Research Methods in Life Sciences Commons^™

Snp-Based Breeding For Broiler Resistance To Ascites And Evaluation Of Correlated Production Traits, Katie Pepper Lee, Nicholas B. Anthony, Sara K. Orlowski, Douglas D. Rhoads

Biological Sciences Faculty Publications and Presentations

Background

The goal of this study was to evaluate marker-assisted selection (MAS) in broiler chickens using previously mapped gene regions associated with ascites syndrome incidence. The second-generation MAS products were assessed for impact on ascites phenotype and whether there were associated changes in important production traits. Previously, we used whole genome resequencing (WGR) to fine-map 28 chromosomal regions as associated with ascites phenotype in our experimental ascites broiler line (Relaxed, REL) based on a hypobaric chamber challenge. Genotypes for single nucleotide polymorphisms (SNPs) in mapped regions on chromosomes 2 and 22, were used for MAS in our REL line. After …

Bovine Respiratory Microbiota Of Feedlot Cattle And Its Association With Disease, Jianmin Chai, Sarah F. Capik, Beth Kegley, John T. Richeson, Jeremy G. Powell, Jiangchao Zhao

Animal Science Faculty Publications and Presentations

Bovine respiratory disease (BRD), as one of the most common and costly diseases in the beef cattle industry, has significant adverse impacts on global food security and the economic stability of the industry. The bovine respiratory microbiome is strongly associated with health and disease and may provide insights for alternative therapy when treating BRD. The niche-specific microbiome communities that colonize the inter-surface of the upper and the lower respiratory tract consist of a dynamic and complex ecological system. The correlation between the disequilibrium in the respiratory ecosystem and BRD has become a hot research topic. Hence, we summarize the pathogenesis …

Studies Of Avian Nasal Mites (Acari: Rhinonyssidae And Ereynetidae) And Their Interaction With The Brood Parasite Brown-Headed Cowbird (Molothrus Ater: Icteridae) And Phylogenetic Inferences Of The Genus Ptilonyssus (Rhinonyssidae) On Different Passerine Hosts Associated To Three States In The Us, Alexis D. Hilario Perez

Graduate Theses and Dissertations

Nasal mites are endoparasites that spend their entire life cycle inside the nasal cavities and respiratory passages of birds. The Brown-Headed cowbird (Molothrus ater) (BHCO) is an icterid bird that uses brood parasitism as a reproductive strategy in which it lays an egg in the nest of a different bird species and allows the host to raise its young. Interestingly, nasal mites reported from cowbirds represent the same species found infesting other icterids and other common host groups. In the first study, I examined how diversity and host prevalence might change in a large sample size of BHCO associated with …

Pets And Problems: Exploring Community Patterns In Calls For Animal Services, Paige Dejarnett

Sociology and Criminology Undergraduate Honors Theses

Pets have historically been viewed as family members, children, property, or economic resources. However, research surrounding animal maltreatment has expressed this issue as an individually-based problem, rather than a community-based phenomenon. Correlations have been found between animal cruelty, antisocial behaviors, and future interpersonal violence, whether this correlation be a predictive relationship, or a resultant relationship. Past research has also found correlations between animal treatment practices and the rural/urban differences of this behavior. However, there are many community-based indicators that have not been explored to understand the distribution of animal maltreatment. This study aims to explore these ideas by analyzing the …

Investigation Of Histomoniasis Prevention In Poultry, Lesleigh Beer

Graduate Theses and Dissertations

Histomonas meleagridis is the etiological agent of histomoniasis, also commonly known as blackhead disease. This protozoal disease of poultry is detrimental to turkeys with flock mortalities often reaching 80-100%, although other gallinaceous birds are susceptible. Since the voluntary removal of nitarsone in 2015, the poultry industry is suffering with no approved prophylactics, therapeutics, or vaccines for this disease. The objectives of this dissertation were to evaluate multiple methods for prevention or control of histomoniasis, including dietary chemoprophylaxis and vaccination. Specifically, this research evaluated quinine as a chemoprophylactic candidate (Chapter 3) or live-attenuated H. meleagridis as vaccine candidates (Chapter 4) in …

Canine Microwear In Relation To Diet In Sumatran Primates And African Great Apes, Putu Pujiantari

Graduate Theses and Dissertations

This thesis considers canine microwear in relation to diet in five Sumatran primates (Pongo abelii, Hylobates lar, Hylobates agilis, Presbytis thomasi, and Macaca fascicularis) and two African great apes (Pan paniscus and Pan troglodytes schweinfurthii) using both microwear texture analysis and microwear feature analysis techniques. Statistical results for texture analysis show that there are significant differences in scale of maximum and heterogeneity. This indicates that some species have large pits on their canine surfaces, having these dominated by deep features at coarse scale yet have a slight microwear heterogeneity. For feature analysis, all variables show statistically significant variation. Variance in …

