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Articles 31 - 60 of 185
Full-Text Articles in Medicine and Health Sciences
Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z
Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to examine resistance against cadmium (Cd$^{+2}$), copper (Cu$^{+2}$), lead (Pb$^{+2}$), mercury (Hg$^{+2}$), and manganese (Mn$^{+2}$) in Escherichia coli isolates recovered from cattle stool using phenotypic (agar dilution) and genotypic [polymerase chain reaction (PCR)] methods. In addition, the isolates were genotyped via enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Escherichia coli was isolated and identified from the 100 stool samples that were examined in the study. It was determined phenotypically that all isolates were sensitive to mercury; 97% of them showed resistance to cadmium; 69% showed resistance to copper; 24% showed resistance to lead; …
Investigation Of Bovine Conceptus Galectins And The Effect Of Bovine Conceptus Secretory Proteins On The Endometrial Epithelial Transcriptome, Heather Lynn Baldwin
Investigation Of Bovine Conceptus Galectins And The Effect Of Bovine Conceptus Secretory Proteins On The Endometrial Epithelial Transcriptome, Heather Lynn Baldwin
Graduate Theses, Dissertations, and Problem Reports
Pregnancy loss in cattle is greatest in the first 30 days of gestation. Near d15 of gestation, bovine conceptuses elongate and express LGALS1, an immunosuppressive glycan-binding protein believed to be essential for implantation. However, other members of the bovine galectin family, including LGALS3, LGALS4, LGALS7, LGALS8, LGALS9 and a galectin-13-like gene (LGALS13L), also may be expressed by the elongating conceptus. Therefore, galectin expression in elongating, d16 bovine conceptuses and intercaruncular endometrium as well as elongating, d14 ovine conceptuses and intercaruncular endometrium was assessed by RT-qPCR. Angus heifer and Texel ewes underwent estrous cycle synchronization and either inseminated or not bred. …
Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok
Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok
Turkish Journal of Veterinary & Animal Sciences
A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, …
Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova
Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate the serological diagnostic potential of the Brucella recombinant outer membrane (rOMP25, rOMP31) and periplasmic proteins (rBP26, rSOD) in a comparative way using an indirect enzyme-linked immunosorbent assay (i-ELISA). Rabbit and/or mouse antibodies to Brucella whole cell and/or soluble protein preparations recognized all recombinant proteins used, which confirms the expression of target antigens in E. coli in active form. The recombinant proteins showed different antigenicity to antibodies of cattle kept on a brucellosis-affected (endemic) farm and/or a new focus of infection. Thus, the presence of anti-Brucella antibodies was confirmed by i-ELISA/rSOD in 79% …
Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar
Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar
Turkish Journal of Veterinary & Animal Sciences
A total of 20 domestic cattle (Bos taurus L.) and 15 water buffalo (Bubalis bubalis L.) skulls were analyzed in this study. All of the specimens belonged to female individuals. Using a total of 27 craniometric measurements from each of the skulls, 9 indices were calculated. Although there were statistically significant differences between the linear measurements of the skulls of both species, while calculating the indices with their ratios, the values of the measurements of the facial area were determinative in the craniology. Among these indices, the facial index 1 value was statistically significant (P < 0.01) in the comparison of these two species. On the other hand, while considering the orbit and foramen magnum measurements, in contrast with the transversal measurement, the height was more determinant for their index and form.
Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka
Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka
Turkish Journal of Veterinary & Animal Sciences
Anaplasmosis has become a major concern in the cattle industry throughout the world due to its great economic impact. The causative agents, Anaplasmaspecies, are primarily transmitted by ticks, occurring intracellularly within blood cells, with some species being zoonotic. In this study, the presence of Anaplasma spp. was investigated in the blood and milk of dairy cattle in the Philippines. Blood and milk samples were collected from 98 dairy cattle from selected farms in five provinces in the southern part of Luzon Island in the Philippines. After DNA extraction, a conventional PCR for the control gene actin was performed, followed by …
Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan
Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan
Animal and Food Sciences Faculty Publications
Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …
Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz
Electronic Theses and Dissertations
Effects of enzymatically hydrolyzed yeast (EHY) and supplementation frequency (FREQ) on immune parameters among beef cows and calves was evaluated. Eighty multiparous (parity = 4.2 ± 0.3) cows were fed a common brome hay-based diet (CP = 8.0 ± 0.17%). Cows were blocked by expected calving date and stratified by body condition score (BCS) before random assignment of treatment. Beginning 88 ± 5 d prior to parturition, cows were provided 1 kg daily or 3 kg every 3 d of a soybean hull-based supplement (CP = 34.0, % of DM) that contained 0 or 3 g/kg EHY. The daily supplement …
Detection And Characterization Of Extended-Spectrum Beta-Lactamase Genes(Bla$_{Tem}$ And Bla$_{Shv}$) Among Beta-Lactam-Resistant Fecal Coliforms Of Dairy Cattle From Chhattisgarh, India, Nidhi Rawat, Fateh Singh, Sadanand Devidas Hirpurkar, Chandrahas Sannat, Nitin Eknath Gade
Detection And Characterization Of Extended-Spectrum Beta-Lactamase Genes(Bla$_{Tem}$ And Bla$_{Shv}$) Among Beta-Lactam-Resistant Fecal Coliforms Of Dairy Cattle From Chhattisgarh, India, Nidhi Rawat, Fateh Singh, Sadanand Devidas Hirpurkar, Chandrahas Sannat, Nitin Eknath Gade
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to isolate the antimicrobial-resistant enterobacteria from cattle feces and to identify the extended-spectrum beta-lactamase (ESBL) and carbapenemase gene among them. A total of 70 cattle fecal swab samples were collected from Durg, Chhattisgarh, India, and cultured in nutrient broth. Bacterial isolation recovered 120 gram-negative bacteria. Antimicrobial susceptibility testing showed 90 (75%) coliform isolates (66 Escherichia coli, 20 Klebsiella sp., and two each of Enterobacter sp. and Citrobacter sp.) resistant to amoxicillin, amoxicillin-clavulanic acid, cefixime, ceftazidime, imipenem, or meropenem. Bacterial isolates showed the highest resistance rate for ceftazidime (61.6%), followed by cefixime and amoxicillin-clavulanic acid …
Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma
Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma
Turkish Journal of Veterinary & Animal Sciences
The present case report deals with the morphological (gross and histopathological) and histochemical characterization of Johne?s disease in a cow. The carcass was presented in a hidebound condition with sunken eyes and with a history of persistent and nonresponsive diarrhea. Necropsy revealed generalized gelatinization of the visceral and peripheral fat. Thorough gross examination unveiled major pathological changes in the digestive tract. The gallbladder was distended, with severely thickened walls and blood mixed with bile. The small intestine was severely congested and the mesenteric lymph nodes were enlarged. The opening of the small intestine showed marked thickening of the intestinal walls, …
Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska
Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska
Turkish Journal of Veterinary & Animal Sciences
The main aim of this study was to identify the association between the single nucleotide polymorphism (SNP) in the bovine interleukin-17A (IL-17A) gene and mastitis resistance. Additionally, the connection between the identified polymorphism and the somatic cell score (SCS) was tested. Two hundred and six Polish Holstein?Friesian cows were genotyped using the PCR-RFLP method. One SNP mutation (c.126G>A) was identified within the coding sequence of the analyzed gene. Results of logistic regression analysis displayed no significant association between the IL-17A genotype and clinical mastitis occurrence (Wald chi-square test = 0.74; P = 0.69). The GLM analysis demonstrated that genotype …
Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya
Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya
Turkish Journal of Veterinary & Animal Sciences
The purpose of the study was to use decision trees to predict increased levels of somatic cells in cow's milk. The material for the study comprised data collected in 2012-2014 from five farms in Poland equipped with an automatic milking system. Data on 803 Polish Holstein-Friesian cows were collected. In order to predict somatic cell count data mining techniques were used to build a graphical model of a decision tree. This study found that the most important factors to anticipate an elevated somatic cell count in cow's milk are milk conductivity, lactation stage, and lactation (primiparous and multiparous cow groups), …
Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni
Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni
Turkish Journal of Veterinary & Animal Sciences
Bovine parainfluenza virus type 3 (BPIV3) is a leading respiratory pathogen of cattle and is often implicated as a causative pathogen of bovine respiratory disease complex (BRDC). Three genotypes (BPIV3a-c) have been tentatively classified based on genetic and phylogenetic analyses. A BPIV3c isolate, designated NX49, was isolated using the Madin-Darby bovine kidney cell culture from cattle in western China, where feedlot cattle with severe respiratory disease symptoms were observed. Hemagglutination assay demonstrated that NX49 had low agglutination activity against guinea pig erythrocytes, whereas SD0835, the first BPIV3c strain reported in China, showed high agglutination activity. Phylogenic analysis indicated that NX49 …
Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut
Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut
Turkish Journal of Veterinary & Animal Sciences
Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3+ T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3+ T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. $\alpha$SMA positivity was mainly in the media of vessels. Smooth muscle …
A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi
A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi
Turkish Journal of Veterinary & Animal Sciences
Ticks are the main hematophagous arthropods and obligatory ectoparasites that are considered a vector of serious pathogens for animals and humans. The aims of the present survey were to assess the prevalence and diversity of ticks in cattle in Southeast Iran and to determine Coxiella burnetii in the ticks. From May 2014 to April 2015, 583 ticks were collected randomly from 257 cattle. Nested trans-PCR was performed to detect C. burnetii. The overall prevalence of hard tick infestation on cattle was 56.8%. In total, seven different species were identified: Rhipicephalus sanguineus sensu lato (32.6%), Hyalomma anatolicum (28.8%), Hyalomma excavatum (19%), …
Differences In Efficacy Between Gamithromycin, Tilmicosin, And Tulathromycin As Metaphylactic Treatments In High Risk Calves For Bovine Respiratory Disease, T. Miller, M. E. Hubbert, E. F. Schwandt, D. U. Thomson, C. D. Reinhardt
Differences In Efficacy Between Gamithromycin, Tilmicosin, And Tulathromycin As Metaphylactic Treatments In High Risk Calves For Bovine Respiratory Disease, T. Miller, M. E. Hubbert, E. F. Schwandt, D. U. Thomson, C. D. Reinhardt
Kansas Agricultural Experiment Station Research Reports
The cost of Bovine Respiratory Disease to the beef industry due to death, poorer conversions, and therapy is estimated to cost more than $3 billion per year. Identifying and mitigating Bovine Respiratory Disease in cattle can be difficult due to the increased susceptibility for Bovine Respiratory Disease in high risk cattle. One management option to minimize an outbreak of respiratory disease is the use of metaphylaxis, the mass treatment of a group of calves to reduce the incidence and adverse effects of respiratory disease on high risk animals. Criteria used to determine the necessity of metaphylactic treatment against Bovine Respiratory …
Comparison Of Four Ultrasonographic Approaches For The Diagnosis Ofacquired Reticular Diaphragmatic Hernia In Bovidae, Ashwani Kumar, Vandana Sangwan, Jitender Mohindroo, Narinder Singh Saini, Simrat Sagar Singh
Comparison Of Four Ultrasonographic Approaches For The Diagnosis Ofacquired Reticular Diaphragmatic Hernia In Bovidae, Ashwani Kumar, Vandana Sangwan, Jitender Mohindroo, Narinder Singh Saini, Simrat Sagar Singh
