 cm; 51.7±2.76 cm^3 and 70.1±4.57 cm^3; 57.8±3.76 cm^3 and 87.4±7.86 cm^3, respectively. The least squares means of scrotal circumference before slaughtering …


The Effect Of Aflatoxin B1 On The Vitamin A Storage In The Livers Of Broiler Chicks, Berri̇n Salmanoğlu Jan 2002

The Effect Of Aflatoxin B1 On The Vitamin A Storage In The Livers Of Broiler Chicks, Berri̇n Salmanoğlu

Turkish Journal of Veterinary & Animal Sciences

The effect of AFB1 in feed on the liver and serum vitamin A and b-carotene levels and the ability of the liver to stor vitamin A in broiler chicks were investigated. Twenty day-old Ross broiler chicks were randomly allocated into 4 groups with 5 each in the control group, group I, group II, and group III. The chicks of the control group and group I were fed ad libitum on broiler chick growing feed. The chicks of group II and group III were fed ad libitum on feed with AFB1 (4 ppm/kg) feed. On the seventh day, vitamin A palmitate …


Seroprevalence Of Bovine Babesiosis Around Kayseri, Abdullah İnci̇, Ayşe Çakmak, K. Zafer Karaer, Şükran Di̇nçer, Fahri̇ Sayin, Anil İça Jan 2002

Seroprevalence Of Bovine Babesiosis Around Kayseri, Abdullah İnci̇, Ayşe Çakmak, K. Zafer Karaer, Şükran Di̇nçer, Fahri̇ Sayin, Anil İça

Turkish Journal of Veterinary & Animal Sciences

This study was carried out on cattle to detect the seroprevalence of babesiosis around Kayseri between July 1997 and January 1999. During this period a total of 191 cattle were examined for the Babesia species. Blood samples were collected from the cattle to obtain sera. During sampling, thick and thin blood smears were prepared from the punctured tail of each animal. Ticks were collected from the animals sampled. The sera were tested for the presence of antibodies to the Babesia species by IFAT. The blood smears were stained with 5% Giemsa's stain solution, and were examined microscopically with oil immersion. …


The Evaluation Of Renal Allo And Autotransplantation Results In Cats, Fatma Eser Özgenci̇l, Ömer Beşalti, Ahmet Özak, Nalan Karademi̇r, Jale Erdeğer, Betül Tanyel Jan 2002

The Evaluation Of Renal Allo And Autotransplantation Results In Cats, Fatma Eser Özgenci̇l, Ömer Beşalti, Ahmet Özak, Nalan Karademi̇r, Jale Erdeğer, Betül Tanyel

Turkish Journal of Veterinary & Animal Sciences

For the first time in Turkey, renal autotransplantation in 7 cats and renal allotransplantation in 7 cats having blood-crossmatch-compatible donors were performed. Immunosuppression was maintained by a prednisolone-cyclosporin combination in the renal allotransplantation group. All cats in the renal allotransplantation group died during the operation or between 3 and 72 h postoperatively. Two cats in the renal autotransplantation group survived approximately 2 years. Hyperacute or acute rejection findings were not encountered according to perioperative observations, laboratory findings or histopathological evaluations of renal allografts. The results of histopathological evaluations of renal allografts indicated acute tubular necrosis (ATN) findings caused by renal …


Colonization, Translocation And Antibody Response In Experimental Campylobacter Infection Of Chickens, Hakan Yardimci, Jale Erdeğer, Mehmet Akan, Murat Yildirim Jan 2002

Colonization, Translocation And Antibody Response In Experimental Campylobacter Infection Of Chickens, Hakan Yardimci, Jale Erdeğer, Mehmet Akan, Murat Yildirim

