Open Access. Powered by Scholars. Published by Universities.®

Pharmacology Commons™

Open Access. Powered by Scholars. Published by Universities.®

Toxicology

Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 991 - 1020 of 1180

Full-Text Articles in Pharmacology

Analytical Methods For Cholesterol Quantification, L.-H. Li, E.P. Dutkiewicz, Y.-C. Huang, H.-B. Zhou, C.-C. Hsu Jul 2020

Analytical Methods For Cholesterol Quantification, L.-H. Li, E.P. Dutkiewicz, Y.-C. Huang, H.-B. Zhou, C.-C. Hsu

Journal of Food and Drug Analysis

Cholesterol is an important lipid molecule in cell membranes and lipoproteins. Cholesterol is also a precursors of steroid hormones, bile acids, and vitamin D. Abnormal levels of cholesterol or its precursors have been observed in various human diseases, such as heart diseases, stroke, type II diabetes, brain diseases and many others. Therefore, accurate quantification of cholesterol is important for individuals who are at increased risk for these diseases. Multiple analytical methods have been developed for analysis of cholesterol, including classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS). Strategy known as ambient ionization mass …

Go to article

Identification Of Bacteria In Juice/Lettuce Using Magnetic Nanoparticles And Selected Reaction Monitoring Mass Spectrometry, C.-T. Chen, J.-W. Yu, Y.-P. Ho Jul 2020

Identification Of Bacteria In Juice/Lettuce Using Magnetic Nanoparticles And Selected Reaction Monitoring Mass Spectrometry, C.-T. Chen, J.-W. Yu, Y.-P. Ho

Journal of Food and Drug Analysis

Ensuring food safety requires a rapid and reliable method for detecting food-borne pathogens. Mass spectrometry has been demonstrated as a powerful tool to classify pure bacterial species. However, matrix interference from food backgrounds may lead to false results because of the suppression of microbial signals. It is useful to develop a method for bacterial enrichment and marker identification in food samples. Magnetic zirconia nanoparticles were used to concentrate spiked microorganisms from apple juice/lettuce under specific conditions (pH 4.5). Bacterial identification was achieved using nanoLC–MS. Selected reaction monitoring of bacteria-related peptides was applied for the first time to identify bacteria including …

Go to article

Stability Of Glyoxal- And Methylglyoxal-Modified Hemoglobin On Dried Blood Spot Cards As Analyzed By Nanoflow Liquid Chromatography Tandem Mass Spectrometry, H.-J.C. Chen, Y.-C. Teng Jul 2020

Stability Of Glyoxal- And Methylglyoxal-Modified Hemoglobin On Dried Blood Spot Cards As Analyzed By Nanoflow Liquid Chromatography Tandem Mass Spectrometry, H.-J.C. Chen, Y.-C. Teng

Journal of Food and Drug Analysis

Blood sampling by the dried blood spot (DBS) technique has become commonly applied in newborn screening. It is often used for analysis of small molecules, such as metabolites. Recently, DBS sampling has been applied for quantification of post-translational protein modifications. Glyoxal and methylglyoxal are two simple oxoaldehydes released from glycated proteins in the Maillard reaction. They are widely distributed in the environment (e.g. cigarette smoke) and found in foods and beverages. Glyoxal and methylglyoxal are shown to react with biomolecules including DNA and proteins. In this laboratory, we previously identified the sites of modification by these two oxoaldehydes in human …

Go to article

Method Development Of Immunoglobulin G Purification From Micro-Volumes Of Human Serum For Untargeted And Targeted Proteomics-Based Antibody Repertoire Studies, Y.-T. Chang, M.-C. Chang, Y.-J. Tsai, C. Ferng, H.-C. Shih, Y.-P. Kuo, C.-H. Chen, I.-L. Tsai Jul 2020

Method Development Of Immunoglobulin G Purification From Micro-Volumes Of Human Serum For Untargeted And Targeted Proteomics-Based Antibody Repertoire Studies, Y.-T. Chang, M.-C. Chang, Y.-J. Tsai, C. Ferng, H.-C. Shih, Y.-P. Kuo, C.-H. Chen, I.-L. Tsai

Journal of Food and Drug Analysis

Immunoglobulins (Igs) are major serum proteins which play important roles in immunity. Both untargeted and targeted proteomic workflows can be applied to investigate antigen-binding sites and the glycosylation profiles of Igs. For a more-comprehensive picture of IgG from human serum, we developed an IgG purification process and coupled the standardized method to untargeted and targeted proteomic workflows for IgG investigations. Parameters such as the type of purification beads, volume of the bead slurry, incubation conditions, and binding capacities were evaluated in this study. Only 2 μL of human serum was required for each sample. The performance of coupling the purification …

