Research Methods in Life Sciences Commons^™

Respiratory Chain Activity And Protein Expression In Skeletal Muscles From High And Low Feed Efficient Swine Within The Same Genetic Line, Earsie L. Alcorn De Feliz

Graduate Theses and Dissertations

Current as well as past studies have provided evidence for a connection between macroeconomic/agricultural and cellular/biochemical basis of feed efficiency (FE; gain:feed) in farm animal production. Our investigation of respiratory chain activity, mitochondrial protein oxidation and protein expression was an expansion to the subcellular/molecular level. In this study we investigated the entire complex (Cx) of the electron transport chain (ETC) for differences in Cx activities in mitochondria isolated from skeletal muscle in high (HFE) and low (LFE) feed efficient swine within the same genetic line. Review of literature provided information for the connection between feed efficiency, mitochondrial function, and muscle …

Mitochondrial Contributions To Disuse Atrophy: Let’S Talk About Sex, Megan Rosa-Caldwell

Graduate Theses and Dissertations

Skeletal muscle atrophy is a hallmark of many pathologies and is associated with disease prognosis, quality of life, and mortality. Specifically, lack of contractile activity of the muscle can result in disuse-associated atrophy. Disuse-induced atrophy is a common phenomenon in intensive care unit (ICU) patients and significantly impacts patient prognosis and ability to transition of out of intensive care. However, effective therapeutics to mitigate disuse-associated atrophy do not exist, clearly demonstrating the scientific and clinical need to further understand mechanisms contributing to disuse atrophy. In particular, a significant portion of the scientific literature has utilized male models, yet there is …

Effects Of Supplementing Crystalline L-Valine And L-Isoleucine And A Novel Threonine Biomass In Reduced Crude Protein Diets Fed To Broilers, Derrell Trevor Lee

Graduate Theses and Dissertations

Poultry nutritionists continue to identify methods to lower feed price without compromising broiler growth performance, processing yields, or animal well-being. A common approach is to reduce dietary crude protein (CP), the second most expensive component in a broiler diet. Subsequently, reducing CP will reduce the inclusion levels of intact protein sources (e.g., soybean meal [SBM]), which can be accomplished by supplementing individual amino acids (AA). This methodology not only reduces diet cost, pending if feed-grade AA are more economical, but can contribute to improving broiler health and environmental sustainability. Therefore, two experiments were conducted to evaluate individual feed-grade AA in …

Distillers Dried Grains With Solubles: Determination Of Metabolizable Energy Values Using Regression-Based Assays And Their Correlation With Chemical Composition, Kenia Mitre

Graduate Theses and Dissertations

The inherent variability and lack of standardization of in vivo assays to determine the metabolizable energy (ME) of DDGS has led to inconsistent values and limited its potential to use in broiler diets. Therefore, two experiments were conducted to characterize the content of nitrogen-corrected ME (MEn) in 11 DDGS sources obtained from various ethanol plants using a regression-based broiler assay and to attempt to develop equations to predict MEn based on its chemical composition. In experiment 1, the objectives were to determine the influence of inclusion level and feed intake on the resulting ME and MEn values of a single …

Nutritional Strategies For Feeding Broiler Breeders On Hen And Progeny Performance, Antonio Beitia

Graduate Theses and Dissertations

Over the last 60 plus years, broiler breeders have been under an incredible amount of genetic pressure. This genetic pressure comes from the continually increasing consumer demand for poultry products. The objective of this dissertation is to understand the effects of different nutritional and management practices on broiler breeder’s performance. The first series of experiments investigate the impact of broilerization on hen and early progeny performance. Broilerization is a process only used by genetic companies. The results from these experiments showed the adverse effects of broilerization on chick quality and hen performance. Follow up trials were performed to determine the …

Effect Of The Prospect Of Transport On Captive Tiger Behavior And Fecal Cortisol In Naïve And Experienced Tigers, Callan Lichtenwalter