Turkish Journal of Veterinary & Animal Sciences

In this study we examined factors affecting the colonization of campylobacters in chick intestines, colonization times and elicited immune responses. In chicks, infected orally by minimal infective dose _{100} (MID_{100}), colonization in the intestines was observed to reach 100% during the 3-week period following C. jejuni and C. coli inoculation. When the SDS-PAGE protein profiles of the inoculated and isolated strains were examined similarities of 98.3% among C. jejuni strains and 98.5% among C. coli strains were determined. The results obtained from the ELISA tests performed with blood sera in the group infected with C. jejuni and in the control …


Transfer Of In Vitro Produced Sheep Embryos, Sema Bi̇rler, Serhat Pabuccuoğlu, Hatem Atalla, Serhat Alkan, Özen Banu Özdaş, Süleyman Bacinoğlu, Ümüt Ci̇ri̇t, İrem Zavar, Mehmet Emi̇n Cumhur Sönmez, İrfan Kamuran İleri̇ Jan 2002

Transfer Of In Vitro Produced Sheep Embryos, Sema Bi̇rler, Serhat Pabuccuoğlu, Hatem Atalla, Serhat Alkan, Özen Banu Özdaş, Süleyman Bacinoğlu, Ümüt Ci̇ri̇t, İrem Zavar, Mehmet Emi̇n Cumhur Sönmez, İrfan Kamuran İleri̇

Turkish Journal of Veterinary & Animal Sciences

The objective of the present study was to transfer sheep embryos produced in vitro to recipient ewes. Ovaries were taken from slaughtered Kıvırcık ewes and transferred to the laboratory in phosphate buffered saline (PBS) at 30-35 ºC. The cumulus-oocyte complexes were obtained by slicing and washing 1-6 mm diameter follicles and matured for 24 h in medium 199 supplemented with sodium pyruvate, follicle stimulating hormone (FSH), luteinizing hormone (LH) and 10% fetal calf serum (FCS) at 38.5 ºC under 5% CO_2 in humidified atmosphere. Fresh semen was collected from three Kıvırcık rams, pooled and prepared for in vitro fertilization by …


Seasonal Variation And Meat Composition Of Blue Crab (Callinectes Sapidus, Rathbun, 1896) Caught In İskenderun Bay, North-East Mediterranean, Canan Türeli̇, Ünal Erdem, Mehmet Çeli̇k Jan 2002

Seasonal Variation And Meat Composition Of Blue Crab (Callinectes Sapidus, Rathbun, 1896) Caught In İskenderun Bay, North-East Mediterranean, Canan Türeli̇, Ünal Erdem, Mehmet Çeli̇k

Turkish Journal of Veterinary & Animal Sciences

The seasonal variation and meat composition of blue crabs (Callinectes sapidus Rathbun, 1896) were investigated. Twenty (10 male, 10 female) crabs were used each season. The total weight of the samples was estimated and the ratios of crude protein, lipid, dry matter and crude ash were obtained from meat of the body and claws. Comparatively higher values of dry matter, crude ash and lipid were determined for the claw meat of males in winter. In the same season the crude protein and lipid composition of claws and crude ash of body meat were higher in females than other meat. The …


Molecular Cloning Of An \Alpha-Amylase Gene From Bacillus Subtilis Rskk246 And Its Expression In Escherichia Coli And In Bacillus Subtilis, Numan Özcan, Adem Altinalan, Mehmet Sai̇t Eki̇nci̇ Jan 2001

Molecular Cloning Of An \Alpha-Amylase Gene From Bacillus Subtilis Rskk246 And Its Expression In Escherichia Coli And In Bacillus Subtilis, Numan Özcan, Adem Altinalan, Mehmet Sai̇t Eki̇nci̇

Turkish Journal of Veterinary & Animal Sciences

Bacillus subtilis RSKK246 was found to produce approximately a 65-kDa \alpha-amylase enzyme. A gene was isolated encoding \alpha-amylase activity that corresponded to this size and was inserted into pUC18 plasmid which was transferred to Escherichia coli. An approximately 1.7kbp fragment, which contains a whole \alpha-amylase gene, was excised and inserted into pUB110 and then transferred into the different B. subtilis strains including RSKK246, RSKK243, RSKK244, YB886 and ORBAM. The a-amylase gene was cloned into the plasmids and expressed with its own promoter, and this promoter sequence seemed to function in the E. coli and in all B. subtilis strains. Specific …


