Botany Commons^™

Engineering Linear, Branched-Chain Triterpene Metabolism In Monocots, Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J. Sato, Zhengxiang Ge, Thomas E. Clemente, Joe Chappell

Department of Agronomy and Horticulture: Faculty Publications

Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to yield farnesyl diphosphate (FPP), catalyzed by FPP synthase (FPS). Squalene synthase (SQS) condenses two molecules of FPP to generate the symmetrical product squalene, the first committed precursor to sterols and most other triterpenes. In the green algae Botryococcus braunii, two FPP molecules can also be condensed in an asymmetric manner yielding the more highly branched triterpene, botryococcene. Botryococcene …

Genomic Selection In Preliminary Yield Trials In A Winter Wheat Breeding Program, Vikas Belamkar, Mary J. Guttieri, Waseem Hussain, Diego Jarquin, Ibrahim El-Basyoni, Jesse Poland, Aaron J. Lorenz, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Genomic prediction (GP) is now routinely performed in crop plants to predict unobserved phenotypes. The use of predicted phenotypes to make selections is an active area of research. Here, we evaluate GP for predicting grain yield and compare genomic and phenotypic selection by tracking lines advanced. We examined four independent nurseries of F_3:6 and F_3:7 lines trialed at 6 to 10 locations each year. Yield was analyzed using mixed models that accounted for experimental design and spatial variations. Genotype-by-sequencing provided nearly 27,000 high-quality SNPs. Average genomic predictive ability, estimated for each year by randomly masking lines as missing …

Transient Heat Stress During Early Seed Development Primes Germination And Seedling Establishment In Rice, Kevin Begcy, Jaspreet Sandhu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Rice yield is highly sensitive to increased temperature. Given the trend of increasing global temperatures, this sensitivity to higher temperatures poses a challenge for achieving global food security. Early seed development in rice is highly sensitive to unfavorable environmental conditions. Heat stress (HS) during this stage decreases seed size and fertility, thus reducing yield. Here, we explore the transgenerational phenotypic consequences of HS during early seed development on seed viability, germination, and establishment. To elucidate the impact of HS on the developmental events in postzygotic rice seeds, we imposed moderate (35°C) and severe (39°C) HS treatments initiated 1 day after …

Investigating The Ecology Of A Rare Species On St. John, Usvi: Reintroducing Solanum Conocarpum In Light Of Climate Change, Cecilia Rogers

Senior Honors Projects, 2010-2019

Approximately two thirds of St. John is National Park territory. However, the land has been threatened with tourism and development, greatly impacting island biodiversity. One species that may become extinct due to this degradation is Solanum conocarpum. S. conocarpum is a rare shrub, endemic to the dry tropical forests of St. John, USVI. This plant is a species of conservation concern and is one of very few native and endemic plants on this island. Very little is known about the ecology and reproduction of S. conocarpum. Most plants are found on the southern half of the island. Recent observations …

Low- Copy Nuclear Markers In Isoëtes (Isoëtaceae) Identified With Transcriptomes, Peter W. Schafran, Gabriel Johnson, W. Carl Taylor, Elizabeth A. Zimmer, Lytton J. Musselman

Biological Sciences Faculty Publications

PREMISE OF THE STUDY: Few genetic markers provide phylogenetic information in closely related species of Isoëtes (Isoëtaceae). We describe the development of primers for several putative low- copy nuclear markers to resolve the phylogeny of Isoëtes, particularly in the southeastern United States.

METHODS AND RESULTS: We identified regions of interest in Isoëtes transcriptomes based on low- copy genes in other plants. Primers were designed for these regions and tested with 16 taxa of Isoëtes and one species of Lycopodium. Parts of the pgiC, gapC, and IBR3 gene regions show phylogenetic signal within the North American and …

A New Anatomically-Preserved Plant From The Lower Devonian Of Quebec (Canada): Implications For Euphyllophyte Phylogeny And Early Evolution Of Structural Complexity, Selin Toledo

Cal Poly Humboldt theses and projects

An abrupt transition in the fossil record between Early Devonian plants with simple structure and structurally-complex later Devonian plants, has frustrated efforts to understand patterns of phylogeny across the Early/Middle Devonian boundary and the evolution of complex forms. Both these aspects have important implications for lignophyte and seed plant evolution. In the first chapter, I evaluate phylogenetic relationships between the earliest seed plants, Aneurophytales, and Stenokoleales, using comprehensive taxon sampling (28 species, including all relevant permineralized species) and a set of 40 discrete and nine continuous morpho-anatomical characters. Analysis of this dataset supports the three traditional taxonomic groups (seed plants, …

