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A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir Jan 2023

A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir

Turkish Journal of Veterinary & Animal Sciences

In vitro embryo production (IVP) can help alleviating reproductive difficulties in cattle and buffalo. This study compared qualitative and quantitative features of native cattle and buffalo ovaries and oocytes to understand them comprehensively. In addition, it was conducted to pick the ideal ovaries for oocyte collection and to assess either maturation affects oocyte diameter or not. The ovaries were collected from the local slaughterhouses of Dhaka city, Bangladesh. Next, the quantitative parameters of ovaries were measured, the follicular fluid was aspired, the oocytes were classified, and the diameters of oocytes were determined in both pre and postmaturation. Here, the results …


Effect Of Lipid-Enriched Diet Associated With Bovine Recombinant Somatotropin On Superovulation Response And Embryo Recovery In Heifers, Richelle Matos Oliveira, Anselmo Domingos Ferreira Santos, Francisco Palma Rennó, Camilla Mendonça Silva Jan 2023

Effect Of Lipid-Enriched Diet Associated With Bovine Recombinant Somatotropin On Superovulation Response And Embryo Recovery In Heifers, Richelle Matos Oliveira, Anselmo Domingos Ferreira Santos, Francisco Palma Rennó, Camilla Mendonça Silva

Turkish Journal of Veterinary & Animal Sciences

The current study aimed to evaluate the effects of soybean oil supplementation associated with the administration of bovine recombinant somatotropin (rBST) on the population of ovarian follicles, standardization of both the superovulation response and embryo recovery in heifers. We used 40 Holstein heifers submitted to a 2 × 2 factorial, forming the experimental groups: DC-SL (control diet plus saline solution application); OL-SL (diet with soybean oil plus saline application); DC-rBST (control diet plus application of 320 mg rBST) and OL-rBST (diet with soybean oil plus the application of 320 mg rBST). The animals received two applications of 500 mg of …


Computed Tomography And Osteological Examination Of The Nuchal Perforations In Byzantine Cattle Skulls, Vedat Onar, Aleksander Chroszcz, Dominik Poradowski, Nazan Gezer İnce, Mehmet Ali̇ Polat, Rahmi̇ Asal Jan 2022

Computed Tomography And Osteological Examination Of The Nuchal Perforations In Byzantine Cattle Skulls, Vedat Onar, Aleksander Chroszcz, Dominik Poradowski, Nazan Gezer İnce, Mehmet Ali̇ Polat, Rahmi̇ Asal

Turkish Journal of Veterinary & Animal Sciences

This study presents computed tomography (CT) and osteological observations of nuchal perforations in the Middle-Late Byzantine cattle skulls, unearthed from Yenikapı Metro and Marmaray excavations (ancient Theodosius harbour), İstanbul, Türkiye. A total of 17 skulls with perforations of various size, ranging from elliptical to triangular, located in the nuchal (posterior) region of protuberantia intercornualis were examined. Rounded edges and smooth inner walls of these perforations indicated that they were formed when the animals were alive (ante mortem). The CT helped to understand the details of the caudal and dorsal part of head as well as the relationships between these perforations …


Genetic Variation At The Olr1, Anxa9, Myf5, Ltf, Igf1, Lgb, Csn3, Pit1, Mbl1,Cacna2d1, And Abcg2 Loci In Turkish Grey Steppe, Anatolian Black, And Eastanatolian Red Cattle, Özden Çobanoğlu, Sena Ardiçli Jan 2022

Genetic Variation At The Olr1, Anxa9, Myf5, Ltf, Igf1, Lgb, Csn3, Pit1, Mbl1,Cacna2d1, And Abcg2 Loci In Turkish Grey Steppe, Anatolian Black, And Eastanatolian Red Cattle, Özden Çobanoğlu, Sena Ardiçli

Turkish Journal of Veterinary & Animal Sciences

Native breeds are excellent sources of genetic variation. Anatolian native breeds are relatives of the first cattle domesticated and are ancestors of many European breeds. Therefore, this study aimed to evaluate the genetic variation of OLR1, ANXA9, MYF5, LTF, IGF1, LGB, CSN3, PIT1, MBL1, CACNA2D1, and ABCG2 markers in Turkish Grey Steppe, Anatolian Black, and East Anatolian Red cattle. The analysis included 367 cattle and the genotyping was performed by the PCR-RFLP. Population genetics indices including heterozygosity, the number of effective alleles, the polymorphism information content, fixation index, and the level of possible variability realization, and moreover, the genetic diversity …