Go to article

Pon-1 Carbamylation Is Enhanced In Hdl Of Uremia Patients, C.-T. Chang, Y.-P. Lim, C.-W. Lee, H.-Y. Liao, F.-Y. Chen, C.-M. Chang, F.-Y. Tang, C.-Y. Yang, C.-J. Chen Jul 2020

Pon-1 Carbamylation Is Enhanced In Hdl Of Uremia Patients, C.-T. Chang, Y.-P. Lim, C.-W. Lee, H.-Y. Liao, F.-Y. Chen, C.-M. Chang, F.-Y. Tang, C.-Y. Yang, C.-J. Chen

Journal of Food and Drug Analysis

High-density lipoprotein (HDL) carbamylation has been known in uremia patients. Paraoxonase-1 (PON-1) is an important HDL protein responsible for HDL anti-oxidant, arylesterase and lactonase activities. PON-1 carbamylation in uremic HDL has never been explored. We isolated HDL from uremia patients and control healthy subjects for study. Sandwich ELISA was used to estimate carbamylated PON-1 protein expression in HDL, and nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) was applied to identify the amino acid in PON-1 carbamylated. PON-1 enzyme activities were estimated by substrates conversion method. HDL anti-oxidant activity was gauged by fluorescence changes of indicator dye in the presence of H …

Go to article

Analysis Of Heterocyclic Amines In Meat Products By Liquid Chromatography – Tandem Mass Spectrometry, S.-H. Jian, P.-J. Yeh, C.-H. Wang, H.-C. Chen, S.-F. Chen Jul 2020

Analysis Of Heterocyclic Amines In Meat Products By Liquid Chromatography – Tandem Mass Spectrometry, S.-H. Jian, P.-J. Yeh, C.-H. Wang, H.-C. Chen, S.-F. Chen

Journal of Food and Drug Analysis

Heterocyclic amines (HCAs), a class made up of more than 25 compounds, are unintended hazardous substances that are generated by the heating or processing of proteinaceous foods at high temperatures. The International Agency for Research on Cancer (IARC) has classified four such HCAs (IQ, MeIQ, MeIQx, and PhIP) as being probable or possible human carcinogens. In this study, two sample preparation strategies, liquid–liquid extraction (LLE) with solid-phase extraction (SPE) and a rapid, easy, cheap, effective, rugged, and safe extraction (QuEChERS) method, were investigated for the determination of 11 types of HCAs in meat products by LC-MS/MS. The HCAs in the …

Go to article

Development Of Biomarkers Of Genitourinary Cancer Using Mass Spectrometry-Based Clinical Proteomics, Y.-T. Chen, C.-H. Tsai, C.-L. Chen, J.-S. Yu, Y.-H. Chang Jul 2020

Development Of Biomarkers Of Genitourinary Cancer Using Mass Spectrometry-Based Clinical Proteomics, Y.-T. Chen, C.-H. Tsai, C.-L. Chen, J.-S. Yu, Y.-H. Chang

Journal of Food and Drug Analysis

Prostate, bladder and kidney cancer are the three most common types of genitourinary cancer in the world. Of these, prostate and bladder cancers are within the top 10 most common cancers in men. Notably, kidney cancer causes no obvious symptoms in the early stages. To satisfy clinical-management requirements, researchers have developed numerous biomarkers by applying proteomic approaches using clinical serum, urine and tissue specimens, as well as cell and animal models. Through application of biomarker pipeline protocols, including discovery, verification and validation phases, and mass-spectrometric based proteomic platforms coupled with multiplexed quantification assays, these studies have led to recent rapid …

Go to article

Using Ambient Mass Spectrometry And Lc–Ms/Ms For The Rapid Detection And Identification Of Multiple Illicit Street Drugs, C.-H. Chiang, H.-H. Lee, B.-H. Chen, Y.-C. Lin, Y.-Y. Chao, Y.-L. Huang Jul 2020

Using Ambient Mass Spectrometry And Lc–Ms/Ms For The Rapid Detection And Identification Of Multiple Illicit Street Drugs, C.-H. Chiang, H.-H. Lee, B.-H. Chen, Y.-C. Lin, Y.-Y. Chao, Y.-L. Huang

Journal of Food and Drug Analysis

In this study the recently developed technique of thermal desorption electrospray ionization/mass spectrometry (TD–ESI/MS) was applied to the rapid analysis of multiple controlled substances. With the reallocation of mass spectral resources [from a standard ESI source coupled with liquid chromatography (LC) to an ambient TD–ESI source], this direct-analysis technique allows the identification of a wider range of illicit drugs through a dual-working mode (pretreatment-free qualitative screening/conventional quantitative confirmation). Through 60-MRM (multiple reaction monitoring) analysis—in which the MS/MS process was programmed to sequentially scan 60 precursor ion/product ion transitions and, thereby, identify 30 compounds (two precursor/product ion transitions per compound)—of a …

