Toxicology Commons^™

The Potential Role Of Phenolic Compounds On Modulating Gut Microbiota In Obesity, Gow-Chin Yen, Hsin-Lin Cheng, Li-Yu Lin, Shiuan-Chih Chen, Chin Lin Hsu

Journal of Food and Drug Analysis

Obesity is a rising public health issue and challenge which is tightly correlated with socio-economic development paralleled with increased energy intake and sedentary behavior that subsequently cause adipose tissue accumulation. Physiological and metabolic status changes during obesity development have been suggested with low grade inflammation of gastrointestinal tract. The gut microbiota plays an essential role in regulating whole body energy metabolism and also lipid accumulation, and immunity of host. However, the detail mechanism of which the gut microbiota composition influence obesity development in humans still need deeper investigation owing to the complex pathophysiology of such disease. Recently, the consumption of …

Toxicity Studies Of 1'-S-1'-Acetoxychavicol Acetate In Rat Model, Ali Abdalla Yasir Osman

Student Works (2020-2029)

Natural compounds are the main source of therapeutic molecules for various diseases in this era, and most of the current medicines are derived from plants, microorganisms or animals. Nevertheless, the safety profile of these natural compounds has to be confirmed prior to their medicinal use. 1'-S-1'-acetoxychavicol acetate (ACA), a type of phenylpropanoid extracted from Alpinia conchigera Griff., is one of the natural compounds being investigated for its potential therapeutic purposes. ACA was previously reported to reduce tumour volume in athymic mice at the effective dose of 1.56 mg/kg body weight intraperitoneally and induce apoptotic death of tumour cells through the …

Using Short Chain Fatty Acids To Inactivate Pseudomonas Aeruginosa Biofilms, Stephen Lee

Dissertations and Theses

Abstract

Using Short Chain Fatty Acids to Disinfect Pseudomonas Aeruginosa Biofilms

by

Stephen Lee

Background

The study of bacteria typically focuses on the planktonic or free-living single-cell state that is purely cultured in a laboratory for subsequent growth with appropriate media. Although this traditional way of growing bacteria has been paramount to the understanding of bacterial behavior, physiology, and pathogenesis, bacteria rarely exist as pure cultures of planktonic growth forms in the natural habitat. Bacterial contamination of environmental surfaces, in the form of biofilms, is a prime public health problem and causes numerous infections within the general public. Biofilms are …

Per- And Polyfluoroalkyl Substances In Consumable Fish & Surface Waters Near Sites Using Aqueous Film-Forming Foams: Potential Risks, Suggested Regulatory Standards And Policy Approaches For Massachusetts, Tachalla Gibeau

Sustainability and Social Justice

Per- and polyfluoroalkyl substances (PFAS) are emerging contaminants of concern with potential for adverse effects on both human and environmental health. Hence, long-chain PFAS compounds used in legacy aqueous film-forming foams (AFFFs) are being phased out, resulting in the stockpiling of them at various commercial installations. PFAS contaminated runoff from these facilities has the potential to contaminate nearby surface water bodies and the fish inhabiting them. An analytical approach was used to identify surface water bodies in Massachusetts that are habitats for fish susceptible to PFAS contamination and where said fish are also popularly consumed by locals – thereby representing …

Identification And Quantification Of Classic, Prescription, And Synthetic Opioids In Hair By Liquid Chromatography Tandem Mass Spectrometry (Lc/Ms/Ms), Natalia A. Platosz

Student Theses

The current use and misuse of synthetic and prescription opioids in the US has reached epidemic status. According to the US Department of Health and Human Services, every day more than 130 people in the US die after overdosing on opioids, and 2.1 million had an opioid use disorder in 2016. Hair is becoming an alternative matrix of increasing interest in forensic toxicology to investigate drug use and abuse patterns due to its long window of detection. The focus of this project was to develop and validate a method that simultaneously detects and quantifies 27 classic, prescription and synthetic opioids …