Graduate Theses and Dissertations

With close to 5,000 captive tigers in the United States, it is vital to ensure that these tigers are receiving adequate welfare whether they are in an accredited facility, or privately owned. The goal of this study was to assess whether captive tigers at a rescue facility with experience being transported outside of the facility would respond differently to the presentation of their transport vehicle than their naïve counterparts who had only been transported within the facility. The behavior of 5 naïve and 7 experienced tigers (n = 12) located at Turpentine Creek Wildlife Refuge in Eureka Springs, Arkansas was …

The Effects Of Excess Dietary Sulfur Using Supplemental Sodium Sulfate On Beef Steer, Heifer, And Progeny Productive And Physiological Responses, Jamie Hawley

Graduate Theses and Dissertations

For studies 1 through 3, 20 steers of predominantly Angus breeding, were stratified by body weight (279 ± 13.2 kg), assigned randomly to 6 paddocks, and fed a low S ground corn and soybean meal supplement that did not contain any byproduct feeds (0.31% total dietary S; LS) or LS supplement with an additional 0.25% S provided from sodium sulfate (Na2SO4; 0.58% total dietary S; HS) for a 114-d growing phase. Steers were moved to feedlot (373 ± 0.2 kg), remained on prior dietary S treatments, and fed corn and soybean meal diets (0.19 and 0.42% total dietary S; LS …

Quantifying Greenhouse Gas Emissions And Ammonia Volatilization From Soil Amended With Excreta From Ruminants Fed Alternative Forage-Based Diets, Samuel Park

Graduate Theses and Dissertations

Forage-based ruminant diets supplemented with condensed tannins or polyphenolic-containing legumes may alter nitrogen (N) metabolism in the animal and reduce gas emissions from soil receiving excreta. The objective of the study was to determine if soil receiving urine or manure from sheep fed forage diets supplemented with condensed tannin or polyphenolic-containing legumes would decrease N gas emissions. Two field trials were conducted: in the first trial, sheep were fed alfalfa (Medicago sativa L.) silage diets supplemented with 0, 9, 18, or 27% sericea lespedeza (Lespedeza cuneate [Dumont de Courset] G. Don) (n = 4). In the second trial sheep were …

Development Of Arkansas Net Energy Equation, Katie Hilton

Graduate Theses and Dissertations

The modern broiler is growing at a rapid rate generating tremendous amounts of heat. A sensitive Net Energy (NE) system is needed to measure body heat production (HP) generated primarily by daily maintenance and synthesis and degradation of myofibrillar and sarcoplasmic protein. The first two chapters present evaluation of the classic way to calculate NE versus a new methodology; the Arkansas NE (Ark NE) system, with birds from two genetic lines fed diets with different AA content or different ME content in two different environmental temperatures.

Utilizing together the Net Energy maintenance (NEm), determined from indirect calorimetry, and Net Energy …

Lesioning Of The Nucleus Of The Hippocampal Commissure Followed By Food Deprivation Stress In Birds Demonstrates Simultaneous Involvement In Both The Hypothalamo-Pituitary-Adrenal Axis And The Hypothalamo-Pituitary-Thyroid Axis, Michael Thomas Kidd Jr.

Graduate Theses and Dissertations

The hypothalamo-pituitary-adrenal (HPA) axis is the regulatory system for the neuroendocrine stress response within vertebrates. Within the HPA axis corticotropin releasing hormone (CRH) is a major regulator and driving hormone. A structure named the nucleus of the hippocampal commissure (NHpC) has been found to contain CRH neurons and also these neurons respond to early food deprivation stress significantly prior to the paraventricular nucleus (PVN), the major driving nucleus of the classic neuroendocrine HPA axis. The objective of this study was to perform a knock down of the NHpC via electrolytic lesioning, thus eliminating a significant portion of its population of …

Evaluation Of Microbiome On Chicken Necrotic Enteritis And Growth Performance, Mussie Abraha

