Botany Commons^™

Genomics-Assisted Characterization Of A Breeding Collection Of Apios Americana, An Edible Tuberous Legume, Vikas Belamkar, Andrew Farmer, Nathan T. Weeks, Scott R. Kalberer, William J. Blackmon, Steven B. Cannon

Department of Agronomy and Horticulture: Faculty Publications

For species with potential as new crops, rapid improvement may be facilitated by new genomic methods. Apios (Apios americana Medik.), once a staple food source of Native American Indians, produces protein-rich tubers, tolerates a wide range of soils, and symbiotically fixes nitrogen. We report the first high-quality de novo transcriptome assembly, an expression atlas, and a set of 58,154 SNP and 39,609 gene expression markers (GEMs) for characterization of a breeding collection. Both SNPs and GEMs identify six genotypic clusters in the collection. Transcripts mapped to the Phaseolus vulgaris genome–another phaseoloid legume with the same chromosome number–provide provisional genetic …

Plants Having Increased Bomass And Methods For Making The Same, Aleel K. Grennan, Donald R. Ort, Stephen Patrick Moose, Damla D. Bilgin, Thomas Clemente, Fredy Altpeter, Stephen P. Long

Department of Agronomy and Horticulture: Faculty Publications

The impact of plastid size change in both monocot and dicot plants has been examined. In both, when plastid size is increased there is an increase in biomass relative to the parental lines. Thus, provided herein are methods for increasing the biomass of a plant, comprising decreasing the expression of at least one plastid division protein in a plant. Optionally, the level of chlorophyll in the plant is also reduced.

Studying The Effects Of Serpentine Soil On Adapted And Non-Adapted Species Using Arduino Technology, Kiana Saniee, Edward Himelblau, Brian Paavo

STAR Program Research Presentations

Abstract: Serpentine soils are formed from ultramafic rocks and are represent an extreme environment for plants. Serpentine soils are unique in that they carry high concentrations of heavy metals, are nutrient deficient, particularly in calcium, and have poor water retention capabilities. Although these soils constitute harsh conditions for plant growth, there are a number of species that are adapted and even endemic to serpentine soil. Water retention by commercial potting mix was compared with serpentine soil. Also, serpentine adapted and non-adapted species were grown in both soil treatments and physiological data were collected. We used the Arduino electronic platform to …

Characterization Of Echinochloa Spp. In Arkansas, Hussain Tahir

Graduate Theses and Dissertations

Echinochloa species are the most problematic grass weeds in rice and soybean production throughout the southern United States. The species under this genus has high intra- and interspecific variability, with many ecotypes, observed within a species. This study was conducted to: i) characterize the morphology and phenology of Echinochloa accessions from Arkansas in a common garden; ii) verify the species identity of these accessions based on their morphological traits, and iii) evaluate the dormancy characteristics of Echinochloa accessions. Junglerice (E. colona) was identified as the most common species, comprising about 78% of the accessions collected, with barnyardgrass (E. crus-galli) and …

Variation In Leaf Wettability Traits Along A Tropical Montane Elevation Gradient, Gregory R. Goldsmith, Lisa Patrick Bentley, Alexander Shenkin, Norma Salinas, Benjamin Blonder, Roberta E. Martin, Rosa Castro-Ccossco, Percy Chambi-Porroa, Sandra Diaz, Brian J. Enquist, Gregory P. Asner, Yadvinder Malhi

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

• Leaf wetting is often considered to have negative effects on plant function, such that wet environments may select for leaves with certain leaf surface, morphological, and architectural traits that reduce leaf wettability. However, there is growing recognition that leaf wetting can have positive effects.
• We measured variation in two traits, leaf drip tips and leaf water repellency, in a series of nine tropical forest communities occurring along a 3300-m elevation gradient in southern Peru. To extend this climatic gradient, we also assembled published leaf water repellency values from 17 additional sites. We then tested hypotheses for how these traits should …

Leaf Protein And Mineral Concentrations Across The “Miracle Tree” Genus Moringa, Mark E. Olson, Renuka P. Sankaran, Jed W. Fahey, Michael A. Grusak, David Odee, Wasif Nouman