Go to article

Measuring Serum Total And Free Indoxyl Sulfate And P-Cresyl Sulfate In Chronic Kidney Disease Using Uplc-Ms/Ms, C.-N. Lin, I.-W. Wu, Y.-F. Huang, S.-Y. Peng, Y.-C. Huang, H.-C. Ning Jul 2020

Measuring Serum Total And Free Indoxyl Sulfate And P-Cresyl Sulfate In Chronic Kidney Disease Using Uplc-Ms/Ms, C.-N. Lin, I.-W. Wu, Y.-F. Huang, S.-Y. Peng, Y.-C. Huang, H.-C. Ning

Journal of Food and Drug Analysis

Chronic kidney disease (CKD) is a complex disorder that affects multiple organs and increases the risk of cardiovascular complications. CKD affects approximately 12% of the population in Taiwan. Loss of kidney function leads to accumulation of potentially toxic compounds such as indoxyl sulfate (IS) and p-cresyl sulfate (pCS), two protein-bound uremic solutes that can stimulate the progression of CKD. The aim of this study was to assess whether IS and pCS levels were correlated with CKD stage. We developed and validated a method for quantitating total and free IS and pCS in serum by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). …

Go to article

Exposure Marker Discovery Of Di-2(Propylheptyl) Phthalate Using Ultra-Performance Liquid Chromatography-Mass Spectrometry And A Rat Model, C.-L. Shih, J.-Y. Hsu, C.-P. Tien, Y.-N. Chung, V.G. Zgoda, P.-C. Liao Jul 2020

Exposure Marker Discovery Of Di-2(Propylheptyl) Phthalate Using Ultra-Performance Liquid Chromatography-Mass Spectrometry And A Rat Model, C.-L. Shih, J.-Y. Hsu, C.-P. Tien, Y.-N. Chung, V.G. Zgoda, P.-C. Liao

Journal of Food and Drug Analysis

Di-(2-propylheptyl) phthalate (DPHP) is a plasticizer and has been suggested to be a subchronic toxicant in rats. DPHP has been approved to be used in food containers and handling by the U.S. Food and Drug Administration. The use of DPHP is still increasing, and the risk of human exposure to DPHP via food may be high. Exposure markers measured in human samples are commonly used to monitor human exposure levels. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and a rat model were used to discover tentative DPHP exposure markers. DPHP and mono-(2-propylheptyl) phthalate (MPHP) were used as the precursors for calculating metabolite …

Go to article

Chemical Isotope Labeling Liquid Chromatography Mass Spectrometry For Investigating Acute Dietary Effects Of Cow Milk Consumption On Human Urine Metabolome, D. Mung, L. Li Jul 2020

Chemical Isotope Labeling Liquid Chromatography Mass Spectrometry For Investigating Acute Dietary Effects Of Cow Milk Consumption On Human Urine Metabolome, D. Mung, L. Li

Journal of Food and Drug Analysis

Biomarker discovery has been increasingly important in the field of metabolomics for the detection and understanding of diseases. Of the many biofluids available for metabolomics, urine is a preferred option as it is non-invasive to collect and contains a wide range of metabolites reflective of the health status of the testing individual. However, urine also contains many exogenous metabolites which are introduced through various sources such as diet. This complicates the data interpretation when searching the metabolome for disease-related endogenous metabolites. Since diet is difficult to control, this work aims to study the acute effects of diet (particularly cow milk) …

Go to article

Quantitation Of Serum 25(Oh)D2 And 25(Oh)D3 Concentrations By Liquid Chromatography Tandem Mass Spectrometry In Patients With Diabetes Mellitus, Y.-C. Lin, H.-H. Lee, S.-C. Tseng, K.-D. Lin, L.-P. Tseng, J.-F. Lee, Y.-H. Lee, B.-H. Chen Jul 2020

Quantitation Of Serum 25(Oh)D2 And 25(Oh)D3 Concentrations By Liquid Chromatography Tandem Mass Spectrometry In Patients With Diabetes Mellitus, Y.-C. Lin, H.-H. Lee, S.-C. Tseng, K.-D. Lin, L.-P. Tseng, J.-F. Lee, Y.-H. Lee, B.-H. Chen