Graduate Theses and Dissertations

Necrotic enteritis (NE) has re-emerged following restriction of antimicrobial usage and costs $6 billion every year worldwide. The primary objective of the studies was to evaluate prevention and treatment of NE using a microbiota metabolic product, secondary bile acid deoxycholic acid (DCA), in drinking water. Day-old birds were tagged and placed in floor pens. In experiments 1 and 2, the birds were infected with Eimeria maxima (Em) at d 18 and C. perfringens at d 23 and 24 and the birds were euthanized at d 26. In experiment 3, birds were infected with Eimeria at d 20 and C. perfringens …

Detection Of Woody Breast Condition In Broiler Carcasses And Product Utilization In Further Processing, Juan Pablo Caldas Cueva

Graduate Theses and Dissertations

Woody Breast (WB) myopathy causes significant economic losses to the global poultry industry and the lack of objective, non-invasive and rapid methods for its detection as well as profitable alternatives for its utilization are contributing factors. This dissertation is divided into two main areas. The first area aimed to determine if there are carcass conformation changes that can be used to identify WB characteristics in commercial broilers using image analysis. In the first experiment, images of broiler carcasses were captured before evisceration to obtain and evaluate carcass measurements from the breast region. Eight image measurements and four ratios were used …

Evaluation Of Anthelmintic Therapies In A Fall Calving Beef Cowherd, Laine Zammit

Graduate Theses and Dissertations

Internal parasitism inevitability prompts economic loss in beef cattle production by decreasing growth performance and reproductive traits. Today, the most widely used class of anthelmintic used to treat parasitism, is the macrocyclic lactone. Many studies have conflicting results on the efficacy of macrocyclic lactones (ML) efficacy against internal parasitism. The objective of this study was to evaluate the effectiveness of moxidectin and eprinomectin, two of the MLs, on cow performance. Multiparous fall calving, crossbred beef cows (n = 106) were allocated randomly to 1 of 3 anthelmintic treatments: 1) Negative control (CON), in which cows did not receive an anthelmintic, …

Associations Among Beef Cattle Genotypes, Neospora Caninum Infection, And Reproductive Performance, Ryan James Page

Graduate Theses and Dissertations

Reproductive performance is crucial for sustained financial success in the beef cattle industry. This dissertation includes a population study that quantified the incidence of Neospora caninum infections in the central region of the United States and tested its relationship with reproductive performance in beef cattle. Trial one of that study concluded that 6.9% of open, replacement heifers (n = 1306) tested seropositive. The second trial in that project found that 9.6% of the breeding age females (n = 500) tested were seropositive for Neospora caninum; and that state in which the cattle lived and age impacted (P < 0.05) infection rate. Breed composition, number of farm dogs on the ranch, and use of total mixed rations were not associated (P > 0.1) with seropositive …

Effects Of Lasalocid And Energy Supplementation On Forage Intake, Energy Metabolism, And Performance Of Cattle Grazing Wheat Pasture, Autumn Tayler Pickett

Graduate Theses and Dissertations

Cattle grazing wheat pasture have the potential to gain BW exceptionally well, but excessive nitrogen intake results in increased excretion and increased greenhouse gas (GHG) emissions. Supplemental concentrates with the addition of an ionophore given to ruminants grazing wheat is a potential practice for producers to increase nitrogen efficiency while decreasing GHG emissions. Therefore, the objective of this experiment was to quantify the effects of energy (2.95 kg/d) and lasalocid (200 mg/hd/d) supplementation on nutrient intake, energy metabolism, respiratory gas fluxes, and performance of grazing cattle. Methane emissions were not affected (P = 0.58) by treatment, hence methane intensity (P …

Establishing An Ideal Inclusion Rate Of Fermented Soybean Meal And Sodium Butyrate On Growth Performance, Complete Blood Cell Count, And Nutrient Utilization In Nursery Pigs, Kristopher A. Bottoms