Seroprevalance Of Babesia Ovis In Sheep In Malatya, Müni̇r Aktaş, Ali̇ Düzgün, Cahi̇t Babür Jan 2001

Seroprevalance Of Babesia Ovis In Sheep In Malatya, Müni̇r Aktaş, Ali̇ Düzgün, Cahi̇t Babür

Turkish Journal of Veterinary & Animal Sciences

This study was carried out in Malatya and vicinity to determine the seroprevalance of Babesia ovis in sheep. A total of 220 sheep sera were tested against B. ovis using the Enzyme-Linked Immunosorbent Assay (ELISA). In addition, peripheral blood smears from these sheep were prepared and examined for B. ovis. The ELISA test showed that 55.9% of sheep were seropositive for B. ovis infection. The infection rate was 49.2% in sheep which were 1-12 months old and 59% in sheep which were older than one year. In the microscopic examination of blood smears, four out of 220 sheep were found …


A Study On The Fattening Performance Of Tushin Lambs Subjected To Intensive Feeding, Mevlüt Karaoğlu, Muhli̇s Maci̇t, Ayhan Aksoy Jan 2001

A Study On The Fattening Performance Of Tushin Lambs Subjected To Intensive Feeding, Mevlüt Karaoğlu, Muhli̇s Maci̇t, Ayhan Aksoy

Turkish Journal of Veterinary & Animal Sciences

Some characteristics related with the fattening performance of Tushin lambs reared in the Research and Application Farm of Ataturk University and subjected to intensive fattening were determined. The inital fattening weight, final fattening weight, and total and daily weight gain during the fattening period were 19.71 kg, 38.07 kg, 18.35 kg and 203.48 g respectively. The feed conversion ratio (kg feed consumed/kg live weight gain) was 5.01. The effect of year on final fattening weight, total weight gain and average daily weight gain was found to be significant (p


Some Microbiological, Chemical And Physical Characteristics Of Pastırma Marketed In Erzurum, Muhammet İrfan Aksu, Mükerrem Kaya Jan 2001

Some Microbiological, Chemical And Physical Characteristics Of Pastırma Marketed In Erzurum, Muhammet İrfan Aksu, Mükerrem Kaya

Turkish Journal of Veterinary & Animal Sciences

In this study, 48 pastırma samples collected from 6 different retailers were examined. It is concluded that lactic acid bacteria, Micrococcus and Staphylococcus were found to be dominant in the flora. From the microbiologic analyses, Micrococcus/Staphylococcus, lactic acid bacteria, yeast and mould counts were determined as 4.00-7.45 log CFU/g, 3.75-7.89 logCFU/g,


Diagnosis And Treatment Of Intussusception In A Cow, Murat Dabak, Emi̇ne Ünsaldi, Yusuf Gül Jan 2001

Diagnosis And Treatment Of Intussusception In A Cow, Murat Dabak, Emi̇ne Ünsaldi, Yusuf Gül

Turkish Journal of Veterinary & Animal Sciences

An 8-year-old cow admitted to the Veterinary Faculty, Internal Medicine Clinic (University of Fırat, Elazığ/TURKEY), was diagnosed with intussusception, and the diagnosis was confirmed by experimental laparotomy. Intussusception was corrected by enterectomy and intestinal anastomosis. Defecation on the day following the operation indicated normal intestinal flow. The animal was kept under observation for ten days after the operation, and had a full recovery.