Reducing Dietary Crude Protein: Characterization Of Branched-Chain Amino Acid Antagonism In Modern Broiler Chickens, Craig William Maynard

Graduate Theses and Dissertations

Reducing the crude protein content of broiler diets while maintaining performance has been a primary research area in nutrition. During the late 20th century, the bulk of this research has involved supplementing broiler diets with feed-grade amino acids and proving the economic benefits of their additions. To date, widespread adoption of feed-grade amino acids is limited to methionine, lysine, threonine, and to some extent valine. With valine and isoleucine gaining prevalence in commercial formulation, classic data on the antagonisms associated with the branched-chain amino acids has returned to the forefront of practical broiler nutrition research. Therefore, a series of 23 …

Local And Systemic Cytokine, Chemokine, And Fgf Profile In Bacterial Chondronecrosis With Osteomyelitis (Bco)-Affected Broilers, Alison Ramser, Elizabeth Greene, Robert Wideman, Sami Dridi

Poultry Science Faculty Publications and Presentations

Complex disease states, like bacterial chondronecrosis with osteomyelitis (BCO), not only result in physiological symptoms, such as lameness, but also a complex systemic reaction involving immune and growth factor responses. For the modern broiler (meat-type) chickens, BCO is an animal welfare, production, and economic concern involving bacterial infection, inflammation, and bone attrition with a poorly defined etiology. It is, therefore, critical to define the key inflammatory and bone-related factors involved in BCO. In this study, the local bone and systemic blood profile of inflammatory modulators, cytokines, and chemokines was elucidated along with inflammasome and key FGF genes. BCO-affected bone showed …

Blood–Brain Barrier Breakdown And Astrocyte Reactivity Evident In The Absence Of Behavioral Changes After Repeated Traumatic Brain Injury, Celeste Dunn, Nasya Sturdivant, Sara Venier, Syed Ali, Jeffery Wolchok, Kartik Balachandran

Biomedical Engineering Faculty Publications and Presentations

Repeated traumatic brain injuries (TBIs) cause debilitating effects. Without understanding the acute effects of repeated TBIs, treatment options to halt further degeneration and damage cannot be developed. This study sought to examine the acute effects of blood–brain barrier (BBB) dysfunction, edema, inflammation and behavioral changes after either a single or double TBI using a C57BL/6 mouse model. We examined the effects of one or two TBIs, of either a mild or moderate severity. Double injuries were spaced 7 days apart, and all analysis was performed 24 h post-injury. To examine edema and inflammation, protein levels of glial fibrillary acidic protein …

Directed Genome Evolution To Identify Genes For Macrophage Survival By Staphylococcus Agnetis, Sonali Lakshika Anne Lenaduwe Lokuge

Graduate Theses and Dissertations

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a debilitating infection that negatively impacts animal welfare and costs the broiler industry billions of dollars annually. We have previously isolated Staphylococcus agnetis 908 from BCO samples obtained from broilers at the University of Arkansas research farm. This isolate can induce BCO lameness at greater than 50% in broilers exposed to the pathogen in drinking water. We found that S. agnetis 908 is capable of surviving and escaping macrophages compared to a closely related cattle isolate,1379. Through Directed Genome Evolution (DGE) we identified that this difference is at least partially associated with an alanine …

Host-Parasite Interactions Within Food Webs, Adam Zvanut Hasik

Graduate Theses and Dissertations

Parasitism is one of the most common life history strategies employed in nature, yet the effects of parasites are often thought to be minimal, and the vast majority of studies fail to consider parasites and their effects on host organisms. This is likely a problem, as the magnitude of parasite-mediated effects on their hosts can be quite large. Additionally, the effects of parasites are known to extend beyond the host to affect other species interactions. I used a series of approaches to gain a more integral understanding of host-parasite interactions by studying (1) the effects of parasites on biotic interactions …

Microfabrication And Electrochemical Characterization Of A Novel Su-8 Probe With An Array Of Individually Addressable Electrodes Suitable For Redox Cycling Experiments In Ultra-Small Volumes, Mahsa Lotfi Marchoubeh

Graduate Theses and Dissertations

Redox cycling is an electrochemical technique that utilizes closely spaced generator and collector electrodes to cycle reversible redox species between their oxidative states. With advantages in signal amplification, selectivity of species based on their electrochemical reaction mechanism, and limited or no background subtraction, this technique is well suited for selective detection of important electrochemically active molecules such as dopamine at basal or slowly changing levels.

