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Full-Text Articles in Medicine and Health Sciences
Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan
Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan
Animal and Food Sciences Faculty Publications
Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …
Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz
Electronic Theses and Dissertations
Effects of enzymatically hydrolyzed yeast (EHY) and supplementation frequency (FREQ) on immune parameters among beef cows and calves was evaluated. Eighty multiparous (parity = 4.2 ± 0.3) cows were fed a common brome hay-based diet (CP = 8.0 ± 0.17%). Cows were blocked by expected calving date and stratified by body condition score (BCS) before random assignment of treatment. Beginning 88 ± 5 d prior to parturition, cows were provided 1 kg daily or 3 kg every 3 d of a soybean hull-based supplement (CP = 34.0, % of DM) that contained 0 or 3 g/kg EHY. The daily supplement …
Detection And Characterization Of Extended-Spectrum Beta-Lactamase Genes(Bla$_{Tem}$ And Bla$_{Shv}$) Among Beta-Lactam-Resistant Fecal Coliforms Of Dairy Cattle From Chhattisgarh, India, Nidhi Rawat, Fateh Singh, Sadanand Devidas Hirpurkar, Chandrahas Sannat, Nitin Eknath Gade
Detection And Characterization Of Extended-Spectrum Beta-Lactamase Genes(Bla$_{Tem}$ And Bla$_{Shv}$) Among Beta-Lactam-Resistant Fecal Coliforms Of Dairy Cattle From Chhattisgarh, India, Nidhi Rawat, Fateh Singh, Sadanand Devidas Hirpurkar, Chandrahas Sannat, Nitin Eknath Gade
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to isolate the antimicrobial-resistant enterobacteria from cattle feces and to identify the extended-spectrum beta-lactamase (ESBL) and carbapenemase gene among them. A total of 70 cattle fecal swab samples were collected from Durg, Chhattisgarh, India, and cultured in nutrient broth. Bacterial isolation recovered 120 gram-negative bacteria. Antimicrobial susceptibility testing showed 90 (75%) coliform isolates (66 Escherichia coli, 20 Klebsiella sp., and two each of Enterobacter sp. and Citrobacter sp.) resistant to amoxicillin, amoxicillin-clavulanic acid, cefixime, ceftazidime, imipenem, or meropenem. Bacterial isolates showed the highest resistance rate for ceftazidime (61.6%), followed by cefixime and amoxicillin-clavulanic acid …
Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma
Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma
Turkish Journal of Veterinary & Animal Sciences
The present case report deals with the morphological (gross and histopathological) and histochemical characterization of Johne?s disease in a cow. The carcass was presented in a hidebound condition with sunken eyes and with a history of persistent and nonresponsive diarrhea. Necropsy revealed generalized gelatinization of the visceral and peripheral fat. Thorough gross examination unveiled major pathological changes in the digestive tract. The gallbladder was distended, with severely thickened walls and blood mixed with bile. The small intestine was severely congested and the mesenteric lymph nodes were enlarged. The opening of the small intestine showed marked thickening of the intestinal walls, …
Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska
Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska
Turkish Journal of Veterinary & Animal Sciences
The main aim of this study was to identify the association between the single nucleotide polymorphism (SNP) in the bovine interleukin-17A (IL-17A) gene and mastitis resistance. Additionally, the connection between the identified polymorphism and the somatic cell score (SCS) was tested. Two hundred and six Polish Holstein?Friesian cows were genotyped using the PCR-RFLP method. One SNP mutation (c.126G>A) was identified within the coding sequence of the analyzed gene. Results of logistic regression analysis displayed no significant association between the IL-17A genotype and clinical mastitis occurrence (Wald chi-square test = 0.74; P = 0.69). The GLM analysis demonstrated that genotype …