Effects Of Exogenous Salicylic Acid And Sodium Nitroprusside On α-Tocopherol And Phytochelatin Biosynthesis In Zinc-Stressed Safflower Plants, Shahram Namdjoyan, Hossein Kermanian, Ali Abolhasani Soorki, Sedigheh Modarres Tabatabaei, Nazli Elyasi

Turkish Journal of Botany

The interactive effect of exogenous application of salicylic acid (SA) and sodium nitroprusside (SNP), a donor of nitric oxide, on zinc (Zn) toxicity was assessed in Zn-stressed safflower (Carthamus tinctorius L.) seedlings. Exposure to 500 μM ZnSO4.7H2O for 10 days caused an increment in the levels of malondialdehyde (MDA), H2O2, α-tocopherol, phytochelatins (PCs), and proline. Application of SA or SNP, and especially their combination, resulted in remarkable decrease in MDA, H2O2, and proline content while chlorophyll content and the activity of catalase, ascorbate peroxidase, and guaiacol peroxidase exhibited a significant induction in comparison with plants subjected to Zn treatment alone. …

Primula Veris Plants Derived From In Vitro Cultures And From Seeds: Genetic Stability, Morphology, And Seed Characteristics, Iwona Jedrzejczyk, Maria Morozowska, Renata Nowinska, Andrzej Jagodzinski

Turkish Journal of Botany

Flow cytometry measurement confirmed the genetic stability of Primula veris L. (cowslip) plants obtained during the micropropagation process. The mean 2C DNA content was 0.97 pg/2C and P. veris can be classified in the group of plants of very small genomes. Comparative morphological analysis of cowslip plants regenerated in vitro and derived from seeds was carried out in three subsequent years under common garden conditions, which limited the effects of environmental factors. The origin of specimens significantly influenced most of the morphological traits examined. Plants that originated from seeds were characterized by more intensive development rate and higher total seed …

Callus Production And Analysis Of Some Secondary Metabolites In Globularia Trichosantha Subsp. Trichosantha, Hati̇ce Çölgeçen, Havva Atar, Gülnur Toker, Gençay Akgül

Turkish Journal of Botany

This study describes the production of calli from Globularia trichosantha subsp. trichosantha and the quantitative determination of catalpol, aucubin, and verbascoside in the calli and the plant by HPLC. The seeds of the plant were sterilized and germinated in Murashige and Skoog (MS) medium without in vitro plant growth regulator. Hypocotyl, cotyledon, first leaf, epicotyl, apical meristem, and root explants were taken from the 30-day-old aseptic seedlings germinated in vitro. Explants were then transferred to MS media for callus production together with varying concentrations of plant growth regulators. The best callus production occurred in the media containing 6 mg L?1 …

Genomic-Enabled Prediction Models Using Multi-Environment Trials To Estimate The Effect Of Genotype × Environment Interaction On Prediction Accuracy In Chickpea, Manish Roorkiwal, Diego Jarquin, Muneendra K. Singh, Pooran M. Gaur, Chellapilla Bharadwaj, Abhishek Rathore, Reka Howard, Samineni Srinivasan, Ankit Jain, Vanika Garg, Sandip Kale, Annapurna Chitikineni, Shailesh Tripathi, Elizabeth Jones, Kelly R. Robbins, José Crossa, Rajeev K. Varshney

Department of Agronomy and Horticulture: Faculty Publications

Genomic selection (GS) by selecting lines prior to field phenotyping using genotyping data has the potential to enhance the rate of genetic gains. Genotype × environment (G × E) interaction inclusion in GS models can improve prediction accuracy hence aid in selection of lines across target environments. Phenotypic data on 320 chickpea breeding lines for eight traits for three seasons at two locations were recorded. These lines were genotyped using DArTseq (1.6 K SNPs) and Genotyping-by-Sequencing (GBS; 89 K SNPs). Thirteen models were fitted including main effects of environment and lines, markers, and/or naïve and informed interactions to estimate prediction …