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to look at four ultrasonographic approaches that diagnose acquired reticular diaphragmatic hernia (RDH) using either left or right caudal thoracic approaches (4th/5th intercostal space) and compare them to those made more cranially (1st/2nd intercostal space). Each sonographic observation was made in 27 unsedated, standing animals (22 buffaloes and 5 cattle) that were surgically confirmed for RDH. Using the right caudal thoracic approach, the herniated reticulum was seen as medial to the thoracic wall at a depth of 3-5 cm and adjacent to the cardiac silhouette. With the left caudal thoracic approach, the herniated reticulum …
Twenty-Four Hour Holter Monitoring In Finishing Cattle Housed Outdoors, D. A. Frese, J. D. Thomason, C. D. Reinhardt, S. J. Bartle, D. N. Rethorst, G. H. Loneragan, E. F. Schwandt, D. U. Thomson
Twenty-Four Hour Holter Monitoring In Finishing Cattle Housed Outdoors, D. A. Frese, J. D. Thomason, C. D. Reinhardt, S. J. Bartle, D. N. Rethorst, G. H. Loneragan, E. F. Schwandt, D. U. Thomson
Kansas Agricultural Experiment Station Research Reports
Ambulatory electrocardiogram monitoring, in the form of Holter monitoring, has been used in human and veterinary medicine for decades as an aid in the diagnosis and determination of appropriate therapy of heart rhythm disturbances. Within veterinary medicine, Holter monitors have been primarily used in companion animal species, yet little attention has been given to food animal species. Moreover, the heart rhythm in clinically normal cattle fed high concentrate diets and housed outdoors in confined drylot facilities has not been previously reported. In order to properly identify pathologic arrhythmias in cattle, the normal rhythm and arrhythmia prevalence in healthy cattle should …
Talin2-Mediated Traction Force Drives Matrix Degradation And Cell Invasion, Lei Qi, Naser Jafari, Xiang Li, Zaozao Chen, Liqing Li, Vesa P. Hytönen, Benjamin T. Goult, Chang-Guo Zhan, Cai Huang
Talin2-Mediated Traction Force Drives Matrix Degradation And Cell Invasion, Lei Qi, Naser Jafari, Xiang Li, Zaozao Chen, Liqing Li, Vesa P. Hytönen, Benjamin T. Goult, Chang-Guo Zhan, Cai Huang
Markey Cancer Center Faculty Publications
Talin binds to β-integrin tails to activate integrins, regulating cell migration, invasion and metastasis. There are two talin genes, TLN1 and TLN2, encoding talin1 and talin2, respectively. Talin1 regulates focal adhesion dynamics, cell migration and invasion, whereas the biological function of talin2 is not clear and, indeed, talin2 has been presumed to function redundantly with talin1. Here, we show that talin2 has a much stronger binding to β-integrin tails than talin1. Replacement of talin2 Ser339 with Cys significantly decreased its binding to β1-integrin tails to a level comparable to that of talin1. Talin2 localizes at invadopodia and is indispensable …
Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies
Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies
Animal, Dairy, and Veterinary Science Faculty Publications
Trophoblast cells from bovine somatic cell nuclear transfer (SCNT) conceptuses express major histocompatibility complex class I (MHC-I) proteins early in gestation, and this may be one cause of the significant first-trimester embryonic mortality observed in these pregnancies. MHC-I homozygous-compatible (n = 9), homozygous-incompatible (n = 8), and heterozygous-incompatible (n = 5) SCNT pregnancies were established. The control group consisted of eight pregnancies produced by artificial insemination. Uterine and placental samples were collected on Day 35 ± 1 of pregnancy, and expression of MHC-I, leukocyte markers, and cytokines were examined by immunohistochemistry. Trophoblast cells from all SCNT pregnancies expressed MHC-I, while …
Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner
Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner
Veterinary Diagnostic Laboratory Faculty Publications
Microcystins are acute hepatotoxins of increasing global concern in drinking and recreational waters and are a major health risk to humans and animals. Produced by cyanobacteria, microcystins inhibit serine/threonine protein phosphatase 1 (PP1). A cost-effective PP1 assay using p-nitrophenyl phosphate was developed to quickly assess water and rumen content samples. Significant inhibition was determined via a linear model, which compared increasing volumes of sample to the log-transformed ratio of the exposed rate over the control rate of PP1 activity. To test the usefulness of this model in diagnostic case investigations, samples from two veterinary cases were tested. In August …
Molecular Identification Of Bovine Papillomaviruses In Dairy And Beef Cattle: First Description Of Xi- And Epsilonpapillomavirus In Turkey, Veysel Soydal Ataseven, Özgür Kanat, Yaşar Ergün
Molecular Identification Of Bovine Papillomaviruses In Dairy And Beef Cattle: First Description Of Xi- And Epsilonpapillomavirus In Turkey, Veysel Soydal Ataseven, Özgür Kanat, Yaşar Ergün