Publications and Research

The moringa tree Moringa oleifera is a fast-growing, drought-resistant tree cultivated across the lowland dry tropics worldwide for its nutritious leaves. Despite its nutritious reputation, there has been no systematic survey of the variation in leaf nutritional quality across M. oleifera grown worldwide, or of the other species of the genus. To guide informed use of moringa, we surveyed protein, macro-, and micro- nutrients across 67 common garden samples of 12 Moringa taxa, including 23 samples of M. oleifera. Moringa oleifera, M. concanensis, M. stenopetala, an M. concanensis X oleifera hybrid, and M. longituba were highest in protein, with M. …

Cross Species Selection Scans Identify Components Of C4 Photosynthesis In The Grasses, Pu Huang, Anthony J. Studer, James C. Schnable, Elizabeth A. Kellogg, Thomas P. Brutnell

Department of Agronomy and Horticulture: Faculty Publications

C₄ photosynthesis is perhaps one of the best examples of convergent adaptive evolution with over 25 independent origins in the grasses (Poaceae) alone. The availability of high quality grass genome sequences presents new opportunities to explore the mechanisms underlying this complex trait using evolutionary biology-based approaches. In this study, we performed genome-wide cross-species selection scans in C₄ lineages to facilitate discovery of C₄ genes. The study was enabled by the well conserved collinearity of grass genomes and the recently sequenced genome of a C₃ panicoid grass, Dichanthelium oligosanthes. This method, in contrast to previous studies, …

Endless Symbioses Most Intricate, W. John Hayden

Biology Faculty Publications

Orchids, such as our Wildflower of the Year for 2016, Downy Rattlesnake Plantain (Goodyera pubescens) (Fig. 1), exemplify the interconnectedness of life on Earth. As would be the case for many kinds of plants, pollination comes readily to mind as a prominent example of mutualistic symbiosis. Downy Rattlesnake Plantain is pollinated by bumblebees and other native bees. The bees gain nectar and the orchid gets an efficient means to move pollen from one flower to another; each organism gains benefit from the interaction, the very definition of mutualism. Pollination by bees is widespread among the orchids, but there …

Herbicide Resistantsorghum Mutants, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

This disclosure provides for four different sorghum mutants that exhibit resistance to ALS-inhibiting herbicides. This dis closure also provides for methods of using such Sorghum mutants that exhibit resistance to ALS-inhibiting herbicides in breeding methods to make Sorghum hybrids, varieties, or lines. The Sorghum hybrids, varieties, and lines provided in this disclosure can be used in methods of controlling weeds.

Mapping Morels: Predicting The Locations Of Morchella Species Through Environmental Factors Using The Gis System, Emily M. Stanevicius

Celebration of Learning

Morel mushrooms, Morechella esculenta and M. deliciosa, are known delicacies across the globe, ranging from exquisite dishes in French cuisine to Eastern palates such as Japanese Matsutake. According to literature, true morels diverged as their own genus about 129 million years, again which has led to the development of more than 177 species and have been part of the human diet since their beginning. However, the elusiveness of morels has contributed to the mushrooms infamy for rarity, and has even been known to sell for more than $40 per pound. This project seeks to aid in the search for morels …

The Effects Of Two Levels Of Salinity On Wisconsin Fast Plants, Megan M. Janssen, Emily Geison, Tiffany Bertoni, Matthew Zimmerman

Celebration of Learning

Wisconsin Fast Plant (Brassica rapa, Brassicaceae) seeds were planted in three, six-celled planting containers filled with potting soil and put beneath a continuous grow-lux light. One week after sprouting, one container (E1) was put into a separate tray filled with 0.5% NaCl solution, another container (E2) was put into a separate tray filled with 1.0% NaCl solution, and the third container was left in regular tap water. At seven-day intervals thereafter, the height of all plants was recorded, and the numbers of leaves and flowers were recorded. By week four of recording data, the average height (mm) of the control …

Effect Of Different Miracle-Gro Concentrations On The Growth Of Wisconsin Fast Plants, Eric D. Shershen, Nicholas M. Orslini, David Channon, Marvin Agyeben