Gene Expression Profiling Of Iron Deficiency Chlorosis Sensitive And Tolerant Soybean Indicates Key Roles For Phenylpropanoids Under Alkalinity Stress, Brian M. Waters, Keenan Amundsen, George L. Graef

Department of Agronomy and Horticulture: Faculty Publications

Alkaline soils comprise 30% of the earth and have low plant-available iron (Fe) concentration, and can cause iron deficiency chlorosis (IDC). IDC causes soybean yield losses of $260 million annually. However, it is not known whether molecular responses to IDC are equivalent to responses to low iron supply. IDC tolerant and sensitive soybean lines provide a contrast to identify specific factors associated with IDC.We used RNA-seq to compare gene expression under combinations of normal pH (5.7) or alkaline pH (7.7, imposed by 2.5mM bicarbonate, or pH 8.2 imposed by 5mM bicarbonate) and normal (25μM) or low (1μM) iron conditions from …

Managing Grazing Lands To Improve Soils And Promote Climate Change Adaptation And Mitigation: A Global Synthesis, Marcia Delonge, Andrea D. Basche

Department of Agronomy and Horticulture: Faculty Publications

The potential to improve soils to help farmers and ranchers adapt to and mitigate climate change has generated significant enthusiasm. Within this discussion, grasslands have surfaced as being particularly important, due to their geographic range, their capacity to store substantial quantities of carbon relative to cultivated croplands and their potential role in mitigating droughts and floods. However, leveraging grasslands for climate change mitigation and adaptation will require a better understanding of how farmers and ranchers who rely on them for their livelihoods can improve management and related outcomes. To investigate opportunities for such improvements, we conducted a meta-analysis of field …

Interesting, Provocative, And Enigmatic: Morphological Observations On Southeastern Quillworts (Isoetes Isoetaceae, Lycopodiophyta, Rebecca D. Bray, Peter W. Schafran, Lytton J. Musselman

Biological Sciences Faculty Publications

The unique morphology of quillworts has received limited attention, and such studies have been limited to only a few species. Our extensive field work during the past two decades has revealed variation in the structures of the plant, including the rhizomorph, scales, phyllopodia, and abscission caps. Polarity of the axes of southeastern quillwort rhizomorphs varies from discoid (most species), to elongate, to upright with axis branching in several species. In species of intermittent streams, these branched rhizomorphs produce plantlets that break off in running water. Scales are tiny brown or black structures in alternating whorls with sporophylls (leaves). They are …

Plant Community Response To Disturbances In Nebraska Sandhills Upland Prairie, Josiah D. Dallmann

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Historically, disturbances shaped prairie ecosystems both directly through events like fire and soil moving by animals, as well as indirectly through dynamic rainfall patterns and periodic flux in seed availabilities. We hypothesized that the implementation of several distinct disturbance events in Nebraska Sandhills upland prairie would have measurable effects on plant community composition. We implemented mid-spring fire, soil disturbance in the form of disking, seed addition of native forbs on disked plots, as well as early summer supplemental watering (to mimic minor rainfall events during abnormally dry periods) during 2016 and 2017. Plant community response during the first and second …

Plastid Dna Variation Of The Endemic Species Oxytropis Glandulosa Turcz. (Fabaceae), Alla Kholina, Marina Kozyrenko, Elena Artyukova, Denis Sandanov, Inessa Selyutina, Daba Chimitov

Turkish Journal of Botany

Oxytropis glandulosa Turcz. (Fabaceae) is a rare perennial plant endemic to Buryatia (Russia). We sequenced three intergenic spacers (the psbA−trnH, trnL−trnF, and trnS−trnG) of chloroplast DNA (cpDNA) in specimens of four populations from two geographic regions (Barguzin and Yeravna depressions) within this species range. The levels of haplotype and nucleotide diversity varied in the range of 0.133−0.911 and 0.0002−0.0059, respectively. The highest values of these parameters are characteristic of populations located in the Barguzin depression. Variable sites detected within the intergenic spacers allowed the identification of eleven haplotypes; nine of them were found in the populations from the Barguzin depression …

Predicting The Potential Distribution Area Of Solidago Xniederederi (Asteraceae), Malgorzata Jazwa, Elzbieta Jedrzejczak, Ewelina Klichowska, Artur Pliszko