Journal of Food and Drug Analysis

Vitamin D has been considered to regulate calcium and phosphorus homeostasis and to preserve skeletal integrity. Serum 25-hydroxyvitamin D (25(OH)D) is the best indicator of vitamin D levels. The association of serum 25(OH)D deficiency with increased risk of type 1 diabetes (T1DM) and type 2 diabetes (T2DM) is controversial. We investigated serum 25(OH)D 2 and 25(OH)D 3 levels in diabetes patients by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Serum 25(OH)D2 and 25(OH)D3 levels were measured with liquid chromatography tandem mass spectrometry in electrospray ionization positive mode. Chromatograms were separated using an ACE5 C18 column on a gradient of methanol. …

Go to article

Rapid Identification Of Herbal Toxins Using Electrospray Laser Desorption Ionization Mass Spectrometry For Emergency Care, H. Su, K.-T. Liu, B.-H. Chen, Y.-P. Lin, Y.-M. Jiang, Y.-H. Tsai, F.-R. Chang, J. Shiea, C.-W. Lee Jul 2020

Rapid Identification Of Herbal Toxins Using Electrospray Laser Desorption Ionization Mass Spectrometry For Emergency Care, H. Su, K.-T. Liu, B.-H. Chen, Y.-P. Lin, Y.-M. Jiang, Y.-H. Tsai, F.-R. Chang, J. Shiea, C.-W. Lee

Journal of Food and Drug Analysis

The unintentional ingestion of toxic compounds in herbs is not uncommon in many parts of the world. To provide timely and life-saving care in the emergency department, it is essential to develop a point-of-care analytical method that can rapidly identify these toxins in herbs. Since electrospray laser desorption ionization mass spectrometry (ELDI/MS) has been successfully used to characterize non-volatile chemical compounds without sample preparation, it was used to identify toxic herbal compounds in this study. The herbal toxins were collected either by sweeping a metallic probe across the surface of a freshly cut herb section or by directly sampling extracts …

Go to article

Complete Mapping Of Disulfide Linkages For Etanercept Products By Multi-Enzyme Digestion Coupled With Lc-Ms/Ms Using Multi-Fragmentations Including Cid And Etd, L.-J. Huang, C.-W. Chiang, S.-L. Chen, S.-Y. Wei, S.-H. Chen Jul 2020

Complete Mapping Of Disulfide Linkages For Etanercept Products By Multi-Enzyme Digestion Coupled With Lc-Ms/Ms Using Multi-Fragmentations Including Cid And Etd, L.-J. Huang, C.-W. Chiang, S.-L. Chen, S.-Y. Wei, S.-H. Chen

Journal of Food and Drug Analysis

The disulfide linkages of two etanercept products, Enbrel® (innovator drug) and TuNEX®, were characterized and compared using a multi-fragmentation approach consisting of electron transfer dissociation (ETD) and collision induced dissociation (CID) in combination with multi-enzyme digestion protocols (from Lys-C, trypsin, Glu-C, and PNGase F). Multi-fragmentation approach allowed multi-disulfide linkages contained in a peptide to be un-ambiguously assigned based on the cleavage of both the disulfide and the backbone linkages in a MS 3 schedule. New insights gained using this approach were discussed. A total of 29 disulfides, Cys18-Cys31, Cys32-Cys45, Cys35-Cys53, Cys56-Cys71, Cys74-Cys88, Cys78-Cys-96, Cys98-Cys104, Cys112-Cys121, Cys115-Cys139, Cys-142-Cys157, Cys163-Cys178 in TNFR …

Go to article

Targeting Amine- And Phenol-Containing Metabolites In Urine By Dansylation Isotope Labeling And Liquid Chromatography Mass Spectrometry For Evaluation Of Bladder Cancer Biomarkers, Y.-T. Chen, H.-C. Huang, Y.-J. Hsieh, S.-H. Fu, L. Li, C.-L. Chen, L.J. Chu, J.-S. Yu Jul 2020

Targeting Amine- And Phenol-Containing Metabolites In Urine By Dansylation Isotope Labeling And Liquid Chromatography Mass Spectrometry For Evaluation Of Bladder Cancer Biomarkers, Y.-T. Chen, H.-C. Huang, Y.-J. Hsieh, S.-H. Fu, L. Li, C.-L. Chen, L.J. Chu, J.-S. Yu