Celebration of Learning

The Wisconsin Fast Plant, Brassica rapa (Brassicaceae) seeds were planted in three, six-celled containers and were filled with standard potting soil under continuous grow-lux lighting. After the first week of plant growth, E1and E2 were placed in a separate trays and given 1.0 and 1.5x the recommended amount of Miracle-Gro formula, respectively. The control was left in tap water and all were kept in 1 cm of solution. Every seven days the plants’ heights were recorded, and the total number of flowers were counted. By the fourth week, the average height of the control plants was 105.7 mm, of the …

Long-Term Sandhills Prairie Responses To Precipitation, Temperature, And Cattle Stocking Rate, John A. Guretzky, Cheryl Dunn, Heidi L. Hillhouse

Department of Agronomy and Horticulture: Faculty Publications

Understanding of Sandhills prairie, the most expansive sand dune region stabilized by perennial grasses in the Western Hemisphere, is limited by lack of long-term vegetation data. We used a 26-year dataset to evaluate Sandhills prairie responses to yearto- year variation in precipitation, temperature, and cattle stocking rate. Basal cover, a measurement that is constant seasonally and used to detect long-term changes in bunchgrass vegetation, was measured in 38–40 permanent plots positioned along four transects spanning 769 ha from 1979 to 2007. Across this period, total basal cover averaged 2.4 % and was dominated by warm-season grasses (81.1 %). Schizachyrium scoparium …

Regeneration: An Overlooked Aspect Of Trait-Based Plant Community Assembly Models, Julie Larson, Jennifer L. Funk

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Despite the disproportionate influence that propagule production, dispersal, seed to seedling recruitment, and vegetative reproduction can have on plant population and community dynamics, progress has been slow in the directed collection of regeneration traits to inform community assembly outcomes.

While seed mass is globally available and linked to growth and reproductive output, there are limits to its explanatory ability. In this essay, we call for expanded efforts to integrate a more diverse set of regeneration traits into community assembly models.

First, we extend an existing community assembly framework to conceptualize regeneration as a series of transitional processes whose outcomes are …

Herbaceous 2016, Julia I. Chapman, Keith E. Gilland, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

Herbaceous vegetation data collected in 2016 at Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.

Heat Stress Effects On Cotton During Reproductive Development And Subsequent Acclimation Responses, Toby Ryan Fitzsimons

Graduate Theses and Dissertations

High temperature stress is among the most difficult to control abiotic factors affecting crop yields in the Southern United States due to its wide regional influence. Cotton (Gossypium hirsutum L.) though a tropical plant in origin, it is sensitive to the effects of high temperature. This is of particular concern when the warmest temperatures coincide with the most sensitive developmental stage of flowering. Thus, the capacity to improve cotton’s ability to tolerate heat stress has been a significant focus for many decades. Therefore, this research was composed of several different components all designed to investigate heat stress effects. Using a …

Effect Of Potassium Deficiency On Uptake And Partitioning In The Cotton (Gossypium Hirsutum L.) Plant And Detection By A Crop Reflectance Sensor, Taylor Dayne Coomer

Graduate Theses and Dissertations

For cotton (Gossypium hirsutum L.) to grow and develop normally, plants need to uptake the necessary amount of nutrients and use those nutrients in a beneficial fashion. It is recognized that cotton needs a certain tissue concentration of ions to achieve and maintain growth rates (Siddiqi et al., 1987). One of the most essential and abundant nutrients in cotton is potassium (K), second only by mass to nitrogen (N) (Marschner, 1995; Oosterhuis et al., 2013). Potassium exists in the soil in four separate pools and moves through soil to roots mainly through diffusion (Rengel & Damon, 2008; Samal et al., …

Investigation Of Parasympathetic Effects Of Lavender Essential Oil In Humans, Haakon Robert Nelson, Rachel Ann Burkhart, Daniel Morin, Nicole Grace Weissenfluh, Michael R. Korn

Montview Journal of Research & Scholarship

The purpose of this study will be to investigate the claim that administration of lavender (Lavandula angustifolia) essential oil (topically, orally, and/or respiratorily) produces a relaxative effect in human subjects. This investigation will theoretically be conducted in two stages. Stage one will focus primarily on determining the presence of therapeutic effects and the relative effectiveness of lavender in several application modalities. Stage two will proceed based on findings from stage one. If significant parasympathetic effects are observed in relation to one or more of the lavender oil modalities described above, a more focused investigation will be conducted in stage two …

Evaluating Pedestrial Compaction Variation & Soil Organic C Content, Gunner B. Decker

Scholars Week

Over time, playing fields are monitored by overall appearance. However, pedestrian compaction is not evenly distributed throughout the entire surface.