Turkish Journal of Botany

In this study, we aimed to investigate the potential distribution area of Solidago xniederederi, a natural hybrid between North American S. canadensis and European S. virgaurea, in Central and East Europe using the MAXENT modeling approach. The final MAXENT model was constructed based on 83 occurrence records from Austria, Poland, Lithuania, and Latvia and six abiotic environmental variables. The jackknife test revealed that annual temperature range, mean temperature of wettest quarter, and minimum temperature of coldest month had the highest gain for the training and test data when used alone, whereas precipitation seasonality, precipitation of coldest quarter, and precipitation of …

Xanthagaricus Pakistanicus Sp. Nov. (Agaricaceae): First Report Of The Genus From Pakistan, Shah Hussain, Najam-Ul-Sahar Afshan, Habib Ahmad, Hassan Sher, Abdul Khalid

Turkish Journal of Botany

Xanthagaricus pakistanicus is described as a new species from lowland northern Pakistan, based on morphological and molecular data. It is characterized by a yellowish pileus, covered with dark brown squamules, a stipe with yellowish fibrils, globose basidiospores, and pileal squamules made up of pseudoparenchymatous epithelium with encrusted walls. Molecular phylogenetic trees were inferred based on nucleotide sequences of the internal transcribed spacer (ITS1-5.8S-ITS2) region and 28S nuclear ribosomal DNA. In phylogenetic analyses the genus Hymenagaricus sensu lato (s. l.) was inferred as a nonmonophyletic group and recovered in two monophyletic clades, consisting of species of the genera Xanthagaricus and Hymenagaricus …

Properties, Variations, Roles, And Potential Applications Of Epicuticular Wax: A Review, Priyal Sharma, Shankar Lal Kothari, Mahender Rathore, Vinod Gour

Turkish Journal of Botany

The cuticular wax layer covers the aerial surface of plants and acts as a barrier between plants and the environment. The cuticle plays a key role in the protection of plants from pathogens, UV light, and transpiration. Variation in the wax quality and quantity is influenced by factors like the solvent used for extraction, species, ontogeny, and season. Compounds isolated from the cuticle layer have been studied by various methods and were found to play an important role from the ecological and physiological points of view. These compounds include esters, alcohols, ether, alkane, and aldehydes. Nonpolar compounds help reduce water …

Phenotypic Evaluation Of Transgenic Peas (Pisum Sativum L.) Harboring Atnhx1 Demonstrates Stable Gene Expression And Conserved Morphology In Subsequent Generations, Zahid Ali, Wajeeha Saeed, Saadia Naseem, Faheem Ahmad, Ahmed Akrem, Nighat Yasmeen, Hans Joerg Jacobsen

Turkish Journal of Botany

In recent years, transgenic approaches have played a significant role in improving traits that help plants overcome abiotic stresses. Combined gene discovery and functional genomics have helped identify diversified mechanisms and gene families, which improved productivity under various abiotic stresses. We report on the genetic stability and persistent morphological features of transgenic pea (Pisum sativum L.) plants harboring the dicistronic vector construct pG0229MASnhx1/luc over five generations. In addition to salt stress tolerance, the transgenic plants showed frost tolerance compared to wild-type (WT) plants. Frost tolerance of AtNHX1 transgenic pea plants was unexpected and needs further investigation. We report morphological and …

Morphological, Chemical, And Genetic Diversity Of Gypsophila L. (Caryophyllaceae) Species And Their Potential Use In The Pharmaceutical Industry, Barbara Kolodziej, Sylwia Okon, Aleksandra Nucia, Tomasz Ociepa, Katarzyna Luchowska, Danuta Sugier, Reneta Gevrenova, Max Henry

Turkish Journal of Botany

The aim of this study was to evaluate the morphological, chemical, and genetic similarity of species belonging to the genus Gypsophila L. The analysis included 7 Gypsophila species with potential use in the pharmaceutical industry for saponin production. In order to assess the variation of the morphological traits, a number of morphological characteristics have been determined (including the number of lateral roots, diameter and total root length, average plant height, number of shoots and their diameter, number of branches on the shoot, length and width of leaves, and fresh and air-dried weight of aerial and underground parts of plants), while …