Analysis Of Cannabinoids In Serum By Gc-Ms/Ms, Christie Cannarozzi

Student Theses

Due to recent changes in federal and state legislations, the availability and consumption of cannabis products have increased in the United States. The expanded use of recreational and medicinal cannabis products increases the importance of implementing sensitive and selective instrumental methods in toxicological laboratories, as legal implications may arise in forensic cases, such as driving under the influence of drugs (DUID). The purpose of this study was to perform a cross-validation for the quantitative analysis of cannabinoids (Δ9-tetrahydrocannabinol, cannabidiol, cannabinol, 11-hydroxy-Δ9-tetrahydrocannabinol, and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol) in serum by gas chromatography coupled to triple quadrupole tandem mass spectrometry (GCTQ). This method was fully …

Dual Role Of Antimicrobial Peptide Cathelicidin In Alcohol-Associated Liver Disease., Fengyuan Li

Electronic Theses and Dissertations

Patients with alcohol-associated liver disease (ALD) have high morbidity and mortality in its severe forms. One of the major features in different forms of ALD is the altered gut microbiota. Antimicrobial peptides (AMPs) play a crucial role in maintaining gut microbiota homeostasis. LL-37 (known as CRAMP in mouse) is the sole member of the human cathelicidin family and has piqued great research interest for its dual role in modulating microbiota and the immune response in metabolic diseases. Inflammasome activation is an important component of the liver pathophysiology in ALD and requires two signals for its full activation to induce the …

Tobacco Product-Derived Aldehydes: Effects On Circulating Angiogenic Cells & Implications For Cardiovascular Disease., Jordan B. Lynch

Electronic Theses and Dissertations

Cardiovascular disease (CVD) is the leading cause of death worldwide. Tobacco smoke is the most significant modifiable risk factor in CVD development and contains numerous toxic compounds, including aldehydes, which have been linked to CVD. Formaldehyde, acetaldehyde, acrolein, and crotonaldehyde have been identified as significant contributors to cigarette-induced health effects, but the mechanism of these effects is not well understood. These aldehydes are also found in aerosols produced by e-cigarettes (e-cigs). The goal of this dissertation was to explore the systemic, hematological, and endothelium-related effects of exposure to tobacco products and constituent aldehydes and to identify potential mechanisms of injury. …

The Cardiovascular Effects Of Tobacco Product-Derived Aldehydes: Contribution Of Transient Receptor Potential Ankyrin-1., Lexiao Jin

Electronic Theses and Dissertations

Cardiovascular diseases (CVD) are the leading cause of death in most countries and are significant health and financial burden worldwide. Smoking contributes to more than 480,000 premature deaths annually -- accounting for one of every five deaths in the United States each year. Numerous studies have shown that smoking increases the risk of factors including endothelial dysfunction, thrombosis, and atherosclerosis, which may lead to an incidence of myocardial infarction, coronary artery disease, and stroke. Electronic cigarettes (e-cigarettes) are a new kind of tobacco product, and some studies show that e-cigarettes also could induce CVD since both e-cigarettes and conventional tobacco …

Tobacco-Derived Aldehydes: Platelet Activation, Thrombosis, And The Role Of Trpa1., Andre Dwayne Richardson

Electronic Theses and Dissertations

Cigarette smoking is the single largest risk factor for cardiovascular disease (CVD) pathophysiology. Numerous researchers have shown potential associations between aldehydes in tobacco-derived aerosols from mainstream cigarette smoke (MCS) or electronic cigarettes (e-cigs) and their cardiotoxicity by damaging blood vessel endothelium. The severity of exposure to these toxicants can furthermore lead to adverse cardiovascular outcomes such as myocardial infarctions, stroke, coronary and peripheral artery disease, or atherosclerosis due to blood clots, a pro-thrombotic event. However, the mechanisms by which levels of harmful and potentially harmful constituents (HPHCs) such as aldehydes induce thrombosis are not well-known. Of specific interest, evidence has …

Understanding The Toxicity Of Single Hydrocarbons, Oil, And Dispersed Oil: A Species Sensitivity Assessment For Five Atlantic Coral Species, Nicholas R. Turner