Seed Dispersal: A Tale Of Two Species, W. John Hayden

Biology Faculty Publications

Orchids have minute, dustlike seeds. In this respect, Goodyera pubescens (Downy Rattlesnake Plantain), the 2016 VNPS Wildflower of the Year, is a typical orchid. Like all other orchids, Goodyera seeds contain little more than a few embryonic cells enclosed in a thin seed coat. There are two advantages to small seed size in orchids: minute seeds can be produced in prodigious quantities, and they can disperse over great distances by wind.

Information Thermodynamics Of Cytosine Dna Methylation, Robersy Sanchez Rodriguez, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Cytosine DNA methylation (CDM) is a stable epigenetic modification to the genome and a widespread regulatory process in living organisms that involves multicomponent molecular machines. Genome-wide cytosine methylation patterning participates in the epigenetic reprogramming of a cell, suggesting that the biological information contained within methylation positions may be amenable to decoding. Adaptation to a new cellular or organismal environment also implies the potential for genome-wide redistribution of CDM changes that will ensure the stability of DNA molecules. This raises the question of whether or not we would be able to sort out the regulatory methylation signals from the CDM background …

Ws-2 Introgression In A Proportion Of Arabidopsis Thaliana Col-0 Stock Seed Produces Specific Phenotypes And Highlights The Importance Of Routine Genetic Verification, Mon-Ray Shao, Vikas Shedge, Hardik Kundariya, Fredric R. Lehle, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Arabidopsis thaliana is an important model organism with a robust network of resources that has been of enormous value to the plant science research community. The use of isogenic material as a reference point or control is critical for many types of experiments in plant molecular biology and genetics. Recently, we noticed that some seed from a common source of the widely used Columbia-0 (Col-0) strain gave rise to plants showing features atypical for this strain. Whole-genome DNA-sequencing and allelespecific PCR assays confirmed that the abnormal individuals contain multiple introgressions from the ecotype Wassilewskija-2 (Ws-2), as described below. This …

Student-Conducted Farmer Video Interviews, Jenn K. Simons, Justin Van Wart, Charles Francis, Kristyn Harms

Department of Agronomy and Horticulture: Faculty Publications

High school agricultural education teachers have expressed concern about the lack of easily accessible educational materials dealing with contemporary topics in sustainable agriculture. There are numerous textbooks and monographs available for farmers and students at the college level, including the highly practical resources available from the Sustainable Agriculture Research and Education (SARE) book series on soil fertility (Magdoff and van Es, 2010), cover crops (Bowman et al., 2007) and building a farm business (DiGiacomo et al., 2003), among others. Although these are full of color photos and easily accessible graphs and tables, they are still in the print media category. …

Effect Of Green Tea On Interaction Of Lipid Oxidation Products With Sarcoplasmic And Myofibrillar Protein Homogenates Extracted From Bovine Top Round Muscle, Nahathai Stapornkul, Tatiana Prytkova, Lilian Were

Food Science Faculty Articles and Research

The interaction between lipid oxidation products and bovine sarcoplasmic (SP) and myofibrillar protein (MP) homogenates in the presence of green tea was investigated. To monitor the effect of green tea on lipid oxidation, aldehydes were measured while effect on protein was monitored via changes in myoglobin, thiols, and tryptophan fluorescence over nine days of refrigerated storage. The presence of SP and MP decreased free aldehydes in the buffers. The SP bound more aldehydes than MP. The tea compounds exhibited more favorable binding energies than aldehydes near histidine 64 close to the heme moiety of myoglobin. Addition of tea lowered tryptophan …

Corn Era Hybrid Response To Nitrogen Fertilization, Krishna P. Woli, Matthew J. Boyer, Roger Wesley Elmore, John E. Sawyer, Lori J. Abendroth, Daniel W. Barker