The Determination Of Ensiled Qualities Of Corn, Alfalfa And Sugar Beet Pulp And The Effects On Food Intake And Nutrient Digestibility When Fed To Lambs In Different Forms, Pinar Tatli, İ. Hali̇l Çerçi̇, Fuat Gürdoğan Jan 2001

The Determination Of Ensiled Qualities Of Corn, Alfalfa And Sugar Beet Pulp And The Effects On Food Intake And Nutrient Digestibility When Fed To Lambs In Different Forms, Pinar Tatli, İ. Hali̇l Çerçi̇, Fuat Gürdoğan

Turkish Journal of Veterinary & Animal Sciences

This study was conducted in two stages to determine the ensiled qualities of corn, alfalfa and sugar beet pulp, and the effects on food intake and nutrient digestibility of corn and alfalfa silages used alone, together and with sugar beet pulp silage in lambs. In the first stage, corn, alfalfa and sugar beet pulp were ensiled and silos were opened after 45 days. Silage samples were taken for determination of silage quality and the samples were analysed. Dry matter (DM) levels of corn, alfalfa and sugar beet pulp silages were found to be 28.56 and 31.44, 16.74%, respectively. The pH, …


Molecular Genetics Of Breast Cancer, Emel Ergül, Ali̇ Sazci Jan 2001

Molecular Genetics Of Breast Cancer, Emel Ergül, Ali̇ Sazci

Turkish Journal of Medical Sciences

Recent advances in molecular biology enable researchers to understand the basis of breast cancer much better and show that hereditery breast cancer may result from mutations on several specific gene loci including BRCA1, BRCA2, p53, ATM and PTEN. These genes are tumor supressor genes and although their functions are different, they are all involved in the maintenance of genomic stability after DNA damage. Mutations that impair the function of these genes may adversely affect the manner in which DNA damage is processed. It is likely that the risk of breast cancer development is increased through this mechanism. Moreover, there are …


Sandwich-Type Enzyme Immunoassay For Amyloid A4 Protein In Cerebrospinal Fluid From Patients With Head Trauma, İbrahi̇m Pi̇ri̇m Jan 2001

Sandwich-Type Enzyme Immunoassay For Amyloid A4 Protein In Cerebrospinal Fluid From Patients With Head Trauma, İbrahi̇m Pi̇ri̇m

Turkish Journal of Medical Sciences

The aim of this study was to design a simple method for detecting the amyloid A4 protein in the cerebrospinal fluid (CSF) of patients who had severe brain trauma caused by traffic or other accidents and to evaluate its diagnostic and prognostic value. For this purpose, a sandwich type ELISA assay was used. A4, a 4kDa protein of 39-43 amino acids, is a metabolic product of a membrane-spanning, 695-770 aa, precursor molecule, the b-amyloid precursor protein (b-APP). The polyclonal rabbit antibody against synthetic A4 protein (1-40 residues) was used as an immobilized antibody. A mouse monoclonal antibody against synthetic A4 …


Efficacy Of Some Disinfectants On Embryonated Eggs Of Toxocara Canis, Hasan Ayçi̇çek, Ender Yarsan, H. Oğuz Sarimehmetoğlu, Mehmet Tanyüksel, Nogay Gi̇rgi̇nkardeşler, Mustafa Özyurt Jan 2001

Efficacy Of Some Disinfectants On Embryonated Eggs Of Toxocara Canis, Hasan Ayçi̇çek, Ender Yarsan, H. Oğuz Sarimehmetoğlu, Mehmet Tanyüksel, Nogay Gi̇rgi̇nkardeşler, Mustafa Özyurt

Turkish Journal of Medical Sciences

The aim of this study was to evaluate the efficacy of various disinfectants in different concentrations against embryonated Toxocara canis eggs. For this purpose, 2.5%, 5%, 7.5%, and 10% iodine, 2% glutaraldehyde, 10% benzalkonium chloride, 7% sodium hypochloride, 1% potassium permanganate, 70% ethyl alcohol, 10% potassium hydroxide and 3% phenol solutions were used as the disinfectants. This study was performed both in vitro and in-vivo. In the in vitro experiment, T. canis eggs were treated with disinfectant solutions in different time intervals, and larval motility was observed under X400 magnification with an inverted microscope. Microscopic examinations revealed that T. canis …