Genetic Variation In The Bovine Myogenic Determination Factor 1 (G.782g>Apolymorphism) And Its Influence On Carcass Traits In Turkish Grey Steppe Cattle, Sena Ardiçli, Özden Çobanoğlu Jan 2021

Genetic Variation In The Bovine Myogenic Determination Factor 1 (G.782g>Apolymorphism) And Its Influence On Carcass Traits In Turkish Grey Steppe Cattle, Sena Ardiçli, Özden Çobanoğlu

Turkish Journal of Veterinary & Animal Sciences

MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic …


Novel Heat Index Models For Subtropical Region Based On Daily Milk Production Incrossbred Holstein Cows, Neeraj Kashyap, Atish Kumar Chakravarty, Bharti Deshmukh Jan 2021

Novel Heat Index Models For Subtropical Region Based On Daily Milk Production Incrossbred Holstein Cows, Neeraj Kashyap, Atish Kumar Chakravarty, Bharti Deshmukh

Turkish Journal of Veterinary & Animal Sciences

The quantification of the effect of heat stress imposed due to higher rate of heat gain on animal production is a prerequisite while estimating effects of heat stress. Temperature humidity index (THI) models developed empirically using meteorological parameters have been utilized as an indicator of heat stress. This study attempted to develop a heat index model with respect to productivity of the crossbred Holstein cows. More than 1.23 million daily milk yield records of 1860 crossbred cattle spread over a duration of 30 years were utilized. Statistically significant (p < 0.0001) partial regression coefficients were estimated as 0.0748 ± 0.0009 for dry bulb temperature (T$_{db}$) and -0.2228 ± 0.0011 for wet bulb temperature in model 1, -0.0182 ± 0.0005 T$_{db}$ and -0.1205 ± 0.0006 for vapour pressure in model 2, -0.0150 ± 0.0006 for Tdb and -0.1151 ± 0.0006 for dew point temperature in model 3 while as -0.1283 ± 0.0004 for T$_{db}$ and -0.0394 ± 0.0002 for relative humidity in model 4. The ratio of the partial regression coefficients of meteorological parameters (ß2/ß1) was -2.9791 in model 1, 6.8739 in model 2, 7.6731 in model 3 and 0.3073 in model 4. The developed THI models are based on the effect of meteorological parameters on daily productivity of the dairy animals, so the weightages to meteorological parameters were contrastingly different than the reported THI models. These models may successfully be used to evaluate the impact of heat stress on crossbred Holstein cows with respect to milk productivity and many other traits.


In Vitro Gas Production And Fatty Acids Biohydrogenation Of Diets Containing Different Unsaturated Fatty Acids Sources Plus Crude Glycerin, Arturo Samuel Gomez Insuasti, Yury Tatiana Granja-Salcedo, Julian Andres Castillo Vargas, Juliana Duarte Messana, Ana Paula De Oliveira Sader, Telma Teresinha Berchielli Jan 2021

In Vitro Gas Production And Fatty Acids Biohydrogenation Of Diets Containing Different Unsaturated Fatty Acids Sources Plus Crude Glycerin, Arturo Samuel Gomez Insuasti, Yury Tatiana Granja-Salcedo, Julian Andres Castillo Vargas, Juliana Duarte Messana, Ana Paula De Oliveira Sader, Telma Teresinha Berchielli

Turkish Journal of Veterinary & Animal Sciences

An in vitro trial was conducted to evaluate the effects of different unsaturated fatty acids (UFA) sources plus crude glycerin (CGL) on gas production and rumen biohydrogenation (RBH). Incubated diet corresponded to diets containing corn silage (30%) and concentrate (70%) composed of corn, urea, mineral salts, CGL, and different UFA sources as follows: no additional fat (NAF), rumen protected fat (RPF), soybean oil, linoleic acid (LA) or alpha-linolenic acid (LN). Methane concentrations in LA and LN were lower compared to NAF (p < 0.001), but when CH4 was expressed as a proportion of total gas, NAF and RPF showed the greatest values among evaluated UFA sources (p = 0.001). 18:1 t11 concentration showed a faster increase from 0 to 1 h and a slower decrease from 1 to 36 h of incubation, irrespective of UFA source. A higher C18:1 t11 production rate was observed from 1 h to 5 h of incubation, where LA and LN have higher values compared to NAF and RPF diets (p < 0.001). Diets with a high content of LA may be efficient as a nutritional approach to reduce methane production and RBH, resulting in positive effects on vaccenic acid concentrations.