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) N use is of continued interest due to agronomic performance and environmental issues. This 2-yr study evaluated era hybrid response to fertilizer nitrogen (FN) rate in a factorial arrangement of one popular hybrid per five decades (1960–2000 eras) and five N rates (0–224 kg N ha^–1). An additional hybrid per era was grown at 168 kg N ha^–1. Hybrid productivity and nitrogen use efficiency (NUE) increased across the eras, but not between the 1980 and 1990 eras. Grain yield (GY) increased 65% and total plant biomass 43%, however, total plant nitrogen …

Assessing How Disruption Of Methanogenic Communities And Their Syntrophic Relationships In Tidal Freshwater Marshes Via Saltwater Intrusion May Affect Ch4 Emissions, David J. Berrier, Scott C. Neubauer, Rima B. Franklin

Rice Rivers Center Research Symposium

Tidal freshwater wetlands (TFW), which lie at the interface of saltwater and freshwater ecosystems, are predicted to experience moderate salinity increases due to sea level rise. Increases in salinity generally suppress CH4 production, but it is uncertain to what extent elevated salinity will affect CH4 cycling in TFW. It is also unknown whether CH4 production will resume when freshwater conditions return. The ability to produce CH4 is limited to a monophyletic group of the Euryarchaeota phylum called methanogens (MG), who are limited to a small number of substrates (e.g., acetate, H2, and formate) produced from the breakdown of fermentation products. …

The Chemical Constituents Of New Essential Oils From Endemic And Sub Endemic Plants Of Mongolian Gobi, Sanduin Shatar, Jordanka Stavena, Milka Todorova, Shataryn Altantsetseg

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

The new essential oil-Endemic and sub endemic plants in Outer Mongolian Gobi were isolated by hydro distillation from the aerial parts of Brachanthemum gobicum Krasch, B. mongolicum Krach, B. mongolorum Grub., Asterothamnus centrali-asiaticus Novopork, A. molliusculus Novopork., Ajania trifida (Turcz) Tzvel, A. achilleoides (Turcz) Tzvel., Ferula ferulaeoides (Stend) Eug. Kor., Artemisia davajamczii Darijima., A. caespitosa Ldb, A. subchrysolepis Filat, A. schischkinii Krasch, A.intricata Franch, A.ordasica Krasch, A.gobica (Krasch) Grub., A. sphalerocephala Krasch that grown in wild in Outer Mongolian Gobi. The essential oils were analyzed by a combination of Capillary GC and GC-MS and their main constituents were described.

Mapping Qtls And Association Of Differentially Expressed Gene Transcripts For Multiple Agronomic Traits Under Different Nitrogen Levels In Sorghum, Malleswari Gelli, Sharon E. Mitchell, Kan Liu, Thomas E. Clemente, Donald P. Weeks, Chi Zhang, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Sorghum is an important C₄ crop which relies on applied Nitrogen fertilizers (N) for optimal yields, of which substantial amounts are lost into the atmosphere. Understanding the genetic variation of sorghum in response to limited nitrogen supply is important for elucidating the underlying genetic mechanisms of nitrogen utilization.

Results: A bi-parental mapping population consisting of 131 recombinant inbred lines (RILs) was used to map quantitative trait loci (QTLs) influencing different agronomic traits evaluated under normal N (100 kg.ha⁻¹ fertilizer) and low N (0 kg.ha⁻¹ fertilizer) conditions. A linkage map spanning 1614 cM was developed …

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such …

Simulating Long-Term Impacts Of Cover Crops And Climate Change On Crop Production And Environmental Outcomes In The Midwestern United States, Andrea D. Basche, Sotririos V. Archontoulis, Thomas C. Kaspar, Dan B. Jaynes, Timothy B. Parkin, Fernando E. Miguez

Department of Agronomy and Horticulture: Faculty Publications

It is critical to evaluate conservation practices that protect soil and water resources from climate change in the Midwestern United States, a region that produces one-quarter of the world’s soybeans and one-third of the world’s maize. An over-winter cover crop in a maize–soybean rotation offers multiple potential benefits that can reduce the impacts of higher temperatures and more variable rainfall; some of the anticipated changes for the Midwest. In this experiment we used the Agricultural Production Systems sIMulator (APSIM) to understand how winter rye cover crops impact crop production and environmental outcomes, given future climate change. We first tested APSIM …