HCNSO Student Theses and Dissertations

Coral reefs are keystone coastal ecosystems that are at risk of exposure to petroleum hydrocarbons from a range of sources, including oil spill incidents and chronic runoff, and are usually one of the highest valued natural resources for protection in Net Environmental Benefit Analysis (NEBA)/Spill Impact Mitigation Assessment (SIMA) of response methods and environmental damage. Previous research evaluating hydrocarbon impacts to corals has resulted in no clear characterization of sensitivity, as work has generally focused on higher-level effects, compounded by significant variability in experimental methodology. This represents an important knowledge gap in oil spill preparedness and response as it relates …

The Influence Of Acidification And Copper Exposure On Copper Accumulation And Anti-Oxidant Enzyme Responses In The Pond Snail, Lymnaea Stagnalis, Gretchen K. Bielmyer-Fraser, Francis Alip, Ruth Adeyemi, Nolan Carney, Fasinia Santiago, Kyla Siemen, Kiley Donaghy

Georgia Journal of Science

Atmospheric carbon dioxide is rising at an accelerated rated due to increased anthropogenic activities. Metals have also been a noted problem; however, little research has addressed combined exposure of both pollutants to sensitive, calcifying organisms in freshwater habitats. This study examined copper accumulation (over 7 d) and activity of the antioxidant enzymes, catalase and glutathione peroxidase (over 2 d), in the freshwater common pond snail, Lymnaea stagnalis, after exposure to ambient and increased (2000 µatm) CO₂ and copper (control, 5, and 20 µg/L). Results demonstrated increased copper accumulation in soft tissue of snails exposed to copper; however, exposure to …

Assessment Of The Toxicological Effects Of Cnt-Ab In Mice Followed By Microwave Hyperthermia 14 Days Post Treatment., Andrew C. Mixson

Honors College Theses

Serious side effects and treatment resistance are the main impediments to successful cancer therapy. A variety of nanoparticles have been used for localized, site-specific treatment that prevent or circumvent these impediments. Microwaves alone have been previously used for thermal ablation of various tumors, setting precedence for their successful use in live organisms. In cell culture studies, it has been observed that multiwall carbon nanotubes (MWCNTs) instantly and efficiently absorb microwaves, causing hyperthermia of cells in direct contact with them with unnoticed harm to other cells. It is hypothesized that a treatment can be developed based on a safe microwave heating …

Investigating The Toxicology Of Intramuscular Injected Cnt-Ab In Mice Followed By Microwave Hyperthermia., Conner Clark

Honors College Theses

The advent of carbon nanotubes (CNTs) has led to a wide range of research in various fields including cancer therapy for targeting specific localized and site-specific treatment. Carbon nanotubes bound to tumor specific antibodies (Ab) offers specific treatment for cancer cells without affecting surrounding tissue. This treatment makes use of infrared absorptive properties of nanotubes to incinerate both the nanotube and its associated tumor in vivo. We seek to affirm the initial results of CNT in cancer therapy by investigating the toxicological effect in mice injected with CNT-Ab followed by microwave hypothermia. After 1-week post-injection, mice were sacrificed followed …

Association Of Long-Term Consumption Of Repeatedly Heated Mix Vegetable Oils In Different Doses And Hepatic Toxicity Through Fat Accumulation, Gul Ambreen, Afshan Siddiq, Kashif Hussain

Department of Biological & Biomedical Sciences

Background: Hepatic diseases are one of the chief reasons for worldwide morbidity and mortality. The increased incidence in Asian countries is driving researchers to explore preventive ways from nature. It is more practical to go with healthy routine edibles like vegetable oils to avoid environmental and chemical hepatic injuries. With the use of thermally oxidized oils overproduction of reactive oxygen species (ROS) with overwhelmed cellular antioxidants defense system results in oxidative stress, the known cause of cardiovascular diseases (CVDs), cancers and neurodegenerative disorders. Little is investigated about the effect of daily used oxidized cooking oils on hepatic function changes with …