Miniaturized medical devices have become an area of great interest for measurement of chemicals in limited volumes with low concentrations or in sensitive tissues. A probe on a polymeric SU-8 substrate with suitable dimensions and …

Evaluation Of Weight Gain, Feed Intake, Feed Conversion And Oocyst Shedding Of Eimeria Maxima And Eimeria Acervulina In Broiler Chickens, Lauren Laverty

Poultry Science Undergraduate Honors Theses

The purpose of the present study was to evaluate the day and the time of sample collection of an

experimental challenge with Eimeria maxima (EM) and Eimeria acervulina (EA) in broiler chickens. One day old male Cobb-Vantress broiler chickens were randomly allocated to one of three groups with ten replicates (n=8 chickens/replicate). Chickens were placed in battery cages with a controlled age-appropriate environment: Group 1) Negative control (no challenge or treatment); 2) Challenge control (Eimeria challenge only); 3) Challenge + Salinomycin. Challenged chickens were orally gavaged with the mixed culture of EM/EA (10,000 sporulated EM containing 4% wild-type EA) …

Survey Of Ticks And Tick-Borne Pathogens Associated With Feral Swine (Sus Scrofa) In Arkansas, Haylee Campbell

Graduate Theses and Dissertations

Feral hogs (Sus scrofa L.) are an invasive species throughout the southeast United States and found in every Arkansas county. As feral hogs invade new habitat, they can disrupt ecosystems, damage agriculture systems, and bring ticks and tick-borne pathogens with them. There are no surveys of the tick species parasitizing the Arkansas feral hog populations or the pathogens they carry. This is a public health concern because feral hogs occupy often same geographical regions as humans and livestock and can harbor over 45 animal diseases and parasites. The ticks carried by feral hogs can carry tick-borne pathogens that can cause …

Role Of Reptile-Invertebrate Interactions In Enigmatic Reptile Declines, Meredith Swartwout

Graduate Theses and Dissertations

Reptile populations are declining world-wide and the mechanisms behind many of these declines remain enigmatic. Food web interactions (i.e., reduced prey availability or increased predation) have been implicated behind some reptile declines. However, relatively little is known about predation on lizard and snake eggs, despite egg survival being important for population dynamics of some species. Ants are important predators of squamate reptile eggs in tropical and temperate systems. In Costa Rica, long-term declines in terrestrial anole lizards were linked with reduced leaf litter depth, a factor that could influence egg vulnerability to ant predation. Fire ants (genus Solenopsis) are aggressive …

Climate-Driven Impacts On Himalayan Aquatic Biodiversity: A Case Study Involving Snowtrout (Cyprinidae: Schizothorax), Riri Wiyanti Retnaningtyas

Graduate Theses and Dissertations

Monitoring biodiversity, to include its relative dispersal and contraction, has become a conservation task of great importance, particularly given the catastrophic and ongoing loss of habitat due to climate change. However, the timing, direction, and magnitude of these rates vary across taxa and ecosystems. Predicting specific impacts of climate change can thus be difficult and this, in turn, hampers management action. Metrics are needed to not only quantify contemporary requirements of species, but also predict potential distributions that fluctuate in lockstep with climate.

Montane ecosystems in the Himalayas are highly impacted by climate change, yet remain largely understudied due to …

Influence Of Local- And Landscape-Scale Factors On Avian Assemblage In Fragmented Tallgrass Prairie Landscape, Pooja Panwar

Graduate Theses and Dissertations

Agricultural expansion and increasing urbanization are driving rapid landscape modification in the Tallgrass prairies ecosystem and are affecting biodiversity at multiple spatial scales. Thus, the conservation of fragmented grassland and steeply declining grassland avifauna is dependent on the relationship between local- and landscape-scale factors and avian assemblage. We also need to understand how these factors, operating at different spatial scales, drive the occupancy of different guilds in the assemblage. We conducted repeated point count surveys during the breeding season of 2019 at 66 grassland sites across Arkansas and Missouri, USA. We developed a multi-species occupancy model to estimate species richness, …

Evaluation Of Nutritional Factors That Influence The Efficacy Of Tributyrin As A Feed Additive For Broilers, Brooke Bodle

Graduate Theses and Dissertations

Tributyrin (TB) is a glyceride ester of butyrate that has the potential to improve broiler performance and intestinal development. Therefore, to fully evaluate this potential, three experiments were conducted to evaluate effect of tributyrin (TB) on broiler growth performance, nutrient digestibility, carcass characteristics, intestinal morphology, and gastrointestinal function in birds fed diets varying in composition reared in battery cages and floor pens. Experiment 1 explored the supplementation of graded doses of TB and its impact upon growth performance, nutrient digestibility, and carcass characteristics in a step-down program when added to a reduced energy and amino acid diet. A linear reduction …