Graduate Theses and Dissertations

To evaluate increasing levels of sodium butyrate (SB) in nursery diets on growth performance (Experiment 1 & 2), complete blood cell count (Experiment 2), and the optimal level of fermented soybean meal for maximum performance in weanling pigs (Experiment 3), weaned pigs were blocked with initial body weight (BW) and allotted to dietary treatments. Treatments were: 1) Control (C) moderately complex corn-soybean-meal based supplemented with 0.05% benzoic acid (BA), but devoid of SB; C diet supplemented with 0.05%, 0.10%, or 0.15% SB (Experiment 1). Treatments in experiment 2 consisted of 1) a moderately complex corn-soybean-meal based diet devoid of SB …

Estimation Of Genetic Components Related To Infectious Bovine Keratoconjunctivitis Susceptibility In Angus And Angus Derived Cattle Produced In The Southern United States, Eric Oxford

Graduate Theses and Dissertations

The economic impact of infectious bovine keratoconjunctivitis (IBK) has been documented in many parts of the world. Many researchers have observed that prevention of this disease is very difficult given current methodologies. This is primarily due to the multifactorial nature of this disease. The objective of this dissertation was to determine the impact of IBK on calf performance and estimate genetic parameters, heritability and estimated breeding values for IBK susceptibility. Data were analyzed using PROC GLIMMIX of SAS; while genetic parameters were estimated using a linear animal model for both single- and two-traits through MTDFREML. Additional evaluations calculated heritability using …

Characterization Of The Swine Gut Microbiome Dynamics, Culturomics And Probiotics, Xiaofan Wang

Graduate Theses and Dissertations

The gastrointestinal tract is a critical place where the immune system concentrates many of its protective functions. It exerts both physical and biochemical defenses against invading foodborne pathogens and other hazards. In pigs, the gut microbiota community assembled postnatal plays a very important role in the development of multiple physiologic aspects, such as development of the gastrointestinal tract, nutrient exchange, immune defense against foreign pathogens and growth performance. Microbiome-host communication changes with age. Despite recent advances in understanding the swine gut microbiome at different growth stages, a comprehensive longitudinal study of the lifelong dynamics of the swine gut microbiome is …

The Impacts Of Administering Metabolites Of Saccharomyces Cerevisiae On Broiler Performance, Yields And Salmonella Content Of Component Portions, Bill Douglas Potter

Graduate Theses and Dissertations

The impacts of using health-promoting Saccharomyces cerevisiae fermentation metabolites in poultry production and processing can be measured in respect to multiple measures of success. Traditionally this yeast-based compound has been administered to poultry, livestock, poultry, and other species to improve animal performance and production volume output. In addition, Saccharomyces cerevisiae fermentation metabolites have also been shown in more recent research to reduce colonization of pathogenic bacteria in the host organism’s gastro-intestinal tract. In this dissertation, the impacts of administering a functional ingredient containing Saccharomyces cerevisiae metabolites on broiler performance measures and pathogen reduction were measured. One of the studies in …

Study Of Three Potential Variables That May Impact The Maximum Shear Force And Shear Variation During The Growth And Transport Of Mixed Sex Broilers Grown In Commercial Poultry Houses, Mark Christie

Graduate Theses and Dissertations

Temperature conditions during the transportation to the processing facility of commercial broilers have a direct impact on the meat texture, specifically maximum shear force and shear variation of cooked breast meat. Analysis of a calendar year of flock data (N=346), reveals that the largest impact of transport conditions, specifically temperature, is on the standard deviation of the flocks range of cooked shear values. The optimal transport temperature that yields broiler breast meat with average flock minimum shear and standard deviation values is approximately 60°F. The shear values for flocks at or near 60°F are on average 1 kg less than …

Optimizing Methods To Determine Metabolizable Energy Values Of Feed Ingredients For Broilers, Skyler P. West

Graduate Theses and Dissertations

Determination of metabolizable energy (ME) and amino acid (AA) digestibility values of single feed indigents continues to be two of the most important aspects for successful least-cost poultry feed formulation. It would be advantageous if a common diet type could be utilized to determine both ME and AA digestibility values of feed ingredients within a single assay. Two experiments were conducted to evaluate the effect of basal diet type and excreta collection method on the ME value of single feed ingredients determined in broiler chicks using the regression method. In experiment 1, the objective was to investigate the influence of …