Journal of Food and Drug Analysis

Metabolomics is considered an effective approach for understanding metabolic responses in complex biological systems. Accordingly, it has attracted increasing attention for biomarker discovery, especially in cancer. In this study, we used a non-invasive method to evaluate four urine metabolite biomarker candidates—o-phosphoethanolamine, 3-amio-2-piperidone, uridine and 5-hydroxyindoleactic acid—for their potential as bladder cancer diagnostic biomarkers. To analyze these targeted amine- and phenol-containing metabolites, we used differential 12 C 2 -/ 13 C 2 -dansylation labeling coupled with liquid chromatography/tandem mass spectrometry, which has previously been demonstrated to exhibit high sensitivity and reproducibility. Specifically, we used ultra-performance liquid chromatography (UPLC) coupled with high-resolution …

Go to article

Glycoproteomic Identification Of Novel Plasma Biomarkers For Oral Cancer, S.-C. Chang, W.-L. Lin, Y.-F. Chang, C.-T. Lee, J.-S. Wu, P.-H. Hsu, C.-F. Chang Jul 2020

Glycoproteomic Identification Of Novel Plasma Biomarkers For Oral Cancer, S.-C. Chang, W.-L. Lin, Y.-F. Chang, C.-T. Lee, J.-S. Wu, P.-H. Hsu, C.-F. Chang

Journal of Food and Drug Analysis

Oral cancer with high incidence rates is occurring in many countries including in India, Pakistan, Bangladesh, Sri Lanka and Taiwan. Smoking, alcoholism, and betel nut chewing are considered to be the main risk factors for oral cancer. Further, deaths from oral cancer have increased year by year. Although several oral cancer-associated biomarkers have been reported, very few useful biomarkers have been applied for early diagnosis. Therefore, the investigation of oral cancer-specific biomarkers is urgently needed. We previously investigated N-glycomes of oral cancer cells and patient plasma. We found that both mRNA levels of FUT8 and core-fucosylated glycoproteins increase in cases …

Go to article

Reduction Of Aluminum Ion Neurotoxicity Through A Small Peptide Application – Nap Treatment Of Alzheimer's Disease, M.-H. Yang, S.-C. Chen, Y.-F. Lin, Y.-C. Lee, M.-Y. Huang, K.-C. Chen, H.-Y. Wu, P.-C. Lin, I. Gozes, Y.-C. Tyan Jul 2020

Reduction Of Aluminum Ion Neurotoxicity Through A Small Peptide Application – Nap Treatment Of Alzheimer's Disease, M.-H. Yang, S.-C. Chen, Y.-F. Lin, Y.-C. Lee, M.-Y. Huang, K.-C. Chen, H.-Y. Wu, P.-C. Lin, I. Gozes, Y.-C. Tyan

Journal of Food and Drug Analysis

Alzheimer's disease (AD) is the most common cause of dementia in late life. It is difficult to precisely diagnose AD at early stages, making biomarker search essential for further developments. The objective of this study was to identify protein biomarkers associated with aluminum ions toxicity (AD-like toxicity) in a human neuroblastoma cell model, SH-SY5Y and assess potential prevention by NAP (NAPVSIPQ). Complete proteomic techniques were implemented. Four proteins were identified as up-regulated with aluminum ion treatment, CBP80/20-dependent translation initiation factor (CTIF), Early endosome antigen 1 (EEA1), Leucine-rich repeat neuronal protein 4 (LRRN4) and Phosphatidylinositol 3-kinase regulatory subunit beta (PI3KR2). Of …

Go to article

Direct And Rapid Characterization Of Illicit Drugs In Adulterated Samples Using Thermal Desorption Electrospray Ionization Mass Spectrometry, S.-C. Cheng, Y.-D. Tsai, C.-W. Lee, B.-H. Chen, J. Shiea Jul 2020

Direct And Rapid Characterization Of Illicit Drugs In Adulterated Samples Using Thermal Desorption Electrospray Ionization Mass Spectrometry, S.-C. Cheng, Y.-D. Tsai, C.-W. Lee, B.-H. Chen, J. Shiea

Journal of Food and Drug Analysis

Foods and drinks have been adulterated with illicit drugs to facilitate criminal activities. Unfortunately, conventional analytical methods are incapable of rapidly characterizing these drugs in samples, as serious interferences from sample matrices must be removed through tedious and time-consuming pretreatment. Ambient ionization mass spectrometry (AMS) generally does not require sample pretreatment and is thus a suitable tool for directly and rapidly detecting illicit drugs in samples in different physical states. In this study, thermal desorption electrospray ionization mass spectrometry (TD-ESI/MS), an AMS technique, was utilized to efficiently characterize illicit drugs spiked in samples including drinks, powders, and jelly candies. To …

Go to article

Using Gas Chromatography And Mass Spectrometry To Determine 25-Hydroxyvitamin D Levels For Clinical Assessment Of Vitamin D Deficiency, M.-Y. Yang, C.-Y. Huang, T.H.T. Chiu, K.-C. Chang, M.-N. Lin, L.-Y. Chen, A. Hu Jul 2020