Relationship Between Aging And Susceptibility To Oxidative Damage: An Assessment By Erythrocyte Membrane Proteins And Lipids, Ahmet Özaydin, İlhan Onaran, Kadi̇r Avşar, Vecdet Tezcan, Gönül Sultuybek Jan 2001

Relationship Between Aging And Susceptibility To Oxidative Damage: An Assessment By Erythrocyte Membrane Proteins And Lipids, Ahmet Özaydin, İlhan Onaran, Kadi̇r Avşar, Vecdet Tezcan, Gönül Sultuybek

Turkish Journal of Medical Sciences

Contradictory results have been reported from various studies regarding the effect of age on susceptibility to oxidative damage. Using different parameters, we carried out a comparative study on the erythrocytes and erythrocyte membranes of healthy elderly and young adults to determine how resistance to oxidative damage is affected by aging. Thin layer chromatography and polyacrylamide gel electrophoresis under denaturing conditions were used to estimate changes in membrane phospholipids and cytoskeletal proteins, respectively. Our results showed that these components of intact erythrocytes incubated with cumene hydroperoxide (CumOOH), a powerful oxidising agent, remained unchanged in both age groups. However, exposure of erythrocyte …


Seroprevalence Of Toxoplasmosis Gondii In Sheep In Yozgat, Turkey, Cahi̇t Babür, Berri̇n Esen, Gülay Vural Jan 2001

Seroprevalence Of Toxoplasmosis Gondii In Sheep In Yozgat, Turkey, Cahi̇t Babür, Berri̇n Esen, Gülay Vural

Turkish Journal of Veterinary & Animal Sciences

The sera collected from 152 sheep older than one year old sheep in various parts of Yozgat Province were tested for Toxoplasma gondii antibodies using the Sabin- Feldman dye test. Out of the 152 sera examined, 69 (45.4%) were seropositive at different dilutions (34 samples at 1/16, 21 at 1/64, 11 at 1/256 and 3 at 1/1024).


A Study On Direct And Indirect Effects Of Some Factors On Weaning Weight Of A Awassi Lambs, Mehmet Topal, Nuri̇ni̇sa Esenbuğa Jan 2001

A Study On Direct And Indirect Effects Of Some Factors On Weaning Weight Of A Awassi Lambs, Mehmet Topal, Nuri̇ni̇sa Esenbuğa

Turkish Journal of Veterinary & Animal Sciences

The correlation coefficients divided into direct and indirect effects among ewe weight in the mating period and lambing, birth weight and weaning age of lamb, and weaning weight of Awassi lambs raised in Erzurum conditions were determined in this study. In path analysis, the direct and indirect impact of birth weight and weaning age of lamb on weaning weight were found to be highly significant. Partial correlation and standardized partial regression coefficients among weaning weight, birth weight and weaning age of lamb were also observed to be highly significant.


Are Inflammatory Cytokines Potential Mediators Of Phenytoin-Induced Gingival Overgrowth?, Nurcan Buduneli̇, Gül Ati̇lla, Neci̇l Kütükçüler Jan 2001

Are Inflammatory Cytokines Potential Mediators Of Phenytoin-Induced Gingival Overgrowth?, Nurcan Buduneli̇, Gül Ati̇lla, Neci̇l Kütükçüler