Quantifying The Leaching Of Nutrients And Metals In A Pasture Soil As Affected By Excreta Of Ruminants Fed Alternative Diets, Ian Kennedy

Graduate Theses and Dissertations

The leaching of nitrate through the soil profile and into adjacent water systems has been a leading cause of water pollution and results in the loss of a valuable nutrient from animal production systems. Forage-based animal production systems may be improved through manipulation of animal diets that incorporate tannins and total polyphenolic compounds with feed, increasing the amount of nitrogen (N) retained by an animal/decreasing rates of digestion and altering the N-containing compounds that are excreted and deposited onto soil. Incorporating the forage legumes sericea lespedeza (Lespedeza cuneate [Dumont de Courset] G. Don) and lablab (Lablab purpurues L.) into the …

Relationship Of Underline Scores And Production Traits In Beefmaster Cattle, Bryan R. Kutz

Graduate Theses and Dissertations

The objective of this study was to establish the relationship of underline scores (UDLS), production traits, and conformation scores in Beefmaster cattle born between 1976 through 2008. Records provided by Beefmaster Breeders United (BBU) were analyzed. Certified classifiers approved by the BBU recorded scores. Sizes and shapes of the underlines differentiated the scores (1-4). A score of one was used to designate the least amount of navel flap or sheath and a score of four represents the maximum amount of naval flap or sheath accepted for registration in the BBU. In this study the relationship of the traits was determined …

Methods To Reducing Weaning Stress In Early Weaned Spring Beef Calves, Matthew Daniel Hahn

Graduate Theses and Dissertations

Reducing weaning stress in beef calves can have dramatic benefits to the health, growth, and performance to beef calves post-weaning. Studies have shown that providing creep feed to calves during the pre-weaning period can improve their nutritional status, which can better prepare them for the stresses they will be exposed to during the weaning process. Studies have also shown that two-stage weaning can reduce the stresses associated with weaning. Previous research has suggested that this method resulted in dramatic changes in behavioral signs associated with stress. The objective of this study was to evaluate the effects of two-stage weaning with …

Pathogenicity Of Staphylococcus Agnetis Associated With Lame Broilers With Osteomyelitis, Sura Faris Salih Zaki

Graduate Theses and Dissertations

We developed High Resolution Melt Analysis (HERMA) as a rapid and reliable molecular diagnostic assay for the detection and identification of the main bacterial species recovered from the blood and lesions of bacterial chondronecrosis with osteomyelitis (BCO) from the lame broilers at the University of Arkansas poultry research farm. Also, the present study confirms that raising young birds on suspended wire flooring has been proved to successfully induce lameness attributable to BCO with birds being more susceptible to bacteraemia than those that were raised on litter flooring. The newly described pathogen, Staphylococcus agnetis, has been reported to be overrepresented in …

Examining Pathogenesis And Preventatives In Spontaneous And Staphylococcus-Induced Bacterial Chondronecrosis With Osteomyelitis In Broilers, Nnamdi Simon Ekesi

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis is the most important cause of lameness in broilers. This is important to poultry production, as it poses animal welfare issues, and causes a significant loss in revenue. The remediation of this disease requires the study of its etiology with fitting models and evaluating preventatives. The research reported herein covers genomic virulence analysis of BCO isolates, mainly Staphylococcus aureus, and Escherichia coli retrieved from lame birds. We found that S. aureus isolates were closest to chicken strains in Europe but may have been in the Arkansas area for a decade. Phylogenomics suggest our S. aureus is …

Effects Of Long-Term Variation In Temperature On Reproductive Phenology In A Population Of Eastern Bluebirds (Sialia Sialis), Paul Pleiman

Graduate Theses and Dissertations

This study investigates the relationship between multiple temperature variables, to include annual and pre-lay date temperatures with first-egg and mean first-egg lay dates of the eastern bluebird at the Warner Parks in Nashville, Tennessee, USA. Data is collected by citizen scientists for the Eastern Bluebird Nesting Box Project while visiting artificial nest boxes throughout the park and recording observations made during the breeding season. Temperature data is retrieved from the Northwest Alliance for Computational Science and Engineering’s Parameter-elevation Regressions on Independent Slopes Model (PRISM) Climate Group, based at Oregon State University. The analyses showed no correlation between annual or pre-lay …

Fate And Dissemination Of Salmonella Reading In Market-Age Turkeys At Processing Using Oral Gavage Challenge Model, Aaron Ashcraft