Using Gas Chromatography And Mass Spectrometry To Determine 25-Hydroxyvitamin D Levels For Clinical Assessment Of Vitamin D Deficiency, M.-Y. Yang, C.-Y. Huang, T.H.T. Chiu, K.-C. Chang, M.-N. Lin, L.-Y. Chen, A. Hu

Journal of Food and Drug Analysis

Vitamin D is responsible for multiple metabolic functions in humans. Rickets are the most common disease caused by vitamin D deficiency. It is caused by poor calcium intake resulting in poor serum-ionized calcium. The purpose of this study is to develop a rapid, sensitive, and feasible method to determine the 25-hydroxy-vitamin D3 (25(OH)D3) levels in blood samples for clinical assessment. In this study, gas chromatography coupled mass spectrometry with trimethylsilyl derivatization (TMS-GC-MS) is the most suitable protocol for quantitative analyses of 25(OH)D3. Performance of method was evaluated and compared with liquid chromatography and immunoassay. Method validation has been carried out …

Go to article

Current Status Of Maldi-Tof Mass Spectrometry In Clinical Microbiology, T.-Y. Hou, C. Chiang-Ni, S.-H. Teng Jul 2020

Current Status Of Maldi-Tof Mass Spectrometry In Clinical Microbiology, T.-Y. Hou, C. Chiang-Ni, S.-H. Teng

Journal of Food and Drug Analysis

Mass spectrometry (MS) is a type of analysis used to determine what molecules make up a sample, based on the mass spectrum that are created by the ions. Mass spectrometers are able to perform traditional target analyte identification and quantitation; however, they may also be used within a clinical setting for the rapid identification of bacteria. The causative agent in sepsis is changed over time, and clinical decisions affecting the management of infections are often based on the outcomes of bacterial identification. Therefore, it is essential that such identifications are performed quickly and interpreted correctly. Matrix-assisted laser desorption/ionization-time of flight …

Go to article

Utilizing Proteomic Approach To Identify Nuclear Translocation Related Serine Kinase Phosphorylation Site Of Gnmt As Downstream Effector For Benzo[A]Pyrene, M.-H. Yang, C.-C. Liao, J.-H. Hung, X.-T. Lai, C.-H. Yen, Y.-M.A. Chen Jul 2020

Utilizing Proteomic Approach To Identify Nuclear Translocation Related Serine Kinase Phosphorylation Site Of Gnmt As Downstream Effector For Benzo[A]Pyrene, M.-H. Yang, C.-C. Liao, J.-H. Hung, X.-T. Lai, C.-H. Yen, Y.-M.A. Chen

Journal of Food and Drug Analysis

Glycine N-methyltransferase (GNMT) protein is highly expressed in certain tissues, such as liver, pancreas, and prostate. GNMT serves multiple roles which include a methyl group transfer enzyme and a liver tumor suppressor. Benzo(a)pyrene (BaP), a family member of polycyclic aromatic hydrocarbon (PAH), is a known environmental carcinogen found in coal tar, tobacco smoke, barbecued food and incomplete combustion of auto fuel. BaP recruits cytochrome P450 to transform itself into benzo(a)pyrene-7,8-diol-9,10-epoxide (B(a)PDE), which covalently interacts with DNA causing tumorigenesis. BaP can be detoxified through GNMT and induces GNMT translocation into the cellular nucleus. GNMT translocation is accompanied by phosphorylation, but the …

Go to article

Detection Of Bpde-Dna Adducts In Human Umbilical Cord Blood By Lc-Ms/Ms Analysis, L. Guo, X. Jiang, H.-Y. Tian, S.-J. Yao, B.-Y. Li, R.-J. Zhang, S.-S. Zhang, X. Sun Jul 2020

Detection Of Bpde-Dna Adducts In Human Umbilical Cord Blood By Lc-Ms/Ms Analysis, L. Guo, X. Jiang, H.-Y. Tian, S.-J. Yao, B.-Y. Li, R.-J. Zhang, S.-S. Zhang, X. Sun

Journal of Food and Drug Analysis

Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to …

Go to article

A Highly Sensitive Ultra-High Performance Liquid Chromatography/Tandem Mass Spectrometry Method With In-Source Fragmentation For Rapid Quantification Of Raspberry Ketone, B. Yuan, D. Zhao, R. Du, D. Kshatriya, N.T. Bello, J.E. Simon, Q. Wu Jul 2020

A Highly Sensitive Ultra-High Performance Liquid Chromatography/Tandem Mass Spectrometry Method With In-Source Fragmentation For Rapid Quantification Of Raspberry Ketone, B. Yuan, D. Zhao, R. Du, D. Kshatriya, N.T. Bello, J.E. Simon, Q. Wu