Turkish Journal of Medical Sciences

The levels of interleukin-1beta, tumour necrosis factor-alpha, and interleukin 6 in gingival crevicular fluid of phenytoin-treated patients (from sites exhibiting overgrowth and sites not exhibiting overgrowth) and control subjects were investigated. Six epileptic patients exhibiting severe phenytoin-induced gingival overgrowth, 12 patients with chronic gingivitis and 11 subjects with clinically healthy periodontium were included in the study. The cytokine levels were analysed by enzyme-linked immunosorbent assay and expressed as ng/2 sites. Wilcoxon signed-rank test, Kruskall-Wallis, Mann-Whitney U test and Spearman rank correlation were used for statistical analysis of the data. Overgrown sites showed significantly higher levels of interleukin-1beta compared to the …


Production Of Monoclonal Antibody Specific For Human Hemoglobin, Selma Öztürk, Beyazit Çirakoğlu Jan 2001

Production Of Monoclonal Antibody Specific For Human Hemoglobin, Selma Öztürk, Beyazit Çirakoğlu

Turkish Journal of Medical Sciences

Hemoglobin is not normally present in sputum, urine or feces except in minute amounts. Demonstration of the presence of hemoglobin in these materials is an important diagnostic clue that usually implies bleeding within the respiratory, urinary or gastroentestinal tract, respectively. In this work, a hybridoma line secreting monoclonal antibody (Mab) highly specific for human hemoglobin A (hHbA) was generated by fusion of immunized BALB/c mouse splenic lymphocytes with non-secreting mouse myeloma cells followed by selection in hypoxanthine-aminopterin-thymidine (HAT) medium and the screening of culture fluids for hHb A affinity in ELISA. The Mab produced by MAM 2/3B6 is highly specific …


Flow Cytometric Analysis Of The Effects Of Methotrexate And Vepesid On The Hep-2 Cell Cycle, Ni̇zami̇ Duran, Adi̇l M. Allahverdi̇yev, Sali̇h Çeti̇ner Jan 2001

Flow Cytometric Analysis Of The Effects Of Methotrexate And Vepesid On The Hep-2 Cell Cycle, Ni̇zami̇ Duran, Adi̇l M. Allahverdi̇yev, Sali̇h Çeti̇ner

Turkish Journal of Medical Sciences

Objective: To determine the mechanism of action of Methotrexate and Vepesid on the HEp-2 cells isolated from human laryngeal cancer cells morphologically and flow cytometrically (G1, G2, S and PI). Materials and Methods: The HEp-2 continuous cell line was used. Cultivation of the cells was realized in EMEM medium with 10% fetal bovine serum at an atmosphere of 37ºC with 5% CO_2. Six different concentrations of Vepesid and Methotrexate were prepared by diluting with deionized water (5 µg/ml, 50 mg/ml, 500 µg/ml). The morphological and cell cycle parameters of HEp-2 cells were determined by inverted microscope and flow cytometer respectively. …


In Vitro Mic Determination Of Haemophilus Influenzae Strains Using E-Test, Ni̇lay Çöplü, Orhan Cem Aktepe, Selda Uluutku, Engi̇n Güvener Jan 2001

In Vitro Mic Determination Of Haemophilus Influenzae Strains Using E-Test, Ni̇lay Çöplü, Orhan Cem Aktepe, Selda Uluutku, Engi̇n Güvener

Turkish Journal of Medical Sciences

H. influenzae is a fastidious bacteria, so the selection of a reliable method for in-vitro susceptibility tests is particularly important. The E-test is a practical method for detecting in-vitro antimicrobial susceptibility of antibiotics by MIC values. This study was performed using the E-test for direct patient management and in order to obtain epidemiological data on H. influenzae. The detection of antimicrobial agent resistance among fifty-seven H. influenzae isolates from lower respiratory tract infections in childhood was studied by the E-test. The E-test was performed on HTM agar according to the instruction manual recommendations. The susceptibility ratios and the MIC90 values …


Effects Of Experimental Diabetes And Insulin Treatment On Rabbit Renal Morphology: A Quantitative And Qualitative Study, Hüseyi̇n Eray Bulut, Bi̇lge Onarlioğlu, Celal Kaloğlu, Öztürk Özdemi̇r, Semi̇h Ayan Jan 2001