Prevalence Of Gastrointestinal Parasitic Infections In Cattle And Sheep In Two Regions Ofromania, Catalin Bogdan Sirbu, Kalman Imre, Gheorghe Darabus, Tiana Suici, Bogdan Mates, Sorin Morariu Jan 2020

Prevalence Of Gastrointestinal Parasitic Infections In Cattle And Sheep In Two Regions Ofromania, Catalin Bogdan Sirbu, Kalman Imre, Gheorghe Darabus, Tiana Suici, Bogdan Mates, Sorin Morariu

Turkish Journal of Veterinary & Animal Sciences

Amongst ruminant livestock diseases, the gastrointestinal (GIT) helminths and protozoan parasite infections result in a significant socio-economic concern worldwide. During the period between October 2017 and November 2018, a total of 788 fresh fecal specimens from cattle (n = 303) and sheep (n = 485) were screened for the presence of gastrointestinal helminths and protozoan parasites in two historical regions (Crisana and Banat) of western Romania. Laboratory processing of fecal samples with the simple salt flotation (Willis) and sedimentation techniques, followed by microscopic analysis revealed that 86.1% of cattle and 92.6% of sheep were found to be positive for at …


Expression Profiles Of Lincrna And Mrna Related To Milk Yield And Milk Composition Traits In The Milk-Derived Exosomes Of Holstein And Doğu Anadolu Kırmızısı Cows, Selçuk Özdemi̇r, Ömer Eltas, Muhammed Hüdai̇ Çulha Jan 2020

Expression Profiles Of Lincrna And Mrna Related To Milk Yield And Milk Composition Traits In The Milk-Derived Exosomes Of Holstein And Doğu Anadolu Kırmızısı Cows, Selçuk Özdemi̇r, Ömer Eltas, Muhammed Hüdai̇ Çulha

Turkish Journal of Veterinary & Animal Sciences

This study aimed to demonstrate the expression profiles of lincRNAs and mRNAs affecting milk yield and composition traits in the milk-derived exosomes of Holstein and Doğu Anadolu Kırmızısı (DAK) cows. For this purpose, the locations of these specific lincRNAs and mRNAs were confirmed in quantitative trait loci. Then RT-PCR analysis was performed to identify the expression profiles of the lincRNAs and mRNAs. Lastly, correlation analysis was carried out between milk yield data from Holstein and DAK cows and expression levels of the lincRNAs and mRNAs. The findings showed that while lincRNAs and mRNAs associated with milk yield traits were upregulated …


Investigation Of Biotype, Genotype And Virulence Associated Genes Inpasteurella Multocida Capsular Type A Strains From The Respiratory Tract Of Cattle Andtheir Relationship With Disease Cases, Özgül Gülaydin, Kemal Gürtürk Jan 2020

Investigation Of Biotype, Genotype And Virulence Associated Genes Inpasteurella Multocida Capsular Type A Strains From The Respiratory Tract Of Cattle Andtheir Relationship With Disease Cases, Özgül Gülaydin, Kemal Gürtürk

Turkish Journal of Veterinary & Animal Sciences

Pasteurella (P.) multocida, which causes respiratory disease in cattle, can also be found commensally in the upper respiratory tract. Therefore, it is epidemiologically important to determine the possible difference between pathogenic and commensal strains for the diagnosis of the disease. In this study, phenotypic and genotypic characteristics and virulence associated genes of P. multocida capsular type A strains isolated from the respiratory tract of sick and healthy cattle were investigated. Also, the relationship between the disease status of animals and the characteristics of the isolates were evaluated. Total of 51 P. multocida capsular type A strains were biotyped and genotyped …


Optimized Oral Supplementation Of Vitamins Improves Feed Intake And Rumen Microbial Protein Synthesis In Deoni Cows, Ashwin Kandathil, Srinivas Bandla Jan 2019

Optimized Oral Supplementation Of Vitamins Improves Feed Intake And Rumen Microbial Protein Synthesis In Deoni Cows, Ashwin Kandathil, Srinivas Bandla