An Evaluation Of The Susceptibility Of Goats To Larkspur Toxicosis, Kevin D. Welch, Clint A. Stonecipher, Dale R. Gardner, Benedict T. Green, Daniel Cook

Poisonous Plant Research (PPR)

Larkspurs (Delphinium spp.) are a major cause of cattle losses in western North America, whereas sheep have been shown to be resistant to larkspur toxicosis. Goats are often used as a small ruminant model to study poisonous plants, even though they can be more resistant to some poisonous plants. It is not known how susceptible goats are to the adverse effects of larkspurs. In this study, we evaluated the susceptibility of goats to larkspur toxicosis by performing a dose-response study. We dosed goats with D. barbeyi collected near Cedar City, Utah at 3.3, 4.4, 6.6, 8.8 and 10.0 g …

Assessing Field-Scale Risks Of Foliar Insecticide Applications To Monarch Butterfly (Danaus Plexippus) Larvae, Niranjana Krishnan, Yang Zhang, Keith G. Bidne, Richard L. Hellmich, Joel R. Coats, Steven P. Bradbury

Biological Sciences Faculty Research & Creative Works

Establishment and maintenance of milkweed plants (Asclepias spp.) in agricultural landscapes of the north central United States are needed to reverse the decline of North America's eastern monarch butterfly (Danaus plexippus) population. Because of a lack of toxicity data, it is unclear how insecticide use may reduce monarch productivity when milkweed habitat is placed near maize and soybean fields. To assess the potential effects of foliar insecticides, acute cuticular and dietary toxicity of 5 representative active ingredients were determined: beta-cyfluthrin (pyrethroid), chlorantraniliprole (anthranilic diamide), chlorpyrifos (organophosphate), and imidacloprid and thiamethoxam (neonicotinoids). Cuticular median lethal dose values for …

The Impact Of Nicotine Accumulation Exposure On Lithobates Catebeianus Larvae Mortality, Luke Micek

UCARE: Research Products

Cigarette butts are the most littered item in the world and contain over 4,000 chemicals, including the organic compound nicotine (Slaughter et al. 2011). Billions of cigarette butts are littered each year which may wash into bodies of water, impacting the local wildlife. To determine how introduced chemicals impact the environment, it is important to study its effects on indicator species. Amphibians, such as Lithobates catebeianus, act as indicator species due to their extreme sensitivity to chemical changes in its environment. The purpose of this research project was to obtain data to help determine the impact nicotine accumulation has …

Multi-Label Model For Toxicity Prediction, Xiu Huan Yap, Michael L. Raymer

Celebration of Undergraduate & Graduate Research, Scholarship, and Creative Activities Materials

Most computational predictive models are specifically trained for a single toxicity endpoint. Since more than 1300 toxicity assays have been reported in the TOXCAST dashboard, achieving high coverage over this growing number of toxicity endpoints remains challenging. Furthermore, single-endpoint models lack the ability to learn dependencies between endpoints, such as those targeting similar biological pathways, which may be used to boost model performance. In this study, we characterize the performance of 3 multi-label classification (MLC) models, namely Classifier Chains (CC), Label Powersets (LP) and Stacking (SBR), on Tox21 challenge data. These MLC models employ the Problem Transformation approach, which is …

Herbicide Injury From Dicamba And 2,4-D: How Much Is Too Much In Lettuce?, Xinzheng Chen, Amit J. Jhala, Stevan Z. Knezevic, Samuel E. Wortman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Off-target herbicide injury from dicamba and 2,4-D is an increasingly common problem for specialty crop growers in the Midwest United States. Lettuce (Lactuca sativa L.) is a common specialty crops grown in Nebraska, but proximity to corn and soybean production leaves growers vulnerable to crop injury and significant economic loss. The goal of this study was to quantify crop injury and yield loss in greenhouse grown lettuce after exposure to simulated sub-lethal drift rates of 2,4-D and dicamba. Sublethal doses were determined based on a percentage of the maximum labeled rate and ranged from 25% to 0.01%. Tested lettuce …