Graduate Theses and Dissertations

This study aimed to evaluate the fate and dissemination of Salmonella Reading (SR) in market-age turkeys using an oral gavage challenge model. One hundred twenty-eight-week-old commercial turkey hens were moved from commercial production to research facilities. Upon arrival, a combination of enrofloxacin, 10 mg/kg, and florfenicol, 20 mg/kg, were orally administered sequentially before comingled placement on fresh pine shavings. Turkeys were challenged with 108 cfu SR by oral gavage on days 4 and 7 post-placement. Subsets were subjected to simulated commercial processing on days 14 (n=40), 21 (n=40) and 28 (n=32) post-placement (corresponding to 10, 11, and 12 weeks of …

Evaluation Of Selected Bacillus Direct-Fed Microbial Candidates In Reduced Energy Diets On Live Performance, Carcass Characteristics, And Foot Pad Dermatitis, Kyle Teague

Graduate Theses and Dissertations

Bacillus spp. are ubiquitous, Gram-positive, spore forming bacteria that are commonly recovered from the environment and gastrointestinal tract (GIT) of poultry. These spores are capable of withstanding harsh condition such as feed pelletization, which facilitates inclusion in poultry feeds. Once ingested by the bird, spores germinate into metabolically active vegetative cells which can produce extracellular enzymes which can hydrolyze otherwise indigestible components of the feed. Soybean meal (SBM) is the gold standard vegetable protein source for non-ruminant animals worldwide and is included in practically all poultry diets in the United States at levels ranging from 10 to more than 30%. …

Effect Of Feed Additives And Toxic Elements On Swine Growth Performance, Nutrient Digestibility, Immune Function And Reproductive Performance, Richard A. Mudarra Hernández

Graduate Theses and Dissertations

To evaluate the effects of peptide in combination of zinc oxide (Zno) or acidifiers (Exp 1), and gossypol (G) from cottonseed meal (CSM) ( Exp 2&3) on growth performance, complete blood cell counts (Exp 1, 2&3), nutrient digestibility (Exp 1), plasma gossypol (Exp 2&3) and semen quality (Exp 3), weaned pigs (Exp 1), growing gilts (Exp 2) and growing boars (Exp 3) were randomly allotted to dietary treatments. Treatments for Exp 1 during phase 1&2 were: (1) Positive Control (PC), formulated to meet NRC (2012) nutrient requirements; (2) Negative control (NC), fish meal was reduced to achieve -0.13% SID lysine; …

Evaluation Of Different Ensiling Methods On Storage And Feeding Value Of The Residual Material From Edamame Soybean Processing, Ellen Herring

Graduate Theses and Dissertations

Use of organic waste material by ruminant animals from food processing operations potentially reduces costs and reduces environmental issues from disposal of these residues. Therefore, 2 experiments were conducted to evaluate the storage and feeding value of residual from edamame soybean processing for ruminant animals. Two types of waste streams, waste during harvest time and waste from processing stored material, were ensiled (on a laboratory scale) using various methods and effects on post-ensiling nutritive value were examined. Material from both waste streams were ensiled either without wilting or after wilting; each moisture level was ensiled with and without an inoculant. …

Effects Of Commercial Feed Additives On Growth Performance And Gut Microbiota Of Nursery Pigs, Xiaoyuan Wei

Graduate Theses and Dissertations

To secure animal welfare and maximum production, feed additives are often added to nursery pig diets to increase nutrition digestibility, enhance intestinal health, and prevent or mitigate disease, with the ultimate goal of improving growth performance. This thesis performed a longitudinal analysis using next-generation sequencing to investigate the dynamic changes of gut microbiota in weaned pigs fed commercial feed additives. Chapter II was used to evaluate the effects of organic acid mixture on growth performance and gut microbiota of weaning pigs. This study demonstrated that the inclusion of 0.035% (SBA0.035) or 0.070% (SBA0.070) sodium butyrate in a diet containing 0.5% …

Biogeography Of The Bovine Respiratory Microbiome And Its Relationship With Bovine Respiratory Disease, Jianmin Chai

Graduate Theses and Dissertations

Bovine respiratory disease (BRD) is the most common and costly disease in the beef cattle industry, leading to high morbidity, mortality and huge economic loss. Despite the recent advances in vaccination and antimicrobial techniques, no significant health-improved outcomes have developed. Due to a deep investigation of the microbiome, respiratory microbiotas are known to have important roles for host health and disease. However, BRD specific pathogens have not yet been identified since they are found in both healthy and diseased animals. A systemic and comprehensive study of the biogeography of the bovine respiratory microbiome and its relationship with BRD is lacking …