Journal of Food and Drug Analysis

Raspberry ketone (RK) is the characteristic aromatic compound in raspberry (Rubus idaeus L.) with wide applications as food additive and anti-obesity agent. However, quantification of RK has presented difficulties in MS detection and reliable LC-MS method for RK analysis in literature is in limit to date. In order to facilitate quality control of raspberry derived products and RK metabolomics study, this study aimed to develop a validated and sensitive UHPLC-MS/MS method. Strong in-source fragmentation was noted and the fragmental ion of 107 m/z produced was selected as the precursor ion for MRM detection, and as such the electrospray ionization performance …

Go to article

Nelumbo Nucifera Leaves Extract Attenuate The Pathological Progression Of Diabetic Nephropathy In High-Fat Diet-Fed And Streptozotocin-Induced Diabetic Rats, H.-W. Chen, M.-Y. Yang, T.-W. Hung, Y.-C. Chang, C.-J. Wang Jul 2020

Nelumbo Nucifera Leaves Extract Attenuate The Pathological Progression Of Diabetic Nephropathy In High-Fat Diet-Fed And Streptozotocin-Induced Diabetic Rats, H.-W. Chen, M.-Y. Yang, T.-W. Hung, Y.-C. Chang, C.-J. Wang

Journal of Food and Drug Analysis

Diabetic nephropathy is not only a common and severe microvascular complication of diabetes mellitus but also the leading cause of renal failure. Lotus (Nelumbo nucifera) possesses antioxidative and anticancer properties. The present study aimed to investigate the antidiabetic and renoprotective effects of N. nucifera leaf extract (NLE) in a rat model of type 2 diabetic mellitus. Male Sprague–Dawley rats with type 2 diabetes induced by a high-fat diet (HFD)/streptozotocin (STZ) were treated with NLE at dosages of 0.5% and 1% (w/w) daily for 6 weeks. At the end of the experimental period, body weight, serum glucose levels, insulin levels, and …

Go to article

Risk Of Adverse Cardiovascular Events With Use Of Inhaled Long-Acting Bronchodilators In Management Of Chronic Obstructive Pulmonary Disease, M.-T. Wang, J.-H. Lai, C.-L. Tsai, J.-T. Liou Jul 2020

Risk Of Adverse Cardiovascular Events With Use Of Inhaled Long-Acting Bronchodilators In Management Of Chronic Obstructive Pulmonary Disease, M.-T. Wang, J.-H. Lai, C.-L. Tsai, J.-T. Liou

Journal of Food and Drug Analysis

Inhaled long-acting bronchodilators, including long-acting β2 agonists (LABAs) and long-acting muscarinic antagonists (LAMAs) are the mainstay therapy in the treatment of chronic obstructive pulmonary disease (COPD), a disease that poses a heavy burden on morbidity and mortality worldwide. Use of LABAs and LAMAs in patients with COPD, however, has been concerned about an increased risk of adverse cardiovascular events, despite inconsistent findings reported from randomized controlled trials (RCTs) and observational studies. In this review, we detailed the relevant evidence generated from RCTs and observational studies with respect to the risk of cardiovascular disease with use of LABAs and LAMAs in …

Go to article

A Nutraceutical Extract From Inula Viscosa Leaves: Uhplc-Hr-Ms/Ms Based Polyphenol Profile, And Antioxidant And Cytotoxic Activities, N. Brahmi-Chendouh, S. Piccolella, G. Crescente, F. Pacifico, L. Boulekbache, S. Hamri-Zeghichi, S. Akkal, K. Madani, S. Pacifico Jul 2020

A Nutraceutical Extract From Inula Viscosa Leaves: Uhplc-Hr-Ms/Ms Based Polyphenol Profile, And Antioxidant And Cytotoxic Activities, N. Brahmi-Chendouh, S. Piccolella, G. Crescente, F. Pacifico, L. Boulekbache, S. Hamri-Zeghichi, S. Akkal, K. Madani, S. Pacifico

Journal of Food and Drug Analysis

Nowadays, advanced extraction techniques and highly sensitive metabolic profiling methods are effectively employed to get new information on plant chemical constituents. Among them wild medicinal plants or their parts, with large and ancient use in folk medicine, are investigated for their potential functional use and cultivation. In this context, Inula viscosa leaves engaged our attention. A simple experimental design, based on Soxhlet extraction and chromatographic fractionation, allowed us to obtain the investigated polyphenol fraction (IvE). UHPLC-HRMS analyses revealed shikimoyl depsides of caffeic acid and unusual dihydrobenzofuran lignans as main secondary metabolites. These compounds, together with cinchonain-type phenols, and hydroxycinnamoyl flavonol …