The Effects Of Trichloroethylene On The Germination And Early Development Of Radish, Butternut Squash, Corn, And Soybean, Sarah Jaksich

UNO Student Research and Creative Activity Fair

Trichloroethylene (TCE) is a small molecule used as a metal degreaser and as an extraction solvent for lipids. Historically, TCE has been used to clean missiles and rockets after testing at military sites. However, TCE has decreased in use due to its potential health hazards. TCE is particularly dangerous because it is soluble in water and easily seeps into ground water. As a result, TCE has contaminated groundwater in Mead, Nebraska. The town is near the formal Nebraska Ordnance Plant, a military factory specializing in bomb assembly, ammonium nitrate production, and explosives burning. Soil sampling in Mead indicated TCE levels …

Data From: Consumption Of The Total Western Diet Promotes Colitis And Inflammation-Associated Colorectal Cancer In Mice, Abby D. Benninghoff

Browse all Datasets

Gene expression data for colon tissues obtained prior to, during, and after onset of colitis in mice fed either a standard or Western type diet.

File 1: probe annotations.

File 2: nSolver differential expression analysis results.

File 3: Gene set analysis

File 4: nSolver pathway scores

File 5: STRING-db networks and clusters

File 6: Metascape ontology results

File 7: nSolver cell type profiling scores

Investigation On The Potential Of Dried Blood Stain For The Detection Of Amphetamine Type Stimulants By Enhanced Methods Of Liquid Chromatography Tandem-Mass Spectrometry, Ahmad Zubaidi Fathiah

Student Works (2020-2029)

The investigation on the potential of dried blood stain for Amphetamine Type Stimulants (ATS) has been divided into four major parts, from which independent conclusions are drawn. The first part is the development and validation of a novel method for the simultaneous determination of the drugs using liquid chromatography with electrospray ionization tandem mass spectrometer (LC-ESI-MS/MS). The method is enhanced from normal Multi-Reaction Mode (MRM) to Multi-Reaction Mode-Enhanced Product Ion Mode- Multi-Reaction Mode 3 (MRM-EPI-MRM3) and tested in whole blood specimens (WBS), dried blood stain (DBS) and urine specimens as well as applied to real forensic cases in Malaysia. The …

Impacts Of The Deepwater Horizon Oil Spill On Reproduction And Ontogeny Of Gulf Killifish (Fundulus Grandis), Charles Alexander Brown

LSU Doctoral Dissertations

On April 20, 2010, the Deepwater Horizon oil spill released an estimated 779 million liters of Macondo-252 crude oil into the Gulf of Mexico, making it the largest marine oil spill in history. Over a thousand kilometers of marshland that many species of fish use as a spawning grounds and nurseries was oiled, exposing breeding adult fish and their offspring to oil. My dissertation investigates the impacts of the Deepwater Horizon oil spill and associated remediation efforts on the reproduction and ontogeny of a sentinel ecotoxicological species, the Gulf killifish (Fundulus grandis). Concerns were raised regarding use of …

Natural Sources And Bioactivities Of 2,4-Di-Tert-Butylphenol And Its Analogs, Fuqiang Zhao, Ping Wang, Rima D. Lucardi, Zushang Su, Shiyou Li

NCPC Publications and Patents

2,4-Di-tert-butylphenol or 2,4-bis(1,1-dimethylethyl)-phenol (2,4-DTBP) is a common toxic secondary metabolite produced by various groups of organisms. The biosources and bioactivities of 2,4-DTBP have been well investigated, but the phenol has not been systematically reviewed. This article provides a comprehensive review of 2,4-DTBP and its analogs with emphasis on natural sources and bioactivities. 2,4-DTBP has been found in at least 169 species of bacteria (16 species, 10 families), fungi (11 species, eight families), diatom (one species, one family), liverwort (one species, one family), pteridiphyta (two species, two families), gymnosperms (four species, one family), dicots (107 species, 58 families), monocots (22 species, …