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to optimize oral doses of fat- and water-soluble vitamins in cattle using in vitro gas production model and to ascertain their effects on dry matter intake (DMI), nutrient digestibility, and rumen microbial protein (RMBP) synthesis. Doses optimized using the in vitro model were 3.66, 6.78, 16.3, 41.1, 3.87, 0.323, 4.12, and 0.055 mg/kg DMI, respectively, for thiamine, riboflavin, niacin, pantothenic acid, pyridoxine, biotin, folic acid, and B12 and 500 IU/kg DMI for vitamin K. Thirty-two adult, nonproducing Deoni cows (375 ± 13.4 kg body weight, BW) were randomly divided into four equal groups (n = …


Snake Envenomation In Two Cattle: Clinical/Laboratory Aspects And Treatment Using Equine-Derived Antivenin Of Viperidae, Nuri̇ Altuğ, Cafer Tayer İşler Jan 2019

Snake Envenomation In Two Cattle: Clinical/Laboratory Aspects And Treatment Using Equine-Derived Antivenin Of Viperidae, Nuri̇ Altuğ, Cafer Tayer İşler

Turkish Journal of Veterinary & Animal Sciences

In this case report, specific antivenin treatment for Viperidae snake envenomation in two cattle was defined for the first time. The diagnosis of snake envenomation was made according to the history and clinical findings. Clinical examination revealed systemic (tachycardia, dyspnea, cyanosis, hypersalivation, ruminal hypotony) and local (swelling of the leg and head) findings depending on the location of snake bites. Laboratory analysis determined granulocytic leukocytosis, monocytosis, decreased iron binding capacity, and increased CK and LDH enzyme activities. In the specific treatment of cases, equine-derived polyvalent antivenin containing antitoxic immunoglobulins against Viperidae species was administered. Additionally, 5% dextrose, antibiotics, dexamethasone, antihistamines, …


Electrocardiogram And Serum Cardiac Biomarkers Changes In Fmd In Cattle, Ali Abbas Nikvand, Seyedeh Missagh Jalali, Mohammad Nouri, Alireza Ghadrdan Mashhadi, Soroush Hassanpour Amirabadi Jan 2019

Electrocardiogram And Serum Cardiac Biomarkers Changes In Fmd In Cattle, Ali Abbas Nikvand, Seyedeh Missagh Jalali, Mohammad Nouri, Alireza Ghadrdan Mashhadi, Soroush Hassanpour Amirabadi

Turkish Journal of Veterinary & Animal Sciences

This study was designed to evaluate serum and electrocardiographic (ECG) changes in cattle with foot-and-mouth disease (FMD). Thirty-two cows with clinical signs of FMD and 13 healthy cows were randomly enrolled. After blood sampling, ECG was performed on 23 of the 32 ill cows. Serum circulatory troponin I (cTnI), CPK, LDH, and CK-MB activities were determined in the patient and control groups. Arrhythmias including ventricular tachycardia (VT), sinus tachycardia (ST), atrial fibrillation (AF), and premature ventricular complex (PVC) were seen in 4, 4, 2, and 1 of the patients, respectively. The patients had higher cTnI levels than the control group …


Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z Jan 2019

Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to examine resistance against cadmium (Cd$^{+2}$), copper (Cu$^{+2}$), lead (Pb$^{+2}$), mercury (Hg$^{+2}$), and manganese (Mn$^{+2}$) in Escherichia coli isolates recovered from cattle stool using phenotypic (agar dilution) and genotypic [polymerase chain reaction (PCR)] methods. In addition, the isolates were genotyped via enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Escherichia coli was isolated and identified from the 100 stool samples that were examined in the study. It was determined phenotypically that all isolates were sensitive to mercury; 97% of them showed resistance to cadmium; 69% showed resistance to copper; 24% showed resistance to lead; …


Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok Jan 2019

Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok

Turkish Journal of Veterinary & Animal Sciences

A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, …


Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova Jan 2019

Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the serological diagnostic potential of the Brucella recombinant outer membrane (rOMP25, rOMP31) and periplasmic proteins (rBP26, rSOD) in a comparative way using an indirect enzyme-linked immunosorbent assay (i-ELISA). Rabbit and/or mouse antibodies to Brucella whole cell and/or soluble protein preparations recognized all recombinant proteins used, which confirms the expression of target antigens in E. coli in active form. The recombinant proteins showed different antigenicity to antibodies of cattle kept on a brucellosis-affected (endemic) farm and/or a new focus of infection. Thus, the presence of anti-Brucella antibodies was confirmed by i-ELISA/rSOD in 79% …


Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar Jan 2019

Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar

Turkish Journal of Veterinary & Animal Sciences

A total of 20 domestic cattle (Bos taurus L.) and 15 water buffalo (Bubalis bubalis L.) skulls were analyzed in this study. All of the specimens belonged to female individuals. Using a total of 27 craniometric measurements from each of the skulls, 9 indices were calculated. Although there were statistically significant differences between the linear measurements of the skulls of both species, while calculating the indices with their ratios, the values of the measurements of the facial area were determinative in the craniology. Among these indices, the facial index 1 value was statistically significant (P < 0.01) in the comparison of these two species. On the other hand, while considering the orbit and foramen magnum measurements, in contrast with the transversal measurement, the height was more determinant for their index and form.


Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka Jan 2019

Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka

Turkish Journal of Veterinary & Animal Sciences

Anaplasmosis has become a major concern in the cattle industry throughout the world due to its great economic impact. The causative agents, Anaplasmaspecies, are primarily transmitted by ticks, occurring intracellularly within blood cells, with some species being zoonotic. In this study, the presence of Anaplasma spp. was investigated in the blood and milk of dairy cattle in the Philippines. Blood and milk samples were collected from 98 dairy cattle from selected farms in five provinces in the southern part of Luzon Island in the Philippines. After DNA extraction, a conventional PCR for the control gene actin was performed, followed by …


Detection And Characterization Of Extended-Spectrum Beta-Lactamase Genes(Bla$_{Tem}$ And Bla$_{Shv}$) Among Beta-Lactam-Resistant Fecal Coliforms Of Dairy Cattle From Chhattisgarh, India, Nidhi Rawat, Fateh Singh, Sadanand Devidas Hirpurkar, Chandrahas Sannat, Nitin Eknath Gade Jan 2018

Detection And Characterization Of Extended-Spectrum Beta-Lactamase Genes(Bla$_{Tem}$ And Bla$_{Shv}$) Among Beta-Lactam-Resistant Fecal Coliforms Of Dairy Cattle From Chhattisgarh, India, Nidhi Rawat, Fateh Singh, Sadanand Devidas Hirpurkar, Chandrahas Sannat, Nitin Eknath Gade

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to isolate the antimicrobial-resistant enterobacteria from cattle feces and to identify the extended-spectrum beta-lactamase (ESBL) and carbapenemase gene among them. A total of 70 cattle fecal swab samples were collected from Durg, Chhattisgarh, India, and cultured in nutrient broth. Bacterial isolation recovered 120 gram-negative bacteria. Antimicrobial susceptibility testing showed 90 (75%) coliform isolates (66 Escherichia coli, 20 Klebsiella sp., and two each of Enterobacter sp. and Citrobacter sp.) resistant to amoxicillin, amoxicillin-clavulanic acid, cefixime, ceftazidime, imipenem, or meropenem. Bacterial isolates showed the highest resistance rate for ceftazidime (61.6%), followed by cefixime and amoxicillin-clavulanic acid …


Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma Jan 2018

Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma

Turkish Journal of Veterinary & Animal Sciences

The present case report deals with the morphological (gross and histopathological) and histochemical characterization of Johne?s disease in a cow. The carcass was presented in a hidebound condition with sunken eyes and with a history of persistent and nonresponsive diarrhea. Necropsy revealed generalized gelatinization of the visceral and peripheral fat. Thorough gross examination unveiled major pathological changes in the digestive tract. The gallbladder was distended, with severely thickened walls and blood mixed with bile. The small intestine was severely congested and the mesenteric lymph nodes were enlarged. The opening of the small intestine showed marked thickening of the intestinal walls, …


Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska Jan 2018

Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska

Turkish Journal of Veterinary & Animal Sciences

The main aim of this study was to identify the association between the single nucleotide polymorphism (SNP) in the bovine interleukin-17A (IL-17A) gene and mastitis resistance. Additionally, the connection between the identified polymorphism and the somatic cell score (SCS) was tested. Two hundred and six Polish Holstein?Friesian cows were genotyped using the PCR-RFLP method. One SNP mutation (c.126G>A) was identified within the coding sequence of the analyzed gene. Results of logistic regression analysis displayed no significant association between the IL-17A genotype and clinical mastitis occurrence (Wald chi-square test = 0.74; P = 0.69). The GLM analysis demonstrated that genotype …


Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya Jan 2017

Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya

Turkish Journal of Veterinary & Animal Sciences

The purpose of the study was to use decision trees to predict increased levels of somatic cells in cow's milk. The material for the study comprised data collected in 2012-2014 from five farms in Poland equipped with an automatic milking system. Data on 803 Polish Holstein-Friesian cows were collected. In order to predict somatic cell count data mining techniques were used to build a graphical model of a decision tree. This study found that the most important factors to anticipate an elevated somatic cell count in cow's milk are milk conductivity, lactation stage, and lactation (primiparous and multiparous cow groups), …


Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni Jan 2017

Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni

Turkish Journal of Veterinary & Animal Sciences

Bovine parainfluenza virus type 3 (BPIV3) is a leading respiratory pathogen of cattle and is often implicated as a causative pathogen of bovine respiratory disease complex (BRDC). Three genotypes (BPIV3a-c) have been tentatively classified based on genetic and phylogenetic analyses. A BPIV3c isolate, designated NX49, was isolated using the Madin-Darby bovine kidney cell culture from cattle in western China, where feedlot cattle with severe respiratory disease symptoms were observed. Hemagglutination assay demonstrated that NX49 had low agglutination activity against guinea pig erythrocytes, whereas SD0835, the first BPIV3c strain reported in China, showed high agglutination activity. Phylogenic analysis indicated that NX49 …


Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut Jan 2017

Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3+ T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3+ T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. $\alpha$SMA positivity was mainly in the media of vessels. Smooth muscle …


A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi Jan 2017

A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi

Turkish Journal of Veterinary & Animal Sciences

Ticks are the main hematophagous arthropods and obligatory ectoparasites that are considered a vector of serious pathogens for animals and humans. The aims of the present survey were to assess the prevalence and diversity of ticks in cattle in Southeast Iran and to determine Coxiella burnetii in the ticks. From May 2014 to April 2015, 583 ticks were collected randomly from 257 cattle. Nested trans-PCR was performed to detect C. burnetii. The overall prevalence of hard tick infestation on cattle was 56.8%. In total, seven different species were identified: Rhipicephalus sanguineus sensu lato (32.6%), Hyalomma anatolicum (28.8%), Hyalomma excavatum (19%), …


Differences In Efficacy Between Gamithromycin, Tilmicosin, And Tulathromycin As Metaphylactic Treatments In High Risk Calves For Bovine Respiratory Disease, T. Miller, M. E. Hubbert, E. F. Schwandt, D. U. Thomson, C. D. Reinhardt Jan 2017

Differences In Efficacy Between Gamithromycin, Tilmicosin, And Tulathromycin As Metaphylactic Treatments In High Risk Calves For Bovine Respiratory Disease, T. Miller, M. E. Hubbert, E. F. Schwandt, D. U. Thomson, C. D. Reinhardt

Kansas Agricultural Experiment Station Research Reports

The cost of Bovine Respiratory Disease to the beef industry due to death, poorer conversions, and therapy is estimated to cost more than $3 billion per year. Identifying and mitigating Bovine Respiratory Disease in cattle can be difficult due to the increased susceptibility for Bovine Respiratory Disease in high risk cattle. One management option to minimize an outbreak of respiratory disease is the use of metaphylaxis, the mass treatment of a group of calves to reduce the incidence and adverse effects of respiratory disease on high risk animals. Criteria used to determine the necessity of metaphylactic treatment against Bovine Respiratory …


Comparison Of Four Ultrasonographic Approaches For The Diagnosis Ofacquired Reticular Diaphragmatic Hernia In Bovidae, Ashwani Kumar, Vandana Sangwan, Jitender Mohindroo, Narinder Singh Saini, Simrat Sagar Singh Jan 2017

Comparison Of Four Ultrasonographic Approaches For The Diagnosis Ofacquired Reticular Diaphragmatic Hernia In Bovidae, Ashwani Kumar, Vandana Sangwan, Jitender Mohindroo, Narinder Singh Saini, Simrat Sagar Singh