Go to article

Direct Injection High Performance Liquid Chromatography Coupled To Data Independent Acquisition Mass Spectrometry For The Screening Of Antibiotics In Honey, Eyken A. Von, D. Furlong, S. Arooni, F. Butterworth, J.-F. Roy, J. Zweigenbaum, S. Bayen Jul 2020

Direct Injection High Performance Liquid Chromatography Coupled To Data Independent Acquisition Mass Spectrometry For The Screening Of Antibiotics In Honey, Eyken A. Von, D. Furlong, S. Arooni, F. Butterworth, J.-F. Roy, J. Zweigenbaum, S. Bayen

Journal of Food and Drug Analysis

The targeted analysis of veterinary drug residues in honey traditionally involves a series of extraction and purification steps prior to quantification with high performance liquid chromatography coupled to high resolution or tandem mass spectrometry. These steps, designed to separate the target analytes from interferences, are generally time-consuming and costly. In addition, traditional cleanup steps are likely to eliminate other compounds whose analysis could prove decisive in current or future assessment of the honey sample. Alternatively, direct injection without complex sample preparation steps has been introduced for the fast analysis of trace compounds in environmental and food matrices. The aim of …

Go to article

Revisit Gut Microbiota And Its Impact On Human Health And Disease, R.-X. Ding, W.-R. Goh, R.-N. Wu, X.-Q. Yue, X. Luo, W.W.T. Khine, J.-R. Wu, Y.-K. Lee Jul 2020

Revisit Gut Microbiota And Its Impact On Human Health And Disease, R.-X. Ding, W.-R. Goh, R.-N. Wu, X.-Q. Yue, X. Luo, W.W.T. Khine, J.-R. Wu, Y.-K. Lee

Journal of Food and Drug Analysis

Trillions of microbes have evolved with and continue to live on human beings. With the rapid advances in tools and technology in recent years, new knowledge and insight in cross-talk between the microbes and their hosts have gained. It is the aim of this work to critically review and summarize recent literature reports on the role of microbiota and mechanisms involved in the progress and development of major human diseases, which include obesity, hypertension, cardiovascular disease, diabetes, cancer, Inflammatory Bowel Disease (IBD), gout, depression and arthritis, as well as infant health and longevity. © 2019

Go to article

Next Generation Probiotics In Disease Amelioration, C.-J. Chang, T.-L. Lin, Y.-L. Tsai, T.-R. Wu, W.-F. Lai, C.-C. Lu, H.-C. Lai Jul 2020

Next Generation Probiotics In Disease Amelioration, C.-J. Chang, T.-L. Lin, Y.-L. Tsai, T.-R. Wu, W.-F. Lai, C.-C. Lu, H.-C. Lai

Journal of Food and Drug Analysis

Studies on the role of gut commensal bacteria in health development have rapidly attracted much more attention beyond the classical pathogens over the last decade. Many important reports have highlighted the changes in the gut microbiota (dysbiosis) are closely related to development of intra- and extra-intestinal, chronic inflammation related diseases such as colitis, obesity/metabolic syndromes, diabetes mellitus, liver diseases, cardiovascular diseases and also cancer and neurodegenerative diseases. To circumvent these difficulties, the strategy of modulating the structure of the gut microbiota has been under intensive study and shed more light on amelioration of these inflammation related diseases. While traditional probiotics …

Go to article

Psychobiotics In Mental Health, Neurodegenerative And Neurodevelopmental Disorders, L.-H. Cheng, Y.-W. Liu, C.-C. Wu, S. Wang, Y.-C. Tsai Jul 2020

Psychobiotics In Mental Health, Neurodegenerative And Neurodevelopmental Disorders, L.-H. Cheng, Y.-W. Liu, C.-C. Wu, S. Wang, Y.-C. Tsai

Journal of Food and Drug Analysis

Psychobiotics are a group of probiotics that affect the central nervous system (CNS) related functions and behaviors mediated by the gut-brain-axis (GBA) via immune, humoral, neural, and metabolic pathways to improve not only the gastrointestinal (GI) function but also the antidepressant and anxiolytic capacity. As a novel class of probiotics, the application of psychobiotics has led researchers to focus on a new area in neuroscience. In the past five years, some psychobiotics strains were reported to inhibit inflammation and decreased cortisol levels, resulting in an amelioration of the symptoms of anxiety and depression. Psychobiotics are efficacious in improving neurodegenerative and …

Go to article

First Prev Next Last