Monitoring An Endangered And Rare Plant: Population Growth And Viability Oflotus Kunkelii (Esteve) Bramwell And Davis (Gran Canaria - Canary Islands), Agustin Naranjo Cigala, Marcos Salas, Francisco Javier Diaz, Aday Miguel Gonzalez-Garcia, Ana Ramos, Jose Ramon Arevalo Sierra

Turkish Journal of Botany

Species monitoring is a valuable tool that can be used to answer a wide range of questions directly related to species conservation, management, and population improvement. We monitored the only remaining population of Lotus kunkelii on a monthly basis over 3 years to reveal important characteristics of the population that could help protect it. In this study, the main hypothesis to be tested is whether the population of L. kunkelii is stable or not. The study site is located in Gran Canaria. Monthly censuses of the population recording size (height and diameter), mortality, regeneration, and presence of reproductive structures in …

New Additions To The Turkish Pezizales, Yasi̇n Uzun, Semi̇ha Yakar, İbrahi̇m Hali̇l Karacan, Abdullah Kaya

Turkish Journal of Botany

Twelve members of the order Pezizales are recorded for the first time from Turkey: Ascobolus carbonarius P.Karst.; A. crenulatus P.Karst.; A. foliicola Berk. & Broome; A. immersus Pers.; Saccobolus glaber (Pers.) Lambotte; Thecotheus holmskioldii (E.C.Hansen) Eckblad (Ascobolaceae Boud. ex Sacc.); Lasiobolus cuniculi Velen. (Ascodesmidaceae J.Schröt.); Barssia hellenica Kaounas, Agnello, P.Alvarado & Slavova (Helvellaceae Fr.); Marcelleina atroviolacea Brumm.; M. rickii (Rehm) Graddon (Pezizaceae Dumort.); Cheilymenia pulcherrima (P.Crouan & H.Crouan) Boud.; and Sepultariella semi-immersa (P.Karst.) Van Vooren, U.Lindem. & Healy (Pyronemataceae Corda). Three of them, S. glaber (Pers.) Lambotte; B. hellenica Kaounas, Agnello, P.Alvarado & Slavova; and S. semi-immersa (P.Karst.) Svrcek, are …

Host-Driven Morphological Variability In Orobanche Crenata (Orobanchaceae), Gianniantonio Domina

Turkish Journal of Botany

Studies on interactions between Orobanche species and their hosts are mainly focused on resistance, radical interactions, and haustorium development. The influence of the host plant on morphology of the parasite under environmental controlled conditions is not reported in the literature. With the aim of filling this gap, the results of cultivation experiments of Orobanche crenata on Vicia faba, V. sativa, Pisum sativum, Cicer arietinum, and Lathyrus clymenum are presented. The tested population of O. crenata infected all the studied hosts but showed preferences toward Vicia and Pisum and, in particular, toward a foreign V. sativa cv. The higher resistance of …

Ekimia Ozcan-Secmenii (Apiaceae), A New Species From Southwest Anatolia, Turkey, Serdar Gökhan Şenol, Volkan Eroğlu, Nazli Bahar Peli̇t, Duygu Bozyel

Turkish Journal of Botany

Ekimia ozcan-secmenii Şenol & Eroğlu sp. nov. (Apiaceae) is described from SW Turkey. E. ozcan-secmenii differs from its close affinity, E. bornmuelleri, by life form (monocarpic), petal and bracteole morphology, and mericarp features. These data were more supported by the results of nrDNA ITS sequences. The ML tree depicted based on phylogenetic studies aimed to reveal the closeness of the Laserpitium and Prangos species as well as the location the affinity of species in the genus Ekimia. The taxonomic situation of Prangos hulusi was also evaluated based on nrDNA ITS sequences. Since E. ozcan-secmenii is distributed in Sami Soydam Sandalcık …

The Epilithic And Epipelic Diatom Flora Of The Balikhli River, Northwest Iran, Jamileh Panahy Mirzahasanlou, Taher Nejadsattari, Zohreh Ramezanpour, Javid Imanpour Namin, Younes Asri

Turkish Journal of Botany

The taxonomic composition of diatom flora of the Balikhli River (Balikhli Chay), Northwest Iran, was studied from October 2013 through September 2014. In total 109 taxa belonging to 50 genera were identified. Nitzschia (18 spp.), Navicula (9 spp.), and Gomphonema (7 spp.) were the most abundant genera. Twenty-five diatom species were found in more than half of the samples; among them, Nitzschia inconspicua, Planothidium lanceolatum, Amphora pediculus, Navicula trivialis, Gomphonema olivaceum, Nitzschia palea, and Rhoicosphenia abbreviata were the most abundant taxa, which are characteristic for alkaline and eutrophic waters. The centric diatoms constituted only 4.54% of all identified taxa. In …