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to look at four ultrasonographic approaches that diagnose acquired reticular diaphragmatic hernia (RDH) using either left or right caudal thoracic approaches (4th/5th intercostal space) and compare them to those made more cranially (1st/2nd intercostal space). Each sonographic observation was made in 27 unsedated, standing animals (22 buffaloes and 5 cattle) that were surgically confirmed for RDH. Using the right caudal thoracic approach, the herniated reticulum was seen as medial to the thoracic wall at a depth of 3-5 cm and adjacent to the cardiac silhouette. With the left caudal thoracic approach, the herniated reticulum …


Twenty-Four Hour Holter Monitoring In Finishing Cattle Housed Outdoors, D. A. Frese, J. D. Thomason, C. D. Reinhardt, S. J. Bartle, D. N. Rethorst, G. H. Loneragan, E. F. Schwandt, D. U. Thomson Jan 2017

Twenty-Four Hour Holter Monitoring In Finishing Cattle Housed Outdoors, D. A. Frese, J. D. Thomason, C. D. Reinhardt, S. J. Bartle, D. N. Rethorst, G. H. Loneragan, E. F. Schwandt, D. U. Thomson

Kansas Agricultural Experiment Station Research Reports

Ambulatory electrocardiogram monitoring, in the form of Holter monitoring, has been used in human and veterinary medicine for decades as an aid in the diagnosis and determination of appropriate therapy of heart rhythm disturbances. Within veterinary medicine, Holter monitors have been primarily used in companion animal species, yet little attention has been given to food animal species. Moreover, the heart rhythm in clinically normal cattle fed high concentrate diets and housed outdoors in confined drylot facilities has not been previously reported. In order to properly identify pathologic arrhythmias in cattle, the normal rhythm and arrhythmia prevalence in healthy cattle should …


Molecular Identification Of Bovine Papillomaviruses In Dairy And Beef Cattle: First Description Of Xi- And Epsilonpapillomavirus In Turkey, Veysel Soydal Ataseven, Özgür Kanat, Yaşar Ergün Jan 2016

Molecular Identification Of Bovine Papillomaviruses In Dairy And Beef Cattle: First Description Of Xi- And Epsilonpapillomavirus In Turkey, Veysel Soydal Ataseven, Özgür Kanat, Yaşar Ergün

Turkish Journal of Veterinary & Animal Sciences

In the current study, 23 papilloma/tumor-like samples obtained from cattle having clinical lesions and 9 blood samples collected from healthy-appearing cattle in Turkey were examined for bovine papillomavirus (BPV) DNA using the degenerate primers FAP59/64, and the different types of BPV were distinguished by type-specific primer sets. Furthermore, histopathological studies of papillomavirus were performed. A total of 7 BPV types (BPVs 1, 2, 3, 4, 6, 8, 9), including genera Deltapapillomavirus, Xipapillomavirus, and Epsilonpapillomavirus, were identified. In all samples, BPV-1 was the most common genotype (90.6%). Overall, coinfections were determined in 26 of the examined samples (81.3%), and coinfection with …


Cla And Epa Inhibit Lps-Induced Prostaglandin Release From Bovine Endometrial Cells Through An Nf-?B-Dependent Signaling Mechanism, Lokenga Badinga, Mehmet Şükrü Gülay, Alan Ealy Jan 2016

Cla And Epa Inhibit Lps-Induced Prostaglandin Release From Bovine Endometrial Cells Through An Nf-?B-Dependent Signaling Mechanism, Lokenga Badinga, Mehmet Şükrü Gülay, Alan Ealy

Turkish Journal of Veterinary & Animal Sciences

Although their action in antagonizing arachidonic acid metabolism is a key antiinflammatory effect of conjugated linoleic acid (CLA) and omega-3 polyunsaturated fatty acids (n-3 PUFA), these molecules have other antiinflammatory effects that might occur upstream of altered eicosanoid production. We examined the effects of two CLA isomers (c9,t11 and t10,c12 isomers), n-3 PUFA (EPA and DHA), and ΚNF-ΚB inhibitor (ammonium pyrrolidine dithiocarbamate (PDTC)) on lipopolysaccharide (LPS)-induced secretion of prostaglandins (PGE2 and PGF2a) by bovine endometrial (BEND) cells. LPS increased PGE2 and PGF2a concentrations in BEND cell-conditioned media in a concentration- and time-dependent manner. The c9,t11 CLA isomer and EPA decreased …