Efficacy Of Natural Compounds With Novel Carrier Systems For Controlling Campylobacter Jejuni In Post-Harvest Poultry, Sandip Shrestha

Graduate Theses and Dissertations

Campylobacter jejuni, a leading cause of bacterial gastroenteritis worldwide, is strongly associated with the consumption and/or mishandling of raw contaminated poultry products. Thus, interventions aiming to reduce C. jejuni counts on poultry products could greatly reduce the incidence of human campylobacteriosis. In the first study, the efficacy of a generally recongnized as safe (GRAS) compound, carvacrol (CR; derived from oregano oil), as an antimicrobial wash treatment to reduce C. jejuni on chicken skin was evaluated. Three delivery systems of CR: suspension, emulsion and nanoemulsion were used. C. jejuni counts were reduced up to 4 log10 cfu/sample by 2% dose of …

The Use Of Probiotics To Control The Microbial Load Present In Commercial Broiler Chicken Hatch Cabinets As An Alternative To Formaldehyde Fumigation, Lucas Elzie Graham

Graduate Theses and Dissertations

Formaldehyde has been used for decades as a disinfectant in the poultry hatchery. Hatch cabinets are treated with formaldehyde to control the microbial bloom that occurs inside of the hatch cabinet as the hatch progresses. Even with formaldehyde being a known human carcinogen and the detriment that it has on living creatures millions of chicks are exposed to formaldehyde in the hatch cabinet. In these experiments we tested a lyophilized probiotic spray inside of the hatch cabinets from day nineteen to day twenty one to control the microbial bloom that occurs. Hatch cabinet environments were sampled in six experiments. Media …

In Ovo Evaluation Of Floramax-B11 On Marek's Disease Hvt Vaccine Protective Efficacy, Hatchability, Microbiota Composition, Morphometric Analysis, And Salmonella Enteritidis Infection In Broiler Chickens, Kyle Dean Teague

Graduate Theses and Dissertations

It has previously been shown that effective probiotics can accelerate gut maturation and the development of a normal microflora in poultry. This results in increased resistance to enteric pathogens encountered by chicks early in life. Our objective in experiments 1 and 2 was to evaluate the effect of in ovo administration of FloraMax®-B11 (FM) on Marek’s disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy. In Exp. 1, day 18 White Leghorn embryos were randomly distributed in four groups, 1) HVT vaccinated, no MDV challenge, 2) HVT + FM vaccinated, no MDV challenge, 3) HVT vaccinated, challenge with virulent MDV, …

Genetic Polymorphisms Of The Glucocorticoid Receptor And Interleulin-8 Receptor Genes And Their Relationship To Production Traits And Hair Coat Scores In Crossbred Cattle, Avery B. Deaton, Laura Meyer, Jeremy Powell, Charles F. Rosenkrans Jr.

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Little is understood about how the diversity of genes, specifically the glucocorticoid receptor (GR) and interleukin-8 receptor (CXCR2), are related to reproductive health and how this affects physical traits in cattle. Glucocorticoid receptors have been positively associated with higher milk yields, lactose content, feed intake, and feed conversion rates. Interleukin-8 genes are part of the innate immune response and help with many aspects of female reproductive health, such as protecting the embryo from the maternal immune system during pregnancy. The objective of this research was to identify polymorphisms in the GR and CXCR2 genes and to associate genotypes between the …

Arkansas Animal Science Department Report 2016, Paul Beck, Jason Apple, Shane Gadberry, Beth Kegley, Charles Rosenkrans Jr.

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Arkansas Animal Science Department Report 2015, Paul Beck, Jason Apple, Beth Kegley, Charles Rosenkrans Jr.

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Arkansas Animal Science Department Report 2014, David L. Kreider, Paul Beck

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Arkansas Animal Science Department Report 2013, David L. Kreider, Paul Beck

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Arkansas Animal Science Department Report 2012, David L. Kreider

Arkansas Agricultural Experiment Station Research Series

No abstract provided.