Turkish Journal of Veterinary & Animal Sciences
In the current study, 23 papilloma/tumor-like samples obtained from cattle having clinical lesions and 9 blood samples collected from healthy-appearing cattle in Turkey were examined for bovine papillomavirus (BPV) DNA using the degenerate primers FAP59/64, and the different types of BPV were distinguished by type-specific primer sets. Furthermore, histopathological studies of papillomavirus were performed. A total of 7 BPV types (BPVs 1, 2, 3, 4, 6, 8, 9), including genera Deltapapillomavirus, Xipapillomavirus, and Epsilonpapillomavirus, were identified. In all samples, BPV-1 was the most common genotype (90.6%). Overall, coinfections were determined in 26 of the examined samples (81.3%), and coinfection with …
Cla And Epa Inhibit Lps-Induced Prostaglandin Release From Bovine Endometrial Cells Through An Nf-?B-Dependent Signaling Mechanism, Lokenga Badinga, Mehmet Şükrü Gülay, Alan Ealy
Cla And Epa Inhibit Lps-Induced Prostaglandin Release From Bovine Endometrial Cells Through An Nf-?B-Dependent Signaling Mechanism, Lokenga Badinga, Mehmet Şükrü Gülay, Alan Ealy
Turkish Journal of Veterinary & Animal Sciences
Although their action in antagonizing arachidonic acid metabolism is a key antiinflammatory effect of conjugated linoleic acid (CLA) and omega-3 polyunsaturated fatty acids (n-3 PUFA), these molecules have other antiinflammatory effects that might occur upstream of altered eicosanoid production. We examined the effects of two CLA isomers (c9,t11 and t10,c12 isomers), n-3 PUFA (EPA and DHA), and ΚNF-ΚB inhibitor (ammonium pyrrolidine dithiocarbamate (PDTC)) on lipopolysaccharide (LPS)-induced secretion of prostaglandins (PGE2 and PGF2a) by bovine endometrial (BEND) cells. LPS increased PGE2 and PGF2a concentrations in BEND cell-conditioned media in a concentration- and time-dependent manner. The c9,t11 CLA isomer and EPA decreased …
Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem
Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem
Turkish Journal of Veterinary & Animal Sciences
In this study, the presence of endosymbiotic Wolbachia bacteria was determined by immunohistochemical methods in 8 out of 43 paraffin-embedded bovine aortic tissues that were macroscopically and microscopically diagnosed with aortic onchocerciasis in the archive of the Harran University Faculty of Veterinary Medicine's Department of Pathology. By means of the enzyme histochemical and immunohistochemical staining methods, it was ascertained that the tryptase-containing subtype of mast cells (MCT) was involved in the inflammatory response in the bovine aortae with onchocerciasis. The aim of this study was to elucidate the pathogenesis of aortic onchocerciasis lesions caused by Onchocerca armillata in Turkey and …
Mycoplasma Infections In Dairy Cattle Farms In Turkey, Zafer Sayin, Asli Sakmanoğlu, Uçkun Sai̇t Uçan, Ali̇ Uslu, Hasan Hüseyi̇n Hadi̇mli̇, Zeki̇ Aras, Özgür Özdemi̇r, Osman Ergani̇ş
Mycoplasma Infections In Dairy Cattle Farms In Turkey, Zafer Sayin, Asli Sakmanoğlu, Uçkun Sai̇t Uçan, Ali̇ Uslu, Hasan Hüseyi̇n Hadi̇mli̇, Zeki̇ Aras, Özgür Özdemi̇r, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
Mycoplasmas cause the most severe and economically costly diseases of cattle throughout the world. In this study, Mycoplasma species were isolated from calves and cows with suspected mycoplasmosis in Holstein dairy cattle farms within 7 geographical regions of Turkey between May 2010 and December 2015. Mycoplasma infections were positive in 17 (80.9%) of 21 dairy cattle farms and the overall percentage was calculated as 32.1%. The highest isolation rate occurred in the Southeastern Anatolia Region (42.8%), and the lowest was observed in the Mediterranean Region (19.6%). In total, 172 Mycoplasma spp. were isolated from samples. Using PCR analysis, 149 (87.6%) …
Comparison Of Hematological Parameters Of Brucella-Seronegative Naturallyinfected Cattle With Bvdv, Bhv-1, And Bhv-4, Muhammet Eren Aslan, Ahmet Kürşat Azkur, Emel Biyikli, Serkal Gazyağci, İbrahi̇m Sözdutmaz
Comparison Of Hematological Parameters Of Brucella-Seronegative Naturallyinfected Cattle With Bvdv, Bhv-1, And Bhv-4, Muhammet Eren Aslan, Ahmet Kürşat Azkur, Emel Biyikli, Serkal Gazyağci, İbrahi̇m Sözdutmaz
Turkish Journal of Veterinary & Animal Sciences
Abortion, neonatal deaths, and infertility cause important production losses in the livestock industry. Many viral infections are responsible for changes in hematological values of animals. This study aimed to find whether there is any difference in blood parameters of cattle that are naturally infected with BVDV, BHV-1, and BHV-4 and Brucella-seronegative. For this purpose, 401 whole-blood samples collected from cattle were analyzed by ELISA and PCR/RT-PCR to detect specific antibodies and antigens for BVDV, BHV-1, and BHV-4. Hematological analysis and results were compared between naturally infected BVDV, BHV-1, and BHV-4 groups and a control group. Results showed a statistically significant …