New Records And Rare Taxa For The Freshwater Algae Of Turkeyfrom The Tatar Dam Reservoir (Elazığ), Memet Varol, Saul Blanco, Kenan Alpaslan, Gökhan Karakaya

Turkish Journal of Botany

Recently, the number of algological studies in Turkish inland waters has increased remarkably. However, taxonomic and floristic studies on algae in the Euphrates basin are still scarce. This study contributes new information to the knowledge of the Turkish freshwater algal flora. Phytoplankton samples were collected from the Tatar Dam Reservoir in the Euphrates Basin between January 2016 and December 2016. Two taxa were recorded for first time and 14 rare taxa for the freshwater algae of Turkey were identified in this study. The new records belong to the phylum Bacillariophyta, whereas taxa considered as rare belong to the phyla Chlorophyta, …

Overexpression Of The Glycine Max Chalcone Isomerase (Gmchi) Gene In Transgenictalinum Paniculatum Plants, Thi Nhu Trang Vu, Thi Hong Trang Le, Phu Hiep Hoang, Danh Thuong Sy, Thi Thu Thuy Vu, Hoang Mau Chu

Turkish Journal of Botany

Talinum paniculatum plants contain flavonoids and saponins, which have strong antioxidant properties and are used in the treatment of numerous diseases, such as inflammation, allergies, and gastric ulcers. Currently, there is no published research on the flavonoid content of T. paniculatum plants; however, it has been determined that the species in the genus Talinum have very low flavonoid content, and therefore the effective approach to enhance the flavonoid content in T. paniculatum plants is overexpression of the Glycine max chalcone isomerase (GmCHI) gene. In this study, we analyzed GmCHI gene overexpression, which has increased total flavonoid content in transgenic T. …

Two New Malagasy Species Of Genus Piper L. (Piperaceae), Piper Malgassicum And Piper Tsarasotrae, And Their Phylogenetic Position, Enrico Palchetti, Stefano Biricolti, Massimo Gori, Giulia Rota Nodari, Nicola Gandolfi, Alessio Papini

Turkish Journal of Botany

Here we describe two new species of genus Piper L. from Madagascar: Piper malgassicum and Piper tsarasotrae, the species names referring to the currently known distribution areas. These two species contribute, at least in part, to the production of local voatsiperifery pepper, probably mixed together with P. borbonense, and are therefore economically relevant as spices. We used a selected set of characters (those more easily observable on herbarium samples) for principal component analysis to assess the relative distance between the species, including the analysis of the autochthonous species of Piper known from Africa and Madagascar. In order to check the …

New Contributions To The Turkish Ascomycota, Abdullah Kaya, Yasi̇n Uzun

Turkish Journal of Botany

Nine discomycete and one sordariomycete (Ascomycota) species are reported for the first time from Turkey. The genera Coccomyces, Kompsoscypha, Pseudopithyella, Strobiloscypha, and Lasiosphaeris have not been reported before in the country. Anthracobia, Plicaria, Sclerotinia, and Pithya species are new records added to the previous knowledge. Macro- and micromorphological descriptions and illustrations for each new taxon are provided.

Seed Structure Of Some Taxa Of The Genus Crocus L. (Iridaceae) Series Crocus, Mehmet Cengi̇z Karai̇smai̇loğlu, Levent Şik, Almila Gemi̇ci̇oğlu, Osman Erol

Turkish Journal of Botany

In this study, the macro- and micromorphological seed characteristics of the genus Crocus L. series Crocus taxa, including C. pallasii , C. turcicus , C. dispathaceus , C. asumaniae , C. mathewii , C. thomasii , C. oreocreticus , and C. cartwrightianus , were observed. Morphological characteristics, such as the size, shape, color, and seed surface structures, were analyzed using stereo and scanning electron microscopes. Testa layer structures including the outer and inner testae, phytomelan layer structure and thickness, and seed endosperm cell features were studied. Differences in the seed, raphe size, seed surface patterns, inner testa features, and phytomelan …