Effects Of Experimental Diabetes And Insulin Treatment On Rabbit Renal Morphology: A Quantitative And Qualitative Study, Hüseyi̇n Eray Bulut, Bi̇lge Onarlioğlu, Celal Kaloğlu, Öztürk Özdemi̇r, Semi̇h Ayan

Turkish Journal of Medical Sciences

Diabetes causes metabolic defects and related vascular alterations such as atherosclerosis. These vascular alterations may also reflect the renal structural degeneration under the influence of diabetes. Therefore, the aim of the present study was to determine qualitatively and quantitatively the effect of experimental diabetes on rabbit renal cortical structure, and also to evaluate the reversal rate of the renal structural alterations by daily insulin treatment. Eighteen rabbits, bred and fed in standard laboratory conditions, were divided into 3 groups each containing 6 animals. While control group animals received no treatment, the rest were injected with a single dose of intravenous …


Analysis Of Ras Gene Mutation In Human Oral Tumours By Polymerase Chain Reaction And Direct Sequencing, Si̇nan Süzen, James M. Parry Jan 2001

Analysis Of Ras Gene Mutation In Human Oral Tumours By Polymerase Chain Reaction And Direct Sequencing, Si̇nan Süzen, James M. Parry

Turkish Journal of Medical Sciences

Genetic alterations in proto-oncogenes or tumour suppressor genes are believed to be one of the key events in the multistage process of carcinogenesis. Activating point mutations occurring in either one of the three ras proto-oncogene families are common genetic alterations in human and animal neoplasms. However, the mechanisms leading to oral cancer are not completely understood. Activation of the ras oncogene in oral carcinogenesis, although absent or rare in the western world, accounts for up to 35% of all malignancies in India and South Asia. Recognised aetiological agents of oral cancer include tobacco and alcohol. Tobacco-associated compounds such as nitrosamines …


Cytomorphologic Analysis Of Papanicolaou Stained Smears Collected From Floor Of The Mouth Mucosa In Patients With Or Without Oral Malignancy, Nur Mollaoğlu, Jonathan George Cowpe, Rita Walker Jan 2001

Cytomorphologic Analysis Of Papanicolaou Stained Smears Collected From Floor Of The Mouth Mucosa In Patients With Or Without Oral Malignancy, Nur Mollaoğlu, Jonathan George Cowpe, Rita Walker

Turkish Journal of Medical Sciences

Cytology has been found to be a reliable and accurate technique when used to diagnose early malignant transformation in areas of the body where visual examination is difficult or impossible. The factors initiating neoplastic growth are still not clearly understood. Thus, automated instruments, capable of objective and quantitative cell analysis, have been used in descriptive morphology, for the assessment of tumour cell heterogeneity. Smears were collected from a group of 33 patients presenting with squamous cell carcinoma. Nine females and 24 males presenting with squamous cell carcinoma of the floor of the mouth underwent quantitative cytological assessment. These slides were …


Igg Subclass Levels In Elf In Copd, Demet Karnak, Sumru Beder, Oya Kayacan, Levent Karaca Jan 2001

Igg Subclass Levels In Elf In Copd, Demet Karnak, Sumru Beder, Oya Kayacan, Levent Karaca

Turkish Journal of Medical Sciences

Objective: Deficiency of immunoglobulin (Ig) may be responsible for recurrent infections in chronic obstructive pulmonary disease (COPD). Our aim was to investigate the level of IgG subclass (IgGsc) in the epithelial lining fluid (ELF) and to try to find a possible relation between these levels and serum or bronchoalveolar lavage (BAL) IgGsc levels in COPD patients with recurrent acute exacerbation. Methods: Twenty-four clinically stable non-smoking COPD patients who had developed recurrent exacerbations three or four times a year and 17 individuals without COPD were enrolled as the experimental and control groups (Group I and II) respectively. The BAL procedure was …