The Effect Of Dgat1 Polymorphism On Milk Production Traits In Dairycows Depending On Environmental Temperature, Jolanta Komisarek, Mateusz Kolenda
The Effect Of Dgat1 Polymorphism On Milk Production Traits In Dairycows Depending On Environmental Temperature, Jolanta Komisarek, Mateusz Kolenda
Turkish Journal of Veterinary & Animal Sciences
It has long been known that a hot environment negatively influences milk production in cattle. Although molecular mechanisms underlying this phenomenon to a great extent remain to be recognized, they include alterations in lipid metabolism. DGAT1 plays a key role in the synthesis of triacylglycerols and polymorphism of this gene has been proven to have a pronounced influence on milk production. The aim of this study was to establish if the effect of DGAT1:p.K232A on milk-related traits is dependent on environmental thermal conditions. The analysis, performed on 468 cows of the Polish Holstein-Friesian breed, showed that allele DGAT1:K232 significantly increased …
Summer-Long Grazing Of High Vs. Low Endophyte (Neotyphodium Coenophialum)-Infected Tall Fescue By Growing Beef Steers Results In Distinct Temporal Blood Analyte Response Patterns, With Poor Correlation To Serum Prolactin Levels, Joshua J. Jackson, Merlin D. Lindemann, James A. Boling, James C. Matthews
Summer-Long Grazing Of High Vs. Low Endophyte (Neotyphodium Coenophialum)-Infected Tall Fescue By Growing Beef Steers Results In Distinct Temporal Blood Analyte Response Patterns, With Poor Correlation To Serum Prolactin Levels, Joshua J. Jackson, Merlin D. Lindemann, James A. Boling, James C. Matthews
Animal and Food Sciences Faculty Publications
Previously, we reported the effects of fescue toxicosis on developing Angus-cross steer growth, carcass, hepatic mRNA, and protein expression profiles of selected serum proteins, and blood clinical and chemical profiles, after summer-long grazing (85 days) of high endophyte (HE)- vs. low endophyte (LE)-infected fescue pastures. We now report the temporal development of acute, intermediate, and chronic responses of biochemical and clinical blood analytes determined at specified time intervals (period 1, day 0–36; period 2, day 37–58; and period 3, day 59–85). Throughout the trial, the alkaloid concentrations of the HE forage was consistently 19–25 times greater (P ≤ 0.002) …
Investigations Into The Cross-Infectivity Of Nematode Parasites Of Cattle And Sheep, Eva Marie Wray
Investigations Into The Cross-Infectivity Of Nematode Parasites Of Cattle And Sheep, Eva Marie Wray
Graduate Theses and Dissertations
The existence of anthelmintic resistance is not singular in parasite species, host species or geographic region. It has become a problem of worldly stature, especially when considering sheep nematodes. Recommendations have been modified from chemical-based control to multipart, managerial intervention. Compared with nematodes of cattle, the worldwide resistance of sheep nematodes to anthelmintic remedies is much more pronounced and urgent. As the resistance to essentially all anthelmintic classes approaches a cautionary level, the implementation of non-chemical controls has grown increasingly essential. Six-6 to 8 month old Holstein steer calves, six-5 month old Katahdin ram lambs and eight-5 month old Suffolk …
Exogenous Administration Of Lipids To Steers Alters Aspects Of The Innate Immune Response To Endotoxin Challenge, Nicole C. Burdick Sanchez, Jeffery A. Carroll, Janet R. Donaldson, Joe O. Buntyn, Ty B. Schmidt
Exogenous Administration Of Lipids To Steers Alters Aspects Of The Innate Immune Response To Endotoxin Challenge, Nicole C. Burdick Sanchez, Jeffery A. Carroll, Janet R. Donaldson, Joe O. Buntyn, Ty B. Schmidt
Department of Animal Science: Faculty Publications
This study examined the effects of increasing energy availability from both dextrose and lipid treatments on the proinflammatory response to LPS in Holstein steers. Steers were randomly assigned to one of three groups: saline at 0.5 ml/ kg body weight (Control) or 50% dextrose [0.5 ml/kg body weight (Dextrose) to mimic calm cattle’s response to LPS] administered immediately prior to LPS (0.5 mg/kg body weight at 0 h) or continuous lipid emulsion infusion from –1 to 6 h [Intralipid 20% (Baxter, Deerfield, IL USA); 0.5 ml/kg/hr (Lipid) to mimic temperamental cattle]. Concentrations of non-esterified fatty acids (NEFA) were greater in …