Mechanisms Of Dopamine-Induced Methamphetamine Neurotoxicity, Melinda L. Asbury

Theses, Dissertations and Capstones

Methamphetamine (MA) neurotoxicity is particularly evident in the striatum where it causes extensive dopamine (DA) release and results in neurodegeneration. To identify specific signaling pathways and macromolecules involved in postsynaptic DA-induced striatal toxicity we used a SK-N-MC cell model that mimics postsynaptic D1 receptor-expressing striatal neurons. The cells were treated for 6-24 h with 0-50 µM DA. The concentration was chosen to impart physiological relevance to the study as it mirrors [DA] found within the striatum following MA exposure. We show that 25-50 µM DA resulted in protein changes consistent with nitro(oxidative) stress as well as enhanced cleavage of caspase …

Adaptive Tolerance To Sodium Chloride In Daphnia Magna, Brady P. Parlato, Rick Kopp

Kentucky Journal of Undergraduate Scholarship

Salinity levels of some freshwater systems have been altered by humans, posing a threat to aquatic life. We hypothesized that a Daphnia magna population pre-treated with a low concentration of NaCl would develop greater salinity tolerance than an untreated population. Two Daphnia magna cultures with 60 individuals each were established, one started with 1.00 ppt NaCl and increased by 0.20 ppt weekly, and the other with pure spring water. After 4 weeks, 40 neonates from each culture were individually placed in a solution of 2.30 ppt NaCl (separately determined to be the LC₅₀) for 48 hours. Survival was …

Novel Post-Translational Modification And Function Of Fus: The Relevance To Amyotrophic Lateral Sclerosis, Alexandra Arenas

Theses and Dissertations--Toxicology and Cancer Biology

Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease characterized by the preferential death of motor neurons. Approximately 10% of ALS cases are familial and 90% are sporadic. Fused in Sarcoma (FUS) is a ubiquitously expressed RNA binding protein implicated in familial ALS and frontotemporal dementia (FTD). FUS is ubiquitously expressed in cells and has a variety of functions in the nucleus and cytoplasm. FUS mutations in the nuclear localization sequence (NLS) causes mislocalization of FUS in the cytoplasm, where it can undergo liquid-liquid phase separation and become stress granules or protein inclusions. Although FUS inclusion bodies can be found in …

Solvants Verts : Fondements, Santé, Sécurité, Environnement Et Substitution, Denis Bégin, Caroline Couture, Michel Gérin, Maximilien Debia

Rapports de recherche scientifique

L’utilisation de solvants dans de nombreuses applications (dégraissage, nettoyage, décapage, imprimerie, peintures, adhésifs, fabrication de produits pharmaceutiques) constitue une préoccupation majeure. En effet, un grand nombre d’entre eux sont associés à des effets nocifs, tant sur la santé et la sécurité des travailleurs (brûlure, cancer, dermatite, encéphalopathie, fœtotoxicité, polynévrite) que sur l’environnement et la santé de la population générale (précurseurs de smog, pollution des eaux, couche d’ozone). Par ailleurs, de plus en plus d’entreprises veulent prendre le virage vert, c.-à-d. faire en sorte que leurs activités et leurs produits s’inscrivent dans la démarche du développement durable. C’est dans ce contexte …

The Effect Of Seasonality On Yellow Perch Ecology And Ecotoxicology Within Lake Manganese, Bailey Duxbury

Dissertations, Master's Theses and Master's Reports

Seasonality is a consistent component of aquatic ecosystems yet most fish biological and ecotoxicological studies commonly employ field sampling protocols focused during the warm open water season with minimal emphasis placed on winter sampling, especially for north-temperate latitude ecosystems. Such strategies limit our understanding of poikilotherm biology and ecology during the overwintering seasons. Here, I investigated seasonal changes in yellow perch (Perca flavescens) biology, ecology and ecotoxicology over a one-year period in Lake Manganese. Significant seasonality was observed for metrics including fish energy densities (kJ/g), gonadosomatic indices, whole-body lipid contents, and carbon stable isotope values (δ¹³C). …